BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146567.12 + phase: 0 /pseudo
(307 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC93531 similar to PIR|E86271|E86271 protein F16A14.4 [imported]... 374 e-107
TC79677 similar to GP|13877561|gb|AAK43858.1 Unknown protein {Ar... 77 1e-14
TC84102 similar to GP|15451078|gb|AAK96810.1 Unknown protein {Ar... 49 2e-06
TC80671 similar to GP|22093633|dbj|BAC06928. hypothetical protei... 42 4e-04
TC79420 weakly similar to GP|1354849|gb|AAB02006.1| epoxide hydr... 41 5e-04
TC93140 similar to GP|2262100|gb|AAB63608.1| unknown protein {Ar... 40 9e-04
TC88153 similar to GP|1354849|gb|AAB02006.1| epoxide hydrolase {... 40 0.001
TC78414 similar to GP|1354849|gb|AAB02006.1| epoxide hydrolase {... 39 0.003
TC79861 similar to PIR|T01316|T01316 epoxide hydrolase homolog T... 35 0.047
TC78177 similar to PIR|T07145|T07145 epoxide hydrolase homolog -... 35 0.047
TC77584 similar to PIR|T07145|T07145 epoxide hydrolase homolog -... 35 0.047
TC77524 similar to PIR|T07145|T07145 epoxide hydrolase homolog -... 34 0.062
TC77525 similar to PIR|T07145|T07145 epoxide hydrolase homolog -... 34 0.062
TC79568 weakly similar to GP|21689747|gb|AAM67517.1 unknown prot... 34 0.062
TC92746 similar to PIR|T07145|T07145 epoxide hydrolase homolog -... 34 0.081
TC90757 similar to GP|17528996|gb|AAL38708.1 unknown protein {Ar... 33 0.11
TC88515 weakly similar to PIR|T45731|T45731 epoxide hydrolase-li... 33 0.11
AJ500952 weakly similar to GP|11994705|db hydrolase-like protein... 33 0.18
TC78176 similar to PIR|T07145|T07145 epoxide hydrolase homolog -... 33 0.18
BF647289 weakly similar to PIR|T01316|T013 epoxide hydrolase hom... 32 0.24
>TC93531 similar to PIR|E86271|E86271 protein F16A14.4 [imported] -
Arabidopsis thaliana, partial (19%)
Length = 637
Score = 374 bits (959), Expect(2) = e-107
Identities = 183/184 (99%), Positives = 184/184 (99%)
Frame = +3
Query: 17 ETDADSFPSFLPKDVNRIKDPFARTLATRIQRLPVSVKFSENPIMSSCVKPLVQNKENPI 76
ETDADSFPSFLPKDVNRIKDPFARTLATRIQRLPVSVKFSENPIMSSCVKPLVQNKENPI
Sbjct: 84 ETDADSFPSFLPKDVNRIKDPFARTLATRIQRLPVSVKFSENPIMSSCVKPLVQNKENPI 263
Query: 77 VLLHGFDSSCLEWRYTYPLLEEAGIETWAIDILGWGFSDLEKLPSCDVVSKREHFYQFWK 136
VLLHGFDSSCLEWRYTYPLLEEAGIETWAIDILGWGFSDLEKLPSCDVVSKREHFYQFWK
Sbjct: 264 VLLHGFDSSCLEWRYTYPLLEEAGIETWAIDILGWGFSDLEKLPSCDVVSKREHFYQFWK 443
Query: 137 SYIKKPMILVGPSLGSAVAIDFAINYPEAVEKLVLIDASVYTEGTGNLATLPRAAAYAGV 196
SYIKKPMILVGPSLGSAVAIDFAINYPEAVEKLVLIDASVYTEGTGNLATLP+AAAYAGV
Sbjct: 444 SYIKKPMILVGPSLGSAVAIDFAINYPEAVEKLVLIDASVYTEGTGNLATLPKAAAYAGV 623
Query: 197 YLLK 200
YLLK
Sbjct: 624 YLLK 635
Score = 33.5 bits (75), Expect(2) = e-107
Identities = 16/16 (100%), Positives = 16/16 (100%)
Frame = +2
Query: 1 MVALFSFALPIFKVVA 16
MVALFSFALPIFKVVA
Sbjct: 35 MVALFSFALPIFKVVA 82
>TC79677 similar to GP|13877561|gb|AAK43858.1 Unknown protein {Arabidopsis
thaliana}, partial (75%)
Length = 1150
Score = 76.6 bits (187), Expect = 1e-14
Identities = 41/108 (37%), Positives = 66/108 (60%)
Frame = +3
Query: 68 LVQNKENPIVLLHGFDSSCLEWRYTYPLLEEAGIETWAIDILGWGFSDLEKLPSCDVVSK 127
+VQ + P+VL+HGF +S WRY P L + + +A+D+LG+G+SD + L D +
Sbjct: 450 VVQGEGPPLVLIHGFGASAFHWRYNIPELAKKH-KVYALDLLGFGWSD-KALVDYDAMVW 623
Query: 128 REHFYQFWKSYIKKPMILVGPSLGSAVAIDFAINYPEAVEKLVLIDAS 175
R+ F K +K+P +LVG SLG A+ A PE+V +VL++++
Sbjct: 624 RDQVVDFMKEIVKEPAVLVGNSLGGFTALIAAAGLPESVTGVVLLNSA 767
>TC84102 similar to GP|15451078|gb|AAK96810.1 Unknown protein {Arabidopsis
thaliana}, partial (55%)
Length = 771
Score = 49.3 bits (116), Expect = 2e-06
Identities = 40/143 (27%), Positives = 66/143 (45%), Gaps = 5/143 (3%)
Frame = +2
Query: 43 ATRIQRLPVSVKFSENPIMSSCVKPLVQNKENPIVLLHGFDSSCLEWRYTYPLLEEAGIE 102
++R + LP SV P+ + LV K VL+HG W T LLEE G++
Sbjct: 254 SSRRRTLPDSV-----PVNKQDPELLVNIKTKKFVLIHGEGFGAWCWYKTVALLEEVGLQ 418
Query: 103 TWAIDILGWGFSDLEKLPSCDVVSKREHFYQFWKSYIK-----KPMILVGPSLGSAVAID 157
A+D+ G G L + V+ + + Y++ + +ILVG S+G A
Sbjct: 419 PVALDLTGSGID----LTDSNNVTTLAEYSKPLTVYLENLPEDEKVILVGHSIGGACISY 586
Query: 158 FAINYPEAVEKLVLIDASVYTEG 180
+YP + K + + A++ T+G
Sbjct: 587 AXEHYPHKISKAIFLCATMVTDG 655
>TC80671 similar to GP|22093633|dbj|BAC06928. hypothetical protein~similar
to Arabidopsis thaliana chromosome5 At5g19850, partial
(70%)
Length = 1101
Score = 41.6 bits (96), Expect = 4e-04
Identities = 30/116 (25%), Positives = 57/116 (48%), Gaps = 10/116 (8%)
Frame = +2
Query: 71 NKENPIVLLHGFDSSCLEWRYTYPLLEEAGIETWAIDILGWGFSDLEKLPSCDVVSKREH 130
N +VL+HGF ++ WR P L ++ ++ID++G+G+SD P+ + +
Sbjct: 236 NNGPALVLVHGFGANSDHWRKNIPDLAKSH-RVYSIDLIGYGYSD---KPNPRQIGD-DS 400
Query: 131 FYQF--WKS--------YIKKPMILVGPSLGSAVAIDFAINYPEAVEKLVLIDASV 176
FY F W + +K + S+G V + AI P+ + ++L++ S+
Sbjct: 401 FYTFDTWAAQLNEFCLDVVKDEAFFICNSIGGVVGLQAAITEPQICKGILLLNISL 568
>TC79420 weakly similar to GP|1354849|gb|AAB02006.1| epoxide hydrolase
{Nicotiana tabacum}, partial (90%)
Length = 1433
Score = 41.2 bits (95), Expect = 5e-04
Identities = 40/143 (27%), Positives = 55/143 (37%), Gaps = 3/143 (2%)
Frame = +2
Query: 74 NPIVLLHGFDSSCLEWRYTYPLLEEAGIETWAIDILGWGFSDLEKLPSCDVVSK-REHFY 132
N +V LHGF WR+ L AG A D G+G SD P +
Sbjct: 407 NVVVFLHGFPEIWYSWRHQMLALAGAGFRAIAPDYRGYGLSDSPPEPEKTTFTHLLNDLL 586
Query: 133 QFWKSYIKKPMILVGPSLGSAVAIDFAINYPEAVEKLVLIDASVYTEGTGNL-ATLPRAA 191
Q + + LVG G A F+I +PE V ++ + G L LP
Sbjct: 587 QIIDALAISKVFLVGKDFGGPPAYLFSILHPERVLGVITLGVPYVPPGPSMLHKYLPE-- 760
Query: 192 AYAGVYLLK-SVPLRVYANYLSF 213
G Y+L+ P R A++ F
Sbjct: 761 ---GFYILRWKEPGRAEADFGRF 820
>TC93140 similar to GP|2262100|gb|AAB63608.1| unknown protein {Arabidopsis
thaliana}, partial (63%)
Length = 857
Score = 40.4 bits (93), Expect = 9e-04
Identities = 31/111 (27%), Positives = 54/111 (47%), Gaps = 5/111 (4%)
Frame = +2
Query: 76 IVLLHGFDSSCLEWRYTYPLLEEAGIETWAIDILGWGFSDLEKLPSCDVVSKRE-----H 130
+V++HG+ +S + + L AID LGWG S +C + E
Sbjct: 266 LVMVHGYAASQGFFFRNFDALASR-FRVIAIDQLGWGGSSRPDF-TCKSTEETEAWFIDS 439
Query: 131 FYQFWKSYIKKPMILVGPSLGSAVAIDFAINYPEAVEKLVLIDASVYTEGT 181
F ++ K+ IL+G S G +A +A+ +PE V+ LVL+ ++ ++ T
Sbjct: 440 FEEWRKAKNLSNFILLGHSFGGYIASKYALKHPEHVQHLVLVGSAGFSSET 592
>TC88153 similar to GP|1354849|gb|AAB02006.1| epoxide hydrolase {Nicotiana
tabacum}, partial (82%)
Length = 1143
Score = 39.7 bits (91), Expect = 0.001
Identities = 27/101 (26%), Positives = 40/101 (38%), Gaps = 1/101 (0%)
Frame = +1
Query: 73 ENPIVLLHGFDSSCLEWRYTYPLLEEAGIETWAIDILGWGFSDLEKLP-SCDVVSKREHF 131
E +V LHGF WRY + AG A D G+G SD P ++ +
Sbjct: 94 EKVVVFLHGFPEIWYTWRYQMIAVANAGYRAIAFDFRGYGLSDHPAEPEKATIMDLVDDV 273
Query: 132 YQFWKSYIKKPMILVGPSLGSAVAIDFAINYPEAVEKLVLI 172
+ IL+G G+ A A +PE V ++ +
Sbjct: 274 KDLLDTLGISNAILIGKDFGAIPAYLVAAVHPEKVASVITL 396
>TC78414 similar to GP|1354849|gb|AAB02006.1| epoxide hydrolase {Nicotiana
tabacum}, partial (68%)
Length = 900
Score = 38.9 bits (89), Expect = 0.003
Identities = 27/94 (28%), Positives = 37/94 (38%), Gaps = 1/94 (1%)
Frame = +2
Query: 74 NPIVLLHGFDSSCLEWRYTYPLLEEAGIETWAIDILGWGFSDLEKLPSCDVVSK-REHFY 132
N +V LHGF WR+ + AG A D G+G SD P +
Sbjct: 161 NVVVFLHGFPEIWYSWRHQMIAVAGAGFRAIAFDYRGYGLSDSPPEPEKTTFTHLLNDLL 340
Query: 133 QFWKSYIKKPMILVGPSLGSAVAIDFAINYPEAV 166
+ + LVG G+ A F+I +PE V
Sbjct: 341 AILDALSLSKVFLVGKDFGARPAYLFSILHPERV 442
>TC79861 similar to PIR|T01316|T01316 epoxide hydrolase homolog T14P8.15 -
Arabidopsis thaliana, partial (86%)
Length = 1162
Score = 34.7 bits (78), Expect = 0.047
Identities = 29/100 (29%), Positives = 41/100 (41%), Gaps = 5/100 (5%)
Frame = +1
Query: 76 IVLLHGFDSSCLEWRYTYPLLEEAGIETWAIDILGWGFSDLEKLPSCDVVSKREH----- 130
I+ LHGF WR+ L G A D+ G+G +D PS S H
Sbjct: 91 IIFLHGFPELWYSWRHQLLSLSALGYRAVAPDLRGYGDTDSPSSPS----SYTAHHIVGD 258
Query: 131 FYQFWKSYIKKPMILVGPSLGSAVAIDFAINYPEAVEKLV 170
+ + LVG G+A+A F + P+ V+ LV
Sbjct: 259 LVGLLDALNVDRVFLVGHDWGAAMAWYFCLLKPDRVKALV 378
>TC78177 similar to PIR|T07145|T07145 epoxide hydrolase homolog - soybean,
partial (89%)
Length = 1037
Score = 34.7 bits (78), Expect = 0.047
Identities = 18/48 (37%), Positives = 25/48 (51%), Gaps = 1/48 (2%)
Frame = +2
Query: 70 QNKENPIVL-LHGFDSSCLEWRYTYPLLEEAGIETWAIDILGWGFSDL 116
+ KE P+VL LHGF WR+ L G A D+ G+G +D+
Sbjct: 89 KGKEGPVVLFLHGFPELWYSWRHQIAALGSLGYRAVAPDLRGYGDTDV 232
>TC77584 similar to PIR|T07145|T07145 epoxide hydrolase homolog - soybean,
partial (70%)
Length = 819
Score = 34.7 bits (78), Expect = 0.047
Identities = 18/47 (38%), Positives = 24/47 (50%), Gaps = 1/47 (2%)
Frame = +2
Query: 70 QNKENPIVL-LHGFDSSCLEWRYTYPLLEEAGIETWAIDILGWGFSD 115
+ KE P+VL LHGF WR+ L G A D+ G+G +D
Sbjct: 137 KGKEGPVVLFLHGFPELWYSWRHQIVALSSLGYRAVAPDLRGYGDTD 277
>TC77524 similar to PIR|T07145|T07145 epoxide hydrolase homolog - soybean,
partial (81%)
Length = 1262
Score = 34.3 bits (77), Expect = 0.062
Identities = 18/47 (38%), Positives = 24/47 (50%), Gaps = 1/47 (2%)
Frame = +2
Query: 70 QNKENPIVL-LHGFDSSCLEWRYTYPLLEEAGIETWAIDILGWGFSD 115
+ KE P+VL LHGF WR+ L G A D+ G+G +D
Sbjct: 74 KGKEGPVVLFLHGFPELWYSWRHQIAALGSLGYRAVAPDLRGYGDTD 214
>TC77525 similar to PIR|T07145|T07145 epoxide hydrolase homolog - soybean,
partial (88%)
Length = 1457
Score = 34.3 bits (77), Expect = 0.062
Identities = 18/47 (38%), Positives = 24/47 (50%), Gaps = 1/47 (2%)
Frame = +2
Query: 70 QNKENPIVL-LHGFDSSCLEWRYTYPLLEEAGIETWAIDILGWGFSD 115
+ KE P+VL LHGF WR+ L G A D+ G+G +D
Sbjct: 164 KGKEGPVVLFLHGFPELWYSWRHQIAALGSLGYRAVAPDLRGYGDTD 304
>TC79568 weakly similar to GP|21689747|gb|AAM67517.1 unknown protein
{Arabidopsis thaliana}, partial (25%)
Length = 1192
Score = 34.3 bits (77), Expect = 0.062
Identities = 17/49 (34%), Positives = 24/49 (48%)
Frame = +3
Query: 67 PLVQNKENPIVLLHGFDSSCLEWRYTYPLLEEAGIETWAIDILGWGFSD 115
P + N +++LHG S +R L G+ AID+ G GFSD
Sbjct: 372 PTIPNPSETVLILHGQALSSYSYRNLIQSLSTQGVRVIAIDLPGSGFSD 518
>TC92746 similar to PIR|T07145|T07145 epoxide hydrolase homolog - soybean,
partial (44%)
Length = 523
Score = 33.9 bits (76), Expect = 0.081
Identities = 18/45 (40%), Positives = 23/45 (51%), Gaps = 1/45 (2%)
Frame = +2
Query: 72 KENPIVL-LHGFDSSCLEWRYTYPLLEEAGIETWAIDILGWGFSD 115
KE P+VL LHGF WR+ L G A D+ G+G +D
Sbjct: 2 KEGPVVLFLHGFPELWYSWRHQISALGSLGYRAVAPDLRGYGDTD 136
>TC90757 similar to GP|17528996|gb|AAL38708.1 unknown protein {Arabidopsis
thaliana}, partial (41%)
Length = 717
Score = 33.5 bits (75), Expect = 0.11
Identities = 16/45 (35%), Positives = 24/45 (52%)
Frame = +3
Query: 71 NKENPIVLLHGFDSSCLEWRYTYPLLEEAGIETWAIDILGWGFSD 115
++ IV LHG + +R L +AG +A D +G+GFSD
Sbjct: 387 SRRGTIVFLHGAPTQSFSYRVVMSELGDAGFHCFAPDWIGFGFSD 521
>TC88515 weakly similar to PIR|T45731|T45731 epoxide hydrolase-like protein
- Arabidopsis thaliana, partial (51%)
Length = 771
Score = 33.5 bits (75), Expect = 0.11
Identities = 27/106 (25%), Positives = 47/106 (43%), Gaps = 9/106 (8%)
Frame = +2
Query: 76 IVLLHGFDSSCLEWRYTYPLLEEAGIETWAIDILGWGFSD---------LEKLPSCDVVS 126
++LLHGF WR+ L + G A D+ G+G SD L + D++
Sbjct: 227 VLLLHGFPEIWYSWRHQLNYLAQNGYHAVAPDLRGYGDSDSPINSDSYTLHHIVG-DLIG 403
Query: 127 KREHFYQFWKSYIKKPMILVGPSLGSAVAIDFAINYPEAVEKLVLI 172
+HF + K+Y+ VG G+ + ++ P+ V+ V +
Sbjct: 404 LLDHFGEH-KAYV------VGSDWGANIGWHLSLFRPDRVKGFVAL 520
>AJ500952 weakly similar to GP|11994705|db hydrolase-like protein
{Arabidopsis thaliana}, partial (38%)
Length = 663
Score = 32.7 bits (73), Expect = 0.18
Identities = 28/107 (26%), Positives = 45/107 (41%), Gaps = 7/107 (6%)
Frame = +2
Query: 76 IVLLHGFDSSCLEWRYTYPLLEEAGIETWAIDILGWGFSDLEKLPSCDVVSKREHFYQFW 135
IVL HGF + W PLL E +++ + W FS S +K + +
Sbjct: 146 IVLCHGFGTDQSTWNKIVPLL----AENYSLVLFDWPFSGAITDKSLYNHAKYNSYEPYA 313
Query: 136 KSYIK-------KPMILVGPSLGSAVAIDFAINYPEAVEKLVLIDAS 175
I K + VG S+ + + +I PE +++VL+ AS
Sbjct: 314 DELITLMDELDLKCITFVGHSMAAMIGCIASIKKPELFKRIVLLCAS 454
>TC78176 similar to PIR|T07145|T07145 epoxide hydrolase homolog - soybean,
partial (79%)
Length = 882
Score = 32.7 bits (73), Expect = 0.18
Identities = 17/44 (38%), Positives = 22/44 (49%), Gaps = 1/44 (2%)
Frame = +3
Query: 70 QNKENPIVL-LHGFDSSCLEWRYTYPLLEEAGIETWAIDILGWG 112
+ KE P+VL LHGF WR+ L G A D+ G+G
Sbjct: 120 KGKEGPVVLFLHGFPELWYSWRHQIAALGSLGYRAVAPDLRGYG 251
>BF647289 weakly similar to PIR|T01316|T013 epoxide hydrolase homolog
T14P8.15 - Arabidopsis thaliana, partial (24%)
Length = 682
Score = 32.3 bits (72), Expect = 0.24
Identities = 15/40 (37%), Positives = 21/40 (52%)
Frame = +2
Query: 76 IVLLHGFDSSCLEWRYTYPLLEEAGIETWAIDILGWGFSD 115
++LLHGF WR+ L + G A D+ G+G SD
Sbjct: 107 VLLLHGFPEIWYSWRHQLNYLAQNGYHAVAPDLRGYGDSD 226
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.335 0.145 0.460
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 9,507,057
Number of Sequences: 36976
Number of extensions: 136667
Number of successful extensions: 1072
Number of sequences better than 10.0: 52
Number of HSP's better than 10.0 without gapping: 1062
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1065
length of query: 307
length of database: 9,014,727
effective HSP length: 96
effective length of query: 211
effective length of database: 5,465,031
effective search space: 1153121541
effective search space used: 1153121541
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 39 (21.6 bits)
S2: 58 (26.9 bits)
Medicago: description of AC146567.12