BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC145329.3 + phase: 0 /partial
(121 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC87441 similar to GP|21928031|gb|AAM78044.1 At3g62600/F26K9_30 ... 243 1e-65
TC78759 similar to GP|21618097|gb|AAM67147.1 putative heat shock... 84 2e-17
TC85119 similar to PIR|G84590|G84590 probable heat shock protein... 79 4e-16
TC90074 similar to PIR|G84590|G84590 probable heat shock protein... 74 2e-14
TC91526 similar to PIR|G84590|G84590 probable heat shock protein... 72 4e-14
TC77626 homologue to GP|5802244|gb|AAD51625.1| seed maturation p... 70 3e-13
TC86029 homologue to GP|5802244|gb|AAD51625.1| seed maturation p... 69 6e-13
TC85313 PIR|T09338|T09338 DnaJ-like protein MsJ1 - alfalfa, comp... 67 2e-12
BG644408 homologue to PIR|T07371|T07 dnaJ protein homolog - pota... 64 1e-11
TC87379 similar to PIR|T04618|T04618 heat shock protein homolog ... 50 2e-07
TC78760 similar to PIR|T48161|T48161 heat shock protein 40-like ... 46 3e-06
TC81458 similar to GP|21553367|gb|AAM62460.1 DnaJ protein-like {... 44 1e-05
TC77858 similar to GP|20197886|gb|AAD22362.2 putative DnaJ prote... 42 7e-05
TC90708 weakly similar to PIR|S26703|S26703 dnaJ protein homolog... 27 1.9
BQ150974 27 2.4
BE321051 homologue to GP|15293149|gb| putative HSP protein {Arab... 26 3.2
TC80077 similar to GP|16604607|gb|AAL24096.1 putative AtBgamma p... 26 4.2
BG454163 26 4.2
TC76825 homologue to SP|O81361|RS8_PRUAR 40S ribosomal protein S... 26 4.2
TC78420 similar to GP|20453070|gb|AAM19780.1 AT5g66920/MUD21_18 ... 25 5.5
>TC87441 similar to GP|21928031|gb|AAM78044.1 At3g62600/F26K9_30
{Arabidopsis thaliana}, partial (94%)
Length = 1563
Score = 243 bits (621), Expect = 1e-65
Identities = 121/121 (100%), Positives = 121/121 (100%)
Frame = +3
Query: 1 EVLFYEDGEPIIDGESGDLRFRIRTAPHELFKREGNDLHTTVTITLVQALVGFEKTIKHL 60
EVLFYEDGEPIIDGESGDLRFRIRTAPHELFKREGNDLHTTVTITLVQALVGFEKTIKHL
Sbjct: 855 EVLFYEDGEPIIDGESGDLRFRIRTAPHELFKREGNDLHTTVTITLVQALVGFEKTIKHL 1034
Query: 61 DEHLVDISSKGITNPKQVRKFKGEGMPLHTSTKKGDLYVTFEVLFPTTLSEEQKTKIKSI 120
DEHLVDISSKGITNPKQVRKFKGEGMPLHTSTKKGDLYVTFEVLFPTTLSEEQKTKIKSI
Sbjct: 1035DEHLVDISSKGITNPKQVRKFKGEGMPLHTSTKKGDLYVTFEVLFPTTLSEEQKTKIKSI 1214
Query: 121 L 121
L
Sbjct: 1215L 1217
>TC78759 similar to GP|21618097|gb|AAM67147.1 putative heat shock protein
{Arabidopsis thaliana}, partial (89%)
Length = 1344
Score = 83.6 bits (205), Expect = 2e-17
Identities = 48/105 (45%), Positives = 62/105 (58%), Gaps = 1/105 (0%)
Frame = +1
Query: 18 DLRFRIRTAPHELFKREGNDLHTTVTITLVQALVGFEKTIKHLDEHLVDISSKGITNPKQ 77
DL F I PH +FKR+GNDL T I+LV+AL G+ I LD + + I +P
Sbjct: 769 DLVFIIDEKPHTVFKRDGNDLVVTQKISLVEALTGYTAQITTLDGRNLTVPVNTIISPSY 948
Query: 78 VRKFKGEGMPL-HTSTKKGDLYVTFEVLFPTTLSEEQKTKIKSIL 121
KGEGMP+ +KKG+L V F V FP+ L+ EQKT IK +L
Sbjct: 949 EEVIKGEGMPIPKEPSKKGNLRVKFNVKFPSRLTSEQKTGIKRLL 1083
>TC85119 similar to PIR|G84590|G84590 probable heat shock protein [imported]
- Arabidopsis thaliana, partial (72%)
Length = 796
Score = 79.0 bits (193), Expect = 4e-16
Identities = 45/122 (36%), Positives = 65/122 (52%), Gaps = 1/122 (0%)
Frame = +3
Query: 1 EVLFYEDGEPIIDGESGDLRFRIRTAPHELFKREGNDLHTTVTITLVQALVGFEKTIKHL 60
++ F E G + + DL F I PH +F REGNDL T I+L +AL G+ + L
Sbjct: 399 KITFPEKGNEQPNVTAADLIFVIDERPHSVFSREGNDLIVTQKISLAEALTGYTVHLTTL 578
Query: 61 DEHLVDISSKGITNPKQVRKFKGEGMPL-HTSTKKGDLYVTFEVLFPTTLSEEQKTKIKS 119
D + I + +P EGMPL TKKG+L + F + FPT L++EQK ++
Sbjct: 579 DGRNLSIPINNVIHPNYEEVVPKEGMPLPKDPTKKGNLRIKFNIKFPTRLTDEQKAGVRK 758
Query: 120 IL 121
+L
Sbjct: 759 LL 764
>TC90074 similar to PIR|G84590|G84590 probable heat shock protein [imported]
- Arabidopsis thaliana, partial (88%)
Length = 1287
Score = 73.6 bits (179), Expect = 2e-14
Identities = 42/122 (34%), Positives = 64/122 (52%), Gaps = 1/122 (0%)
Frame = +1
Query: 1 EVLFYEDGEPIIDGESGDLRFRIRTAPHELFKREGNDLHTTVTITLVQALVGFEKTIKHL 60
++ F E G + DL F I PH +F R+GNDL T I+L +AL G+ + L
Sbjct: 649 KITFPEKGNEQPNVIPADLIFVIDEKPHGVFTRDGNDLVATQKISLAEALTGYTVRLTTL 828
Query: 61 DEHLVDISSKGITNPKQVRKFKGEGMPL-HTSTKKGDLYVTFEVLFPTTLSEEQKTKIKS 119
D ++++ + +P EGMP+ +KKG+L + F + FP L+ EQK IK
Sbjct: 829 DGRVLNVPINNVIHPSYEEVIPKEGMPIPKDPSKKGNLRIKFNIKFPARLTSEQKIGIKK 1008
Query: 120 IL 121
+L
Sbjct: 1009LL 1014
>TC91526 similar to PIR|G84590|G84590 probable heat shock protein [imported]
- Arabidopsis thaliana, partial (37%)
Length = 743
Score = 72.4 bits (176), Expect = 4e-14
Identities = 40/105 (38%), Positives = 57/105 (54%), Gaps = 1/105 (0%)
Frame = +3
Query: 18 DLRFRIRTAPHELFKREGNDLHTTVTITLVQALVGFEKTIKHLDEHLVDISSKGITNPKQ 77
D+ F I PH +F REGNDL T I+L +AL G + LD + + + +P+
Sbjct: 63 DIIFVIDEKPHNVFTREGNDLIATQKISLAEALAGCTVNLTTLDGRHLTVVINNVVHPEY 242
Query: 78 VRKFKGEGMPL-HTSTKKGDLYVTFEVLFPTTLSEEQKTKIKSIL 121
EGMPL TKKG+L + F + FPT L+ +QK +K +L
Sbjct: 243 EEVVPREGMPLPKDPTKKGNLRIKFNIKFPTRLTSDQKAGMKKVL 377
>TC77626 homologue to GP|5802244|gb|AAD51625.1| seed maturation protein PM37
{Glycine max}, partial (98%)
Length = 1675
Score = 69.7 bits (169), Expect = 3e-13
Identities = 40/124 (32%), Positives = 66/124 (52%), Gaps = 3/124 (2%)
Frame = +1
Query: 1 EVLFYEDGEPIIDGESGDLRFRIRTAPHELFKREGNDLHTTVTITLVQALVGFEKTIKHL 60
++ F + + D +GD+ F ++ H FKR+G DL T++L +AL GF+ + HL
Sbjct: 841 KITFPGEADEAPDTVTGDIVFVLQQKEHPKFKRKGEDLFVEHTLSLTEALCGFQFALTHL 1020
Query: 61 DEHLVDISSK--GITNPKQVRKFKGEGMPLHTST-KKGDLYVTFEVLFPTTLSEEQKTKI 117
D + I S + P + EGMP++ KG LY+ F V FP +L+ +Q +
Sbjct: 1021DSRQLLIKSNPGEVVKPDSYKAINDEGMPMYQRPFMKGKLYIHFTVEFPESLTLDQVKAL 1200
Query: 118 KSIL 121
++IL
Sbjct: 1201ETIL 1212
>TC86029 homologue to GP|5802244|gb|AAD51625.1| seed maturation protein PM37
{Glycine max}, complete
Length = 1572
Score = 68.6 bits (166), Expect = 6e-13
Identities = 39/124 (31%), Positives = 66/124 (52%), Gaps = 3/124 (2%)
Frame = +1
Query: 1 EVLFYEDGEPIIDGESGDLRFRIRTAPHELFKREGNDLHTTVTITLVQALVGFEKTIKHL 60
++ F + + D +GD+ F ++ H FKR+ DL T++L +AL GF+ + HL
Sbjct: 784 KITFPGEADEAPDTVTGDIVFVLQQKEHPKFKRKSEDLFVEHTLSLTEALCGFQFVLTHL 963
Query: 61 D--EHLVDISSKGITNPKQVRKFKGEGMPLHTST-KKGDLYVTFEVLFPTTLSEEQKTKI 117
D + L+ + + P + EGMP++ KG LY+ F V FP TLS +Q +
Sbjct: 964 DGRQLLIKSNPGEVVKPDSYKAINDEGMPMYQRPFMKGKLYIHFTVEFPDTLSLDQVKGL 1143
Query: 118 KSIL 121
+++L
Sbjct: 1144EAVL 1155
>TC85313 PIR|T09338|T09338 DnaJ-like protein MsJ1 - alfalfa, complete
Length = 1927
Score = 66.6 bits (161), Expect = 2e-12
Identities = 42/126 (33%), Positives = 66/126 (52%), Gaps = 5/126 (3%)
Frame = +1
Query: 1 EVLFYEDGEPIIDGESGDLRFRIRTAPHELFKREGNDLHTTVTITLVQALVGFEKTIKHL 60
+++F + D +GD+ F ++ H F+RE +DLH ++L +AL GF+ + HL
Sbjct: 988 KIVFEGQADEAPDTITGDIVFVLQVKGHPKFRRERDDLHIEHNLSLTEALCGFQFNVTHL 1167
Query: 61 D--EHLVDISSKGITNPKQVRKFKGEGMPLH-TSTKKGDLYVTFEVLFPTT--LSEEQKT 115
D + LV + + P Q + EGMP H KG LY+ F V FP + LS Q
Sbjct: 1168 DGRQLLVKSNPGEVIKPGQHKAINDEGMPQHGRPFMKGRLYIKFSVDFPDSGFLSPSQSL 1347
Query: 116 KIKSIL 121
+++ IL
Sbjct: 1348 ELEKIL 1365
>BG644408 homologue to PIR|T07371|T07 dnaJ protein homolog - potato, partial
(34%)
Length = 582
Score = 63.9 bits (154), Expect = 1e-11
Identities = 35/94 (37%), Positives = 54/94 (57%), Gaps = 3/94 (3%)
Frame = +1
Query: 31 FKREGNDLHTTVTITLVQALVGFEKTIKHLDEHLVDISSK--GITNPKQVRKFKGEGMPL 88
FKR+G+DL T++L +AL GF+ + HLD + I + + P Q + EGMP+
Sbjct: 13 FKRKGDDLFVEHTLSLTEALCGFQFILTHLDNRQLIIKPQPGEVVKPDQFKAINDEGMPM 192
Query: 89 HTST-KKGDLYVTFEVLFPTTLSEEQKTKIKSIL 121
+ +G LY+ F V FP TLS EQ ++++L
Sbjct: 193 YQRPFTRGKLYIHFTVEFPDTLSPEQCKNLEAVL 294
>TC87379 similar to PIR|T04618|T04618 heat shock protein homolog F20O9.160 -
Arabidopsis thaliana, partial (75%)
Length = 1431
Score = 50.1 bits (118), Expect = 2e-07
Identities = 33/124 (26%), Positives = 60/124 (47%), Gaps = 8/124 (6%)
Frame = +2
Query: 1 EVLFYEDGEPIIDGESGDLRFRIRTAPHELFKREGNDLHTTVTITLVQALVGFEKTIKHL 60
++ F E G + + D+ F I PH +F R+GNDL T I L + E +
Sbjct: 743 KITFPEKGNEQPNVIAADIVFVIDEKPHNVFTRQGNDLVMTQKILLAEG----EALSRSY 910
Query: 61 DEHLVDISSKGIT-------NPKQVRKFKGEGMPLHTS-TKKGDLYVTFEVLFPTTLSEE 112
L + +G+T +P GEGMP+ + +++G+L + F++ FP+ + E
Sbjct: 911 TFQLTTLDGRGLTIAIDNGIDPTYEEVIAGEGMPISKNPSQRGNLRIKFDITFPSMVDAE 1090
Query: 113 QKTK 116
+++
Sbjct: 1091TESQ 1102
>TC78760 similar to PIR|T48161|T48161 heat shock protein 40-like -
Arabidopsis thaliana, partial (56%)
Length = 762
Score = 46.2 bits (108), Expect = 3e-06
Identities = 25/51 (49%), Positives = 32/51 (62%), Gaps = 1/51 (1%)
Frame = +1
Query: 72 ITNPKQVRKFKGEGMPLHTS-TKKGDLYVTFEVLFPTTLSEEQKTKIKSIL 121
I +P KGEGMP+ +KKG+L V F V FP+ L+ EQKT IK +L
Sbjct: 562 IISPSYEEVIKGEGMPIPKEPSKKGNLRVKFNVKFPSRLTSEQKTGIKRLL 714
>TC81458 similar to GP|21553367|gb|AAM62460.1 DnaJ protein-like {Arabidopsis
thaliana}, partial (38%)
Length = 1178
Score = 43.9 bits (102), Expect = 1e-05
Identities = 30/115 (26%), Positives = 54/115 (46%), Gaps = 1/115 (0%)
Frame = +2
Query: 5 YEDGEPIIDGES-GDLRFRIRTAPHELFKREGNDLHTTVTITLVQALVGFEKTIKHLDEH 63
Y G +G+ GDL I+ +F+REG+D+H +++ QA++G + L
Sbjct: 368 YRSGGADPEGDRPGDLYVTIKVREDPVFRREGSDIHVDTVLSITQAILGGTIQVPTLTGD 547
Query: 64 LVDISSKGITNPKQVRKFKGEGMPLHTSTKKGDLYVTFEVLFPTTLSEEQKTKIK 118
+V G + ++V K +G+ G+ YV F P L++ Q+ I+
Sbjct: 548 VVLKVRPGTQHGQKV-VLKQKGIKTKKYNSLGNQYVHFIFFIPVNLTDRQRELIE 709
>TC77858 similar to GP|20197886|gb|AAD22362.2 putative DnaJ protein
{Arabidopsis thaliana}, partial (78%)
Length = 1869
Score = 41.6 bits (96), Expect = 7e-05
Identities = 26/108 (24%), Positives = 51/108 (47%), Gaps = 1/108 (0%)
Frame = +1
Query: 14 GESGDLRFRIRTAPHELFKREGNDLHTTVTITLVQALVGFEKTIKHLDEHLVDISSKGIT 73
G GDL + P + KRE ++ + ++ + A++G + +D +VD+ T
Sbjct: 1210 GSPGDLFVVLEVIPDPVLKREDTNILYSCKVSYIDAILGTTIKVPTVD-GMVDLKIPSGT 1386
Query: 74 NPKQVRKFKGEGMP-LHTSTKKGDLYVTFEVLFPTTLSEEQKTKIKSI 120
P +G+P L+ +GD +V +V P LS +++ I+ +
Sbjct: 1387 QPGSTLVMAKKGVPLLNKKNMRGDQFVRVQVEIPKKLSSDERKLIEEL 1530
>TC90708 weakly similar to PIR|S26703|S26703 dnaJ protein homolog YDJ1 - yeast
(Saccharomyces cerevisiae), partial (43%)
Length = 1098
Score = 26.9 bits (58), Expect = 1.9
Identities = 13/38 (34%), Positives = 21/38 (55%)
Frame = +2
Query: 1 EVLFYEDGEPIIDGESGDLRFRIRTAPHELFKREGNDL 38
+V F +G+ ++GD+ F I PH F R+ +DL
Sbjct: 968 KVEFRGEGDQAPGIQAGDVVFEIEQKPHTRFTRKDDDL 1081
>BQ150974
Length = 918
Score = 26.6 bits (57), Expect = 2.4
Identities = 14/33 (42%), Positives = 18/33 (54%)
Frame = +2
Query: 19 LRFRIRTAPHELFKREGNDLHTTVTITLVQALV 51
L FR TAPH +DL TVT++L +V
Sbjct: 335 LIFRSPTAPHRRHVHRASDLLMTVTMSLFSHIV 433
>BE321051 homologue to GP|15293149|gb| putative HSP protein {Arabidopsis
thaliana}, partial (2%)
Length = 177
Score = 26.2 bits (56), Expect = 3.2
Identities = 14/43 (32%), Positives = 22/43 (50%)
Frame = -2
Query: 63 HLVDISSKGITNPKQVRKFKGEGMPLHTSTKKGDLYVTFEVLF 105
HLV + +KG+T P V F S G +Y++F ++F
Sbjct: 143 HLVKLLNKGLTVPHSVETFSNSSRISIFSL--GGIYISF*IIF 21
>TC80077 similar to GP|16604607|gb|AAL24096.1 putative AtBgamma protein
{Arabidopsis thaliana}, partial (50%)
Length = 1338
Score = 25.8 bits (55), Expect = 4.2
Identities = 12/26 (46%), Positives = 16/26 (61%)
Frame = -2
Query: 44 ITLVQALVGFEKTIKHLDEHLVDISS 69
I L ++ +EK + HL EHL DI S
Sbjct: 860 ILLKVSMTRYEKLLIHLQEHLSDIMS 783
>BG454163
Length = 643
Score = 25.8 bits (55), Expect = 4.2
Identities = 12/40 (30%), Positives = 22/40 (55%)
Frame = -2
Query: 44 ITLVQALVGFEKTIKHLDEHLVDISSKGITNPKQVRKFKG 83
I +++ L + T+KHL+E + IS G + + F+G
Sbjct: 555 IQIIKRLKEKQSTLKHLNERSIKISIIGFSTEFEFYSFQG 436
>TC76825 homologue to SP|O81361|RS8_PRUAR 40S ribosomal protein S8.
[Apricot] {Prunus armeniaca}, partial (94%)
Length = 967
Score = 25.8 bits (55), Expect = 4.2
Identities = 14/45 (31%), Positives = 24/45 (53%)
Frame = -2
Query: 48 QALVGFEKTIKHLDEHLVDISSKGITNPKQVRKFKGEGMPLHTST 92
Q +VG + H+++ +S G T+P +V + + PLH ST
Sbjct: 345 QLIVG--SIVHHIEQ--TGFASDGFTSP*EVTGIQSQSSPLHIST 223
>TC78420 similar to GP|20453070|gb|AAM19780.1 AT5g66920/MUD21_18
{Arabidopsis thaliana}, partial (86%)
Length = 1855
Score = 25.4 bits (54), Expect = 5.5
Identities = 9/28 (32%), Positives = 17/28 (60%)
Frame = +3
Query: 38 LHTTVTITLVQALVGFEKTIKHLDEHLV 65
+HT + I+L ++G TI+H + H +
Sbjct: 552 IHTLMEISLCSLVIGTRPTIRH*ESHWI 635
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.317 0.138 0.386
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 3,085,624
Number of Sequences: 36976
Number of extensions: 34645
Number of successful extensions: 163
Number of sequences better than 10.0: 45
Number of HSP's better than 10.0 without gapping: 156
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 156
length of query: 121
length of database: 9,014,727
effective HSP length: 84
effective length of query: 37
effective length of database: 5,908,743
effective search space: 218623491
effective search space used: 218623491
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 52 (24.6 bits)
Medicago: description of AC145329.3