Medicago
BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= AC145109.3 - phase: 0 
         (190 letters)

Database: MTGI 
           36,976 sequences; 27,044,181 total letters

Searching..................................................done


                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

TC88971 similar to SP|P92792|OM20_SOLTU Mitochondrial import rec...   138  1e-33
TC93173 weakly similar to SP|P92792|OM20_SOLTU Mitochondrial imp...   103  5e-23
BF004807 similar to PIR|A86217|A862 protein T23G18.18 [imported]...    31  0.25
TC85851 similar to PIR|T12180|T12180 probable transcription fact...    30  0.43
BG453304 homologue to GP|13786464|gb| unknown protein {Oryza sat...    30  0.74
TC85903 similar to PIR|B84530|B84530 probable RING zinc finger p...    28  1.6
AL387278                                                               28  1.6
TC80593 similar to GP|1633130|pdb|1SCH|A Chain A  Peanut Peroxid...    27  3.7
BG585180                                                               26  8.2
TC85798 similar to PIR|T51979|T51979 multicatalytic endopeptidas...    26  8.2
TC87575 weakly similar to GP|7340858|dbj|BAA92948.1 Similar to A...    26  8.2
TC86917 similar to GP|3776080|emb|CAA77093.1 MtN9 {Medicago trun...    26  8.2

>TC88971 similar to SP|P92792|OM20_SOLTU Mitochondrial import receptor
           subunit TOM20 (Translocase of outer membrane 20 kDa
           subunit). [Potato], partial (97%)
          Length = 956

 Score =  138 bits (347), Expect = 1e-33
 Identities = 82/169 (48%), Positives = 101/169 (59%), Gaps = 5/169 (2%)
 Frame = +3

Query: 1   MDYPLRKFKFHLPMKHECILAEQRYNKNPHDAENLTRWGVALLRLSQAHSFPDSLHTIQV 60
           MD+   +F   L  +H    AE  Y KNP DA+NLTRWG ALL LSQ    P+S      
Sbjct: 69  MDFQQNEFDRILFFEHARKTAEAEYAKNPLDADNLTRWGGALLELSQFQPLPESKKMTLD 248

Query: 61  SISKLEEAFSLNPNNPDVHWLLGMALTMQALLTPDSHDAKLHFDSADVYFKRAFRQDPSD 120
           +ISKLEEA S+NPN  D  W LG ALT QA L PD  +AK++FD A VYF++A  +DPS+
Sbjct: 249 AISKLEEALSVNPNKHDALWCLGNALTSQAFLNPDVDEAKVYFDKAAVYFQQAIDEDPSN 428

Query: 121 PTYQISLELPDTKDHEQHPKIVNHGLGQQS-----KGSSSATKVIKHYK 164
             Y+ SLE+   K  E H +I  HGLGQQ+       SS+ TK  K  K
Sbjct: 429 ELYRKSLEVA-AKAPELHVEIHKHGLGQQAVEAAGPSSSAGTKTQKKKK 572


>TC93173 weakly similar to SP|P92792|OM20_SOLTU Mitochondrial import
           receptor subunit TOM20 (Translocase of outer membrane 20
           kDa subunit). [Potato], partial (89%)
          Length = 884

 Score =  103 bits (256), Expect = 5e-23
 Identities = 64/158 (40%), Positives = 88/158 (55%), Gaps = 5/158 (3%)
 Frame = +1

Query: 21  AEQRYNKNPHDAENLTRWGVALLRLSQAHSFPDSLHTIQVSISKLEEAFSLNPNNPDVHW 80
           AE+ Y ++P DA+NLT+WG AL+ LS   +  DS   I+ + +KLEEA  ++P      W
Sbjct: 64  AEENYVQDPLDADNLTKWGEALIELSSCQNPRDSKKMIEDARTKLEEALEIDPTKHYTLW 243

Query: 81  LLGMALTMQALLTPDSHDAKLHFDSADVYFKRAFRQDPSDPTYQISLELPDTKDHEQHPK 140
            LG ALT    LTPD  DAK HFD A  YF++A   DP +  Y+ SL++   +  E H +
Sbjct: 244 CLGNALTSCGFLTPDLSDAKGHFDKAYEYFQKAVDVDPENGLYRQSLKVA-LQAPELHME 420

Query: 141 IVNHGLGQQSKG-----SSSATKVIKHYKFLVFLFTLL 173
           I  +G+GQ   G     +SS  K  K  K   F + LL
Sbjct: 421 IHKNGIGQMGLGGGGASTSSKVKESKKQKSSEFTYDLL 534


>BF004807 similar to PIR|A86217|A862 protein T23G18.18 [imported] -
           Arabidopsis thaliana, partial (15%)
          Length = 521

 Score = 31.2 bits (69), Expect = 0.25
 Identities = 23/67 (34%), Positives = 28/67 (41%)
 Frame = +3

Query: 81  LLGMALTMQALLTPDSHDAKLHFDSADVYFKRAFRQDPSDPTYQISLELPDTKDHEQHPK 140
           LLGM      L  P S+D     D  +  F  + R   SD  +    E   TKD  +H  
Sbjct: 240 LLGMVYQATQLPAPKSNDYDSLVDDEENGFMVSTRIQLSDGRFLAYRESGVTKDKAKHSI 419

Query: 141 IVNHGLG 147
           IV HG G
Sbjct: 420 IVVHGFG 440


>TC85851 similar to PIR|T12180|T12180 probable transcription factor - fava
          bean, partial (93%)
          Length = 2007

 Score = 30.4 bits (67), Expect = 0.43
 Identities = 19/47 (40%), Positives = 26/47 (54%), Gaps = 2/47 (4%)
 Frame = +1

Query: 50 SFPDSLHTIQVSISKLEEAFSL--NPNNPDVHWLLGMALTMQALLTP 94
          S   S HT+ +S+S     FSL  NP+  D++ LL   +TMQA   P
Sbjct: 25 SLQHSTHTLSLSLSHCG*GFSLSLNPSLSDLYLLLRTMVTMQA*FQP 165


>BG453304 homologue to GP|13786464|gb| unknown protein {Oryza sativa},
           partial (2%)
          Length = 657

 Score = 29.6 bits (65), Expect = 0.74
 Identities = 13/47 (27%), Positives = 23/47 (48%)
 Frame = +2

Query: 28  NPHDAENLTRWGVALLRLSQAHSFPDSLHTIQVSISKLEEAFSLNPN 74
           NPH+ + L  WG+ L  L+   +  +    +Q SI +  +A    P+
Sbjct: 437 NPHNPQALNNWGLVLQELATMQAPAEQSALVQQSIWRFRQAIRQRPD 577


>TC85903 similar to PIR|B84530|B84530 probable RING zinc finger protein
            [imported] - Arabidopsis thaliana, partial (26%)
          Length = 2572

 Score = 28.5 bits (62), Expect = 1.6
 Identities = 17/55 (30%), Positives = 30/55 (53%)
 Frame = +2

Query: 136  EQHPKIVNHGLGQQSKGSSSATKVIKHYKFLVFLFTLLVIGYQLPNSQFSCILSW 190
            E  P + N+GLG  ++  S ++ +I  Y+FL F+ +   I Y +  ++   IL W
Sbjct: 2246 ESVPNLQNNGLGLVTRRISPSSHIISIYQFLSFVGS---IYYFVLFNELPLILDW 2401


>AL387278 
          Length = 570

 Score = 28.5 bits (62), Expect = 1.6
 Identities = 11/21 (52%), Positives = 14/21 (66%)
 Frame = +1

Query: 15  KHECILAEQRYNKNPHDAENL 35
           KH C+LA  RY+  PH A +L
Sbjct: 166 KHACLLARTRYSDRPHAAYSL 228


>TC80593 similar to GP|1633130|pdb|1SCH|A Chain A  Peanut Peroxidase,
           partial (68%)
          Length = 1137

 Score = 27.3 bits (59), Expect = 3.7
 Identities = 15/44 (34%), Positives = 21/44 (47%)
 Frame = -1

Query: 139 PKIVNHGLGQQSKGSSSATKVIKHYKFLVFLFTLLVIGYQLPNS 182
           P +   GL       +SA   +K  KFL++L T   +   LPNS
Sbjct: 840 PSVPVSGLSIDILNIASAKSFLKRPKFLLYLLT*AFVDVPLPNS 709


>BG585180 
          Length = 589

 Score = 26.2 bits (56), Expect = 8.2
 Identities = 31/129 (24%), Positives = 50/129 (38%), Gaps = 9/129 (6%)
 Frame = +1

Query: 47  QAHSFPDSLHTIQVSISKLEEAFSLNPNNPDVHWLLGMALTMQALLTPDSHDAKLHFDSA 106
           Q H   D+ H     I KL       P  PD+ +     + +   L  +  + ++  D +
Sbjct: 19  QYHQLRDNNHGKVNDIIKLPSISDTLPK-PDLFYKYNNNIIINNGLMLEEGEEEMSRDQS 195

Query: 107 DVYFKRAFRQDPSD-------PTYQ-ISLELPDTKDHEQHPKIVNHGLGQQSKGSSSATK 158
           +   K+     P+D       P YQ  S    +         I   G G + +G  + TK
Sbjct: 196 EALGKQLCHASPNDIAKMIYQPFYQGSSTNYNNCYGGGTGYNICRCGTGCKCEGCKTHTK 375

Query: 159 -VIKHYKFL 166
            +IKHY+FL
Sbjct: 376 KIIKHYRFL 402


>TC85798 similar to PIR|T51979|T51979 multicatalytic endopeptidase complex
           (EC 3.4.99.46) chain PBB2 [imported] - Arabidopsis
           thaliana, partial (94%)
          Length = 1161

 Score = 26.2 bits (56), Expect = 8.2
 Identities = 10/14 (71%), Positives = 11/14 (78%)
 Frame = +3

Query: 177 YQLPNSQFSCILSW 190
           +QLP S FSCI SW
Sbjct: 486 FQLPRSCFSCISSW 527


>TC87575 weakly similar to GP|7340858|dbj|BAA92948.1 Similar to Arabidopsis
           thaliana chromosome 2 BAC clone F4L23 ; hypothetical
           protein. (AC002387), partial (8%)
          Length = 1188

 Score = 26.2 bits (56), Expect = 8.2
 Identities = 37/157 (23%), Positives = 65/157 (40%)
 Frame = +2

Query: 5   LRKFKFHLPMKHECILAEQRYNKNPHDAENLTRWGVALLRLSQAHSFPDSLHTIQVSISK 64
           L+ FK  + + HE             + E L +  V    LS+  +   S+ TI+ S ++
Sbjct: 530 LKNFKVDINLNHE------------EEDEELKKKVVTEALLSKVFA---SISTIKASYAQ 664

Query: 65  LEEAFSLNPNNPDVHWLLGMALTMQALLTPDSHDAKLHFDSADVYFKRAFRQDPSDPTYQ 124
           L+ A S  P + D     G+    + +++    + K   +    +FK+ F  DPS     
Sbjct: 665 LQHAES--PYDRD-----GIQDADKLIIS----ELKTLAELKQCWFKKQFNFDPSPEVAI 811

Query: 125 ISLELPDTKDHEQHPKIVNHGLGQQSKGSSSATKVIK 161
           ++ EL + K +    KIV   L  +S    S    +K
Sbjct: 812 VAAELKELKSNIITHKIVGKKLESKSLLKDSEIMFLK 922


>TC86917 similar to GP|3776080|emb|CAA77093.1 MtN9 {Medicago truncatula},
           partial (59%)
          Length = 1294

 Score = 26.2 bits (56), Expect = 8.2
 Identities = 15/30 (50%), Positives = 20/30 (66%)
 Frame = +1

Query: 155 SATKVIKHYKFLVFLFTLLVIGYQLPNSQF 184
           S TK++K Y+F  FLF LL+IG    +S F
Sbjct: 91  SLTKMMKLYQFEFFLF-LLIIGNTTLSSSF 177


  Database: MTGI
    Posted date:  Oct 22, 2004  3:39 PM
  Number of letters in database: 27,044,181
  Number of sequences in database:  36,976
  
Lambda     K      H
   0.321    0.136    0.417 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 6,817,162
Number of Sequences: 36976
Number of extensions: 95271
Number of successful extensions: 472
Number of sequences better than 10.0: 25
Number of HSP's better than 10.0 without gapping: 470
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 470
length of query: 190
length of database: 9,014,727
effective HSP length: 91
effective length of query: 99
effective length of database: 5,649,911
effective search space: 559341189
effective search space used: 559341189
frameshift window, decay const: 50,  0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 55 (25.8 bits)


Medicago: description of AC145109.3