BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146569.8 - phase: 0
(166 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC92601 86 9e-18
AL389791 82 8e-17
BF520646 similar to GP|21741986|emb OSJNBa0060P14.7 {Oryza sativ... 40 3e-04
TC86391 similar to GP|4097571|gb|AAD09514.1| GMFP5 {Glycine max}... 37 0.005
TC76916 MtN4 35 0.014
BF644693 homologue to GP|2598599|emb MtN4 {Medicago truncatula},... 35 0.014
AJ389009 34 0.024
TC79211 similar to GP|4204793|gb|AAD10836.1| P-glycoprotein {Sol... 34 0.031
BQ150824 32 0.12
BI267312 32 0.12
TC83786 homologue to GP|16905421|gb|AAL31335.1 variable protein ... 32 0.12
TC78881 similar to PIR|D86236|D86236 protein F14N23.5 [imported]... 31 0.20
TC89128 similar to GP|21593748|gb|AAM65715.1 unknown {Arabidopsi... 31 0.26
AJ497365 similar to GP|7295709|gb|A CG15432 gene product {Drosop... 30 0.34
TC85575 similar to PIR|D86410|D86410 protein F3M18.16 [imported]... 30 0.34
AW774293 similar to GP|13881624|gb PPE family protein {Mycobacte... 30 0.45
TC79772 weakly similar to GP|20259023|gb|AAM14227.1 unknown prot... 30 0.45
AL386655 weakly similar to GP|20259023|gb| unknown protein {Arab... 30 0.58
TC88726 similar to GP|11994239|dbj|BAB01361. gb|AAD27719.1~gene_... 30 0.58
AW586908 weakly similar to GP|2104814|emb| pleiotropic effects o... 30 0.58
>TC92601
Length = 455
Score = 85.5 bits (210), Expect = 9e-18
Identities = 39/53 (73%), Positives = 48/53 (89%)
Frame = +3
Query: 8 GVTSVSLEGDDKDRVCVTGDNVDIVCLANQLKKKFNNVTILTTEEVKKKTEAE 60
GVTSVSLEG+DKD +CV GD+VD +CL NQLKKKFN+VTIL+ EEVKKK++A+
Sbjct: 297 GVTSVSLEGNDKDSICVIGDDVDTICLPNQLKKKFNSVTILSVEEVKKKSDAD 455
>AL389791
Length = 276
Score = 82.4 bits (202), Expect = 8e-17
Identities = 40/48 (83%), Positives = 43/48 (89%)
Frame = +3
Query: 8 GVTSVSLEGDDKDRVCVTGDNVDIVCLANQLKKKFNNVTILTTEEVKK 55
GVTSVSLEGDDKD++ VTGDNVD VCLAN LKKKFN VTIL+ EEVKK
Sbjct: 132 GVTSVSLEGDDKDKISVTGDNVDTVCLANMLKKKFNCVTILSVEEVKK 275
>BF520646 similar to GP|21741986|emb OSJNBa0060P14.7 {Oryza sativa}, partial
(26%)
Length = 590
Score = 40.4 bits (93), Expect = 3e-04
Identities = 23/57 (40%), Positives = 38/57 (66%)
Frame = +3
Query: 8 GVTSVSLEGDDKDRVCVTGDNVDIVCLANQLKKKFNNVTILTTEEVKKKTEAEKKKE 64
GV +++G++KD++ VTG+ +D V L + L+KKF + +++ V KTE EKK E
Sbjct: 126 GVEGTAIQGENKDQIEVTGEQIDSVRLTSLLRKKFCHAELVSVGPV-GKTE-EKKVE 290
>TC86391 similar to GP|4097571|gb|AAD09514.1| GMFP5 {Glycine max}, partial
(54%)
Length = 1353
Score = 36.6 bits (83), Expect = 0.005
Identities = 28/69 (40%), Positives = 43/69 (61%), Gaps = 5/69 (7%)
Frame = +1
Query: 8 GVTSVSLEGDDKDRVCVTGDNVDIVCLANQLKKKFN-NVTILTTEEVKKKTE----AEKK 62
GV +V+L+GD KD V V G ++ L LK+K NV I+ ++ ++K E EKK
Sbjct: 721 GVETVNLDGD-KDLVTVKG-TMEPKDLIEYLKEKLKRNVDIVPPKKEEEKKEKDGGGEKK 894
Query: 63 KEKEEEKKK 71
++KE+EKK+
Sbjct: 895 EKKEDEKKE 921
>TC76916 MtN4
Length = 1850
Score = 35.0 bits (79), Expect = 0.014
Identities = 22/72 (30%), Positives = 28/72 (38%), Gaps = 1/72 (1%)
Frame = +3
Query: 83 SCKCNNFNCHGKCNSCTKCESSKCDGTNCVTICSFKCGN-SKCDGNKCNGCTPKVTSPCF 141
SC CN C+ T C + C T+ TI + C N S C+ C T TS C
Sbjct: 867 SCTCNTT----LCS*ATNCFPTTCSSTSSCTITTTLCTNTSNCETTNCFSTTCSSTSSCA 1034
Query: 142 QRCPQWCTCSKC 153
T + C
Sbjct: 1035CNTTLCATTTYC 1070
Score = 29.6 bits (65), Expect = 0.58
Identities = 13/46 (28%), Positives = 19/46 (41%)
Frame = +3
Query: 95 CNSCTKCESSKCDGTNCVTICSFKCGNSKCDGNKCNGCTPKVTSPC 140
C + + CE++ C T C + S C + C T TS C
Sbjct: 963 CTNTSNCETTNCFSTTCSSTSSCACNTTLCATTTYCNSTNTNTSNC 1100
>BF644693 homologue to GP|2598599|emb MtN4 {Medicago truncatula}, partial
(79%)
Length = 621
Score = 35.0 bits (79), Expect = 0.014
Identities = 22/72 (30%), Positives = 28/72 (38%), Gaps = 1/72 (1%)
Frame = +1
Query: 83 SCKCNNFNCHGKCNSCTKCESSKCDGTNCVTICSFKCGN-SKCDGNKCNGCTPKVTSPCF 141
SC CN C+ T C + C T+ TI + C N S C+ C T TS C
Sbjct: 94 SCTCNTT----LCS*ATNCFPTTCSSTSSCTITTTLCTNTSNCETTNCFSTTCSSTSSCA 261
Query: 142 QRCPQWCTCSKC 153
T + C
Sbjct: 262 CNTTLCATTTYC 297
Score = 28.9 bits (63), Expect = 0.99
Identities = 12/40 (30%), Positives = 18/40 (45%), Gaps = 1/40 (2%)
Frame = +1
Query: 95 CNSCTKCESSKCDGTNCVTICSFKCGNSKC-DGNKCNGCT 133
C + + CE++ C T C + S C + C CN T
Sbjct: 190 CTNTSNCETTNCFSTTCSSTSSCACNTTLCATTTYCNSTT 309
>AJ389009
Length = 452
Score = 34.3 bits (77), Expect = 0.024
Identities = 15/34 (44%), Positives = 24/34 (70%)
Frame = +3
Query: 8 GVTSVSLEGDDKDRVCVTGDNVDIVCLANQLKKK 41
GVT V+LE + KDRV + G++VD + +L++K
Sbjct: 108 GVTMVTLEREGKDRVVIKGEDVDAARVTERLREK 209
>TC79211 similar to GP|4204793|gb|AAD10836.1| P-glycoprotein {Solanum
tuberosum}, partial (36%)
Length = 1667
Score = 33.9 bits (76), Expect = 0.031
Identities = 23/73 (31%), Positives = 32/73 (43%), Gaps = 2/73 (2%)
Frame = +3
Query: 85 KCNNFNCHGKCNSCTKCESS--KCDGTNCVTICSFKCGNSKCDGNKCNGCTPKVTSPCFQ 142
+C NF C C+ C +C+ S + + NC C++ C C C C + PC
Sbjct: 6 EC*NF-CKA-CS*CQQCQISHWRPNFGNCAEYCTYAC----C--LYCRLCVAVASCPCPH 161
Query: 143 RCPQWCTCSKCCA 155
C C CS C A
Sbjct: 162 CCIPCCCCSHCFA 200
>BQ150824
Length = 865
Score = 32.0 bits (71), Expect = 0.12
Identities = 17/55 (30%), Positives = 24/55 (42%), Gaps = 5/55 (9%)
Frame = -2
Query: 76 CHAVLHGSCKCNNFNCHGKCNSCTKCESSKCDGTNC-----VTICSFKCGNSKCD 125
C+AV+ SC N +C + +CE +CD NC + C G K D
Sbjct: 678 CNAVICSSCGTNLIDCIYEMARNVECEYGRCDIVNCRYRDIILYCIHVSGKDKRD 514
>BI267312
Length = 575
Score = 32.0 bits (71), Expect = 0.12
Identities = 15/36 (41%), Positives = 23/36 (63%)
Frame = -2
Query: 39 KKKFNNVTILTTEEVKKKTEAEKKKEKEEEKKKMIE 74
+ K V + E++K++T KKK+K E KKKMI+
Sbjct: 340 QNKTETVMGINEEQLKQRTRTMKKKKKREIKKKMIK 233
>TC83786 homologue to GP|16905421|gb|AAL31335.1 variable protein {African
swine fever virus}, partial (1%)
Length = 829
Score = 32.0 bits (71), Expect = 0.12
Identities = 18/54 (33%), Positives = 21/54 (38%)
Frame = +2
Query: 83 SCKCNNFNCHGKCNSCTKCESSKCDGTNCVTICSFKCGNSKCDGNKCNGCTPKV 136
S KC N + + C KC C S KCGNS + C G KV
Sbjct: 296 STKCQNCSVNAWKYKCPKCNFHSC---------SLKCGNSHKEKYSCEGIRSKV 430
>TC78881 similar to PIR|D86236|D86236 protein F14N23.5 [imported] -
Arabidopsis thaliana, partial (31%)
Length = 1710
Score = 31.2 bits (69), Expect = 0.20
Identities = 25/87 (28%), Positives = 34/87 (38%), Gaps = 10/87 (11%)
Frame = +3
Query: 77 HAVLHG-SCKCNNFNCHGKC--------NSCTK-CESSKCDGTNCVTICSFKCGNSKCDG 126
H VL C + C+ C ++C++ C SS CD + V CG S C G
Sbjct: 1017 HVVLRNIPCSSRDIRCNKLCGKTKQCGLHACSRICHSSPCDNQHVVQGIRTSCGQS-C-G 1190
Query: 127 NKCNGCTPKVTSPCFQRCPQWCTCSKC 153
C T+PC P C +C
Sbjct: 1191 APRRDCRHTCTAPCHPSSP--CPDRRC 1265
Score = 27.3 bits (59), Expect = 2.9
Identities = 24/109 (22%), Positives = 31/109 (28%), Gaps = 23/109 (21%)
Frame = +3
Query: 71 KMIEACHAVLHGSCKCNNFNCHGKCNSCTKCESSKCDGTNCVT---------------IC 115
K +E C + F C C C +C C +C
Sbjct: 567 KTVECCKKTMANE----KFTCEKPCGKKRNCGRHRCSERCCPLSNPNNILNATDSDPHLC 734
Query: 116 SF------KCGNSKCDGNKCNGCTPKVTSPCFQR--CPQWCTCSKCCAP 156
S +CG CD N +G P PC + C C K P
Sbjct: 735 SIPCGKKLRCGKHACDSNCHSGHCP----PCLETIFTDLTCACGKTSIP 869
>TC89128 similar to GP|21593748|gb|AAM65715.1 unknown {Arabidopsis
thaliana}, partial (43%)
Length = 810
Score = 30.8 bits (68), Expect = 0.26
Identities = 11/17 (64%), Positives = 12/17 (69%)
Frame = -1
Query: 80 LHGSCKCNNFNCHGKCN 96
L SCKC+ F CHG CN
Sbjct: 654 LQFSCKCSCFACHGNCN 604
>AJ497365 similar to GP|7295709|gb|A CG15432 gene product {Drosophila
melanogaster}, partial (51%)
Length = 625
Score = 30.4 bits (67), Expect = 0.34
Identities = 12/28 (42%), Positives = 21/28 (74%)
Frame = +1
Query: 47 ILTTEEVKKKTEAEKKKEKEEEKKKMIE 74
+ ++ +K KTE KKK+K +E+KK++E
Sbjct: 136 VANSKPLKLKTEGVKKKKKHKEEKKLLE 219
>TC85575 similar to PIR|D86410|D86410 protein F3M18.16 [imported] -
Arabidopsis thaliana, partial (15%)
Length = 1225
Score = 30.4 bits (67), Expect = 0.34
Identities = 24/92 (26%), Positives = 32/92 (34%), Gaps = 5/92 (5%)
Frame = -2
Query: 75 ACHAVL--HGSCKCNNF---NCHGKCNSCTKCESSKCDGTNCVTICSFKCGNSKCDGNKC 129
+CH+ L H C C F CH + C S C ++ C+ G C N C
Sbjct: 894 SCHSFLASHHCCNCCYF*SCICH*QQLCCCSYTSRSCCCSDFALSCNLLLGTLCC*KNLC 715
Query: 130 NGCTPKVTSPCFQRCPQWCTCSKCCAPYQPYC 161
C C C C CC+ + C
Sbjct: 714 CCC-----------CRNTCCC--CCSGFPWSC 658
>AW774293 similar to GP|13881624|gb PPE family protein {Mycobacterium
tuberculosis CDC1551}, partial (1%)
Length = 597
Score = 30.0 bits (66), Expect = 0.45
Identities = 18/61 (29%), Positives = 23/61 (37%), Gaps = 3/61 (4%)
Frame = -1
Query: 97 SCTKCESSKCDGTNCVTICSFKCGNSKCDGNKCNGCTPKVTSPCFQRCPQWC---TCSKC 153
+CT C S KC C + C KC+ C + CP C +C KC
Sbjct: 597 TCT-CPSMKC------------CCSCXCQLPKCSSCCSCINFSLSINCPDCCWKPSCIKC 457
Query: 154 C 154
C
Sbjct: 456 C 454
Score = 27.7 bits (60), Expect = 2.2
Identities = 16/47 (34%), Positives = 18/47 (38%), Gaps = 1/47 (2%)
Frame = -1
Query: 84 CKCNNF-NCHGKCNSCTKCESSKCDGTNCVTICSFKCGNSKCDGNKC 129
C+ F +C G CTKC C C CS C KC C
Sbjct: 456 CRLPKFTDCFGS-TCCTKCSMPGC----CCPKCSLPCCYPKCSRPSC 331
>TC79772 weakly similar to GP|20259023|gb|AAM14227.1 unknown protein
{Arabidopsis thaliana}, partial (33%)
Length = 1480
Score = 30.0 bits (66), Expect = 0.45
Identities = 11/28 (39%), Positives = 14/28 (49%)
Frame = +2
Query: 126 GNKCNGCTPKVTSPCFQRCPQWCTCSKC 153
G KC GC +V S C C C++C
Sbjct: 1115 GPKCRGCGERVASVVVLPCRHLCVCTEC 1198
>AL386655 weakly similar to GP|20259023|gb| unknown protein {Arabidopsis
thaliana}, partial (20%)
Length = 498
Score = 29.6 bits (65), Expect = 0.58
Identities = 12/36 (33%), Positives = 18/36 (49%), Gaps = 2/36 (5%)
Frame = +1
Query: 128 KCNGCTPKVTSPCFQRCPQWCTCSKCCAPYQ--PYC 161
KC GC +V + C C C++C A ++ P C
Sbjct: 139 KCRGCEKRVATVVVLPCRHLCVCTECDAHFRVCPVC 246
>TC88726 similar to GP|11994239|dbj|BAB01361.
gb|AAD27719.1~gene_id:MUJ8.10~similar to unknown protein
{Arabidopsis thaliana}, partial (58%)
Length = 2001
Score = 29.6 bits (65), Expect = 0.58
Identities = 17/58 (29%), Positives = 26/58 (44%), Gaps = 3/58 (5%)
Frame = +1
Query: 107 DGTNCV-TICSFKCGNSKCDGNKCNGCTPKVTSPCFQRC--PQWCTCSKCCAPYQPYC 161
DG + V T C F C ++C + C+P S C+ R W +C ++ YC
Sbjct: 1363 DGVSSVLTACQFLCNEARCSNRR---CSPHYPSSCWSRS*FGFW----RCIFSFKSYC 1515
>AW586908 weakly similar to GP|2104814|emb| pleiotropic effects on cellular
differentiation and slug behaviour {Dictyostelium
discoideum}, partial (8%)
Length = 668
Score = 29.6 bits (65), Expect = 0.58
Identities = 19/67 (28%), Positives = 23/67 (33%), Gaps = 5/67 (7%)
Frame = -3
Query: 95 CNSCTKCESSKCDGTNCVTICSFKCGN----SKCDGNKCNG-CTPKVTSPCFQRCPQWCT 149
C C C + C+ NC CG C G CN C C C +C
Sbjct: 624 CCCCCCCCCNFCNNCNCCCWSEMFCGTV*DICGCCGPLCNMLCCNNCNEDC---CN*FCC 454
Query: 150 CSKCCAP 156
C +C P
Sbjct: 453 CCRCKRP 433
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.320 0.134 0.459
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 8,829,241
Number of Sequences: 36976
Number of extensions: 205140
Number of successful extensions: 1944
Number of sequences better than 10.0: 146
Number of HSP's better than 10.0 without gapping: 1766
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1889
length of query: 166
length of database: 9,014,727
effective HSP length: 89
effective length of query: 77
effective length of database: 5,723,863
effective search space: 440737451
effective search space used: 440737451
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 54 (25.4 bits)
Medicago: description of AC146569.8