BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146573.11 + phase: 2 /pseudo
(1025 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
AW299024 similar to PIR|D96655|D9 hypothetical protein F16M19.19... 423 e-119
BQ752355 similar to PIR|A00694|RN DNA-directed RNA polymerase (E... 45 1e-04
BF636556 weakly similar to PIR|T06754|T06 DNA-directed RNA polym... 38 0.017
BI310476 similar to GP|8777343|db DNA-directed RNA polymerase II... 33 0.43
BF640151 similar to PIR|D96495|D964 unknown protein [imported] -... 33 0.56
BM779750 similar to PIR|T06036|T06 hypothetical protein T24A18.1... 30 3.6
BM779741 weakly similar to GP|13872902|db putative protein kinas... 30 6.2
TC80734 similar to GP|8885568|dbj|BAA97498.1 general negative tr... 29 8.1
>AW299024 similar to PIR|D96655|D9 hypothetical protein F16M19.19 [imported]
- Arabidopsis thaliana, partial (11%)
Length = 677
Score = 423 bits (1087), Expect(2) = e-119
Identities = 211/212 (99%), Positives = 211/212 (99%)
Frame = +3
Query: 764 NADLPGTLTRRLMFFMRDMYDAYDGTVRNLYGNQLIQFSYDAEEDSSCDSYFREDIIGGE 823
NADL GTLTRRLMFFMRDMYDAYDGTVRNLYGNQLIQFSYDAEEDSSCDSYFREDIIGGE
Sbjct: 39 NADLSGTLTRRLMFFMRDMYDAYDGTVRNLYGNQLIQFSYDAEEDSSCDSYFREDIIGGE 218
Query: 824 PVGALSACAVSEAAYSALSQPISLLEASPLLNLKNVLECGSRKKGGDQTVSLFLSEKIGK 883
PVGALSACAVSEAAYSALSQPISLLEASPLLNLKNVLECGSRKKGGDQTVSLFLSEKIGK
Sbjct: 219 PVGALSACAVSEAAYSALSQPISLLEASPLLNLKNVLECGSRKKGGDQTVSLFLSEKIGK 398
Query: 884 QRNGFEYAALEVKNYLERYMFSNIVSTVMIIFTPQHCSQVKYSPWVCHFHLDKENVTRRK 943
QRNGFEYAALEVKNYLERYMFSNIVSTVMIIFTPQHCSQVKYSPWVCHFHLDKENVTRRK
Sbjct: 399 QRNGFEYAALEVKNYLERYMFSNIVSTVMIIFTPQHCSQVKYSPWVCHFHLDKENVTRRK 578
Query: 944 LEVHSIIDSLYQQYDSFRRESKVILPSLKISS 975
LEVHSIIDSLYQQYDSFRRESKVILPSLKISS
Sbjct: 579 LEVHSIIDSLYQQYDSFRRESKVILPSLKISS 674
Score = 26.2 bits (56), Expect(2) = e-119
Identities = 11/11 (100%), Positives = 11/11 (100%)
Frame = +2
Query: 752 HSVTNRDSSFS 762
HSVTNRDSSFS
Sbjct: 2 HSVTNRDSSFS 34
>BQ752355 similar to PIR|A00694|RN DNA-directed RNA polymerase (EC 2.7.7.6)
III 160K chain - yeast (Saccharomyces cerevisiae),
partial (12%)
Length = 535
Score = 45.1 bits (105), Expect = 1e-04
Identities = 39/150 (26%), Positives = 70/150 (46%), Gaps = 2/150 (1%)
Frame = -2
Query: 660 KDNSFLAMFKAGSKGNLLKLVQHSMCLGLQHSLVRLSYRIPRELSCAGWNSQKGLHSMEM 719
++N+ L M K+GSKG+ + + Q +G Q I + G+ + H +
Sbjct: 471 RNNAPLVMAKSGSKGSEINVAQMVALVGQQI--------IGGKRVADGFQDRTLPHFYKN 316
Query: 720 SSDTLEPKQSYIAHAVVESSFLTGLNPLECFVHSVTNRDSSFSDNADLP--GTLTRRLMF 777
+ P + ++A+ SF +GL P E H+++ R+ G ++RRLM
Sbjct: 315 APQP--PSKGFVAN-----SFYSGLLPTEFIFHAMSGREGLVDTAVKTAETGYMSRRLMK 157
Query: 778 FMRDMYDAYDGTVRNLYGNQLIQFSYDAEE 807
+ D+ YD TVR G ++QF + A++
Sbjct: 156 SLEDLSTQYDDTVRT-SGGGIVQFQFGADK 70
>BF636556 weakly similar to PIR|T06754|T06 DNA-directed RNA polymerase I 190K
chain homolog F15B8.150 - Arabidopsis thaliana, partial
(4%)
Length = 657
Score = 38.1 bits (87), Expect = 0.017
Identities = 26/119 (21%), Positives = 52/119 (42%), Gaps = 18/119 (15%)
Frame = +1
Query: 16 ISYLCYLVLCLQEKISALEINAPGQVTCSDLGLP--------------NSSYGCTTCG-S 60
I + + L +E + + + C+++G P ++ C +CG S
Sbjct: 112 IESVAFSFLTNEELLKSSRVKVTDTNLCNNIGHPVRGGLYDPAFGPLLDNRSNCESCGMS 291
Query: 61 KDRKSCEGHFGAIKFPFTILHPYFMAEIAEILQKICPAC---KSIRQEIRVKVCLLPIV 116
KD C GHFG I+ + +P + +IL + C +C ++ R E++ + L ++
Sbjct: 292 KDH--CSGHFGHIELVSPVYNPLMFLFLGKILNRTCFSCHYFRASRDEVKRRASQLELI 462
>BI310476 similar to GP|8777343|db DNA-directed RNA polymerase II largest
chain {Arabidopsis thaliana}, partial (11%)
Length = 673
Score = 33.5 bits (75), Expect = 0.43
Identities = 40/180 (22%), Positives = 71/180 (39%), Gaps = 37/180 (20%)
Frame = +2
Query: 424 GGNLLSLSQDSLTAAHLLLEDGVGFNIYQVQQLEMLCNK--------ELTSPAIFKAPSS 475
G L++ +QD LT++ L+ Y ++C+ +L +PAI K
Sbjct: 2 GEILVASTQDFLTSSFLITRKDT---FYDRSSFSLICSYMGDGMDPIDLPTPAIIKP--- 163
Query: 476 NTPFWSGKQLFSMLL-------------PSKFDYAFPSND--------------VFVKDG 508
WSGKQLFS+LL + +Y +D V+ ++
Sbjct: 164 -VELWSGKQLFSILLRPHANVRVYVNLTVKEKNYGSKLDDREREFKTMCPDDGFVYFRNS 340
Query: 509 ELISCSESSGWL-RDSENNVFQSLVEHFQG-KILDVLHGAQKALCEWLSMTGFSVSLSDL 566
ELIS L +++ +F L+ ++ ++ K W+ GFS+ + D+
Sbjct: 341 ELISGQLGKVTLGNGNKDGLFSVLLRDYKSDAAASCMNRLAKLSARWIGNHGFSIGIDDV 520
>BF640151 similar to PIR|D96495|D964 unknown protein [imported] - Arabidopsis
thaliana, partial (6%)
Length = 588
Score = 33.1 bits (74), Expect = 0.56
Identities = 24/77 (31%), Positives = 40/77 (51%)
Frame = +3
Query: 945 EVHSIIDSLYQQYDSFRRESKVILPSLKISSNEGGETSVGNEKDGEDCITVAVVEDSKNS 1004
E+ ++ DSLYQ + + RR + + LP + V + KD D A ++++KNS
Sbjct: 132 ELEALSDSLYQSHTTARRAASLALPRTSVPF-------VPSAKDDND---TAKLDNNKNS 281
Query: 1005 VQLDSVRKSLIPFLLQT 1021
+ S R SL P+ +T
Sbjct: 282 NKPRSRRMSLSPWKSKT 332
>BM779750 similar to PIR|T06036|T06 hypothetical protein T24A18.10 -
Arabidopsis thaliana, partial (18%)
Length = 863
Score = 30.4 bits (67), Expect = 3.6
Identities = 17/45 (37%), Positives = 24/45 (52%), Gaps = 2/45 (4%)
Frame = +3
Query: 93 QKICPA-CKSIRQ-EIRVKVCLLPIVKVLFHAYSVLNHSLDTIIS 135
QK+CP CK I K +LP+V L A ++ SLD ++S
Sbjct: 135 QKMCPRICKLINNPNFLAKAAILPVVSALSQAGAIAPQSLDNLLS 269
>BM779741 weakly similar to GP|13872902|db putative protein kinase Xa21
{Oryza sativa (japonica cultivar-group)}, partial (11%)
Length = 815
Score = 29.6 bits (65), Expect = 6.2
Identities = 38/153 (24%), Positives = 61/153 (39%), Gaps = 7/153 (4%)
Frame = +3
Query: 394 LHGYIPQSVGARVELNELVALDRQLING--QSGGNLLSLSQDSLTAAHLLLEDGVGFNIY 451
L G +P G+ ++L EL L+ S GNL SL + S HL E + +++
Sbjct: 321 LIGRVPAYFGSMMQLTELSLGHNNLVGTIPSSLGNLSSLEKLSFLQNHL--EGSIPYSLG 494
Query: 452 QVQQLEMLC----NKELTSP-AIFKAPSSNTPFWSGKQLFSMLLPSKFDYAFPSNDVFVK 506
++ L L N P +++ + SG LF + PS D FP+ + F
Sbjct: 495 RLSVLTWLSLSLNNLSGEIPHSLYNLSNIQIFSISGNNLFGSI-PSNIDLVFPNLEQFFI 671
Query: 507 DGELISCSESSGWLRDSENNVFQSLVEHFQGKI 539
IS + S + VF + G +
Sbjct: 672 GSNQISATFPSSISNLTRLQVFDIAYNNINGAL 770
>TC80734 similar to GP|8885568|dbj|BAA97498.1 general negative transcription
regulator-like {Arabidopsis thaliana}, partial (7%)
Length = 1094
Score = 29.3 bits (64), Expect = 8.1
Identities = 17/65 (26%), Positives = 34/65 (52%), Gaps = 1/65 (1%)
Frame = +3
Query: 415 DRQLINGQSGGNLLSLSQDSLTAAHLLLEDGV-GFNIYQVQQLEMLCNKELTSPAIFKAP 473
D ++ N + +L SQ + + ++ LED V F+ +++ E++CN L + + +
Sbjct: 198 DNKVCNSKEQYDLKLDSQSEVASGYVELEDDVTSFDSQRLKDPEVVCNSYLPNSSFPRVA 377
Query: 474 SSNTP 478
S N P
Sbjct: 378 SHNNP 392
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.328 0.141 0.432
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 33,219,890
Number of Sequences: 36976
Number of extensions: 493903
Number of successful extensions: 3137
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 2301
Number of HSP's successfully gapped in prelim test: 116
Number of HSP's that attempted gapping in prelim test: 808
Number of HSP's gapped (non-prelim): 2465
length of query: 1025
length of database: 9,014,727
effective HSP length: 106
effective length of query: 919
effective length of database: 5,095,271
effective search space: 4682554049
effective search space used: 4682554049
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)
S2: 63 (28.9 bits)
Medicago: description of AC146573.11