BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146557.6 - phase: 0 /pseudo
(487 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC80324 similar to PIR|T04993|T04993 probable aspartate--tRNA li... 270 9e-73
TC92703 similar to PIR|T04993|T04993 probable aspartate--tRNA li... 264 5e-71
BI264098 similar to GP|13872951|dbj putative aspartate--tRNA lig... 244 7e-65
CB065281 similar to SP|Q51422|SYD_ Aspartyl-tRNA synthetase (EC ... 103 2e-22
TC87396 similar to GP|21537130|gb|AAM61471.1 unknown {Arabidopsi... 30 2.1
TC80265 similar to GP|15450695|gb|AAK96619.1 AT5g13640/MSH12_10 ... 29 4.6
TC91124 weakly similar to GP|15809820|gb|AAL06838.1 At1g29690/F1... 28 7.9
TC79892 similar to GP|15010706|gb|AAK74012.1 At2g25350/F13B15.1 ... 28 7.9
BF633115 weakly similar to GP|15809820|gb At1g29690/F15D2_24 {Ar... 28 7.9
>TC80324 similar to PIR|T04993|T04993 probable aspartate--tRNA ligase (EC
6.1.1.12) T16L1.250 - Arabidopsis thaliana, partial
(27%)
Length = 843
Score = 270 bits (690), Expect = 9e-73
Identities = 147/238 (61%), Positives = 177/238 (73%), Gaps = 13/238 (5%)
Frame = +1
Query: 1 MWSLFLKALSLISIRRSPSL----RKTKIPLPYYEKLKPKTKKAITF----KKPKP---- 48
M SLFL+AL L ++RR+PS+ R +K + + +T +++ ++P P
Sbjct: 97 MSSLFLRALPLTTLRRTPSIFFLSRLSKTIPTLISRTRTRTLSSVSASIQQQQPLPSETE 276
Query: 49 -LNQSLQWISRTHHCGELSSNDVGKTVRLCGWAELHRSLGGVAFLILRDQTGIIQVKTRL 107
LN+SLQW+SRTHHCGELSSNDVGKTVRLCGW LHR GG+ FL LRD TGI+QV T
Sbjct: 277 TLNESLQWVSRTHHCGELSSNDVGKTVRLCGWVALHRLHGGLTFLNLRDHTGIVQVTTLP 456
Query: 108 DEFPVAHSANKNLRREYVAAIQGVVRSRSTQSINYEMKTGFIEVAANEVQELNSRNAKLT 167
DEFPVAHSA NLR EYV AIQG+VR R QSIN ++KTGFIE+AAN+VQ LNS NAKL
Sbjct: 457 DEFPVAHSAINNLRLEYVVAIQGIVRPRPNQSINNKIKTGFIEIAANDVQLLNSVNAKLP 636
Query: 168 LIPELNSDDAEGSPNEEIRLRYRCLDLRKQQMNSNILLRHNVVKLIRRYLEDIHGFVE 225
+ +D A+ S EE+RLRYR LDLR+QQMNSN+LLRHNVVKL+RRYLEDIHGFVE
Sbjct: 637 FL-VTTTDHAKDSLREEVRLRYRYLDLRRQQMNSNMLLRHNVVKLMRRYLEDIHGFVE 807
>TC92703 similar to PIR|T04993|T04993 probable aspartate--tRNA ligase (EC
6.1.1.12) T16L1.250 - Arabidopsis thaliana, partial
(30%)
Length = 694
Score = 264 bits (675), Expect = 5e-71
Identities = 138/178 (77%), Positives = 150/178 (83%), Gaps = 9/178 (5%)
Frame = +3
Query: 307 AEAMNRYGSDHPDTRFDLELKDVSDIFSGTSFKIFSDSLECGGVIKVLCVPSGATKDSYI 366
AEAMN YGSD PDTRFDLELKDVSDIFSG+SFK+FSDSLECGGVIKV+CVPSGA K S
Sbjct: 3 AEAMNIYGSDRPDTRFDLELKDVSDIFSGSSFKVFSDSLECGGVIKVICVPSGAKKYSNS 182
Query: 367 L--------DIYNEAEKYGAKGLTTLWITKNG-IGGFSSLVSSMDSATIEELLRRCSAGP 417
+ IYNEA K GAKGL L IT+NG I G S+LVSSMDSAT E+LLRRCSAGP
Sbjct: 183 VLKKSGANGGIYNEAHKSGAKGLPFLKITENGDIEGISALVSSMDSATREDLLRRCSAGP 362
Query: 418 SDLILFHAGHHASVNKTLGHLRVYVAHKLGLIDHAKHSILWITDFPMFKWDDSKQRLE 475
SDLILF GHHASVNKTL LRVYVAH+LGLIDHA+HSILWITDFPMF+W+DS+QRLE
Sbjct: 363 SDLILFAVGHHASVNKTLDRLRVYVAHELGLIDHARHSILWITDFPMFEWNDSEQRLE 536
>BI264098 similar to GP|13872951|dbj putative aspartate--tRNA ligase {Oryza
sativa (japonica cultivar-group)}, partial (9%)
Length = 492
Score = 244 bits (622), Expect = 7e-65
Identities = 129/171 (75%), Positives = 138/171 (80%), Gaps = 1/171 (0%)
Frame = +2
Query: 296 ELPNPFPRLTYAEAMNRYGSDHPDTRFDLELKDVSDIFSGTSFKIFSDSLECGGVIKVLC 355
ELPNPFPRLTYAEAMNRYGSD PDTRFDLELKDVSDIFS +SFK+FSDSLECGGVIKVLC
Sbjct: 8 ELPNPFPRLTYAEAMNRYGSDRPDTRFDLELKDVSDIFSRSSFKVFSDSLECGGVIKVLC 187
Query: 356 VPSGATKDSYILDIYNEAEKYGAKGLTTLWITKNG-IGGFSSLVSSMDSATIEELLRRCS 414
VPSGA K +GA GL L IT+NG I G S+LVSSMD AT +LLRRCS
Sbjct: 188 VPSGAKK----------YRNFGAFGLPFLKITENGDIKGISALVSSMDPATTVDLLRRCS 337
Query: 415 AGPSDLILFHAGHHASVNKTLGHLRVYVAHKLGLIDHAKHSILWITDFPMF 465
AGPSDLILF GHHASVNKTLG L V VA++L LIDHA+HSILW TDFPMF
Sbjct: 338 AGPSDLILFAVGHHASVNKTLGCLXVSVANELXLIDHARHSILWXTDFPMF 490
>CB065281 similar to SP|Q51422|SYD_ Aspartyl-tRNA synthetase (EC 6.1.1.12)
(Aspartate--tRNA ligase) (AspRS). {Pseudomonas
aeruginosa}, partial (32%)
Length = 599
Score = 103 bits (256), Expect = 2e-22
Identities = 63/168 (37%), Positives = 93/168 (54%)
Frame = -2
Query: 58 RTHHCGELSSNDVGKTVRLCGWAELHRSLGGVAFLILRDQTGIIQVKTRLDEFPVAHSAN 117
R+H+CG+L+ + G+ V LCGW R GGV FL +RD+ G+ QV D +A+
Sbjct: 565 RSHYCGQLNESLDGQEVTLCGWVHRRRDHGGVIFLDIRDREGMAQVVFDPDRAETFAAAD 386
Query: 118 KNLRREYVAAIQGVVRSRSTQSINYEMKTGFIEVAANEVQELNSRNAKLTLIPELNSDDA 177
+ +R EYV I G VR R ++N M +G IE+ ++ LN A+ P D
Sbjct: 385 R-VRSEYVVQITGKVRKRPDGAVNANMASGAIEILGYQLNVLN--EAETPPFPLNEYSDV 215
Query: 178 EGSPNEEIRLRYRCLDLRKQQMNSNILLRHNVVKLIRRYLEDIHGFVE 225
EE RLRYR +DLR+ +M + LR + IRRYL++ +GF++
Sbjct: 214 ----GEETRLRYRFIDLRRPEMADKLRLRSRITSSIRRYLDE-NGFLD 86
>TC87396 similar to GP|21537130|gb|AAM61471.1 unknown {Arabidopsis
thaliana}, partial (94%)
Length = 1429
Score = 30.0 bits (66), Expect = 2.1
Identities = 15/30 (50%), Positives = 19/30 (63%)
Frame = +2
Query: 12 ISIRRSPSLRKTKIPLPYYEKLKPKTKKAI 41
IS S LR + IPLP Y K+K KTK+ +
Sbjct: 59 ISFLFSTCLRYSSIPLPSYHKIK*KTKEEV 148
>TC80265 similar to GP|15450695|gb|AAK96619.1 AT5g13640/MSH12_10
{Arabidopsis thaliana}, partial (38%)
Length = 855
Score = 28.9 bits (63), Expect = 4.6
Identities = 11/33 (33%), Positives = 23/33 (69%)
Frame = -1
Query: 181 PNEEIRLRYRCLDLRKQQMNSNILLRHNVVKLI 213
P+E +++ +RCL L N+N++L H+ ++L+
Sbjct: 510 PSEHLKMVHRCLQLP*YIWNTNLVLLHHPLELV 412
>TC91124 weakly similar to GP|15809820|gb|AAL06838.1 At1g29690/F15D2_24
{Arabidopsis thaliana}, partial (37%)
Length = 653
Score = 28.1 bits (61), Expect = 7.9
Identities = 15/35 (42%), Positives = 22/35 (62%), Gaps = 1/35 (2%)
Frame = -1
Query: 13 SIRRSPSLRKTKIPLPYYEKL-KPKTKKAITFKKP 46
+IR + LRK+KIP+ + K+ KP + T KKP
Sbjct: 308 NIRNNKFLRKSKIPIMFLIKMNKPGPRCPFTVKKP 204
>TC79892 similar to GP|15010706|gb|AAK74012.1 At2g25350/F13B15.1
{Arabidopsis thaliana}, partial (92%)
Length = 1340
Score = 28.1 bits (61), Expect = 7.9
Identities = 10/29 (34%), Positives = 20/29 (68%)
Frame = -2
Query: 344 SLECGGVIKVLCVPSGATKDSYILDIYNE 372
S+ GGV+ +LC+ G+ + ++L ++NE
Sbjct: 196 SVGVGGVLGLLCLCGGSVRFCFVLTVFNE 110
>BF633115 weakly similar to GP|15809820|gb At1g29690/F15D2_24 {Arabidopsis
thaliana}, partial (48%)
Length = 630
Score = 28.1 bits (61), Expect = 7.9
Identities = 15/35 (42%), Positives = 22/35 (62%), Gaps = 1/35 (2%)
Frame = -3
Query: 13 SIRRSPSLRKTKIPLPYYEKL-KPKTKKAITFKKP 46
+IR + LRK+KIP+ + K+ KP + T KKP
Sbjct: 223 NIRNNKFLRKSKIPIMFLIKMNKPGPRCPFTVKKP 119
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.324 0.139 0.420
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 14,784,883
Number of Sequences: 36976
Number of extensions: 203846
Number of successful extensions: 1201
Number of sequences better than 10.0: 18
Number of HSP's better than 10.0 without gapping: 1181
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1194
length of query: 487
length of database: 9,014,727
effective HSP length: 100
effective length of query: 387
effective length of database: 5,317,127
effective search space: 2057728149
effective search space used: 2057728149
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)
S2: 60 (27.7 bits)
Medicago: description of AC146557.6