BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC144806.1 + phase: 0
(260 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC82223 315 4e-89
AL375461 weakly similar to GP|7300610|gb|A CG17203 gene product ... 32 0.32
BQ141541 similar to GP|334068|gb|A ORF2 {Pseudorabies virus}, pa... 30 0.71
CA990541 weakly similar to GP|9758600|dbj gene_id:MDF20.10~pir||... 29 1.6
BQ139812 weakly similar to GP|4415927|gb|A unknown protein {Arab... 28 2.7
AW685995 similar to PIR|I51618|I516 nucleolar phosphoprotein - A... 28 3.5
CB894947 28 4.6
BQ144057 similar to GP|1483567|emb| viral proteinase {Pseudorabi... 28 4.6
BQ141682 similar to GP|6523547|emb| hydroxyproline-rich glycopro... 27 6.0
BQ141680 similar to GP|11993889|gb| virion-associated nuclear-sh... 27 6.0
TC81939 similar to GP|23617228|dbj|BAC20896. putative SUMO activ... 27 6.0
TC84387 homologue to GP|15217309|gb|AAK92653.1 Hypothetical prot... 27 7.9
BQ137244 similar to GP|20161222|dbj Epstein-Barr virus EBNA-1-li... 27 7.9
BI309706 homologue to GP|18175903|gb putative calcium-dependent ... 27 7.9
>TC82223
Length = 606
Score = 315 bits (807), Expect(2) = 4e-89
Identities = 162/165 (98%), Positives = 163/165 (98%)
Frame = +2
Query: 1 MDFHTLSRKELQALSKMNKIPANITNVAMADALSALPHVEGLDEILNQREGGDIGTPAVQ 60
MDFHTLSRKELQALSKMNKIPANITNVAMADALSALPHVEGLDEILNQREGGDIGTPAVQ
Sbjct: 59 MDFHTLSRKELQALSKMNKIPANITNVAMADALSALPHVEGLDEILNQREGGDIGTPAVQ 238
Query: 61 PRTARRTTTQRKPVKEAESTKVSTRVNRGGRGGVAEGEVEQENLDANVDAGTPAVVPTSR 120
PRTARRTTTQRKPVKEAESTKVSTRVNRGGRGGVAEGEVEQENLDANVDAGTPAVVPTSR
Sbjct: 239 PRTARRTTTQRKPVKEAESTKVSTRVNRGGRGGVAEGEVEQENLDANVDAGTPAVVPTSR 418
Query: 121 RRVPAVSTRRKKEVIVIEDEDDVVSEVQGKATDVAKTPAAAPSSR 165
RRVPAVSTRRKKEVIVIEDEDDVVSEVQGKATDVAKTPAAAP +
Sbjct: 419 RRVPAVSTRRKKEVIVIEDEDDVVSEVQGKATDVAKTPAAAPXXK 553
Score = 30.4 bits (67), Expect(2) = 4e-89
Identities = 14/21 (66%), Positives = 14/21 (66%)
Frame = +3
Query: 162 PSSRTRAGRSVRNKTEISDGT 182
P R G SV NKTEISDGT
Sbjct: 543 PXXRNXTGPSVPNKTEISDGT 605
>AL375461 weakly similar to GP|7300610|gb|A CG17203 gene product {Drosophila
melanogaster}, partial (15%)
Length = 524
Score = 31.6 bits (70), Expect = 0.32
Identities = 34/132 (25%), Positives = 53/132 (39%), Gaps = 5/132 (3%)
Frame = +2
Query: 47 NQREGGDIG----TPAVQPRTARRTTTQRKPVKEAESTKVSTRVN-RGGRGGVAEGEVEQ 101
N RE D G + VQP TTT R ++ A T + R + R GRG
Sbjct: 14 NLREQLDAGLLEVSTNVQPTPTTTTTTTRLLLQPATPTTTTPRTSSRRGRG--------T 169
Query: 102 ENLDANVDAGTPAVVPTSRRRVPAVSTRRKKEVIVIEDEDDVVSEVQGKATDVAKTPAAA 161
N A + P PT+ + R++K ++ +DE+ + Q +A + +P
Sbjct: 170 RNTPA-ASSPPPPPPPTTNGESVSRRGRKRKVTVIKDDEESEDEQQQQQAIASSSSPPPP 346
Query: 162 PSSRTRAGRSVR 173
P A V+
Sbjct: 347 PPPTAAAAPPVQ 382
>BQ141541 similar to GP|334068|gb|A ORF2 {Pseudorabies virus}, partial (2%)
Length = 1320
Score = 30.4 bits (67), Expect = 0.71
Identities = 16/41 (39%), Positives = 21/41 (51%)
Frame = -1
Query: 52 GDIGTPAVQPRTARRTTTQRKPVKEAESTKVSTRVNRGGRG 92
G GTP+ P + T + K+ E T+ TR RGGRG
Sbjct: 135 GHGGTPSPDPPLSSGETRRGSLRKKTEETRPHTREERGGRG 13
>CA990541 weakly similar to GP|9758600|dbj
gene_id:MDF20.10~pir||T08929~similar to unknown protein
{Arabidopsis thaliana}, partial (13%)
Length = 712
Score = 29.3 bits (64), Expect = 1.6
Identities = 34/154 (22%), Positives = 59/154 (38%), Gaps = 9/154 (5%)
Frame = +2
Query: 94 VAEGEVEQENLDANVDAGTPAVVPTSRRRVPAVSTRRKKEVIVIEDEDDVVSEVQGKATD 153
+AE E + T + PTSRR S + +K++ ED ++E + ATD
Sbjct: 257 LAEQEKSAKEKRIAKQGSTGSGTPTSRR-----SAKSRKKI-----EDSSIAEKKKTATD 406
Query: 154 VAKTPAAAPSSRTRAGRSVRNKTEISDGTSVQKAYSTRRSVRLVGKSLSKMSLADTEDME 213
S N+ ++SD + +K ++ + G S S + +
Sbjct: 407 TES------ESEKDEENKEENEIDVSDKSEDEKPIKGKKHIAKQGSSRSGTASSRRSAKS 568
Query: 214 STKNDDV---------SEEMSVSQNEGGSIETEN 238
KN+D+ ++ S S+ E E EN
Sbjct: 569 QKKNEDLFVAKKRKTTTDIESESEKEDHKEENEN 670
>BQ139812 weakly similar to GP|4415927|gb|A unknown protein {Arabidopsis
thaliana}, partial (50%)
Length = 307
Score = 28.5 bits (62), Expect = 2.7
Identities = 24/97 (24%), Positives = 40/97 (40%), Gaps = 2/97 (2%)
Frame = -2
Query: 141 DDVVSEVQGKATDVAKTPAAAPSSRTRAGRSVRNKTE--ISDGTSVQKAYSTRRSVRLVG 198
DD++SEV A+ K + + SSR R ++ KT + + + + S G
Sbjct: 291 DDIISEVAILASLAIKAESTSLSSRARTPTAIT*KTPAMFPNLRRPPRETAAQHSRTRTG 112
Query: 199 KSLSKMSLADTEDMESTKNDDVSEEMSVSQNEGGSIE 235
S ++ D ED+ + + S E G IE
Sbjct: 111 FSAARSLREDMEDLRTDEESSASNERRT*IASGAGIE 1
>AW685995 similar to PIR|I51618|I516 nucleolar phosphoprotein - African
clawed frog, partial (5%)
Length = 516
Score = 28.1 bits (61), Expect = 3.5
Identities = 21/101 (20%), Positives = 40/101 (38%)
Frame = +3
Query: 139 DEDDVVSEVQGKATDVAKTPAAAPSSRTRAGRSVRNKTEISDGTSVQKAYSTRRSVRLVG 198
+ED SE + D A AAP+ + A + ++ E S S + +
Sbjct: 177 EEDSSSSEESSSSEDEAPAKKAAPAKKAAAKKESSSEEESSSSES-----ESEEEEKPAK 341
Query: 199 KSLSKMSLADTEDMESTKNDDVSEEMSVSQNEGGSIETENG 239
K+ +K + E+ S++ + EE + ++ E G
Sbjct: 342 KAAAKKESSSEEESSSSEEESDEEEKNEARRR*EQEEARRG 464
>CB894947
Length = 658
Score = 27.7 bits (60), Expect = 4.6
Identities = 14/32 (43%), Positives = 19/32 (58%)
Frame = -1
Query: 172 VRNKTEISDGTSVQKAYSTRRSVRLVGKSLSK 203
+++ E+S GTS Q + RRS RL G S K
Sbjct: 118 IQSSAELSAGTSTQSFPTLRRSTRLGGNSGGK 23
>BQ144057 similar to GP|1483567|emb| viral proteinase {Pseudorabies virus},
partial (5%)
Length = 1295
Score = 27.7 bits (60), Expect = 4.6
Identities = 19/64 (29%), Positives = 26/64 (39%)
Frame = +3
Query: 37 PHVEGLDEILNQREGGDIGTPAVQPRTARRTTTQRKPVKEAESTKVSTRVNRGGRGGVAE 96
P+ + D GG + P PR T +R+P + + R RG G AE
Sbjct: 420 PNPKRQDAARGTPTGGGVPRPGPGPRPVAATDPRRQPQNKVNEGR--RREKRGRAGPRAE 593
Query: 97 GEVE 100
GE E
Sbjct: 594 GEGE 605
>BQ141682 similar to GP|6523547|emb| hydroxyproline-rich glycoprotein DZ-HRGP
{Volvox carteri f. nagariensis}, partial (8%)
Length = 938
Score = 27.3 bits (59), Expect = 6.0
Identities = 22/85 (25%), Positives = 35/85 (40%)
Frame = +3
Query: 49 REGGDIGTPAVQPRTARRTTTQRKPVKEAESTKVSTRVNRGGRGGVAEGEVEQENLDANV 108
REGG P +PR +P ++ +S+ GRGG A G
Sbjct: 51 REGGGARQPERKPR---------QPCQKPTPQTISSPPRH*GRGGAARGA-------GGG 182
Query: 109 DAGTPAVVPTSRRRVPAVSTRRKKE 133
+ G P + + ++R +T RKK+
Sbjct: 183 EGGGPPRMGSHKQRQNCGTTERKKK 257
>BQ141680 similar to GP|11993889|gb| virion-associated nuclear-shuttling
protein {Mus musculus}, partial (3%)
Length = 1156
Score = 27.3 bits (59), Expect = 6.0
Identities = 13/30 (43%), Positives = 16/30 (53%)
Frame = +3
Query: 70 QRKPVKEAESTKVSTRVNRGGRGGVAEGEV 99
Q +KE S V R+NR GRGG E+
Sbjct: 3 QNSGIKERRSRGVGGRMNRRGRGGRQTAEI 92
>TC81939 similar to GP|23617228|dbj|BAC20896. putative SUMO activating
enzyme 2 {Oryza sativa (japonica cultivar-group)},
partial (11%)
Length = 706
Score = 27.3 bits (59), Expect = 6.0
Identities = 24/81 (29%), Positives = 36/81 (43%), Gaps = 8/81 (9%)
Frame = +2
Query: 72 KPVKEAESTKVSTRVNRGGRGGVAEGEVEQENLDANVDAGTPAVVPTSRRRVP------- 124
+PV AE+ S N EVE+ D +V + P +R++P
Sbjct: 206 QPVAAAENEDKSVS-NGANTSDAPITEVEKSENDDDVGI----ISPVKKRKLPDDSDKSN 370
Query: 125 -AVSTRRKKEVIVIEDEDDVV 144
A R K++ VI+DEDD+V
Sbjct: 371 TADDERHHKKLQVIDDEDDLV 433
>TC84387 homologue to GP|15217309|gb|AAK92653.1 Hypothetical protein {Oryza
sativa} [Oryza sativa (japonica cultivar-group)],
partial (3%)
Length = 1130
Score = 26.9 bits (58), Expect = 7.9
Identities = 14/47 (29%), Positives = 27/47 (56%), Gaps = 2/47 (4%)
Frame = -3
Query: 13 ALSKMNKIPANITNVA--MADALSALPHVEGLDEILNQREGGDIGTP 57
AL+K+ + A++ +VA + +A + H D + + + GD+GTP
Sbjct: 639 ALAKVCPLSASLAHVASDLFEAKLVVDHAAESDAVAEELKTGDLGTP 499
>BQ137244 similar to GP|20161222|dbj Epstein-Barr virus EBNA-1-like protein
{Oryza sativa (japonica cultivar-group)}, partial (5%)
Length = 1050
Score = 26.9 bits (58), Expect = 7.9
Identities = 13/36 (36%), Positives = 19/36 (52%)
Frame = +1
Query: 158 PAAAPSSRTRAGRSVRNKTEISDGTSVQKAYSTRRS 193
PA R RAG S R +T + G ++ +TRR+
Sbjct: 340 PARRTEERERAGASARPQTRSAQGREHERHINTRRA 447
>BI309706 homologue to GP|18175903|gb putative calcium-dependent protein
kinase {Arabidopsis thaliana}, partial (36%)
Length = 758
Score = 26.9 bits (58), Expect = 7.9
Identities = 31/123 (25%), Positives = 47/123 (38%)
Frame = +1
Query: 96 EGEVEQENLDANVDAGTPAVVPTSRRRVPAVSTRRKKEVIVIEDEDDVVSEVQGKATDVA 155
+GE Q+N DAN D+ P S+ P S K I V G+ +
Sbjct: 55 KGENNQQNNDANGDSSATTPPPWSKPS-PQPSKPSKPSAI---------GPVLGRPMEDV 204
Query: 156 KTPAAAPSSRTRAGRSVRNKTEISDGTSVQKAYSTRRSVRLVGKSLSKMSLADTEDMEST 215
K A S GR T + + K Y+ K+++K LA+ ED+E
Sbjct: 205 K---ATYSMGKELGRGQFGVTHLCTHKTTGKQYAC--------KTIAKRKLANKEDIEDV 351
Query: 216 KND 218
+ +
Sbjct: 352 RRE 360
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.310 0.126 0.332
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 5,099,608
Number of Sequences: 36976
Number of extensions: 45763
Number of successful extensions: 346
Number of sequences better than 10.0: 28
Number of HSP's better than 10.0 without gapping: 341
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 345
length of query: 260
length of database: 9,014,727
effective HSP length: 94
effective length of query: 166
effective length of database: 5,538,983
effective search space: 919471178
effective search space used: 919471178
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.8 bits)
S2: 57 (26.6 bits)
Medicago: description of AC144806.1