BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146331.7 - phase: 0
(377 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC86762 similar to SP|P54967|BIOB_ARATH Biotin synthase (EC 2.8.... 760 0.0
AA660238 similar to SP|P54967|BIOB_ Biotin synthase (EC 2.8.1.6)... 46 3e-05
TC90912 GP|7291633|gb|AAF47056.1| CG12491-PA {Drosophila melanog... 30 2.0
TC88395 similar to SP|Q9LP56|WR65_ARATH Probable WRKY transcript... 28 7.6
TC83681 similar to PIR|G84772|G84772 probable serine carboxypept... 27 9.9
CB892426 similar to PIR|T05689|T0 hypothetical protein F20M13.17... 27 9.9
TC88549 similar to PIR|T03019|T03019 probable ribosomal protein ... 27 9.9
TC83398 similar to PIR|T05150|T05150 hypothetical protein F18E5.... 27 9.9
>TC86762 similar to SP|P54967|BIOB_ARATH Biotin synthase (EC 2.8.1.6)
(Biotin synthetase). [Mouse-ear cress] {Arabidopsis
thaliana}, partial (87%)
Length = 1555
Score = 760 bits (1963), Expect = 0.0
Identities = 377/377 (100%), Positives = 377/377 (100%)
Frame = +1
Query: 1 MFWLRPILRSQSRSSIWVLQHCNSFSTSSAAAIQAEKTIQNGPRNDWTKDEVKSIYDSPI 60
MFWLRPILRSQSRSSIWVLQHCNSFSTSSAAAIQAEKTIQNGPRNDWTKDEVKSIYDSPI
Sbjct: 265 MFWLRPILRSQSRSSIWVLQHCNSFSTSSAAAIQAEKTIQNGPRNDWTKDEVKSIYDSPI 444
Query: 61 LDLLFHGAQVHRHAHNFREVQQCTLLSVKTGGCSEDCSYCPQSSRYDTGLKGQKLLNKDA 120
LDLLFHGAQVHRHAHNFREVQQCTLLSVKTGGCSEDCSYCPQSSRYDTGLKGQKLLNKDA
Sbjct: 445 LDLLFHGAQVHRHAHNFREVQQCTLLSVKTGGCSEDCSYCPQSSRYDTGLKGQKLLNKDA 624
Query: 121 VLQAAVKAKEAGSTRFCMGAAWRDTIGRKTNFNQILEYVKEIKGMGMEVCCTLGMLDKDQ 180
VLQAAVKAKEAGSTRFCMGAAWRDTIGRKTNFNQILEYVKEIKGMGMEVCCTLGMLDKDQ
Sbjct: 625 VLQAAVKAKEAGSTRFCMGAAWRDTIGRKTNFNQILEYVKEIKGMGMEVCCTLGMLDKDQ 804
Query: 181 AGELKKAGLTAYNHNLDTSREYYPNIITTRTYDERLKTLEFVRDAGINVCSGGIIGLGEA 240
AGELKKAGLTAYNHNLDTSREYYPNIITTRTYDERLKTLEFVRDAGINVCSGGIIGLGEA
Sbjct: 805 AGELKKAGLTAYNHNLDTSREYYPNIITTRTYDERLKTLEFVRDAGINVCSGGIIGLGEA 984
Query: 241 EDDRVGLLHTLSTLPTHPESVPINALIAVKGTPLQDQKPVEIWEMIRMIATARITMPKAM 300
EDDRVGLLHTLSTLPTHPESVPINALIAVKGTPLQDQKPVEIWEMIRMIATARITMPKAM
Sbjct: 985 EDDRVGLLHTLSTLPTHPESVPINALIAVKGTPLQDQKPVEIWEMIRMIATARITMPKAM 1164
Query: 301 VRLSAGRVRFSVPEQALCFLAGANSIFAGEKLLTTANNDFDADQLMFKVLGLLPKAPSLD 360
VRLSAGRVRFSVPEQALCFLAGANSIFAGEKLLTTANNDFDADQLMFKVLGLLPKAPSLD
Sbjct: 1165VRLSAGRVRFSVPEQALCFLAGANSIFAGEKLLTTANNDFDADQLMFKVLGLLPKAPSLD 1344
Query: 361 DDETNEAENYKEAASSS 377
DDETNEAENYKEAASSS
Sbjct: 1345DDETNEAENYKEAASSS 1395
>AA660238 similar to SP|P54967|BIOB_ Biotin synthase (EC 2.8.1.6) (Biotin
synthetase). [Mouse-ear cress] {Arabidopsis thaliana},
partial (6%)
Length = 271
Score = 45.8 bits (107), Expect = 3e-05
Identities = 35/69 (50%), Positives = 37/69 (52%), Gaps = 1/69 (1%)
Frame = +1
Query: 48 TKDEVK-SIYDSPILDLLFHGAQVHRHAHNFREVQQCTLLSVKTGGCSEDCSYCPQSSRY 106
TKDEV+ YDSPILDLLFHG V L VK GG YCPQSSRY
Sbjct: 28 TKDEVQIPFYDSPILDLLFHGGSGS*TWLIX*GVXS-VLSLVKMGGVVRX-FYCPQSSRY 201
Query: 107 DTGLKGQKL 115
D LK + L
Sbjct: 202 DXVLKVKAL 228
>TC90912 GP|7291633|gb|AAF47056.1| CG12491-PA {Drosophila melanogaster},
partial (7%)
Length = 524
Score = 29.6 bits (65), Expect = 2.0
Identities = 15/33 (45%), Positives = 19/33 (57%)
Frame = +1
Query: 58 SPILDLLFHGAQVHRHAHNFREVQQCTLLSVKT 90
SPI LL H Q HRH H R++Q+ L + T
Sbjct: 331 SPINLLLLHPIQHHRHRHRHRQLQR*K*LRIMT 429
>TC88395 similar to SP|Q9LP56|WR65_ARATH Probable WRKY transcription factor
65 (WRKY DNA-binding protein 65). [Mouse-ear cress],
partial (43%)
Length = 1086
Score = 27.7 bits (60), Expect = 7.6
Identities = 16/73 (21%), Positives = 29/73 (38%), Gaps = 1/73 (1%)
Frame = +3
Query: 21 HCNSFSTSSAAAIQAEKTIQNGPRNDWTKDEVKSIYDSPILDLLFHGAQVHRHAHNF-RE 79
H S + S + + R W K + +++ D + + H Q+H H N R
Sbjct: 108 HLTSMAQSLPTLLHHLRKAGEPYRKGWCKSQSRNLMDQG*KERVIHHLQIHGHGENMARN 287
Query: 80 VQQCTLLSVKTGG 92
+ L+ +T G
Sbjct: 288 PSKVRLIPERTTG 326
>TC83681 similar to PIR|G84772|G84772 probable serine carboxypeptidase II
[imported] - Arabidopsis thaliana, partial (22%)
Length = 585
Score = 27.3 bits (59), Expect = 9.9
Identities = 22/83 (26%), Positives = 31/83 (36%), Gaps = 12/83 (14%)
Frame = -2
Query: 38 TIQNGPRN------------DWTKDEVKSIYDSPILDLLFHGAQVHRHAHNFREVQQCTL 85
TI NG N +W D V + S +LFH H N ++QQ +
Sbjct: 419 TISNGKNNQDHH*AIELVALNWVHDSV--VLQSTSRTMLFHSPHSQSHNLNTVQIQQFLV 246
Query: 86 LSVKTGGCSEDCSYCPQSSRYDT 108
V T S CS+ ++ T
Sbjct: 245 AVVTT*FVSLFCSFIEEAKEIPT 177
>CB892426 similar to PIR|T05689|T0 hypothetical protein F20M13.170 -
Arabidopsis thaliana, partial (15%)
Length = 580
Score = 27.3 bits (59), Expect = 9.9
Identities = 11/21 (52%), Positives = 14/21 (66%)
Frame = -3
Query: 221 FVRDAGINVCSGGIIGLGEAE 241
FVR N+CSGG++ GE E
Sbjct: 185 FVRFTISNICSGGLLNAGETE 123
>TC88549 similar to PIR|T03019|T03019 probable ribosomal protein L2
[imported] - rice mitochondrion, partial (15%)
Length = 973
Score = 27.3 bits (59), Expect = 9.9
Identities = 14/45 (31%), Positives = 23/45 (51%), Gaps = 4/45 (8%)
Frame = -2
Query: 230 CSGGIIGLGEA----EDDRVGLLHTLSTLPTHPESVPINALIAVK 270
C GG+I L + D+R+G ST+PT E + + ++ K
Sbjct: 249 CGGGLIPLSQRTRAIRDERLGSYSMRSTIPTEEEVLRLRSMTRCK 115
>TC83398 similar to PIR|T05150|T05150 hypothetical protein F18E5.40 -
Arabidopsis thaliana, partial (6%)
Length = 766
Score = 27.3 bits (59), Expect = 9.9
Identities = 11/21 (52%), Positives = 15/21 (71%)
Frame = -1
Query: 10 SQSRSSIWVLQHCNSFSTSSA 30
+Q+ S++WV CNSF SSA
Sbjct: 661 NQTTSNLWVFGFCNSFFLSSA 599
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.318 0.133 0.392
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 11,754,443
Number of Sequences: 36976
Number of extensions: 154335
Number of successful extensions: 767
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 763
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 766
length of query: 377
length of database: 9,014,727
effective HSP length: 98
effective length of query: 279
effective length of database: 5,391,079
effective search space: 1504111041
effective search space used: 1504111041
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 59 (27.3 bits)
Medicago: description of AC146331.7