Medicago
BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= AC146527.10 - phase: 0 /pseudo
         (129 letters)

Database: MTGI 
           36,976 sequences; 27,044,181 total letters

Searching..................................................done


                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

BG585866                                                              123  1e-33
TC81772                                                                40  3e-04
AJ497561 weakly similar to GP|10140689|gb putative non-LTR retro...    36  0.005
AW688528                                                               31  0.15
TC86469 similar to GP|21464654|emb|CAD30328. acid phosphatase {L...    29  0.58
TC81691 weakly similar to GP|6016717|gb|AAF01543.1| unknown prot...    28  0.99
TC91177 similar to GP|9294656|dbj|BAB03005.1 gb|AAF30317.1~gene_...    28  0.99
TC93885 weakly similar to GP|18204817|gb|AAH21490.1 Similar to d...    28  1.3
AL367200 weakly similar to GP|9955528|emb putative protein {Arab...    28  1.3
BQ751661 weakly similar to PIR|T39572|T39 probable proteinase su...    27  1.7
TC80054 SP|Q28009|FUS_BOVIN RNA-binding protein FUS (Pigpen prot...    27  2.2
TC78016 similar to GP|21537191|gb|AAM61532.1 PRL1 protein {Arabi...    27  2.2
BE239650 similar to PIR|D87615|D876 conserved hypothetical prote...    27  2.9
BQ122396 similar to GP|7939524|dbj| contains similarity to unkno...    26  3.8
BQ137350 weakly similar to GP|11385579|gb| In2-1 protein {Glycin...    26  3.8
BE239441 weakly similar to PIR|T49216|T492 hypothetical protein ...    26  3.8
TC90474                                                                26  3.8
BQ140218 homologue to GP|23495108|gb hypothetical protein {Plasm...    26  3.8
AW775796 similar to PIR|T08972|T08 hypothetical protein F19B15.2...    26  3.8
BQ137255 homologue to PIR|B34768|B347 ORF5 protein - Orf virus (...    26  3.8

>BG585866 
          Length = 828

 Score =  123 bits (308), Expect(2) = 1e-33
 Identities = 54/90 (60%), Positives = 69/90 (76%)
 Frame = +3

Query: 39  IIRAKNILKEGYTWRIGSGSSSFWFSNWNSLGPIGSYVPIIDIHDLHLSVRDVLSAPGNH 98
           IIR KN+LK GYTWR GSG+SSFW++NW+SLG +G+  P +DIHDLHL+V+DV +  G H
Sbjct: 84  IIRDKNVLKSGYTWRAGSGNSSFWYTNWSSLGLLGTQAPFVDIHDLHLTVKDVFTTGGQH 263

Query: 99  TQVLYTNLPSAVENHINNIHIQFNDNIEDA 128
           TQ LYT LP+ +   INN H+ FN +I DA
Sbjct: 264 TQSLYTILPTDIAEVINNTHLNFNASIGDA 353



 Score = 35.4 bits (80), Expect(2) = 1e-33
 Identities = 17/28 (60%), Positives = 21/28 (74%), Gaps = 1/28 (3%)
 Frame = +1

Query: 13 LSNKYTGGHNTL-LASASSNSSPTWSSI 39
          L N Y+ GHN L +A+A  NSSP+WSSI
Sbjct: 1  LFNNYSSGHNFLHIAAAHRNSSPSWSSI 84


>TC81772 
          Length = 982

 Score = 40.0 bits (92), Expect = 3e-04
 Identities = 19/50 (38%), Positives = 29/50 (58%), Gaps = 1/50 (2%)
 Frame = -2

Query: 31  NSSPTWSSIIRAKNILKEGYTWRIGSGSS-SFWFSNWNSLGPIGSYVPII 79
           N S  W S+   + +L+EG+ W+IG GSS + W  +W S    G Y+P +
Sbjct: 708 NPSYIWCSV*TLRMVLEEGHHWKIGDGSSINIWTDHWLS----GMYIPCV 571


>AJ497561 weakly similar to GP|10140689|gb putative non-LTR retroelement
           reverse transcriptase {Oryza sativa (japonica
           cultivar-group)}, partial (2%)
          Length = 621

 Score = 35.8 bits (81), Expect = 0.005
 Identities = 22/72 (30%), Positives = 33/72 (45%), Gaps = 5/72 (6%)
 Frame = +2

Query: 5   TNKLWVSLLSNKYTGGHNTLLASASSNSSPTWSSIIRAKNILKEGYTWRIGSG-----SS 59
           +  L   +L  KY    +   A+   N S TW S++  +++L  G+ W IG G     SS
Sbjct: 404 STSLLSKILKFKYFPQWDFSYANLGHNPSFTWRSLLSTQSLLTLGHRWMIGDGSQINVSS 583

Query: 60  SFWFSNWNSLGP 71
             W  N  +L P
Sbjct: 584 MSWIRNRPTLRP 619


>AW688528 
          Length = 631

 Score = 30.8 bits (68), Expect = 0.15
 Identities = 19/56 (33%), Positives = 27/56 (47%), Gaps = 1/56 (1%)
 Frame = +3

Query: 74  SYVPIIDIHDLHLSVRDVL-SAPGNHTQVLYTNLPSAVENHINNIHIQFNDNIEDA 128
           S +PI+     H +  D + S+ G+  +        A ENHI  +  QFN  IEDA
Sbjct: 198 SIIPILHHQIPHSTYSDQMDSSSGDRPKAAVNRANVAGENHIPRLKNQFNSKIEDA 365


>TC86469 similar to GP|21464654|emb|CAD30328. acid phosphatase {Lupinus
           luteus}, partial (92%)
          Length = 1737

 Score = 28.9 bits (63), Expect = 0.58
 Identities = 14/28 (50%), Positives = 17/28 (60%), Gaps = 2/28 (7%)
 Frame = +1

Query: 50  YTWRIGSGSSS--FWFSNWNSLGPIGSY 75
           Y +RIGSG SS  FWF     +GP  +Y
Sbjct: 436 YYYRIGSGDSSREFWFETPPKVGPDATY 519


>TC81691 weakly similar to GP|6016717|gb|AAF01543.1| unknown protein
           {Arabidopsis thaliana}, complete
          Length = 1465

 Score = 28.1 bits (61), Expect = 0.99
 Identities = 15/32 (46%), Positives = 18/32 (55%), Gaps = 1/32 (3%)
 Frame = +2

Query: 52  WRIGSGSSSFWFS-NWNSLGPIGSYVPIIDIH 82
           W IGS SSS WFS N N    I   + I+ +H
Sbjct: 935 WTIGSSSSSLWFSVNCNRWS*INKDLYILCVH 1030


>TC91177 similar to GP|9294656|dbj|BAB03005.1
           gb|AAF30317.1~gene_id:F14O13.6~similar to unknown
           protein {Arabidopsis thaliana}, partial (9%)
          Length = 1298

 Score = 28.1 bits (61), Expect = 0.99
 Identities = 13/36 (36%), Positives = 19/36 (52%)
 Frame = -1

Query: 87  SVRDVLSAPGNHTQVLYTNLPSAVENHINNIHIQFN 122
           SV   L +P NH    +T      +NH NN+H+ +N
Sbjct: 545 SVHRTLKSPSNHLLTRHT------QNHRNNLHLDWN 456


>TC93885 weakly similar to GP|18204817|gb|AAH21490.1 Similar to
           death-associated protein kinase 1 {Mus musculus},
           partial (2%)
          Length = 827

 Score = 27.7 bits (60), Expect = 1.3
 Identities = 13/41 (31%), Positives = 23/41 (55%)
 Frame = -2

Query: 82  HDLHLSVRDVLSAPGNHTQVLYTNLPSAVENHINNIHIQFN 122
           HD+ + V  + + P N    ++   P   +NHIN I++QF+
Sbjct: 601 HDIFVIVTAI*NPPANS---IFRV*PKTSDNHINRIYLQFS 488


>AL367200 weakly similar to GP|9955528|emb putative protein {Arabidopsis
           thaliana}, partial (9%)
          Length = 366

 Score = 27.7 bits (60), Expect = 1.3
 Identities = 15/28 (53%), Positives = 17/28 (60%)
 Frame = +3

Query: 41  RAKNILKEGYTWRIGSGSSSFWFSNWNS 68
           R  N+    Y W  GSGSSS+ FS WNS
Sbjct: 150 RGDNV*LVLYVW--GSGSSSYDFS*WNS 227


>BQ751661 weakly similar to PIR|T39572|T39 probable proteinase subunit -
           fission yeast (Schizosaccharomyces pombe), partial (22%)
          Length = 708

 Score = 27.3 bits (59), Expect = 1.7
 Identities = 14/38 (36%), Positives = 24/38 (62%)
 Frame = -1

Query: 29  SSNSSPTWSSIIRAKNILKEGYTWRIGSGSSSFWFSNW 66
           SS+SS + +  I +  ++ E Y WR GS SSS + +++
Sbjct: 285 SSSSSLSLAR*ICSPRLISERYFWRRGSSSSSTFLASF 172


>TC80054 SP|Q28009|FUS_BOVIN RNA-binding protein FUS (Pigpen protein).
           [Bovine] {Bos taurus}, partial (4%)
          Length = 988

 Score = 26.9 bits (58), Expect = 2.2
 Identities = 14/41 (34%), Positives = 21/41 (51%), Gaps = 4/41 (9%)
 Frame = +2

Query: 84  LHLSVRDVLSAPGNHTQVLYTNLPSAVEN----HINNIHIQ 120
           LHL +  +L+   NH  ++ TN    +      HINN+H Q
Sbjct: 86  LHLQLMGLLTITANHLPLVITNRDRDILRMAMVHINNLHNQ 208


>TC78016 similar to GP|21537191|gb|AAM61532.1 PRL1 protein {Arabidopsis
            thaliana}, partial (81%)
          Length = 1759

 Score = 26.9 bits (58), Expect = 2.2
 Identities = 16/74 (21%), Positives = 29/74 (38%), Gaps = 7/74 (9%)
 Frame = +1

Query: 2    VQSTNKLWVSLLSNKYTGGHNTLLASASSNSSPTWSSIIRAKNIL-------KEGYTWRI 54
            ++S  + W+S+L +K    H  ++  +S      + +I      L            W  
Sbjct: 1090 IKSLFEQWLSILKSKLLHLHQLIILKSSPFQKENFVTICSLNRKLLSMQWLSMRRVLWLP 1269

Query: 55   GSGSSSFWFSNWNS 68
            G  + S WF +W S
Sbjct: 1270 GGDNGSMWFWDWKS 1311


>BE239650 similar to PIR|D87615|D876 conserved hypothetical protein CC2958
           [imported] - Caulobacter crescentus, partial (10%)
          Length = 628

 Score = 26.6 bits (57), Expect = 2.9
 Identities = 19/61 (31%), Positives = 30/61 (49%), Gaps = 4/61 (6%)
 Frame = +2

Query: 11  SLLSNKYTGGHN----TLLASASSNSSPTWSSIIRAKNILKEGYTWRIGSGSSSFWFSNW 66
           +L  N+Y+   N     LL   S +S  + + I   +NI    Y W  G+ +S FW+ NW
Sbjct: 305 NLAFNRYSYCRN*F*LKLLIQISYSSWKSLTII*GRRNI----YYWFRGNNTSFFWWYNW 472

Query: 67  N 67
           +
Sbjct: 473 S 475


>BQ122396 similar to GP|7939524|dbj| contains similarity to unknown
          protein~gb|AAD25764.1~gene_id:K14B15.5 {Arabidopsis
          thaliana}, partial (7%)
          Length = 498

 Score = 26.2 bits (56), Expect = 3.8
 Identities = 11/20 (55%), Positives = 12/20 (60%), Gaps = 1/20 (5%)
 Frame = +1

Query: 52 WRIGSGSS-SFWFSNWNSLG 70
          WRI   S+  FWFS WN  G
Sbjct: 61 WRISFSSA*GFWFSLWNKSG 120


>BQ137350 weakly similar to GP|11385579|gb| In2-1 protein {Glycine max},
           partial (34%)
          Length = 897

 Score = 26.2 bits (56), Expect = 3.8
 Identities = 14/45 (31%), Positives = 23/45 (51%)
 Frame = +1

Query: 84  LHLSVRDVLSAPGNHTQVLYTNLPSAVENHINNIHIQFNDNIEDA 128
           +H   R V      H +  YT++   + N INN +I  N+ I+D+
Sbjct: 763 MHNH*RRVRCLQSTHNE--YTDIDDRITNIINNRYIDTNNEIDDS 891


>BE239441 weakly similar to PIR|T49216|T492 hypothetical protein F27K19.220 -
           Arabidopsis thaliana, partial (4%)
          Length = 451

 Score = 26.2 bits (56), Expect = 3.8
 Identities = 13/46 (28%), Positives = 19/46 (41%)
 Frame = -1

Query: 79  IDIHDLHLSVRDVLSAPGNHTQVLYTNLPSAVENHINNIHIQFNDN 124
           + IHD  +SVR        H   + +  P    +H+ N H   N N
Sbjct: 151 LQIHDFPVSVRQHQ*LQIQHFHAV*SAAPQTFSHHV*NTHFHHNSN 14


>TC90474 
          Length = 744

 Score = 26.2 bits (56), Expect = 3.8
 Identities = 12/39 (30%), Positives = 20/39 (50%), Gaps = 4/39 (10%)
 Frame = +2

Query: 36 WSSIIRAKNILKEG----YTWRIGSGSSSFWFSNWNSLG 70
          WS +++      +G    +TWR+G G+S      W+ LG
Sbjct: 44 WSDLVKVIAC**QGCVMEFTWRVGEGASPTQGVEWSRLG 160


>BQ140218 homologue to GP|23495108|gb hypothetical protein {Plasmodium
           falciparum 3D7}, partial (1%)
          Length = 211

 Score = 26.2 bits (56), Expect = 3.8
 Identities = 9/21 (42%), Positives = 13/21 (61%)
 Frame = +2

Query: 50  YTWRIGSGSSSFWFSNWNSLG 70
           +TWR+G G+S      W+ LG
Sbjct: 140 FTWRVGKGASPTQGVEWSKLG 202


>AW775796 similar to PIR|T08972|T08 hypothetical protein F19B15.200 -
           Arabidopsis thaliana, partial (96%)
          Length = 714

 Score = 26.2 bits (56), Expect = 3.8
 Identities = 10/39 (25%), Positives = 22/39 (55%)
 Frame = +1

Query: 90  DVLSAPGNHTQVLYTNLPSAVENHINNIHIQFNDNIEDA 128
           D++S     T V + +LPS   N + N+H + +  ++++
Sbjct: 202 DLVSKDKIGTSVYFWSLPSCAGNQLRNVHQKLDSELQNS 318


>BQ137255 homologue to PIR|B34768|B347 ORF5 protein - Orf virus (strain NZ2),
           partial (10%)
          Length = 1161

 Score = 26.2 bits (56), Expect = 3.8
 Identities = 10/30 (33%), Positives = 17/30 (56%)
 Frame = -3

Query: 41  RAKNILKEGYTWRIGSGSSSFWFSNWNSLG 70
           R +   +  + WR+ SG+ SF+F +  S G
Sbjct: 175 RFRRAARRAWRWRVVSGARSFFFLSGGSFG 86


  Database: MTGI
    Posted date:  Oct 22, 2004  3:39 PM
  Number of letters in database: 27,044,181
  Number of sequences in database:  36,976
  
Lambda     K      H
   0.314    0.130    0.398 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 5,173,558
Number of Sequences: 36976
Number of extensions: 79513
Number of successful extensions: 426
Number of sequences better than 10.0: 52
Number of HSP's better than 10.0 without gapping: 421
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 425
length of query: 129
length of database: 9,014,727
effective HSP length: 85
effective length of query: 44
effective length of database: 5,871,767
effective search space: 258357748
effective search space used: 258357748
frameshift window, decay const: 50,  0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (22.0 bits)
S2: 52 (24.6 bits)


Medicago: description of AC146527.10