BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC145222.13 + phase: 0
(411 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
CA918271 similar to PIR|T51513|T51 hypothetical protein F5E19_15... 470 e-133
BE942940 weakly similar to GP|13506749|gb receptor-like protein ... 36 0.024
TC78173 SP|P24923|CC21_MEDSA Cell division control protein 2 hom... 33 0.26
TC87516 PIR|T09591|T09591 probable cdc2-like protein kinase cdc2... 32 0.45
AL369682 similar to GP|17065208|gb Unknown protein {Arabidopsis ... 32 0.59
TC83306 similar to PIR|T48213|T48213 hypothetical protein T20L15... 31 0.77
TC92178 similar to GP|18086496|gb|AAL57701.1 AT4g37250/C7A10_110... 31 1.0
BE320157 weakly similar to PIR|T04835|T04 probable serine/threon... 30 1.7
TC87305 homologue to SP|Q05006|CC22_MEDSA Cell division control ... 30 1.7
TC82674 weakly similar to GP|19698923|gb|AAL91197.1 unknown prot... 29 3.8
TC92314 similar to GP|16904220|gb|AAL30817.1 calcium/calmodulin-... 29 3.8
TC87063 weakly similar to GP|4127461|emb|CAA09731.1 receptor-lik... 28 5.0
TC80630 similar to GP|22655010|gb|AAM98096.1 AT3g13690/MMM17_12 ... 28 5.0
TC88740 similar to PIR|S29851|S29851 protein kinase 6 (EC 2.7.1.... 28 6.5
TC86213 homologue to PIR|S60121|S60121 mitogen-activated protein... 28 6.5
TC86932 similar to GP|20466530|gb|AAM20582.1 protein kinase-like... 28 6.5
TC83734 homologue to PIR|S60121|S60121 mitogen-activated protein... 28 6.5
AW587133 similar to PIR|T02105|T02 calcium-dependent protein kin... 28 8.5
TC80078 similar to PIR|T02105|T02105 calcium-dependent protein k... 28 8.5
TC78103 SP|Q07176|MMK1_MEDSA Mitogen-activated protein kinase ho... 28 8.5
>CA918271 similar to PIR|T51513|T51 hypothetical protein F5E19_150 -
Arabidopsis thaliana, partial (41%)
Length = 722
Score = 470 bits (1210), Expect = e-133
Identities = 237/239 (99%), Positives = 237/239 (99%)
Frame = +1
Query: 28 VAAVPLLPSSIFFTSNKSKLRIKANYGIKFITNSDWFQVGRPIGNYGFMNVTTSSTDQYS 87
VAAVPLLPSSIFFTSNKSKLRIKANYGIKFITNSDWFQVGRPIGNYGFMNVTTSSTDQYS
Sbjct: 1 VAAVPLLPSSIFFTSNKSKLRIKANYGIKFITNSDWFQVGRPIGNYGFMNVTTSSTDQYS 180
Query: 88 LGGGLKTQDVQEGSVKIRLYEGRVSQGPLKQTPVLFKVYPGTRAGGVVADMMAANELNSH 147
LGGGLKTQDVQEGSVKIRLYEGRVSQGPLKQTPVLFKVYPGTRAGGVVADMMAANELNSH
Sbjct: 181 LGGGLKTQDVQEGSVKIRLYEGRVSQGPLKQTPVLFKVYPGTRAGGVVADMMAANELNSH 360
Query: 148 MFLQSSSKGISQHLMLLLGGFETTTGEQWLAFRDYGKSTAADYAKVASEKVSKLSSWNSF 207
MFLQSSSKGISQHLMLLLGGFETTTGEQWLAFRDYGKSTAADYAKVASEKVSKLSSWNSF
Sbjct: 361 MFLQSSSKGISQHLMLLLGGFETTTGEQWLAFRDYGKSTAADYAKVASEKVSKLSSWNSF 540
Query: 208 ERGQSMKRRRRFIIKLLQGALRGLAYMHDHDRLHQSLGPFSVSLNTISESEAPYLIPRL 266
ER QSMKRRRRFIIKLLQGALRGLAYMHDHDRLHQSLGPFSVSLNTISESEAPYLIP L
Sbjct: 541 ERSQSMKRRRRFIIKLLQGALRGLAYMHDHDRLHQSLGPFSVSLNTISESEAPYLIPDL 717
>BE942940 weakly similar to GP|13506749|gb receptor-like protein kinase 6
{Arabidopsis thaliana}, partial (23%)
Length = 466
Score = 36.2 bits (82), Expect = 0.024
Identities = 36/151 (23%), Positives = 61/151 (39%), Gaps = 3/151 (1%)
Frame = +1
Query: 173 GEQWLAFRDYGKSTAADYAKVASEKVSKLSSWNSFERGQSMKRRRRFIIKLLQGALRGLA 232
G + + +Y + + DY S + KL +W ER ++ G RG+
Sbjct: 46 GHEKILIYEYVSNKSLDYFLFDSHQ-QKLLTW--VER-----------FNIIGGIARGIL 183
Query: 233 YMHDHDRL---HQSLGPFSVSLNTISESEAPYLIPRLRDLAFSVSVRYSELEDSGPLTEG 289
Y+H+H RL H+ L P ++ L+ +IP++ D + V S+ E S G
Sbjct: 184 YLHEHSRLKVIHRDLKPSNILLD-------ENMIPKISDFGLARMVEISQEEGSTNRIVG 342
Query: 290 LWARASAASAFTYLEKRAFGIADDIYEAGLL 320
+ S Y F D+Y G++
Sbjct: 343 TYGYMSP----EYAMFGQFSEKSDVYSFGVM 423
>TC78173 SP|P24923|CC21_MEDSA Cell division control protein 2 homolog 1 (EC
2.7.1.-) (Fragment). [Alfalfa] {Medicago sativa},
complete
Length = 1268
Score = 32.7 bits (73), Expect = 0.26
Identities = 53/203 (26%), Positives = 84/203 (41%), Gaps = 15/203 (7%)
Frame = +2
Query: 218 RFIIKLLQGALRGLAYMHDHDRLHQSLGPFSVSLN--TISESEAPYLIPRLRDLAFSVSV 275
R + L L G+AY H H LH+ L P ++ ++ T S A + + R AF + V
Sbjct: 431 RQVKMFLYQMLCGIAYCHSHRVLHRDLKPQNLLIDRRTNSLKLADFGLAR----AFGIPV 598
Query: 276 RYSELEDSGPLTEGLWARASAASAFTYLEKRAFGIADDIYEAGLLFAYLA----FVPFCE 331
R E LW RA L R + D++ G +FA +A P +
Sbjct: 599 RTFTHE-----VVTLWYRAPE----ILLGSRHYSTPVDVWSVGCIFAEMANRRPLFP-GD 748
Query: 332 AGVMDGLSLQRLLEN---------TFRLDLEATREYCIADDKLVNAIEFLDLGDGAGWEL 382
+ + + + R+L T D ++T + D L + L + AG +L
Sbjct: 749 SEIDELFKIFRILGTPNEDTWPGVTSLPDFKSTFPRWPSKD-LATVVPNL---EPAGLDL 916
Query: 383 LQAMLNADFRKRPTAEAVLSHRF 405
L +ML D KR TA + + H +
Sbjct: 917 LNSMLCLDPTKRITARSAVEHEY 985
>TC87516 PIR|T09591|T09591 probable cdc2-like protein kinase cdc2MsF -
alfalfa, complete
Length = 1251
Score = 32.0 bits (71), Expect = 0.45
Identities = 47/198 (23%), Positives = 82/198 (40%), Gaps = 15/198 (7%)
Frame = +2
Query: 223 LLQGALRGLAYMHDHDRLHQSLGPFSVSLNTISESEAPYLIPRLRDL----AFSVSVRYS 278
L+ +G+A+ H H LH+ L P ++ + + ++ ++ DL AF+V ++
Sbjct: 443 LMYQLCKGVAFCHGHGILHRDLKPHNLLM------DRKTMMLKIADLGLARAFTVPLKKY 604
Query: 279 ELEDSGPLTEGLWARASAASAFTYLEKRAFGIADDIYEAGLLFAYLAFVPFCEAGVMDGL 338
E LT LW RA L + +A D++ +FA L + + G
Sbjct: 605 THE---ILT--LWYRAPE----VLLGATHYSMAVDMWSVACIFAELV----TKTALFPGD 745
Query: 339 SLQRLLENTFRLDLEATREYCIADDKLVNAIEFLDLG-----------DGAGWELLQAML 387
S + L + FRL + KL+N E+ G + G +LL ML
Sbjct: 746 SELQQLLHIFRLLGTPNEDVWPGVSKLMNWHEYPQWGPQSLSKAVPGLEETGVDLLSQML 925
Query: 388 NADFRKRPTAEAVLSHRF 405
+ KR +A+ + H +
Sbjct: 926 QYEPSKRLSAKKAMEHPY 979
>AL369682 similar to GP|17065208|gb Unknown protein {Arabidopsis thaliana},
partial (15%)
Length = 536
Score = 31.6 bits (70), Expect = 0.59
Identities = 29/146 (19%), Positives = 57/146 (38%), Gaps = 3/146 (2%)
Frame = -1
Query: 182 YGKSTAADYAKVASEKVSKLSSWNSFERGQSMKRRRRFIIKLLQGALRGLAYMH---DHD 238
Y T+ D + + E + S W+ M+ ++ GA +GL Y+H +
Sbjct: 410 YCSITSEDSSLLVYEYLPNGSLWDRLHSSGKMELDWETRYEIAVGAAKGLEYLHHGCERP 231
Query: 239 RLHQSLGPFSVSLNTISESEAPYLIPRLRDLAFSVSVRYSELEDSGPLTEGLWARASAAS 298
+H+ + ++ L+ +L PR+ D + V ++DS + G +
Sbjct: 230 VIHRDVKSSNILLD-------EFLKPRIADFGLAKIVHADVVKDSTHIIAGTHGYIAPEY 72
Query: 299 AFTYLEKRAFGIADDIYEAGLLFAYL 324
+TY D+Y G++ L
Sbjct: 71 GYTYRVNE----KSDVYSFGVVLMEL 6
>TC83306 similar to PIR|T48213|T48213 hypothetical protein T20L15.190 -
Arabidopsis thaliana, partial (39%)
Length = 978
Score = 31.2 bits (69), Expect = 0.77
Identities = 24/79 (30%), Positives = 37/79 (46%), Gaps = 3/79 (3%)
Frame = +2
Query: 171 TTGEQWLAFRDYGKSTAADYAKVASEKVSKLSS--WNSFERGQSMKRRRRFIIK-LLQGA 227
T G +WL ++ G T ADY K + S L S + +G RR IIK +++
Sbjct: 734 TKGGKWLVWKFEGDRTLADYMKERNFP-SNLESVMFGRVLQGVDSSRRNALIIKQIMRQI 910
Query: 228 LRGLAYMHDHDRLHQSLGP 246
+ L +HD +H+ P
Sbjct: 911 ITSLKKIHDTGIVHREYKP 967
>TC92178 similar to GP|18086496|gb|AAL57701.1 AT4g37250/C7A10_110
{Arabidopsis thaliana}, partial (33%)
Length = 1004
Score = 30.8 bits (68), Expect = 1.0
Identities = 12/36 (33%), Positives = 21/36 (58%)
Frame = +2
Query: 221 IKLLQGALRGLAYMHDHDRLHQSLGPFSVSLNTISE 256
+K+ +G RGL Y+H+ +H +L P ++ L E
Sbjct: 599 LKIAKGVARGLTYLHEKKHVHGNLKPTNILLGNDME 706
>BE320157 weakly similar to PIR|T04835|T04 probable serine/threonine-specific
protein kinase (EC 2.7.1.-) F21P8.70 - Arabidopsis
thaliana, partial (27%)
Length = 529
Score = 30.0 bits (66), Expect = 1.7
Identities = 27/94 (28%), Positives = 46/94 (48%), Gaps = 3/94 (3%)
Frame = +1
Query: 162 MLLLGGFETTTGEQWLAFRDYGKSTAADYAKVASEKVSKLSSWNSFERGQSMKRRRRFII 221
++ L GF E+ L + +Y + + DY S+K ++ W FER
Sbjct: 226 LVTLLGFCLEEREKMLIY-EYVPNKSLDYFLFDSKK-HRVLHW--FER-----------Y 360
Query: 222 KLLQGALRGLAYMHDHDRL---HQSLGPFSVSLN 252
K++ G RG+ Y+H++ RL H+ L P +V L+
Sbjct: 361 KIIGGIARGILYLHEYSRLKVIHRDLKPSNVLLD 462
>TC87305 homologue to SP|Q05006|CC22_MEDSA Cell division control protein 2
homolog 2 (EC 2.7.1.-). [Alfalfa] {Medicago sativa},
complete
Length = 1378
Score = 30.0 bits (66), Expect = 1.7
Identities = 31/108 (28%), Positives = 46/108 (41%), Gaps = 2/108 (1%)
Frame = +2
Query: 217 RRFIIKLLQGALRGLAYMHDHDRLHQSLGPFSVSLNTISES--EAPYLIPRLRDLAFSVS 274
+R I L L G+AY H H LH+ L P ++ ++ S + A + + R AF +
Sbjct: 401 QRQIKMFLYQILCGIAYCHSHRVLHRDLKPQNLLIDRSSNALKLADFGLAR----AFGIP 568
Query: 275 VRYSELEDSGPLTEGLWARASAASAFTYLEKRAFGIADDIYEAGLLFA 322
VR E LW RA L R + D++ G +FA
Sbjct: 569 VRTFTHE-----VVTLWYRAPE----ILLGSRHYSTPVDVWSVGCIFA 685
>TC82674 weakly similar to GP|19698923|gb|AAL91197.1 unknown protein
{Arabidopsis thaliana}, partial (13%)
Length = 670
Score = 28.9 bits (63), Expect = 3.8
Identities = 17/40 (42%), Positives = 21/40 (52%)
Frame = +1
Query: 239 RLHQSLGPFSVSLNTISESEAPYLIPRLRDLAFSVSVRYS 278
R HQ+L PFS +L + S L RL S+S RYS
Sbjct: 460 RTHQNLSPFSSTLFSPFVSFGYVLATRLAKQVHSISTRYS 579
>TC92314 similar to GP|16904220|gb|AAL30817.1 calcium/calmodulin-dependent
protein kinase CaMK4 {Arabidopsis thaliana}, partial
(54%)
Length = 1018
Score = 28.9 bits (63), Expect = 3.8
Identities = 12/31 (38%), Positives = 20/31 (63%)
Frame = +1
Query: 379 GWELLQAMLNADFRKRPTAEAVLSHRFMTGE 409
G + ++ +LN D+RKR TA L+H ++ E
Sbjct: 517 GKDFVKRLLNKDYRKRMTAAQALTHPWLRDE 609
>TC87063 weakly similar to GP|4127461|emb|CAA09731.1 receptor-like protein
kinase RLK3 {Arabidopsis thaliana}, partial (25%)
Length = 2063
Score = 28.5 bits (62), Expect = 5.0
Identities = 27/116 (23%), Positives = 50/116 (42%), Gaps = 3/116 (2%)
Frame = +3
Query: 208 ERGQSMKRRRRFIIKLLQGALRGLAYMHDHDRL---HQSLGPFSVSLNTISESEAPYLIP 264
E+ + RR+ K+++G RG+ Y+H+ RL H+ L ++ L+ L P
Sbjct: 804 EKAHQINWPRRY--KIIEGIARGMLYLHEDSRLRIIHRDLKASNILLD-------ENLNP 956
Query: 265 RLRDLAFSVSVRYSELEDSGPLTEGLWARASAASAFTYLEKRAFGIADDIYEAGLL 320
++ D + R ++ + +T + S Y F I D+Y G+L
Sbjct: 957 KISDFGMA---RIFGVDQTRGITNRVVGTLGYMSP-EYAMHGEFSIKTDVYSFGVL 1112
>TC80630 similar to GP|22655010|gb|AAM98096.1 AT3g13690/MMM17_12
{Arabidopsis thaliana}, partial (18%)
Length = 1044
Score = 28.5 bits (62), Expect = 5.0
Identities = 11/29 (37%), Positives = 20/29 (68%)
Frame = +2
Query: 221 IKLLQGALRGLAYMHDHDRLHQSLGPFSV 249
IK+ GA RGL Y+H ++ +H+ + P ++
Sbjct: 635 IKVAIGAARGLLYLHKNNIIHRDVRPNNI 721
>TC88740 similar to PIR|S29851|S29851 protein kinase 6 (EC 2.7.1.-) -
soybean, partial (75%)
Length = 1364
Score = 28.1 bits (61), Expect = 6.5
Identities = 22/92 (23%), Positives = 39/92 (41%)
Frame = +2
Query: 229 RGLAYMHDHDRLHQSLGPFSVSLNTISESEAPYLIPRLRDLAFSVSVRYSELEDSGPLTE 288
RG+ Y+H +H+ L P +V +N S ++ D + Y +L P T
Sbjct: 545 RGMEYIHSQGVIHRDLKPENVLINGDSHL-------KIADFGIACEEAYCDLLADDPGTY 703
Query: 289 GLWARASAASAFTYLEKRAFGIADDIYEAGLL 320
W A + ++++G D+Y GL+
Sbjct: 704 -RW------MAPEMIRRKSYGRKVDVYSFGLI 778
>TC86213 homologue to PIR|S60121|S60121 mitogen-activated protein kinase
MMK2 (EC 2.7.1.-) - alfalfa, complete
Length = 1596
Score = 28.1 bits (61), Expect = 6.5
Identities = 15/36 (41%), Positives = 22/36 (60%)
Frame = +2
Query: 217 RRFIIKLLQGALRGLAYMHDHDRLHQSLGPFSVSLN 252
R F+ +LL RGL Y+H + LH+ L P ++ LN
Sbjct: 662 RYFVYQLL----RGLKYVHSANVLHRDLKPSNLLLN 757
>TC86932 similar to GP|20466530|gb|AAM20582.1 protein kinase-like protein
{Arabidopsis thaliana}, partial (73%)
Length = 1464
Score = 28.1 bits (61), Expect = 6.5
Identities = 28/109 (25%), Positives = 42/109 (37%), Gaps = 5/109 (4%)
Frame = +3
Query: 220 IIKLLQGALRGLAYMHDHDRLHQSLGPFSVSLNTISESEAPYLI-----PRLRDLAFSVS 274
++ L L + YMH LH+ + P + + +S Y+I R RD S
Sbjct: 375 VLMLADQMLSRIEYMHSKGFLHRDIKPDNFLMGIRRKSSQVYIIDFGLGKRYRDPKTSKH 554
Query: 275 VRYSELEDSGPLTEGLWARASAASAFTYLEKRAFGIADDIYEAGLLFAY 323
+ Y E + L A AS T+L G DD+ G + Y
Sbjct: 555 IPYRE-------NKSLTGTARYASCNTHLGIEQ-GRRDDLESIGYVLMY 677
>TC83734 homologue to PIR|S60121|S60121 mitogen-activated protein kinase
MMK2 (EC 2.7.1.-) - alfalfa, partial (76%)
Length = 925
Score = 28.1 bits (61), Expect = 6.5
Identities = 12/26 (46%), Positives = 18/26 (69%)
Frame = +2
Query: 228 LRGLAYMHDHDRLHQSLGPFSVSLNT 253
LRGL Y+H + LH+ L P ++ LN+
Sbjct: 488 LRGLKYVHSANVLHRDLKPSNLLLNS 565
>AW587133 similar to PIR|T02105|T02 calcium-dependent protein kinase (EC
2.7.1.-) T3K9.9 - Arabidopsis thaliana, partial (33%)
Length = 661
Score = 27.7 bits (60), Expect = 8.5
Identities = 11/30 (36%), Positives = 19/30 (62%)
Frame = +3
Query: 381 ELLQAMLNADFRKRPTAEAVLSHRFMTGEV 410
+ ++ +LN D+RKR TA LSH ++ +
Sbjct: 204 DFVKRLLNKDYRKRLTAAQALSHPWLVNHL 293
>TC80078 similar to PIR|T02105|T02105 calcium-dependent protein kinase (EC
2.7.1.-) T3K9.9 - Arabidopsis thaliana, partial (47%)
Length = 1326
Score = 27.7 bits (60), Expect = 8.5
Identities = 11/30 (36%), Positives = 19/30 (62%)
Frame = +3
Query: 381 ELLQAMLNADFRKRPTAEAVLSHRFMTGEV 410
+ ++ +LN D+RKR TA LSH ++ +
Sbjct: 189 DFVKRLLNKDYRKRLTAAQALSHPWLVNHL 278
>TC78103 SP|Q07176|MMK1_MEDSA Mitogen-activated protein kinase homolog MMK1
(EC 2.7.1.-) (MAP kinase MSK7) (MAP kinase ERK1).,
partial (65%)
Length = 923
Score = 27.7 bits (60), Expect = 8.5
Identities = 12/25 (48%), Positives = 17/25 (68%)
Frame = +1
Query: 228 LRGLAYMHDHDRLHQSLGPFSVSLN 252
LRGL Y+H + LH+ L P ++ LN
Sbjct: 631 LRGLKYIHSANVLHRDLKPSNLLLN 705
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.319 0.135 0.391
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 10,891,687
Number of Sequences: 36976
Number of extensions: 132701
Number of successful extensions: 703
Number of sequences better than 10.0: 41
Number of HSP's better than 10.0 without gapping: 698
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 703
length of query: 411
length of database: 9,014,727
effective HSP length: 98
effective length of query: 313
effective length of database: 5,391,079
effective search space: 1687407727
effective search space used: 1687407727
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 59 (27.3 bits)
Medicago: description of AC145222.13