BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC144807.2 + phase: 0
(239 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC82908 similar to GP|16648738|gb|AAL25561.1 F25I18.1/F25I18.1 {... 426 e-120
BF635435 similar to GP|16648738|gb F25I18.1/F25I18.1 {Arabidopsi... 252 1e-67
TC79728 similar to GP|22136692|gb|AAM91665.1 unknown protein {Ar... 38 0.004
TC85344 similar to GP|22758323|gb|AAN05527.1 putative glutamine ... 37 0.009
TC79027 similar to GP|9758377|dbj|BAB08826.1 Dreg-2 like protein... 30 0.63
TC89539 similar to PIR|T06672|T06672 probable aminoacyl-t-RNA sy... 30 0.82
AW587336 similar to GP|12313676|dbj putative calcium-dependent p... 30 1.1
TC78553 similar to GP|21536767|gb|AAM61099.1 GS1-like protein {A... 29 1.4
BG457872 similar to PIR|T49166|T49 hypothetical protein T20N10.1... 28 3.1
BI266478 similar to GP|10177173|db 5-oxoprolinase {Arabidopsis t... 27 5.3
TC88129 similar to GP|9758230|dbj|BAB08729.1 emb|CAB61996.1~gene... 27 7.0
TC77838 similar to GP|10998146|dbj|BAB03117. gene_id:MEC18.18~un... 27 9.1
AL384020 similar to GP|9759388|dbj Rab geranylgeranyltransferase... 27 9.1
>TC82908 similar to GP|16648738|gb|AAL25561.1 F25I18.1/F25I18.1 {Arabidopsis
thaliana}, partial (81%)
Length = 643
Score = 426 bits (1096), Expect = e-120
Identities = 208/209 (99%), Positives = 208/209 (99%)
Frame = +1
Query: 23 PKTRLRGVVFDMDGTLTVPVIDFPSMYKAVLGDDEYLRVKASNPVGIDILKLIDHWSPLQ 82
PK RLRGVVFDMDGTLTVPVIDFPSMYKAVLGDDEYLRVKASNPVGIDILKLIDHWSPLQ
Sbjct: 16 PKRRLRGVVFDMDGTLTVPVIDFPSMYKAVLGDDEYLRVKASNPVGIDILKLIDHWSPLQ 195
Query: 83 QRQAYDTIAHFEKQALDHLQIMPGAADLCNVLDSKKIRRGLITRNMKSAVDLFHQRFGIT 142
QRQAYDTIAHFEKQALDHLQIMPGAADLCNVLDSKKIRRGLITRNMKSAVDLFHQRFGIT
Sbjct: 196 QRQAYDTIAHFEKQALDHLQIMPGAADLCNVLDSKKIRRGLITRNMKSAVDLFHQRFGIT 375
Query: 143 FSPALSREFRPYKPDPAPLLHICSLWEVQPNEVIMVGDSLKDDIACGGRAGAHTCLLDQT 202
FSPALSREFRPYKPDPAPLLHICSLWEVQPNEVIMVGDSLKDDIACGGRAGAHTCLLDQT
Sbjct: 376 FSPALSREFRPYKPDPAPLLHICSLWEVQPNEVIMVGDSLKDDIACGGRAGAHTCLLDQT 555
Query: 203 GRYDSPEYANVDFKPDFKVTSLAEVYSIL 231
GRYDSPEYANVDFKPDFKVTSLAEVYSIL
Sbjct: 556 GRYDSPEYANVDFKPDFKVTSLAEVYSIL 642
>BF635435 similar to GP|16648738|gb F25I18.1/F25I18.1 {Arabidopsis thaliana},
partial (46%)
Length = 620
Score = 252 bits (643), Expect = 1e-67
Identities = 124/134 (92%), Positives = 126/134 (93%)
Frame = +3
Query: 102 QIMPGAADLCNVLDSKKIRRGLITRNMKSAVDLFHQRFGITFSPALSREFRPYKPDPAPL 161
QIMPGAADLCNVLDSKKIRRGLITRNMKSAVDLFHQRFGITFSPALSREFRPYKPDPAPL
Sbjct: 3 QIMPGAADLCNVLDSKKIRRGLITRNMKSAVDLFHQRFGITFSPALSREFRPYKPDPAPL 182
Query: 162 LHICSLWEVQPNEVIMVGDSLKDDIACGGRAGAHTCLLDQTGRYDSPEYANVDFKPDFKV 221
LHICSLWEVQPNEVIMVGDSLKDDIACGGRAGAHTCLLDQTGRYDSPEYANVDFKPDFK
Sbjct: 183 LHICSLWEVQPNEVIMVGDSLKDDIACGGRAGAHTCLLDQTGRYDSPEYANVDFKPDFK- 359
Query: 222 TSLAEVYSILETNF 235
+L EV S +F
Sbjct: 360 -NLEEVTSTFGGSF 398
>TC79728 similar to GP|22136692|gb|AAM91665.1 unknown protein {Arabidopsis
thaliana}, partial (79%)
Length = 1121
Score = 37.7 bits (86), Expect = 0.004
Identities = 45/198 (22%), Positives = 78/198 (38%), Gaps = 27/198 (13%)
Frame = +2
Query: 28 RGVVFDMDGTLTVPVIDFPSMYKAV-------LGDDEYL---RVKASNPVGIDILKLIDH 77
+ ++ D GTL +P +Y+ + ++E L R S P G L+ ++
Sbjct: 218 KALLVDAVGTLVLPSQPMAQIYRTIGEKYGVNYSEEEILYRYRRAYSQPWGKSRLRYVND 397
Query: 78 WSPLQQRQAYDTIAHFEKQALDHLQ---IMPGAADLCNVLDSKKIRRGLITRNMKSAVDL 134
P Q ++ + Q + L + A LC+ +++++ + L +K AV
Sbjct: 398 GRPFWQYIVSNSTGCSDSQYFEELYNYYMTDKAWHLCDP-NTEEVFKALRNSGVKLAVV- 571
Query: 135 FHQRFGITFSP--------------ALSREFRPYKPDPAPLLHICSLWEVQPNEVIMVGD 180
F P A+S E KP+P L C L +V+P + + VGD
Sbjct: 572 --SNFDTRLRPLLRALNCDDWFDAVAVSAEVEAEKPNPTIFLKACELLDVKPEDAVHVGD 745
Query: 181 SLKDDIACGGRAGAHTCL 198
++DI AG L
Sbjct: 746 DRRNDIWGARDAGCDAWL 799
>TC85344 similar to GP|22758323|gb|AAN05527.1 putative glutamine synthetase
{Oryza sativa (japonica cultivar-group)}, partial (42%)
Length = 1111
Score = 36.6 bits (83), Expect = 0.009
Identities = 35/167 (20%), Positives = 69/167 (40%), Gaps = 11/167 (6%)
Frame = +3
Query: 27 LRGVVFDMDGTLTVPVIDFPSMYKAVLGD-------DEYLRVKASNPVGIDILKLIDHWS 79
++ V+ D+DGTL ++ K LG E L++ P+ + D+
Sbjct: 336 IKCVILDLDGTLLNTDGIVCNVLKVSLGKYGKEWDGRETLKIVGKTPLEAASAVVEDYGL 515
Query: 80 PLQQRQAYDTIAHFEKQALDHLQIMPGAADLCNVLDSKKIRRGLITRNMKSAVDL---FH 136
P + ++ +++ +PGA L L S + L + + + ++D FH
Sbjct: 516 PCSPIEFVSELSPQFSDQWCNIKALPGANRLIKHLKSNGVPMALASNSPRESIDAKISFH 695
Query: 137 QRFGITFSPALS-REFRPYKPDPAPLLHICSLWEVQPNEVIMVGDSL 182
+ +FS + E R KP P +++P+ +++ DSL
Sbjct: 696 DGWKDSFSVIIGGEEVRTGKPSPDIFFEAARRLKIEPSSCLVIEDSL 836
>TC79027 similar to GP|9758377|dbj|BAB08826.1 Dreg-2 like protein
{Arabidopsis thaliana}, partial (96%)
Length = 1299
Score = 30.4 bits (67), Expect = 0.63
Identities = 18/56 (32%), Positives = 27/56 (48%), Gaps = 1/56 (1%)
Frame = +3
Query: 155 KPDPAPL-LHICSLWEVQPNEVIMVGDSLKDDIACGGRAGAHTCLLDQTGRYDSPE 209
KPDP + + + P E + +GDS++ D G H LLD R+ +PE
Sbjct: 822 KPDPKIYEIALERAGNIAPEEALHIGDSMRKDYEPAKSIGMHALLLD---RFKTPE 980
>TC89539 similar to PIR|T06672|T06672 probable aminoacyl-t-RNA synthetase
(EC 6.1.1.-) - Arabidopsis thaliana, partial (26%)
Length = 1382
Score = 30.0 bits (66), Expect = 0.82
Identities = 22/72 (30%), Positives = 36/72 (49%)
Frame = +3
Query: 12 LFPRIMSSHLTPKTRLRGVVFDMDGTLTVPVIDFPSMYKAVLGDDEYLRVKASNPVGIDI 71
LFP+++ ++ P +RG ++DG V F + VL + +L VK S G+DI
Sbjct: 690 LFPKVVEAYSRPTRIVRGKEDELDGEYQVDEAAFEINEERVLW-NTFLSVKKSINPGLDI 866
Query: 72 LKLIDHWSPLQQ 83
++ S L Q
Sbjct: 867 DDFVEISSQLIQ 902
>AW587336 similar to GP|12313676|dbj putative calcium-dependent protein
kinase {Oryza sativa (japonica cultivar-group)}, partial
(9%)
Length = 355
Score = 29.6 bits (65), Expect = 1.1
Identities = 12/26 (46%), Positives = 17/26 (65%)
Frame = +3
Query: 138 RFGITFSPALSREFRPYKPDPAPLLH 163
++G T +PA +RE P P P P+LH
Sbjct: 45 QWGATAAPAPARESTPQPPPPIPILH 122
>TC78553 similar to GP|21536767|gb|AAM61099.1 GS1-like protein {Arabidopsis
thaliana}, partial (93%)
Length = 1110
Score = 29.3 bits (64), Expect = 1.4
Identities = 41/182 (22%), Positives = 71/182 (38%), Gaps = 18/182 (9%)
Frame = +1
Query: 18 SSHLTPKTRLRGVVFDMDGTLTVPVIDFPSMYKAVLGD-----DEYLRVKASNPVGIDIL 72
SS + + + VVFDMDG L + + + +L + L+ K I+
Sbjct: 97 SSSVGVRKPITHVVFDMDGLLLDTEKFYTEVQEIILARYNKTFEWPLKAKMMGMKAIEAA 276
Query: 73 KLIDHWSPLQQRQAYDTIAHFEKQALDHL----QIMPGAADLCNVLDSKKIRRGLITRNM 128
K+ + + + D + L L ++MPG + L L +K + + T +
Sbjct: 277 KVFVEETGISDSLSADQFLVEREDMLRSLFPTSELMPGVSRLLKHLHAKGVPIAVATGSH 456
Query: 129 KSAVDLFHQRFGITFS------PALSREFRPYKPDPAPLLHICSLWE---VQPNEVIMVG 179
K +L QR G FS E + KP P L +E V P+ +++
Sbjct: 457 KRHFELKTQRHGEMFSLMHHVVLGDDPEVKQGKPSPDVFLAAARRFEGGPVDPSNILVFE 636
Query: 180 DS 181
D+
Sbjct: 637 DA 642
>BG457872 similar to PIR|T49166|T49 hypothetical protein T20N10.180 -
Arabidopsis thaliana, partial (39%)
Length = 598
Score = 28.1 bits (61), Expect = 3.1
Identities = 17/51 (33%), Positives = 25/51 (48%), Gaps = 12/51 (23%)
Frame = +3
Query: 3 LLSSTKAFLLFPRIMSSHLT-PKTR-----------LRGVVFDMDGTLTVP 41
+L ST L P++ H++ P R +G+VFD D T+TVP
Sbjct: 225 ILCSTMVILKDPKLSLPHISVPDIRYIDWAALRRKGFKGIVFDKDNTITVP 377
>BI266478 similar to GP|10177173|db 5-oxoprolinase {Arabidopsis thaliana},
partial (7%)
Length = 297
Score = 27.3 bits (59), Expect = 5.3
Identities = 14/34 (41%), Positives = 18/34 (52%), Gaps = 3/34 (8%)
Frame = +2
Query: 186 IACGGRAGA-HTCLLDQTGRYDSPEYANV--DFK 216
+AC G AG H C Q RY+ Y+ + DFK
Sbjct: 89 LACFGGAGPQHACAYSQVSRYERSAYSQILWDFK 190
>TC88129 similar to GP|9758230|dbj|BAB08729.1
emb|CAB61996.1~gene_id:MZF18.6~strong similarity to
unknown protein {Arabidopsis thaliana}, partial (15%)
Length = 953
Score = 26.9 bits (58), Expect = 7.0
Identities = 7/17 (41%), Positives = 13/17 (76%)
Frame = -3
Query: 162 LHICSLWEVQPNEVIMV 178
+ IC +W++ PN +IM+
Sbjct: 627 MFICQIWQIWPNNIIMI 577
>TC77838 similar to GP|10998146|dbj|BAB03117. gene_id:MEC18.18~unknown
protein {Arabidopsis thaliana}, partial (86%)
Length = 1353
Score = 26.6 bits (57), Expect = 9.1
Identities = 10/23 (43%), Positives = 15/23 (64%), Gaps = 3/23 (13%)
Frame = -3
Query: 151 FRPYKP---DPAPLLHICSLWEV 170
FRP+K P P++ +CS+W V
Sbjct: 121 FRPFKTIGFGPVPVVDVCSVWSV 53
>AL384020 similar to GP|9759388|dbj Rab geranylgeranyltransferase beta
subunit {Arabidopsis thaliana}, partial (39%)
Length = 444
Score = 26.6 bits (57), Expect = 9.1
Identities = 19/53 (35%), Positives = 26/53 (48%), Gaps = 1/53 (1%)
Frame = +1
Query: 113 VLDSKKIRRGLITRNMKSAVDLFHQRFGITFSPALSREFRPYKP-DPAPLLHI 164
+LD + G I+ AVD+FH FG+ L E+ KP DPA L +
Sbjct: 187 ILDCQDTDHGGISDRPDDAVDVFHTYFGVAGLSLL--EYPGVKPIDPAYALPV 339
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.322 0.139 0.421
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 7,312,688
Number of Sequences: 36976
Number of extensions: 102975
Number of successful extensions: 443
Number of sequences better than 10.0: 26
Number of HSP's better than 10.0 without gapping: 443
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 443
length of query: 239
length of database: 9,014,727
effective HSP length: 93
effective length of query: 146
effective length of database: 5,575,959
effective search space: 814090014
effective search space used: 814090014
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 57 (26.6 bits)
Medicago: description of AC144807.2