BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146343.12 + phase: 0 /pseudo
(176 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC85253 homologue to SP|Q9M3V8|RS6_ASPOF 40S ribosomal protein S... 353 3e-98
TC85232 homologue to SP|Q9M3V8|RS6_ASPOF 40S ribosomal protein S... 346 2e-96
TC85250 homologue to SP|Q9M3V8|RS6_ASPOF 40S ribosomal protein S... 343 2e-95
TC84617 similar to GP|1151236|gb|AAB68209.1| Lpg18p {Saccharomyc... 191 1e-49
BG458077 similar to SP|Q9M3V8|RS6_ 40S ribosomal protein S6. [Ga... 187 2e-48
BQ137345 homologue to PIR|T05100|T051 ribosomal protein S6 cyto... 95 1e-23
TC85235 homologue to GP|3413170|emb|CAA09042.1 40S ribosomal pro... 96 1e-20
TC87578 similar to PIR|T12113|T12113 transcription factor - fava... 28 1.4
CB065339 weakly similar to PIR|H83604|H836 hypothetical protein ... 28 2.5
TC82424 similar to PIR|T51283|T51283 glucan 1 3-beta-glucosidase... 27 4.2
BQ139363 similar to GP|15982866|gb| At1g27210/T7N9_27 {Arabidops... 27 4.2
BQ140761 weakly similar to GP|18076245|emb phosphophoryn {Rattus... 27 5.5
TC81787 similar to PIR|C96829|C96829 unknown protein F19K16.21 [... 27 5.5
TC88403 similar to GP|15010642|gb|AAK73980.1 At1g59710/T30E16_31... 26 9.3
AW684108 26 9.3
>TC85253 homologue to SP|Q9M3V8|RS6_ASPOF 40S ribosomal protein S6. [Garden
asparagus] {Asparagus officinalis}, partial (97%)
Length = 1051
Score = 353 bits (905), Expect = 3e-98
Identities = 170/174 (97%), Positives = 172/174 (98%)
Frame = +1
Query: 1 FNVANPTTGCQKKLEIDDDQKLRAFWDKRISQEVNGDALGEEFKGYVFKITGGCDKQGFP 60
FNVANPTTGCQKKLEIDDDQKLRAFWDKRISQEVNGDALGEEFKGYVFKITGGCDKQGFP
Sbjct: 79 FNVANPTTGCQKKLEIDDDQKLRAFWDKRISQEVNGDALGEEFKGYVFKITGGCDKQGFP 258
Query: 61 MKQGVLTPGRVRLLLHRGTPCFRGHGRRNGERRRKSVRGCIVSPDLSVLNLVIVKKGEND 120
MKQGVLTPGRVRLLLHRGTPCFRGHGRRNGERRRKSVRGCIVSPDLSVLNLVIVKKGEND
Sbjct: 259 MKQGVLTPGRVRLLLHRGTPCFRGHGRRNGERRRKSVRGCIVSPDLSVLNLVIVKKGEND 438
Query: 121 LPGLTDVEKPRMRGPKRASKIRKLFNLSKDDDVRKYVNTYRRTFTTKDGAKLPR 174
LPGLTDVEKPRMRGPKRASKIRKLFNLSKDDDVRKYVNTYRRTFTTKDG K+ +
Sbjct: 439 LPGLTDVEKPRMRGPKRASKIRKLFNLSKDDDVRKYVNTYRRTFTTKDGKKVSK 600
>TC85232 homologue to SP|Q9M3V8|RS6_ASPOF 40S ribosomal protein S6. [Garden
asparagus] {Asparagus officinalis}, partial (96%)
Length = 1154
Score = 346 bits (888), Expect = 2e-96
Identities = 167/174 (95%), Positives = 170/174 (96%)
Frame = +2
Query: 1 FNVANPTTGCQKKLEIDDDQKLRAFWDKRISQEVNGDALGEEFKGYVFKITGGCDKQGFP 60
FNVANPTTGCQKKLEIDDD KLRAFWDKRISQEV GDALGEEFKGYVFKITGGCDKQGFP
Sbjct: 53 FNVANPTTGCQKKLEIDDDMKLRAFWDKRISQEVLGDALGEEFKGYVFKITGGCDKQGFP 232
Query: 61 MKQGVLTPGRVRLLLHRGTPCFRGHGRRNGERRRKSVRGCIVSPDLSVLNLVIVKKGEND 120
MKQGVLTPGRVRLLLHRGTPCFRGHGRRNGERRRKSVRGCIVSPDLSVLNLVIVKKG+ND
Sbjct: 233 MKQGVLTPGRVRLLLHRGTPCFRGHGRRNGERRRKSVRGCIVSPDLSVLNLVIVKKGDND 412
Query: 121 LPGLTDVEKPRMRGPKRASKIRKLFNLSKDDDVRKYVNTYRRTFTTKDGAKLPR 174
LPGLTDVEKPRMRGPKRASKIRKLFNLSKDDDVRKYVNTYRRTFTTKDG K+ +
Sbjct: 413 LPGLTDVEKPRMRGPKRASKIRKLFNLSKDDDVRKYVNTYRRTFTTKDGKKVSK 574
>TC85250 homologue to SP|Q9M3V8|RS6_ASPOF 40S ribosomal protein S6. [Garden
asparagus] {Asparagus officinalis}, partial (96%)
Length = 1009
Score = 343 bits (881), Expect = 2e-95
Identities = 167/171 (97%), Positives = 167/171 (97%)
Frame = +2
Query: 1 FNVANPTTGCQKKLEIDDDQKLRAFWDKRISQEVNGDALGEEFKGYVFKITGGCDKQGFP 60
FNVANPTTGCQKKLEIDDD KLRAFWDKRISQEV GDALGEEFKGYVFKITGGCDKQGFP
Sbjct: 56 FNVANPTTGCQKKLEIDDDMKLRAFWDKRISQEVLGDALGEEFKGYVFKITGGCDKQGFP 235
Query: 61 MKQGVLTPGRVRLLLHRGTPCFRGHGRRNGERRRKSVRGCIVSPDLSVLNLVIVKKGEND 120
MKQGVLTPGRVRLLLHRGTPCFRGHGRRNGERRRKSVRGCIVSPDLSVLNLVIVKKGEND
Sbjct: 236 MKQGVLTPGRVRLLLHRGTPCFRGHGRRNGERRRKSVRGCIVSPDLSVLNLVIVKKGEND 415
Query: 121 LPGLTDVEKPRMRGPKRASKIRKLFNLSKDDDVRKYVNTYRRTFTTKDGAK 171
LPGLTDVEKPRMRGPKRASKIRKLFNLSKDDDVRKYVNTYRRTFTTK G K
Sbjct: 416 LPGLTDVEKPRMRGPKRASKIRKLFNLSKDDDVRKYVNTYRRTFTTKAGKK 568
>TC84617 similar to GP|1151236|gb|AAB68209.1| Lpg18p {Saccharomyces
cerevisiae}, partial (86%)
Length = 769
Score = 191 bits (486), Expect = 1e-49
Identities = 98/157 (62%), Positives = 123/157 (77%)
Frame = +1
Query: 20 QKLRAFWDKRISQEVNGDALGEEFKGYVFKITGGCDKQGFPMKQGVLTPGRVRLLLHRGT 79
++LR F+DKRIS +V+GD++G+E+KGY+F+++GG DKQGFPMKQGVL RVRLLL +G
Sbjct: 1 RQLRVFYDKRISADVSGDSIGDEYKGYIFRVSGGNDKQGFPMKQGVLRNDRVRLLLSKGH 180
Query: 80 PCFRGHGRRNGERRRKSVRGCIVSPDLSVLNLVIVKKGENDLPGLTDVEKPRMRGPKRAS 139
C+R RR GER+RKSVRGCIV DL+VL++VIVK+GE +PGLTD P+ GPKRAS
Sbjct: 181 SCYRP--RRTGERKRKSVRGCIVGNDLAVLSVVIVKQGEQPIPGLTDNTIPKRLGPKRAS 354
Query: 140 KIRKLFNLSKDDDVRKYVNTYRRTFTTKDGAKLPRYK 176
KIRK FNLSK+DDVRK+V RR T K+ K P K
Sbjct: 355 KIRKFFNLSKEDDVRKFV--IRREITPKNQNKKPYTK 459
>BG458077 similar to SP|Q9M3V8|RS6_ 40S ribosomal protein S6. [Garden
asparagus] {Asparagus officinalis}, partial (44%)
Length = 459
Score = 187 bits (474), Expect = 2e-48
Identities = 89/109 (81%), Positives = 98/109 (89%)
Frame = +3
Query: 2 NVANPTTGCQKKLEIDDDQKLRAFWDKRISQEVNGDALGEEFKGYVFKITGGCDKQGFPM 61
N+A P+TGCQKKLEIDD+ KLRAF+DKR+S E+ GD LGEEFKGYVFKI+GG DKQGFPM
Sbjct: 132 NIAYPSTGCQKKLEIDDEAKLRAFYDKRVSAEIEGDVLGEEFKGYVFKISGGQDKQGFPM 311
Query: 62 KQGVLTPGRVRLLLHRGTPCFRGHGRRNGERRRKSVRGCIVSPDLSVLN 110
KQGVLT GRVRLL+ G CFRG+GRRNGERRRKSVRGCIVSPDLSVLN
Sbjct: 312 KQGVLTNGRVRLLMTPGDQCFRGYGRRNGERRRKSVRGCIVSPDLSVLN 458
>BQ137345 homologue to PIR|T05100|T051 ribosomal protein S6 cytosolic -
Arabidopsis thaliana, partial (19%)
Length = 951
Score = 94.7 bits (234), Expect(2) = 1e-23
Identities = 44/46 (95%), Positives = 44/46 (95%)
Frame = +2
Query: 1 FNVANPTTGCQKKLEIDDDQKLRAFWDKRISQEVNGDALGEEFKGY 46
FNVANPTTGCQKKLEIDDD KLRAFWDKRISQEV GDALGEEFKGY
Sbjct: 71 FNVANPTTGCQKKLEIDDDMKLRAFWDKRISQEVLGDALGEEFKGY 208
Score = 31.2 bits (69), Expect(2) = 1e-23
Identities = 13/15 (86%), Positives = 13/15 (86%)
Frame = +3
Query: 48 FKITGGCDKQGFPMK 62
FKITGGCD Q FPMK
Sbjct: 213 FKITGGCDLQ*FPMK 257
>TC85235 homologue to GP|3413170|emb|CAA09042.1 40S ribosomal protein S6
{Cicer arietinum}, partial (54%)
Length = 582
Score = 95.5 bits (236), Expect = 1e-20
Identities = 45/49 (91%), Positives = 47/49 (95%)
Frame = +1
Query: 126 DVEKPRMRGPKRASKIRKLFNLSKDDDVRKYVNTYRRTFTTKDGAKLPR 174
DVEKPRMRGPKRASKIRKLFNLSKDDDVRKYVNTYRRTFTTKDG K+ +
Sbjct: 1 DVEKPRMRGPKRASKIRKLFNLSKDDDVRKYVNTYRRTFTTKDGKKVSK 147
>TC87578 similar to PIR|T12113|T12113 transcription factor - fava bean,
partial (49%)
Length = 1021
Score = 28.5 bits (62), Expect = 1.4
Identities = 18/44 (40%), Positives = 22/44 (49%)
Frame = +2
Query: 4 ANPTTGCQKKLEIDDDQKLRAFWDKRISQEVNGDALGEEFKGYV 47
A PTTG K LE DDD + + RI E D EE + +V
Sbjct: 431 AQPTTGVAKVLEGDDDDAVDPHLE-RIRNEAGEDESDEEDEDFV 559
>CB065339 weakly similar to PIR|H83604|H836 hypothetical protein PA0319
[imported] - Pseudomonas aeruginosa (strain PAO1),
partial (25%)
Length = 630
Score = 27.7 bits (60), Expect = 2.5
Identities = 18/54 (33%), Positives = 23/54 (42%)
Frame = +3
Query: 51 TGGCDKQGFPMKQGVLTPGRVRLLLHRGTPCFRGHGRRNGERRRKSVRGCIVSP 104
TG C + PG+ LHR PC R R RR ++VR C +P
Sbjct: 225 TGSCPSRQTACGSWD*RPGQRPHRLHRRRPCRRYPHDRPATRRFRAVRPCSRNP 386
>TC82424 similar to PIR|T51283|T51283 glucan 1 3-beta-glucosidase (EC
3.2.1.58) [imported] - common tobacco, partial (45%)
Length = 962
Score = 26.9 bits (58), Expect = 4.2
Identities = 15/32 (46%), Positives = 21/32 (64%)
Frame = +2
Query: 110 NLVIVKKGENDLPGLTDVEKPRMRGPKRASKI 141
+LV++K GEN V+KP + PK+ASKI
Sbjct: 218 SLVLLKNGEN-------VDKPILPLPKKASKI 292
>BQ139363 similar to GP|15982866|gb| At1g27210/T7N9_27 {Arabidopsis
thaliana}, partial (13%)
Length = 461
Score = 26.9 bits (58), Expect = 4.2
Identities = 15/38 (39%), Positives = 18/38 (46%)
Frame = -3
Query: 63 QGVLTPGRVRLLLHRGTPCFRGHGRRNGERRRKSVRGC 100
+GV T G RL + H RR RRR V+GC
Sbjct: 177 EGVAT*GHCRLFVR----LVSRHSRRRSRRRRTVVKGC 76
>BQ140761 weakly similar to GP|18076245|emb phosphophoryn {Rattus
norvegicus}, partial (9%)
Length = 620
Score = 26.6 bits (57), Expect = 5.5
Identities = 14/34 (41%), Positives = 20/34 (58%)
Frame = +3
Query: 62 KQGVLTPGRVRLLLHRGTPCFRGHGRRNGERRRK 95
+QG P R+R L H+ R HGRR E+R++
Sbjct: 156 RQGREHPHRLRRLGHQAGRPVRCHGRRREEQRQQ 257
>TC81787 similar to PIR|C96829|C96829 unknown protein F19K16.21 [imported] -
Arabidopsis thaliana, partial (9%)
Length = 703
Score = 26.6 bits (57), Expect = 5.5
Identities = 17/46 (36%), Positives = 22/46 (46%)
Frame = +2
Query: 78 GTPCFRGHGRRNGERRRKSVRGCIVSPDLSVLNLVIVKKGENDLPG 123
GT C G+ R G +R S C PD +NL+ KKG + G
Sbjct: 185 GTSC--GYCRFKGNVQRTSQLTCE*DPDNEFINLI*PKKGLGERKG 316
>TC88403 similar to GP|15010642|gb|AAK73980.1 At1g59710/T30E16_31
{Arabidopsis thaliana}, partial (33%)
Length = 1143
Score = 25.8 bits (55), Expect = 9.3
Identities = 19/69 (27%), Positives = 30/69 (42%), Gaps = 2/69 (2%)
Frame = +1
Query: 78 GTPCFRGHGRRNGERRRKSVRGCIVSPDLSVLNLVIVKKGENDLPGLTDVEKPRMRG--P 135
G C HGR + R +G VSP+L L+ +++ D++ R G P
Sbjct: 208 GVTCDERHGRVHSLILRNMSQGGTVSPNLGNLSFLVI----------LDLKNNRFGGQFP 357
Query: 136 KRASKIRKL 144
K +R+L
Sbjct: 358 KELCSLRRL 384
>AW684108
Length = 447
Score = 25.8 bits (55), Expect = 9.3
Identities = 15/41 (36%), Positives = 22/41 (53%), Gaps = 1/41 (2%)
Frame = -2
Query: 86 GRRNGERRRKSVRGCIVSPDLSVLNLVIVKKGEN-DLPGLT 125
G+ R+ +S GC V PD + L + KK ++ DL LT
Sbjct: 356 GKXVQNRKNRS*AGCEVRPDFDFIKLXLKKKKKSPDL*ALT 234
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.320 0.141 0.426
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 4,939,193
Number of Sequences: 36976
Number of extensions: 61166
Number of successful extensions: 303
Number of sequences better than 10.0: 30
Number of HSP's better than 10.0 without gapping: 302
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 302
length of query: 176
length of database: 9,014,727
effective HSP length: 90
effective length of query: 86
effective length of database: 5,686,887
effective search space: 489072282
effective search space used: 489072282
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 55 (25.8 bits)
Medicago: description of AC146343.12