BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146343.8 + phase: 0 /pseudo
(379 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BQ149019 similar to GP|21554890|gb unknown {Arabidopsis thaliana... 181 6e-61
TC77285 homologue to GP|20269901|gb|AAM18095.1 putative sodium-d... 192 2e-49
BE321838 135 3e-32
TC89740 similar to GP|16648877|gb|AAL24290.1 Unknown protein {Ar... 124 8e-29
TC92539 similar to GP|16648877|gb|AAL24290.1 Unknown protein {Ar... 118 3e-27
BF632420 weakly similar to GP|21618116|gb| unknown {Arabidopsis ... 52 3e-07
BE319834 similar to GP|21618116|gb unknown {Arabidopsis thaliana... 37 0.013
BG586394 similar to GP|7295463|gb| CG4835 gene product {Drosophi... 31 0.69
TC80570 similar to GP|7643796|gb|AAF65513.1| GTP-binding protein... 30 1.2
TC88542 similar to GP|17065530|gb|AAL32919.1 Unknown protein {Ar... 30 1.2
TC76510 homologue to GP|1616609|emb|CAA69931.1 PR10-1 protein {M... 30 2.0
TC89433 similar to GP|13277220|emb|CAC34409. ZF-HD homeobox prot... 28 4.5
TC84163 similar to GP|13365608|dbj|BAB39154. avicelase 2 {Humico... 28 4.5
TC81738 similar to GP|17065530|gb|AAL32919.1 Unknown protein {Ar... 28 5.8
BQ149911 GP|13940261|em ATPase subunit 6 {Cebus albifrons}, part... 28 7.6
TC91327 similar to GP|22597168|gb|AAN03471.1 unknown protein {Gl... 28 7.6
TC91814 weakly similar to GP|10177113|dbj|BAB10403. gb|AAD32907.... 28 7.6
TC81105 weakly similar to PIR|F84721|F84721 probable RING zinc f... 27 9.9
TC84889 similar to GP|21537003|gb|AAM61344.1 unknown {Arabidopsi... 27 9.9
BF647972 similar to GP|22532805|gb| C. elegans PQN-75 protein (c... 27 9.9
>BQ149019 similar to GP|21554890|gb unknown {Arabidopsis thaliana}, partial
(36%)
Length = 619
Score = 181 bits (460), Expect(2) = 6e-61
Identities = 93/93 (100%), Positives = 93/93 (100%)
Frame = +1
Query: 245 AVAMSKSILQVVLAPVTLGLLLNTYAKPVVSILRPVMPFVAMICTSLCIGSPLAINRSQI 304
AVAMSKSILQVVLAPVTLGLLLNTYAKPVVSILRPVMPFVAMICTSLCIGSPLAINRSQI
Sbjct: 16 AVAMSKSILQVVLAPVTLGLLLNTYAKPVVSILRPVMPFVAMICTSLCIGSPLAINRSQI 195
Query: 305 LSGEGLRLVAPVLIFHAAAFTLGYWFSNLPSLR 337
LSGEGLRLVAPVLIFHAAAFTLGYWFSNLPSLR
Sbjct: 196 LSGEGLRLVAPVLIFHAAAFTLGYWFSNLPSLR 294
Score = 70.9 bits (172), Expect(2) = 6e-61
Identities = 30/34 (88%), Positives = 33/34 (96%)
Frame = +3
Query: 346 TLSQAGRTGEQDNFIMHWNAELHTCRPSSNTIFG 379
++ +AGRTGEQDNFIMHWNAELHTCRPSSNTIFG
Sbjct: 282 SILKAGRTGEQDNFIMHWNAELHTCRPSSNTIFG 383
>TC77285 homologue to GP|20269901|gb|AAM18095.1 putative sodium-dependent
bile acid symporter {Arabidopsis thaliana}, partial
(77%)
Length = 1943
Score = 192 bits (487), Expect = 2e-49
Identities = 100/252 (39%), Positives = 155/252 (60%)
Frame = +2
Query: 94 QILSALLPFVVAVTAVAALSQPSTFTWVSKELYAPALGGIMLSIGIGLSMEDFALAFKRP 153
+ L+ L P V + A+ + +P+ TW+ +L+ LG +MLS+G+ L+ +DF + P
Sbjct: 572 ETLTTLFPVWVILGALLGIYKPAAVTWLETDLFTLGLGFLMLSMGLTLTFDDFRRCLRNP 751
Query: 154 LPLSIGFIAQYVLKPVLGVLIAKAFGLPRMFYAGFVLTACVSGAQLSSYASFISKGDVAL 213
+ +GF+AQY++KPVLG IA L G +L +C G Q S+ A++ISKG+VAL
Sbjct: 752 WTVGVGFLAQYLIKPVLGFFIAMTLKLSAPLATGLILVSCCPGGQASNVATYISKGNVAL 931
Query: 214 CIVLTSYTTIASVIVTPLLTGLLIGSVVPVDAVAMSKSILQVVLAPVTLGLLLNTYAKPV 273
+++T+ +TI ++I+TPLLT LL G +VPVDA ++ S QVVL P +G+L N +
Sbjct: 932 SVLMTTCSTIGAIIMTPLLTKLLAGQLVPVDAAGLAISTFQVVLVPTIVGVLANEFFPKF 1111
Query: 274 VSILRPVMPFVAMICTSLCIGSPLAINRSQILSGEGLRLVAPVLIFHAAAFTLGYWFSNL 333
S + V P + +I T+L SP+ +++L +G +L+ PVL HAAAF +GYW S +
Sbjct: 1112TSKIITVTPLIGVILTTLLCASPIG-QVAEVLKTQGAQLILPVLALHAAAFAIGYWMSRI 1288
Query: 334 PSLRFLTSITIN 345
S TS TI+
Sbjct: 1289-SFGESTSRTIS 1321
>BE321838
Length = 214
Score = 135 bits (340), Expect = 3e-32
Identities = 65/65 (100%), Positives = 65/65 (100%)
Frame = +1
Query: 1 MASIPVFSPFTPMKQKHKPLSFQPLLRTRSSFSLQRVSKGCSISSFACSTSKLHGGRVGF 60
MASIPVFSPFTPMKQKHKPLSFQPLLRTRSSFSLQRVSKGCSISSFACSTSKLHGGRVGF
Sbjct: 19 MASIPVFSPFTPMKQKHKPLSFQPLLRTRSSFSLQRVSKGCSISSFACSTSKLHGGRVGF 198
Query: 61 NHREG 65
NHREG
Sbjct: 199NHREG 213
>TC89740 similar to GP|16648877|gb|AAL24290.1 Unknown protein {Arabidopsis
thaliana}, partial (58%)
Length = 965
Score = 124 bits (310), Expect = 8e-29
Identities = 68/183 (37%), Positives = 109/183 (59%)
Frame = +1
Query: 163 QYVLKPVLGVLIAKAFGLPRMFYAGFVLTACVSGAQLSSYASFISKGDVALCIVLTSYTT 222
QY + P+ I+K LP + AG +L C G S+ +++++G+VAL +V+T+ +T
Sbjct: 4 QYSVMPLSAFFISKLLNLPSHYAAGLILVGCCPGGTASNIVTYLARGNVALSVVMTAAST 183
Query: 223 IASVIVTPLLTGLLIGSVVPVDAVAMSKSILQVVLAPVTLGLLLNTYAKPVVSILRPVMP 282
+++VI+TP LT L G V VDA + S LQVVL PV G LLN Y +P+V ++ P+MP
Sbjct: 184 LSAVIMTPFLTAKLAGKYVAVDAAGLLISTLQVVLFPVLAGALLNQYFQPLVKLVSPLMP 363
Query: 283 FVAMICTSLCIGSPLAINRSQILSGEGLRLVAPVLIFHAAAFTLGYWFSNLPSLRFLTSI 342
+A+ ++ G+ +A + S IL G ++A L+ HA+ F GY + + L +S
Sbjct: 364 PLAVATVAVLCGNAIAQSSSAILMSGGQVILASCLL-HASGFFFGYILARMLGLDVASSR 540
Query: 343 TIN 345
TI+
Sbjct: 541 TIS 549
>TC92539 similar to GP|16648877|gb|AAL24290.1 Unknown protein {Arabidopsis
thaliana}, partial (41%)
Length = 689
Score = 118 bits (296), Expect = 3e-27
Identities = 60/170 (35%), Positives = 97/170 (56%), Gaps = 4/170 (2%)
Frame = +1
Query: 88 GERDW----SQILSALLPFVVAVTAVAALSQPSTFTWVSKELYAPALGGIMLSIGIGLSM 143
G R W S+ +S P V + V L +PS+F WV+ +L LG IML +G+ L++
Sbjct: 178 GVRKWIELGSEAISTAFPLWVTIGCVLGLLRPSSFNWVTPKLCTVGLGVIMLGMGMSLTL 357
Query: 144 EDFALAFKRPLPLSIGFIAQYVLKPVLGVLIAKAFGLPRMFYAGFVLTACVSGAQLSSYA 203
+D A P + GF+ QY + P+ L++K LP + AG +L C G S+
Sbjct: 358 DDLRSALFMPKEVISGFVLQYSVMPISAFLVSKLLNLPSHYAAGLILVGCCPGGTASNIV 537
Query: 204 SFISKGDVALCIVLTSYTTIASVIVTPLLTGLLIGSVVPVDAVAMSKSIL 253
+++++G+VAL +++T+ +T+++VI+TP LT L G V VDA + S L
Sbjct: 538 TYLARGNVALSVIMTAASTLSAVIMTPFLTAKLAGKYVAVDAAGLLMSTL 687
>BF632420 weakly similar to GP|21618116|gb| unknown {Arabidopsis thaliana},
partial (17%)
Length = 534
Score = 52.4 bits (124), Expect = 3e-07
Identities = 23/64 (35%), Positives = 39/64 (60%)
Frame = +1
Query: 97 SALLPFVVAVTAVAALSQPSTFTWVSKELYAPALGGIMLSIGIGLSMEDFALAFKRPLPL 156
++LLP + + + AL P + TW + +APALG +M ++G+ S +DF AF RP +
Sbjct: 343 NSLLPPAIVSSTLLALIFPPSLTWFTTRYFAPALGFLMFAVGVNSSEKDFIEAFNRPAEI 522
Query: 157 SIGF 160
+ G+
Sbjct: 523 ATGY 534
>BE319834 similar to GP|21618116|gb unknown {Arabidopsis thaliana}, partial
(26%)
Length = 507
Score = 37.0 bits (84), Expect = 0.013
Identities = 17/42 (40%), Positives = 26/42 (61%)
Frame = +1
Query: 290 SLCIGSPLAINRSQILSGEGLRLVAPVLIFHAAAFTLGYWFS 331
++C G+PLA+N I S G+ ++ V+ FH +AF GY S
Sbjct: 1 AICAGAPLALNVECIKSPLGISILLLVVAFHLSAFIAGYMLS 126
>BG586394 similar to GP|7295463|gb| CG4835 gene product {Drosophila
melanogaster}, partial (2%)
Length = 734
Score = 31.2 bits (69), Expect = 0.69
Identities = 24/99 (24%), Positives = 48/99 (48%), Gaps = 2/99 (2%)
Frame = -1
Query: 160 FIAQYVLKPVLGVLIAKAFGLPRMFYAGFVLTACVSGAQLSSYASFISKGDVALCIVLTS 219
F++ V+ V GV++A +F++GFV VSG ++S + + + ++S
Sbjct: 713 FVSDAVVVSVSGVVVA-------LFFSGFVGVLSVSGCVVASVSGVVVVSFSGFSVGVSS 555
Query: 220 YT--TIASVIVTPLLTGLLIGSVVPVDAVAMSKSILQVV 256
T T+ SV+V + ++ + V ++ S + VV
Sbjct: 554 ETVGTVGSVVVVLFFSDAVVVFGISVSGFSVGVSSVVVV 438
>TC80570 similar to GP|7643796|gb|AAF65513.1| GTP-binding protein {Capsicum
annuum}, partial (35%)
Length = 671
Score = 30.4 bits (67), Expect = 1.2
Identities = 19/55 (34%), Positives = 27/55 (48%)
Frame = -1
Query: 296 PLAINRSQILSGEGLRLVAPVLIFHAAAFTLGYWFSNLPSLRFLTSITINTLSQA 350
P +N I+S V P ++ AAFT S+ S F+TS IN LS++
Sbjct: 407 PETLNLKNIMSPSWTT*VFPSCLYFPAAFTADSLPSSFRSSNFITSAQINPLSKS 243
>TC88542 similar to GP|17065530|gb|AAL32919.1 Unknown protein {Arabidopsis
thaliana}, complete
Length = 1004
Score = 30.4 bits (67), Expect = 1.2
Identities = 22/67 (32%), Positives = 38/67 (55%), Gaps = 2/67 (2%)
Frame = +1
Query: 130 LGGIMLSIGIGLSMEDFALA--FKRPLPLSIGFIAQYVLKPVLGVLIAKAFGLPRMFYAG 187
+ G+ L+IG+ +M+ F FK + IGF+ + P+LG++I +A+G +F +G
Sbjct: 301 VSGVSLTIGLKSTMQFFMKRSNFKGTISFGIGFLILILGWPILGMII-EAYGFIVLF-SG 474
Query: 188 FVLTACV 194
F T V
Sbjct: 475 FWPTLAV 495
>TC76510 homologue to GP|1616609|emb|CAA69931.1 PR10-1 protein {Medicago
truncatula}, partial (97%)
Length = 658
Score = 29.6 bits (65), Expect = 2.0
Identities = 14/39 (35%), Positives = 22/39 (55%), Gaps = 1/39 (2%)
Frame = +1
Query: 53 LHGGRVGFN-HREGKTSFLKFGVDESVVGGGGNGDVGER 90
+ GG ++ H+E + F + S+VGGGG D GE+
Sbjct: 268 VEGGETKYDSHKEDLLDDVNFAYNYSIVGGGGLPDTGEK 384
>TC89433 similar to GP|13277220|emb|CAC34409. ZF-HD homeobox protein
{Flaveria bidentis}, partial (52%)
Length = 1430
Score = 28.5 bits (62), Expect = 4.5
Identities = 9/14 (64%), Positives = 13/14 (92%)
Frame = -1
Query: 81 GGGNGDVGERDWSQ 94
GGG+G++GERDW +
Sbjct: 770 GGGDGELGERDWKR 729
>TC84163 similar to GP|13365608|dbj|BAB39154. avicelase 2 {Humicola
insolens}, partial (3%)
Length = 513
Score = 28.5 bits (62), Expect = 4.5
Identities = 15/49 (30%), Positives = 27/49 (54%), Gaps = 3/49 (6%)
Frame = +2
Query: 6 VFSPFTPMKQKHKPLSFQPLLRTRSS---FSLQRVSKGCSISSFACSTS 51
+FSPF+P K+ P+ +L+ S+ F L +S G + + + ST+
Sbjct: 146 IFSPFSPSPNKYFPIIAS*ILQRNSNNDFFLLSLISAGLGVPTSSSSTN 292
>TC81738 similar to GP|17065530|gb|AAL32919.1 Unknown protein {Arabidopsis
thaliana}, partial (84%)
Length = 665
Score = 28.1 bits (61), Expect = 5.8
Identities = 20/67 (29%), Positives = 37/67 (54%), Gaps = 2/67 (2%)
Frame = +1
Query: 130 LGGIMLSIGIGLSMEDFALA--FKRPLPLSIGFIAQYVLKPVLGVLIAKAFGLPRMFYAG 187
+ G+ L+IG+ +M+ F FK + IGF+ + P+LG +I +++G + ++G
Sbjct: 67 VSGVSLTIGLKSTMQFFMKRSNFKGTVSFGIGFLILILGWPILG-MIVESYGF-LVLFSG 240
Query: 188 FVLTACV 194
F T V
Sbjct: 241 FWPTLSV 261
>BQ149911 GP|13940261|em ATPase subunit 6 {Cebus albifrons}, partial (6%)
Length = 407
Score = 27.7 bits (60), Expect = 7.6
Identities = 14/47 (29%), Positives = 27/47 (56%)
Frame = +2
Query: 202 YASFISKGDVALCIVLTSYTTIASVIVTPLLTGLLIGSVVPVDAVAM 248
Y + + ++A+C V + T + +IV L + ++ VVP+ AVA+
Sbjct: 185 YITVSLRVNIAVCSVYSVSTCVNVIIVLQLSSSVIASRVVPLRAVAV 325
>TC91327 similar to GP|22597168|gb|AAN03471.1 unknown protein {Glycine max},
partial (70%)
Length = 738
Score = 27.7 bits (60), Expect = 7.6
Identities = 19/69 (27%), Positives = 31/69 (44%), Gaps = 4/69 (5%)
Frame = -2
Query: 56 GRVGFNHREGKTSFLKFGVDESVVGGGGNGD----VGERDWSQILSALLPFVVAVTAVAA 111
G +GF+ G T L F + GGGG+ + + QILS + + T +
Sbjct: 362 GSLGFSGSLGLTGSLGFSGSLGLGGGGGSTISMLLMAPQPLQQILSLIFSGLQQTTVIVT 183
Query: 112 LSQPSTFTW 120
+ S++TW
Sbjct: 182 MLFFSSYTW 156
>TC91814 weakly similar to GP|10177113|dbj|BAB10403.
gb|AAD32907.1~gene_id:K2A18.4~strong similarity to
unknown protein {Arabidopsis thaliana}, partial (32%)
Length = 797
Score = 27.7 bits (60), Expect = 7.6
Identities = 24/92 (26%), Positives = 39/92 (42%), Gaps = 7/92 (7%)
Frame = +3
Query: 152 RPLPLSIGFIAQYVLKPVLGVLIAKA---FGL----PRMFYAGFVLTACVSGAQLSSYAS 204
+PL L I + L PV+G+ I KA +G P Y + A +S+ A
Sbjct: 378 KPLTLICIIITRLFLLPVIGLFIVKAAASYGFLPVDPLFQYTLVMQYAMPPAMNISTMAQ 557
Query: 205 FISKGDVALCIVLTSYTTIASVIVTPLLTGLL 236
G+ ++L + A++ +T T LL
Sbjct: 558 LFDVGNEECSVILLGTYSAAAIALTAWSTFLL 653
>TC81105 weakly similar to PIR|F84721|F84721 probable RING zinc finger
protein [imported] - Arabidopsis thaliana, partial (23%)
Length = 683
Score = 27.3 bits (59), Expect = 9.9
Identities = 19/63 (30%), Positives = 31/63 (49%), Gaps = 5/63 (7%)
Frame = +1
Query: 269 YAKPVVSILRPVMPFVA-----MICTSLCIGSPLAINRSQILSGEGLRLVAPVLIFHAAA 323
++ P SI P + F+ MICT L I SPL + S ++ + +R + V++
Sbjct: 145 FSSPRFSIWDPNLFFLLQWTPKMICTMLTIWSPLKMTISTVVIRKKVRWIIIVIMMMMLV 324
Query: 324 FTL 326
TL
Sbjct: 325 ITL 333
>TC84889 similar to GP|21537003|gb|AAM61344.1 unknown {Arabidopsis
thaliana}, partial (29%)
Length = 770
Score = 27.3 bits (59), Expect = 9.9
Identities = 12/26 (46%), Positives = 15/26 (57%)
Frame = +1
Query: 69 FLKFGVDESVVGGGGNGDVGERDWSQ 94
F+ F V S+V GGG G R WS+
Sbjct: 397 FVCFMVFSSLVSGGGTGQTTTRHWSE 474
>BF647972 similar to GP|22532805|gb| C. elegans PQN-75 protein (corresponding
sequence W03D2.1b) {Caenorhabditis elegans}, partial
(3%)
Length = 397
Score = 27.3 bits (59), Expect = 9.9
Identities = 22/62 (35%), Positives = 28/62 (44%), Gaps = 1/62 (1%)
Frame = -2
Query: 54 HGGRVGFNHREGKTSFLKFGVDESVVGGGGNG-DVGERDWSQILSALLPFVVAVTAVAAL 112
HGG G HR G F E V GG G G G R ++ ++ +V V V +L
Sbjct: 375 HGGFGGKXHRSGSGGTNAFMEVEEVGGGSGGGRSDGVR---SVMVEVM*VLVVVGMVVSL 205
Query: 113 SQ 114
SQ
Sbjct: 204 SQ 199
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.324 0.138 0.409
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 11,544,641
Number of Sequences: 36976
Number of extensions: 164715
Number of successful extensions: 1322
Number of sequences better than 10.0: 41
Number of HSP's better than 10.0 without gapping: 1298
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1320
length of query: 379
length of database: 9,014,727
effective HSP length: 98
effective length of query: 281
effective length of database: 5,391,079
effective search space: 1514893199
effective search space used: 1514893199
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)
S2: 59 (27.3 bits)
Medicago: description of AC146343.8