BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146527.1 - phase: 0
(468 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC88598 similar to GP|14532590|gb|AAK64023.1 unknown protein {Ar... 395 e-110
TC90502 similar to PIR|G86475|G86475 unknown protein 13877-1716... 260 9e-70
TC90109 weakly similar to GP|22202689|dbj|BAC07347. contains EST... 70 2e-12
TC79807 similar to GP|2443886|gb|AAB71479.1| Unknown protein {Ar... 66 2e-11
BF650858 similar to PIR|G96552|G96 unknown protein 66348-64527 ... 40 0.001
TC79684 similar to GP|9758131|dbj|BAB08623.1 gb|AAB71479.1|~gene... 39 0.003
TC80543 similar to PIR|T47646|T47646 hypothetical protein T15C9.... 33 0.31
BM779886 weakly similar to GP|22324479|db putative ligand-gated ... 31 1.2
TC86691 similar to GP|15810175|gb|AAL06989.1 At2g03890/T18C20.9 ... 29 3.4
TC78586 similar to PIR|T01041|T01041 hypothetical protein YUP8H1... 29 4.4
BE124312 similar to PIR|H96732|H96 hypothetical protein F15H11.7... 28 5.8
AW559941 28 9.8
TC79980 weakly similar to GP|15787901|gb|AAL07542.1 resistance g... 28 9.8
>TC88598 similar to GP|14532590|gb|AAK64023.1 unknown protein {Arabidopsis
thaliana}, partial (39%)
Length = 921
Score = 395 bits (1015), Expect = e-110
Identities = 200/200 (100%), Positives = 200/200 (100%)
Frame = +2
Query: 269 FDEASRSTIPPINIVQENGIDDQETTYALNHRKTLRQLIRDGEIDAAFGKLREWYPQIAE 328
FDEASRSTIPPINIVQENGIDDQETTYALNHRKTLRQLIRDGEIDAAFGKLREWYPQIAE
Sbjct: 2 FDEASRSTIPPINIVQENGIDDQETTYALNHRKTLRQLIRDGEIDAAFGKLREWYPQIAE 181
Query: 329 DNTSAMCFLLHCQKFIELVRVGALEEAVKYGRIELSSFFGLSLFEDIVQDCVALLAYERP 388
DNTSAMCFLLHCQKFIELVRVGALEEAVKYGRIELSSFFGLSLFEDIVQDCVALLAYERP
Sbjct: 182 DNTSAMCFLLHCQKFIELVRVGALEEAVKYGRIELSSFFGLSLFEDIVQDCVALLAYERP 361
Query: 389 LESAVGYLLKDSQREVVADAVNAMILSTNPNIKVTKNCLHSNLERLLRQLTACCLERRSL 448
LESAVGYLLKDSQREVVADAVNAMILSTNPNIKVTKNCLHSNLERLLRQLTACCLERRSL
Sbjct: 362 LESAVGYLLKDSQREVVADAVNAMILSTNPNIKVTKNCLHSNLERLLRQLTACCLERRSL 541
Query: 449 SGEQGEAFQLQRVLSSGKRS 468
SGEQGEAFQLQRVLSSGKRS
Sbjct: 542 SGEQGEAFQLQRVLSSGKRS 601
>TC90502 similar to PIR|G86475|G86475 unknown protein 13877-17163
[imported] - Arabidopsis thaliana, partial (21%)
Length = 567
Score = 260 bits (664), Expect = 9e-70
Identities = 135/159 (84%), Positives = 138/159 (85%), Gaps = 2/159 (1%)
Frame = +3
Query: 1 MKETNGKNTIEKPDQDQNQDLGFHFLELSRRKAKLDSEDFEVDDEEKEESPSELNTINSS 60
MKETNGKNTIEKPDQDQNQDLGFHFLELSRRKAKLDSEDFEVDDEEKEESPSELNTINSS
Sbjct: 81 MKETNGKNTIEKPDQDQNQDLGFHFLELSRRKAKLDSEDFEVDDEEKEESPSELNTINSS 260
Query: 61 GGFVVVATDKLSVKYTSVNLHGHDVGVIQANKVAPMKRIVYYFEIFVKDAGVKGQVSIGF 120
GGFVVVATDKLSVKYTSVNLHGHDVGVIQANKVAPMKRIVYYFEIFVKDAGVKGQVS
Sbjct: 261 GGFVVVATDKLSVKYTSVNLHGHDVGVIQANKVAPMKRIVYYFEIFVKDAGVKGQVSDWV 440
Query: 121 TTESF-KMRRQPGWEANSCGYHGDDGFLY-RGQGKGEAF 157
F WEAN+CGYHGDDGF + + KGE F
Sbjct: 441 YHREF*DEETNLVWEANNCGYHGDDGFSFIVDRVKGECF 557
Score = 39.3 bits (90), Expect = 0.003
Identities = 29/57 (50%), Positives = 31/57 (53%), Gaps = 7/57 (12%)
Frame = +1
Query: 111 GVKGQVSIGFTTESFKMRRQPGWEANSCG------YHGDDGFLYRGQG-KGEAFGPT 160
G+KG+ IGFTTESFKMRR P W CG G F G KG AFGPT
Sbjct: 412 GLKGRFPIGFTTESFKMRR-PTW----CGRLITVDIMGMMVFPLSWTGLKGNAFGPT 567
>TC90109 weakly similar to GP|22202689|dbj|BAC07347. contains EST
D48148(S14217)~similar to Arabidopsis thaliana
chromosome 1 F6F9.23~unknown protein, partial (20%)
Length = 1179
Score = 69.7 bits (169), Expect = 2e-12
Identities = 69/242 (28%), Positives = 113/242 (46%), Gaps = 2/242 (0%)
Frame = +2
Query: 215 FGQKPFTFDLKEYEAQERMKQQIKIEEVPVSPNASYGIVRSYLLHYGYEDTLNSFDEASR 274
FG+ F + R I I E VS IV SYL+H YE++ SF +
Sbjct: 74 FGESQF-HAYSTMDVDPRQYDNIAISEKDVS-----NIVLSYLIHNCYEESAESFMAGTG 235
Query: 275 STIPPINIVQENGIDDQETTYALNHRKTLRQLIRDGEIDAAFGKLREWYPQIAEDNTSAM 334
+ P + +D+ E RK + + +G A + P+I NT +
Sbjct: 236 AKRPT------DYLDNMEK------RKKIFHSVLEGNALKAIELTEQLTPEILVKNTDLL 379
Query: 335 CFLLHCQKFIELVRVGALEEAVKYGRIELSSFFGLSLFEDIVQDCVALLAYERPLESAVG 394
LL F+ELVR EA+ + + +LS F + + +QD +ALLAY+ P +S +
Sbjct: 380 FDLLSLH-FVELVRSRKCTEALDFAQTKLSPFGKDGQYTEKLQDFMALLAYKEPEDSPMF 556
Query: 395 YLLKDSQREVVADAVNAMILS--TNPNIKVTKNCLHSNLERLLRQLTACCLERRSLSGEQ 452
+LL R+ +AD++N IL+ PN ++ +E+L++Q T + R+ L+ E
Sbjct: 557 HLLSLEYRQELADSLNRTILAYFNLPN--------YTAMEKLIQQTT---VVRQCLNEEA 703
Query: 453 GE 454
G+
Sbjct: 704 GK 709
>TC79807 similar to GP|2443886|gb|AAB71479.1| Unknown protein {Arabidopsis
thaliana}, partial (92%)
Length = 1129
Score = 66.2 bits (160), Expect = 2e-11
Identities = 55/195 (28%), Positives = 95/195 (48%), Gaps = 2/195 (1%)
Frame = +2
Query: 232 RMKQQIKIEEVPVSPNASYGIVRSYLLHYGYEDTLNSFDEASRSTIPPINIVQENGIDDQ 291
R + + K+ V + +V ++L+ G+ D F + S T P I++
Sbjct: 212 REEWEKKLNNVKIRKEDMNKLVMNFLVTEGFVDAAEKFRKES-GTEPDIDLA-------- 364
Query: 292 ETTYALNHRKTLRQLIRDGEIDAAFGKLREWYPQIAEDNTSAMCFLLHCQKFIELVRVGA 351
+ R +++ ++ G ++ A K+ + P+I + N + F L Q+ IEL+R G
Sbjct: 365 ----TITDRMAVKKAVQSGNVEDAIEKVNDLNPEILDTNPQ-LFFHLQQQRLIELIRNGK 529
Query: 352 LEEAVKYGRIELS--SFFGLSLFEDIVQDCVALLAYERPLESAVGYLLKDSQREVVADAV 409
+EEA+++ + EL+ S E++ + VALLA+E VG LL SQR A V
Sbjct: 530 IEEALEFAQEELAPRGEENQSFLEEL-ERTVALLAFEDVSNCPVGELLDISQRLKTASEV 706
Query: 410 NAMILSTNPNIKVTK 424
NA IL++ + K K
Sbjct: 707 NAAILTSQSHEKDPK 751
>BF650858 similar to PIR|G96552|G96 unknown protein 66348-64527 [imported] -
Arabidopsis thaliana, partial (28%)
Length = 536
Score = 40.4 bits (93), Expect = 0.001
Identities = 23/72 (31%), Positives = 33/72 (44%)
Frame = +1
Query: 101 YYFEIFVKDAGVKGQVSIGFTTESFKMRRQPGWEANSCGYHGDDGFLYRGQGKGEAFGPT 160
+YFEI V G G +G+T E ++ G++ NS GY DG +
Sbjct: 226 WYFEIKVVRLGESGHTRLGWTMEKGDLQAPVGYDGNSFGYRDIDGSKIHKALRENYGDEG 405
Query: 161 YTTGDIVGAGIN 172
Y GD++G IN
Sbjct: 406 YGEGDVIGFYIN 441
>TC79684 similar to GP|9758131|dbj|BAB08623.1
gb|AAB71479.1|~gene_id:MUD21.5~similar to unknown
protein {Arabidopsis thaliana}, partial (52%)
Length = 1792
Score = 39.3 bits (90), Expect = 0.003
Identities = 23/54 (42%), Positives = 31/54 (56%)
Frame = +3
Query: 301 KTLRQLIRDGEIDAAFGKLREWYPQIAEDNTSAMCFLLHCQKFIELVRVGALEE 354
+ +R+ + GEID A LR P I D+ + F LH QKFIEL+R G E+
Sbjct: 291 RQIRRSLETGEIDTAVDLLRLHAPSILNDHR--ILFRLHKQKFIELLRKGTNED 446
>TC80543 similar to PIR|T47646|T47646 hypothetical protein T15C9.70 -
Arabidopsis thaliana, partial (60%)
Length = 1017
Score = 32.7 bits (73), Expect = 0.31
Identities = 21/56 (37%), Positives = 33/56 (58%)
Frame = +1
Query: 332 SAMCFLLHCQKFIELVRVGALEEAVKYGRIELSSFFGLSLFEDIVQDCVALLAYER 387
S + F L Q+FIELVR + A+ Y + L+ + G + E +Q+ ALLA++R
Sbjct: 157 SKLEFQLRLQEFIELVRTESNLRAIAYAKKYLAPWAGNHMKE--LQEVTALLAFKR 318
>BM779886 weakly similar to GP|22324479|db putative ligand-gated channel-like
protein {Oryza sativa (japonica cultivar-group)},
partial (11%)
Length = 828
Score = 30.8 bits (68), Expect = 1.2
Identities = 23/71 (32%), Positives = 36/71 (50%)
Frame = -3
Query: 338 LHCQKFIELVRVGALEEAVKYGRIELSSFFGLSLFEDIVQDCVALLAYERPLESAVGYLL 397
+H + F ++ G + K ++ LSSF+GL L I+ +L R + VG+
Sbjct: 484 IHEKWFCKMGCPGERKRNSKPDQLHLSSFWGLYLSCGIISVVALVLFLLRMISQYVGF-- 311
Query: 398 KDSQREVVADA 408
K SQ EVVA +
Sbjct: 310 KQSQNEVVASS 278
>TC86691 similar to GP|15810175|gb|AAL06989.1 At2g03890/T18C20.9 {Arabidopsis
thaliana}, partial (76%)
Length = 2658
Score = 29.3 bits (64), Expect = 3.4
Identities = 15/44 (34%), Positives = 22/44 (49%)
Frame = +2
Query: 33 AKLDSEDFEVDDEEKEESPSELNTINSSGGFVVVATDKLSVKYT 76
+KLD E +DE EESP E T ++ + +S+K T
Sbjct: 2000 SKLDESIEEEEDENDEESPQEFGTFSAQEKVATIPEITVSLKNT 2131
>TC78586 similar to PIR|T01041|T01041 hypothetical protein YUP8H12R.24 -
Arabidopsis thaliana, partial (38%)
Length = 2831
Score = 28.9 bits (63), Expect = 4.4
Identities = 17/48 (35%), Positives = 26/48 (53%), Gaps = 7/48 (14%)
Frame = +1
Query: 11 EKPDQDQNQDLGFHFLELSRRKAKL-------DSEDFEVDDEEKEESP 51
E+ ++D N+D GF L + ++AKL + EV EE EE+P
Sbjct: 427 EETEEDDNEDKGFVKLTKAEKRAKLKKSRKEGKKQGKEVAKEEVEEAP 570
>BE124312 similar to PIR|H96732|H96 hypothetical protein F15H11.7 [imported]
- Arabidopsis thaliana, partial (33%)
Length = 629
Score = 28.5 bits (62), Expect = 5.8
Identities = 23/89 (25%), Positives = 38/89 (41%), Gaps = 4/89 (4%)
Frame = +1
Query: 55 NTINSSGGFVVVATDKLSVKYTSVNLHGHDVGVIQANK--VAPMKRIVYYFEIFV--KDA 110
N G ++VV K+ ++ + L G D G+ K P + + I ++A
Sbjct: 310 NHFLDDGAYMVV---KIIIEMVRMKLGGSDEGIGSLIKDLEEPYESVELRINIISEPRNA 480
Query: 111 GVKGQVSIGFTTESFKMRRQPGWEANSCG 139
KG ++ + RR GWE +SCG
Sbjct: 481 KAKGSEALETFRNYIEERRLKGWELDSCG 567
>AW559941
Length = 608
Score = 27.7 bits (60), Expect = 9.8
Identities = 18/50 (36%), Positives = 24/50 (48%)
Frame = +3
Query: 32 KAKLDSEDFEVDDEEKEESPSELNTINSSGGFVVVATDKLSVKYTSVNLH 81
+ DS DF D E E ELNTI S + + KLS ++ +LH
Sbjct: 294 ETNFDSFDF---DFEIERPTMELNTIFSDNSIIETISPKLSPSSSNSSLH 434
>TC79980 weakly similar to GP|15787901|gb|AAL07542.1 resistance gene analog
NBS7 {Helianthus annuus}, partial (33%)
Length = 1258
Score = 27.7 bits (60), Expect = 9.8
Identities = 25/88 (28%), Positives = 37/88 (41%), Gaps = 1/88 (1%)
Frame = +3
Query: 23 FHFLELSRRKAKLDSEDFEVDDEEKEESPSELNTINSSGGFVVVATDKLSVKY-TSVNLH 81
+H L + K + + DDEE + SPS L I S +VV LS Y TS
Sbjct: 120 YHALRNKKIKTFFPHDQIQNDDEELQLSPSILKAIQESRISIVV----LSKNYATSTRCL 287
Query: 82 GHDVGVIQANKVAPMKRIVYYFEIFVKD 109
V ++Q K+ ++E+ D
Sbjct: 288 NELVIILQCMKMKNQLVWPIFYEVHSSD 371
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.317 0.135 0.388
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 12,267,473
Number of Sequences: 36976
Number of extensions: 147677
Number of successful extensions: 748
Number of sequences better than 10.0: 26
Number of HSP's better than 10.0 without gapping: 736
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 744
length of query: 468
length of database: 9,014,727
effective HSP length: 100
effective length of query: 368
effective length of database: 5,317,127
effective search space: 1956702736
effective search space used: 1956702736
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 60 (27.7 bits)
Medicago: description of AC146527.1