BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146332.8 - phase: 0 /pseudo
(199 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BQ139298 similar to GP|12322915|gb| unknown protein; 103163-1060... 144 2e-35
TC87385 similar to GP|23093788|gb|AAF50220.2 CG16711-PA {Drosoph... 32 0.16
TC87384 32 0.16
TC86301 similar to GP|8809622|dbj|BAA97173.1 Myb-related transcr... 31 0.28
BI264152 weakly similar to SP|Q01373|FOX2_ Peroxisomal hydratase... 30 0.47
TC88229 similar to PIR|E84471|E84471 probable beta-1 3-glucanase... 30 0.81
TC83939 similar to GP|10178016|dbj|BAB11468. gb|AAF24581.1~gene_... 29 1.1
BG645718 29 1.4
TC87534 similar to SP|P17597|ILVB_ARATH Acetolactate synthase c... 28 1.8
TC87445 similar to GP|13937224|gb|AAK50104.1 At1g74910/F9E10_24 ... 28 1.8
TC80976 similar to PIR|T41746|T41746 ribosomal protein L11 homol... 28 1.8
CA918257 weakly similar to GP|21689863|gb| unknown protein {Arab... 28 1.8
CA917313 weakly similar to GP|10178016|dbj gb|AAF24581.1~gene_id... 28 2.3
TC79276 similar to PIR|F86161|F86161 hypothetical protein AAD258... 28 3.1
BQ137234 similar to EGAD|35719|3709 hypothetical protein {Burkho... 28 3.1
TC90573 similar to GP|15081594|gb|AAK82651.1 RSH-like protein {C... 27 4.0
TC92439 similar to PIR|T06775|T06775 phosphoinositide-specific p... 27 5.2
TC85211 PIR|S22489|S22489 IgE-dependent histamine-releasing fact... 27 5.2
CA919642 similar to PIR|E85433|E854 SCARECROW-like protein [impo... 27 6.8
BQ750912 similar to GP|7291124|gb| CG15311 gene product {Drosoph... 27 6.8
>BQ139298 similar to GP|12322915|gb| unknown protein; 103163-106072
{Arabidopsis thaliana}, partial (7%)
Length = 657
Score = 144 bits (363), Expect = 2e-35
Identities = 93/167 (55%), Positives = 110/167 (65%), Gaps = 19/167 (11%)
Frame = +2
Query: 1 MSSCQTK---HLKQ-----KHNKIRKRGCSSTSSSSPFSRRYRFKRAILMGKKSGYNTPA 52
MSSC + +LK+ HNKIRKR CSS+ SSS +RRYRFKRAIL+GKK G TP
Sbjct: 170 MSSCHNQTNNNLKEGFREISHNKIRKRNCSSSXSSS-LARRYRFKRAILVGKKGGSTTPV 346
Query: 53 PMWKTS-SKSPSMATHHHHAMKKTALHSSRTGLPFKEK-KEISVSARKLAATLWEINDLT 110
P+WKTS + SPSM H L+SS +GLP K+K KE+SVSARKLAA+LWEINDL
Sbjct: 347 PLWKTSTTSSPSMENTTQH-----LLYSSASGLPSKDKEKEVSVSARKLAASLWEINDLH 511
Query: 111 PSRIKK----ESMKSNKE-----RDKVERLCRSVLLGPQKLDPLVSP 148
PSR+KK E M+S KE R+K + L RS LL P DP SP
Sbjct: 512 PSRVKKEFEAEQMRSCKETSXRSREKAKSLXRSGLLXPLMSDPCNSP 652
>TC87385 similar to GP|23093788|gb|AAF50220.2 CG16711-PA {Drosophila
melanogaster}, partial (1%)
Length = 879
Score = 32.0 bits (71), Expect = 0.16
Identities = 17/46 (36%), Positives = 25/46 (53%), Gaps = 5/46 (10%)
Frame = +2
Query: 58 SSKSPSMAT---HHHHAMKKTALHSSRTG--LPFKEKKEISVSARK 98
SS +PS A HHHH +K + H S +PF ++ +S+ A K
Sbjct: 44 SSSNPSAAAASNHHHHTIKSSYQHESPLSNLIPFVLRRSLSIHAEK 181
>TC87384
Length = 740
Score = 32.0 bits (71), Expect = 0.16
Identities = 17/46 (36%), Positives = 25/46 (53%), Gaps = 5/46 (10%)
Frame = +3
Query: 58 SSKSPSMAT---HHHHAMKKTALHSSRTG--LPFKEKKEISVSARK 98
SS +PS A HHHH +K + H S +PF ++ +S+ A K
Sbjct: 69 SSSNPSAAAASNHHHHTIKSSYQHESPLSNLIPFVLRRSLSIHAEK 206
>TC86301 similar to GP|8809622|dbj|BAA97173.1 Myb-related transcription
activator-like {Arabidopsis thaliana}, partial (56%)
Length = 1396
Score = 31.2 bits (69), Expect = 0.28
Identities = 22/86 (25%), Positives = 32/86 (36%)
Frame = +2
Query: 51 PAPMWKTSSKSPSMATHHHHAMKKTALHSSRTGLPFKEKKEISVSARKLAATLWEINDLT 110
P P W S P+ H +K T +HS P + + +S L T+ + T
Sbjct: 914 PLPYWSGYSPEPAPKKETHEVVKPTPVHSKS---PINVDELVGMSKLSLGETIGDAGPST 1084
Query: 111 PSRIKKESMKSNKERDKVERLCRSVL 136
SR E S + C +VL
Sbjct: 1085LSRKLLEEGPSRQSAFHTTPACGTVL 1162
>BI264152 weakly similar to SP|Q01373|FOX2_ Peroxisomal
hydratase-dehydrogenase-epimerase (HDE)
(Multifunctional beta-oxidation protein) (MFP), partial
(3%)
Length = 659
Score = 30.4 bits (67), Expect = 0.47
Identities = 15/28 (53%), Positives = 18/28 (63%)
Frame = +1
Query: 2 SSCQTKHLKQKHNKIRKRGCSSTSSSSP 29
SSCQ L + +R RGCS+ SSSSP
Sbjct: 19 SSCQVPALALSTHLLR*RGCSAMSSSSP 102
>TC88229 similar to PIR|E84471|E84471 probable beta-1 3-glucanase [imported]
- Arabidopsis thaliana, partial (69%)
Length = 1368
Score = 29.6 bits (65), Expect = 0.81
Identities = 18/53 (33%), Positives = 28/53 (51%), Gaps = 1/53 (1%)
Frame = -1
Query: 10 KQKHNKIRKRGCSSTSSSSPFSRRYRFKRAILMGK-KSGYNTPAPMWKTSSKS 61
+Q+ + R+RG S++ FSRR +F ++ G SG N P W S+S
Sbjct: 666 RQRLTRKRRRGKR*PSNTKTFSRR-KFNIGLITGSMSSGLNDPVERWVRGSES 511
>TC83939 similar to GP|10178016|dbj|BAB11468.
gb|AAF24581.1~gene_id:MBK23.17~strong similarity to
unknown protein {Arabidopsis thaliana}, partial (24%)
Length = 734
Score = 29.3 bits (64), Expect = 1.1
Identities = 13/19 (68%), Positives = 14/19 (73%)
Frame = +1
Query: 93 SVSARKLAATLWEINDLTP 111
+VSARKLAA LWE N P
Sbjct: 124 TVSARKLAAALWEFNHYFP 180
>BG645718
Length = 756
Score = 28.9 bits (63), Expect = 1.4
Identities = 12/36 (33%), Positives = 20/36 (55%), Gaps = 2/36 (5%)
Frame = +2
Query: 65 ATHHHHAMKKTALHSSRTG--LPFKEKKEISVSARK 98
+ HHHH +K + H S +PF ++ +S+ A K
Sbjct: 14 SNHHHHTIKSSYQHESPLSNLIPFVLRRSLSIHAEK 121
>TC87534 similar to SP|P17597|ILVB_ARATH Acetolactate synthase chloroplast
precursor (EC 4.1.3.18) (Acetohydroxy-acid synthase)
(ALS)., partial (87%)
Length = 2258
Score = 28.5 bits (62), Expect = 1.8
Identities = 12/33 (36%), Positives = 16/33 (48%)
Frame = +3
Query: 56 KTSSKSPSMATHHHHAMKKTALHSSRTGLPFKE 88
K S+ PS+ HH+H KT S T P +
Sbjct: 75 KPRSQPPSLPLHHNHFQTKTTFSESLTHFPLTQ 173
>TC87445 similar to GP|13937224|gb|AAK50104.1 At1g74910/F9E10_24 {Arabidopsis
thaliana}, partial (91%)
Length = 1622
Score = 28.5 bits (62), Expect = 1.8
Identities = 16/58 (27%), Positives = 27/58 (45%)
Frame = -1
Query: 9 LKQKHNKIRKRGCSSTSSSSPFSRRYRFKRAILMGKKSGYNTPAPMWKTSSKSPSMAT 66
+K K N ++ + + S S R +L G+ + N P P T++ SPS+ T
Sbjct: 1481 IKHKANTLKIKSQEQRTGSIKESHPERRTFKVLCGRTNATNNPLPHPSTATDSPSIVT 1308
>TC80976 similar to PIR|T41746|T41746 ribosomal protein L11 homolog -
Arabidopsis thaliana, partial (92%)
Length = 890
Score = 28.5 bits (62), Expect = 1.8
Identities = 12/23 (52%), Positives = 16/23 (69%)
Frame = -3
Query: 17 RKRGCSSTSSSSPFSRRYRFKRA 39
R+R CSS+ +S P RRY F+ A
Sbjct: 195 RRRTCSSSWNSQPNCRRYHFRNA 127
>CA918257 weakly similar to GP|21689863|gb| unknown protein {Arabidopsis
thaliana}, partial (19%)
Length = 739
Score = 28.5 bits (62), Expect = 1.8
Identities = 31/122 (25%), Positives = 52/122 (42%), Gaps = 9/122 (7%)
Frame = +3
Query: 22 SSTSSSSPFSRRYRFKRAILMGKKSGYN-TPAPMW-KTSSKSPSMATHHHHAMKKTALHS 79
S+TSSSS + I + YN + W KTSS S S++TH + K AL
Sbjct: 165 STTSSSSNLPSSSTSEVPITESRSYIYNNSKQKQWSKTSSSSSSISTHSSLSSLKHALPE 344
Query: 80 SRTGLPFKEKKEIS-------VSARKLAATLWEINDLTPSRIKKESMKSNKERDKVERLC 132
+ PF + + +S+ +L + + R + + + RD++ +C
Sbjct: 345 NPHIYPFSDISSATNNFSANRLSSNSFRCSLHNRDVVVFQRKFRRQIHLPELRDRLALIC 524
Query: 133 RS 134
RS
Sbjct: 525 RS 530
>CA917313 weakly similar to GP|10178016|dbj
gb|AAF24581.1~gene_id:MBK23.17~strong similarity to
unknown protein {Arabidopsis thaliana}, partial (9%)
Length = 620
Score = 28.1 bits (61), Expect = 2.3
Identities = 12/32 (37%), Positives = 19/32 (58%)
Frame = +3
Query: 85 PFKEKKEISVSARKLAATLWEINDLTPSRIKK 116
P ++ +S SARKL A LW+I +++ K
Sbjct: 66 PSQQNSIVSTSARKLCANLWQIQHTPFAKMNK 161
>TC79276 similar to PIR|F86161|F86161 hypothetical protein AAD25801.1
[imported] - Arabidopsis thaliana, partial (3%)
Length = 1748
Score = 27.7 bits (60), Expect = 3.1
Identities = 27/109 (24%), Positives = 45/109 (40%), Gaps = 7/109 (6%)
Frame = +3
Query: 54 MWKTSSKSPSMAT-----HHHHAMKKTALHSSRTGLPFKEKKEISVSARKLAATLWEIND 108
+W+TS K+P MA HH K + +G E KE+ V +++ +
Sbjct: 867 LWETSDKTPKMAAEPAEDHHQRRASKETYNKHPSGDSLVE-KELGVDKLEISRRM----- 1028
Query: 109 LTPSRIKKESMKSN--KERDKVERLCRSVLLGPQKLDPLVSPFSERTKG 155
SR ++E KS + D + ++ + Q L V + TKG
Sbjct: 1029---SRPREEGNKSRVLERLDSDSQKLTNLQITIQDLMKKVETIEKSTKG 1166
>BQ137234 similar to EGAD|35719|3709 hypothetical protein {Burkholderia
cepacia}, partial (6%)
Length = 1184
Score = 27.7 bits (60), Expect = 3.1
Identities = 17/75 (22%), Positives = 32/75 (42%)
Frame = +2
Query: 56 KTSSKSPSMATHHHHAMKKTALHSSRTGLPFKEKKEISVSARKLAATLWEINDLTPSRIK 115
+T ++ + H HA HS+++ +EK R A + R
Sbjct: 812 RTQAERTAQEKQHQHARASARAHSTKSTRSSEEKTRADARTRARARAAKD------KREA 973
Query: 116 KESMKSNKERDKVER 130
+E+ +SN++R + ER
Sbjct: 974 RETKRSNRKRRQKER 1018
>TC90573 similar to GP|15081594|gb|AAK82651.1 RSH-like protein {Capsicum
annuum}, partial (8%)
Length = 616
Score = 27.3 bits (59), Expect = 4.0
Identities = 11/34 (32%), Positives = 17/34 (49%)
Frame = -1
Query: 56 KTSSKSPSMATHHHHAMKKTALHSSRTGLPFKEK 89
KT +PS+ HHHH +K + + P + K
Sbjct: 580 KTXXSAPSIHXHHHHHXRKNSK*TEHASPPSEMK 479
>TC92439 similar to PIR|T06775|T06775 phosphoinositide-specific
phospholipase C (EC 3.1.4.-) P13 (clone SPM537) -
soybean, partial (17%)
Length = 554
Score = 26.9 bits (58), Expect = 5.2
Identities = 16/38 (42%), Positives = 22/38 (57%), Gaps = 9/38 (23%)
Frame = +1
Query: 12 KHNKIRKRGCSSTSSSSPFSR---------RYRFKRAI 40
KHN+I+ + SS+SSS P S+ R RFK A+
Sbjct: 76 KHNQIQNKTQSSSSSSPPMSKQTYSVCLCWRRRFKLAL 189
>TC85211 PIR|S22489|S22489 IgE-dependent histamine-releasing factor homolog
- alfalfa (fragment), partial (45%)
Length = 416
Score = 26.9 bits (58), Expect = 5.2
Identities = 24/101 (23%), Positives = 45/101 (43%)
Frame = -3
Query: 1 MSSCQTKHLKQKHNKIRKRGCSSTSSSSPFSRRYRFKRAILMGKKSGYNTPAPMWKTSSK 60
+SS +++QKH+K+ ++ SST+ ++ F +++ R L +
Sbjct: 345 LSSNLEHNVEQKHDKLGQKN*SSTTLNT*FPSKHKRSRETLDQQ---------------- 214
Query: 61 SPSMATHHHHAMKKTALHSSRTGLPFKEKKEISVSARKLAA 101
HH + +K L SS P K + +S+ +R L A
Sbjct: 213 ------HHPCSRRKPRLPSSCMLSPTKN*RSLSLPSRYLVA 109
>CA919642 similar to PIR|E85433|E854 SCARECROW-like protein [imported] -
Arabidopsis thaliana, partial (12%)
Length = 661
Score = 26.6 bits (57), Expect = 6.8
Identities = 16/58 (27%), Positives = 27/58 (45%)
Frame = +1
Query: 25 SSSSPFSRRYRFKRAILMGKKSGYNTPAPMWKTSSKSPSMATHHHHAMKKTALHSSRT 82
SSS F RRY L ++ + P P+ +S + + HH +K +H++ T
Sbjct: 340 SSSGSFHRRY-----YLRPEQKHLDPPCPLSPSSRRHRRIQ*LQHHRVKLQTIHNTTT 498
>BQ750912 similar to GP|7291124|gb| CG15311 gene product {Drosophila
melanogaster}, partial (2%)
Length = 682
Score = 26.6 bits (57), Expect = 6.8
Identities = 15/42 (35%), Positives = 23/42 (54%)
Frame = -2
Query: 22 SSTSSSSPFSRRYRFKRAILMGKKSGYNTPAPMWKTSSKSPS 63
SST +S+ S R R +R + +P+P +T+S SPS
Sbjct: 144 SSTPNSTSASARQRRRRRRRRAGATNNTSPSPSSRTTSYSPS 19
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.316 0.128 0.379
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 7,221,713
Number of Sequences: 36976
Number of extensions: 103875
Number of successful extensions: 871
Number of sequences better than 10.0: 52
Number of HSP's better than 10.0 without gapping: 846
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 868
length of query: 199
length of database: 9,014,727
effective HSP length: 91
effective length of query: 108
effective length of database: 5,649,911
effective search space: 610190388
effective search space used: 610190388
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 56 (26.2 bits)
Medicago: description of AC146332.8