BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC145372.5 - phase: 0
(369 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC83019 similar to GP|4049632|gb|AAC97601.1| pyruvate dehydrogen... 385 0.0
TC93120 similar to GP|4049632|gb|AAC97601.1| pyruvate dehydrogen... 236 3e-76
TC89818 similar to GP|1448917|gb|AAB04607.1| threonine synthase ... 31 0.87
TC86951 homologue to GP|2745902|gb|AAB94773.1| ERS-like ethylene... 31 0.87
BG644459 weakly similar to PIR|D86356|D86 hypothetical protein A... 30 1.5
BI266143 similar to GP|13537196|d histidine kinase {Arabidopsis ... 29 3.3
TC92708 similar to GP|13877549|gb|AAK43852.1 Unknown protein {Ar... 28 4.3
TC80132 similar to PIR|D96538|D96538 cytosolic tRNA-Ala syntheta... 28 5.6
TC80227 similar to GP|13537198|dbj|BAB40775. histidine kinase {A... 28 5.6
AJ498479 28 7.3
TC83618 28 7.3
AW686764 similar to GP|13537198|d histidine kinase {Arabidopsis ... 27 9.6
>TC83019 similar to GP|4049632|gb|AAC97601.1| pyruvate dehydrogenase kinase
{Arabidopsis thaliana}, partial (89%)
Length = 1140
Score = 385 bits (988), Expect(2) = 0.0
Identities = 192/194 (98%), Positives = 192/194 (98%)
Frame = +3
Query: 24 FKQTGVSLRYMMDFGSKPTSKNLLISAQFLHKELAIRIARRAVELERLPCDLSQKPAVLK 83
FKQTGVSLRYMMDFGSKPTSKNLLISAQFLHKELAIRIARRAVELERLPCDLSQKPAVLK
Sbjct: 3 FKQTGVSLRYMMDFGSKPTSKNLLISAQFLHKELAIRIARRAVELERLPCDLSQKPAVLK 182
Query: 84 VRDWYLDSFRDIRSFSEIKNMNDEKEFTEVIKAIKVRHNNVVPTMALGVQQLKKDVDPKI 143
VRDWYLDSFRDIRSFSEIKNMNDEKEFTEVIKAIKVRHNNVVPTMALGVQQLKKDVDPKI
Sbjct: 183 VRDWYLDSFRDIRSFSEIKNMNDEKEFTEVIKAIKVRHNNVVPTMALGVQQLKKDVDPKI 362
Query: 144 INQDFVEIHQFLDRFYMSRIGIRMLIGQHVELHSPNPPPHVVGYIHTRMSPVEVARNAVD 203
INQDFVEIHQFLDRFYMSRIGIRMLIGQHVELHSPNPPPHVV YIHTRMSPVEVARNAVD
Sbjct: 363 INQDFVEIHQFLDRFYMSRIGIRMLIGQHVELHSPNPPPHVVSYIHTRMSPVEVARNAVD 542
Query: 204 DARSICSRQYGSAP 217
DA SICSRQYGSAP
Sbjct: 543 DAPSICSRQYGSAP 584
Score = 315 bits (808), Expect(2) = 0.0
Identities = 152/153 (99%), Positives = 153/153 (99%)
Frame = +1
Query: 217 PDVHIYGDPDFTFPYVPAHLHLMVFELVKNSLRAVEERYMNSDKDPPPIRIIVADGLEDV 276
PDVHIYGDP+FTFPYVPAHLHLMVFELVKNSLRAVEERYMNSDKDPPPIRIIVADGLEDV
Sbjct: 583 PDVHIYGDPEFTFPYVPAHLHLMVFELVKNSLRAVEERYMNSDKDPPPIRIIVADGLEDV 762
Query: 277 TIKISDEGGGIPRSGLRKIFTYLYSTARNPLDEHADLGVGDNVTMAGYGYGLPISRLYAR 336
TIKISDEGGGIPRSGLRKIFTYLYSTARNPLDEHADLGVGDNVTMAGYGYGLPISRLYAR
Sbjct: 763 TIKISDEGGGIPRSGLRKIFTYLYSTARNPLDEHADLGVGDNVTMAGYGYGLPISRLYAR 942
Query: 337 YFGGDLQIISMENYGTDAYLHLSRLGDSQEPLP 369
YFGGDLQIISMENYGTDAYLHLSRLGDSQEPLP
Sbjct: 943 YFGGDLQIISMENYGTDAYLHLSRLGDSQEPLP 1041
>TC93120 similar to GP|4049632|gb|AAC97601.1| pyruvate dehydrogenase kinase
{Arabidopsis thaliana}, partial (45%)
Length = 635
Score = 236 bits (602), Expect(2) = 3e-76
Identities = 118/155 (76%), Positives = 136/155 (87%), Gaps = 1/155 (0%)
Frame = +3
Query: 6 AIETFSKSLIEEVHKWGCFKQTGVSLRYMMDFGSKPTSKNLLISAQFLHKELAIRIARRA 65
AIE FSKSL++EVHKWGC KQTGVSLRYMM+FGS+PT KNLLISAQFL KELAIRIARRA
Sbjct: 78 AIEAFSKSLLQEVHKWGCLKQTGVSLRYMMEFGSQPTDKNLLISAQFLQKELAIRIARRA 257
Query: 66 VELERLPCDLSQKPAVLKVRDWYLDSFRDIRSFSEIKNMNDEKEFTEVIKAIKVRHNNVV 125
+ELE LP LS+KPA+LKVRDWY+DSFRDIRS E+K+M DE+EFT+VIKAIKVRHNNVV
Sbjct: 258 IELESLPYGLSKKPAILKVRDWYVDSFRDIRSCPEVKDMKDEREFTDVIKAIKVRHNNVV 437
Query: 126 PTMALGVQQLKKDVDPK-IINQDFVEIHQFLDRFY 159
PTMALGVQQLKK+ + K +I + + +FLDRFY
Sbjct: 438 PTMALGVQQLKKEFENKRLIVKILLRFMKFLDRFY 542
Score = 67.0 bits (162), Expect(2) = 3e-76
Identities = 28/32 (87%), Positives = 31/32 (96%)
Frame = +1
Query: 158 FYMSRIGIRMLIGQHVELHSPNPPPHVVGYIH 189
F +SRIG+RMLIGQHVELH+PNPPPHVVGYIH
Sbjct: 538 FTLSRIGVRMLIGQHVELHNPNPPPHVVGYIH 633
>TC89818 similar to GP|1448917|gb|AAB04607.1| threonine synthase
{Arabidopsis thaliana}, partial (32%)
Length = 684
Score = 30.8 bits (68), Expect = 0.87
Identities = 11/30 (36%), Positives = 18/30 (59%)
Frame = +1
Query: 172 HVELHSPNPPPHVVGYIHTRMSPVEVARNA 201
H+ HSP+PPP +H R+SP ++ +
Sbjct: 55 HITAHSPSPPPPPTTTLHHRISPPTTSKTS 144
>TC86951 homologue to GP|2745902|gb|AAB94773.1| ERS-like ethylene receptor
{Pisum sativum}, complete
Length = 2274
Score = 30.8 bits (68), Expect = 0.87
Identities = 32/142 (22%), Positives = 55/142 (38%), Gaps = 18/142 (12%)
Frame = +3
Query: 230 PYVPAH-------LHLMVFELVKNSLRAVEERYMN-----------SDKDPPPIRIIVAD 271
P +P H L + +V N+++ +E Y++ D PP +D
Sbjct: 1494 PDLPTHAIGDEKRLMQTLLNVVGNAVKYTKEGYVSIRASVAKPESLQDWRPPEFYPASSD 1673
Query: 272 GLEDVTIKISDEGGGIPRSGLRKIFTYLYSTARNPLDEHADLGVGDNVTMAGYGYGLPIS 331
G + +++ D G GI + +FT + P+ + +G G GL I
Sbjct: 1674 GHFYIRVQVKDSGCGILPQDIPHLFTKFTQSQSGPVR-----------SSSGSGLGLAIC 1820
Query: 332 RLYARYFGGDLQIISMENYGTD 353
+ + GG I ME+ G D
Sbjct: 1821 KRFVNLMGGH---IWMESEGHD 1877
>BG644459 weakly similar to PIR|D86356|D86 hypothetical protein AAF87258.1
[imported] - Arabidopsis thaliana, partial (25%)
Length = 776
Score = 30.0 bits (66), Expect = 1.5
Identities = 13/38 (34%), Positives = 27/38 (70%), Gaps = 1/38 (2%)
Frame = +3
Query: 247 SLRAVEERYMNSD-KDPPPIRIIVADGLEDVTIKISDE 283
S R + ++++S+ +D PP+ IV+DG+ + T+K++ E
Sbjct: 288 SFRNLIAKFVSSENEDVPPVTCIVSDGVMNFTLKVAQE 401
>BI266143 similar to GP|13537196|d histidine kinase {Arabidopsis thaliana},
partial (9%)
Length = 470
Score = 28.9 bits (63), Expect = 3.3
Identities = 22/79 (27%), Positives = 31/79 (38%), Gaps = 4/79 (5%)
Frame = +2
Query: 278 IKISDEGGGIPRSGLRKIFTYLY----STARNPLDEHADLGVGDNVTMAGYGYGLPISRL 333
+ + D G GIP +IFT ST+R T G G GL IS+
Sbjct: 86 VTVEDTGIGIPTDAQSRIFTPFMQADSSTSR---------------TYGGTGIGLSISKC 220
Query: 334 YARYFGGDLQIISMENYGT 352
GG++ +S G+
Sbjct: 221 LVDLMGGEIGFVSEPEIGS 277
>TC92708 similar to GP|13877549|gb|AAK43852.1 Unknown protein {Arabidopsis
thaliana}, partial (66%)
Length = 722
Score = 28.5 bits (62), Expect = 4.3
Identities = 16/51 (31%), Positives = 23/51 (44%)
Frame = +2
Query: 171 QHVELHSPNPPPHVVGYIHTRMSPVEVARNAVDDARSICSRQYGSAPDVHI 221
Q+ +LH+P+PP H + SP N +D I S S VH+
Sbjct: 173 QNFQLHTPSPPLH-QSMDESEQSPKNYYPNQRNDITRISSMSSSSTTSVHV 322
>TC80132 similar to PIR|D96538|D96538 cytosolic tRNA-Ala synthetase
[imported] - Arabidopsis thaliana, partial (25%)
Length = 908
Score = 28.1 bits (61), Expect = 5.6
Identities = 13/32 (40%), Positives = 18/32 (55%), Gaps = 1/32 (3%)
Frame = +1
Query: 326 YGLPISRLYARYFGGDLQI-ISMENYGTDAYL 356
Y LP R+Y YFGGD + + +N D +L
Sbjct: 529 YNLPSDRIYVTYFGGDEKTGLGPDNEARDIWL 624
>TC80227 similar to GP|13537198|dbj|BAB40775. histidine kinase {Arabidopsis
thaliana}, partial (22%)
Length = 1693
Score = 28.1 bits (61), Expect = 5.6
Identities = 13/48 (27%), Positives = 23/48 (47%)
Frame = +3
Query: 273 LEDVTIKISDEGGGIPRSGLRKIFTYLYSTARNPLDEHADLGVGDNVT 320
L ++ + + D G GIPR IFT + +H G+G +++
Sbjct: 6 LINLIVSVEDTGDGIPRESQPLIFTPFMQVGSSISRKHGGTGIGLSIS 149
>AJ498479
Length = 202
Score = 27.7 bits (60), Expect = 7.3
Identities = 17/38 (44%), Positives = 23/38 (59%), Gaps = 3/38 (7%)
Frame = +2
Query: 18 VHKWGCFKQTGVSLR---YMMDFGSKPTSKNLLISAQF 52
VHK GC + LR ++ + GSK S+ L+ISAQF
Sbjct: 71 VHKSGCRGYSAQ*LRHGCFLSEEGSKVPSRLLIISAQF 184
>TC83618
Length = 807
Score = 27.7 bits (60), Expect = 7.3
Identities = 14/31 (45%), Positives = 18/31 (57%), Gaps = 5/31 (16%)
Frame = +2
Query: 222 YGDPDFTFP-----YVPAHLHLMVFELVKNS 247
Y +P F FP YVPAHL L +F + +S
Sbjct: 449 YSNPHFVFPHSHWLYVPAHLKLNLFAALISS 541
>AW686764 similar to GP|13537198|d histidine kinase {Arabidopsis thaliana},
partial (12%)
Length = 652
Score = 27.3 bits (59), Expect = 9.6
Identities = 19/83 (22%), Positives = 31/83 (36%)
Frame = +2
Query: 270 ADGLEDVTIKISDEGGGIPRSGLRKIFTYLYSTARNPLDEHADLGVGDNVTMAGYGYGLP 329
++ L + + + D G GIP IFT + +H G G GL
Sbjct: 302 SNDLISLIVSVEDTGDGIPLESQPMIFTPFMQVGPSISRKH-----------GGTGIGLS 448
Query: 330 ISRLYARYFGGDLQIISMENYGT 352
IS+ G++ +S G+
Sbjct: 449 ISKCLVGLMNGEIGFVSEPKIGS 517
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.321 0.140 0.412
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 10,301,548
Number of Sequences: 36976
Number of extensions: 136790
Number of successful extensions: 666
Number of sequences better than 10.0: 24
Number of HSP's better than 10.0 without gapping: 661
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 666
length of query: 369
length of database: 9,014,727
effective HSP length: 98
effective length of query: 271
effective length of database: 5,391,079
effective search space: 1460982409
effective search space used: 1460982409
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 59 (27.3 bits)
Medicago: description of AC145372.5