BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC145222.7 - phase: 0
(435 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BM814761 similar to GP|16604571|gb unknown protein {Arabidopsis ... 374 e-104
TC93207 similar to GP|16604571|gb|AAL24087.1 unknown protein {Ar... 268 3e-72
BE320540 similar to GP|16604571|gb unknown protein {Arabidopsis ... 234 6e-62
TC83565 similar to GP|16604571|gb|AAL24087.1 unknown protein {Ar... 155 2e-53
BF647285 117 7e-27
BF631984 similar to PIR|E86188|E861 hypothetical protein [import... 55 4e-08
TC78100 similar to SP|P32291|FD3E_PHAAU Omega-3 fatty acid desat... 30 1.4
BI268395 similar to PIR|F71440|F714 probable glucanase - Arabido... 30 2.4
BQ151380 homologue to GP|1504034|dbj| KIAA0227 {Homo sapiens}, p... 29 4.1
TC87567 similar to GP|17065112|gb|AAL32710.1 Unknown protein {Ar... 28 5.3
TC77574 similar to GP|14532862|gb|AAK64113.1 putative pyrophosph... 28 5.3
AW574038 weakly similar to GP|2584795|emb|C MtN27 {Medicago trun... 28 6.9
TC92005 weakly similar to GP|18033111|gb|AAL56987.1 functional c... 28 6.9
AL382096 weakly similar to GP|9624461|gb putative calcium transp... 28 6.9
TC77189 homologue to GP|13124865|gb|AAK11734.1 serine/threonine/... 28 9.0
TC80799 similar to GP|15810137|gb|AAL07212.1 unknown protein {Ar... 28 9.0
>BM814761 similar to GP|16604571|gb unknown protein {Arabidopsis thaliana},
partial (53%)
Length = 674
Score = 374 bits (960), Expect = e-104
Identities = 177/224 (79%), Positives = 199/224 (88%), Gaps = 4/224 (1%)
Frame = +1
Query: 153 YGTRVPLSSILPQVQMEAVLWGLGTAIGELPPYFISRAARLSGSRMDAMEELDSEDKGV- 211
+G+++PLS IL QVQ+EAVLWG+GTAIGELPPYFISRAARLSGS++DAMEELD+E++G+
Sbjct: 1 HGSQIPLSIILIQVQLEAVLWGMGTAIGELPPYFISRAARLSGSKLDAMEELDTENEGII 180
Query: 212 ---LNQIKCWFFSHTQHLNFFTILVLASVPNPLFDLAGIMCGQFGIPFWNFFLATLIGKA 268
LN+IK WF SH HLNF TIL LASVPNPLFDLAGIMCGQFG+PFW FFLAT IGKA
Sbjct: 181 TTYLNRIKRWFLSHCHHLNFLTILALASVPNPLFDLAGIMCGQFGVPFWKFFLATFIGKA 360
Query: 269 IIKTHIQTVFIISVCNNQLLDWIENEFIWVLSHIPGFASVLPKLTANLHAMKDKYLKAPH 328
+IKTHIQTVFIISVCNNQLL+WIENEFIWVL HIP FAS+LP + ANLHA+KDKYLKAPH
Sbjct: 361 LIKTHIQTVFIISVCNNQLLNWIENEFIWVLGHIPAFASILPSVIANLHAVKDKYLKAPH 540
Query: 329 PVSPNTKGKKWDFSFTSIWNTVVWLMLMNFFIKIVNSTAQTHLK 372
VSP KG +WDFS SIWNTVVWLMLMNFF+KIVNSTAQ +LK
Sbjct: 541 QVSPKIKGTRWDFSIASIWNTVVWLMLMNFFVKIVNSTAQRYLK 672
>TC93207 similar to GP|16604571|gb|AAL24087.1 unknown protein {Arabidopsis
thaliana}, partial (28%)
Length = 742
Score = 268 bits (685), Expect = 3e-72
Identities = 132/132 (100%), Positives = 132/132 (100%)
Frame = +3
Query: 1 MELENLTLTTQPFKTLKYSTLAVIQYIKKTMLYLLAKGGWLMLFSVAVGTLGVVLMTLGC 60
MELENLTLTTQPFKTLKYSTLAVIQYIKKTMLYLLAKGGWLMLFSVAVGTLGVVLMTLGC
Sbjct: 342 MELENLTLTTQPFKTLKYSTLAVIQYIKKTMLYLLAKGGWLMLFSVAVGTLGVVLMTLGC 521
Query: 61 LHEKHLEEFLEYFRFGLWWVALGVASSIGLGSGLHTFVLYLGPHIALFTIKAMQCGRVDL 120
LHEKHLEEFLEYFRFGLWWVALGVASSIGLGSGLHTFVLYLGPHIALFTIKAMQCGRVDL
Sbjct: 522 LHEKHLEEFLEYFRFGLWWVALGVASSIGLGSGLHTFVLYLGPHIALFTIKAMQCGRVDL 701
Query: 121 KSAPYDTIQLNR 132
KSAPYDTIQLNR
Sbjct: 702 KSAPYDTIQLNR 737
>BE320540 similar to GP|16604571|gb unknown protein {Arabidopsis thaliana},
partial (25%)
Length = 339
Score = 234 bits (596), Expect = 6e-62
Identities = 112/112 (100%), Positives = 112/112 (100%)
Frame = +3
Query: 182 LPPYFISRAARLSGSRMDAMEELDSEDKGVLNQIKCWFFSHTQHLNFFTILVLASVPNPL 241
LPPYFISRAARLSGSRMDAMEELDSEDKGVLNQIKCWFFSHTQHLNFFTILVLASVPNPL
Sbjct: 3 LPPYFISRAARLSGSRMDAMEELDSEDKGVLNQIKCWFFSHTQHLNFFTILVLASVPNPL 182
Query: 242 FDLAGIMCGQFGIPFWNFFLATLIGKAIIKTHIQTVFIISVCNNQLLDWIEN 293
FDLAGIMCGQFGIPFWNFFLATLIGKAIIKTHIQTVFIISVCNNQLLDWIEN
Sbjct: 183 FDLAGIMCGQFGIPFWNFFLATLIGKAIIKTHIQTVFIISVCNNQLLDWIEN 338
>TC83565 similar to GP|16604571|gb|AAL24087.1 unknown protein {Arabidopsis
thaliana}, partial (27%)
Length = 598
Score = 155 bits (391), Expect(3) = 2e-53
Identities = 72/94 (76%), Positives = 81/94 (85%)
Frame = +2
Query: 23 VIQYIKKTMLYLLAKGGWLMLFSVAVGTLGVVLMTLGCLHEKHLEEFLEYFRFGLWWVAL 82
+IQY+KKT YLLAKGG LML SV VG LG++L+T+ HEKH EE L+YFRFGLWW+ L
Sbjct: 233 IIQYLKKTATYLLAKGGLLMLLSVVVGALGIMLITVEGPHEKHFEELLKYFRFGLWWITL 412
Query: 83 GVASSIGLGSGLHTFVLYLGPHIALFTIKAMQCG 116
G+ASSIGLGSGLHTFVLYLGPHIA FTIKAMQCG
Sbjct: 413 GIASSIGLGSGLHTFVLYLGPHIAFFTIKAMQCG 514
Score = 52.8 bits (125), Expect(3) = 2e-53
Identities = 23/33 (69%), Positives = 26/33 (78%)
Frame = +3
Query: 111 KAMQCGRVDLKSAPYDTIQLNRGPSWLDKNCSQ 143
K RVDL SAPYDTIQL+RGP+WLD +CSQ
Sbjct: 498 KPCSVARVDLXSAPYDTIQLDRGPTWLDMDCSQ 596
Score = 40.8 bits (94), Expect(3) = 2e-53
Identities = 18/29 (62%), Positives = 24/29 (82%)
Frame = +1
Query: 1 MELENLTLTTQPFKTLKYSTLAVIQYIKK 29
+EL+NLTLTTQPFKTLK+ TLA +++
Sbjct: 166 LELDNLTLTTQPFKTLKFFTLAYYSILEE 252
>BF647285
Length = 338
Score = 117 bits (294), Expect = 7e-27
Identities = 63/67 (94%), Positives = 63/67 (94%)
Frame = -3
Query: 369 THLKKQQESEVAALTKKSDSDTQ*NALIIIFAVHPARSKLKFKFQLFLKPPMEVTP*NLL 428
THLKKQQESEVAALTKKSDSDTQ*NALIIIFAVHPAR KLKFKFQLFLKPPMEVTP*N
Sbjct: 336 THLKKQQESEVAALTKKSDSDTQ*NALIIIFAVHPARXKLKFKFQLFLKPPMEVTP*NFF 157
Query: 429 ***IHRV 435
***I RV
Sbjct: 156 ***IXRV 136
>BF631984 similar to PIR|E86188|E861 hypothetical protein [imported] -
Arabidopsis thaliana, partial (6%)
Length = 220
Score = 55.5 bits (132), Expect = 4e-08
Identities = 22/29 (75%), Positives = 26/29 (88%)
Frame = +2
Query: 64 KHLEEFLEYFRFGLWWVALGVASSIGLGS 92
+H EE L+YFRFGLWW+ LG+ASSIGLGS
Sbjct: 134 QHFEELLKYFRFGLWWITLGIASSIGLGS 220
>TC78100 similar to SP|P32291|FD3E_PHAAU Omega-3 fatty acid desaturase
endoplasmic reticulum (EC 1.14.99.-), partial (96%)
Length = 1343
Score = 30.4 bits (67), Expect = 1.4
Identities = 20/81 (24%), Positives = 35/81 (42%), Gaps = 2/81 (2%)
Frame = +2
Query: 183 PPYFISRAARLSGSRMDAMEEL--DSEDKGVLNQIKCWFFSHTQHLNFFTILVLASVPNP 240
P Y +R+ GS + +L SE K V+ CW + F + L+ + P
Sbjct: 638 PFYLWNRSPGKEGSHFNPYSKLFTPSERKDVITSTVCW------SIMFCLLTYLSLIKGP 799
Query: 241 LFDLAGIMCGQFGIPFWNFFL 261
+ +M +G+P+W F +
Sbjct: 800 I-----VMLKLYGVPYWIFIM 847
>BI268395 similar to PIR|F71440|F714 probable glucanase - Arabidopsis
thaliana, partial (11%)
Length = 377
Score = 29.6 bits (65), Expect = 2.4
Identities = 22/78 (28%), Positives = 35/78 (44%)
Frame = +2
Query: 56 MTLGCLHEKHLEEFLEYFRFGLWWVALGVASSIGLGSGLHTFVLYLGPHIALFTIKAMQC 115
MT C + + ++ FGL WV L S+IG+ G +F L P + +K +
Sbjct: 122 MTEPCCNRFGVGNLVKIMMFGLCWVPLLTESAIGVNWGTVSF-RKLNPSTVVDLLKDNKI 298
Query: 116 GRVDLKSAPYDTIQLNRG 133
+V L A D ++ G
Sbjct: 299 TKVKLFEAEADVLKALMG 352
>BQ151380 homologue to GP|1504034|dbj| KIAA0227 {Homo sapiens}, partial (4%)
Length = 1185
Score = 28.9 bits (63), Expect = 4.1
Identities = 22/61 (36%), Positives = 31/61 (50%), Gaps = 1/61 (1%)
Frame = -3
Query: 34 LLAKGGWLMLF-SVAVGTLGVVLMTLGCLHEKHLEEFLEYFRFGLWWVALGVASSIGLGS 92
L+ +G M F SV V G V +TL L + +F GL WV++GVA + +G
Sbjct: 1060 LVIRGSRSMPFLSVPVSLCGRVRVTLASLSISSYVCAVVFFFAGLIWVSMGVALAGAVGW 881
Query: 93 G 93
G
Sbjct: 880 G 878
>TC87567 similar to GP|17065112|gb|AAL32710.1 Unknown protein {Arabidopsis
thaliana}, partial (82%)
Length = 2117
Score = 28.5 bits (62), Expect = 5.3
Identities = 18/61 (29%), Positives = 28/61 (45%), Gaps = 2/61 (3%)
Frame = +3
Query: 80 VALGVASSIGLGSGLHT--FVLYLGPHIALFTIKAMQCGRVDLKSAPYDTIQLNRGPSWL 137
V L S+ + SGL + F++YL H + TI + CG+ + LN W+
Sbjct: 771 VPLPAQLSLVISSGLISKEFIIYL-KHFRITTINWLSCGQGKAAKVSLQLLNLNLNLHWM 947
Query: 138 D 138
D
Sbjct: 948 D 950
>TC77574 similar to GP|14532862|gb|AAK64113.1 putative pyrophosphate-dependent
phosphofructo-1-kinase {Arabidopsis thaliana}, partial
(87%)
Length = 1926
Score = 28.5 bits (62), Expect = 5.3
Identities = 15/47 (31%), Positives = 26/47 (54%), Gaps = 7/47 (14%)
Frame = +3
Query: 127 TIQLNRGPSWLD-------KNCSQFGPPLFQSEYGTRVPLSSILPQV 166
T++L + P+ D ++C + G P+F+ YGT ++I PQV
Sbjct: 1581 TLRLMKSPTIWDA*LRLRYQSCVREGYPVFKKNYGTGTSTAAISPQV 1721
>AW574038 weakly similar to GP|2584795|emb|C MtN27 {Medicago truncatula},
partial (45%)
Length = 707
Score = 28.1 bits (61), Expect = 6.9
Identities = 13/30 (43%), Positives = 19/30 (63%), Gaps = 2/30 (6%)
Frame = -1
Query: 253 GIPFWNFFLATLIGKAIIKT--HIQTVFII 280
G+ W FF +TL+GKA T +Q +FI+
Sbjct: 251 GLAKWKFFFSTLVGKAQTMTANALQNIFIM 162
>TC92005 weakly similar to GP|18033111|gb|AAL56987.1 functional candidate
resistance protein KR1 {Glycine max}, partial (7%)
Length = 858
Score = 28.1 bits (61), Expect = 6.9
Identities = 21/80 (26%), Positives = 34/80 (42%)
Frame = +1
Query: 241 LFDLAGIMCGQFGIPFWNFFLATLIGKAIIKTHIQTVFIISVCNNQLLDWIENEFIWVLS 300
L D++ CG P N + +++ + + +Q + C LL W N LS
Sbjct: 346 LNDISIERCGMLRFPKHNDKINSIVFSNVTQLSLQNCNLSDECLPILLKWFVNVKRLDLS 525
Query: 301 HIPGFASVLPKLTANLHAMK 320
H F ++LP+ H MK
Sbjct: 526 HNFNF-NILPECLNECHLMK 582
>AL382096 weakly similar to GP|9624461|gb putative calcium transporting
ATPase {Ajellomyces capsulatus}, partial (9%)
Length = 456
Score = 28.1 bits (61), Expect = 6.9
Identities = 26/92 (28%), Positives = 42/92 (45%), Gaps = 7/92 (7%)
Frame = +3
Query: 228 FFTILVLASVPNPLFDL-----AGIMCGQF--GIPFWNFFLATLIGKAIIKTHIQTVFII 280
F+ L+L VP +F+ +G + G + G + LAT++GKA + T + T +
Sbjct: 129 FYHSLILYFVPVFIFNQDLVLSSGQLAGHWVWGTTIYTAVLATVLGKAALITDLWTKYTY 308
Query: 281 SVCNNQLLDWIENEFIWVLSHIPGFASVLPKL 312
I FI+ L I + S+ PKL
Sbjct: 309 IA--------IPGSFIFWLVFIVLYGSIAPKL 380
>TC77189 homologue to GP|13124865|gb|AAK11734.1 serine/threonine/tyrosine
kinase {Arachis hypogaea}, partial (95%)
Length = 1788
Score = 27.7 bits (60), Expect = 9.0
Identities = 12/38 (31%), Positives = 18/38 (46%)
Frame = -2
Query: 60 CLHEKHLEEFLEYFRFGLWWVALGVASSIGLGSGLHTF 97
C H+ +L F + F WW +GV G+ + H F
Sbjct: 1454 CCHDLNLSTFKHCYNFSKWWPNIGV----GIPAPSHDF 1353
>TC80799 similar to GP|15810137|gb|AAL07212.1 unknown protein {Arabidopsis
thaliana}, partial (41%)
Length = 1723
Score = 27.7 bits (60), Expect = 9.0
Identities = 13/34 (38%), Positives = 21/34 (61%), Gaps = 3/34 (8%)
Frame = +3
Query: 198 MDAMEELDSE---DKGVLNQIKCWFFSHTQHLNF 228
MD + E++S D+G LN+I W+ +H+NF
Sbjct: 1533 MDHINEIESYNGGDQGYLNEIFTWWHRIPRHMNF 1634
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.331 0.143 0.461
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 15,091,674
Number of Sequences: 36976
Number of extensions: 229255
Number of successful extensions: 1716
Number of sequences better than 10.0: 32
Number of HSP's better than 10.0 without gapping: 1705
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1714
length of query: 435
length of database: 9,014,727
effective HSP length: 99
effective length of query: 336
effective length of database: 5,354,103
effective search space: 1798978608
effective search space used: 1798978608
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.9 bits)
S2: 60 (27.7 bits)
Medicago: description of AC145222.7