BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC145219.5 + phase: 0
(130 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC80100 similar to GP|21618014|gb|AAM67064.1 unknown {Arabidopsi... 264 6e-72
AJ501135 similar to GP|4680489|gb|A hypothetical protein {Oryza ... 85 7e-18
AL388398 similar to GP|8778455|gb|A F1L3.15 {Arabidopsis thalian... 83 3e-17
TC90077 similar to GP|8778455|gb|AAF79463.1| F1L3.15 {Arabidopsi... 79 5e-16
BF519497 similar to GP|20197919|gb| Expressed protein {Arabidops... 70 3e-13
AW773655 similar to SP|Q93WT0|WR31_ Probable WRKY transcription ... 33 0.032
TC88569 homologue to SP|Q9C519|WRK6_ARATH WRKY transcription fac... 33 0.032
TC87120 similar to SP|P48724|IF5_PHAVU Eukaryotic translation in... 30 0.35
TC81168 similar to GP|17979303|gb|AAL49877.1 unknown protein {Ar... 30 0.35
TC91110 similar to GP|7576941|gb|AAF64065.1| putative diacylglyc... 29 0.46
BQ151193 similar to GP|13359187|dbj KIAA1657 protein {Homo sapie... 29 0.46
TC78741 weakly similar to GP|21593418|gb|AAM65385.1 unknown {Ara... 29 0.60
TC76402 weakly similar to GP|18086561|gb|AAL57705.1 At2g01100/F2... 28 0.78
BQ141748 homologue to SP|P21997|SSGP_ Sulfated surface glycoprot... 27 1.7
TC77368 similar to GP|14596081|gb|AAK68768.1 putative ubiquitin-... 27 1.7
TC86362 similar to GP|10177062|dbj|BAB10504. gene_id:MKP11.2~unk... 27 2.3
TC89824 similar to GP|20465637|gb|AAM20149.1 putative pumilio/Mp... 27 3.0
TC86575 similar to GP|10140758|gb|AAG13589.1 putative ubiquitin ... 27 3.0
BQ750912 similar to GP|7291124|gb| CG15311 gene product {Drosoph... 26 3.9
AW587073 similar to OMNI|SA2279 transporter putative {Staphyloc... 25 6.6
>TC80100 similar to GP|21618014|gb|AAM67064.1 unknown {Arabidopsis
thaliana}, partial (59%)
Length = 740
Score = 264 bits (675), Expect = 6e-72
Identities = 130/130 (100%), Positives = 130/130 (100%)
Frame = +2
Query: 1 MSTLYSPSSAMEDTNHRSKKNQRRRRPSATPTNNDYSDVEDAEDGDPEAWQTLNKSFRQV 60
MSTLYSPSSAMEDTNHRSKKNQRRRRPSATPTNNDYSDVEDAEDGDPEAWQTLNKSFRQV
Sbjct: 83 MSTLYSPSSAMEDTNHRSKKNQRRRRPSATPTNNDYSDVEDAEDGDPEAWQTLNKSFRQV 262
Query: 61 QSVLDRNRAIIQQVNENQQSRMPDNMVKNVGLIQELNGNISKVASLYSDLNSDFTNICHQ 120
QSVLDRNRAIIQQVNENQQSRMPDNMVKNVGLIQELNGNISKVASLYSDLNSDFTNICHQ
Sbjct: 263 QSVLDRNRAIIQQVNENQQSRMPDNMVKNVGLIQELNGNISKVASLYSDLNSDFTNICHQ 442
Query: 121 QQRSSHNRGK 130
QQRSSHNRGK
Sbjct: 443 QQRSSHNRGK 472
>AJ501135 similar to GP|4680489|gb|A hypothetical protein {Oryza sativa}
[Oryza sativa (japonica cultivar-group)], partial (83%)
Length = 615
Score = 85.1 bits (209), Expect = 7e-18
Identities = 38/83 (45%), Positives = 60/83 (71%)
Frame = +2
Query: 33 NNDYSDVEDAEDGDPEAWQTLNKSFRQVQSVLDRNRAIIQQVNENQQSRMPDNMVKNVGL 92
++ +S + + D + T KSF QVQ++LD+NR +I ++N+N +S++PDN+ +NVGL
Sbjct: 269 DDTFSGIGNGTQIDTKILGTFQKSFVQVQNILDQNRLLINEINQNHESKIPDNLSRNVGL 448
Query: 93 IQELNGNISKVASLYSDLNSDFT 115
I+ELN NI +V LY+DL+S FT
Sbjct: 449 IKELNNNIRRVVDLYADLSSSFT 517
>AL388398 similar to GP|8778455|gb|A F1L3.15 {Arabidopsis thaliana}, partial
(68%)
Length = 499
Score = 82.8 bits (203), Expect = 3e-17
Identities = 37/70 (52%), Positives = 53/70 (74%)
Frame = +1
Query: 46 DPEAWQTLNKSFRQVQSVLDRNRAIIQQVNENQQSRMPDNMVKNVGLIQELNGNISKVAS 105
D + QT K F QVQ++LD+ R +I ++N+N +SR+PDN+ KNVGLI+ELN NI +V
Sbjct: 136 DGKILQTFQKRFVQVQNILDQKRVLINEINQNHESRVPDNLNKNVGLIKELNSNIRRVVD 315
Query: 106 LYSDLNSDFT 115
LY+DL++ FT
Sbjct: 316 LYADLSTSFT 345
>TC90077 similar to GP|8778455|gb|AAF79463.1| F1L3.15 {Arabidopsis
thaliana}, partial (64%)
Length = 677
Score = 79.0 bits (193), Expect = 5e-16
Identities = 35/80 (43%), Positives = 52/80 (64%)
Frame = +3
Query: 36 YSDVEDAEDGDPEAWQTLNKSFRQVQSVLDRNRAIIQQVNENQQSRMPDNMVKNVGLIQE 95
+ ++ D D KS Q Q +L++NR +I Q+N+N +SR+PDN+ +NV LI+E
Sbjct: 273 FGELGDTSQVDSRVLHVFQKSLVQAQDILNQNRLLINQINQNHESRIPDNLSRNVSLIKE 452
Query: 96 LNGNISKVASLYSDLNSDFT 115
LN NI +V LY+DL+S FT
Sbjct: 453 LNSNIRRVVDLYADLSSSFT 512
>BF519497 similar to GP|20197919|gb| Expressed protein {Arabidopsis
thaliana}, partial (53%)
Length = 414
Score = 69.7 bits (169), Expect = 3e-13
Identities = 31/55 (56%), Positives = 43/55 (77%)
Frame = +1
Query: 46 DPEAWQTLNKSFRQVQSVLDRNRAIIQQVNENQQSRMPDNMVKNVGLIQELNGNI 100
D + QT K F QVQ++LD+NR +I ++N+N +SR+PDN+ KNVGLI+ELN NI
Sbjct: 247 DGKILQTFQKRFVQVQNILDQNRVLINEINQNHESRVPDNLNKNVGLIKELNSNI 411
>AW773655 similar to SP|Q93WT0|WR31_ Probable WRKY transcription factor 31
(WRKY DNA-binding protein 31). [Mouse-ear cress],
partial (13%)
Length = 619
Score = 33.1 bits (74), Expect = 0.032
Identities = 16/57 (28%), Positives = 32/57 (56%)
Frame = +2
Query: 61 QSVLDRNRAIIQQVNENQQSRMPDNMVKNVGLIQELNGNISKVASLYSDLNSDFTNI 117
QS++D +I Q N+ +++ D + + +G + N K+ + SD+NS +TN+
Sbjct: 17 QSMMDDRTSINAQDNKRAKTQQTDQLQEELGRV---NAENQKLKDMLSDMNSSYTNL 178
>TC88569 homologue to SP|Q9C519|WRK6_ARATH WRKY transcription factor 6 (WRKY
DNA-binding protein 6) (AtWRKY6). [Mouse-ear cress],
partial (21%)
Length = 1319
Score = 33.1 bits (74), Expect = 0.032
Identities = 16/57 (28%), Positives = 32/57 (56%)
Frame = +2
Query: 61 QSVLDRNRAIIQQVNENQQSRMPDNMVKNVGLIQELNGNISKVASLYSDLNSDFTNI 117
QS++D +I Q N+ +++ D + + +G + N K+ + SD+NS +TN+
Sbjct: 431 QSMMDDRTSINAQDNKRAKTQQTDQLQEELGRV---NAENQKLKDMLSDMNSSYTNL 592
>TC87120 similar to SP|P48724|IF5_PHAVU Eukaryotic translation initiation
factor 5 (eIF-5). [Kidney bean French bean] {Phaseolus
vulgaris}, partial (72%)
Length = 1974
Score = 29.6 bits (65), Expect = 0.35
Identities = 23/116 (19%), Positives = 44/116 (37%), Gaps = 3/116 (2%)
Frame = +1
Query: 14 TNHRSKKNQRRRRPSATPTNNDYSDVEDAEDGDPEAWQT---LNKSFRQVQSVLDRNRAI 70
T+ KK +PT + + ++AED D WQT L + ++Q L A
Sbjct: 985 TSSSKKKGNGSDEDHTSPTRSQVDEKDEAEDEDDVQWQTDTSLEAARLRIQEQLSAVTAD 1164
Query: 71 IQQVNENQQSRMPDNMVKNVGLIQELNGNISKVASLYSDLNSDFTNICHQQQRSSH 126
+ ++ + + K NGN +L ++ +D ++ +H
Sbjct: 1165 MVMLSTTEPEKEEKAAAKVSDNPANGNGNSMNYKTLVGEIKADLKKGASAKELVAH 1332
>TC81168 similar to GP|17979303|gb|AAL49877.1 unknown protein {Arabidopsis
thaliana}, partial (34%)
Length = 489
Score = 29.6 bits (65), Expect = 0.35
Identities = 18/89 (20%), Positives = 39/89 (43%), Gaps = 11/89 (12%)
Frame = -3
Query: 53 LNKSFRQVQSVLDRNRAIIQQVNENQQSRMPDNMVKNVGLIQELNGNISKVASLYSDLNS 112
+NK++ ++ +++ Q N++ R+ ++++ +I N IS + + N
Sbjct: 424 VNKNYGKITPQ*HNQQSMKQDQNQDSNKRIKPSIIQRRNIIGNNNSIISNTNNTKNQQNH 245
Query: 113 DFTNI-----------CHQQQRSSHNRGK 130
+ T CH+ RS HN+ K
Sbjct: 244 NRTKSS*SCAELEHVPCHRSSRSMHNQSK 158
>TC91110 similar to GP|7576941|gb|AAF64065.1| putative diacylglycerol
acyltransferase {Brassica napus}, partial (19%)
Length = 692
Score = 29.3 bits (64), Expect = 0.46
Identities = 24/82 (29%), Positives = 35/82 (42%), Gaps = 3/82 (3%)
Frame = +2
Query: 13 DTNHRSKKNQRRRRPSATPTNNDYSDVEDAEDG---DPEAWQTLNKSFRQVQSVLDRNRA 69
+T+ K++ RRRPSAT T D E A D + +LN + V DR
Sbjct: 122 ETDTDLKRSSLRRRPSATSTAGGLFDAESAAADAVRDSGSDDSLNGKINNEEEVKDRKTD 301
Query: 70 IIQQVNENQQSRMPDNMVKNVG 91
+ + ++ DN VK G
Sbjct: 302 HAEGIVDDDD----DNAVKKNG 355
>BQ151193 similar to GP|13359187|dbj KIAA1657 protein {Homo sapiens}, partial
(14%)
Length = 761
Score = 29.3 bits (64), Expect = 0.46
Identities = 21/70 (30%), Positives = 35/70 (50%), Gaps = 4/70 (5%)
Frame = -1
Query: 17 RSKKNQRRRRPSATPTNNDYSDVEDAEDGDPEAWQ----TLNKSFRQVQSVLDRNRAIIQ 72
R KK +R +RP N + E + G E Q T+N++ R+ + R ++I+
Sbjct: 542 RRKKKERDKRPE----ENGKREREKRQGGANETRQKDEGTMNRTHRKKRRQGGRIQSILP 375
Query: 73 QVNENQQSRM 82
Q+NEN + M
Sbjct: 374 QLNENLRQAM 345
>TC78741 weakly similar to GP|21593418|gb|AAM65385.1 unknown {Arabidopsis
thaliana}, partial (68%)
Length = 1522
Score = 28.9 bits (63), Expect = 0.60
Identities = 24/90 (26%), Positives = 38/90 (41%), Gaps = 8/90 (8%)
Frame = +3
Query: 7 PSSAMEDTNHRSKKNQRRRRPSATPT--NNDYSDVEDAEDGDPEAWQTLN------KSFR 58
P +A T+HRSK + +R + PT NN + D D D L
Sbjct: 78 PLNASTATHHRSKVSTFSQRVTPPPTTGNNSNASTSDFNDDDSSFGVLLGTCSYMCPERE 257
Query: 59 QVQSVLDRNRAIIQQVNENQQSRMPDNMVK 88
++Q R+ A+ +++N N + P VK
Sbjct: 258 RIQREKLRDLAVFERLNGNPRKSSPVLAVK 347
>TC76402 weakly similar to GP|18086561|gb|AAL57705.1 At2g01100/F23H14.7
{Arabidopsis thaliana}, partial (43%)
Length = 1449
Score = 28.5 bits (62), Expect = 0.78
Identities = 14/39 (35%), Positives = 21/39 (52%)
Frame = +3
Query: 6 SPSSAMEDTNHRSKKNQRRRRPSATPTNNDYSDVEDAED 44
S S + D ++ K+ RRRRPS ++ YSD + D
Sbjct: 1104 SGSDSYADEVNKRKRRHRRRRPSKFSDSDFYSDEGECSD 1220
>BQ141748 homologue to SP|P21997|SSGP_ Sulfated surface glycoprotein 185 (SSG
185). {Volvox carteri}, partial (3%)
Length = 1293
Score = 27.3 bits (59), Expect = 1.7
Identities = 10/36 (27%), Positives = 23/36 (63%)
Frame = -3
Query: 4 LYSPSSAMEDTNHRSKKNQRRRRPSATPTNNDYSDV 39
++ P+ ++ ++ ++ R+RPS TPT +D ++V
Sbjct: 364 IWPPNKNQKNKIKQNSQSPHRQRPSQTPTRDDITNV 257
>TC77368 similar to GP|14596081|gb|AAK68768.1 putative ubiquitin-like
protein {Arabidopsis thaliana}, partial (73%)
Length = 2125
Score = 27.3 bits (59), Expect = 1.7
Identities = 15/60 (25%), Positives = 29/60 (48%)
Frame = +2
Query: 46 DPEAWQTLNKSFRQVQSVLDRNRAIIQQVNENQQSRMPDNMVKNVGLIQELNGNISKVAS 105
+PE + L S Q+Q ++DRN + +N+ R +N +++E+ N + S
Sbjct: 743 NPEIVRNLLMSNPQMQELMDRNPELAHILNDPSTLRQTLEATRNPEIMREMMRNTDRAMS 922
>TC86362 similar to GP|10177062|dbj|BAB10504. gene_id:MKP11.2~unknown
protein {Arabidopsis thaliana}, partial (70%)
Length = 1113
Score = 26.9 bits (58), Expect = 2.3
Identities = 12/30 (40%), Positives = 17/30 (56%)
Frame = -1
Query: 1 MSTLYSPSSAMEDTNHRSKKNQRRRRPSAT 30
+ T SS+ T+H+S N RR+PS T
Sbjct: 966 LKTKNKQSSSST*TSHKSNNNSNRRQPSTT 877
>TC89824 similar to GP|20465637|gb|AAM20149.1 putative pumilio/Mpt5 family
RNA-binding protein {Arabidopsis thaliana}, partial (4%)
Length = 786
Score = 26.6 bits (57), Expect = 3.0
Identities = 14/61 (22%), Positives = 29/61 (46%)
Frame = -2
Query: 8 SSAMEDTNHRSKKNQRRRRPSATPTNNDYSDVEDAEDGDPEAWQTLNKSFRQVQSVLDRN 67
SS EDT+ + + + + P++ DY+D ++ + P Q + F+ S + +
Sbjct: 686 SSYTEDTSDQQTEPEEHHQQFDFPSDQDYADQKENQLPKPNHLQEFSSPFQGKPSWMQQQ 507
Query: 68 R 68
R
Sbjct: 506 R 504
>TC86575 similar to GP|10140758|gb|AAG13589.1 putative ubiquitin protein
{Oryza sativa}, partial (58%)
Length = 2169
Score = 26.6 bits (57), Expect = 3.0
Identities = 14/60 (23%), Positives = 29/60 (48%)
Frame = +2
Query: 46 DPEAWQTLNKSFRQVQSVLDRNRAIIQQVNENQQSRMPDNMVKNVGLIQELNGNISKVAS 105
+PE + L + Q+Q ++DRN + +N+ R +N +++E+ N + S
Sbjct: 650 NPEIVRNLIMNNPQMQELMDRNPELAHVLNDPSTLRQTLEATRNPEIMREMMRNTDRAMS 829
>BQ750912 similar to GP|7291124|gb| CG15311 gene product {Drosophila
melanogaster}, partial (2%)
Length = 682
Score = 26.2 bits (56), Expect = 3.9
Identities = 12/28 (42%), Positives = 17/28 (59%)
Frame = -2
Query: 7 PSSAMEDTNHRSKKNQRRRRPSATPTNN 34
PSS T+ +++ +RRRR A TNN
Sbjct: 147 PSSTPNSTSASARQRRRRRRRRAGATNN 64
>AW587073 similar to OMNI|SA2279 transporter putative {Staphylococcus aureus
COL}, partial (7%)
Length = 598
Score = 25.4 bits (54), Expect = 6.6
Identities = 16/48 (33%), Positives = 25/48 (51%), Gaps = 4/48 (8%)
Frame = -1
Query: 40 EDAEDGDPEAWQTLNK-SFRQVQSV---LDRNRAIIQQVNENQQSRMP 83
ED + +P + K +F+ Q++ ++ N AIIQ N QQS P
Sbjct: 406 EDFQQSEPNKLELKGK*NFKNQQNIVKHINGNNAIIQFNNHGQQSSKP 263
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.309 0.123 0.340
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 3,285,010
Number of Sequences: 36976
Number of extensions: 31892
Number of successful extensions: 234
Number of sequences better than 10.0: 45
Number of HSP's better than 10.0 without gapping: 232
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 234
length of query: 130
length of database: 9,014,727
effective HSP length: 85
effective length of query: 45
effective length of database: 5,871,767
effective search space: 264229515
effective search space used: 264229515
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.7 bits)
S2: 53 (25.0 bits)
Medicago: description of AC145219.5