BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC145061.27 - phase: 0 /pseudo
(615 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BG452205 weakly similar to GP|1345502|emb TNP2 {Antirrhinum maju... 133 2e-31
BF650639 weakly similar to GP|22748425|g putative transposon pro... 126 3e-29
AI974280 weakly similar to GP|20279447|g putative TNP2-like tran... 117 1e-26
AW257182 weakly similar to GP|20279447|g putative TNP2-like tran... 102 3e-22
BF636730 82 8e-16
BG586318 similar to GP|6069576|dbj| transposon-like ORF {Brassic... 71 1e-12
TC87958 weakly similar to GP|20279447|gb|AAM18727.1 putative TNP... 64 2e-10
TC89338 40 0.003
AJ548089 33 0.25
TC79445 similar to GP|15215626|gb|AAK91358.1 AT4g01560/F11O4_6 {... 33 0.32
AW584704 similar to SP|P05790|FBOH Fibroin heavy chain precursor... 30 3.6
BG646527 30 3.6
TC80593 similar to GP|1633130|pdb|1SCH|A Chain A Peanut Peroxid... 30 3.6
AL389383 28 7.9
>BG452205 weakly similar to GP|1345502|emb TNP2 {Antirrhinum majus}, partial
(16%)
Length = 619
Score = 133 bits (335), Expect = 2e-31
Identities = 69/171 (40%), Positives = 102/171 (59%), Gaps = 5/171 (2%)
Frame = +1
Query: 206 LPLSFYEAQKLVEKLGLEVKTTDCCVNGCMLFYDNEFGKNDGALVACKFCNAPRY-EVCD 264
+P S+ + + ++ LGL+ D C N CML++ + KND ++C C A R+ E
Sbjct: 121 VPDSYNKTR*MIRDLGLDYSKIDACPNDCMLYWKDH--KND---ISCHVCGASRWIESTT 285
Query: 265 DAG----SQKKKRVSIKSMFYLPIIPRLQRLFASTHTADKMTWHYYNKPNSGVMRHPCDG 320
++G ++K+ +VS K + + P+IPRLQRLF + TA + WH+ + G +RHP DG
Sbjct: 286 ESGQIEENKKEHKVSTKILRHFPLIPRLQRLFMCSETATSLRWHHDDCSKDGKLRHPADG 465
Query: 321 VAWKHFDQVHRDFAEDPHNVRLGLCSDGFIPYIQASATPYSCWPILLTPYN 371
AWK F+ H +FA D N+RLGL SDGF P+ +A Y WP+ L PYN
Sbjct: 466 QAWKDFNGHHPEFARDSRNIRLGLASDGFNPF-RAMNLKYXAWPVXLIPYN 615
>BF650639 weakly similar to GP|22748425|g putative transposon protein {Oryza
sativa (japonica cultivar-group)}, partial (6%)
Length = 568
Score = 126 bits (316), Expect = 3e-29
Identities = 66/160 (41%), Positives = 91/160 (56%), Gaps = 5/160 (3%)
Frame = +2
Query: 228 DCCVNGCMLFYDNEFGKNDGALVACKFCNAPRY-EVCDDAG----SQKKKRVSIKSMFYL 282
D C N CML++ + KND ++C C A R E + G ++K+ +VS K + +
Sbjct: 77 DACXNDCMLYWKDH--KND---ISCHVCGASRXIESTTEXGQIEENKKEHKVSTKILRHF 241
Query: 283 PIIPRLQRLFASTHTADKMTWHYYNKPNSGVMRHPCDGVAWKHFDQVHRDFAEDPHNVRL 342
P+IP LQR F + TA + WH+ + G +RHP DG AWK F+ H +FA D N+ L
Sbjct: 242 PLIPXLQRXFMCSETATSLRWHHDDXSKDGKLRHPADGQAWKDFNGHHPEFARDSRNIXL 421
Query: 343 GLCSDGFIPYIQASATPYSCWPILLTPYNVPLEMCMSKPY 382
GL SDGF P+ +A YS WP +L PYN P +CM Y
Sbjct: 422 GLASDGFNPF-RAMNLKYSAWPXILIPYNFPPWLCMKAXY 538
>AI974280 weakly similar to GP|20279447|g putative TNP2-like transposon
protein {Oryza sativa (japonica cultivar-group)},
partial (8%)
Length = 457
Score = 117 bits (293), Expect = 1e-26
Identities = 61/132 (46%), Positives = 81/132 (61%), Gaps = 1/132 (0%)
Frame = +1
Query: 385 LSCIVPGPTSSLDGIDVYIQPLIDDLNRLWN-GVSTYDIARKKIFRMRAALMWTINDFPA 443
LS ++PGP+S IDVY++PLID+L LW G YD + K++F AAL+WTI+D PA
Sbjct: 16 LSLLIPGPSSPKRNIDVYLEPLIDELKELWERGFEVYDASSKQLFEAHAALLWTISDLPA 195
Query: 444 YGMLS*WSTHGRLACPHCMEQTKAIWFHHGRKHS*FDCHRPFLPATHEFRKKKNLFTKGK 503
Y MLS WST G+LACP C T +++ H RK + + HR FL H +RK K F K
Sbjct: 196 YTMLSGWSTSGKLACPVCSYDTCSMYLKHSRK-TCYMSHRRFLDPKHRWRKDKKGFDGKK 372
Query: 504 TEKDGPPPRTTS 515
+ P P + S
Sbjct: 373 EIRVAPFPLSGS 408
>AW257182 weakly similar to GP|20279447|g putative TNP2-like transposon
protein {Oryza sativa (japonica cultivar-group)},
partial (6%)
Length = 387
Score = 102 bits (255), Expect = 3e-22
Identities = 52/102 (50%), Positives = 67/102 (64%), Gaps = 1/102 (0%)
Frame = +1
Query: 407 IDDLNRLW-NGVSTYDIARKKIFRMRAALMWTINDFPAYGMLS*WSTHGRLACPHCMEQT 465
I++L LW +GV TYD ++ ++FRMRAAL+WTI+DFPAY MLS WST G+LACP C T
Sbjct: 7 IEELKVLWESGVETYDASKNQMFRMRAALLWTISDFPAYAMLSGWSTKGKLACPCCNNNT 186
Query: 466 KAIWFHHGRKHS*FDCHRPFLPATHEFRKKKNLFTKGKTEKD 507
+ + H RK D HR FLP H +R+ + F GK D
Sbjct: 187 SSTYLKHSRKMCYMD-HRTFLPMDHAWRENRKSF-NGKKNLD 306
>BF636730
Length = 637
Score = 81.6 bits (200), Expect = 8e-16
Identities = 39/86 (45%), Positives = 50/86 (57%)
Frame = +1
Query: 7 HRSWMYDRKYPGKRKLKAAFVDGVRDFVAYAMAQDAFQLEGGIRCSCVKCTCRLIRSPKD 66
HR WM +R P ++ F+ GV +FV +A AQD F+ G +RC C C CR S D
Sbjct: 298 HRKWMDNRMRPDNSRVTDEFLAGVCEFVEFACAQDDFKKVGKLRCPCKICKCRKP*SVDD 477
Query: 67 VLNHLKDLGFMENYYVWIYHGEQEPT 92
V+ HL GF E+YY W HGEQ P+
Sbjct: 478 VMKHLCMKGFKEDYYYWSSHGEQRPS 555
>BG586318 similar to GP|6069576|dbj| transposon-like ORF {Brassica rapa},
partial (11%)
Length = 267
Score = 70.9 bits (172), Expect = 1e-12
Identities = 31/56 (55%), Positives = 41/56 (72%)
Frame = +3
Query: 560 WKDNLLRHNFDVMHIEKNFCDNIIHIVMNVSAKTKDNEKARMDLPLHCVPTSTQED 615
W+D+LLRHN DVMHIEKNF DN+++ ++NV KTKDN K+R+DL C + D
Sbjct: 3 WEDHLLRHNLDVMHIEKNFFDNLMNTILNVQGKTKDNLKSRLDLVDICARSELHVD 170
>TC87958 weakly similar to GP|20279447|gb|AAM18727.1 putative TNP2-like
transposon protein {Oryza sativa (japonica
cultivar-group)}, partial (10%)
Length = 2433
Score = 63.5 bits (153), Expect = 2e-10
Identities = 27/48 (56%), Positives = 34/48 (70%)
Frame = +1
Query: 556 DLPYWKDNLLRHNFDVMHIEKNFCDNIIHIVMNVSAKTKDNEKARMDL 603
+LPYW N L HN DVMHIEKN DNII ++++ KTKD+ + R DL
Sbjct: 1 ELPYWASNTLHHNLDVMHIEKNIFDNIIGTLLDIPGKTKDHVRGRYDL 144
>TC89338
Length = 1088
Score = 39.7 bits (91), Expect = 0.003
Identities = 24/74 (32%), Positives = 40/74 (53%), Gaps = 1/74 (1%)
Frame = +3
Query: 21 KLKAAFVDGVRDFVAYAMAQDAFQLEGGIRCSCVKCTCRLIR-SPKDVLNHLKDLGFMEN 79
+L A + +GV +F+ +A ++ EG C CV C R + S +++ HL +G +N
Sbjct: 243 RLSAEYDNGVTEFLQFA-EKNLPNTEGLFPCPCVSCGNRDPKLSKEEIKGHLVSVGICQN 419
Query: 80 YYVWIYHGEQEPTN 93
Y I+HGE T+
Sbjct: 420 YTQ*IWHGETMMTS 461
Score = 37.7 bits (86), Expect = 0.013
Identities = 20/67 (29%), Positives = 35/67 (51%), Gaps = 1/67 (1%)
Frame = +3
Query: 28 DGVRDFVAYAMAQDAFQLEGGIRCSCVKC-TCRLIRSPKDVLNHLKDLGFMENYYVWIYH 86
+G+ +F+ +A ++ EG C C+ C S +++ HL +G +NY WI+H
Sbjct: 507 NGMTEFLQFA-EKNLPNTEGLFPCPCISCGNWDPKLSKEEIRGHLVSVGICQNYTEWIWH 683
Query: 87 GEQEPTN 93
GE T+
Sbjct: 684 GETMMTS 704
>AJ548089
Length = 560
Score = 33.5 bits (75), Expect = 0.25
Identities = 16/49 (32%), Positives = 25/49 (50%), Gaps = 1/49 (2%)
Frame = +1
Query: 46 EGGIRCSCVKC-TCRLIRSPKDVLNHLKDLGFMENYYVWIYHGEQEPTN 93
EG C C+ C S +++ HL +G +NY WI++GE T+
Sbjct: 226 EGLFPCPCISCGNWDPKLSKEEIRGHLVSVGICQNYTEWIWYGETMMTS 372
>TC79445 similar to GP|15215626|gb|AAK91358.1 AT4g01560/F11O4_6 {Arabidopsis
thaliana}, partial (86%)
Length = 1443
Score = 33.1 bits (74), Expect = 0.32
Identities = 23/89 (25%), Positives = 41/89 (45%), Gaps = 3/89 (3%)
Frame = +3
Query: 466 KAIWFHHGRKHS*FDCHRPFLPATHEFRKKKNLFTKGKTEKDGPPPRTTSEEVYRRVNEL 525
+ + FH+ R F HR E ++ K + +KGK ++DG + + R+ E
Sbjct: 723 RVVTFHNQRDFIFFRHHRYIF----ETKEIKKIESKGKNDEDGKSEKVPDHKTIARLQEC 890
Query: 526 GLLKFTDVGKRVRHPKY---GNEHHWTKR 551
G +FT K ++H + G E+ W +
Sbjct: 891 G-PRFTLKLKSLQHGTFDTKGGEYEWVHK 974
>AW584704 similar to SP|P05790|FBOH Fibroin heavy chain precursor (Fib-H)
(H-fibroin). [Silk moth] {Bombyx mori}, partial (0%)
Length = 679
Score = 29.6 bits (65), Expect = 3.6
Identities = 15/49 (30%), Positives = 27/49 (54%), Gaps = 1/49 (2%)
Frame = -2
Query: 49 IRCSCVKCTCRLIRSPKDVLNHLKDLGFMENYYVWIY-HGEQEPTNNTE 96
+ C CV T L+ SP V+N F+ NY ++ + HG ++ +N ++
Sbjct: 225 LECECVSETKDLVCSPSLVINPKCPTTFISNYKIFCHSHG*EDSSNESK 79
>BG646527
Length = 780
Score = 29.6 bits (65), Expect = 3.6
Identities = 13/38 (34%), Positives = 18/38 (47%), Gaps = 1/38 (2%)
Frame = -3
Query: 296 HTADKMTWHYYNK-PNSGVMRHPCDGVAWKHFDQVHRD 332
H + KMTWH+Y K P++ + H D RD
Sbjct: 706 HVSPKMTWHFYLKIPSNSCFLGHISAIRSHHLDHASRD 593
>TC80593 similar to GP|1633130|pdb|1SCH|A Chain A Peanut Peroxidase,
partial (68%)
Length = 1137
Score = 29.6 bits (65), Expect = 3.6
Identities = 34/134 (25%), Positives = 56/134 (41%), Gaps = 6/134 (4%)
Frame = +2
Query: 133 NEGGEEETIPNEKALKFYKMMQEVNKPLFEGSSDSKLSMSVRLLAAASDWSVAEEGSECY 192
N GE+ PN +L+ ++++ ++ L E + +S S L AA D VAE G + +
Sbjct: 161 NFTGEKNAFPNANSLRGFELIDDIKSQL-EDMCPNTVSCSDILALAARD-GVAELGGQRW 334
Query: 193 TDIM--RDTTPVK----DNLPLSFYEAQKLVEKLGLEVKTTDCCVNGCMLFYDNEFGKND 246
++ RD+T + LP F L+ + T + V G +
Sbjct: 335 NVLLGRRDSTTANLSEANTLPAPFLNLDGLITAFAKKGFTAEEMV--------TLSGAHT 490
Query: 247 GALVACKFCNAPRY 260
LV C+F A Y
Sbjct: 491 IGLVRCRFFRARIY 532
>AL389383
Length = 484
Score = 28.5 bits (62), Expect = 7.9
Identities = 28/113 (24%), Positives = 49/113 (42%), Gaps = 8/113 (7%)
Frame = +3
Query: 115 FGVMEDMVGDAVGVNLSYNEGGEEETIPNEKALKFYKMMQEVNKP----LFEGSSDSKLS 170
FG++ G G+ +N EEE + K L Y++ Q+V P + SS K+S
Sbjct: 141 FGILGLNTGFHGGIKNEFNSTHEEEYKKSVKELVSYRIRQKVEAPPLTSIMVNSSVDKIS 320
Query: 171 MSVRLLAAASDWSVAEEGSECYTDIMR----DTTPVKDNLPLSFYEAQKLVEK 219
+ + A A+ E Y I+ D ++ L +Y+A+ + E+
Sbjct: 321 IDIPFKAKIRVTGKADRLDE-YGRIVPMADVDANALRYYLQKEYYDAKVVAEE 476
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.324 0.138 0.450
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 23,526,579
Number of Sequences: 36976
Number of extensions: 391911
Number of successful extensions: 1805
Number of sequences better than 10.0: 28
Number of HSP's better than 10.0 without gapping: 1789
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1800
length of query: 615
length of database: 9,014,727
effective HSP length: 102
effective length of query: 513
effective length of database: 5,243,175
effective search space: 2689748775
effective search space used: 2689748775
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (22.0 bits)
S2: 61 (28.1 bits)
Medicago: description of AC145061.27