BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146571.4 + phase: 0
(494 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC79568 weakly similar to GP|21689747|gb|AAM67517.1 unknown prot... 680 0.0
BG458048 GP|15983468|gb At1g54990/F14C21_5 {Arabidopsis thaliana... 327 6e-90
AL370647 183 1e-46
TC79570 similar to PIR|F86427|F86427 auxin response factor 6 (AR... 39 0.005
TC90757 similar to GP|17528996|gb|AAL38708.1 unknown protein {Ar... 37 0.013
TC81467 similar to GP|15912319|gb|AAL08293.1 AT4g12830/T20K18_18... 37 0.013
TC77584 similar to PIR|T07145|T07145 epoxide hydrolase homolog -... 36 0.039
TC92746 similar to PIR|T07145|T07145 epoxide hydrolase homolog -... 35 0.050
TC77524 similar to PIR|T07145|T07145 epoxide hydrolase homolog -... 35 0.066
TC77525 similar to PIR|T07145|T07145 epoxide hydrolase homolog -... 35 0.066
TC87847 similar to GP|15929245|gb|AAH15068.1 Similar to adaptor-... 35 0.066
BE204345 similar to GP|15126717|gb| Similar to ectoplacental con... 35 0.066
TC87846 similar to GP|5821151|dbj|BAA83717.1 RNA binding protein... 35 0.066
TC93531 similar to PIR|E86271|E86271 protein F16A14.4 [imported]... 34 0.11
TC78177 similar to PIR|T07145|T07145 epoxide hydrolase homolog -... 34 0.15
TC84484 similar to GP|20161418|dbj|BAB90342. phospholipase-like ... 33 0.19
BG451137 similar to GP|15912319|gb AT4g12830/T20K18_180 {Arabido... 33 0.19
BF647289 weakly similar to PIR|T01316|T013 epoxide hydrolase hom... 33 0.19
TC88515 weakly similar to PIR|T45731|T45731 epoxide hydrolase-li... 33 0.19
TC90911 similar to PIR|T02681|T02681 probable zinc transporter A... 32 0.56
>TC79568 weakly similar to GP|21689747|gb|AAM67517.1 unknown protein
{Arabidopsis thaliana}, partial (25%)
Length = 1192
Score = 680 bits (1754), Expect = 0.0
Identities = 347/378 (91%), Positives = 353/378 (92%), Gaps = 1/378 (0%)
Frame = +3
Query: 1 MAIITEEQESDQQQQQQKQKQKQKQQHSNKTKSKTQKPSTTQPQQTNPLSFWFYFTLSIS 60
MAIITEEQESDQQQQQQKQKQKQKQQHSNKTKSKTQKPSTTQPQQTNPLSFWFYFTLSIS
Sbjct: 18 MAIITEEQESDQQQQQQKQKQKQKQQHSNKTKSKTQKPSTTQPQQTNPLSFWFYFTLSIS 197
Query: 61 LVTLFFLFTSSPLSSSSPQQWFLTLPTTLRQHYSNGRTIKVQIHPNQQPIHLFTFQLDPT 120
LVTLFFLFTSSPLSSSSPQQWFLTLPTTLRQHYSNGRTIKVQIHPNQQPIHLFTFQLDPT
Sbjct: 198 LVTLFFLFTSSPLSSSSPQQWFLTLPTTLRQHYSNGRTIKVQIHPNQQPIHLFTFQLDPT 377
Query: 121 IPNPSETVLILHGQALSSYSYRNLIQSLSTQGVRVIAIDLPGSGFSDKSVEVSVEGLDGI 180
IPNPSETVLILHGQALSSYSYRNLIQSLSTQGVRVIAIDLPGSGFSDKSVEVSVEGLDGI
Sbjct: 378 IPNPSETVLILHGQALSSYSYRNLIQSLSTQGVRVIAIDLPGSGFSDKSVEVSVEGLDGI 557
Query: 181 FGRFSYVYSEIKEKGFFWAFDQIVETGQIPYEEVLARMSKRKVNKPIDLGPEEIGKVLGE 240
FGRFSYVYSEIKEKGFFWAFDQIVETGQIPYEEVLARMSKRKVNKPIDLGPEEIGKVLGE
Sbjct: 558 FGRFSYVYSEIKEKGFFWAFDQIVETGQIPYEEVLARMSKRKVNKPIDLGPEEIGKVLGE 737
Query: 241 VIGTLGLAPVHLVLHDSALGFTANWVSENSDLVSSLTLVDTPVPPSNLGAFPIWVLDVPL 300
VIGTLGLAPVHLVLHDSALGFTANWVSENSDLVSSLTLVDTPVPPSNLGAFPIWVLDVPL
Sbjct: 738 VIGTLGLAPVHLVLHDSALGFTANWVSENSDLVSSLTLVDTPVPPSNLGAFPIWVLDVPL 917
Query: 301 IREVVLGFPYVFAKVVNFYCSKRIGGLDADAHRVLLKSGDGRKAVVAIGKNLNSSFDLTE 360
IREVVLGFPYVFAKVVNFYCSKRIGGLDADAH ++ +++ +
Sbjct: 918 IREVVLGFPYVFAKVVNFYCSKRIGGLDADAH*GAVEVWGWKESGCGYWQEFEF*L*FN* 1097
Query: 361 WGC-SDRLKDMPMQLIWS 377
G SDRLKDMPMQLIWS
Sbjct: 1098MGVGSDRLKDMPMQLIWS 1151
Score = 76.6 bits (187), Expect = 2e-14
Identities = 42/66 (63%), Positives = 44/66 (66%), Gaps = 8/66 (12%)
Frame = +1
Query: 333 RVLLKSGDGRKAVVAIGKNLNSSFDLTEWG--------CSDRLKDMPMQLIWSSDWSEEW 384
RVLLKSGDGRKAVVAIGKNLNSSFDLTEWG C + DWSEEW
Sbjct: 1015 RVLLKSGDGRKAVVAIGKNLNSSFDLTEWGLVLID*KICQCN*SGL-------GDWSEEW 1173
Query: 385 SSEGNR 390
S+EGNR
Sbjct: 1174 STEGNR 1191
>BG458048 GP|15983468|gb At1g54990/F14C21_5 {Arabidopsis thaliana}, partial
(8%)
Length = 550
Score = 327 bits (838), Expect = 6e-90
Identities = 152/155 (98%), Positives = 152/155 (98%)
Frame = -1
Query: 340 DGRKAVVAIGKNLNSSFDLTEWGCSDRLKDMPMQLIWSSDWSEEWSSEGNRVAGALPRAK 399
DGRKAVVAIGKNLNSSFDL EWGCSDRLKDMPMQL WSSDWSEEWSSEGNRVAGALPRAK
Sbjct: 550 DGRKAVVAIGKNLNSSFDLXEWGCSDRLKDMPMQLXWSSDWSEEWSSEGNRVAGALPRAK 371
Query: 400 FVTHSGGRWAQEDVAVEIAEKISQFVSSLPKTVRKVEQEPPIPDHVQKMFDEAKSDDGHD 459
FVTH GGRWAQEDVAVEIAEKISQFVSSLPKTVRKVEQEPPIPDHVQKMFDEAKSDDGHD
Sbjct: 370 FVTHXGGRWAQEDVAVEIAEKISQFVSSLPKTVRKVEQEPPIPDHVQKMFDEAKSDDGHD 191
Query: 460 HHQGHSHGHDRLGEAHIHEAGYMDAYGIGHEPNDW 494
HHQGHSHGHDRLGEAHIHEAGYMDAYGIGHEPNDW
Sbjct: 190 HHQGHSHGHDRLGEAHIHEAGYMDAYGIGHEPNDW 86
>AL370647
Length = 384
Score = 183 bits (465), Expect = 1e-46
Identities = 84/84 (100%), Positives = 84/84 (100%)
Frame = +3
Query: 411 EDVAVEIAEKISQFVSSLPKTVRKVEQEPPIPDHVQKMFDEAKSDDGHDHHQGHSHGHDR 470
EDVAVEIAEKISQFVSSLPKTVRKVEQEPPIPDHVQKMFDEAKSDDGHDHHQGHSHGHDR
Sbjct: 48 EDVAVEIAEKISQFVSSLPKTVRKVEQEPPIPDHVQKMFDEAKSDDGHDHHQGHSHGHDR 227
Query: 471 LGEAHIHEAGYMDAYGIGHEPNDW 494
LGEAHIHEAGYMDAYGIGHEPNDW
Sbjct: 228 LGEAHIHEAGYMDAYGIGHEPNDW 299
>TC79570 similar to PIR|F86427|F86427 auxin response factor 6 (ARF6)
[imported] - Arabidopsis thaliana, partial (15%)
Length = 1143
Score = 38.9 bits (89), Expect = 0.005
Identities = 33/136 (24%), Positives = 55/136 (40%), Gaps = 5/136 (3%)
Frame = +2
Query: 8 QESDQQQQQQKQKQKQKQQHSNKTKSKTQKPSTTQPQQTNPLSFWFYFTLSISLVTLFFL 67
Q+ QQQQQQ+Q+Q+Q+ Q + +S+ Q+ Q +N +S T+
Sbjct: 575 QQQQQQQQQQQQQQQQQHQQQQQQQSQQQQQVVDHQQMSNAVS------------TMSQF 718
Query: 68 FTSSPLSSSSPQQWFLTLPTTLRQHYSNGRTIK-----VQIHPNQQPIHLFTFQLDPTIP 122
++ P S+ P Q ++ SNG + + N + HL F P
Sbjct: 719 VSAPPSQSTQPMQAISSIGHQQNFSDSNGNPVSPLHNMLGSFSNDETSHLLNF------P 880
Query: 123 NPSETVLILHGQALSS 138
P+ V + A S
Sbjct: 881 RPNSWVPVQSSTARPS 928
>TC90757 similar to GP|17528996|gb|AAL38708.1 unknown protein {Arabidopsis
thaliana}, partial (41%)
Length = 717
Score = 37.4 bits (85), Expect = 0.013
Identities = 18/42 (42%), Positives = 23/42 (53%)
Frame = +3
Query: 127 TVLILHGQALSSYSYRNLIQSLSTQGVRVIAIDLPGSGFSDK 168
T++ LHG S+SYR ++ L G A D G GFSDK
Sbjct: 399 TIVFLHGAPTQSFSYRVVMSELGDAGFHCFAPDWIGFGFSDK 524
>TC81467 similar to GP|15912319|gb|AAL08293.1 AT4g12830/T20K18_180
{Arabidopsis thaliana}, partial (86%)
Length = 1199
Score = 37.4 bits (85), Expect = 0.013
Identities = 24/87 (27%), Positives = 43/87 (48%), Gaps = 9/87 (10%)
Frame = +2
Query: 120 TIPNPSETVLILHGQALSSYSYRNLIQSLSTQGVRVIAIDLPGSGFSDK---------SV 170
++ NPS V+++HG +YSYR ++ LS + R + D G GFSDK ++
Sbjct: 422 SVDNPS--VILIHGFPSQAYSYRKVLPVLS-KDYRAVTFDWLGFGFSDKPQPKYGFDYTL 592
Query: 171 EVSVEGLDGIFGRFSYVYSEIKEKGFF 197
+ V L+ + + + +G+F
Sbjct: 593 DEYVSSLESLIDELAVTKVSLVVQGYF 673
>TC77584 similar to PIR|T07145|T07145 epoxide hydrolase homolog - soybean,
partial (70%)
Length = 819
Score = 35.8 bits (81), Expect = 0.039
Identities = 21/46 (45%), Positives = 27/46 (58%)
Frame = +2
Query: 128 VLILHGQALSSYSYRNLIQSLSTQGVRVIAIDLPGSGFSDKSVEVS 173
VL LHG YS+R+ I +LS+ G R +A DL G G +D VS
Sbjct: 158 VLFLHGFPELWYSWRHQIVALSSLGYRAVAPDLRGYGDTDAPSSVS 295
>TC92746 similar to PIR|T07145|T07145 epoxide hydrolase homolog - soybean,
partial (44%)
Length = 523
Score = 35.4 bits (80), Expect = 0.050
Identities = 19/46 (41%), Positives = 26/46 (56%)
Frame = +2
Query: 128 VLILHGQALSSYSYRNLIQSLSTQGVRVIAIDLPGSGFSDKSVEVS 173
VL LHG YS+R+ I +L + G R +A DL G G +D +S
Sbjct: 17 VLFLHGFPELWYSWRHQISALGSLGYRAVAPDLRGYGDTDAPASIS 154
>TC77524 similar to PIR|T07145|T07145 epoxide hydrolase homolog - soybean,
partial (81%)
Length = 1262
Score = 35.0 bits (79), Expect = 0.066
Identities = 19/46 (41%), Positives = 26/46 (56%)
Frame = +2
Query: 128 VLILHGQALSSYSYRNLIQSLSTQGVRVIAIDLPGSGFSDKSVEVS 173
VL LHG YS+R+ I +L + G R +A DL G G +D +S
Sbjct: 95 VLFLHGFPELWYSWRHQIAALGSLGYRAVAPDLRGYGDTDAPASIS 232
>TC77525 similar to PIR|T07145|T07145 epoxide hydrolase homolog - soybean,
partial (88%)
Length = 1457
Score = 35.0 bits (79), Expect = 0.066
Identities = 19/46 (41%), Positives = 26/46 (56%)
Frame = +2
Query: 128 VLILHGQALSSYSYRNLIQSLSTQGVRVIAIDLPGSGFSDKSVEVS 173
VL LHG YS+R+ I +L + G R +A DL G G +D +S
Sbjct: 185 VLFLHGFPELWYSWRHQIAALGSLGYRAVAPDLRGYGDTDAPASIS 322
>TC87847 similar to GP|15929245|gb|AAH15068.1 Similar to adaptor-related
protein complex AP-3 beta 1 subunit {Mus musculus},
partial (2%)
Length = 931
Score = 35.0 bits (79), Expect = 0.066
Identities = 12/46 (26%), Positives = 27/46 (58%)
Frame = +1
Query: 5 TEEQESDQQQQQQKQKQKQKQQHSNKTKSKTQKPSTTQPQQTNPLS 50
+EE+E D++ + ++++ + +T SK KP++ P + P+S
Sbjct: 193 SEEEEGDEEDGSSSEDEEEEDNQTQQTPSKDSKPASKNPPPSTPIS 330
>BE204345 similar to GP|15126717|gb| Similar to ectoplacental cone invasive
trophoblast giant cells extraembryonic ectoderm and,
partial (2%)
Length = 325
Score = 35.0 bits (79), Expect = 0.066
Identities = 12/46 (26%), Positives = 27/46 (58%)
Frame = +1
Query: 5 TEEQESDQQQQQQKQKQKQKQQHSNKTKSKTQKPSTTQPQQTNPLS 50
+EE+E D++ + ++++ + +T SK KP++ P + P+S
Sbjct: 85 SEEEEGDEEDGSSSEDEEEEDNQTQQTPSKDSKPASKNPPPSTPIS 222
>TC87846 similar to GP|5821151|dbj|BAA83717.1 RNA binding protein {Homo
sapiens}, partial (2%)
Length = 550
Score = 35.0 bits (79), Expect = 0.066
Identities = 12/46 (26%), Positives = 27/46 (58%)
Frame = +1
Query: 5 TEEQESDQQQQQQKQKQKQKQQHSNKTKSKTQKPSTTQPQQTNPLS 50
+EE+E D++ + ++++ + +T SK KP++ P + P+S
Sbjct: 175 SEEEEGDEEDGSSSEDEEEEDNQTQQTPSKDSKPASKNPPPSTPIS 312
>TC93531 similar to PIR|E86271|E86271 protein F16A14.4 [imported] -
Arabidopsis thaliana, partial (19%)
Length = 637
Score = 34.3 bits (77), Expect = 0.11
Identities = 17/49 (34%), Positives = 24/49 (48%)
Frame = +3
Query: 119 PTIPNPSETVLILHGQALSSYSYRNLIQSLSTQGVRVIAIDLPGSGFSD 167
P + N +++LHG S +R L G+ AID+ G GFSD
Sbjct: 234 PLVQNKENPIVLLHGFDSSCLEWRYTYPLLEEAGIETWAIDILGWGFSD 380
>TC78177 similar to PIR|T07145|T07145 epoxide hydrolase homolog - soybean,
partial (89%)
Length = 1037
Score = 33.9 bits (76), Expect = 0.15
Identities = 19/46 (41%), Positives = 26/46 (56%)
Frame = +2
Query: 128 VLILHGQALSSYSYRNLIQSLSTQGVRVIAIDLPGSGFSDKSVEVS 173
VL LHG YS+R+ I +L + G R +A DL G G +D +S
Sbjct: 110 VLFLHGFPELWYSWRHQIAALGSLGYRAVAPDLRGYGDTDVPSSIS 247
>TC84484 similar to GP|20161418|dbj|BAB90342. phospholipase-like protein
{Oryza sativa (japonica cultivar-group)}, partial (38%)
Length = 656
Score = 33.5 bits (75), Expect = 0.19
Identities = 28/113 (24%), Positives = 43/113 (37%), Gaps = 1/113 (0%)
Frame = +2
Query: 82 FLTLPTTLRQHYSNGRTIKVQIHPNQQPIHLFTFQLDPTIPNPSETVLILHGQALSSYSY 141
F T LR Y G N + + LFT + P +P V + HG + +
Sbjct: 119 FTTKVMDLRYEYQEG------YWKNSRGMRLFTCKWLPISSSPKALVFLCHGYGMECSGF 280
Query: 142 -RNLIQSLSTQGVRVIAIDLPGSGFSDKSVEVSVEGLDGIFGRFSYVYSEIKE 193
+ + + L++ G V +D G G S V + D + S Y I E
Sbjct: 281 MKEIGEKLASAGYAVFGMDYEGHGHS-AGVRCYITKFDNVVNDCSNFYKSICE 436
>BG451137 similar to GP|15912319|gb AT4g12830/T20K18_180 {Arabidopsis
thaliana}, partial (32%)
Length = 631
Score = 33.5 bits (75), Expect = 0.19
Identities = 19/48 (39%), Positives = 28/48 (57%)
Frame = +3
Query: 120 TIPNPSETVLILHGQALSSYSYRNLIQSLSTQGVRVIAIDLPGSGFSD 167
++ NPS V+++HG +YSYR ++ LS + R D G GFSD
Sbjct: 489 SVDNPS--VILIHGFPSQAYSYRKVLPVLS-KDYRAXTFDWLGFGFSD 623
>BF647289 weakly similar to PIR|T01316|T013 epoxide hydrolase homolog
T14P8.15 - Arabidopsis thaliana, partial (24%)
Length = 682
Score = 33.5 bits (75), Expect = 0.19
Identities = 20/55 (36%), Positives = 27/55 (48%)
Frame = +2
Query: 128 VLILHGQALSSYSYRNLIQSLSTQGVRVIAIDLPGSGFSDKSVEVSVEGLDGIFG 182
VL+LHG YS+R+ + L+ G +A DL G G SD + L I G
Sbjct: 107 VLLLHGFPEIWYSWRHQLNYLAQNGYHAVAPDLRGYGDSDSPINSDSYTLHHIVG 271
>TC88515 weakly similar to PIR|T45731|T45731 epoxide hydrolase-like protein
- Arabidopsis thaliana, partial (51%)
Length = 771
Score = 33.5 bits (75), Expect = 0.19
Identities = 20/55 (36%), Positives = 27/55 (48%)
Frame = +2
Query: 128 VLILHGQALSSYSYRNLIQSLSTQGVRVIAIDLPGSGFSDKSVEVSVEGLDGIFG 182
VL+LHG YS+R+ + L+ G +A DL G G SD + L I G
Sbjct: 227 VLLLHGFPEIWYSWRHQLNYLAQNGYHAVAPDLRGYGDSDSPINSDSYTLHHIVG 391
>TC90911 similar to PIR|T02681|T02681 probable zinc transporter At2g46800
[imported] - Arabidopsis thaliana, partial (45%)
Length = 671
Score = 32.0 bits (71), Expect = 0.56
Identities = 11/21 (52%), Positives = 12/21 (56%)
Frame = +3
Query: 455 DDGHDHHQGHSHGHDRLGEAH 475
D GH H H HGHD G +H
Sbjct: 585 DHGHGHGHDHXHGHDHHGHSH 647
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.317 0.134 0.402
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 16,112,449
Number of Sequences: 36976
Number of extensions: 259034
Number of successful extensions: 2713
Number of sequences better than 10.0: 111
Number of HSP's better than 10.0 without gapping: 2355
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2566
length of query: 494
length of database: 9,014,727
effective HSP length: 100
effective length of query: 394
effective length of database: 5,317,127
effective search space: 2094948038
effective search space used: 2094948038
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 60 (27.7 bits)
Medicago: description of AC146571.4