BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146552.9 - phase: 0 /pseudo
(454 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC80739 AKIN betagamma [Medicago truncatula] 334 e-118
AW774494 similar to GP|16612255|gb At1g09020/F7G19_11 {Arabidops... 189 1e-48
TC87494 similar to GP|5702015|emb|CAB52141.1 GAL83 protein {Sola... 49 4e-06
TC88535 weakly similar to GP|6686784|emb|CAB64719.1 AKIN beta2 {... 47 2e-05
BF003903 similar to GP|15010688|gb AT5g39790/MKM21_80 {Arabidops... 45 8e-05
BE318977 similar to GP|21593717|gb| AKIN beta1 {Arabidopsis thal... 31 0.002
TC87770 similar to GP|14970762|emb|CAC44460. protein tyrosine ph... 32 0.39
TC80096 weakly similar to GP|22202703|dbj|BAC07361. contains EST... 30 2.5
BE326165 similar to GP|9758330|dbj| contains similarity to nucle... 28 9.6
>TC80739 AKIN betagamma [Medicago truncatula]
Length = 1535
Score = 334 bits (856), Expect(2) = e-118
Identities = 204/392 (52%), Positives = 251/392 (63%), Gaps = 21/392 (5%)
Frame = +3
Query: 84 HDEHTPHITGDYGIVNTVLLATDPFV--PVLPPDIVSGSNMDVDNETFQRVVRLTDGTLS 141
+DE P + G+YGIVNT+ L +P + +L + S S+M+VDN+ F +
Sbjct: 372 YDEQQPFVNGNYGIVNTIYLVREPDILPAILSAETSSRSHMEVDNDVFGH---------A 524
Query: 142 EVMPRISDVDVQTSRQRISTYLSMRTAYELLPESGKVVTLDVDLPVKQAFHILHEQGIPM 201
E PR+S D++ SR+RIS +LS TAY+LLPESGKV+ LDV+LPVKQAFH+L+EQ + M
Sbjct: 525 EANPRMSPSDLEVSRRRISKFLSEHTAYDLLPESGKVIALDVNLPVKQAFHVLYEQDVSM 704
Query: 202 APLWDFCKGQFVGVLSVLDFILILRELGNHGSNLT-EEELETHTISAWKEGKWTLFSRR- 259
APLWDFCK QFVGVLS +DFILIL+ELG HGS+ L KEGK RR
Sbjct: 705 APLWDFCKSQFVGVLSAMDFILILKELGTHGSHFD*RTTLRLIL*QPGKEGKSK--QRRA 878
Query: 260 -----------FIHAGPSDNLKDVALKILQNGISTVPIIHSSSADGSFPQLLHLASLSGI 308
F+HAGP + LKDVALK+LQN +STVPII S DGSFPQLLHLASLSGI
Sbjct: 879 LDNNEGSNPHCFVHAGPKECLKDVALKVLQNKVSTVPII--SLEDGSFPQLLHLASLSGI 1052
Query: 309 LRCILGVVLVHCPYFNFQF----VQFLWAHGCPKLGRQIAGL*QH*DQMLLLLQP*I-Y* 363
L+CI C +F + L P LG + + Q L+ L+P
Sbjct: 1053LKCI-------CRHFEHSAGSLPILQLPIASIP-LGTWVPNVGDPNGQPLIRLRPNASLG 1208
Query: 364 FKFNIFFAAQVSSIPIVDESDSLLDIYCRSDITALAKDRAYTHINLDEMTVHQALQLSQD 423
++F A+VSSIPIVDE+DSLLDIY RSDITALAKD+AY I+LDE +HQAL L QD
Sbjct: 1209DALSMFVQAKVSSIPIVDENDSLLDIYSRSDITALAKDKAYARISLDETNIHQALILGQD 1388
Query: 424 AFNP-NESRSQRCQMCLRTDSLHKVMERLANP 454
A +P + RC MCLR+DSLHKVMERLA P
Sbjct: 1389ANSPYGLNNGHRCHMCLRSDSLHKVMERLAKP 1484
Score = 111 bits (277), Expect(2) = e-118
Identities = 48/67 (71%), Positives = 57/67 (84%)
Frame = +2
Query: 17 AAGTVLIPVRFVWPYGGRTVYLSGSFTRWSELLQMSPVEGCPTVFQVIHNLAPGYHQYKF 76
++G +LIP RFVWPYGG VYL GSFTRWSE + MSP+EGCP+VFQVI +L PGYHQ+KF
Sbjct: 170 SSGPILIPKRFVWPYGGTRVYLIGSFTRWSEHIPMSPMEGCPSVFQVICSLMPGYHQFKF 349
Query: 77 FVDGEWR 83
VDG+WR
Sbjct: 350 NVDGQWR 370
>AW774494 similar to GP|16612255|gb At1g09020/F7G19_11 {Arabidopsis
thaliana}, partial (25%)
Length = 752
Score = 189 bits (481), Expect = 1e-48
Identities = 96/141 (68%), Positives = 112/141 (79%), Gaps = 12/141 (8%)
Frame = +3
Query: 159 ISTYLSMRTAYELLPESGKVVTLDVDLPVKQAFHILHEQGIPMAPLWDFCKGQFVGVLSV 218
IS +LS TAY+LLPESGKV+ LDV+LPVKQAFH+L+EQ + MAPLWDFCK QFVGVLS
Sbjct: 297 ISKFLSEHTAYDLLPESGKVIALDVNLPVKQAFHVLYEQDVSMAPLWDFCKSQFVGVLSA 476
Query: 219 LDFILILRELGNHGSNLTEEELETHTISAWKEGKWTLFSRR------------FIHAGPS 266
+DFILIL+ELG HGS+LTEE+LETHTI+AWKEGK RR F+HAGP
Sbjct: 477 MDFILILKELGTHGSHLTEEQLETHTIAAWKEGKSK--QRRALDNNEGSNPHCFVHAGPK 650
Query: 267 DNLKDVALKILQNGISTVPII 287
+ LKDVALK+LQN +STVPII
Sbjct: 651 ECLKDVALKVLQNKVSTVPII 713
>TC87494 similar to GP|5702015|emb|CAB52141.1 GAL83 protein {Solanum
tuberosum}, partial (81%)
Length = 1415
Score = 48.9 bits (115), Expect = 4e-06
Identities = 30/96 (31%), Positives = 47/96 (48%), Gaps = 2/96 (2%)
Frame = +3
Query: 23 IPVRFVWPYGGRTVYLSGSFTRWS--ELLQMSPVEGCPTVFQVIHNLAPGYHQYKFFVDG 80
IPV W YGG +V + GS+ W+ ++LQ + ++ L G Y+F VDG
Sbjct: 480 IPVMITWNYGGNSVAVEGSWDNWASRKVLQRGGKD-----HSILIVLPSGIFHYRFIVDG 644
Query: 81 EWRHDEHTPHITGDYGIVNTVLLATDPFVPVLPPDI 116
E R+ P++ + G V +L D +VP P +
Sbjct: 645 EQRYIPDLPYVADEMGNVCNLLDVND-YVPENPESV 749
>TC88535 weakly similar to GP|6686784|emb|CAB64719.1 AKIN beta2 {Arabidopsis
thaliana}, partial (30%)
Length = 759
Score = 47.0 bits (110), Expect = 2e-05
Identities = 26/86 (30%), Positives = 40/86 (46%)
Frame = +1
Query: 25 VRFVWPYGGRTVYLSGSFTRWSELLQMSPVEGCPTVFQVIHNLAPGYHQYKFFVDGEWRH 84
VR W +GG V ++GS+ W + + V F ++ L + Y+F VDG+W H
Sbjct: 259 VRITWIHGGTNVSIAGSWNNWETVEALLRVG---QHFVIVKTLPISIYYYRFIVDGQWTH 429
Query: 85 DEHTPHITGDYGIVNTVLLATDPFVP 110
P D G V +L D ++P
Sbjct: 430 APEFPSDLDDSGYVYNILDLQD-YIP 504
>BF003903 similar to GP|15010688|gb AT5g39790/MKM21_80 {Arabidopsis
thaliana}, partial (37%)
Length = 441
Score = 44.7 bits (104), Expect = 8e-05
Identities = 32/93 (34%), Positives = 45/93 (47%), Gaps = 1/93 (1%)
Frame = +3
Query: 12 VGGVVAAGTVLIPVRFVWPYGGRTVYLSGSFTRWSELLQMSP-VEGCPTVFQVIHNLAPG 70
+ GV AA + + V W +V + GSF WS+ +SP G T F L PG
Sbjct: 48 IKGVDAAQSKEVSV--FWVGMAESVQVMGSFDGWSQGEHLSPEYTGSYTRFSTTLMLRPG 221
Query: 71 YHQYKFFVDGEWRHDEHTPHITGDYGIVNTVLL 103
++ KF VDGEW P +TG+ N +L+
Sbjct: 222 RYEIKFLVDGEWHLSTELP-VTGEGLTKNNLLI 317
>BE318977 similar to GP|21593717|gb| AKIN beta1 {Arabidopsis thaliana},
partial (28%)
Length = 539
Score = 30.8 bits (68), Expect(2) = 0.002
Identities = 11/24 (45%), Positives = 15/24 (61%)
Frame = +2
Query: 23 IPVRFVWPYGGRTVYLSGSFTRWS 46
IPV W YGG +V + GS+ W+
Sbjct: 305 IPVMITWNYGGNSVAVEGSWDNWA 376
Score = 28.5 bits (62), Expect(2) = 0.002
Identities = 13/37 (35%), Positives = 19/37 (51%)
Frame = +3
Query: 70 GYHQYKFFVDGEWRHDEHTPHITGDYGIVNTVLLATD 106
G Y+F VDGE R+ P++ + G V +L D
Sbjct: 381 GIFHYRFIVDGEQRYIPDLPYVADEMGNVCNLLDVND 491
>TC87770 similar to GP|14970762|emb|CAC44460. protein tyrosine phosphatase
{Lycopersicon esculentum}, partial (82%)
Length = 1714
Score = 32.3 bits (72), Expect = 0.39
Identities = 20/80 (25%), Positives = 37/80 (46%), Gaps = 1/80 (1%)
Frame = +1
Query: 4 PSMDSARDVGGVVAAGTVLIPVRFVWPYGG-RTVYLSGSFTRWSELLQMSPVEGCPTVFQ 62
P +D+ + + G PV W + TV +SG W + + ++ + + F
Sbjct: 1009 PKLDAIKSATADILTGLSKKPVTLSWGHRNCSTVEISGLDIGWGQRVPLNFDDKQGSWF- 1185
Query: 63 VIHNLAPGYHQYKFFVDGEW 82
+ + G ++YK+ VDGEW
Sbjct: 1186 LKKEMFEGRYEYKYIVDGEW 1245
>TC80096 weakly similar to GP|22202703|dbj|BAC07361. contains ESTs
C26612(C12696) AU108106(C12696)~similar to Arabidopsis
thaliana chromosome 5, partial (12%)
Length = 931
Score = 29.6 bits (65), Expect = 2.5
Identities = 17/65 (26%), Positives = 34/65 (52%), Gaps = 2/65 (3%)
Frame = +1
Query: 271 DVALKILQNGISTVPIIHSSSADGSFPQLLHLASLS--GILRCILGVVLVHCPYFNFQFV 328
DV +K+++N + + P+IHS+ L +S+S ++ ++ H YFNF
Sbjct: 148 DVEMKLIKN*LISFPLIHST-------VFLFFSSISRLSLIVSFSSILTFHIIYFNFNQF 306
Query: 329 QFLWA 333
F+++
Sbjct: 307 SFIFS 321
>BE326165 similar to GP|9758330|dbj| contains similarity to nuclear protein
95~gene_id:MIJ24.3 {Arabidopsis thaliana}, partial (6%)
Length = 572
Score = 27.7 bits (60), Expect = 9.6
Identities = 13/28 (46%), Positives = 16/28 (56%)
Frame = -1
Query: 280 GISTVPIIHSSSADGSFPQLLHLASLSG 307
G P I SSS +GS+ QL H S+ G
Sbjct: 212 GFEDPPTISSSSVEGSYEQLGHFPSIIG 129
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.328 0.142 0.446
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 14,782,359
Number of Sequences: 36976
Number of extensions: 222044
Number of successful extensions: 1274
Number of sequences better than 10.0: 18
Number of HSP's better than 10.0 without gapping: 1252
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1267
length of query: 454
length of database: 9,014,727
effective HSP length: 99
effective length of query: 355
effective length of database: 5,354,103
effective search space: 1900706565
effective search space used: 1900706565
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.8 bits)
S2: 60 (27.7 bits)
Medicago: description of AC146552.9