BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146569.7 + phase: 0
(166 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
AL389791 41 3e-04
TC92601 39 0.001
TC80117 weakly similar to PIR|T48360|T48360 farnesylated protein... 37 0.004
TC78881 similar to PIR|D86236|D86236 protein F14N23.5 [imported]... 34 0.024
TC85442 similar to GP|13543783|gb|AAH06040.1 Unknown (protein fo... 34 0.024
AW688848 homologue to PIR|T46027|T4 hypothetical protein T10K17.... 33 0.040
AW586908 weakly similar to GP|2104814|emb| pleiotropic effects o... 33 0.040
TC88253 weakly similar to GP|15810597|gb|AAL07186.1 unknown prot... 33 0.040
BG644345 weakly similar to GP|19697333|gb putative protein poten... 33 0.053
TC80931 similar to GP|14190783|gb|AAF65166.2 putative phloem tra... 33 0.053
TC92466 similar to GP|11141605|gb|AAG32022.1 LEUNIG {Arabidopsis... 33 0.069
TC80312 weakly similar to GP|19698847|gb|AAL91159.1 unknown prot... 33 0.069
TC81969 similar to GP|6523547|emb|CAB62280.1 hydroxyproline-rich... 33 0.069
TC80831 similar to GP|18542939|gb|AAK00428.2 Unknown protein {Or... 32 0.090
TC77769 similar to PIR|T06397|T06397 isoprenylated protein FP6 -... 32 0.090
TC78900 homologue to SP|P51819|HS83_PHANI Heat shock protein 83.... 32 0.090
TC77767 weakly similar to GP|22002137|gb|AAM88621.1 hypothetical... 32 0.090
TC79752 similar to GP|11994670|dbj|BAB02898. gene_id:K7P8.17~unk... 32 0.12
BG645312 similar to GP|16549312|dbj unnamed protein product {Hom... 32 0.12
AW774293 similar to GP|13881624|gb PPE family protein {Mycobacte... 31 0.20
>AL389791
Length = 276
Score = 40.8 bits (94), Expect = 3e-04
Identities = 19/19 (100%), Positives = 19/19 (100%)
Frame = +3
Query: 1 MLKKKFNCVTILSVEEVKK 19
MLKKKFNCVTILSVEEVKK
Sbjct: 219 MLKKKFNCVTILSVEEVKK 275
>TC92601
Length = 455
Score = 38.5 bits (88), Expect = 0.001
Identities = 18/23 (78%), Positives = 21/23 (91%)
Frame = +3
Query: 2 LKKKFNCVTILSVEEVKKKTEEE 24
LKKKFN VTILSVEEVKKK++ +
Sbjct: 387 LKKKFNSVTILSVEEVKKKSDAD 455
>TC80117 weakly similar to PIR|T48360|T48360 farnesylated protein-like -
Arabidopsis thaliana, partial (36%)
Length = 880
Score = 37.0 bits (84), Expect = 0.004
Identities = 21/64 (32%), Positives = 34/64 (52%)
Frame = +1
Query: 18 KKKTEEEKKKEEEKKKEEEKKKMMEACRTVLFGSCNSCCKTNCNGKCEKCTCGSAKCDGI 77
KK +EEKKK +E KK EEKK ++ + + + + +C+G +K K G+
Sbjct: 376 KKNHDEEKKKPDEDKKPEEKKPEEKSSKQSVQNTVVLKIRLHCDGCIQKIEKIILKIKGV 555
Query: 78 QCVT 81
+ VT
Sbjct: 556 ESVT 567
>TC78881 similar to PIR|D86236|D86236 protein F14N23.5 [imported] -
Arabidopsis thaliana, partial (31%)
Length = 1710
Score = 34.3 bits (77), Expect = 0.024
Identities = 41/165 (24%), Positives = 56/165 (33%), Gaps = 41/165 (24%)
Frame = +3
Query: 42 EACRTVLFGSCNSCCKTNCNGKC--------EKCTCGSAKCDGIQCVTICFKC-ENPKSC 92
+ C L + C T +G C +KC CGS + V C K N K
Sbjct: 447 QVCGKTLPCGMHHCTLTCHDGDCSPCSVLVSQKCRCGSTS----KTVECCKKTMANEKFT 614
Query: 93 CECKPIIKSCCNNKKCDDGCTPKKPPS---PKVQQCPQWCTCS-----RCYGYA----PY 140
CE K C +C + C P P+ P C+ RC +A +
Sbjct: 615 CEKPCGKKRNCGRHRCSERCCPLSNPNNILNATDSDPHLCSIPCGKKLRCGKHACDSNCH 794
Query: 141 QPYCNPC-----------------PPPNYMVCYDPNPE---PCSI 165
+C PC PPP + C P+P PCS+
Sbjct: 795 SGHCPPCLETIFTDLTCACGKTSIPPP--LPCGTPSPSCQLPCSV 923
>TC85442 similar to GP|13543783|gb|AAH06040.1 Unknown (protein for MGC:7642)
{Mus musculus}, partial (62%)
Length = 585
Score = 34.3 bits (77), Expect = 0.024
Identities = 18/26 (69%), Positives = 22/26 (84%), Gaps = 1/26 (3%)
Frame = +3
Query: 15 EEVKKKTEE-EKKKEEEKKKEEEKKK 39
EE KK+ E+ E+KKEE +KKEEEKKK
Sbjct: 165 EEPKKEEEKKEEKKEEGEKKEEEKKK 242
Score = 33.5 bits (75), Expect = 0.040
Identities = 16/36 (44%), Positives = 25/36 (69%)
Frame = +3
Query: 4 KKFNCVTILSVEEVKKKTEEEKKKEEEKKKEEEKKK 39
+K+ I+SV K+ ++E+ K+EE KKEEEKK+
Sbjct: 15 RKYWQADIISVGPAKEPEKKEEPKKEEPKKEEEKKE 122
Score = 31.6 bits (70), Expect = 0.15
Identities = 20/31 (64%), Positives = 22/31 (70%), Gaps = 6/31 (19%)
Frame = +3
Query: 15 EEVKK---KTEEEKK---KEEEKKKEEEKKK 39
EE KK K EEEKK K+EE KKEEEKK+
Sbjct: 105 EEEKKEEAKKEEEKKEGEKKEEPKKEEEKKE 197
Score = 30.8 bits (68), Expect = 0.26
Identities = 13/25 (52%), Positives = 21/25 (84%)
Frame = +3
Query: 15 EEVKKKTEEEKKKEEEKKKEEEKKK 39
E K++ ++E++K+EE KKEEEKK+
Sbjct: 78 EPKKEEPKKEEEKKEEAKKEEEKKE 152
Score = 30.4 bits (67), Expect = 0.34
Identities = 14/28 (50%), Positives = 20/28 (71%)
Frame = +3
Query: 15 EEVKKKTEEEKKKEEEKKKEEEKKKMME 42
E+ ++K EE +KKEEEKKKE ++E
Sbjct: 186 EKKEEKKEEGEKKEEEKKKEPAPDPVLE 269
Score = 29.6 bits (65), Expect = 0.58
Identities = 19/31 (61%), Positives = 22/31 (70%), Gaps = 6/31 (19%)
Frame = +3
Query: 15 EEVKK---KTEEEKKKE---EEKKKEEEKKK 39
EE KK K EEEKK+E EE+KKE EKK+
Sbjct: 75 EEPKKEEPKKEEEKKEEAKKEEEKKEGEKKE 167
Score = 29.3 bits (64), Expect = 0.76
Identities = 12/24 (50%), Positives = 21/24 (87%)
Frame = +3
Query: 16 EVKKKTEEEKKKEEEKKKEEEKKK 39
E K++ ++E++K+EEKK+E EKK+
Sbjct: 156 EKKEEPKKEEEKKEEKKEEGEKKE 227
>AW688848 homologue to PIR|T46027|T4 hypothetical protein T10K17.260 -
Arabidopsis thaliana, partial (10%)
Length = 661
Score = 33.5 bits (75), Expect = 0.040
Identities = 16/29 (55%), Positives = 21/29 (72%)
Frame = +1
Query: 10 TILSVEEVKKKTEEEKKKEEEKKKEEEKK 38
T L EE K+K EEE++KE ++ KE EKK
Sbjct: 247 TKLLEEEEKEKREEEERKERKRAKEREKK 333
>AW586908 weakly similar to GP|2104814|emb| pleiotropic effects on cellular
differentiation and slug behaviour {Dictyostelium
discoideum}, partial (8%)
Length = 668
Score = 33.5 bits (75), Expect = 0.040
Identities = 32/122 (26%), Positives = 39/122 (31%), Gaps = 14/122 (11%)
Frame = -3
Query: 50 GSCNSCCKTNCNGKCEKCTCGSAKCDGIQCVTICFKCENPKSCCECKPIIKS-CCNNKKC 108
G C CC C C C C C C + F C C P+ CCNN C
Sbjct: 633 GCCCCCCCCCCCNFCNNCNC----C----CWSEMFCGTV*DICGCCGPLCNMLCCNN--C 484
Query: 109 DDGCTPKKPPSPKVQQCPQWCTCSRCYGYAPYQPYC-------------NPCPPPNYMVC 155
++ C C +C C RC A C NP P + C
Sbjct: 483 NEDC------------CN*FCCCCRCKRPACCWSICCWNCESCGKDVIINPFLPADCKFC 340
Query: 156 YD 157
+D
Sbjct: 339 WD 334
Score = 30.8 bits (68), Expect = 0.26
Identities = 22/77 (28%), Positives = 28/77 (35%), Gaps = 16/77 (20%)
Frame = -3
Query: 52 CNSCCKTNCNGKCEKCTCGSAKCDGIQCV--TICFKCE--------NP------KSCCEC 95
CN C NCN C C +C C C+ CE NP K C +C
Sbjct: 510 CNMLCCNNCNEDCCN*FCCCCRCKRPACCWSICCWNCESCGKDVIINPFLPADCKFCWDC 331
Query: 96 KPIIKSCCNNKKCDDGC 112
+CC + + GC
Sbjct: 330 ILPESNCC*S*E*VTGC 280
Score = 30.0 bits (66), Expect = 0.45
Identities = 22/67 (32%), Positives = 30/67 (43%), Gaps = 3/67 (4%)
Frame = -3
Query: 52 CNSCCKTNCNGKCEKCTCGSAKCDGIQCVTICFKCENPKSCCE---CKPIIKSCCNNKKC 108
CN+C + CN C C C C C +IC C N +SC + P + + C K C
Sbjct: 495 CNNCNEDCCN*FCCCCRCKRPAC----CWSIC--CWNCESCGKDVIINPFLPADC--KFC 340
Query: 109 DDGCTPK 115
D P+
Sbjct: 339 WDCILPE 319
>TC88253 weakly similar to GP|15810597|gb|AAL07186.1 unknown protein
{Arabidopsis thaliana}, partial (51%)
Length = 1122
Score = 33.5 bits (75), Expect = 0.040
Identities = 17/38 (44%), Positives = 28/38 (72%), Gaps = 2/38 (5%)
Frame = +3
Query: 4 KKFNCVTILSVEEVKKKTEEEKKKEE--EKKKEEEKKK 39
+ F+ ++ S+ E+ ++ +EE KKEE E+KKEEEKK+
Sbjct: 66 QNFSSISHCSIIEMGEENKEEGKKEETKEEKKEEEKKE 179
Score = 32.7 bits (73), Expect = 0.069
Identities = 17/32 (53%), Positives = 25/32 (78%), Gaps = 2/32 (6%)
Frame = +3
Query: 13 SVEEVKKKTEEEKKKEEEKK--KEEEKKKMME 42
S+ + ++K EEEKK+EE+K+ KEEEKK+ E
Sbjct: 687 SIVKKEEKKEEEKKEEEKKEEVKEEEKKESEE 782
Score = 32.7 bits (73), Expect = 0.069
Identities = 17/36 (47%), Positives = 27/36 (74%), Gaps = 7/36 (19%)
Frame = +3
Query: 14 VEEVKKKTEE-------EKKKEEEKKKEEEKKKMME 42
V+EV K+T++ E+KKEEEKK+EE+K+++ E
Sbjct: 651 VDEVFKRTKKQASIVKKEEKKEEEKKEEEKKEEVKE 758
Score = 32.0 bits (71), Expect = 0.12
Identities = 15/43 (34%), Positives = 26/43 (59%)
Frame = +3
Query: 15 EEVKKKTEEEKKKEEEKKKEEEKKKMMEACRTVLFGSCNSCCK 57
EE KK+ +E+KKEEEKK+E++ ++ + C +C +
Sbjct: 123 EEGKKEETKEEKKEEEKKEEKKDEEQPPEIVLKVDMHCEACAR 251
Score = 31.2 bits (69), Expect = 0.20
Identities = 18/39 (46%), Positives = 26/39 (66%), Gaps = 2/39 (5%)
Frame = +3
Query: 3 KKKFNCVTILSVEEVKKKTEEEKK--KEEEKKKEEEKKK 39
KK+ + V +E +KK EE+K+ KEEEKK+ EE+ K
Sbjct: 675 KKQASIVKKEEKKEEEKKEEEKKEEVKEEEKKESEEENK 791
>BG644345 weakly similar to GP|19697333|gb putative protein potential
transcriptional repressor Not4hp - Mus musculus, partial
(7%)
Length = 635
Score = 33.1 bits (74), Expect = 0.053
Identities = 24/81 (29%), Positives = 27/81 (32%), Gaps = 18/81 (22%)
Frame = -1
Query: 52 CNSCCKTNCNGKCEKCTCGSAKCDGIQCVTICFKCENPKSCCECKPII-----KSCC--- 103
C+SCC CN C C C C C C N CC C+ + CC
Sbjct: 446 CSSCCCCCCNCNCCCC*SCCCCCC*SCCCCCCCCC*N*SCCCCCEKGVI*ENGDGCCWPG 267
Query: 104 --NNKKC--------DDGCTP 114
N C DD C P
Sbjct: 266 VWNGDACCCPGVWNGDDCCCP 204
>TC80931 similar to GP|14190783|gb|AAF65166.2 putative phloem transcription
factor M1 {Apium graveolens}, partial (67%)
Length = 1193
Score = 33.1 bits (74), Expect = 0.053
Identities = 21/63 (33%), Positives = 22/63 (34%), Gaps = 9/63 (14%)
Frame = -3
Query: 50 GSCNSCCKTNCNGKC------EKCTC---GSAKCDGIQCVTICFKCENPKSCCECKPIIK 100
GSC C NC C E C C GS C C C E+ CC C
Sbjct: 774 GSC*CCSCCNCGSCCCCCCS*ESCCC*S*GSCCCCCWSCGICC*IWESC*FCCSCGSCCC 595
Query: 101 SCC 103
CC
Sbjct: 594 CCC 586
>TC92466 similar to GP|11141605|gb|AAG32022.1 LEUNIG {Arabidopsis thaliana},
partial (14%)
Length = 632
Score = 32.7 bits (73), Expect = 0.069
Identities = 17/49 (34%), Positives = 18/49 (36%)
Frame = -1
Query: 55 CCKTNCNGKCEKCTCGSAKCDGIQCVTICFKCENPKSCCECKPIIKSCC 103
CC C C C CG C C C C +SCC C CC
Sbjct: 566 CCCCGCG--CCGCDCGCC*C--CCCCCCCCACLCIRSCCICMCCCCCCC 432
>TC80312 weakly similar to GP|19698847|gb|AAL91159.1 unknown protein
{Arabidopsis thaliana}, partial (20%)
Length = 1595
Score = 32.7 bits (73), Expect = 0.069
Identities = 15/41 (36%), Positives = 26/41 (62%)
Frame = +2
Query: 9 VTILSVEEVKKKTEEEKKKEEEKKKEEEKKKMMEACRTVLF 49
V + S E+VK K +KKK+ ++++ KK + AC ++LF
Sbjct: 776 VPLKSPEKVKAKPSRKKKKKVARERDLFKKNYIHACESLLF 898
>TC81969 similar to GP|6523547|emb|CAB62280.1 hydroxyproline-rich
glycoprotein DZ-HRGP {Volvox carteri f. nagariensis},
partial (11%)
Length = 556
Score = 32.7 bits (73), Expect = 0.069
Identities = 19/48 (39%), Positives = 29/48 (59%), Gaps = 5/48 (10%)
Frame = +3
Query: 18 KKKTEEEKKKEEEKKKEEEKKKM----MEACRTVLFGSCNSC-CKTNC 60
K K + K++EEE+K+EEEKKK M+ +L ++C C T+C
Sbjct: 294 KAKDGKTKEEEEEEKEEEEKKKKGSANMKRPENILENYKHACVCSTSC 437
>TC80831 similar to GP|18542939|gb|AAK00428.2 Unknown protein {Oryza
sativa}, partial (7%)
Length = 1039
Score = 32.3 bits (72), Expect = 0.090
Identities = 14/24 (58%), Positives = 18/24 (74%)
Frame = +2
Query: 15 EEVKKKTEEEKKKEEEKKKEEEKK 38
EE K+K EEE++KE + KE EKK
Sbjct: 137 EEEKEKREEEERKERRRTKEREKK 208
>TC77769 similar to PIR|T06397|T06397 isoprenylated protein FP6 - soybean
(fragment), partial (41%)
Length = 1132
Score = 32.3 bits (72), Expect = 0.090
Identities = 15/41 (36%), Positives = 28/41 (67%)
Frame = +1
Query: 11 ILSVEEVKKKTEEEKKKEEEKKKEEEKKKMMEACRTVLFGS 51
ILSV K++ +EE KK+++KK++++K M +L+G+
Sbjct: 628 ILSVGPAKEEKKEEPKKDDKKKEDDKKDSTMIINPFMLYGT 750
>TC78900 homologue to SP|P51819|HS83_PHANI Heat shock protein 83. [Violet
Japanese morning glory] {Pharbitis nil}, partial (96%)
Length = 2432
Score = 32.3 bits (72), Expect = 0.090
Identities = 16/31 (51%), Positives = 22/31 (70%), Gaps = 1/31 (3%)
Frame = +1
Query: 18 KKKTEEEKKKEEEKKKEEEKKKMME-ACRTV 47
+K + +++ EEEKKK EEKKK E CRT+
Sbjct: 1573 RKGLKLDEESEEEKKKREEKKKSFEDLCRTM 1665
>TC77767 weakly similar to GP|22002137|gb|AAM88621.1 hypothetical protein
{Oryza sativa (japonica cultivar-group)}, partial (36%)
Length = 631
Score = 32.3 bits (72), Expect = 0.090
Identities = 15/41 (36%), Positives = 28/41 (67%)
Frame = +2
Query: 11 ILSVEEVKKKTEEEKKKEEEKKKEEEKKKMMEACRTVLFGS 51
ILSV K++ +EE KK+++KK++++K M +L+G+
Sbjct: 257 ILSVGPAKEEKKEEPKKDDKKKEDDKKDSTMIINPFMLYGT 379
>TC79752 similar to GP|11994670|dbj|BAB02898. gene_id:K7P8.17~unknown protein
{Arabidopsis thaliana}, partial (2%)
Length = 2137
Score = 32.0 bits (71), Expect = 0.12
Identities = 24/75 (32%), Positives = 31/75 (41%), Gaps = 9/75 (12%)
Frame = -1
Query: 55 CCKTNCNGKCEKCTCGSAKCDGI--QC-------VTICFKCENPKSCCECKPIIKSCCNN 105
CCK +GKC C+C C G C V C + + CC C P + C +
Sbjct: 2104 CCKATSSGKC--CSCSCC*CSGSWHDC*DRFGFNVDCCLRGKG--WCCRCAP--ELCE*D 1943
Query: 106 KKCDDGCTPKKPPSP 120
K D C + PSP
Sbjct: 1942 AKLADNC---RGPSP 1907
>BG645312 similar to GP|16549312|dbj unnamed protein product {Homo sapiens},
partial (5%)
Length = 188
Score = 32.0 bits (71), Expect = 0.12
Identities = 13/23 (56%), Positives = 19/23 (82%)
Frame = -3
Query: 18 KKKTEEEKKKEEEKKKEEEKKKM 40
KKK ++KKK++EKKK E +KK+
Sbjct: 102 KKKKRKKKKKKKEKKKRERRKKV 34
Score = 30.4 bits (67), Expect = 0.34
Identities = 13/28 (46%), Positives = 21/28 (74%)
Frame = -3
Query: 11 ILSVEEVKKKTEEEKKKEEEKKKEEEKK 38
I + + KKK +++KKK+E+KK+E KK
Sbjct: 120 ITNKNKKKKKRKKKKKKKEKKKRERRKK 37
Score = 27.7 bits (60), Expect = 2.2
Identities = 10/22 (45%), Positives = 19/22 (85%)
Frame = -3
Query: 18 KKKTEEEKKKEEEKKKEEEKKK 39
KKK + +KKK++++KK+ E++K
Sbjct: 105 KKKKKRKKKKKKKEKKKRERRK 40
>AW774293 similar to GP|13881624|gb PPE family protein {Mycobacterium
tuberculosis CDC1551}, partial (1%)
Length = 597
Score = 31.2 bits (69), Expect = 0.20
Identities = 26/92 (28%), Positives = 33/92 (35%), Gaps = 11/92 (11%)
Frame = -1
Query: 86 CENPKSCCECK---PIIKSCCN------NKKCDDGCTPKKPPSPKVQQCPQWCTC--SRC 134
C + K CC C P SCC+ + C D C KP K + P++ C S C
Sbjct: 588 CPSMKCCCSCXCQLPKCSSCCSCINFSLSINCPDCCW--KPSCIKCCRLPKFTDCFGSTC 415
Query: 135 YGYAPYQPYCNPCPPPNYMVCYDPNPEPCSIM 166
C CP + CY P M
Sbjct: 414 CTKCSMPGCC--CPKCSLPCCYPKCSRPSCCM 325
Score = 30.8 bits (68), Expect = 0.26
Identities = 16/51 (31%), Positives = 19/51 (36%), Gaps = 6/51 (11%)
Frame = -1
Query: 51 SCNSCCK----TNCNGK--CEKCTCGSAKCDGIQCVTICFKCENPKSCCEC 95
SC CC+ T+C G C KC+ C KC P C C
Sbjct: 471 SCIKCCRLPKFTDCFGSTCCTKCSMPGCCCPKCSLPCCYPKCSRPSCCMSC 319
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.319 0.135 0.478
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 10,290,055
Number of Sequences: 36976
Number of extensions: 273954
Number of successful extensions: 3673
Number of sequences better than 10.0: 259
Number of HSP's better than 10.0 without gapping: 2663
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3288
length of query: 166
length of database: 9,014,727
effective HSP length: 89
effective length of query: 77
effective length of database: 5,723,863
effective search space: 440737451
effective search space used: 440737451
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 54 (25.4 bits)
Medicago: description of AC146569.7