BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC144929.2 + phase: 0
(120 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
CA919100 homologue to PIR|G90291|G902 endoglucanase precursor [i... 117 1e-27
AW686588 101 8e-23
TC80819 weakly similar to GP|10140689|gb|AAG13524.1 putative non... 100 2e-22
TC82520 48 7e-15
BF650593 66 4e-12
BE999296 59 4e-10
BF520135 59 6e-10
BE941032 57 1e-09
BQ124492 weakly similar to GP|9909168|dbj| putative transposable... 43 2e-05
BG456581 40 2e-04
BG586862 40 3e-04
TC77455 similar to GP|22335695|dbj|BAC10549. nine-cis-epoxycarot... 37 0.001
TC83226 weakly similar to PIR|G86419|G86419 probable reverse tra... 35 0.005
TC93136 31 0.13
TC81859 similar to GP|18491135|gb|AAL69536.1 At1g04290/F19P19_27... 30 0.16
BG586749 weakly similar to GP|18138053|emb tropinone reductase I... 29 0.48
BG587113 weakly similar to PIR|A84888|A8 hypothetical protein At... 28 1.1
TC78883 similar to GP|8843731|dbj|BAA97279.1 zinc finger protein... 27 1.4
TC87823 similar to GP|9295275|gb|AAF86907.1| phosphoenolpyruvate... 27 1.8
TC80853 similar to GP|17064740|gb|AAL32524.1 ATP-dependent RNA h... 27 2.4
>CA919100 homologue to PIR|G90291|G902 endoglucanase precursor [imported] -
Sulfolobus solfataricus, partial (3%)
Length = 789
Score = 117 bits (292), Expect = 1e-27
Identities = 54/102 (52%), Positives = 68/102 (65%)
Frame = -2
Query: 17 IWHKQVPLKVSILAWRLIRNRLLMKDNLMVRGTLSHDNQLCVTGCGGLEIAQHLFLSCPC 76
IWH+QVPLKVS+ AWRL+R+RL K NL+ RG + + LCV+GCG LE AQHLFLSC
Sbjct: 584 IWHRQVPLKVSVFAWRLLRDRLPTKSNLIYRGVIPTEAGLCVSGCGALESAQHLFLSCSY 405
Query: 77 FNSQWDLVRSWLDICSADPYYLQKHDIQFAYLSGSLQARRSF 118
F S W LVR W+ D L H +QF + +G +A +SF
Sbjct: 404 FASLWSLVRDWIGFVGVDTNVLSDHFVQFVHSTGGNKASQSF 279
>AW686588
Length = 567
Score = 101 bits (251), Expect = 8e-23
Identities = 51/97 (52%), Positives = 63/97 (64%)
Frame = +1
Query: 22 VPLKVSILAWRLIRNRLLMKDNLMVRGTLSHDNQLCVTGCGGLEIAQHLFLSCPCFNSQW 81
VPLKVSILAWRLIR+RL K NL+ R L+ + CV GCG E A HLFL C F + W
Sbjct: 142 VPLKVSILAWRLIRDRLPTKANLVRRRCLAVEAAGCVVGCGIAETANHLFLHCATFGAVW 321
Query: 82 DLVRSWLDICSADPYYLQKHDIQFAYLSGSLQARRSF 118
+R+W+ + ADP+ L H IQF +G +ARRSF
Sbjct: 322 QHIRAWIGVSGADPHDLSDHFIQFITCTGHTRARRSF 432
>TC80819 weakly similar to GP|10140689|gb|AAG13524.1 putative non-LTR
retroelement reverse transcriptase {Oryza sativa
(japonica cultivar-group)}, partial (2%)
Length = 1262
Score = 100 bits (248), Expect = 2e-22
Identities = 50/103 (48%), Positives = 60/103 (57%)
Frame = +2
Query: 17 IWHKQVPLKVSILAWRLIRNRLLMKDNLMVRGTLSHDNQLCVTGCGGLEIAQHLFLSCPC 76
+WHK +P KVS+ WRL+RNRL KDNL+ RG L N CV GC E HLFL C
Sbjct: 626 VWHKHIPSKVSLFVWRLLRNRLPTKDNLVHRGVLLATNAACVCGCVDSESTTHLFLHCNV 805
Query: 77 FNSQWDLVRSWLDICSADPYYLQKHDIQFAYLSGSLQARRSFF 119
F S W LVR+WL I S L+ H IQFA + G + +F
Sbjct: 806 FCSLWSLVRNWLGIPSMSSGELRTHFIQFAKMVGMPRVSHLYF 934
>TC82520
Length = 833
Score = 47.8 bits (112), Expect(2) = 7e-15
Identities = 24/59 (40%), Positives = 31/59 (51%)
Frame = +2
Query: 60 GCGGLEIAQHLFLSCPCFNSQWDLVRSWLDICSADPYYLQKHDIQFAYLSGSLQARRSF 118
GCG E A HLFL C F S W V WL + P +++ IQF ++GS + SF
Sbjct: 314 GCGDSETATHLFLHCDIFGSLWSHVLRWLHLLLVLPADIRQFFIQFTSMAGSPRFTHSF 490
Score = 47.4 bits (111), Expect(2) = 7e-15
Identities = 20/44 (45%), Positives = 26/44 (58%)
Frame = +3
Query: 17 IWHKQVPLKVSILAWRLIRNRLLMKDNLMVRGTLSHDNQLCVTG 60
+W K +P KVS+ WRL NRL K NLM R L + C++G
Sbjct: 186 VWQKNIPSKVSMFVWRLFHNRLPTKVNLMQRHVLQQTHTACISG 317
>BF650593
Length = 486
Score = 65.9 bits (159), Expect = 4e-12
Identities = 31/63 (49%), Positives = 36/63 (56%)
Frame = +3
Query: 19 HKQVPLKVSILAWRLIRNRLLMKDNLMVRGTLSHDNQLCVTGCGGLEIAQHLFLSCPCFN 78
HK VPLKVS L WRL +N L +DNL RG L ++ CV CG E H F CP F+
Sbjct: 297 HKSVPLKVSCLVWRLFQNXLATRDNLSKRGVLDQNSIXCVXDCGREESVSHFFFECP-FS 473
Query: 79 SQW 81
W
Sbjct: 474 XVW 482
>BE999296
Length = 384
Score = 58.9 bits (141), Expect = 4e-10
Identities = 32/88 (36%), Positives = 42/88 (47%)
Frame = -1
Query: 17 IWHKQVPLKVSILAWRLIRNRLLMKDNLMVRGTLSHDNQLCVTGCGGLEIAQHLFLSCPC 76
+WH +PLKV R++RN L KDN + R + ++ LC TGC E LFL
Sbjct: 240 VWHNHIPLKVCFFVLRVLRNCLPTKDNFVRRRVIHEEHMLCPTGCSFKETTDDLFL---- 73
Query: 77 FNSQWDLVRSWLDICSADPYYLQKHDIQ 104
W LV WL I + L+ H Q
Sbjct: 72 ----WPLV*QWLHIS*VNSCVLRDHFYQ 1
>BF520135
Length = 202
Score = 58.5 bits (140), Expect = 6e-10
Identities = 28/58 (48%), Positives = 34/58 (58%)
Frame = +3
Query: 17 IWHKQVPLKVSILAWRLIRNRLLMKDNLMVRGTLSHDNQLCVTGCGGLEIAQHLFLSC 74
+W K+ KVSI AWRL RL K N+ RG + HD +CVT C +E HLFL C
Sbjct: 15 LWRKEDLSKVSIFAWRLFHGRLPTKANVFKRGIVHHDAHMCVTRCRLIESDVHLFLHC 188
>BE941032
Length = 435
Score = 57.4 bits (137), Expect = 1e-09
Identities = 32/84 (38%), Positives = 37/84 (43%)
Frame = +2
Query: 27 SILAWRLIRNRLLMKDNLMVRGTLSHDNQLCVTGCGGLEIAQHLFLSCPCFNSQWDLVRS 86
SI W ++ NR KDNL+ RG +S Q CV CG L A HLFL C F W V
Sbjct: 146 SIFLWCVLLNRFPTKDNLLKRGVISAIYQSCVGECGNLYDATHLFLHCNFFRQIWINVSD 325
Query: 87 WLDICSADPYYLQKHDIQFAYLSG 110
WL + QF G
Sbjct: 326 WLSFVMVTLLRISDRLAQFGSFGG 397
>BQ124492 weakly similar to GP|9909168|dbj| putative transposable element
Tip100 protein {Oryza sativa (japonica cultivar-group)},
partial (8%)
Length = 694
Score = 43.1 bits (100), Expect = 2e-05
Identities = 20/47 (42%), Positives = 27/47 (56%)
Frame = +2
Query: 64 LEIAQHLFLSCPCFNSQWDLVRSWLDICSADPYYLQKHDIQFAYLSG 110
++ A+HLFL C F+S W V WL I S P L+ H QF ++G
Sbjct: 527 IKTARHLFLDCDIFSSLWSQVWLWLGISSVQPGELRHHFPQFTNMAG 667
>BG456581
Length = 683
Score = 40.4 bits (93), Expect = 2e-04
Identities = 25/72 (34%), Positives = 33/72 (45%)
Frame = +2
Query: 17 IWHKQVPLKVSILAWRLIRNRLLMKDNLMVRGTLSHDNQLCVTGCGGLEIAQHLFLSCPC 76
IW+ +P S + WRL +RL DNL RG +C +E + HLFL C
Sbjct: 80 IWNSCIPPSHSFICWRLAHDRLPTDDNLSSRGCAL--VSMCSFCLEQVETSDHLFLRCKF 253
Query: 77 FNSQWDLVRSWL 88
+ W SWL
Sbjct: 254 VVTLW----SWL 277
>BG586862
Length = 804
Score = 39.7 bits (91), Expect = 3e-04
Identities = 24/62 (38%), Positives = 31/62 (49%)
Frame = -1
Query: 20 KQVPLKVSILAWRLIRNRLLMKDNLMVRGTLSHDNQLCVTGCGGLEIAQHLFLSCPCFNS 79
K +P S L WRL+ N L +KD L RG + LC +E QHLFL+C
Sbjct: 642 KTIPRHKSFL-WRLLHNALPVKDELHKRGIRC--SLLCPRCESKIETVQHLFLNCEVTQK 472
Query: 80 QW 81
+W
Sbjct: 471 EW 466
>TC77455 similar to GP|22335695|dbj|BAC10549. nine-cis-epoxycarotenoid
dioxygenase1 {Pisum sativum}, partial (43%)
Length = 1865
Score = 37.4 bits (85), Expect = 0.001
Identities = 29/89 (32%), Positives = 38/89 (42%), Gaps = 2/89 (2%)
Frame = -2
Query: 17 IWHKQVPLKVSILAWRLIRNRLLMKDNLMVRGTLS-HDNQLCV-TGCGGLEIAQHLFLSC 74
+W + P KV +W L +R+ NL R L D++ CV GC E HLFL C
Sbjct: 322 LWKSKAPAKVLAFSWTLFLDRIPTMVNLGKRRLLRVEDSKRCVFCGCQD-ETVVHLFLHC 146
Query: 75 PCFNSQWDLVRSWLDICSADPYYLQKHDI 103
+ V WL+ P L H I
Sbjct: 145 DVISKV*REVMRWLNFNLISPPNLLIHAI 59
>TC83226 weakly similar to PIR|G86419|G86419 probable reverse transcriptase
100033-105622 [imported] - Arabidopsis thaliana, partial
(2%)
Length = 885
Score = 35.4 bits (80), Expect = 0.005
Identities = 19/65 (29%), Positives = 29/65 (44%)
Frame = +3
Query: 17 IWHKQVPLKVSILAWRLIRNRLLMKDNLMVRGTLSHDNQLCVTGCGGLEIAQHLFLSCPC 76
IW + +L WR++ + L ++ +L RG + LC E HLF+SCP
Sbjct: 129 IWSLHTIPRHKVLLWRILNDSLPVRSSLRKRGIQCYP--LCPRCHSKTETITHLFMSCPL 302
Query: 77 FNSQW 81
W
Sbjct: 303 SKRVW 317
>TC93136
Length = 722
Score = 30.8 bits (68), Expect = 0.13
Identities = 11/24 (45%), Positives = 14/24 (57%)
Frame = +1
Query: 65 EIAQHLFLSCPCFNSQWDLVRSWL 88
E + HLFL CP +S W + WL
Sbjct: 262 ETSSHLFLHCPFLSSVWSKILGWL 333
>TC81859 similar to GP|18491135|gb|AAL69536.1 At1g04290/F19P19_27
{Arabidopsis thaliana}, partial (65%)
Length = 960
Score = 30.4 bits (67), Expect = 0.16
Identities = 17/46 (36%), Positives = 23/46 (49%)
Frame = +2
Query: 36 NRLLMKDNLMVRGTLSHDNQLCVTGCGGLEIAQHLFLSCPCFNSQW 81
+RL K N++ R + D+ CV G E +Q LF CP F W
Sbjct: 617 DRLPTKYNVLPREINNLDSSFCVGGWEVNETSQSLFF*CPLF*QGW 754
>BG586749 weakly similar to GP|18138053|emb tropinone reductase I {Calystegia
sepium}, partial (11%)
Length = 787
Score = 28.9 bits (63), Expect = 0.48
Identities = 20/62 (32%), Positives = 30/62 (48%)
Frame = -2
Query: 57 CVTGCGGLEIAQHLFLSCPCFNSQWDLVRSWLDICSADPYYLQKHDIQFAYLSGSLQARR 116
CV G E+++HLFL C DL+R WL P L H + +SG+ + R+
Sbjct: 531 CVLCEGVPEVSEHLFLQCATAKVWADLLR-WLGFDYHTPPDLFFHWKWWNEMSGNKKTRK 355
Query: 117 SF 118
+
Sbjct: 354 GY 349
>BG587113 weakly similar to PIR|A84888|A8 hypothetical protein At2g45230
[imported] - Arabidopsis thaliana, partial (10%)
Length = 767
Score = 27.7 bits (60), Expect = 1.1
Identities = 21/79 (26%), Positives = 30/79 (37%)
Frame = -3
Query: 3 TNEKPAIADVTTNFIWHKQVPLKVSILAWRLIRNRLLMKDNLMVRGTLSHDNQLCVTGCG 62
T ++P++ D+ +W KV WR I N L N+ R +S D G
Sbjct: 327 TVDQPSLDDLYQR-VWKYNTSPKVRHFLWRCISNSLPTAANMRSR-HISKDGSCSRCGME 154
Query: 63 GLEIAQHLFLSCPCFNSQW 81
E H+ CP W
Sbjct: 153 S-ETVNHILFQCPYARLIW 100
>TC78883 similar to GP|8843731|dbj|BAA97279.1 zinc finger protein {Arabidopsis
thaliana}, partial (28%)
Length = 1674
Score = 27.3 bits (59), Expect = 1.4
Identities = 25/81 (30%), Positives = 37/81 (44%), Gaps = 8/81 (9%)
Frame = +1
Query: 15 NFIWHKQVPLKVSILAWRLI--RNRLLMKDNLMVRG----TLSHDNQLCV--TGCGGLEI 66
NF KQV K W L+ ++ ++ L ++G TL+ N L + TGC L +
Sbjct: 1207 NFGSMKQVMCK-----WGLVDLSCTIVPEERLYIKGKENVTLTRYNVLVLDNTGCEKLRV 1371
Query: 67 AQHLFLSCPCFNSQWDLVRSW 87
+ C F QW + RSW
Sbjct: 1372 CAY---GCLKFKGQWSI*RSW 1425
>TC87823 similar to GP|9295275|gb|AAF86907.1| phosphoenolpyruvate/phosphate
translocator precursor {Mesembryanthemum crystallinum},
partial (74%)
Length = 1552
Score = 26.9 bits (58), Expect = 1.8
Identities = 13/26 (50%), Positives = 14/26 (53%), Gaps = 3/26 (11%)
Frame = +2
Query: 55 QLCVTGCGGLEIAQHL---FLSCPCF 77
QLC CG E+ HL LSC CF
Sbjct: 1148 QLCEACCGHCELCYHLQNTCLSCECF 1225
>TC80853 similar to GP|17064740|gb|AAL32524.1 ATP-dependent RNA
helicase-like protein {Arabidopsis thaliana}, partial
(71%)
Length = 1065
Score = 26.6 bits (57), Expect = 2.4
Identities = 10/27 (37%), Positives = 17/27 (62%)
Frame = -1
Query: 48 GTLSHDNQLCVTGCGGLEIAQHLFLSC 74
G ++ +N +C+T GG+ I +FL C
Sbjct: 156 GKVALNNIVCLTPAGGMSICSTIFLIC 76
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.328 0.139 0.459
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 5,012,585
Number of Sequences: 36976
Number of extensions: 81944
Number of successful extensions: 574
Number of sequences better than 10.0: 59
Number of HSP's better than 10.0 without gapping: 570
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 573
length of query: 120
length of database: 9,014,727
effective HSP length: 84
effective length of query: 36
effective length of database: 5,908,743
effective search space: 212714748
effective search space used: 212714748
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.8 bits)
S2: 52 (24.6 bits)
Medicago: description of AC144929.2