BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146584.2 - phase: 0 /pseudo
(1452 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BF003873 similar to GP|14715222|em putative polyprotein {Cicer a... 232 3e-61
AL366725 164 2e-40
TC93153 similar to GP|14715220|emb|CAC44106. gag polyprotein {Ci... 84 3e-16
BG644699 similar to PIR|T07863|T078 probable polyprotein - pinea... 59 2e-08
BG587176 weakly similar to PIR|G84493|G84 probable retroelement ... 55 2e-07
BG452991 PIR|A25875|A25 histone H4 - Tetrahymena thermophila, pa... 39 0.019
AW573695 weakly similar to GP|10177655|db gb|AAD43171.1~gene_id:... 38 0.033
BG644693 weakly similar to GP|18767374|g Putative 22 kDa kafirin... 36 0.095
TC86022 homologue to GP|19423939|gb|AAL87312.1 putative RNA heli... 34 0.47
TC84839 similar to GP|19386827|dbj|BAB86205. hypothetical protei... 33 1.1
BG583840 GP|20146556|gb ATP synthase alpha subunit {Clethra barb... 32 1.4
TC78940 similar to SP|P22196|PER2_ARAHY Cationic peroxidase 2 pr... 32 1.4
AW257152 similar to PIR|D86409|D864 hypothetical protein AAF9842... 32 1.8
TC93137 weakly similar to PIR|JQ2285|JQ2285 nodulin-26 - soybean... 32 1.8
BG647396 weakly similar to PIR|T50791|T50 hypothetical protein T... 32 2.3
BG644345 weakly similar to GP|19697333|gb putative protein poten... 32 2.3
CA989682 similar to GP|3702336|gb| 3-methyl-2-oxobutanoate hydro... 31 3.1
TC88440 weakly similar to GP|7293965|gb|AAF49324.1| Eip74EF gene... 31 3.1
TC79570 similar to PIR|F86427|F86427 auxin response factor 6 (AR... 31 3.1
TC77718 similar to GP|11072026|gb|AAG28905.1 F12A21.16 {Arabidop... 31 3.1
>BF003873 similar to GP|14715222|em putative polyprotein {Cicer arietinum},
partial (82%)
Length = 559
Score = 232 bits (591), Expect(2) = 3e-61
Identities = 115/127 (90%), Positives = 120/127 (93%)
Frame = +2
Query: 1326 KEVDPKFIGPYQILERVGTVAYRVGLPPHLSNLHNVFHVSQLRKYVPDPSHVIQSDDVQV 1385
K++ +FIGPYQI ERVGTVAYRVGLPPHL NLH+VFHVSQLRKYVPDPSHVIQSDDVQV
Sbjct: 26 KKLTVRFIGPYQISERVGTVAYRVGLPPHLLNLHDVFHVSQLRKYVPDPSHVIQSDDVQV 205
Query: 1386 RDNLTVETLPVRIDDRKVKTLRGKEIPLVRVVWTGATGESLTWELESKMLESYPELFA*G 1445
RDNLTVETLPVRIDDRKVKTLRGKEIPLVRVVW A GESLTWELESKM+ESYPELFA*G
Sbjct: 206 RDNLTVETLPVRIDDRKVKTLRGKEIPLVRVVWDRANGESLTWELESKMVESYPELFA*G 385
Query: 1446 KFSRTKI 1452
KFSRTKI
Sbjct: 386 KFSRTKI 406
Score = 23.5 bits (49), Expect(2) = 3e-61
Identities = 9/9 (100%), Positives = 9/9 (100%)
Frame = +1
Query: 1321 TCFEVKEVD 1329
TCFEVKEVD
Sbjct: 10 TCFEVKEVD 36
>AL366725
Length = 485
Score = 164 bits (415), Expect = 2e-40
Identities = 101/161 (62%), Positives = 119/161 (73%), Gaps = 1/161 (0%)
Frame = +3
Query: 147 SSTRTILLRMLSSRDASSSRTV*GPTSRGRLDTNSLEFFKIWSIAAGSMKRIRRLTTR** 206
SS RT+LLR+LSSR+ASSSR V*GPTSRG+ DTNS EF I I A S +RI RL TR *
Sbjct: 3 SSIRTMLLRLLSSRNASSSRMV*GPTSRGQSDTNSSEFSLI**IPAESTRRILRLMTRL* 182
Query: 207 MRGRARDSRVVLSRTVPLLIKGNRRWSMLGGLRRRM-LQRLCVSIVVRKATKATSALRKS 265
M GR R +RV LS VPLLI+ NR WSM+G LRRRM L+RL VS + RKAT+ L++S
Sbjct: 183 MSGRPRANRVALSLIVPLLIRANREWSMIGVLRRRMLLRRLSVSTMARKATRVMFVLKRS 362
Query: 266 RSVSGVARKVML*LIAIVRTLFALIATERVTLVHSVLSLRG 306
R+VSGV R+V+L*LIA L A IAT+RV LV + SLRG
Sbjct: 363 RNVSGVTRRVIL*LIASEMILCASIATKRVILVPNASSLRG 485
>TC93153 similar to GP|14715220|emb|CAC44106. gag polyprotein {Cicer
arietinum}, partial (8%)
Length = 516
Score = 84.3 bits (207), Expect = 3e-16
Identities = 60/147 (40%), Positives = 82/147 (54%)
Frame = +3
Query: 13 NNLTQMLVRMLKLGCWRLS*RRTLQLSKDVMTLMEPRRGLRRLRGFSGLCSALRIRKCGL 72
N+ ++++ +++ GC RLS* Q S+ M LM R G RRL+ +S C+ LR R+C L
Sbjct: 63 NSYLRLILVVMEPGC*RLS*GIIHQHSRVGMLLMVLRSG*RRLKEYSESCNVLRHRRCSL 242
Query: 73 VLIS*PRKLMTGGLLFCLPLGRRELL*LGLFSGESS*GDTFRKMFAGRSRSSSLS*SKET 132
* ++ M GGL+ CL R L * G SG S F++M R R + S*++ T
Sbjct: 243 GRTC*LKRPMIGGLVCCLFWSRMMLW*PGPCSGRSFWAGIFQRMSGVRKRLNFWS*NRVT 422
Query: 133 CLW*SMLPSLWNCQSSTRTILLRMLSS 159
CL SML SLWN ST + R LSS
Sbjct: 423 CLSQSMLQSLWNLPHSTLITVRRQLSS 503
>BG644699 similar to PIR|T07863|T078 probable polyprotein - pineapple
retrotransposon dea1 (fragment), partial (5%)
Length = 231
Score = 58.5 bits (140), Expect = 2e-08
Identities = 22/49 (44%), Positives = 38/49 (76%)
Frame = +2
Query: 1331 KFIGPYQILERVGTVAYRVGLPPHLSNLHNVFHVSQLRKYVPDPSHVIQ 1379
++IGP+++++R+G VAY + LPP LS +H VFHVS ++Y D +++I+
Sbjct: 77 RYIGPFEVIKRIGEVAYELALPPGLSGVHPVFHVSMFKRYHGDGNYIIR 223
>BG587176 weakly similar to PIR|G84493|G84 probable retroelement pol
polyprotein [imported] - Arabidopsis thaliana, partial
(1%)
Length = 729
Score = 55.5 bits (132), Expect = 2e-07
Identities = 26/54 (48%), Positives = 35/54 (64%)
Frame = -1
Query: 1389 LTVETLPVRIDDRKVKTLRGKEIPLVRVVWTGATGESLTWELESKMLESYPELF 1442
L +ET PVRI DR K +R K I +V++VW + E +TWE E++M YPE F
Sbjct: 717 LDLETRPVRILDRMEKAMRKKPIQMVKIVWDCSGREEITWETEARMKADYPEWF 556
>BG452991 PIR|A25875|A25 histone H4 - Tetrahymena thermophila, partial (33%)
Length = 560
Score = 38.5 bits (88), Expect = 0.019
Identities = 33/78 (42%), Positives = 42/78 (53%)
Frame = +2
Query: 936 QRVRIS*TV*RYEFGLRMVTSECEIGYAED***IPEKYQGSTES*CKVCGLVGC*RSD*R 995
+ + + *TV R EFGL V +ECEIG ED I YQ +E C+V SD*R
Sbjct: 53 RELELP*TVQRLEFGL*SVATECEIGDVEDQQRIFG*YQRGSEGGCEVGRFNVWE*SD*R 232
Query: 996 Q*F*NR*PRCVEIPRKNL 1013
*F*+ * R V + N+
Sbjct: 233 W*F*S**SRSVAVSG*NM 286
>AW573695 weakly similar to GP|10177655|db
gb|AAD43171.1~gene_id:MAC12.15~similar to unknown
protein {Arabidopsis thaliana}, partial (38%)
Length = 652
Score = 37.7 bits (86), Expect = 0.033
Identities = 28/96 (29%), Positives = 51/96 (52%), Gaps = 12/96 (12%)
Frame = +3
Query: 329 EVLAILIILL*LLLLILVLRIVL-----------LLSIVFLLWVLICLI*MERWLSKLQL 377
+ L L ++ L+L++VL+++L LL ++ LLW ++C +*+ WL L L
Sbjct: 165 QTLLFLRGVIDFLMLLMVLKVLLNVILMSPSLERLLFLILLLWFMLCWL*LLCWLFMLLL 344
Query: 378 RVR*LLL-SYV*NVLCLYLVVISKWI*FVYL*AVWM 412
R R ++L + LC LV+ I ++ * +W+
Sbjct: 345 RPRLVVLEEWDLTSLCPLLVLRRLRILIIFR*KLWL 452
>BG644693 weakly similar to GP|18767374|g Putative 22 kDa kafirin cluster;
Ty3-Gypsy type {Oryza sativa}, partial (15%)
Length = 716
Score = 36.2 bits (82), Expect = 0.095
Identities = 33/80 (41%), Positives = 43/80 (53%)
Frame = +3
Query: 603 ARLI*GQVITRLK*KMKICRRQLSERVMVTMNIKLCLLVLPMHLECLWSI*IASSMHFWI 662
+RL *G T + * +K+ + LSE MVTM CLL + LW+ *I S
Sbjct: 333 SRLT*GWDNTNIG**VKMFLKLLSELDMVTMKS**CLLGKQIPRWHLWN**IEFSKITST 512
Query: 663 GLWLCLSMIF*FTPRLKKNM 682
L L MIF*+TPR+K +M
Sbjct: 513 H**LFLVMIF*YTPRMKMSM 572
>TC86022 homologue to GP|19423939|gb|AAL87312.1 putative RNA helicase
{Arabidopsis thaliana}, partial (89%)
Length = 1982
Score = 33.9 bits (76), Expect = 0.47
Identities = 24/61 (39%), Positives = 41/61 (66%), Gaps = 3/61 (4%)
Frame = -2
Query: 323 TRIV*SEVLAILIILL*LLLLILVLRIVLLLSIV--FLLWVLICLI*MERW-LSKLQLRV 379
T++V + L +L++LL LLL++L++ IVLL+ I+ L +L+ L+ + W L K +RV
Sbjct: 343 TQLVPPQPLLLLLLLLILLLILLLILIVLLMLILHHVPLHILLLLLILMMWSLLKPGVRV 164
Query: 380 R 380
R
Sbjct: 163 R 161
>TC84839 similar to GP|19386827|dbj|BAB86205. hypothetical protein~similar
to Arabidopsis thaliana chromosome 5 T6I14_10, partial
(20%)
Length = 819
Score = 32.7 bits (73), Expect = 1.1
Identities = 33/101 (32%), Positives = 53/101 (51%), Gaps = 7/101 (6%)
Frame = -1
Query: 263 RKSRSVSGVARKVML*LIAIVRTLFALIATERVTLVHSVLSLRGRRLQVES-------LL 315
R++R G RK L + +R +A R L+ R RR VE LL
Sbjct: 438 RRTRGTRGTTRKK---LRSEIRRRSGRLAHHRRRLLRH----RRRRSTVEIRTGT*LLLL 280
Query: 316 *LVLRQRTRIV*SEVLAILIILL*LLLLILVLRIVLLLSIV 356
L+++ R ++ +L +LI+LL LLLL+LVL ++L+L ++
Sbjct: 279 LLMVKMRRLLL---LLVLLILLLLLLLLLLVLLVLLVLRVM 166
Score = 31.2 bits (69), Expect = 3.1
Identities = 16/37 (43%), Positives = 28/37 (75%)
Frame = -1
Query: 330 VLAILIILL*LLLLILVLRIVLLLSIVFLLWVLICLI 366
+L +L++ + LLL+LVL I+LLL ++ LL +L+ L+
Sbjct: 288 LLLLLMVKMRRLLLLLVLLILLLLLLLLLLVLLVLLV 178
>BG583840 GP|20146556|gb ATP synthase alpha subunit {Clethra barbinervis},
partial (17%)
Length = 585
Score = 32.3 bits (72), Expect = 1.4
Identities = 15/32 (46%), Positives = 25/32 (77%)
Frame = +2
Query: 330 VLAILIILL*LLLLILVLRIVLLLSIVFLLWV 361
+L +L++LL LLLL+L+L ++LLL I +W+
Sbjct: 11 LLLLLLLLLLLLLLLLLLLLLLLLYIYIYMWL 106
Score = 32.0 bits (71), Expect = 1.8
Identities = 15/31 (48%), Positives = 26/31 (83%)
Frame = +2
Query: 333 ILIILL*LLLLILVLRIVLLLSIVFLLWVLI 363
+L++LL LLLL+L+L ++LLL ++ LL++ I
Sbjct: 2 LLLLLLLLLLLLLLLLLLLLLLLLLLLYIYI 94
Score = 30.0 bits (66), Expect = 6.8
Identities = 14/35 (40%), Positives = 27/35 (77%)
Frame = +2
Query: 331 LAILIILL*LLLLILVLRIVLLLSIVFLLWVLICL 365
L +L++LL LLLL+L+L ++LLL ++ +++ + L
Sbjct: 2 LLLLLLLLLLLLLLLLLLLLLLLLLLLYIYIYMWL 106
Score = 29.6 bits (65), Expect = 8.9
Identities = 15/33 (45%), Positives = 24/33 (72%)
Frame = +2
Query: 340 LLLLILVLRIVLLLSIVFLLWVLICLI*MERWL 372
LLLL+L+L ++LLL ++ LL +L+ I + WL
Sbjct: 8 LLLLLLLLLLLLLLLLLLLLLLLLLYIYIYMWL 106
>TC78940 similar to SP|P22196|PER2_ARAHY Cationic peroxidase 2 precursor (EC
1.11.1.7). [Peanut] {Arachis hypogaea}, partial (92%)
Length = 1272
Score = 32.3 bits (72), Expect = 1.4
Identities = 26/85 (30%), Positives = 52/85 (60%), Gaps = 6/85 (7%)
Frame = +3
Query: 275 VML*LIAIVRTLFALIATERVTLVHSVLSLRGRRLQVESLL*LV------LRQRTRIV*S 328
++L*L+ + F ++ ++ VTL+ S+ + R+ ++L+* V LRQ +R+
Sbjct: 312 ILL*LLVYLGCTFMIVLSKVVTLLFSLPAQAPRKQHFQTLV*EVLKLLRMLRQNSRLH-V 488
Query: 329 EVLAILIILL*LLLLILVLRIVLLL 353
VL++++I L LLL+I + +V+L+
Sbjct: 489 PVLSLVLISLLLLLVIPLF*VVVLV 563
>AW257152 similar to PIR|D86409|D864 hypothetical protein AAF98424.1
[imported] - Arabidopsis thaliana, partial (20%)
Length = 587
Score = 32.0 bits (71), Expect = 1.8
Identities = 18/72 (25%), Positives = 41/72 (56%)
Frame = +2
Query: 285 TLFALIATERVTLVHSVLSLRGRRLQVESLL*LVLRQRTRIV*SEVLAILIILL*LLLLI 344
T+ ++ + T V + +E+ L +V+ ++T ++ ++ + I++L LLLL+
Sbjct: 164 TIITIVYHNQDTSAKPVKDIGQEVEHLETFLLVVVAEKTNVLNDQITLLQILILLLLLLL 343
Query: 345 LVLRIVLLLSIV 356
L L IVL+ +++
Sbjct: 344 LQLLIVLIQTLL 379
>TC93137 weakly similar to PIR|JQ2285|JQ2285 nodulin-26 - soybean, partial
(47%)
Length = 633
Score = 32.0 bits (71), Expect = 1.8
Identities = 17/54 (31%), Positives = 32/54 (58%)
Frame = -2
Query: 342 LLILVLRIVLLLSIVFLLWVLICLI*MERWLSKLQLRVR*LLLSYV*NVLCLYL 395
L + L ++L L+ + L L+C++ +E + LQL ++*L Y+ +LC Y+
Sbjct: 479 LKLACLLLILKLACLLLSLKLVCMLILESYRQVLQLWLQ*LDQLYISTILCKYV 318
>BG647396 weakly similar to PIR|T50791|T50 hypothetical protein T30N20_90 -
Arabidopsis thaliana, partial (38%)
Length = 787
Score = 31.6 bits (70), Expect = 2.3
Identities = 25/72 (34%), Positives = 42/72 (57%), Gaps = 2/72 (2%)
Frame = +1
Query: 297 LVHSVLSLRGRRLQVESLL*LVLRQRTRIV*SEVLAILIILL*LLLLILVLRIVLLLSIV 356
L+ S+L L GR L +LR R V +L++ + +LLL+++L +++L IV
Sbjct: 337 LILSLLELLGRCLL------RLLRWEVR*VWLLR*CVLLV*VLVLLLLMLLSMLVLQGIV 498
Query: 357 --FLLWVLICLI 366
FL+W+LIC +
Sbjct: 499 LRFLVWLLICRV 534
>BG644345 weakly similar to GP|19697333|gb putative protein potential
transcriptional repressor Not4hp - Mus musculus, partial
(7%)
Length = 635
Score = 31.6 bits (70), Expect = 2.3
Identities = 21/61 (34%), Positives = 41/61 (66%)
Frame = -3
Query: 306 GRRLQVESLL*LVLRQRTRIV*SEVLAILIILL*LLLLILVLRIVLLLSIVFLLWVLICL 365
G ++Q+ L *L+L Q+ ++ +L + ++LL LLL+L+L+++LLL ++ L L+ L
Sbjct: 489 GEKVQLLLL**LMLLQQLLLL---LLQLQLLLLLKLLLLLLLKLLLLLLLLLLKLKLLLL 319
Query: 366 I 366
+
Sbjct: 318 L 316
>CA989682 similar to GP|3702336|gb| 3-methyl-2-oxobutanoate
hydroxy-methyl-transferase {Arabidopsis thaliana},
partial (39%)
Length = 489
Score = 31.2 bits (69), Expect = 3.1
Identities = 15/37 (40%), Positives = 29/37 (77%)
Frame = +1
Query: 330 VLAILIILL*LLLLILVLRIVLLLSIVFLLWVLICLI 366
V+ +L +LL*+ L +V+R++++L ++FLL +ICL+
Sbjct: 286 VMILLCLLL*MKCLFIVVRLLVVLKLLFLL--VICLL 390
>TC88440 weakly similar to GP|7293965|gb|AAF49324.1| Eip74EF gene product
{Drosophila melanogaster}, partial (5%)
Length = 711
Score = 31.2 bits (69), Expect = 3.1
Identities = 29/78 (37%), Positives = 50/78 (63%)
Frame = -3
Query: 307 RRLQVESLL*LVLRQRTRIV*SEVLAILIILL*LLLLILVLRIVLLLSIVFLLWVLICLI 366
R+L+++SLL +L Q E++ +L+++ *LLL++L L + LLL +V LL V++ L+
Sbjct: 406 RQLKLKSLL--LLHQM------EMMMVLLLVG*LLLVVL-LLVGLLLLVV*LLLVVLLLV 254
Query: 367 *MERWLSKLQLRVR*LLL 384
+ L LQL + L+L
Sbjct: 253 GLLLVLHLLQLVLHLLVL 200
>TC79570 similar to PIR|F86427|F86427 auxin response factor 6 (ARF6)
[imported] - Arabidopsis thaliana, partial (15%)
Length = 1143
Score = 31.2 bits (69), Expect = 3.1
Identities = 15/30 (50%), Positives = 26/30 (86%)
Frame = -3
Query: 334 LIILL*LLLLILVLRIVLLLSIVFLLWVLI 363
L++LL*LLLL+L++ ++LLL ++ LL +L+
Sbjct: 667 LLLLL*LLLLLLLMLLLLLLLLLLLLLLLL 578
Score = 30.4 bits (67), Expect = 5.2
Identities = 17/37 (45%), Positives = 28/37 (74%)
Frame = -3
Query: 330 VLAILIILL*LLLLILVLRIVLLLSIVFLLWVLICLI 366
+L +L +LL LLL++L+L ++LLL ++ LL +I LI
Sbjct: 667 LLLLL*LLLLLLLMLLLLLLLLLLLLLLLLLHMIILI 557
>TC77718 similar to GP|11072026|gb|AAG28905.1 F12A21.16 {Arabidopsis
thaliana}, partial (14%)
Length = 1089
Score = 31.2 bits (69), Expect = 3.1
Identities = 21/46 (45%), Positives = 32/46 (68%), Gaps = 2/46 (4%)
Frame = +2
Query: 330 VLAILIILL*LLLLILVLRIVLLLSIVFL--LWVLICLI*MERWLS 373
++ I ILL L L++L+L++ LLL I+FL L++ I LI + R LS
Sbjct: 344 LMFIFGILLVLFLVVLLLKLWLLLKIIFLANLFIRILLIEILRLLS 481
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.365 0.164 0.591
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 48,156,223
Number of Sequences: 36976
Number of extensions: 758943
Number of successful extensions: 11480
Number of sequences better than 10.0: 51
Number of HSP's better than 10.0 without gapping: 2789
Number of HSP's successfully gapped in prelim test: 483
Number of HSP's that attempted gapping in prelim test: 8067
Number of HSP's gapped (non-prelim): 4137
length of query: 1452
length of database: 9,014,727
effective HSP length: 108
effective length of query: 1344
effective length of database: 5,021,319
effective search space: 6748652736
effective search space used: 6748652736
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 14 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 36 (21.6 bits)
S2: 65 (29.6 bits)
Medicago: description of AC146584.2