BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146343.4 - phase: 0
(141 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC83096 weakly similar to PIR|T45912|T45912 hypothetical protein... 33 0.029
TC83471 similar to PIR|T45912|T45912 hypothetical protein F5K20.... 29 0.55
TC87516 PIR|T09591|T09591 probable cdc2-like protein kinase cdc2... 28 0.95
TC91609 28 1.2
TC78167 homologue to GP|9294393|dbj|BAB02403.1 mitogen-activated... 28 1.2
BE999879 homologue to GP|17064906|gb Unknown protein {Arabidopsi... 27 2.8
TC80858 similar to GP|4835227|emb|CAB42905.1 glycosyltransferase... 27 3.6
TC76968 weakly similar to GP|2598589|emb|CAA75589.1 MtN19 {Medic... 26 4.7
TC83118 similar to PIR|E96718|E96718 probable cullin T6C23.13 [i... 26 4.7
BE248541 similar to GP|11141605|gb| LEUNIG {Arabidopsis thaliana... 26 4.7
TC78357 similar to GP|15289849|dbj|BAB63546. putative mitogen-ac... 26 6.1
AW686074 similar to PIR|F86392|F863 hypothetical protein AAF9856... 26 6.1
BI268421 similar to PIR|T49132|T491 hypothetical protein F26G5.1... 25 8.0
>TC83096 weakly similar to PIR|T45912|T45912 hypothetical protein F5K20.20 -
Arabidopsis thaliana, partial (21%)
Length = 1227
Score = 33.5 bits (75), Expect = 0.029
Identities = 30/118 (25%), Positives = 55/118 (46%), Gaps = 3/118 (2%)
Frame = +2
Query: 27 STIKNDEFTSWDVMLDDELLKGVKGVY-GSVDNVTYEKVEVENTSDTTEFISDIAIQHDF 85
ST +D + LD+ L K + G+V+ + + V + N + + ++
Sbjct: 761 STSASDNNMENEKELDEVALNEFKTKWLGAVEYIENDTVNIANEVLAIGRVKE----YEL 928
Query: 86 IIVGRRNGI--KSPQTQALASWTEYPELGVLGDLLASPDTNTKASILVVQQQVMPKAS 141
+IVG+ + + + S E+ ELG +GDLL S +S+LV+Q Q + +S
Sbjct: 929 VIVGKGHQLLNSTGMIDIKDSQLEHAELGPIGDLLTSSAQGITSSVLVIQGQHLINSS 1102
>TC83471 similar to PIR|T45912|T45912 hypothetical protein F5K20.20 -
Arabidopsis thaliana, partial (18%)
Length = 983
Score = 29.3 bits (64), Expect = 0.55
Identities = 17/53 (32%), Positives = 27/53 (50%)
Frame = +3
Query: 83 HDFIIVGRRNGIKSPQTQALASWTEYPELGVLGDLLASPDTNTKASILVVQQQ 135
+D I+VG+ + + E+ ELG +GD+L S + AS + V QQ
Sbjct: 654 YDLIVVGKGRFPSTMVAELAEREAEHAELGPIGDILTSSMGHKMASSVFVIQQ 812
>TC87516 PIR|T09591|T09591 probable cdc2-like protein kinase cdc2MsF -
alfalfa, complete
Length = 1251
Score = 28.5 bits (62), Expect = 0.95
Identities = 21/72 (29%), Positives = 31/72 (42%), Gaps = 3/72 (4%)
Frame = +2
Query: 44 ELLKGVKGVYGSVDNVTYEKVEVENTSDTTEFISDIAIQ---HDFIIVGRRNGIKSPQTQ 100
E+L G +VD + + E + T F D +Q H F ++G N P
Sbjct: 641 EVLLGATHYSMAVDMWSVACIFAELVTKTALFPGDSELQQLLHIFRLLGTPNEDVWPGVS 820
Query: 101 ALASWTEYPELG 112
L +W EYP+ G
Sbjct: 821 KLMNWHEYPQWG 856
>TC91609
Length = 996
Score = 28.1 bits (61), Expect = 1.2
Identities = 10/29 (34%), Positives = 19/29 (65%)
Frame = -2
Query: 4 KHCVWLKGQSKKILITWVVYHLVSTIKND 32
K+ WL G+S K+ I + +H++S + N+
Sbjct: 482 KYRTWLSGRSSKLRIMSIEFHILSFLPNE 396
>TC78167 homologue to GP|9294393|dbj|BAB02403.1 mitogen-activated protein
kinase {Arabidopsis thaliana}, partial (36%)
Length = 927
Score = 28.1 bits (61), Expect = 1.2
Identities = 23/86 (26%), Positives = 42/86 (48%), Gaps = 14/86 (16%)
Frame = +1
Query: 7 VWLKGQSKKIL---------ITWVVYHLVSTIKNDEFTSWDVMLDDELLKGV-KGVYG-- 54
+W+ Q KK+L +T + L ++ + + FT + ++++ + KG YG
Sbjct: 187 IWVSLQCKKLLSAF*KKRRTMTQKIQQLKNSKELEFFTEYGEANRYKIIEIIGKGSYGVV 366
Query: 55 --SVDNVTYEKVEVENTSDTTEFISD 78
++D T EKV ++ D E ISD
Sbjct: 367 CAAIDTHTGEKVAIKKIQDAFEHISD 444
>BE999879 homologue to GP|17064906|gb Unknown protein {Arabidopsis thaliana},
partial (20%)
Length = 659
Score = 26.9 bits (58), Expect = 2.8
Identities = 20/57 (35%), Positives = 30/57 (52%), Gaps = 5/57 (8%)
Frame = +2
Query: 27 STIKNDEFTSWD----VMLDDELLKGVKGVYGSV-DNVTYEKVEVENTSDTTEFISD 78
S+I D FT + M+++ + KG GV S D T EKV ++ +D E +SD
Sbjct: 335 SSIDVDFFTEYGEGSRYMIEEVIGKGSYGVVCSAYDTHTGEKVAIKKINDIFEHVSD 505
>TC80858 similar to GP|4835227|emb|CAB42905.1 glycosyltransferase-like protein
{Arabidopsis thaliana}, partial (80%)
Length = 1509
Score = 26.6 bits (57), Expect = 3.6
Identities = 9/30 (30%), Positives = 19/30 (63%)
Frame = +2
Query: 7 VWLKGQSKKILITWVVYHLVSTIKNDEFTS 36
+WL +S + ++ + +HL+ + N EFT+
Sbjct: 1295 IWLTVKSGR*VVVYYYFHLLGEVSNCEFTT 1384
>TC76968 weakly similar to GP|2598589|emb|CAA75589.1 MtN19 {Medicago
truncatula}, partial (39%)
Length = 647
Score = 26.2 bits (56), Expect = 4.7
Identities = 14/36 (38%), Positives = 21/36 (57%)
Frame = -1
Query: 13 SKKILITWVVYHLVSTIKNDEFTSWDVMLDDELLKG 48
S K+ +T ++Y L TIK+ + T + V L LL G
Sbjct: 224 SFKLPVTSIIYILTGTIKSSQSTHFTVHLK*RLLLG 117
>TC83118 similar to PIR|E96718|E96718 probable cullin T6C23.13 [imported] -
Arabidopsis thaliana, partial (35%)
Length = 902
Score = 26.2 bits (56), Expect = 4.7
Identities = 15/35 (42%), Positives = 22/35 (62%)
Frame = +2
Query: 30 KNDEFTSWDVMLDDELLKGVKGVYGSVDNVTYEKV 64
++ EF S + +DD+L KG+KGV VT +KV
Sbjct: 242 RSPEFIS--LFVDDKLRKGLKGVNEDDVEVTLDKV 340
>BE248541 similar to GP|11141605|gb| LEUNIG {Arabidopsis thaliana}, partial
(10%)
Length = 376
Score = 26.2 bits (56), Expect = 4.7
Identities = 11/25 (44%), Positives = 15/25 (60%)
Frame = -2
Query: 100 QALASWTEYPELGVLGDLLASPDTN 124
Q ASW +P+ +GDLL P T+
Sbjct: 78 QIFASWLRHPKNPSVGDLLTFPHTS 4
>TC78357 similar to GP|15289849|dbj|BAB63546. putative mitogen-activated
protein kinase {Oryza sativa (japonica cultivar-group)},
partial (69%)
Length = 1751
Score = 25.8 bits (55), Expect = 6.1
Identities = 15/35 (42%), Positives = 19/35 (53%), Gaps = 4/35 (11%)
Frame = +3
Query: 50 KGVYG----SVDNVTYEKVEVENTSDTTEFISDIA 80
KG YG ++D T EKV ++ D E ISD A
Sbjct: 555 KGSYGVVCSAIDTHTGEKVAIKKIHDIFEHISDAA 659
>AW686074 similar to PIR|F86392|F863 hypothetical protein AAF98564.1
[imported] - Arabidopsis thaliana, partial (18%)
Length = 651
Score = 25.8 bits (55), Expect = 6.1
Identities = 11/34 (32%), Positives = 17/34 (49%)
Frame = +2
Query: 51 GVYGSVDNVTYEKVEVENTSDTTEFISDIAIQHD 84
GVYG+ +TYE V + + + DI + D
Sbjct: 458 GVYGNTLKITYEHVFISTLKQSNK*YEDIQLMKD 559
>BI268421 similar to PIR|T49132|T491 hypothetical protein F26G5.110 -
Arabidopsis thaliana, partial (16%)
Length = 487
Score = 25.4 bits (54), Expect = 8.0
Identities = 10/36 (27%), Positives = 19/36 (52%)
Frame = +2
Query: 7 VWLKGQSKKILITWVVYHLVSTIKNDEFTSWDVMLD 42
+W+ Q ++I ++V L + D+ WD+ LD
Sbjct: 35 LWIAEQICGLVILYLVIQL*GMVNQDQVLDWDMDLD 142
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.316 0.133 0.387
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 4,162,071
Number of Sequences: 36976
Number of extensions: 44797
Number of successful extensions: 247
Number of sequences better than 10.0: 26
Number of HSP's better than 10.0 without gapping: 245
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 247
length of query: 141
length of database: 9,014,727
effective HSP length: 86
effective length of query: 55
effective length of database: 5,834,791
effective search space: 320913505
effective search space used: 320913505
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 53 (25.0 bits)
Medicago: description of AC146343.4