BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146554.7 - phase: 0 /pseudo
(1014 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
AJ497987 weakly similar to GP|9927273|dbj Similar to Arabidopsis... 309 4e-84
CB065285 weakly similar to PIR|A84500|A84 probable retroelement ... 280 2e-75
BG587127 weakly similar to PIR|H84506|H84 probable retroelement ... 66 8e-11
TC92306 47 5e-05
TC83629 weakly similar to GP|6692261|gb|AAF24611.1| putative RNa... 43 5e-04
AW684517 similar to GP|9927273|dbj Similar to Arabidopsis thalia... 38 0.002
TC83398 similar to PIR|T05150|T05150 hypothetical protein F18E5.... 35 0.11
BE240731 weakly similar to PIR|E84647|E846 hypothetical protein ... 33 0.42
BG586326 similar to PIR|G84493|G8 probable retroelement pol poly... 33 0.72
BG587101 similar to GP|6691191|gb F7F22.15 {Arabidopsis thaliana... 32 0.94
TC86742 similar to PIR|T01541|T01541 hypothetical protein A_IG00... 32 1.2
TC81017 weakly similar to GP|21632795|gb|AAL47185.1 phosphoinosi... 30 6.1
TC79625 similar to GP|6223643|gb|AAF05857.1| putative short-chai... 29 8.0
CA918150 similar to GP|1402894|emb| adenine phosphoribosyltransf... 29 8.0
AL375832 29 8.0
>AJ497987 weakly similar to GP|9927273|dbj Similar to Arabidopsis thaliana
chromosome II BAC F26H6; putative retroelement pol
polyprotein, partial (1%)
Length = 636
Score = 309 bits (791), Expect = 4e-84
Identities = 142/161 (88%), Positives = 155/161 (96%)
Frame = -2
Query: 638 AWAAKHLRHYMVNHTTWLMSKMDPIKYIFEKPALTGRIARWQMLLSEYDIVYRTQKAIKG 697
AWAAK LRHYM+NHTTWL+SKMDPIKYIFEKPALTGRIARWQMLLSEYDI YR+QKAIKG
Sbjct: 617 AWAAKRLRHYMINHTTWLVSKMDPIKYIFEKPALTGRIARWQMLLSEYDIEYRSQKAIKG 438
Query: 698 SILANHLAHQPIEDYQPIKFDFPDKEIMYLKMKDCDEPVFGEGPDPESE*GLIFDGAVNV 757
SILA+HLAHQP+EDY+PIKFDFPD+EIMYLKMKDCDEP+FGEGPDP+S GLIFDGAVNV
Sbjct: 437 SILADHLAHQPLEDYRPIKFDFPDEEIMYLKMKDCDEPLFGEGPDPDSVWGLIFDGAVNV 258
Query: 758 YGSGIGAVLITPKGTHIPFTARIQFDCTNNIVEYEACIMGI 798
YG+GIGAVL+TPKG HIPFTAR++FDCTNNI EYEACIMGI
Sbjct: 257 YGNGIGAVLLTPKGAHIPFTARLRFDCTNNIAEYEACIMGI 135
>CB065285 weakly similar to PIR|A84500|A84 probable retroelement gag/pol
polyprotein [imported] - Arabidopsis thaliana, partial
(2%)
Length = 592
Score = 280 bits (717), Expect = 2e-75
Identities = 136/196 (69%), Positives = 158/196 (80%), Gaps = 8/196 (4%)
Frame = -2
Query: 728 KMKDCDEPVFGEGPDPESE*GLIFDGAVNVYGSGIGAVLITPKGTHIPFTARIQFDCTNN 787
K KDC+EP+ GEGPDP S+ GL+FDGAVN YG GIGAV+++P+G +IPFTARI F+CTNN
Sbjct: 591 KSKDCEEPLIGEGPDPNSKWGLVFDGAVNAYGKGIGAVIVSPQGHYIPFTARILFECTNN 412
Query: 788 IVEYEACIMGIEEAIDLRIKKIVIYGDLALVINQIKGEWETRHPGLIPYRDYARRLLTFF 847
+ EYEACI GIEEAID+RIK + IYGD ALVINQIKGEWET H LIPYRDYARRLLT+F
Sbjct: 411 MAEYEACIFGIEEAIDMRIKHLDIYGDSALVINQIKGEWETHHANLIPYRDYARRLLTYF 232
Query: 848 NKVELHHVPRDENQMADALATLSSMINVNGHNTVLVINVQFLDRPAYVFA--------AE 899
KVELHH+PRDENQMADALATLSSM VN N V +I VQ L+RP++VFA E
Sbjct: 231 TKVELHHIPRDENQMADALATLSSMFRVNHWNDVPIIKVQRLERPSHVFAIGNVIDQTGE 52
Query: 900 AVDDDKPWYHDIQVFV 915
V D KPWY+DI+ F+
Sbjct: 51 NVVDYKPWYYDIKQFL 4
>BG587127 weakly similar to PIR|H84506|H84 probable retroelement pol
polyprotein [imported] - Arabidopsis thaliana, partial
(13%)
Length = 415
Score = 65.9 bits (159), Expect = 8e-11
Identities = 39/109 (35%), Positives = 56/109 (50%)
Frame = +3
Query: 763 GAVLITPKGTHIPFTARIQFDCTNNIVEYEACIMGIEEAIDLRIKKIVIYGDLALVINQI 822
G L +P + + R++F +NN YEA I G+ A L+I+ I Y D LV +Q
Sbjct: 3 GIRLTSPTNEILKQSFRLEFHASNNETNYEALIAGVRLAHGLKIRNIHAYCDSQLVASQF 182
Query: 823 KGEWETRHPGLIPYRDYARRLLTFFNKVELHHVPRDENQMADALATLSS 871
GE+E R + Y ++L + L +PR EN ADALA L+S
Sbjct: 183 SGEYEARDELMDTYLKLVQKLAQKLDYFALTRIPRSENVQADALAALAS 329
>TC92306
Length = 521
Score = 46.6 bits (109), Expect = 5e-05
Identities = 25/73 (34%), Positives = 36/73 (49%)
Frame = +2
Query: 795 IMGIEEAIDLRIKKIVIYGDLALVINQIKGEWETRHPGLIPYRDYARRLLTFFNKVELHH 854
I+G+ EA + + + + GD V Q G W+ +P L + A L + F V + H
Sbjct: 2 ILGLNEARNQGYEHVHVRGDFQXVCKQFXGSWKVNNPNLRNLCNXAVELKSNFKSVSVEH 181
Query: 855 VPRDENQMADALA 867
VPR N ADA A
Sbjct: 182 VPRGXNXAADAQA 220
>TC83629 weakly similar to GP|6692261|gb|AAF24611.1| putative RNase H
{Arabidopsis thaliana}, partial (21%)
Length = 1071
Score = 43.1 bits (100), Expect = 5e-04
Identities = 29/106 (27%), Positives = 47/106 (43%)
Frame = +2
Query: 755 VNVYGSGIGAVLITPKGTHIPFTARIQFDCTNNIVEYEACIMGIEEAIDLRIKKIVIYGD 814
+N +G GA+L+ G+ + + T EY A ++G++ A K + GD
Sbjct: 584 INRGPAGAGALLLAEDGSLLYGFRQGLGHQTKESAEYRALLLGLKHASMKGFKYVTAKGD 763
Query: 815 LALVINQIKGEWETRHPGLIPYRDYARRLLTFFNKVELHHVPRDEN 860
LVINQI W+ + L A L F+ + H+ R+ N
Sbjct: 764 SELVINQILDPWKIKDEHLKKLCAEALELSDNFHSFRIQHISRERN 901
>AW684517 similar to GP|9927273|dbj Similar to Arabidopsis thaliana
chromosome II BAC F26H6; putative retroelement pol
polyprotein, partial (1%)
Length = 488
Score = 37.7 bits (86), Expect(2) = 0.002
Identities = 39/118 (33%), Positives = 53/118 (44%)
Frame = +2
Query: 81 FKSWRYKLLSVAYLADHGFMTLGQ*HPLSTRS*SL*VMES*LL*VVSQPF*SAICLLSLL 140
F+SW L D G+MTL +* TRS*+L* M+ L
Sbjct: 8 FRSWISMLRIAVSWEDLGYMTLAR*PLPYTRS*NL*RMD--------------------L 127
Query: 141 LVVIVQTGRHSKGSLPKKV*VRLRLVWVR*KTPRG*FRKAKLKVGVS**SYQRTSAKK 198
L ++ G K P+ RL L W+ *+TPR FR+ +L G S* + R S +K
Sbjct: 128 LRELLFKGCLWKVQSPR----RLELQWLL*RTPRRLFRRDRLPTGAS*SNSVRISERK 289
Score = 22.3 bits (46), Expect(2) = 0.002
Identities = 8/14 (57%), Positives = 11/14 (78%)
Frame = +1
Query: 216 GSFHSAGFIHDSLE 229
G+FHSAGF++ E
Sbjct: 325 GTFHSAGFVNTLAE 366
>TC83398 similar to PIR|T05150|T05150 hypothetical protein F18E5.40 -
Arabidopsis thaliana, partial (6%)
Length = 766
Score = 35.4 bits (80), Expect = 0.11
Identities = 29/96 (30%), Positives = 47/96 (48%), Gaps = 4/96 (4%)
Frame = +2
Query: 783 DCTNNIV--EYEACIMGIEEAIDLRIKKIVIYGDLALVINQIKGEWETRHPGLIPYRDYA 840
D +N+I+ E A +MG++ A L I +V Y D +N I G P ++ Y YA
Sbjct: 122 DHSNDILFAELHAILMGLQLAQTLNIVDVVCYSDSLHYVNLING------PSVV-YHAYA 280
Query: 841 RRLLTFFNKVELH--HVPRDENQMADALATLSSMIN 874
+ + + L H R+ N+ AD LA L + ++
Sbjct: 281 TLIQDIKDLIRLSKLHTLREGNRCADFLAKLGASVD 388
>BE240731 weakly similar to PIR|E84647|E846 hypothetical protein At2g25360
[imported] - Arabidopsis thaliana, partial (18%)
Length = 603
Score = 33.5 bits (75), Expect = 0.42
Identities = 13/43 (30%), Positives = 23/43 (53%)
Frame = +1
Query: 789 VEYEACIMGIEEAIDLRIKKIVIYGDLALVINQIKGEWETRHP 831
VE A I G I L +K+++ +GD + + G+W ++ P
Sbjct: 94 VELMALIEGFNAVIALDLKRVIYFGDYYTLFQHLSGKWASKQP 222
>BG586326 similar to PIR|G84493|G8 probable retroelement pol polyprotein
[imported] - Arabidopsis thaliana, partial (13%)
Length = 736
Score = 32.7 bits (73), Expect = 0.72
Identities = 19/72 (26%), Positives = 37/72 (51%)
Frame = +2
Query: 639 WAAKHLRHYMVNHTTWLMSKMDPIKYIFEKPALTGRIARWQMLLSEYDIVYRTQKAIKGS 698
+A K R Y+ + + +KYIF +P L R RW +++YD+ T K +
Sbjct: 191 FALKIWRSYLYGAKVQIHTDHKSLKYIFTQPELNLRQRRWMEFVADYDLDI-TYYPGKAN 367
Query: 699 ILANHLAHQPIE 710
++A+ L+ + ++
Sbjct: 368 LVADALSRRRVD 403
>BG587101 similar to GP|6691191|gb F7F22.15 {Arabidopsis thaliana}, partial
(10%)
Length = 624
Score = 32.3 bits (72), Expect = 0.94
Identities = 16/63 (25%), Positives = 34/63 (53%), Gaps = 1/63 (1%)
Frame = +2
Query: 938 RFFLNEDVLYKRNFDGVLLRCVNKHEAGKLMHEIHEGSFGTHSCGHAKL*RSL-RGEESF 996
R+ +E LYK+ D + +RCV + E ++ H G++ GH + +++ + +++
Sbjct: 17 RYLWDEPFLYKQCADNIYIRCVAEEEIPGILFHCH----GSNYAGHFAVSKTVSKIQQAG 184
Query: 997 LWW 999
WW
Sbjct: 185 FWW 193
>TC86742 similar to PIR|T01541|T01541 hypothetical protein A_IG005I10.16 -
Arabidopsis thaliana, partial (19%)
Length = 2073
Score = 32.0 bits (71), Expect = 1.2
Identities = 26/85 (30%), Positives = 44/85 (51%), Gaps = 7/85 (8%)
Frame = -1
Query: 790 EYEACIMGIEEAIDLRIKKIVIYGDLALVIN---QIKGEWETRHPGLIPYRDYAR--RLL 844
E+ AC++ IE+A++L + I + D V+N +I G IP++ R +
Sbjct: 393 EFCACMIAIEKAMELGLNNICLETDSLKVVNAFHKIVG---------IPWQMRVRWHNCI 241
Query: 845 TFFNKVE--LHHVPRDENQMADALA 867
F + + H+PR+ N +ADALA
Sbjct: 240 RFCHSIACVCVHIPREGNLVADALA 166
>TC81017 weakly similar to GP|21632795|gb|AAL47185.1
phosphoinositide-dependent protein kinase-1 beta {Mus
musculus}, partial (32%)
Length = 721
Score = 29.6 bits (65), Expect = 6.1
Identities = 12/22 (54%), Positives = 17/22 (76%)
Frame = -1
Query: 939 FFLNEDVLYKRNFDGVLLRCVN 960
FFL +DVL+ +NF GV L C++
Sbjct: 124 FFLLDDVLFLQNFYGVFLECLS 59
>TC79625 similar to GP|6223643|gb|AAF05857.1| putative short-chain type
dehydrogenase/reductase {Arabidopsis thaliana}, partial
(85%)
Length = 1039
Score = 29.3 bits (64), Expect = 8.0
Identities = 11/22 (50%), Positives = 13/22 (59%)
Frame = +1
Query: 400 HHTLGCFLLCGDAIWVDKCRCH 421
HHTLG FL+C + V CH
Sbjct: 886 HHTLGYFLICSQIVIVMMSTCH 951
>CA918150 similar to GP|1402894|emb| adenine phosphoribosyltransferase
{Arabidopsis thaliana}, partial (11%)
Length = 514
Score = 29.3 bits (64), Expect = 8.0
Identities = 18/45 (40%), Positives = 26/45 (57%)
Frame = -2
Query: 918 QKYPPGASNKDKKTLRKLSSRFFLNEDVLYKRNFDGVLLRCVNKH 962
Q + GA + DK++ KLSS +L+ D++Y R V L NKH
Sbjct: 231 QTFTCGALSPDKRSSWKLSS-LYLDLDLVYVRISSNVWLTISNKH 100
>AL375832
Length = 535
Score = 29.3 bits (64), Expect = 8.0
Identities = 31/96 (32%), Positives = 44/96 (45%), Gaps = 7/96 (7%)
Frame = +3
Query: 247 IMNENRRFCHVIYYLIFIFWCFFWKNGNIKIQKNRSLFLLKFSK-------SKVCIYSLK 299
+MN+N RF H ++ F FF + + + R FL+ F K LK
Sbjct: 237 LMNKNCRFFHDCFF----FSAFFLEKW*CQKKPKRLYFLINFKNLHEKKKIQKTKKLFLK 404
Query: 300 SIMNHV*TEHK*AY*TQ*PHASSQL*GPGLRGYSRE 335
S +++ E * *TQ* +ASS+L* P RE
Sbjct: 405 SSNHYMQVEP**TR*TQ*FYASSKL*FPVYEPKMRE 512
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.352 0.157 0.561
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 33,852,205
Number of Sequences: 36976
Number of extensions: 513668
Number of successful extensions: 4902
Number of sequences better than 10.0: 30
Number of HSP's better than 10.0 without gapping: 2070
Number of HSP's successfully gapped in prelim test: 239
Number of HSP's that attempted gapping in prelim test: 2746
Number of HSP's gapped (non-prelim): 2475
length of query: 1014
length of database: 9,014,727
effective HSP length: 106
effective length of query: 908
effective length of database: 5,095,271
effective search space: 4626506068
effective search space used: 4626506068
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 14 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 38 (22.0 bits)
S2: 63 (28.9 bits)
Medicago: description of AC146554.7