BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC144765.21 - phase: 0
(471 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC80132 similar to PIR|D96538|D96538 cytosolic tRNA-Ala syntheta... 550 e-157
TC77588 similar to PIR|D96538|D96538 cytosolic tRNA-Ala syntheta... 508 e-144
TC77587 weakly similar to PIR|D96538|D96538 cytosolic tRNA-Ala s... 155 3e-38
TC93821 similar to GP|10177227|dbj|BAB10601. alanyl-tRNA synthet... 86 2e-17
BF641925 similar to GP|10177227|db alanyl-tRNA synthetase {Arabi... 51 1e-06
BG453428 similar to PIR|S48032|S48 cim1 protein - soybean, parti... 33 0.24
TC85063 similar to PIR|T47959|T47959 hypothetical protein F15G16... 32 0.53
TC86542 weakly similar to GP|19698991|gb|AAL91231.1 EF-hand calc... 29 3.4
AL383717 29 3.4
TC89649 similar to PIR|T14276|T14276 myosin-like protein my2 - c... 28 7.6
TC83019 similar to GP|4049632|gb|AAC97601.1| pyruvate dehydrogen... 28 7.6
TC86371 similar to GP|21592923|gb|AAM64873.1 induced upon woundi... 28 9.9
>TC80132 similar to PIR|D96538|D96538 cytosolic tRNA-Ala synthetase
[imported] - Arabidopsis thaliana, partial (25%)
Length = 908
Score = 550 bits (1416), Expect = e-157
Identities = 257/257 (100%), Positives = 257/257 (100%)
Frame = +1
Query: 1 MPETESIDIEWPAKRVRDTFAKFFEEKNHVHWKSSAVVPLNDPTLLFANAGMNQFKPIFL 60
MPETESIDIEWPAKRVRDTFAKFFEEKNHVHWKSSAVVPLNDPTLLFANAGMNQFKPIFL
Sbjct: 136 MPETESIDIEWPAKRVRDTFAKFFEEKNHVHWKSSAVVPLNDPTLLFANAGMNQFKPIFL 315
Query: 61 GTVDPNTALSKLTRACNIQKCIRAGGKHNDLDDVGKDTYHHTFFEMLGNWSFGDYFKEEA 120
GTVDPNTALSKLTRACNIQKCIRAGGKHNDLDDVGKDTYHHTFFEMLGNWSFGDYFKEEA
Sbjct: 316 GTVDPNTALSKLTRACNIQKCIRAGGKHNDLDDVGKDTYHHTFFEMLGNWSFGDYFKEEA 495
Query: 121 ISWAWELLTEVYNLPSDRIYVTYFGGDEKTGLGPDNEARDIWLKFLPPGRVLPFGSKDNF 180
ISWAWELLTEVYNLPSDRIYVTYFGGDEKTGLGPDNEARDIWLKFLPPGRVLPFGSKDNF
Sbjct: 496 ISWAWELLTEVYNLPSDRIYVTYFGGDEKTGLGPDNEARDIWLKFLPPGRVLPFGSKDNF 675
Query: 181 WEMGDTGPCGPCSEIHFDRIGNRNASSFVNDEDPNCIELWNLVFIQFNREADGSLKSLPA 240
WEMGDTGPCGPCSEIHFDRIGNRNASSFVNDEDPNCIELWNLVFIQFNREADGSLKSLPA
Sbjct: 676 WEMGDTGPCGPCSEIHFDRIGNRNASSFVNDEDPNCIELWNLVFIQFNREADGSLKSLPA 855
Query: 241 KHVDTGMGFERLTSILQ 257
KHVDTGMGFERLTSILQ
Sbjct: 856 KHVDTGMGFERLTSILQ 906
>TC77588 similar to PIR|D96538|D96538 cytosolic tRNA-Ala synthetase
[imported] - Arabidopsis thaliana, partial (64%)
Length = 2100
Score = 508 bits (1307), Expect = e-144
Identities = 250/296 (84%), Positives = 265/296 (89%)
Frame = +2
Query: 167 PPGRVLPFGSKDNFWEMGDTGPCGPCSEIHFDRIGNRNASSFVNDEDPNCIELWNLVFIQ 226
PPGRVLPFG KDNFWEMGDTGPCGPC+EIHFDRIGNR+A+S VN++DP CIE+WNLVFIQ
Sbjct: 2 PPGRVLPFGCKDNFWEMGDTGPCGPCTEIHFDRIGNRDAASLVNNDDPTCIEIWNLVFIQ 181
Query: 227 FNREADGSLKSLPAKHVDTGMGFERLTSILQHKNSNYDTDVFVPIFDAIQQATGARPYSG 286
FNREAD SLKSLPAKHVDTGMGFERLTSILQ K SNYDTDVF+PIFDAIQ ATGARPYSG
Sbjct: 182 FNREADSSLKSLPAKHVDTGMGFERLTSILQRKLSNYDTDVFMPIFDAIQLATGARPYSG 361
Query: 287 KVGPHDADKVDMAYRVVADHIRTLSIAIADGSQPGNVGREYVLRRILRRAVRYGREVLKA 346
KVGP DADK+DMAYRVVADHIRTLS AIADGS+PGN GREYVLRRILRRAVRYGREVLKA
Sbjct: 362 KVGPEDADKIDMAYRVVADHIRTLSFAIADGSRPGNEGREYVLRRILRRAVRYGREVLKA 541
Query: 347 EEGFFNGLVNVVANVLGDVFPELKQNEERIRKMIQVEEESFSRNLFKGIRKFNKAVEHVQ 406
EEGFFNGLVNVVANVLGDVFPELKQ E IR +IQ EEESF R L KGI KF AV HVQ
Sbjct: 542 EEGFFNGLVNVVANVLGDVFPELKQQEVHIRNVIQEEEESFGRTLVKGIAKFKTAVGHVQ 721
Query: 407 GNILSGEATFELSHTFGFPLDLTQLMAEEKKFSVDIEGFHRAMKAARERSKTAPKK 462
GNILSGE FEL T+GFPLDLTQLMAEEKK VD+EGF+ AM+AARERS+TA K
Sbjct: 722 GNILSGEVAFELWDTYGFPLDLTQLMAEEKKLKVDVEGFNSAMEAARERSRTAQNK 889
>TC77587 weakly similar to PIR|D96538|D96538 cytosolic tRNA-Ala synthetase
[imported] - Arabidopsis thaliana, partial (21%)
Length = 1024
Score = 155 bits (392), Expect = 3e-38
Identities = 77/79 (97%), Positives = 77/79 (97%)
Frame = +1
Query: 384 EESFSRNLFKGIRKFNKAVEHVQGNILSGEATFELSHTFGFPLDLTQLMAEEKKFSVDIE 443
EESFSRNLFKGIRKFNKAVEHVQGNILSGE FELSHTFGFPLDLTQLMAEEKKFSVDIE
Sbjct: 1 EESFSRNLFKGIRKFNKAVEHVQGNILSGEVAFELSHTFGFPLDLTQLMAEEKKFSVDIE 180
Query: 444 GFHRAMKAARERSKTAPKK 462
GFHRAMKAARERSKTAPKK
Sbjct: 181 GFHRAMKAARERSKTAPKK 237
>TC93821 similar to GP|10177227|dbj|BAB10601. alanyl-tRNA synthetase
{Arabidopsis thaliana}, partial (10%)
Length = 641
Score = 86.3 bits (212), Expect = 2e-17
Identities = 48/113 (42%), Positives = 70/113 (61%)
Frame = +3
Query: 16 VRDTFAKFFEEKNHVHWKSSAVVPLNDPTLLFANAGMNQFKPIFLGTVDPNTALSKLTRA 75
+R+ F F+ + H S+++VP +DPT+L AGM QFKPIFLG + ++ RA
Sbjct: 330 IRERFLSFYASRGHKVLPSASLVP-DDPTVLLTIAGMLQFKPIFLGKLP-----RQVPRA 491
Query: 76 CNIQKCIRAGGKHNDLDDVGKDTYHHTFFEMLGNWSFGDYFKEEAISWAWELL 128
Q+CIR ND+++VG + HHTFFEMLGN+SFG F++ + S L+
Sbjct: 492 TTSQRCIRT----NDINNVGHTSRHHTFFEMLGNFSFG*LFQKTSYSMGMGLI 638
>BF641925 similar to GP|10177227|db alanyl-tRNA synthetase {Arabidopsis
thaliana}, partial (13%)
Length = 618
Score = 50.8 bits (120), Expect = 1e-06
Identities = 33/108 (30%), Positives = 55/108 (50%), Gaps = 2/108 (1%)
Frame = +3
Query: 358 VANVLGDVFPELKQNEERIRKMIQVEEESFSRNLFKGIRKFNKAVEHVQGNI--LSGEAT 415
V N + ELK+ E R + ++ E+ L I ++ G + L+GE
Sbjct: 102 VKNRAPRILEELKREELRFVQTLERGEKLLEEKLVDAITSAERS-----GTVPCLAGEDV 266
Query: 416 FELSHTFGFPLDLTQLMAEEKKFSVDIEGFHRAMKAARERSKTAPKKV 463
F L T+GFP+++T+ +AEE+ S+D+ GF M+ R +S+ A V
Sbjct: 267 FILYDTYGFPMEITKEVAEERGVSIDMNGFDIEMEKQRRQSQAAHNTV 410
>BG453428 similar to PIR|S48032|S48 cim1 protein - soybean, partial (53%)
Length = 638
Score = 33.1 bits (74), Expect = 0.24
Identities = 27/100 (27%), Positives = 45/100 (45%), Gaps = 7/100 (7%)
Frame = +3
Query: 189 CGPCSEIHFDRIGNRNASSFVNDEDPNCIELWNLVFIQFNREADGSLKSLPAKHVDTGMG 248
CG +E HFD G+ S V+ ED +V I+ R S A HVD+G
Sbjct: 6 CGSDAEYHFDLSGSAFGSMAVSGEDEELRNAGKIV-IEHRRVQCNYGGSTIAFHVDSGSN 182
Query: 249 FERLTSILQHKNSNYDTD-------VFVPIFDAIQQATGA 281
E ++++++ + D + + +D +QQ+ GA
Sbjct: 183 QEYFAALIEYEEGDGDLNKVELKEALDSSSWDTMQQSWGA 302
>TC85063 similar to PIR|T47959|T47959 hypothetical protein F15G16.60 -
Arabidopsis thaliana, partial (5%)
Length = 742
Score = 32.0 bits (71), Expect = 0.53
Identities = 15/35 (42%), Positives = 22/35 (62%)
Frame = -3
Query: 192 CSEIHFDRIGNRNASSFVNDEDPNCIELWNLVFIQ 226
C ++ F RIGNRN SS + + NC W+L+ +Q
Sbjct: 416 CMQLLFTRIGNRNTSSGIMNMLANC--SWDLITVQ 318
>TC86542 weakly similar to GP|19698991|gb|AAL91231.1 EF-hand calcium binding
protein-like {Arabidopsis thaliana}, partial (23%)
Length = 1085
Score = 29.3 bits (64), Expect = 3.4
Identities = 13/49 (26%), Positives = 25/49 (50%)
Frame = +2
Query: 215 NCIELWNLVFIQFNREADGSLKSLPAKHVDTGMGFERLTSILQHKNSNY 263
NC+ W +F +++++ G + L + G+G+ S+LQ S Y
Sbjct: 515 NCLGHWRGIFERYDKDRSGKIDPLELRDALYGIGYAVPASVLQLLLSKY 661
>AL383717
Length = 374
Score = 29.3 bits (64), Expect = 3.4
Identities = 18/47 (38%), Positives = 26/47 (55%), Gaps = 4/47 (8%)
Frame = -1
Query: 254 SILQHKNSNYDTDVFV---PIFDAIQQATG-ARPYSGKVGPHDADKV 296
SIL HKN Y + +F +F ++ A +RPY G PH A+K+
Sbjct: 353 SILCHKNCVYLSFLFSILQSVFSLLRAAVSISRPYPGITAPHKANKL 213
>TC89649 similar to PIR|T14276|T14276 myosin-like protein my2 - common
sunflower (fragment), partial (33%)
Length = 1356
Score = 28.1 bits (61), Expect = 7.6
Identities = 26/113 (23%), Positives = 45/113 (39%), Gaps = 1/113 (0%)
Frame = +3
Query: 287 KVGPHDADKVDMAYRVVADHIRTLSIAIADGSQPGNVGREYVLRRILR-RAVRYGREVLK 345
++ PH D+AYR + + ++ SI ++ S G +L R L R G E
Sbjct: 477 ELSPHVFAVADVAYRAMINEGKSNSILVSGESGAGKTETTKMLMRYLAYLGGRSGVEGRT 656
Query: 346 AEEGFFNGLVNVVANVLGDVFPELKQNEERIRKMIQVEEESFSRNLFKGIRKF 398
E+ N V G+ N R K ++++ ++ R IR +
Sbjct: 657 VEQQVLES--NPVLEAFGNAKTVRNNNSSRFGKFVEIQFDNKGRISGAAIRTY 809
>TC83019 similar to GP|4049632|gb|AAC97601.1| pyruvate dehydrogenase kinase
{Arabidopsis thaliana}, partial (89%)
Length = 1140
Score = 28.1 bits (61), Expect = 7.6
Identities = 13/32 (40%), Positives = 18/32 (55%)
Frame = +1
Query: 132 YNLPSDRIYVTYFGGDEKTGLGPDNEARDIWL 163
Y LP R+Y YFGGD + + +N D +L
Sbjct: 910 YGLPISRLYARYFGGDLQI-ISMENYGTDAYL 1002
>TC86371 similar to GP|21592923|gb|AAM64873.1 induced upon wounding stress
{Arabidopsis thaliana}, partial (98%)
Length = 1483
Score = 27.7 bits (60), Expect = 9.9
Identities = 21/80 (26%), Positives = 34/80 (42%), Gaps = 16/80 (20%)
Frame = +3
Query: 61 GTVDPNTALSKLTRACNIQKC-IRAGGKH--NDLDDVGKDTYH-------------HTFF 104
G + N + + A N++ I+ GGKH + GK YH H F+
Sbjct: 432 GAMLKNVLTAVIPNAPNLRHVSIQTGGKHYVGPFESFGKIKYHEPPYTEDMPRLDYHNFY 611
Query: 105 EMLGNWSFGDYFKEEAISWA 124
L + F + K+E +SW+
Sbjct: 612 YTLEDVLFEETGKKEGVSWS 671
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.320 0.139 0.425
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 14,808,419
Number of Sequences: 36976
Number of extensions: 210713
Number of successful extensions: 866
Number of sequences better than 10.0: 24
Number of HSP's better than 10.0 without gapping: 863
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 865
length of query: 471
length of database: 9,014,727
effective HSP length: 100
effective length of query: 371
effective length of database: 5,317,127
effective search space: 1972654117
effective search space used: 1972654117
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 60 (27.7 bits)
Medicago: description of AC144765.21