BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC145767.3 - phase: 0
(807 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC82389 similar to PIR|T45912|T45912 hypothetical protein F5K20.... 143 3e-34
TC83096 weakly similar to PIR|T45912|T45912 hypothetical protein... 142 4e-34
TC83471 similar to PIR|T45912|T45912 hypothetical protein F5K20.... 123 2e-28
CA921498 similar to PIR|T45912|T45 hypothetical protein F5K20.20... 117 2e-26
AW736388 similar to GP|10178015|db Na+/H+ antiporter-like protei... 84 2e-16
AW774205 similar to PIR|T45912|T459 hypothetical protein F5K20.2... 63 4e-10
AL376050 similar to GP|10178015|dbj Na+/H+ antiporter-like prote... 35 0.11
TC87727 similar to PIR|T52392|T52392 hypothetical protein MMB12.... 30 2.8
TC82391 similar to PIR|H85354|H85354 hypothetical protein AT4g30... 29 8.2
>TC82389 similar to PIR|T45912|T45912 hypothetical protein F5K20.20 -
Arabidopsis thaliana, partial (23%)
Length = 907
Score = 143 bits (360), Expect = 3e-34
Identities = 92/268 (34%), Positives = 150/268 (55%), Gaps = 8/268 (2%)
Frame = +3
Query: 10 SASNNIWMTDDVMIKRVPLLCLQIAYNLLVSRFLFFILKPLHVPLIVAQMLTGFSVSRSL 69
++S+ W D+ M +PLL +Q + VSR L F LKPL P +VA+++ G + S
Sbjct: 108 TSSDGAWQGDNPMNHALPLLIIQTILVMFVSRTLAFFLKPLRQPRVVAEIIGGILLGPSG 287
Query: 70 LGYFEGVITLFYNPRGILAVETISNLGIMYYVFLSGLEMNSDTILRSRKKGTSMAIAGIV 129
+G + + + +E+++++G+++Y+FL GLE++ TI RS K+ ++A+AGI
Sbjct: 288 IGRNKTFMHTVFPSWSTPILESVASIGLLFYLFLVGLELDLRTINRSGKRAFNIAVAGIS 467
Query: 130 TSMLFGVGFLTLQQKLLDKKEKTH----IKAYLFWCLTLSVTGFPVLARILAKLKLLYTK 185
LF +G L QK++ +TH + ++F ++LS+T FPVLARILA+LKLL T+
Sbjct: 468 LPFLFAIGVTFLLQKVIHFNSETHKVSYFQLFIFLGVSLSITAFPVLARILAELKLLTTQ 647
Query: 186 LGKDTLTAAMLTDAYGWVLFTLLIPAANNWGE---GYYLSVITTFLFIAF-CFTVVRPIL 241
+G+ + AA D WVL L I A GE G S++ +AF V+RP++
Sbjct: 648 VGETAMAAAAFNDVAAWVLLALAIALAGG-GEHRNGVLTSILVLLSGVAFGVLFVIRPLM 824
Query: 242 TPIIENRTNKNMWRKSHMLDVFIGLFIC 269
R + R++ +LD +FIC
Sbjct: 825 -----ERISLRCSRETEVLD---EMFIC 884
>TC83096 weakly similar to PIR|T45912|T45912 hypothetical protein F5K20.20 -
Arabidopsis thaliana, partial (21%)
Length = 1227
Score = 142 bits (359), Expect = 4e-34
Identities = 110/358 (30%), Positives = 185/358 (50%), Gaps = 17/358 (4%)
Frame = +2
Query: 443 ELRVVACVHNAKHATNIIHVLEATNAT-RISPVHVSAVHLLELTRHGTAILVSQMADLNN 501
+LR++AC+H + ++I+ +E+ AT + S + + + L ELT ++IL+ + + +
Sbjct: 74 KLRILACIHGTGNIPSLINFIESVRATNKSSKIKLYVMQLTELTDSSSSILMVRSSRKSG 253
Query: 502 IAGAETTNYGPKAEFEIITTAFKEFVEQYNAVRFDTSSVVSSYTTIHEDIYNVAEEKRAS 561
G E AF+ Q V + +SS +TIHEDI ++AEEK +
Sbjct: 254 FPFINRFQKGTMQE------AFRA-CGQVGQVTVHHLTSISSLSTIHEDICHIAEEKGVA 412
Query: 562 LILLPFHKEYSTVGGSPEISHN---EHCEINENVLQHAPCSVGIFVDRGLGSLLKTKM-- 616
+I+LPFHK + G E + E+N+ VLQ APCSV + V+RG+G + ++
Sbjct: 413 MIILPFHKRWR--GEDEETIEDIGQRWREVNQRVLQSAPCSVAVLVNRGVGRRYEQRVET 586
Query: 617 ------RIITLFIGGPDDREALSIAWRMAGHSGTQLHVVRIHLLGKAA---EEKVLKKKI 667
++ +F+GGPDDR+ L + RMA H +L VVR +L + +E
Sbjct: 587 SATPGKKVCIIFVGGPDDRKVLELGSRMAEHPAIRLSVVRFNLHNEGTFRDQEHSYNTST 766
Query: 668 SKSPHGMLSTVMDGVMQKELDEEYIFSFRHKAVNNNDSIVYLEKEVHSDTGEEIPTLLNE 727
S S + M + +KELDE + F+ K + ++ Y+E + + E + +
Sbjct: 767 SASDNNMEN-------EKELDEVALNEFKTKWLG---AVEYIENDTVNIANEVL--AIGR 910
Query: 728 IDKPGYDLYIVGQGSG-KNKTIFLKLLE-WCDHPELGVIGDILASTSFGTHSSVLIVQ 783
+ + Y+L IVG+G N T + + + +H ELG IGD+L S++ G SSVL++Q
Sbjct: 911 VKE--YELVIVGKGHQLLNSTGMIDIKDSQLEHAELGPIGDLLTSSAQGITSSVLVIQ 1078
>TC83471 similar to PIR|T45912|T45912 hypothetical protein F5K20.20 -
Arabidopsis thaliana, partial (18%)
Length = 983
Score = 123 bits (309), Expect = 2e-28
Identities = 101/301 (33%), Positives = 150/301 (49%), Gaps = 23/301 (7%)
Frame = +3
Query: 529 QYNAVRFDTSSVVSSYTTIHEDIYNVAEEKRASLILLPFHKEY-STVGGSPEISHNEHCE 587
Q V +++ +SS +T+HEDI + AEEKR ++I+LPFHK + V + +E E
Sbjct: 3 QLGRVIVRSTTAISSLSTMHEDICHAAEEKRVTMIILPFHKHWRMEVDDENDKEAHEVLE 182
Query: 588 --------INENVLQHAPCSVGIFVDRG-------LGSLLKTKMRIITLFIGGPDDREAL 632
+N+ VL++APCSV + VDRG LGS + RI +F GGPDDREAL
Sbjct: 183 NAGHGWRGVNQRVLKNAPCSVAVLVDRGYGLGLKNLGSDGRVAQRICIVFFGGPDDREAL 362
Query: 633 SIAWRMAGHSGTQLHVVR-IHLLGKAAEEKVLKKKISKSPHGMLSTVMDGV-MQKE--LD 688
+ +M H + VVR + + VL++ KS S + + QKE LD
Sbjct: 363 ELGKKMVEHPAVVVTVVRFVEQNELSGNNFVLRQSPGKSTEENYSFSIAKINRQKEQVLD 542
Query: 689 EEYIFSFRHKAVNNNDSIVYLEKEVHSDTGEEIPTLLNEIDKPGYDLYIVGQGSGKNKTI 748
E + FR K +++ Y+EK +G + ++ + YDL +VG+G + +
Sbjct: 543 ENAMEEFRSKC---GETVKYIEK----GSGNVVEEVIALGESADYDLIVVGKGRFPSTMV 701
Query: 749 FLKLLEWCDHPELGVIGDILAST-SFGTHSSVLIVQQYMVGRKRVV--YKCHNEMKNSPE 805
+H ELG IGDIL S+ SSV ++QQ+ V V YK +N E
Sbjct: 702 AELAEREAEHAELGPIGDILTSSMGHKMASSVFVIQQHDVALTEDVPMYKVKVHDENVAE 881
Query: 806 V 806
V
Sbjct: 882 V 884
>CA921498 similar to PIR|T45912|T45 hypothetical protein F5K20.20 -
Arabidopsis thaliana, partial (23%)
Length = 658
Score = 117 bits (292), Expect = 2e-26
Identities = 64/186 (34%), Positives = 112/186 (59%), Gaps = 1/186 (0%)
Frame = +2
Query: 10 SASNNIWMTDDVMIKRVPLLCLQIAYNLLVSRFLFFILKPLHVPLIVAQMLTGFSVSRSL 69
++S+ IW D+ + PLL +Q L+VSR L F KPL P ++A+++ G + S
Sbjct: 92 TSSDGIWQGDNPLDYAFPLLIIQTVLVLVVSRSLAFGFKPLRQPKVIAEIIGGILLGPSA 271
Query: 70 LGYFEGVITLFYNPRGILAVETISNLGIMYYVFLSGLEMNSDTILRSRKKGTSMAIAGIV 129
LG + + + +E+++++G+++++FL GLE++ ++I RS K+ S+A GI
Sbjct: 272 LGRNTSYLHRLFPEWSMPTLESVASIGLLFFLFLVGLELDLNSIRRSGKRAFSIAACGIT 451
Query: 130 TSMLFGVGFLTLQQKLLDKKEKTHIKAYL-FWCLTLSVTGFPVLARILAKLKLLYTKLGK 188
+ G+G + +K +D +K ++ F + LS+T FPVLARILA+LKLL T++G+
Sbjct: 452 LPFVCGIGVAIVLRKTVDGADKAGFGQFIVFMGVALSITAFPVLARILAELKLLTTRVGE 631
Query: 189 DTLTAA 194
+ AA
Sbjct: 632 TAMAAA 649
>AW736388 similar to GP|10178015|db Na+/H+ antiporter-like protein
{Arabidopsis thaliana}, partial (21%)
Length = 558
Score = 84.0 bits (206), Expect = 2e-16
Identities = 59/183 (32%), Positives = 99/183 (53%), Gaps = 12/183 (6%)
Frame = +3
Query: 122 SMAIAGIVTSMLFGVGFLTLQQKLLDKKEKTHIKAYLFWC-LTLSVTGFPVLARILAKLK 180
++++ GI G+G + ++ + K T A+L + + LS+T FPVLARILA+LK
Sbjct: 18 AISMVGISLPFALGIGSSFVLKETIAKDVNT--SAFLVYMGVALSITAFPVLARILAELK 191
Query: 181 LLYTKLGKDTLTAAMLTDAYGWVLFTLLIPAANNWGEGYYLSVITTFLFIAFCFTVVRPI 240
LL T +G+ ++AA + D W+L L + + N +++ ++F+A C VV I
Sbjct: 192 LLTTSVGRMAMSAAAVNDVAAWILLALAVALSGN----SQSPLVSLWVFLAGCGFVVCSI 359
Query: 241 L--TPIIENRTNKNMWRKSHMLDVFIGLFICS---------YITDFLGTHPIVGAFVFGL 289
L PI K M ++ H + L+IC+ ++TD +G H + GAFVFG+
Sbjct: 360 LIVLPIF-----KWMAQQCHEGEPVDELYICATLASVLAAGFVTDAIGIHAMFGAFVFGI 524
Query: 290 ILP 292
++P
Sbjct: 525 LVP 533
>AW774205 similar to PIR|T45912|T459 hypothetical protein F5K20.20 -
Arabidopsis thaliana, partial (9%)
Length = 322
Score = 63.2 bits (152), Expect = 4e-10
Identities = 31/85 (36%), Positives = 49/85 (57%), Gaps = 1/85 (1%)
Frame = +1
Query: 283 GAFVFGLILPH-GKFADMVMEMSDDFVTGILCPVYFSGFGFKLDLPVLWNTPNSVLMMLI 341
GAFVFGL +P G FA+ ++E +DFV G+L P+YF+ G K D+ + L++L+
Sbjct: 1 GAFVFGLTIPKTGSFAERLIERIEDFVLGLLLPLYFASSGLKTDVTKISGGKARGLLVLV 180
Query: 342 MVLLCIPKVLSSLIVTFFFGMPARD 366
+ C K+L + +V MP +
Sbjct: 181 IATACAGKILGTFVVAMMCRMPVTE 255
>AL376050 similar to GP|10178015|dbj Na+/H+ antiporter-like protein
{Arabidopsis thaliana}, partial (3%)
Length = 521
Score = 35.0 bits (79), Expect = 0.11
Identities = 29/87 (33%), Positives = 47/87 (53%), Gaps = 1/87 (1%)
Frame = +2
Query: 703 NDSIVYLEKEVHSDTGEEIPTLLNEIDKPGYDLYIVG-QGSGKNKTIFLKLLEWCDHPEL 761
+DS++Y EK V D E + ++ +L++VG + +G+ LE + PEL
Sbjct: 2 DDSVIYEEKIV-KDAAETVASIRKF---NSCNLFLVGLRPTGE----LACALERRECPEL 157
Query: 762 GVIGDILASTSFGTHSSVLIVQQYMVG 788
G +G +L S T +SVL++QQY G
Sbjct: 158 GPVGGLLISQDCPTTASVLVMQQYHNG 238
>TC87727 similar to PIR|T52392|T52392 hypothetical protein MMB12.11
[imported] - Arabidopsis thaliana, partial (36%)
Length = 859
Score = 30.4 bits (67), Expect = 2.8
Identities = 15/46 (32%), Positives = 25/46 (53%)
Frame = +2
Query: 22 MIKRVPLLCLQIAYNLLVSRFLFFILKPLHVPLIVAQMLTGFSVSR 67
+I ++PLL I +L FF L LH+ L++ Q++ + V R
Sbjct: 419 VITKLPLLLFLIIRKILTVELRFFHLNSLHLKLVLKQLVVFWRVKR 556
>TC82391 similar to PIR|H85354|H85354 hypothetical protein AT4g30350
[imported] - Arabidopsis thaliana, partial (13%)
Length = 1065
Score = 28.9 bits (63), Expect = 8.2
Identities = 19/58 (32%), Positives = 28/58 (47%)
Frame = +3
Query: 671 PHGMLSTVMDGVMQKELDEEYIFSFRHKAVNNNDSIVYLEKEVHSDTGEEIPTLLNEI 728
P + V+ + KEL E V +N +YLEKE+ SD G +IP + E+
Sbjct: 162 PEAAIREVLKKIENKELGE---------GVFSNAHAIYLEKELPSDRG-QIPVRIKEL 305
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.325 0.140 0.416
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 25,140,246
Number of Sequences: 36976
Number of extensions: 363373
Number of successful extensions: 2498
Number of sequences better than 10.0: 18
Number of HSP's better than 10.0 without gapping: 1875
Number of HSP's successfully gapped in prelim test: 95
Number of HSP's that attempted gapping in prelim test: 584
Number of HSP's gapped (non-prelim): 2015
length of query: 807
length of database: 9,014,727
effective HSP length: 104
effective length of query: 703
effective length of database: 5,169,223
effective search space: 3633963769
effective search space used: 3633963769
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)
S2: 62 (28.5 bits)
Medicago: description of AC145767.3