BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146554.3 - phase: 0
(247 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC79059 homologue to GP|22138108|gb|AAM93434.1 40S ribosomal S4 ... 498 e-142
TC87437 homologue to GP|22138108|gb|AAM93434.1 40S ribosomal S4 ... 472 e-134
TC76614 homologue to GP|22138108|gb|AAM93434.1 40S ribosomal S4 ... 468 e-132
TC76613 homologue to GP|22138108|gb|AAM93434.1 40S ribosomal S4 ... 468 e-132
BQ146628 homologue to GP|22138108|gb 40S ribosomal S4 protein {G... 236 6e-63
BQ149101 similar to GP|22138108|gb| 40S ribosomal S4 protein {Gl... 91 4e-31
AL381307 similar to SP|O59950|RS4_Y 40S ribosomal protein S4 (S7... 120 6e-28
TC76625 similar to SP|O81363|RS4_PRUAR 40S ribosomal protein S4.... 66 1e-11
AW329812 homologue to GP|7340791|emb| ribosomal protein L23 {Spi... 55 2e-08
AW689115 weakly similar to GP|20873469|em putative metallophosph... 30 1.1
BQ144197 homologue to GP|1841930|emb| ROX protein {Mus musculus}... 28 2.5
TC79131 similar to GP|14031063|gb|AAK52092.1 WD-40 repeat protei... 28 3.3
TC90027 similar to GP|4206789|gb|AAD11809.1| syntaxin-related pr... 28 3.3
TC87302 similar to GP|19911199|dbj|BAB86926. glucosyltransferase... 27 5.6
>TC79059 homologue to GP|22138108|gb|AAM93434.1 40S ribosomal S4 protein
{Glycine max}, complete
Length = 979
Score = 498 bits (1282), Expect = e-142
Identities = 247/247 (100%), Positives = 247/247 (100%)
Frame = +1
Query: 1 MLDKLGGAFAPKPSSGPHKSRECLPLILILRNRLKYALTYREVIAILMQRHVLVDGKVRT 60
MLDKLGGAFAPKPSSGPHKSRECLPLILILRNRLKYALTYREVIAILMQRHVLVDGKVRT
Sbjct: 142 MLDKLGGAFAPKPSSGPHKSRECLPLILILRNRLKYALTYREVIAILMQRHVLVDGKVRT 321
Query: 61 DKTYPAGFMDVVSIPKTNENFRLLYDTKGRFRLHSVRDDEAKFKLCKVRSVQFGQKGIPY 120
DKTYPAGFMDVVSIPKTNENFRLLYDTKGRFRLHSVRDDEAKFKLCKVRSVQFGQKGIPY
Sbjct: 322 DKTYPAGFMDVVSIPKTNENFRLLYDTKGRFRLHSVRDDEAKFKLCKVRSVQFGQKGIPY 501
Query: 121 LNTYDGRTIRYPDPLIKANDTIKLDLEQNKITDFIKFDVGNVVMVTGGRNRGRVGFIKNR 180
LNTYDGRTIRYPDPLIKANDTIKLDLEQNKITDFIKFDVGNVVMVTGGRNRGRVGFIKNR
Sbjct: 502 LNTYDGRTIRYPDPLIKANDTIKLDLEQNKITDFIKFDVGNVVMVTGGRNRGRVGFIKNR 681
Query: 181 KKHMGTFETIHVQDAAGHEFATRLGNVFTIGRGPKPWVSLPKGRGIKLTVIEEARKRMAA 240
KKHMGTFETIHVQDAAGHEFATRLGNVFTIGRGPKPWVSLPKGRGIKLTVIEEARKRMAA
Sbjct: 682 KKHMGTFETIHVQDAAGHEFATRLGNVFTIGRGPKPWVSLPKGRGIKLTVIEEARKRMAA 861
Query: 241 SQQAAAA 247
SQQAAAA
Sbjct: 862 SQQAAAA 882
>TC87437 homologue to GP|22138108|gb|AAM93434.1 40S ribosomal S4 protein
{Glycine max}, complete
Length = 1028
Score = 472 bits (1214), Expect = e-134
Identities = 233/246 (94%), Positives = 237/246 (95%)
Frame = +1
Query: 1 MLDKLGGAFAPKPSSGPHKSRECLPLILILRNRLKYALTYREVIAILMQRHVLVDGKVRT 60
MLDKLGGAFAPKPSSGPHKSRECLPLILILRNRLKYALTYREVIAILMQRHVLVDGKVRT
Sbjct: 142 MLDKLGGAFAPKPSSGPHKSRECLPLILILRNRLKYALTYREVIAILMQRHVLVDGKVRT 321
Query: 61 DKTYPAGFMDVVSIPKTNENFRLLYDTKGRFRLHSVRDDEAKFKLCKVRSVQFGQKGIPY 120
DKTYPAGFMDVVSIPKTNENFRLLYDTKGRFRLHSVRDDEAKFKLCKVRSVQFGQKGIPY
Sbjct: 322 DKTYPAGFMDVVSIPKTNENFRLLYDTKGRFRLHSVRDDEAKFKLCKVRSVQFGQKGIPY 501
Query: 121 LNTYDGRTIRYPDPLIKANDTIKLDLEQNKITDFIKFDVGNVVMVTGGRNRGRVGFIKNR 180
LNTYDGRTIRYPDPLIKANDTIKLDLE NKITDFIKFDVGNVVMVTGGRNRGRVG IKNR
Sbjct: 502 LNTYDGRTIRYPDPLIKANDTIKLDLENNKITDFIKFDVGNVVMVTGGRNRGRVGVIKNR 681
Query: 181 KKHMGTFETIHVQDAAGHEFATRLGNVFTIGRGPKPWVSLPKGRGIKLTVIEEARKRMAA 240
+KH GTFETIHVQDA+GHEFATRLGNVFTIG+G KPWVSLPKG+GIKLTVIEEARKR AA
Sbjct: 682 EKHKGTFETIHVQDASGHEFATRLGNVFTIGKGTKPWVSLPKGKGIKLTVIEEARKRQAA 861
Query: 241 SQQAAA 246
AA
Sbjct: 862 QNVTAA 879
>TC76614 homologue to GP|22138108|gb|AAM93434.1 40S ribosomal S4 protein
{Glycine max}, partial (98%)
Length = 1071
Score = 468 bits (1203), Expect = e-132
Identities = 230/247 (93%), Positives = 239/247 (96%)
Frame = +3
Query: 1 MLDKLGGAFAPKPSSGPHKSRECLPLILILRNRLKYALTYREVIAILMQRHVLVDGKVRT 60
MLDKLGGAFAPKPSSGPHKSRECLPLILILRNRLKYALTYREVIAILMQRHVLVDGKVRT
Sbjct: 111 MLDKLGGAFAPKPSSGPHKSRECLPLILILRNRLKYALTYREVIAILMQRHVLVDGKVRT 290
Query: 61 DKTYPAGFMDVVSIPKTNENFRLLYDTKGRFRLHSVRDDEAKFKLCKVRSVQFGQKGIPY 120
DKTYPAGFMDVVSIPKTNE+FRLLYDTKGRFRLHSVRDDEAKFKLCKVRSVQFGQKGIPY
Sbjct: 291 DKTYPAGFMDVVSIPKTNESFRLLYDTKGRFRLHSVRDDEAKFKLCKVRSVQFGQKGIPY 470
Query: 121 LNTYDGRTIRYPDPLIKANDTIKLDLEQNKITDFIKFDVGNVVMVTGGRNRGRVGFIKNR 180
LNTYDGRTIRYPDP+I+ANDTIKLDLE+NKI DFIKFDVGNVVMVTGG+NRGRVG IKNR
Sbjct: 471 LNTYDGRTIRYPDPVIRANDTIKLDLEENKIVDFIKFDVGNVVMVTGGKNRGRVGVIKNR 650
Query: 181 KKHMGTFETIHVQDAAGHEFATRLGNVFTIGRGPKPWVSLPKGRGIKLTVIEEARKRMAA 240
+KH GTFETIHVQDA GHEFATRL NVFTIG+G KPWVSLPKG+GIKLTVIEEARKR AA
Sbjct: 651 EKHKGTFETIHVQDATGHEFATRLVNVFTIGKGTKPWVSLPKGKGIKLTVIEEARKRAAA 830
Query: 241 SQQAAAA 247
+QQA AA
Sbjct: 831 AQQAVAA 851
>TC76613 homologue to GP|22138108|gb|AAM93434.1 40S ribosomal S4 protein
{Glycine max}, partial (98%)
Length = 1084
Score = 468 bits (1203), Expect = e-132
Identities = 230/247 (93%), Positives = 239/247 (96%)
Frame = +2
Query: 1 MLDKLGGAFAPKPSSGPHKSRECLPLILILRNRLKYALTYREVIAILMQRHVLVDGKVRT 60
MLDKLGGAFAPKPSSGPHKSRECLPLILILRNRLKYALTYREVIAILMQRHVLVDGKVRT
Sbjct: 131 MLDKLGGAFAPKPSSGPHKSRECLPLILILRNRLKYALTYREVIAILMQRHVLVDGKVRT 310
Query: 61 DKTYPAGFMDVVSIPKTNENFRLLYDTKGRFRLHSVRDDEAKFKLCKVRSVQFGQKGIPY 120
DKTYPAGFMDVVSIPKTNE+FRLLYDTKGRFRLHSVRDDEAKFKLCKVRSVQFGQKGIPY
Sbjct: 311 DKTYPAGFMDVVSIPKTNESFRLLYDTKGRFRLHSVRDDEAKFKLCKVRSVQFGQKGIPY 490
Query: 121 LNTYDGRTIRYPDPLIKANDTIKLDLEQNKITDFIKFDVGNVVMVTGGRNRGRVGFIKNR 180
LNTYDGRTIRYPDP+I+ANDTIKLDLE+NKI DFIKFDVGNVVMVTGG+NRGRVG IKNR
Sbjct: 491 LNTYDGRTIRYPDPVIRANDTIKLDLEENKIVDFIKFDVGNVVMVTGGKNRGRVGVIKNR 670
Query: 181 KKHMGTFETIHVQDAAGHEFATRLGNVFTIGRGPKPWVSLPKGRGIKLTVIEEARKRMAA 240
+KH GTFETIHVQDA GHEFATRL NVFTIG+G KPWVSLPKG+GIKLTVIEEARKR AA
Sbjct: 671 EKHKGTFETIHVQDATGHEFATRLVNVFTIGKGTKPWVSLPKGKGIKLTVIEEARKRAAA 850
Query: 241 SQQAAAA 247
+QQA AA
Sbjct: 851 AQQAVAA 871
>BQ146628 homologue to GP|22138108|gb 40S ribosomal S4 protein {Glycine max},
partial (47%)
Length = 716
Score = 236 bits (602), Expect = 6e-63
Identities = 114/130 (87%), Positives = 122/130 (93%)
Frame = +3
Query: 118 IPYLNTYDGRTIRYPDPLIKANDTIKLDLEQNKITDFIKFDVGNVVMVTGGRNRGRVGFI 177
IPYLNTYDGRTIRYPDP+I+ANDTIKLDLE+NKI DFIKFDVGNVVMVTGG+NRGRVG I
Sbjct: 9 IPYLNTYDGRTIRYPDPVIRANDTIKLDLEENKIVDFIKFDVGNVVMVTGGKNRGRVGVI 188
Query: 178 KNRKKHMGTFETIHVQDAAGHEFATRLGNVFTIGRGPKPWVSLPKGRGIKLTVIEEARKR 237
KNR+KH GTFETIHVQDA GHEFATRL NVFTIG+G KPWVSLPKG+GIKLTVIEEARKR
Sbjct: 189 KNREKHKGTFETIHVQDATGHEFATRLVNVFTIGKGTKPWVSLPKGKGIKLTVIEEARKR 368
Query: 238 MAASQQAAAA 247
AA+QQA AA
Sbjct: 369 AAAAQQAVAA 398
>BQ149101 similar to GP|22138108|gb| 40S ribosomal S4 protein {Glycine max},
partial (29%)
Length = 516
Score = 90.9 bits (224), Expect(3) = 4e-31
Identities = 42/53 (79%), Positives = 44/53 (82%)
Frame = +2
Query: 173 RVGFIKNRKKHMGTFETIHVQDAAGHEFATRLGNVFTIGRGPKPWVSLPKGRG 225
RVG IKNR KH GT TIHVQDA GHEF TRL NVFTIG+G KPWVSLPKG+G
Sbjct: 98 RVGVIKNRXKHKGTXXTIHVQDATGHEFPTRLVNVFTIGKGTKPWVSLPKGKG 256
Score = 48.9 bits (115), Expect(3) = 4e-31
Identities = 21/26 (80%), Positives = 24/26 (91%)
Frame = +1
Query: 147 EQNKITDFIKFDVGNVVMVTGGRNRG 172
++NKI DFIKFDVGN+VMVTGG NRG
Sbjct: 19 QENKIVDFIKFDVGNLVMVTGGXNRG 96
Score = 32.3 bits (72), Expect(3) = 4e-31
Identities = 15/20 (75%), Positives = 17/20 (85%)
Frame = +3
Query: 221 PKGRGIKLTVIEEARKRMAA 240
P+ RGIKLTVI+E RKR AA
Sbjct: 243 PRVRGIKLTVIQEXRKRAAA 302
>AL381307 similar to SP|O59950|RS4_Y 40S ribosomal protein S4 (S7). [Candida
lipolytica] {Yarrowia lipolytica}, partial (33%)
Length = 379
Score = 120 bits (300), Expect = 6e-28
Identities = 51/89 (57%), Positives = 70/89 (78%)
Frame = +2
Query: 154 FIKFDVGNVVMVTGGRNRGRVGFIKNRKKHMGTFETIHVQDAAGHEFATRLGNVFTIGRG 213
FIKF+VGN+ M+TGGRN GRVG I +R++H+G F+ +H++D+ +FATRL NVF IG G
Sbjct: 2 FIKFEVGNIAMITGGRNMGRVGIITHRERHVGGFDIVHIKDSVDRQFATRLINVFVIGEG 181
Query: 214 PKPWVSLPKGRGIKLTVIEEARKRMAASQ 242
KPW+SLPKG+G+KLT+ EE +R A +
Sbjct: 182 NKPWISLPKGKGVKLTIAEERDRRRALKE 268
>TC76625 similar to SP|O81363|RS4_PRUAR 40S ribosomal protein S4. [Apricot]
{Prunus armeniaca}, partial (13%)
Length = 1172
Score = 65.9 bits (159), Expect = 1e-11
Identities = 31/37 (83%), Positives = 34/37 (91%)
Frame = +2
Query: 211 GRGPKPWVSLPKGRGIKLTVIEEARKRMAASQQAAAA 247
G+G KPWVSLPKG+GIKLTVIEEARKR AA+QQA AA
Sbjct: 854 GKGTKPWVSLPKGKGIKLTVIEEARKRAAAAQQAVAA 964
>AW329812 homologue to GP|7340791|emb| ribosomal protein L23 {Spinacia
oleracea}, partial (49%)
Length = 510
Score = 55.1 bits (131), Expect = 2e-08
Identities = 23/25 (92%), Positives = 25/25 (100%)
Frame = +2
Query: 119 PYLNTYDGRTIRYPDPLIKANDTIK 143
PYLNTYDGRTIRYPDP+I+ANDTIK
Sbjct: 434 PYLNTYDGRTIRYPDPVIRANDTIK 508
>AW689115 weakly similar to GP|20873469|em putative metallophosphatase
{Lupinus luteus}, partial (16%)
Length = 636
Score = 29.6 bits (65), Expect = 1.1
Identities = 17/47 (36%), Positives = 26/47 (55%), Gaps = 2/47 (4%)
Frame = -2
Query: 122 NTYDGRTIRYPDPLIKA--NDTIKLDLEQNKITDFIKFDVGNVVMVT 166
N Y+ +I YP P IKA N++ K ++ + + KF G V+VT
Sbjct: 377 NQYERSSIEYPHPEIKAHNNESTKKKQRKSTVENVTKFK*GCYVIVT 237
>BQ144197 homologue to GP|1841930|emb| ROX protein {Mus musculus}, partial
(4%)
Length = 1170
Score = 28.5 bits (62), Expect = 2.5
Identities = 12/20 (60%), Positives = 14/20 (70%)
Frame = +2
Query: 167 GGRNRGRVGFIKNRKKHMGT 186
GG NRGR G +K R+KH T
Sbjct: 665 GGSNRGRKGSVKARQKHCRT 724
>TC79131 similar to GP|14031063|gb|AAK52092.1 WD-40 repeat protein
{Lycopersicon esculentum}, partial (71%)
Length = 1224
Score = 28.1 bits (61), Expect = 3.3
Identities = 11/25 (44%), Positives = 15/25 (60%)
Frame = -1
Query: 174 VGFIKNRKKHMGTFETIHVQDAAGH 198
+GF R+KH+ +H QDAA H
Sbjct: 1035 IGFHHERRKHL*HLGNVHAQDAAKH 961
>TC90027 similar to GP|4206789|gb|AAD11809.1| syntaxin-related protein
At-SYR1 {Arabidopsis thaliana}, partial (82%)
Length = 1362
Score = 28.1 bits (61), Expect = 3.3
Identities = 14/39 (35%), Positives = 21/39 (52%)
Frame = +3
Query: 74 IPKTNENFRLLYDTKGRFRLHSVRDDEAKFKLCKVRSVQ 112
+ KTNE + ++D KG L S D+E L K + V+
Sbjct: 237 LSKTNEQTKTVHDAKGVKELRSRMDEEVSAALKKAKMVK 353
>TC87302 similar to GP|19911199|dbj|BAB86926. glucosyltransferase-8 {Vigna
angularis}, partial (53%)
Length = 1787
Score = 27.3 bits (59), Expect = 5.6
Identities = 11/35 (31%), Positives = 20/35 (56%)
Frame = +1
Query: 210 IGRGPKPWVSLPKGRGIKLTVIEEARKRMAASQQA 244
+G G + W+ + G +K VIE+A +R+ +A
Sbjct: 1243 VGVGVQTWIGMGGGEPVKKDVIEKAVRRIMVGDEA 1347
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.322 0.140 0.413
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 6,529,607
Number of Sequences: 36976
Number of extensions: 79860
Number of successful extensions: 366
Number of sequences better than 10.0: 28
Number of HSP's better than 10.0 without gapping: 366
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 366
length of query: 247
length of database: 9,014,727
effective HSP length: 94
effective length of query: 153
effective length of database: 5,538,983
effective search space: 847464399
effective search space used: 847464399
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 57 (26.6 bits)
Medicago: description of AC146554.3