BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146548.2 + phase: 0
(203 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC88472 similar to GP|19423958|gb|AAL87269.1 unknown protein {Ar... 403 e-113
TC89281 similar to GP|11994197|dbj|BAB01300. emb|CAA18500.1~gene... 91 3e-19
BG583940 71 3e-13
TC86370 similar to GP|15451010|gb|AAK96776.1 Unknown protein {Ar... 68 3e-12
TC89847 similar to GP|20127030|gb|AAM10939.1 putative bHLH trans... 68 3e-12
TC86369 similar to GP|15451010|gb|AAK96776.1 Unknown protein {Ar... 67 5e-12
TC86368 similar to GP|15451010|gb|AAK96776.1 Unknown protein {Ar... 66 1e-11
TC83223 similar to GP|8777436|dbj|BAA97026.1 gb|AAC78547.1~gene_... 41 3e-04
BQ143793 weakly similar to GP|6523547|emb| hydroxyproline-rich g... 37 0.005
TC77200 similar to GP|22597162|gb|AAN03468.1 bZIP transcription ... 35 0.020
AW981132 similar to GP|4457221|gb|A putative bZIP DNA-binding pr... 34 0.034
TC91130 homologue to GP|11245494|gb|AAG33640.1 SPATULA {Arabidop... 33 0.099
TC85773 weakly similar to GP|9650828|emb|CAC00658.1 common plant... 33 0.099
TC85772 weakly similar to GP|9650828|emb|CAC00658.1 common plant... 33 0.099
BG453035 weakly similar to GP|10334495|emb putative extracellula... 31 0.38
BF646001 homologue to GP|20303561|gb| putative kinase {Oryza sat... 30 0.49
AJ501172 similar to GP|20466324|gb| unknown protein {Arabidopsis... 30 0.64
TC92109 similar to GP|19683031|gb|AAL92653.1 HYPOTHETICAL 31.4 K... 30 0.64
AW586676 similar to GP|806720|gb|AA arabinogalactan-protein prec... 30 0.84
TC89647 similar to GP|20258923|gb|AAM14177.1 unknown protein {Ar... 29 1.1
>TC88472 similar to GP|19423958|gb|AAL87269.1 unknown protein {Arabidopsis
thaliana}, partial (46%)
Length = 1019
Score = 403 bits (1036), Expect = e-113
Identities = 203/203 (100%), Positives = 203/203 (100%)
Frame = +2
Query: 1 MKQGKVPKRIHKAEREKMKREHLNELFLDLANALDLSEPNNGKASILIEASRLLKDLLCQ 60
MKQGKVPKRIHKAEREKMKREHLNELFLDLANALDLSEPNNGKASILIEASRLLKDLLCQ
Sbjct: 275 MKQGKVPKRIHKAEREKMKREHLNELFLDLANALDLSEPNNGKASILIEASRLLKDLLCQ 454
Query: 61 IQSLKKENVSLLSESHYVTMEKNELKEENSSLETQIEKLQGEIQARIAQSKPDLNAPPQL 120
IQSLKKENVSLLSESHYVTMEKNELKEENSSLETQIEKLQGEIQARIAQSKPDLNAPPQL
Sbjct: 455 IQSLKKENVSLLSESHYVTMEKNELKEENSSLETQIEKLQGEIQARIAQSKPDLNAPPQL 634
Query: 121 ELESPEQTTFSGQSFQLPTVDPTLQQGPTVLVVPFRPDLQAAYPAPTEITPNPPLVISKP 180
ELESPEQTTFSGQSFQLPTVDPTLQQGPTVLVVPFRPDLQAAYPAPTEITPNPPLVISKP
Sbjct: 635 ELESPEQTTFSGQSFQLPTVDPTLQQGPTVLVVPFRPDLQAAYPAPTEITPNPPLVISKP 814
Query: 181 HARYPTPADSWPSQLLGEQPTSS 203
HARYPTPADSWPSQLLGEQPTSS
Sbjct: 815 HARYPTPADSWPSQLLGEQPTSS 883
>TC89281 similar to GP|11994197|dbj|BAB01300.
emb|CAA18500.1~gene_id:MPN9.10~similar to unknown
protein {Arabidopsis thaliana}, partial (43%)
Length = 733
Score = 90.9 bits (224), Expect = 3e-19
Identities = 46/105 (43%), Positives = 67/105 (63%)
Frame = +1
Query: 8 KRIHKAEREKMKREHLNELFLDLANALDLSEPNNGKASILIEASRLLKDLLCQIQSLKKE 67
++ KA+REK++R+ LNE FL+L N LD P N KA+IL + +LLKDL Q+ LK E
Sbjct: 211 RKTQKADREKLRRDRLNEQFLELGNILDPDRPKNDKATILGDTVQLLKDLSSQVSKLKDE 390
Query: 68 NVSLLSESHYVTMEKNELKEENSSLETQIEKLQGEIQARIAQSKP 112
L ES ++ EKN+L+EE +SL++ IE L + Q ++ P
Sbjct: 391 YTMLNEESRELSQEKNDLREEKASLKSDIENLNNQYQLQLRTMYP 525
>BG583940
Length = 787
Score = 71.2 bits (173), Expect = 3e-13
Identities = 34/50 (68%), Positives = 38/50 (76%), Gaps = 2/50 (4%)
Frame = -3
Query: 150 VLVVPFRPDLQAAYPAP--TEITPNPPLVISKPHARYPTPADSWPSQLLG 197
V V+PF PDLQA +PAP TEITP P V +K + RYPTPAD WPSQLLG
Sbjct: 518 VSVIPFLPDLQATFPAPYVTEITPKPTPVTNKQYVRYPTPADLWPSQLLG 369
>TC86370 similar to GP|15451010|gb|AAK96776.1 Unknown protein {Arabidopsis
thaliana}, partial (80%)
Length = 1050
Score = 67.8 bits (164), Expect = 3e-12
Identities = 39/108 (36%), Positives = 65/108 (60%), Gaps = 1/108 (0%)
Frame = +3
Query: 12 KAEREKMKREHLNELFLDLANALDLSEP-NNGKASILIEASRLLKDLLCQIQSLKKENVS 70
KA REK++R+ LN+ F++L + L+ P KA+ILI+A R++ L + Q LK N
Sbjct: 489 KACREKLRRDRLNDKFIELGSILEPGRPAKTDKAAILIDAVRMVTQLRGEAQKLKDANSG 668
Query: 71 LLSESHYVTMEKNELKEENSSLETQIEKLQGEIQARIAQSKPDLNAPP 118
L + + +EKNEL++E L+ + EKL+ ++++ +NAPP
Sbjct: 669 LQEKIKELKVEKNELRDEKQRLKAEKEKLEQQLKS--------MNAPP 788
>TC89847 similar to GP|20127030|gb|AAM10939.1 putative bHLH transcription
factor {Arabidopsis thaliana}, partial (42%)
Length = 1030
Score = 67.8 bits (164), Expect = 3e-12
Identities = 41/108 (37%), Positives = 60/108 (54%), Gaps = 1/108 (0%)
Frame = +3
Query: 12 KAEREKMKREHLNELFLDLANALDLSEP-NNGKASILIEASRLLKDLLCQIQSLKKENVS 70
KA REK++RE LNE F DL+ L+ P K +IL +A R+L L + Q LK+ N
Sbjct: 306 KACREKLRREKLNERFCDLSAVLEPGRPVRTDKPAILDDAIRVLSQLKTEAQELKESNEK 485
Query: 71 LLSESHYVTMEKNELKEENSSLETQIEKLQGEIQARIAQSKPDLNAPP 118
LL E + EKNEL+EE L+ EK++ ++++ + PP
Sbjct: 486 LLEEIKCLKAEKNELREEKLVLKADKEKIEKQLKSMPVSPAGFMPPPP 629
>TC86369 similar to GP|15451010|gb|AAK96776.1 Unknown protein {Arabidopsis
thaliana}, partial (74%)
Length = 1500
Score = 67.0 bits (162), Expect = 5e-12
Identities = 39/108 (36%), Positives = 65/108 (60%), Gaps = 1/108 (0%)
Frame = +1
Query: 12 KAEREKMKREHLNELFLDLANALDLSEP-NNGKASILIEASRLLKDLLCQIQSLKKENVS 70
KA REK++R+ LN+ F++L + L+ P KA+ILI+A R++ L + Q LK N
Sbjct: 700 KACREKLRRDRLNDKFIELGSILEPGGPAKTDKAAILIDAVRMVTQLRGEAQKLKDANSG 879
Query: 71 LLSESHYVTMEKNELKEENSSLETQIEKLQGEIQARIAQSKPDLNAPP 118
L + + +EKNEL++E L+ + EKL+ ++++ +NAPP
Sbjct: 880 LQEKIKELKVEKNELRDEKQRLKAEKEKLEQQLKS--------MNAPP 999
>TC86368 similar to GP|15451010|gb|AAK96776.1 Unknown protein {Arabidopsis
thaliana}, partial (71%)
Length = 1071
Score = 65.9 bits (159), Expect = 1e-11
Identities = 36/99 (36%), Positives = 61/99 (61%), Gaps = 1/99 (1%)
Frame = +2
Query: 12 KAEREKMKREHLNELFLDLANALDLSEP-NNGKASILIEASRLLKDLLCQIQSLKKENVS 70
KA REK++R+ LN+ F++L + L+ P KA+ILI+A R++ L + Q LK N
Sbjct: 278 KACREKLRRDRLNDKFVELGSILEPGRPPKTDKAAILIDAVRMVTQLRGEAQKLKDSNSG 457
Query: 71 LLSESHYVTMEKNELKEENSSLETQIEKLQGEIQARIAQ 109
L + + +EKNEL++E L+ + EKL+ ++++ Q
Sbjct: 458 LQEKIKELKVEKNELRDEKQRLKAEKEKLEQQVKSMNTQ 574
>TC83223 similar to GP|8777436|dbj|BAA97026.1
gb|AAC78547.1~gene_id:MHM17.7~similar to unknown protein
{Arabidopsis thaliana}, partial (13%)
Length = 514
Score = 41.2 bits (95), Expect = 3e-04
Identities = 26/66 (39%), Positives = 35/66 (52%)
Frame = +1
Query: 9 RIHKAEREKMKREHLNELFLDLANALDLSEPNNGKASILIEASRLLKDLLCQIQSLKKEN 68
++H E+ +RE LNE F L AL KASILI A L+ L+ +I L K N
Sbjct: 193 QLHHMISERRRREKLNENFQAL-RALLPQGTKKDKASILITAKETLRSLMAEIDKLSKRN 369
Query: 69 VSLLSE 74
L+S+
Sbjct: 370 QELMSQ 387
>BQ143793 weakly similar to GP|6523547|emb| hydroxyproline-rich glycoprotein
DZ-HRGP {Volvox carteri f. nagariensis}, partial (19%)
Length = 1100
Score = 37.0 bits (84), Expect = 0.005
Identities = 20/56 (35%), Positives = 29/56 (51%), Gaps = 3/56 (5%)
Frame = +2
Query: 138 PTVDPTLQQGPTVLVVPFRPDLQAAYPAPTEITPNPPLVI---SKPHARYPTPADS 190
P++ PTL P + PF L+ YP P P+PPL++ PH +P+P S
Sbjct: 119 PSLSPTLSYPPHLFPFPF--SLKIIYPPPPLPIPSPPLLLPPPPPPHFLFPSPLPS 280
Score = 27.3 bits (59), Expect = 4.2
Identities = 16/53 (30%), Positives = 24/53 (45%)
Frame = +2
Query: 148 PTVLVVPFRPDLQAAYPAPTEITPNPPLVISKPHARYPTPADSWPSQLLGEQP 200
P L P+ P + +P P+ +P L P R+P + S+PS L P
Sbjct: 341 PPPLPPPYTPLHISHFPLPSSSPSSPFLSPPFPSLRFPLSSFSFPSSFLSLVP 499
>TC77200 similar to GP|22597162|gb|AAN03468.1 bZIP transcription factor ATB2
{Glycine max}, partial (84%)
Length = 1481
Score = 35.0 bits (79), Expect = 0.020
Identities = 16/52 (30%), Positives = 30/52 (56%)
Frame = +1
Query: 54 LKDLLCQIQSLKKENVSLLSESHYVTMEKNELKEENSSLETQIEKLQGEIQA 105
L DL+ Q+ L+KEN +L+ + T + ++ ENS L Q+ +L +++
Sbjct: 688 LDDLVSQVSKLRKENQEILTSVNITTQKYLSVEAENSVLRAQMGELSNRLES 843
>AW981132 similar to GP|4457221|gb|A putative bZIP DNA-binding protein
{Capsicum chinense}, partial (47%)
Length = 587
Score = 34.3 bits (77), Expect = 0.034
Identities = 18/56 (32%), Positives = 29/56 (51%)
Frame = +2
Query: 56 DLLCQIQSLKKENVSLLSESHYVTMEKNELKEENSSLETQIEKLQGEIQARIAQSK 111
D++ Q+ L K+N +L+ + T ++ ENS L QI +L Q+ QSK
Sbjct: 344 DMMSQMSQLTKDNSEILNSINITTQHYLNVEAENSILRAQIGELSQRFQSLTHQSK 511
>TC91130 homologue to GP|11245494|gb|AAG33640.1 SPATULA {Arabidopsis
thaliana}, partial (20%)
Length = 766
Score = 32.7 bits (73), Expect = 0.099
Identities = 27/84 (32%), Positives = 37/84 (43%), Gaps = 8/84 (9%)
Frame = +2
Query: 16 EKMKREHLNELFLDLANALDLSEPNNGKASILIEASRLLKDLLCQIQSLKKENVSLLSES 75
EK +R +NE L N + S + KAS+L EA LK L Q+Q L N L
Sbjct: 56 EKRRRSRINEKMKALQNLIPNSNKTD-KASMLDEAIEYLKQLQLQVQMLSLRNGLSLHPM 232
Query: 76 HY--------VTMEKNELKEENSS 91
++ ++ EL E N S
Sbjct: 233 YFHEGLHSLPLSRMSTELSEGNGS 304
>TC85773 weakly similar to GP|9650828|emb|CAC00658.1 common plant regulatory
factor 7 {Petroselinum crispum}, partial (62%)
Length = 945
Score = 32.7 bits (73), Expect = 0.099
Identities = 16/52 (30%), Positives = 29/52 (55%)
Frame = +1
Query: 54 LKDLLCQIQSLKKENVSLLSESHYVTMEKNELKEENSSLETQIEKLQGEIQA 105
++DL QI+ LKKEN+ + + T ++ EN+ L Q+ +L +Q+
Sbjct: 337 MEDLSNQIEQLKKENIQISTNVGVTTQMYLNVESENAILRVQMAELSHRLQS 492
>TC85772 weakly similar to GP|9650828|emb|CAC00658.1 common plant regulatory
factor 7 {Petroselinum crispum}, partial (64%)
Length = 1224
Score = 32.7 bits (73), Expect = 0.099
Identities = 16/52 (30%), Positives = 29/52 (55%)
Frame = +1
Query: 54 LKDLLCQIQSLKKENVSLLSESHYVTMEKNELKEENSSLETQIEKLQGEIQA 105
++DL QI+ LKKEN+ + + T ++ EN+ L Q+ +L +Q+
Sbjct: 826 MEDLSNQIEQLKKENIQISTNVGVTTQMYLNVESENAILRVQMAELSHRLQS 981
>BG453035 weakly similar to GP|10334495|emb putative extracellular dermal
glycoprotein {Cicer arietinum}, partial (11%)
Length = 649
Score = 30.8 bits (68), Expect = 0.38
Identities = 15/60 (25%), Positives = 30/60 (50%)
Frame = -3
Query: 137 LPTVDPTLQQGPTVLVVPFRPDLQAAYPAPTEITPNPPLVISKPHARYPTPADSWPSQLL 196
L +V P ++ G + ++ F + ++ +P +I L++ PH + P WP QL+
Sbjct: 410 LESVQPVIKSG--IWILDFGMNKKSLPVSPEKIASTNGLIMLAPHVLFEQPGLKWPLQLM 237
>BF646001 homologue to GP|20303561|gb| putative kinase {Oryza sativa
(japonica cultivar-group)}, partial (6%)
Length = 667
Score = 30.4 bits (67), Expect = 0.49
Identities = 24/98 (24%), Positives = 53/98 (53%), Gaps = 6/98 (6%)
Frame = +2
Query: 12 KAEREKMKREHL--NELFLDLANALD-LSEPNNGKASILIEASRLLKDLL---CQIQSLK 65
+ ER +++ E++ NE + ++ L + ++ A + EA+RL ++++ +I+ L+
Sbjct: 164 ECERGELETENIKNNEEIEKMKTEIEKLRKNSDSTAELEKEAARLRREVVESKVEIEKLR 343
Query: 66 KENVSLLSESHYVTMEKNELKEENSSLETQIEKLQGEI 103
K ++ V E ELK+EN +E ++ +L+ I
Sbjct: 344 KIIDEKENKIEIVEKEGKELKQENVEMEMKVRELERRI 457
>AJ501172 similar to GP|20466324|gb| unknown protein {Arabidopsis thaliana},
partial (21%)
Length = 653
Score = 30.0 bits (66), Expect = 0.64
Identities = 23/64 (35%), Positives = 36/64 (55%), Gaps = 4/64 (6%)
Frame = +3
Query: 50 ASRLLKDLLCQIQSLKKENVSL-LSESHYVTMEKNELKEENSSLETQ---IEKLQGEIQA 105
A + + D L I LK+ + L S VT+ E++E+ S ++T I+KLQGE+ A
Sbjct: 456 ADQAVVDELRAISELKRRFLKKELDLSPQVTIMLAEIQEQQSIMKTYEITIKKLQGEVDA 635
Query: 106 RIAQ 109
R +Q
Sbjct: 636 RDSQ 647
>TC92109 similar to GP|19683031|gb|AAL92653.1 HYPOTHETICAL 31.4 KDA PROTEIN
{Dictyostelium discoideum}, partial (2%)
Length = 653
Score = 30.0 bits (66), Expect = 0.64
Identities = 21/64 (32%), Positives = 31/64 (47%)
Frame = +2
Query: 120 LELESPEQTTFSGQSFQLPTVDPTLQQGPTVLVVPFRPDLQAAYPAPTEITPNPPLVISK 179
++L+ EQ F+ + Q+ L+Q PT +P P+P+ TP PP IS
Sbjct: 119 MDLQDLEQLGFADDAQQVLAAISILKQDPTGTTKEVKP------PSPSPPTPPPPTSIS- 277
Query: 180 PHAR 183
HAR
Sbjct: 278 DHAR 289
>AW586676 similar to GP|806720|gb|AA arabinogalactan-protein precursor
{Nicotiana alata}, partial (22%)
Length = 590
Score = 29.6 bits (65), Expect = 0.84
Identities = 17/56 (30%), Positives = 26/56 (46%), Gaps = 1/56 (1%)
Frame = +2
Query: 136 QLPTVDPTLQQGPTVLVVPFRPDLQAAYPAPTEITPNP-PLVISKPHARYPTPADS 190
Q P+ PT+ T + P A+ P P + P+P P I+ P + P +DS
Sbjct: 86 QSPSSSPTISPVATPAISPSADSPAASAPIPVKNAPSPSPSAINSPPSPPPASSDS 253
>TC89647 similar to GP|20258923|gb|AAM14177.1 unknown protein {Arabidopsis
thaliana}, partial (83%)
Length = 981
Score = 29.3 bits (64), Expect = 1.1
Identities = 21/92 (22%), Positives = 41/92 (43%), Gaps = 8/92 (8%)
Frame = +1
Query: 65 KKENVSLLSESHYVTMEKNELKEENSSLET--QIEKLQGEIQARIAQSKPD------LNA 116
K E + + + T+ + ++ +E L+ +++ + +Q ++ + P
Sbjct: 493 KLEKIGYVDSENETTIPEPKVAKETIDLKEIKRVDHILASLQRKLPPAPPPPPFPEGYVP 672
Query: 117 PPQLELESPEQTTFSGQSFQLPTVDPTLQQGP 148
P E E +T + +S PTVDP L QGP
Sbjct: 673 PSTAETEKGPETQEAAESLA-PTVDPILDQGP 765
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.309 0.128 0.355
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 5,465,191
Number of Sequences: 36976
Number of extensions: 74498
Number of successful extensions: 585
Number of sequences better than 10.0: 77
Number of HSP's better than 10.0 without gapping: 533
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 577
length of query: 203
length of database: 9,014,727
effective HSP length: 91
effective length of query: 112
effective length of database: 5,649,911
effective search space: 632790032
effective search space used: 632790032
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.7 bits)
S2: 56 (26.2 bits)
Medicago: description of AC146548.2