BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146554.5 + phase: 0
(236 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC82590 similar to PIR|T50806|T50806 zinc finger-like protein - ... 69 2e-12
BG449113 similar to PIR|S55881|S558 CCHH finger protein 1 - Arab... 62 3e-10
BI308410 similar to PIR|S55882|S558 CCHH finger protein 2 - Arab... 62 3e-10
TC89540 similar to PIR|B96686|B96686 probable C2H2-type zinc fin... 61 4e-10
TC88476 homologue to GP|21592423|gb|AAM64374.1 zinc finger prote... 60 1e-09
TC82737 homologue to PIR|B96686|B96686 probable C2H2-type zinc f... 59 2e-09
AW686309 homologue to PIR|S55886|S558 CCHH finger protein 6 - Ar... 57 6e-09
TC80421 similar to PIR|D96707|D96707 probable zinc finger protei... 53 1e-07
TC88893 similar to GP|6478938|gb|AAF14043.1| putative C2H2-type ... 47 6e-06
AL380167 homologue to PIR|S55887|S558 CCHH finger protein 7 - Ar... 45 2e-05
TC81612 similar to GP|2346978|dbj|BAA21923.1 ZPT2-14 {Petunia x ... 45 3e-05
TC88075 homologue to GP|2346978|dbj|BAA21923.1 ZPT2-14 {Petunia ... 44 5e-05
BG648193 similar to GP|2346976|dbj| ZPT2-13 {Petunia x hybrida},... 42 2e-04
TC88653 similar to GP|1786134|dbj|BAA19110.1 PEThy;ZPT2-5 {Petun... 42 3e-04
TC81921 homologue to GP|2346978|dbj|BAA21923.1 ZPT2-14 {Petunia ... 39 0.002
TC82573 similar to PIR|T09602|T09602 probable zinc finger protei... 38 0.004
TC86494 homologue to GP|7228329|emb|CAB77055.1 putative TFIIIA (... 37 0.005
TC77176 homologue to GP|7228329|emb|CAB77055.1 putative TFIIIA (... 36 0.011
TC82775 similar to PIR|T52382|T52382 zinc finger protein ZPT4-4 ... 35 0.025
TC93387 similar to GP|1786136|dbj|BAA19111.1 PEThy;ZPT2-6 {Petun... 35 0.025
>TC82590 similar to PIR|T50806|T50806 zinc finger-like protein - Arabidopsis
thaliana, partial (19%)
Length = 690
Score = 68.9 bits (167), Expect = 2e-12
Identities = 49/125 (39%), Positives = 64/125 (51%), Gaps = 9/125 (7%)
Frame = +2
Query: 28 LENGGHSSSFQNTLDEPLPEQKVVEFSCNYCDKKFSTSQALGGHQNAHKRERVLKKMEDR 87
L+ G S NTLD EQ+V FSCNYC +KF +SQALGGHQNAHKRER + K R
Sbjct: 275 LDVGNSLSQNNNTLDV---EQRV--FSCNYCHRKFYSSQALGGHQNAHKRERSIAKRGQR 439
Query: 88 RREEEMDLTLRFSSFIHYQGHSYFRSANL-------HNPIGVHVTNAF--PSWIGSPYGG 138
+ + F I + SY A+L + P+G+ + PS + S G
Sbjct: 440 FGTQIIASASAFGFPILHNKQSYANMASLPLYGAFGNKPLGIQAHSMIHKPSHVSS--NG 613
Query: 139 YGGVY 143
+G Y
Sbjct: 614 FGNSY 628
>BG449113 similar to PIR|S55881|S558 CCHH finger protein 1 - Arabidopsis
thaliana, partial (15%)
Length = 632
Score = 61.6 bits (148), Expect = 3e-10
Identities = 33/75 (44%), Positives = 46/75 (61%)
Frame = +1
Query: 53 FSCNYCDKKFSTSQALGGHQNAHKRERVLKKMEDRRREEEMDLTLRFSSFIHYQGHSYFR 112
F+CNYC +KF +SQALGGHQNAHKRER + K R M +SF+H H + R
Sbjct: 229 FTCNYCKRKFFSSQALGGHQNAHKRERSIAK----RGRRTMFSATGTTSFLH--NHLHHR 390
Query: 113 SANLHNPIGVHVTNA 127
AN+ + + ++ N+
Sbjct: 391 YANMASLLPLYGANS 435
>BI308410 similar to PIR|S55882|S558 CCHH finger protein 2 - Arabidopsis
thaliana, partial (46%)
Length = 492
Score = 61.6 bits (148), Expect = 3e-10
Identities = 39/96 (40%), Positives = 52/96 (53%)
Frame = +3
Query: 28 LENGGHSSSFQNTLDEPLPEQKVVEFSCNYCDKKFSTSQALGGHQNAHKRERVLKKMEDR 87
LE SSS +++++ + FSCNYC +KF +SQALGGHQNAHK ER L K
Sbjct: 48 LEPSSSSSSSSSSMEQRI-------FSCNYCQRKFYSSQALGGHQNAHKLERTLAK---- 194
Query: 88 RREEEMDLTLRFSSFIHYQGHSYFRSANLHNPIGVH 123
+ EM ++ SS+ H S N H +G H
Sbjct: 195 -KSREMSSAMQ-SSYAELPEHPSNFSTNYH--LGSH 290
>TC89540 similar to PIR|B96686|B96686 probable C2H2-type zinc finger protein
F15E12.19 [imported] - Arabidopsis thaliana, partial
(28%)
Length = 1639
Score = 60.8 bits (146), Expect = 4e-10
Identities = 45/122 (36%), Positives = 58/122 (46%), Gaps = 2/122 (1%)
Frame = +1
Query: 11 DFLTLRLGTHTKSTSKPLENGGHSSSFQNTLD-EPLPEQKVVEFSCNYCDKKFSTSQALG 69
D +TL L + S+ L +SS + P FSCNYC +KF +SQALG
Sbjct: 448 DPMTLDLTLNFSSSEAELMGSSDASSEVGAAEVHASPSVTPRIFSCNYCKRKFYSSQALG 627
Query: 70 GHQNAHKRERVLKKMEDRRREEEMDLTLRFSSFIHYQGH-SYFRSANLHNPIGVHVTNAF 128
GHQNAHKRER + K R T R++S H S FRS + +H +
Sbjct: 628 GHQNAHKRERTMAKRAMRMGM----FTERYTSLASLPLHGSPFRSLGVEAHSAMHQRHMQ 795
Query: 129 PS 130
PS
Sbjct: 796 PS 801
>TC88476 homologue to GP|21592423|gb|AAM64374.1 zinc finger protein 5 ZFP5
{Arabidopsis thaliana}, partial (21%)
Length = 945
Score = 59.7 bits (143), Expect = 1e-09
Identities = 36/101 (35%), Positives = 57/101 (55%), Gaps = 1/101 (0%)
Frame = +2
Query: 18 GTHTKSTSKPLENGGHSSSFQNTLDEPLPEQKVVEFSCNYCDKKFSTSQALGGHQNAHKR 77
G + ++S + +GG + + E+K F C YC K+F+ SQALGGHQNAHK+
Sbjct: 152 GDESVNSSNSISSGGDKIVQEKNSSKDQDERK---FECQYCFKEFANSQALGGHQNAHKK 322
Query: 78 ERVLKK-MEDRRREEEMDLTLRFSSFIHYQGHSYFRSANLH 117
ER+ KK ++ + R+ ++ L+ H+ G SY S + H
Sbjct: 323 ERLKKKRLQLQARKASINYYLQPFQKNHH-GFSYHGSDHTH 442
>TC82737 homologue to PIR|B96686|B96686 probable C2H2-type zinc finger
protein F15E12.19 [imported] - Arabidopsis thaliana,
partial (18%)
Length = 1006
Score = 58.9 bits (141), Expect = 2e-09
Identities = 31/55 (56%), Positives = 37/55 (66%)
Frame = +2
Query: 29 ENGGHSSSFQNTLDEPLPEQKVVEFSCNYCDKKFSTSQALGGHQNAHKRERVLKK 83
++ G S S + L Q+V FSCNYC +KF +SQALGGHQNAHKRER L K
Sbjct: 347 DSTGISFSSTSESSNELTIQRV--FSCNYCRRKFYSSQALGGHQNAHKRERTLAK 505
>AW686309 homologue to PIR|S55886|S558 CCHH finger protein 6 - Arabidopsis
thaliana, partial (25%)
Length = 669
Score = 57.0 bits (136), Expect = 6e-09
Identities = 41/122 (33%), Positives = 60/122 (48%), Gaps = 7/122 (5%)
Frame = +2
Query: 33 HSSSFQNTLDEPLPEQKVVEFSCNYCDKKFSTSQALGGHQNAHKRERVLKKMEDRRREEE 92
+ S Q+ EPL +K+ E C YC ++F+ SQALGGHQNAHK+ER L K +
Sbjct: 173 NQSPTQSESSEPLNGRKLYE--CQYCCREFANSQALGGHQNAHKKERQLLKRAQMQAAR- 343
Query: 93 MDLTLRFSSFIHYQGHSYFRSANLHNPIGVH-VTNAFPS------WIGSPYGGYGGVYMP 145
+F+ H+ F S+ P H + NA PS W+ + + GV++
Sbjct: 344 --------AFVPSHFHNRFISSPQWLPQQNHFLANAPPSQTRSSAWLCTTHAAGSGVFLA 499
Query: 146 NT 147
T
Sbjct: 500 PT 505
>TC80421 similar to PIR|D96707|D96707 probable zinc finger protein T22E19.1
[imported] - Arabidopsis thaliana, partial (37%)
Length = 1217
Score = 52.8 bits (125), Expect = 1e-07
Identities = 45/140 (32%), Positives = 62/140 (44%), Gaps = 6/140 (4%)
Frame = +2
Query: 2 PPTSESHVEDFLTLRLGTHTKSTSKPLEN----GGHSSSFQNTLDEPLPEQKVVEFSCNY 57
PP + +ED GT S S E+ GG SS + D ++ C Y
Sbjct: 125 PPPRQPPLED------GTGNTSPSGSTESVSGDGGASSINPTSGDR--------KYECQY 262
Query: 58 CDKKFSTSQALGGHQNAHKRERVLKKMEDRRREEEMDLTLRFSSFIHYQGHSYFRSAN-- 115
C ++F+ SQALGGHQNAHK+ER K + + SF+ S F +
Sbjct: 263 CCREFANSQALGGHQNAHKKERQQLKRAQLQASRNAAV-----SFVRNPIISAFAPPHHL 427
Query: 116 LHNPIGVHVTNAFPSWIGSP 135
L +P V + A PSW+ P
Sbjct: 428 LSSPGQVMMPTAPPSWVYMP 487
>TC88893 similar to GP|6478938|gb|AAF14043.1| putative C2H2-type zinc finger
protein {Arabidopsis thaliana}, partial (25%)
Length = 819
Score = 47.0 bits (110), Expect = 6e-06
Identities = 23/65 (35%), Positives = 38/65 (58%)
Frame = +1
Query: 29 ENGGHSSSFQNTLDEPLPEQKVVEFSCNYCDKKFSTSQALGGHQNAHKRERVLKKMEDRR 88
+N S + + D+P +V +SC +C + FS +QALGGH N H+R+R K++ +
Sbjct: 67 QNPNKSDQIRWSSDDP---GQVRSYSCTFCKRGFSNAQALGGHMNIHRRDRA--KLKQQS 231
Query: 89 REEEM 93
EE +
Sbjct: 232 SEENL 246
>AL380167 homologue to PIR|S55887|S558 CCHH finger protein 7 - Arabidopsis
thaliana, partial (11%)
Length = 476
Score = 45.4 bits (106), Expect = 2e-05
Identities = 18/23 (78%), Positives = 21/23 (91%)
Frame = +2
Query: 53 FSCNYCDKKFSTSQALGGHQNAH 75
FSC+YC +KF +SQALGGHQNAH
Sbjct: 407 FSCHYCRRKFFSSQALGGHQNAH 475
>TC81612 similar to GP|2346978|dbj|BAA21923.1 ZPT2-14 {Petunia x hybrida},
partial (50%)
Length = 612
Score = 44.7 bits (104), Expect = 3e-05
Identities = 21/53 (39%), Positives = 34/53 (63%), Gaps = 3/53 (5%)
Frame = +3
Query: 46 PEQKVVE---FSCNYCDKKFSTSQALGGHQNAHKRERVLKKMEDRRREEEMDL 95
P+QK E + C C+KKFS+ QALGGH+ +HKR ++ + E + + + + L
Sbjct: 162 PQQKSYENGEYECKTCNKKFSSFQALGGHRASHKRMKLAEGEELKEQAKSLSL 320
Score = 30.4 bits (67), Expect = 0.62
Identities = 20/57 (35%), Positives = 24/57 (42%), Gaps = 11/57 (19%)
Frame = +3
Query: 31 GGHSSSFQNTL---DEPLPEQ--------KVVEFSCNYCDKKFSTSQALGGHQNAHK 76
GGH +S + E L EQ K C+ C FS QALGGH H+
Sbjct: 240 GGHRASHKRMKLAEGEELKEQAKSLSLWNKPKMHECSICGMGFSLGQALGGHMRKHR 410
>TC88075 homologue to GP|2346978|dbj|BAA21923.1 ZPT2-14 {Petunia x hybrida},
partial (38%)
Length = 674
Score = 43.9 bits (102), Expect = 5e-05
Identities = 18/35 (51%), Positives = 26/35 (73%)
Frame = +3
Query: 51 VEFSCNYCDKKFSTSQALGGHQNAHKRERVLKKME 85
VEF C C++KFS+ QALGGH+ +HK+ ++L E
Sbjct: 138 VEFECKTCNRKFSSFQALGGHRASHKKLKLLDSEE 242
Score = 30.0 bits (66), Expect = 0.81
Identities = 10/22 (45%), Positives = 14/22 (63%)
Frame = +3
Query: 55 CNYCDKKFSTSQALGGHQNAHK 76
C+ C ++F QALGGH H+
Sbjct: 288 CSICGQEFKLGQALGGHMRRHR 353
>BG648193 similar to GP|2346976|dbj| ZPT2-13 {Petunia x hybrida}, partial
(43%)
Length = 629
Score = 42.4 bits (98), Expect = 2e-04
Identities = 19/38 (50%), Positives = 26/38 (68%), Gaps = 3/38 (7%)
Frame = +2
Query: 46 PEQKV---VEFSCNYCDKKFSTSQALGGHQNAHKRERV 80
P QK VE+ C C+KKF + QALGGH+ +HKR ++
Sbjct: 191 PNQKSFAPVEYECKTCNKKFPSFQALGGHRASHKRSKL 304
Score = 32.3 bits (72), Expect = 0.16
Identities = 12/22 (54%), Positives = 14/22 (63%)
Frame = +2
Query: 55 CNYCDKKFSTSQALGGHQNAHK 76
C+ C + FS QALGGH HK
Sbjct: 371 CSICGQNFSLGQALGGHMRRHK 436
>TC88653 similar to GP|1786134|dbj|BAA19110.1 PEThy;ZPT2-5 {Petunia x
hybrida}, partial (59%)
Length = 866
Score = 41.6 bits (96), Expect = 3e-04
Identities = 20/63 (31%), Positives = 34/63 (53%)
Frame = +1
Query: 24 TSKPLENGGHSSSFQNTLDEPLPEQKVVEFSCNYCDKKFSTSQALGGHQNAHKRERVLKK 83
+ K + +SS+F N + F C C ++FS+ QALGGH+ +HK+ R+++
Sbjct: 97 SDKIFDQVNYSSNFNNRV-----------FECKTCKRQFSSFQALGGHRASHKKPRLMEM 243
Query: 84 MED 86
D
Sbjct: 244 TSD 252
Score = 32.3 bits (72), Expect = 0.16
Identities = 22/68 (32%), Positives = 29/68 (42%), Gaps = 7/68 (10%)
Frame = +1
Query: 17 LGTHTKSTSKP----LENGG---HSSSFQNTLDEPLPEQKVVEFSCNYCDKKFSTSQALG 69
LG H S KP + + G H S +T K +C+ C +F QALG
Sbjct: 196 LGGHRASHKKPRLMEMTSDGDDHHGSILTSTT-------KAKTHACSICGLEFGIGQALG 354
Query: 70 GHQNAHKR 77
GH H+R
Sbjct: 355 GHMRRHRR 378
>TC81921 homologue to GP|2346978|dbj|BAA21923.1 ZPT2-14 {Petunia x hybrida},
partial (41%)
Length = 839
Score = 38.9 bits (89), Expect = 0.002
Identities = 17/48 (35%), Positives = 28/48 (57%)
Frame = +3
Query: 34 SSSFQNTLDEPLPEQKVVEFSCNYCDKKFSTSQALGGHQNAHKRERVL 81
S F+ T +V F C C+++F + QALGGH+ +HK+ R++
Sbjct: 192 SDQFEATYSSTTSNNRV--FECKTCNRQFPSFQALGGHRASHKKPRLM 329
Score = 33.1 bits (74), Expect = 0.095
Identities = 21/60 (35%), Positives = 27/60 (45%)
Frame = +3
Query: 17 LGTHTKSTSKPLENGGHSSSFQNTLDEPLPEQKVVEFSCNYCDKKFSTSQALGGHQNAHK 76
LG H S KP G + L P+ K E C+ C +F+ QALGGH H+
Sbjct: 288 LGGHRASHKKPRLMG---ENIDGQLLHTPPKPKTHE--CSICGLEFAIGQALGGHMRRHR 452
>TC82573 similar to PIR|T09602|T09602 probable zinc finger protein SCOF-1
cold-inducible - soybean, partial (65%)
Length = 857
Score = 37.7 bits (86), Expect = 0.004
Identities = 13/30 (43%), Positives = 23/30 (76%)
Frame = +3
Query: 51 VEFSCNYCDKKFSTSQALGGHQNAHKRERV 80
+ + C+ C+K+FS+ QALGGH+ +H++ V
Sbjct: 273 LSYKCSVCNKEFSSYQALGGHKASHRKNSV 362
Score = 34.3 bits (77), Expect = 0.043
Identities = 22/64 (34%), Positives = 26/64 (40%), Gaps = 5/64 (7%)
Frame = +3
Query: 17 LGTHTKSTSKPLENGG-----HSSSFQNTLDEPLPEQKVVEFSCNYCDKKFSTSQALGGH 71
LG H S K GG +SS T V C+ C + F T QALGGH
Sbjct: 324 LGGHKASHRKNSVGGGGDDHPSTSSAATTSSANTNGGGVRSHECSICHRSFPTGQALGGH 503
Query: 72 QNAH 75
+ H
Sbjct: 504 KRCH 515
>TC86494 homologue to GP|7228329|emb|CAB77055.1 putative TFIIIA (or
kruppel)-like zinc finger protein {Medicago sativa},
partial (39%)
Length = 1092
Score = 37.4 bits (85), Expect = 0.005
Identities = 20/59 (33%), Positives = 31/59 (51%)
Frame = +2
Query: 17 LGTHTKSTSKPLENGGHSSSFQNTLDEPLPEQKVVEFSCNYCDKKFSTSQALGGHQNAH 75
LG H K++ + + S++ T+ + K+ E C+ C K F T QALGGH+ H
Sbjct: 353 LGGH-KASHRKSSSENQSTTVNETISVSVSTSKMHE--CSICHKSFPTGQALGGHKRCH 520
Score = 33.5 bits (75), Expect = 0.073
Identities = 19/76 (25%), Positives = 34/76 (44%)
Frame = +2
Query: 2 PPTSESHVEDFLTLRLGTHTKSTSKPLENGGHSSSFQNTLDEPLPEQKVVEFSCNYCDKK 61
PPT E ++ L + ++ + S PL+ + C+ C+K
Sbjct: 218 PPTEEEYLALCLIMLSQSNNQIQSSPLK---------------------LNHKCSVCNKA 334
Query: 62 FSTSQALGGHQNAHKR 77
F + QALGGH+ +H++
Sbjct: 335 FPSYQALGGHKASHRK 382
>TC77176 homologue to GP|7228329|emb|CAB77055.1 putative TFIIIA (or
kruppel)-like zinc finger protein {Medicago sativa},
partial (96%)
Length = 1005
Score = 36.2 bits (82), Expect = 0.011
Identities = 16/49 (32%), Positives = 30/49 (60%), Gaps = 1/49 (2%)
Frame = +2
Query: 30 NGGHSSSFQNTLDEPLPEQKV-VEFSCNYCDKKFSTSQALGGHQNAHKR 77
+G ++ + ++ P P V + C+ C+K FS+ QALGGH+ +H++
Sbjct: 293 SGNNNDNKTESVPVPAPLTTVKLSHKCSVCNKAFSSYQALGGHKASHRK 439
Score = 33.1 bits (74), Expect = 0.095
Identities = 12/21 (57%), Positives = 14/21 (66%)
Frame = +2
Query: 55 CNYCDKKFSTSQALGGHQNAH 75
C+ C K F T QALGGH+ H
Sbjct: 539 CSICHKSFPTGQALGGHKRCH 601
>TC82775 similar to PIR|T52382|T52382 zinc finger protein ZPT4-4 C2H2-type
[imported] - garden petunia, partial (17%)
Length = 1152
Score = 35.0 bits (79), Expect = 0.025
Identities = 14/48 (29%), Positives = 26/48 (54%), Gaps = 2/48 (4%)
Frame = +3
Query: 45 LPEQKVVEF--SCNYCDKKFSTSQALGGHQNAHKRERVLKKMEDRRRE 90
+ E ++ EF +C +C K F ++LGGH +H +K E + ++
Sbjct: 120 MEEDQIQEFKHACKFCSKSFPCGRSLGGHMRSHINNLSAEKAEHKEKQ 263
Score = 31.2 bits (69), Expect = 0.36
Identities = 15/34 (44%), Positives = 20/34 (58%), Gaps = 1/34 (2%)
Frame = +3
Query: 55 CNYCDKKFSTSQALGGHQNAH-KRERVLKKMEDR 87
C C K F + QAL GH H ++ERV +ED+
Sbjct: 408 CKECGKCFHSWQALFGHMKCHSEKERVSNSLEDQ 509
>TC93387 similar to GP|1786136|dbj|BAA19111.1 PEThy;ZPT2-6 {Petunia x
hybrida}, partial (17%)
Length = 650
Score = 35.0 bits (79), Expect = 0.025
Identities = 11/29 (37%), Positives = 21/29 (71%)
Frame = +1
Query: 53 FSCNYCDKKFSTSQALGGHQNAHKRERVL 81
+ C C++ F + QALGGH+ +HK+ +++
Sbjct: 472 YECKTCNRTFPSFQALGGHRASHKKPKIM 558
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.315 0.132 0.398
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 8,362,008
Number of Sequences: 36976
Number of extensions: 129688
Number of successful extensions: 720
Number of sequences better than 10.0: 62
Number of HSP's better than 10.0 without gapping: 705
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 720
length of query: 236
length of database: 9,014,727
effective HSP length: 93
effective length of query: 143
effective length of database: 5,575,959
effective search space: 797362137
effective search space used: 797362137
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.5 bits)
S2: 57 (26.6 bits)
Medicago: description of AC146554.5