BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC144893.1 + phase: 0 /pseudo
(311 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BG454068 weakly similar to GP|22202804|dbj hypothetical protein~... 99 3e-21
BG453710 weakly similar to GP|21537057|gb| unknown {Arabidopsis ... 63 8e-18
AW693426 homologue to GP|23307580|dbj similar to serine/threonin... 74 6e-14
TC83089 47 9e-06
CA917035 similar to GP|7303305|gb|A CG6061-PA {Drosophila melano... 44 8e-05
BG646599 40 0.001
TC77848 similar to PIR|F86296|F86296 hypothetical protein AAF185... 31 0.53
TC76797 homologue to GP|8954298|gb|AAD27882.2| chlorophyll a/b-b... 30 0.91
TC82474 30 1.2
BF633515 30 1.2
TC88489 similar to GP|14334978|gb|AAK59666.1 unknown protein {Ar... 29 2.7
TC86205 similar to PIR|A86289|A86289 probable ABC transporter [i... 28 5.9
CA920886 similar to GP|11994399|db contains similarity to recept... 27 7.7
BG583982 27 7.7
TC85062 similar to EGAD|119543|127780 hypothetical protein F49C5... 27 7.7
TC86206 similar to GP|20522008|dbj|BAB92011. pleiotropic drug re... 27 7.7
>BG454068 weakly similar to GP|22202804|dbj hypothetical protein~predicted by
GeneMark.hmm etc. {Oryza sativa (japonica
cultivar-group)}, partial (5%)
Length = 673
Score = 98.6 bits (244), Expect = 3e-21
Identities = 51/140 (36%), Positives = 76/140 (53%)
Frame = +3
Query: 9 IIAARWWENRLHGTDLGWLQDTSYIMIDHGLIYAFVEMWHEETSSFHLPFEEITVTLDDV 68
+ A W+ N + + L L +T+Y +DHGL+ AF WH ETSSFHLP E+T+ LDD+
Sbjct: 246 VAAMGWFWNVIRASGLYPLLETNYGQVDHGLLIAFSLRWHSETSSFHLPVGEMTIALDDI 425
Query: 69 TYLLHIPIDDMLLSHGFISRDEAVELMEEYLESDTGDALIEVDKTKGAHCRFSYLEKIFK 128
+ LLHIP+ LL H +S + E + YL + + E + K AH + L I+
Sbjct: 426 SCLLHIPVGGNLLFHESLSIHQGTEYLVNYLGLEFEEIAAETKRLKSAHITYDTLLSIYT 605
Query: 129 ECLKEQRDLAAEYGVTEEVE 148
L E + A + G + +E
Sbjct: 606 SYLTEAKSYANQPGEEDSME 665
>BG453710 weakly similar to GP|21537057|gb| unknown {Arabidopsis thaliana},
partial (6%)
Length = 651
Score = 62.8 bits (151), Expect(2) = 8e-18
Identities = 29/48 (60%), Positives = 33/48 (68%)
Frame = -1
Query: 36 DHGLIYAFVEMWHEETSSFHLPFEEITVTLDDVTYLLHIPIDDMLLSH 83
D +I A E WH ETSSFHLP E+T+TLDDV LLHIPI +L H
Sbjct: 474 DPSMIRAMCERWHTETSSFHLPMGEMTITLDDVYNLLHIPIHGCMLDH 331
Score = 44.7 bits (104), Expect(2) = 8e-18
Identities = 28/102 (27%), Positives = 50/102 (48%), Gaps = 2/102 (1%)
Frame = -3
Query: 91 AVELMEEYLESDTGDALIEVDKTKGAHCRFSYLEKIFKECLKEQRDLAAEYGVTEEVERL 150
A++LM L +A+ E+ H + L++++++ L E R L E ER
Sbjct: 307 AIDLMTRLLGMSDANAMAEIRTESAGHISYPTLKRVYEDHLIEARRLEDPLTREELQERA 128
Query: 151 QDQT--VRIYLLYLVGITIFTDKSQWAVDVVYLKFFRNIDLV 190
+ + VR LLYLV +FT K+ +D++YL ++ +
Sbjct: 127 RRRQWCVRSLLLYLVDCVLFTYKTNRHIDLIYLDCMADLQAI 2
>AW693426 homologue to GP|23307580|dbj similar to serine/threonine
phosphatase PP7 {Oryza sativa (japonica
cultivar-group)}, partial (2%)
Length = 209
Score = 74.3 bits (181), Expect = 6e-14
Identities = 35/67 (52%), Positives = 49/67 (72%)
Frame = +1
Query: 158 YLLYLVGITIFTDKSQWAVDVVYLKFFRNIDLVSSFSWGVAALAHLY*ELNNAARWNCSQ 217
+LL LVG T+F+DKS + V+V YL+FFR++D ++WGVAALAHLY L A+ +
Sbjct: 1 FLLCLVGCTLFSDKSTFVVNVAYLEFFRDLDSCGGYAWGVAALAHLYDNLRYASFHHMKS 180
Query: 218 MAGYLTL 224
++GYLTL
Sbjct: 181 ISGYLTL 201
>TC83089
Length = 887
Score = 47.0 bits (110), Expect = 9e-06
Identities = 29/91 (31%), Positives = 49/91 (52%), Gaps = 1/91 (1%)
Frame = +3
Query: 142 GVTEEVERLQDQTVRIYLLYLVGITIFTDKSQWAVDVVYLKFFRN-IDLVSSFSWGVAAL 200
G +E+E+++ V+ YLLYLVG +F DKS +++VYL + + + SWG L
Sbjct: 327 GDPQELEKIRTTCVKWYLLYLVGCLLFGDKSNKRIELVYLTTMEDGYAGMRNHSWGGMTL 506
Query: 201 AHLY*ELNNAARWNCSQMAGYLTLFVYGLYI 231
A+LY L+ + + +TL G ++
Sbjct: 507 AYLYHCLSEVSLPGGGALERSVTLVTAGWFL 599
>CA917035 similar to GP|7303305|gb|A CG6061-PA {Drosophila melanogaster},
partial (2%)
Length = 365
Score = 43.9 bits (102), Expect = 8e-05
Identities = 20/43 (46%), Positives = 28/43 (64%)
Frame = +2
Query: 13 RWWENRLHGTDLGWLQDTSYIMIDHGLIYAFVEMWHEETSSFH 55
R+W N + + L L +T+Y +DH L+ AF + WH ETSSFH
Sbjct: 239 RFW-NVIMASGLYLLLETNYGQVDHSLLIAFSDRWHSETSSFH 364
>BG646599
Length = 711
Score = 40.0 bits (92), Expect = 0.001
Identities = 30/109 (27%), Positives = 45/109 (40%), Gaps = 5/109 (4%)
Frame = +3
Query: 207 LNNAARWNCSQMAGYLTLFVYGLYIYTWLSLVQAWVFHHFPGMGSNEVWENYVENQYP-- 264
L N + +C Q+ GY T L+Q W++ +FP +G+ EN V P
Sbjct: 6 LGNGSMMSCKQLGGYAT-------------LLQCWIYEYFPTLGNRV--ENRVACDEPGM 140
Query: 265 ---RAMLFLPLSGLGTVDHYINYLDALDLTRVVMAQYGVHRQARQFEQV 310
RAM + G VD +D L V+ + HRQ F+ +
Sbjct: 141 GVARAMRWKYPRGNLKVDQIRRMIDDLTPNDVIWCPFDSHRQVIPFDDI 287
>TC77848 similar to PIR|F86296|F86296 hypothetical protein AAF18512.1
[imported] - Arabidopsis thaliana, complete
Length = 1592
Score = 31.2 bits (69), Expect = 0.53
Identities = 22/89 (24%), Positives = 37/89 (40%), Gaps = 10/89 (11%)
Frame = +2
Query: 174 WAVDVVYLKFFRN-----IDLVSSFSWGVAALAHLY*ELNNAARWNCS-----QMAGYLT 223
W + V+++ F N + +S F G+ L + L+ RWN + + Y+
Sbjct: 506 WCLLVIFMFFLPNEIISFYETISKFGSGMFLLVQVVLLLDFVHRWNDTWVGYDEQFWYIA 685
Query: 224 LFVYGLYIYTWLSLVQAWVFHHFPGMGSN 252
LFV L Y + +FH F G +
Sbjct: 686 LFVVSLVCYVATFVFSGVLFHFFTPSGQD 772
>TC76797 homologue to GP|8954298|gb|AAD27882.2| chlorophyll a/b-binding
protein CP24 precursor {Vigna radiata}, partial (93%)
Length = 1206
Score = 30.4 bits (67), Expect = 0.91
Identities = 34/139 (24%), Positives = 57/139 (40%), Gaps = 18/139 (12%)
Frame = +3
Query: 177 DVVYLKFFRNIDLVSSFSWGVAALAHLY*-ELNNAARW-NCSQMAGYLTLFVYGLYIYTW 234
D +LK++R +L+ W +AA+ ++ + + W + F +G + T
Sbjct: 393 DPAFLKWYREAELIHG-RWAMAAVLGIFVGQAWSGVPWFEAGADPNAIAPFSFGTLLGTQ 569
Query: 235 LSLV-----QAWVFHHFPGMGSNE---VWENYVEN--------QYPRAMLFLPLSGLGTV 278
L L+ + WV P S E W EN YP F PLS GT+
Sbjct: 570 LILMGWVESKRWVDFFNPDSQSIEWATPWSKTAENFANATGEQGYPGGKFFDPLSLAGTI 749
Query: 279 DHYINYLDALDLTRVVMAQ 297
++ + D L R+ +A+
Sbjct: 750 ENGVYIPDTEKLERLKLAE 806
>TC82474
Length = 992
Score = 30.0 bits (66), Expect = 1.2
Identities = 16/54 (29%), Positives = 28/54 (51%)
Frame = -2
Query: 258 YVENQYPRAMLFLPLSGLGTVDHYINYLDALDLTRVVMAQYGVHRQARQFEQVS 311
Y E++ P A F G + ++Y+ +LD +++ V QY H Q F+ +S
Sbjct: 883 YTEDR-PHATRFSLAMGHKSPNNYMTFLDRVEVDDVSFNQYDFHHQTCPFDDIS 725
>BF633515
Length = 249
Score = 30.0 bits (66), Expect = 1.2
Identities = 12/20 (60%), Positives = 15/20 (75%)
Frame = +2
Query: 65 LDDVTYLLHIPIDDMLLSHG 84
LDDV L H+PI+ +LSHG
Sbjct: 2 LDDVACLTHLPIEGRMLSHG 61
>TC88489 similar to GP|14334978|gb|AAK59666.1 unknown protein {Arabidopsis
thaliana}, partial (47%)
Length = 1187
Score = 28.9 bits (63), Expect = 2.7
Identities = 21/57 (36%), Positives = 30/57 (51%)
Frame = +3
Query: 129 ECLKEQRDLAAEYGVTEEVERLQDQTVRIYLLYLVGITIFTDKSQWAVDVVYLKFFR 185
E L E + ++E V EE E+ ++ V L L G+++ K VDVV LKF R
Sbjct: 354 ETLPEGGEESSEKVVKEEEEKRVEE-VEEKDLSLWGVSLLPSKGNEGVDVVLLKFLR 521
>TC86205 similar to PIR|A86289|A86289 probable ABC transporter [imported] -
Arabidopsis thaliana, partial (52%)
Length = 2721
Score = 27.7 bits (60), Expect = 5.9
Identities = 11/33 (33%), Positives = 18/33 (54%)
Frame = +1
Query: 45 EMWHEETSSFHLPFEEITVTLDDVTYLLHIPID 77
E H + LPFE ++T DD+ Y + +P +
Sbjct: 625 ESSHGKKKGMVLPFEPHSITFDDIVYSVDMPAE 723
>CA920886 similar to GP|11994399|db contains similarity to receptor protein
kinase~gene_id:MIL23.20 {Arabidopsis thaliana}, partial
(25%)
Length = 818
Score = 27.3 bits (59), Expect = 7.7
Identities = 10/24 (41%), Positives = 17/24 (70%)
Frame = +3
Query: 179 VYLKFFRNIDLVSSFSWGVAALAH 202
+Y+ F+RNI ++S F W V+ +H
Sbjct: 438 IYIYFWRNIAILSIFWWHVSKCSH 509
>BG583982
Length = 868
Score = 27.3 bits (59), Expect = 7.7
Identities = 9/19 (47%), Positives = 12/19 (62%)
Frame = +2
Query: 234 WLSLVQAWVFHHFPGMGSN 252
W LV W+FHH +GS+
Sbjct: 362 WKGLVLLWLFHHIKSIGSS 418
>TC85062 similar to EGAD|119543|127780 hypothetical protein F49C5.k
{Caenorhabditis elegans}, partial (5%)
Length = 781
Score = 27.3 bits (59), Expect = 7.7
Identities = 14/35 (40%), Positives = 20/35 (57%)
Frame = -3
Query: 59 EEITVTLDDVTYLLHIPIDDMLLSHGFISRDEAVE 93
E + + +DDVT +++I D L G I D AVE
Sbjct: 710 ETVPLKVDDVTQIVNIVADTASLKDGEIGDDVAVE 606
>TC86206 similar to GP|20522008|dbj|BAB92011. pleiotropic drug resistance
like protein {Nicotiana tabacum}, partial (11%)
Length = 757
Score = 27.3 bits (59), Expect = 7.7
Identities = 10/30 (33%), Positives = 17/30 (56%)
Frame = +3
Query: 48 HEETSSFHLPFEEITVTLDDVTYLLHIPID 77
H + LPFE ++T DD+ Y + +P +
Sbjct: 207 HGKKKGMVLPFEPHSITFDDIVYSVDMPAE 296
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.326 0.140 0.445
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 10,793,187
Number of Sequences: 36976
Number of extensions: 161387
Number of successful extensions: 1007
Number of sequences better than 10.0: 32
Number of HSP's better than 10.0 without gapping: 1003
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1005
length of query: 311
length of database: 9,014,727
effective HSP length: 96
effective length of query: 215
effective length of database: 5,465,031
effective search space: 1174981665
effective search space used: 1174981665
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)
S2: 58 (26.9 bits)
Medicago: description of AC144893.1