BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146549.14 + phase: 0 /pseudo/partial
(221 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
CB065382 similar to PIR|T09671|T096 RPE15 protein - alfalfa (fra... 61 3e-10
BQ150350 32 0.15
TC86583 similar to GP|21593999|gb|AAM65917.1 ATP phosphoribosyl ... 32 0.25
BG586393 similar to GP|11994408|dbj CTP synthase {Arabidopsis th... 29 1.3
TC81694 homologue to PIR|S61505|S61505 UDPglucose--starch glucos... 29 1.6
TC86431 similar to SP|Q8W425|PSD6_ORYSA 26S proteasome non-ATPas... 28 2.1
TC76496 weakly similar to PIR|S78099|S78099 furostanol glycoside... 28 2.8
TC79570 similar to PIR|F86427|F86427 auxin response factor 6 (AR... 28 2.8
TC83283 similar to GP|14334984|gb|AAK59669.1 unknown protein {Ar... 28 2.8
TC87536 similar to GP|21553686|gb|AAM62779.1 unknown {Arabidopsi... 28 2.8
TC88861 weakly similar to GP|17027283|gb|AAL34137.1 putative pro... 28 2.8
TC85910 homologue to SP|P08926|RUBA_PEA RuBisCO subunit binding-... 28 3.6
TC80288 weakly similar to PIR|T06029|T06029 hypothetical protein... 28 3.6
TC76683 similar to SP|Q41140|PFPA_RICCO Pyrophosphate--fructose ... 28 3.6
TC78843 weakly similar to GP|8843897|dbj|BAA97423.1 1-aminocyclo... 25 4.1
TC77483 similar to GP|15450373|gb|AAK96480.1 AT4g32480/F8B4_180 ... 27 4.8
TC77485 similar to GP|15450373|gb|AAK96480.1 AT4g32480/F8B4_180 ... 27 4.8
TC82881 similar to GP|15216026|emb|CAC51423. amino acid permease... 27 4.8
TC84055 similar to PIR|E86216|E86216 protein T23G18.10 [imported... 27 4.8
TC77484 similar to GP|15450373|gb|AAK96480.1 AT4g32480/F8B4_180 ... 27 4.8
>CB065382 similar to PIR|T09671|T096 RPE15 protein - alfalfa (fragment),
partial (9%)
Length = 624
Score = 61.2 bits (147), Expect = 3e-10
Identities = 33/57 (57%), Positives = 38/57 (65%)
Frame = -3
Query: 164 RFNNNNKISKLGLLSLRYRCYMLSCFQLYSREGIVQLDRVSPHLIRCLQGSGLISNV 220
RF NN+ ++L L L Y+CYMLS +LY EG Q DR S HLIR L GSGL SNV
Sbjct: 517 RFTNNDSSNRLDLPFL*YQCYMLSSSRLYFSEGTAQSDRGSLHLIRYLPGSGLTSNV 347
>BQ150350
Length = 1064
Score = 32.3 bits (72), Expect = 0.15
Identities = 16/33 (48%), Positives = 21/33 (63%)
Frame = -2
Query: 110 LLLLSMQLKCLRHTHICRILSIRSSHRFIISTL 142
L LL++QL LRH I + I + H +IISTL
Sbjct: 952 LRLLNLQLHLLRHHFIATVFRITNIHSYIISTL 854
>TC86583 similar to GP|21593999|gb|AAM65917.1 ATP phosphoribosyl transferase
{Arabidopsis thaliana}, partial (82%)
Length = 1507
Score = 31.6 bits (70), Expect = 0.25
Identities = 29/113 (25%), Positives = 41/113 (35%)
Frame = -2
Query: 102 LEPVNQWLLLLLSMQLKCLRHTHICRILSIRSSHRFIISTLCHRANLRFLSMPSLNRYNI 161
L+P +WL C +HIC + + + + H + S S
Sbjct: 972 LQPSQRWLAQYSLSHFLCTASSHICSDRELPHGPQMCLQSFKHLMSCFKYSFSS------ 811
Query: 162 NCRFNNNNKISKLGLLSLRYRCYMLSCFQLYSREGIVQLDRVSPHLIRCLQGS 214
N RF NK S S + + + FQ S VQ +PH CLQ S
Sbjct: 810 NHRFPARNKSSLAFQNSSTFNFFQVIFFQCSSTTYKVQDSISNPHRRSCLQCS 652
>BG586393 similar to GP|11994408|dbj CTP synthase {Arabidopsis thaliana},
partial (9%)
Length = 668
Score = 29.3 bits (64), Expect = 1.3
Identities = 13/37 (35%), Positives = 18/37 (48%)
Frame = +1
Query: 156 LNRYNINCRFNNNNKISKLGLLSLRYRCYMLSCFQLY 192
L +INC NNNN G Y C +L+C ++
Sbjct: 79 LRNNDINCSNNNNNNFGNYG-----YECRLLNCLAIH 174
>TC81694 homologue to PIR|S61505|S61505 UDPglucose--starch
glucosyltransferase (EC 2.4.1.11) isoform II precursor -
garden pea, partial (23%)
Length = 836
Score = 28.9 bits (63), Expect = 1.6
Identities = 19/64 (29%), Positives = 30/64 (46%), Gaps = 3/64 (4%)
Frame = +2
Query: 135 HRFIISTLCHRANLRFLSMPSLNRYNINCRFNNNNKISKLGLLSLRY---RCYMLSCFQL 191
HR ++ T+ +FL +P N N +I LG+ S C+ML+CF++
Sbjct: 455 HRILVGTMLLNNTRKFLLLP-----------NTNGEI--LGMYSTFVV*STCFMLNCFKV 595
Query: 192 YSRE 195
Y E
Sbjct: 596 YETE 607
>TC86431 similar to SP|Q8W425|PSD6_ORYSA 26S proteasome non-ATPase regulatory
subunit 6, partial (97%)
Length = 1517
Score = 28.5 bits (62), Expect = 2.1
Identities = 10/36 (27%), Positives = 21/36 (57%)
Frame = -1
Query: 186 LSCFQLYSREGIVQLDRVSPHLIRCLQGSGLISNVT 221
L C ++ G+++ PH+++C+Q LI +V+
Sbjct: 1406 LGCSLIWKSNGLLKCHASFPHIVKCIQQMSLILSVS 1299
>TC76496 weakly similar to PIR|S78099|S78099 furostanol glycoside
26-O-beta-glucosidase F26G - Costus speciosus, partial
(35%)
Length = 1879
Score = 28.1 bits (61), Expect = 2.8
Identities = 13/37 (35%), Positives = 21/37 (56%), Gaps = 4/37 (10%)
Frame = -2
Query: 177 LSLRYRCYML----SCFQLYSREGIVQLDRVSPHLIR 209
L R +C +L SC Y+ E I+++D + PH+ R
Sbjct: 1653 LVYRIKCQILFVPFSCLCRYTFETIIKIDMIKPHMFR 1543
>TC79570 similar to PIR|F86427|F86427 auxin response factor 6 (ARF6)
[imported] - Arabidopsis thaliana, partial (15%)
Length = 1143
Score = 28.1 bits (61), Expect = 2.8
Identities = 27/90 (30%), Positives = 41/90 (45%), Gaps = 3/90 (3%)
Frame = +3
Query: 121 RHTHICRILSIRSSHRFIISTLCHRANLRFLSMPSLNRYNINCRFNNN-NKISKLGLLSL 179
+H + SI S+H I +C+ ++ S S N NI+ NNN N +KL +++
Sbjct: 510 KHNNSSSFFSI-STHSLIKIIICNSSSSNSSSNSSNNSSNISSSNNNNHNSNNKL*IIN- 683
Query: 180 RYRCYMLS--CFQLYSREGIVQLDRVSPHL 207
RC M S C L+ + Q P L
Sbjct: 684 --RCQMPSPQCLSLFPHLHLNQPSPCKPFL 767
>TC83283 similar to GP|14334984|gb|AAK59669.1 unknown protein {Arabidopsis
thaliana}, partial (49%)
Length = 861
Score = 28.1 bits (61), Expect = 2.8
Identities = 17/64 (26%), Positives = 33/64 (51%)
Frame = -1
Query: 127 RILSIRSSHRFIISTLCHRANLRFLSMPSLNRYNINCRFNNNNKISKLGLLSLRYRCYML 186
+IL +SH I T+ F+ + + ++ NC F ++K++ + LL + CY L
Sbjct: 354 KILGCHTSHHHKIFTI-------FIGL--VLKWRTNCIFTGSSKLAYVLLLRIYICCYRL 202
Query: 187 SCFQ 190
C++
Sbjct: 201 ICWR 190
>TC87536 similar to GP|21553686|gb|AAM62779.1 unknown {Arabidopsis thaliana},
partial (94%)
Length = 1358
Score = 28.1 bits (61), Expect = 2.8
Identities = 17/52 (32%), Positives = 22/52 (41%), Gaps = 6/52 (11%)
Frame = +1
Query: 95 LKWGWYQL------EPVNQWLLLLLSMQLKCLRHTHICRILSIRSSHRFIIS 140
L WGW L E LLL M+L C H C I+ + + F+ S
Sbjct: 1015 LVWGWLDLR*RLDSEEEKMCNKLLLIMKLSCYPHHLFCLIMYFKFDYLFLSS 1170
>TC88861 weakly similar to GP|17027283|gb|AAL34137.1 putative protein kinase
{Oryza sativa (japonica cultivar-group)}, partial (34%)
Length = 1847
Score = 28.1 bits (61), Expect = 2.8
Identities = 12/30 (40%), Positives = 16/30 (53%)
Frame = +3
Query: 119 CLRHTHICRILSIRSSHRFIISTLCHRANL 148
C+RH CR + +S R II T R +L
Sbjct: 1431 CIRHCQACRFWGVENSDREII*TFAERKSL 1520
>TC85910 homologue to SP|P08926|RUBA_PEA RuBisCO subunit binding-protein
alpha subunit chloroplast precursor, complete
Length = 1997
Score = 27.7 bits (60), Expect = 3.6
Identities = 17/45 (37%), Positives = 19/45 (41%)
Frame = -1
Query: 114 SMQLKCLRHTHICRILSIRSSHRFIISTLCHRANLRFLSMPSLNR 158
S LK + C I S HR IST CH + PS NR
Sbjct: 626 SQLLKLFHQSRYCFINSFLDGHR--IST*CHTEKSKLNDFPSQNR 498
>TC80288 weakly similar to PIR|T06029|T06029 hypothetical protein T28I19.100
- Arabidopsis thaliana, partial (14%)
Length = 1460
Score = 27.7 bits (60), Expect = 3.6
Identities = 20/67 (29%), Positives = 33/67 (48%), Gaps = 1/67 (1%)
Frame = +1
Query: 53 RWSLWEPVLRKPSGMESLCLRKLNLLRAHRRGMVMDTTRRKKLKWGWYQL-EPVNQWLLL 111
R LW + RK + R++ R +RG+ + TTR KL +L P+ W ++
Sbjct: 685 RMRLW--MKRKTKKKRVMMRRRMKTRRMRKRGV*LKTTRIMKLGKNITRLMMPLVLWHMI 858
Query: 112 LLSMQLK 118
L+ + LK
Sbjct: 859 LMKLALK 879
>TC76683 similar to SP|Q41140|PFPA_RICCO Pyrophosphate--fructose 6-phosphate
1-phosphotransferase alpha subunit (EC 2.7.1.90) (PFP),
partial (98%)
Length = 2413
Score = 27.7 bits (60), Expect = 3.6
Identities = 13/30 (43%), Positives = 17/30 (56%)
Frame = -2
Query: 90 TRRKKLKWGWYQLEPVNQWLLLLLSMQLKC 119
TR KLKW W + V++ +LS QL C
Sbjct: 1722 TRTIKLKWAWISINIVHKKACRILS*QLIC 1633
>TC78843 weakly similar to GP|8843897|dbj|BAA97423.1
1-aminocyclopropane-1-carboxylate oxidase {Arabidopsis
thaliana}, partial (70%)
Length = 1240
Score = 25.4 bits (54), Expect(2) = 4.1
Identities = 10/24 (41%), Positives = 15/24 (61%)
Frame = -3
Query: 113 LSMQLKCLRHTHICRILSIRSSHR 136
L +QL C++H+ IC +L HR
Sbjct: 926 LHLQLLCVQHS*ICHLL*AAEDHR 855
Score = 20.4 bits (41), Expect(2) = 4.1
Identities = 8/16 (50%), Positives = 11/16 (68%)
Frame = -1
Query: 170 KISKLGLLSLRYRCYM 185
KI+ + S RYRCY+
Sbjct: 865 KITDINNESSRYRCYI 818
>TC77483 similar to GP|15450373|gb|AAK96480.1 AT4g32480/F8B4_180
{Arabidopsis thaliana}, partial (34%)
Length = 818
Score = 27.3 bits (59), Expect = 4.8
Identities = 10/26 (38%), Positives = 16/26 (61%)
Frame = -3
Query: 183 CYMLSCFQLYSREGIVQLDRVSPHLI 208
CY++S L+ +G+ D SPHL+
Sbjct: 630 CYLISLLFLHKPQGVSSQDGYSPHLL 553
>TC77485 similar to GP|15450373|gb|AAK96480.1 AT4g32480/F8B4_180
{Arabidopsis thaliana}, partial (27%)
Length = 866
Score = 27.3 bits (59), Expect = 4.8
Identities = 10/26 (38%), Positives = 16/26 (61%)
Frame = -1
Query: 183 CYMLSCFQLYSREGIVQLDRVSPHLI 208
CY++S L+ +G+ D SPHL+
Sbjct: 296 CYLISLLFLHKPQGVSSQDGYSPHLL 219
>TC82881 similar to GP|15216026|emb|CAC51423. amino acid permease AAP1
{Vicia faba var. minor}, partial (28%)
Length = 1132
Score = 27.3 bits (59), Expect = 4.8
Identities = 19/65 (29%), Positives = 31/65 (47%), Gaps = 1/65 (1%)
Frame = -1
Query: 116 QLKCLRHTHICRILSIRSSHRFIISTLCHRANLRFLSMP-SLNRYNINCRFNNNNKISKL 174
QL C+ HTH R +R+ H+ ++ F+ ++ R C NNNNK+
Sbjct: 523 QLDCISHTHTHR--DLRTRHQRNKRDFYNQLRTIFIYFYYTIIRIGQICNQNNNNKVKLD 350
Query: 175 GLLSL 179
G+ +L
Sbjct: 349 GISAL 335
>TC84055 similar to PIR|E86216|E86216 protein T23G18.10 [imported] -
Arabidopsis thaliana, partial (18%)
Length = 851
Score = 27.3 bits (59), Expect = 4.8
Identities = 13/36 (36%), Positives = 18/36 (49%)
Frame = +2
Query: 119 CLRHTHICRILSIRSSHRFIISTLCHRANLRFLSMP 154
CL+ ++C LSI + R I T H +F MP
Sbjct: 26 CLKFLYVCLCLSISAQTRVITFTRVHSTTNQFSWMP 133
>TC77484 similar to GP|15450373|gb|AAK96480.1 AT4g32480/F8B4_180
{Arabidopsis thaliana}, partial (27%)
Length = 1238
Score = 27.3 bits (59), Expect = 4.8
Identities = 10/26 (38%), Positives = 16/26 (61%)
Frame = -3
Query: 183 CYMLSCFQLYSREGIVQLDRVSPHLI 208
CY++S L+ +G+ D SPHL+
Sbjct: 801 CYLISLLFLHKPQGVSSQDGYSPHLL 724
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.340 0.147 0.491
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 9,005,312
Number of Sequences: 36976
Number of extensions: 158688
Number of successful extensions: 1567
Number of sequences better than 10.0: 50
Number of HSP's better than 10.0 without gapping: 1555
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1565
length of query: 221
length of database: 9,014,727
effective HSP length: 92
effective length of query: 129
effective length of database: 5,612,935
effective search space: 724068615
effective search space used: 724068615
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 39 (21.9 bits)
S2: 56 (26.2 bits)
Medicago: description of AC146549.14