BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC145513.11 - phase: 0 /pseudo
(820 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC79804 weakly similar to GP|6175165|gb|AAF04891.1| Mutator-like... 117 1e-26
BG451959 homologue to GP|6175165|gb| Mutator-like transposase {A... 96 6e-20
BI271388 similar to GP|10177186|db mutator-like transposase-like... 67 3e-11
BF646328 similar to PIR|F84533|F845 Mutator-like transposase [im... 63 5e-10
BG586356 similar to GP|9759134|dbj mutator-like transposase-like... 50 3e-06
TC78604 similar to PIR|E84456|E84456 probable acyl-CoA synthetas... 36 0.052
AL375902 similar to GP|9759134|dbj mutator-like transposase-like... 35 0.12
BG584702 weakly similar to PIR|T04811|T048 STIG1 protein homolog... 35 0.12
TC91357 similar to GP|2194137|gb|AAB61112.1| ESTs gb|R29947 gb|H... 35 0.15
BG645191 similar to GP|2194137|gb| ESTs gb|R29947 gb|H76702 come... 34 0.26
TC78881 similar to PIR|D86236|D86236 protein F14N23.5 [imported]... 34 0.26
TC80476 similar to GP|9988432|dbj|BAB12698.1 hypothetical protei... 33 0.44
TC87273 weakly similar to PIR|S66221|S66221 defensin AMP1 - Dahl... 32 0.76
BG450974 similar to PIR|T05112|T05 splicing factor 9G8-like SR p... 31 1.7
BF638163 31 1.7
TC89641 similar to GP|9988432|dbj|BAB12698.1 hypothetical protei... 31 2.2
TC85251 homologue to PIR|JQ2128|JQ2128 metallothionein - soybean... 31 2.2
TC76704 homologue to SP|Q09134|GRPA_MEDFA Abscisic acid and envi... 31 2.2
BQ137853 30 2.9
BE998238 similar to PIR|T39202|T39 heat shock protein 90 homolog... 30 2.9
>TC79804 weakly similar to GP|6175165|gb|AAF04891.1| Mutator-like
transposase {Arabidopsis thaliana}, partial (57%)
Length = 2129
Score = 117 bits (294), Expect = 1e-26
Identities = 80/281 (28%), Positives = 139/281 (48%), Gaps = 12/281 (4%)
Frame = +1
Query: 2 DEIKFLKGQVFSSSELIRTSVREFALQQRKNIYVQKSERNRVVVKC-DEGCPFYMRFSKA 60
++ F+ GQ F + R +++E A+ Q + + KS+ R KC EGCP+ +R K
Sbjct: 163 EDHNFVVGQEFPDVKAFRNAIKEAAIAQHFELRIIKSDLIRYFAKCASEGCPWRIRAVKL 342
Query: 61 PPKTYFILITYNPDHKCHYGGRTRLIG---------TTLLAKKLIPLLKLTPHMRIKKLK 111
P + F + + H C GR L G + + ++L + P K +
Sbjct: 343 PNASTFTIRSLEGTHTC---GRNALNGHHQASVDWIVSFIEERLRDNINYKP----KDIL 501
Query: 112 DACKERWGVMLSSYQLYRAKKKALELIHGAIDEQYSHLRNYAEELLRSNPGSSVLIKCKD 171
+++G+ + Q +RAK++ L I+G+ +E + L ++ EE+ ++NPGS +
Sbjct: 502 HDIHKQYGITIPYKQAWRAKERGLAAIYGSSEEGFYLLPSFCEEIKKTNPGSVAKVFTTG 681
Query: 172 SDAGPVFERIYVCFYACKRAFATTCRPLIGLDGCFLKGRDGGHLLAAIGKDGNNQMLPIA 231
+D+ F+R+++ FYA F C P++ L G LK + L+A D + + P+A
Sbjct: 682 ADSR--FQRLFISFYASIHGFVNGCLPIVALGGIQLKSKYLSTFLSATSFDADGGLFPLA 855
Query: 232 FAVVEAETKDSWD*FLELLLG--DLNGISDKKWSFISDQQK 270
FAVV+ E +SW FL L ++N + F+SD QK
Sbjct: 856 FAVVDVENDESWTWFLSELHNALEVNTECMPQIIFLSDGQK 978
Score = 38.5 bits (88), Expect = 0.011
Identities = 20/73 (27%), Positives = 31/73 (42%)
Frame = +3
Query: 588 DLPPILPPYVRRAPSRPKKQRKKGNDEKVKHSQSGAGSSSNSKSVPKRNQHSLKCGRCGE 647
D+ + PP RR P RP+K+R D R++H++ C RC +
Sbjct: 1728 DIAVVRPPKFRRPPGRPEKKRICVEDHN-------------------RDKHTVHCSRCNQ 1850
Query: 648 LGHNVRTCYGKQL 660
GH TC + +
Sbjct: 1851 TGHYKTTCKAEMI 1889
>BG451959 homologue to GP|6175165|gb| Mutator-like transposase {Arabidopsis
thaliana}, partial (28%)
Length = 658
Score = 95.9 bits (237), Expect = 6e-20
Identities = 57/174 (32%), Positives = 92/174 (52%), Gaps = 2/174 (1%)
Frame = +2
Query: 99 LKLTPHMRIKKLKDACKERWGVMLSSYQLYRAKKKALELIHGAIDEQYSHLRNYAEELLR 158
L P+ + K++ + G+ LS Q +R K+ + + G+ +E Y L Y + R
Sbjct: 56 LNENPNCKPKEILEEIHRVHGITLSYKQAWRGKEHIMAAMRGSFEEGYRLLPQYCAHVKR 235
Query: 159 SNPGSSVLIKCKDSDAGPVFERIYVCFYACKRAFATTCRPLIGLDGCFLKGRDGGHLLAA 218
+NPGS + SD F+R+++ F A CRPL+GLD +LK + G LL A
Sbjct: 236 TNPGSIASVYGNPSDN--CFQRLFISFQASIYGLLNACRPLLGLDRIYLKSKYLGTLLLA 409
Query: 219 IGKDGNNQMLPIAFAVVEAETKDSWD*FLELL--LGDLNGISDKKWSFISDQQK 270
G DG+ + P+AF VV+ E D+W FL L L ++N + + + +SD+Q+
Sbjct: 410 TGFDGDGALFPLAFGVVDEENDDNWMWFLSKLHNLLEINTENMPRLTILSDRQQ 571
>BI271388 similar to GP|10177186|db mutator-like transposase-like protein
{Arabidopsis thaliana}, partial (26%)
Length = 711
Score = 67.0 bits (162), Expect = 3e-11
Identities = 44/161 (27%), Positives = 76/161 (46%), Gaps = 8/161 (4%)
Frame = +2
Query: 10 QVFSSSELIRTSVREFALQQRKNIYVQKSERNRVVVKC-DEGCPFYMRFSKAPPKTYFIL 68
Q F+S R ++ ++++ +K++ +RV VKC +GCP+ + SK +
Sbjct: 143 QRFNSFSEFREALHKYSIAHGFAYRYKKNDSHRVTVKCKSQGCPWRIYASKLSTTQLICI 322
Query: 69 ITYNPDHKCH-------YGGRTRLIGTTLLAKKLIPLLKLTPHMRIKKLKDACKERWGVM 121
DH C Y +G + K LK +P+ R K + D K +G+
Sbjct: 323 KKMTRDHTCEGSAVKAGYRATRGWVGNIIKEK-----LKASPNYRPKDIADDIKREYGIQ 487
Query: 122 LSSYQLYRAKKKALELIHGAIDEQYSHLRNYAEELLRSNPG 162
L+ Q +RAK+ A E + G+ E Y+ L + E++ +NPG
Sbjct: 488 LNYSQAWRAKEIAREQLQGSYKEAYTQLPFFCEKIKETNPG 610
>BF646328 similar to PIR|F84533|F845 Mutator-like transposase [imported] -
Arabidopsis thaliana, partial (3%)
Length = 636
Score = 62.8 bits (151), Expect = 5e-10
Identities = 30/55 (54%), Positives = 38/55 (68%)
Frame = -3
Query: 563 RKSTFLDTYNNLILPSNGPKLWPQVDLPPILPPYVRRAPSRPKKQRKKGNDEKVK 617
RKS L TY++++LPS+GPKLWP + I P RR+ RPKK RKK NDE V+
Sbjct: 193 RKSQCLATYSHIVLPSSGPKLWPVTNTEHINPLAKRRSAGRPKKLRKKANDETVR 29
Score = 31.6 bits (70), Expect = 1.3
Identities = 22/60 (36%), Positives = 33/60 (54%)
Frame = -2
Query: 450 RRKLRAGLQIGVEIGTMLDLLFIMALTHML*ILEKIHVPAGNGICLAYLVHMQLLAYGII 509
+R G Q+G+ I + L++ M L ++L*I K V +G L Y VHM A+GI+
Sbjct: 494 KRAAGGGRQLGMVIWK*IILMYQMRLINIL*IWLKGLVLVVSGT*LVYHVHMLFPAFGIM 315
>BG586356 similar to GP|9759134|dbj mutator-like transposase-like protein
{Arabidopsis thaliana}, partial (20%)
Length = 696
Score = 50.1 bits (118), Expect = 3e-06
Identities = 30/119 (25%), Positives = 60/119 (50%), Gaps = 4/119 (3%)
Frame = +3
Query: 9 GQVFSSSELIRTSVREFALQQRKNIYVQKSERNRVVVKCD-EGCPFYMRFSKAPPKTYFI 67
GQ F E R ++++ A+ ++ + KS+R+R + KC EGCP+ + +K P F
Sbjct: 336 GQEFPDVETCRRTLKDIAIAMHFDLRIVKSDRSRFIAKCSKEGCPWRVHVAKCPGVPTFT 515
Query: 68 LITYNPDHKCHYGGRT---RLIGTTLLAKKLIPLLKLTPHMRIKKLKDACKERWGVMLS 123
+ T DH C G R + +A+ + ++ P ++ +++ +++ GV +S
Sbjct: 516 IRTLQADHTCE-GVRNLHHQQASVGWVARSVESRIRDNPQVKPREILQDIRDQHGVAVS 689
>TC78604 similar to PIR|E84456|E84456 probable acyl-CoA synthetase [imported]
- Arabidopsis thaliana, partial (91%)
Length = 2573
Score = 36.2 bits (82), Expect = 0.052
Identities = 24/83 (28%), Positives = 34/83 (40%), Gaps = 12/83 (14%)
Frame = -2
Query: 690 YLTCIMHIPNQSNCSATSSTTCTQEERAS*QERTKQKGW--------EIIWNHTHN-CTN 740
+L I I + T+ TC+Q R +T +G IWNH+HN C
Sbjct: 1180 FLDFIRCISQSHRTAVTNCNTCSQHNRLCCHFKTMSEGQISQVHVLATQIWNHSHNSCCG 1001
Query: 741 SFN---HNRSITWKHNCTTSCKH 760
+N N W+ CT +C H
Sbjct: 1000 GYNVPVSNHDTLWQ-TCTATCIH 935
>AL375902 similar to GP|9759134|dbj mutator-like transposase-like protein
{Arabidopsis thaliana}, partial (20%)
Length = 542
Score = 35.0 bits (79), Expect = 0.12
Identities = 22/64 (34%), Positives = 30/64 (46%)
Frame = +2
Query: 592 ILPPYVRRAPSRPKKQRKKGNDEKVKHSQSGAGSSSNSKSVPKRNQHSLKCGRCGELGHN 651
I PP +RR P RPKK KV ++ KR + ++CGRC LGH+
Sbjct: 239 IHPPKIRRPPGRPKK--------KVLRVENF-----------KRPKRVVQCGRCHMLGHS 361
Query: 652 VRTC 655
+ C
Sbjct: 362 QKKC 373
>BG584702 weakly similar to PIR|T04811|T048 STIG1 protein homolog F10M23.220
- Arabidopsis thaliana, partial (43%)
Length = 719
Score = 35.0 bits (79), Expect = 0.12
Identities = 17/47 (36%), Positives = 20/47 (42%), Gaps = 4/47 (8%)
Frame = +2
Query: 772 TCCNQHCATC---KQQCANCKQHCATCKQLCAN-CEATNSGGKHTHG 814
TCCN C K C CK+HC + C C TN +H G
Sbjct: 338 TCCNNKCIDLGYDKHNCGACKKHCKYTQTCCRGACVDTNYDKRHCGG 478
>TC91357 similar to GP|2194137|gb|AAB61112.1| ESTs gb|R29947 gb|H76702 come
from this gene. {Arabidopsis thaliana}, partial (40%)
Length = 766
Score = 34.7 bits (78), Expect = 0.15
Identities = 28/117 (23%), Positives = 42/117 (34%)
Frame = +3
Query: 634 KRNQHSLKCGRCGELGHNVRTCYGKQLGDRMLAPGENQVQTVMCFTVMC*ICNCHMYLTC 693
K+ S C +CG GHN RTC + +++M F V N H+ T
Sbjct: 204 KKMSLSRTCSQCGNNGHNSRTC-----------NDGGEEKSIMIFGVRLTGGNNHLNTTT 350
Query: 694 IMHIPNQSNCSATSSTTCTQEERAS*QERTKQKGWEIIWNHTHNCTNSFNHNRSITW 750
N S + S T +Q E+ Q+ + + H S R + W
Sbjct: 351 TNTTINNSFRKSASMTNLSQYEQPPPQDSNPAHAGYVSHDIVHASGRSRERKRGVPW 521
>BG645191 similar to GP|2194137|gb| ESTs gb|R29947 gb|H76702 come from this
gene. {Arabidopsis thaliana}, partial (40%)
Length = 649
Score = 33.9 bits (76), Expect = 0.26
Identities = 28/117 (23%), Positives = 42/117 (34%)
Frame = +1
Query: 634 KRNQHSLKCGRCGELGHNVRTCYGKQLGDRMLAPGENQVQTVMCFTVMC*ICNCHMYLTC 693
K+ S C +CG GHN RTC + +++M F V N H+ T
Sbjct: 97 KKMSLSRTCSQCGNNGHNSRTC-----------NDGGEEKSIMIFGVRLTGGNNHLNTTT 243
Query: 694 IMHIPNQSNCSATSSTTCTQEERAS*QERTKQKGWEIIWNHTHNCTNSFNHNRSITW 750
N S + S T +Q E+ Q+ + + H S R + W
Sbjct: 244 TNTTINNSFRKSASMTNLSQYEQPPPQDSNPADAGYVSDDIVHASGRSRERKRGVPW 414
>TC78881 similar to PIR|D86236|D86236 protein F14N23.5 [imported] -
Arabidopsis thaliana, partial (31%)
Length = 1710
Score = 33.9 bits (76), Expect = 0.26
Identities = 42/163 (25%), Positives = 68/163 (40%), Gaps = 19/163 (11%)
Frame = +3
Query: 638 HSLKCGRCGELGHNVRTCY-GK-QLGDRMLA-----PGENQV--QTVMCFTVMC*I-CNC 687
H C C L NV+TC+ GK +LG+ L+ P +QV +T+ C C + C+
Sbjct: 327 HPGSCVECPLLPTNVKTCFCGKTRLGEERLSCLDPIPTCSQVCGKTLPCGMHHCTLTCHD 506
Query: 688 HMYLTCIMHIPNQSNCSATSSTTCTQEERAS*QERTKQKGWEIIWN-HTHNCT------- 739
C + + + C +TS T ++ + ++ T +K N H C+
Sbjct: 507 GDCSPCSVLVSQKCRCGSTSKTVECCKKTMANEKFTCEKPCGKKRNCGRHRCSERCCPLS 686
Query: 740 NSFNH-NRSITWKHNCTTSCKHHFTTT*HHCTTTCCNQHCATC 781
N N N + + H C+ C H C + C + HC C
Sbjct: 687 NPNNILNATDSDPHLCSIPCGKKLRCGKHACDSNCHSGHCPPC 815
Score = 32.7 bits (73), Expect = 0.58
Identities = 16/54 (29%), Positives = 23/54 (41%), Gaps = 6/54 (11%)
Frame = +3
Query: 754 CTTSCKHHFTTT*HHCTTTCCNQHCATC------KQQCANCKQHCATCKQLCAN 801
C+ C HHCT TC + C+ C K +C + + CK+ AN
Sbjct: 441 CSQVCGKTLPCGMHHCTLTCHDGDCSPCSVLVSQKCRCGSTSKTVECCKKTMAN 602
>TC80476 similar to GP|9988432|dbj|BAB12698.1 hypothetical protein {Oryza
sativa (japonica cultivar-group)}, partial (34%)
Length = 778
Score = 33.1 bits (74), Expect = 0.44
Identities = 12/21 (57%), Positives = 14/21 (66%)
Frame = +1
Query: 641 KCGRCGELGHNVRTCYGKQLG 661
KC CG++GHN RTC LG
Sbjct: 46 KCSHCGKIGHNCRTCTSCTLG 108
>TC87273 weakly similar to PIR|S66221|S66221 defensin AMP1 - Dahlia merckii,
partial (70%)
Length = 735
Score = 32.3 bits (72), Expect = 0.76
Identities = 25/91 (27%), Positives = 31/91 (33%), Gaps = 22/91 (24%)
Frame = +3
Query: 746 RSITWKHNC--TTSCKHHFTTT*HHCTTTC----------CNQHCATCKQQCANCKQHCA 793
RS TW C T +CK H C C + CA K + C++
Sbjct: 126 RSTTWSGPCLNTGNCKRQCINVEHATFGACHRQGFGFACFCYKKCAPKKVEPKLCERRSK 305
Query: 794 T----------CKQLCANCEATNSGGKHTHG 814
T CK+ C N E SG H G
Sbjct: 306 TWSGPCLISGNCKRQCINVEHATSGACHRQG 398
>BG450974 similar to PIR|T05112|T05 splicing factor 9G8-like SR protein
RSZp22 [validated] - Arabidopsis thaliana, partial (54%)
Length = 364
Score = 31.2 bits (69), Expect = 1.7
Identities = 15/47 (31%), Positives = 18/47 (37%)
Frame = +1
Query: 609 KKGNDEKVKHSQSGAGSSSNSKSVPKRNQHSLKCGRCGELGHNVRTC 655
K G ++ H+ G R LKC CGE GH R C
Sbjct: 220 KNGWRVQLSHNSKSGGGGGRGGGRGGRGGDDLKCYECGEPGHFAREC 360
>BF638163
Length = 687
Score = 31.2 bits (69), Expect = 1.7
Identities = 10/22 (45%), Positives = 16/22 (72%)
Frame = +2
Query: 634 KRNQHSLKCGRCGELGHNVRTC 655
K+ + +CGRCG +GH+ R+C
Sbjct: 383 KKTYNDTQCGRCGLMGHSSRSC 448
>TC89641 similar to GP|9988432|dbj|BAB12698.1 hypothetical protein {Oryza
sativa (japonica cultivar-group)}, partial (44%)
Length = 1080
Score = 30.8 bits (68), Expect = 2.2
Identities = 10/15 (66%), Positives = 11/15 (72%)
Frame = +3
Query: 641 KCGRCGELGHNVRTC 655
KC CG +GHN RTC
Sbjct: 93 KCSHCGNIGHNSRTC 137
>TC85251 homologue to PIR|JQ2128|JQ2128 metallothionein - soybean, partial
(93%)
Length = 1554
Score = 30.8 bits (68), Expect = 2.2
Identities = 25/98 (25%), Positives = 33/98 (33%), Gaps = 4/98 (4%)
Frame = -1
Query: 687 CHMYLT----CIMHIPNQSNCSATSSTTCTQEERAS*QERTKQKGWEIIWNHTHNCTNSF 742
C Y+T C P S T TC A+ T + N T CT+
Sbjct: 717 CSSYITSSKNCTSFKPKSPTTSTTKKFTCLNSNIATTVATTTKDITNTSPNTTSPCTSKG 538
Query: 743 NHNRSITWKHNCTTSCKHHFTTT*HHCTTTCCNQHCAT 780
N S + SC + TT + T CN+ T
Sbjct: 537 NTGASTCTRKASANSCTCYLTT---NTRTNACNRSTCT 433
>TC76704 homologue to SP|Q09134|GRPA_MEDFA Abscisic acid and environmental
stress inducible protein. [Sickle medic] {Medicago
falcata}, partial (81%)
Length = 771
Score = 30.8 bits (68), Expect = 2.2
Identities = 22/86 (25%), Positives = 29/86 (33%), Gaps = 3/86 (3%)
Frame = -2
Query: 731 IWNHTHNCTNSFNHNRSITWKHNCTT---SCKHHFTTT*HHCTTTCCNQHCATCKQQCAN 787
I NH H N +H S H+C C HH HH C + HC + N
Sbjct: 446 IGNHLHRGCNRLHHGCS---HHHCNRLHHGCSHHRCNHLHH---GCIHHHCCIHHHRGCN 285
Query: 788 CKQHCATCKQLCANCEATNSGGKHTH 813
C + C + G + H
Sbjct: 284 LHHCCIHPHRGCNLLHCNHHRGCNLH 207
>BQ137853
Length = 978
Score = 30.4 bits (67), Expect = 2.9
Identities = 14/46 (30%), Positives = 20/46 (43%)
Frame = +2
Query: 768 HCTTTCCNQHCATCKQQCANCKQHCATCKQLCANCEATNSGGKHTH 813
H + C +H T + +CA + HC C L A G+H H
Sbjct: 323 HIVSACTTRHRVTPRAECAVRQSHCYACYALYARMLC----GRHVH 448
>BE998238 similar to PIR|T39202|T39 heat shock protein 90 homolog - fission
yeast (Schizosaccharomyces pombe), partial (30%)
Length = 654
Score = 30.4 bits (67), Expect = 2.9
Identities = 13/31 (41%), Positives = 17/31 (53%)
Frame = -1
Query: 581 PKLWPQVDLPPILPPYVRRAPSRPKKQRKKG 611
P+ W LPP PP+ R PS+P R+ G
Sbjct: 177 PRSWASRRLPPP*PPHRHRPPSQPPWSRRAG 85
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.344 0.151 0.515
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 31,831,141
Number of Sequences: 36976
Number of extensions: 583447
Number of successful extensions: 7185
Number of sequences better than 10.0: 61
Number of HSP's better than 10.0 without gapping: 3039
Number of HSP's successfully gapped in prelim test: 472
Number of HSP's that attempted gapping in prelim test: 3754
Number of HSP's gapped (non-prelim): 4039
length of query: 820
length of database: 9,014,727
effective HSP length: 104
effective length of query: 716
effective length of database: 5,169,223
effective search space: 3701163668
effective search space used: 3701163668
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 38 (21.6 bits)
S2: 62 (28.5 bits)
Medicago: description of AC145513.11