BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC144765.19 - phase: 0
(146 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC92582 similar to GP|11994195|dbj|BAB01298. contains similarity... 201 9e-53
BF638126 similar to GP|18087875|g hypothetical protein {Oryza sa... 88 1e-18
BQ148876 similar to GP|11994195|dbj contains similarity to trans... 35 0.011
AL383868 32 0.070
TC85290 similar to GP|7767653|gb|AAF69150.1| F27F5.2 {Arabidopsi... 32 0.091
AW689037 similar to GP|10177316|d kinesin-like protein {Arabidop... 31 0.16
TC83887 weakly similar to GP|13310891|gb|AAK13153.2 hypothetical... 30 0.27
TC90928 homologue to GP|21593435|gb|AAM65402.1 putative plant ad... 29 0.59
BG452832 weakly similar to GP|20976571|gb HYPOTHETICAL 90.9 KDA ... 28 1.0
TC79492 similar to GP|17064970|gb|AAL32639.1 Unknown protein {Ar... 28 1.3
BQ750979 similar to GP|13883467|gb PE_PGRS family protein {Mycob... 28 1.7
BI263137 weakly similar to PIR|T49263|T492 hypothetical protein ... 27 2.2
TC77585 similar to GP|17473863|gb|AAL38353.1 putative heat-shock... 27 2.2
TC92462 similar to PIR|F85436|F85436 hypothetical protein AT4g36... 27 2.9
TC80058 similar to GP|15451066|gb|AAK96804.1 Unknown protein {Ar... 27 2.9
BG580583 weakly similar to GP|9757901|dbj serine protease-like p... 27 2.9
AW127077 similar to PIR|T46060|T460 hypothetical protein T18N14.... 27 3.8
TC85996 weakly similar to GP|16648871|gb|AAL24287.1 Unknown prot... 27 3.8
BE248698 weakly similar to GP|12836661|db H6 homeo box 3~data so... 27 3.8
>TC92582 similar to GP|11994195|dbj|BAB01298. contains similarity to
transcription factor CA150b~gene_id:MPN9.8 {Arabidopsis
thaliana}, partial (20%)
Length = 1165
Score = 201 bits (511), Expect = 9e-53
Identities = 91/104 (87%), Positives = 96/104 (91%)
Frame = +2
Query: 43 VKNRAEEERKEKRAAQKAAIEGFKQLLDEASEDIDDKTDSHTFRKKWGNDPRFEALDRKE 102
+K ++ ++ AQKAAIEGFKQLLDEASEDIDDKTDSHTFRKKWGNDPRFEALDRKE
Sbjct: 545 LKIARKKNARKNVPAQKAAIEGFKQLLDEASEDIDDKTDSHTFRKKWGNDPRFEALDRKE 724
Query: 103 REHLLNERCHFEFNWTFLNSLNFIFFVCDSHNHHPYHWITYLDF 146
REHLLNERCHFEFNWTFLNSLNFIFFVCDSHNHHPYHWITYLDF
Sbjct: 725 REHLLNERCHFEFNWTFLNSLNFIFFVCDSHNHHPYHWITYLDF 856
Score = 118 bits (295), Expect = 1e-27
Identities = 57/59 (96%), Positives = 57/59 (96%)
Frame = +1
Query: 1 MLKERGVAPFSKWEKELPKIVFDPRFKAIPSYSARRSLFEHYVKNRAEEERKEKRAAQK 59
MLKERGVAPFSKWEKELPKIVFDPRFKAIPSYSARRSLFEHYVKNRAEEERKEKRA K
Sbjct: 418 MLKERGVAPFSKWEKELPKIVFDPRFKAIPSYSARRSLFEHYVKNRAEEERKEKRACTK 594
>BF638126 similar to GP|18087875|g hypothetical protein {Oryza sativa},
partial (14%)
Length = 637
Score = 88.2 bits (217), Expect = 1e-18
Identities = 40/40 (100%), Positives = 40/40 (100%)
Frame = +3
Query: 71 EASEDIDDKTDSHTFRKKWGNDPRFEALDRKEREHLLNER 110
EASEDIDDKTDSHTFRKKWGNDPRFEALDRKEREHLLNER
Sbjct: 9 EASEDIDDKTDSHTFRKKWGNDPRFEALDRKEREHLLNER 128
Score = 35.8 bits (81), Expect = 0.006
Identities = 25/75 (33%), Positives = 38/75 (50%), Gaps = 7/75 (9%)
Frame = +3
Query: 1 MLKERGVAPF-SKWEKELPKIVFDPRFKAIPSYSARRSLFEHY------VKNRAEEERKE 53
MLKE+G F S+W + + DPR+K++ + R LF Y V++ AE E +
Sbjct: 204 MLKEQGEITFNSRWSRVKESLRDDPRYKSV-KHEDRELLFNEYISELKAVEHAAERETRA 380
Query: 54 KRAAQKAAIEGFKQL 68
KR Q E ++L
Sbjct: 381 KREEQDKLREREREL 425
>BQ148876 similar to GP|11994195|dbj contains similarity to transcription
factor CA150b~gene_id:MPN9.8 {Arabidopsis thaliana},
partial (1%)
Length = 407
Score = 35.0 bits (79), Expect = 0.011
Identities = 23/65 (35%), Positives = 34/65 (51%), Gaps = 7/65 (10%)
Frame = +1
Query: 1 MLKERGVAPF-SKWEKELPKIVFDPRFKAIPSYSARRSLFEHY------VKNRAEEERKE 53
MLKE+G F S+W + + DPR+K++ + R LF Y V++ AE E +
Sbjct: 181 MLKEQGEITFNSRWSRVKESLRDDPRYKSV-KHEDRELLFNEYISELKAVEHAAERETRA 357
Query: 54 KRAAQ 58
KR Q
Sbjct: 358 KREEQ 372
>AL383868
Length = 314
Score = 32.3 bits (72), Expect = 0.070
Identities = 19/39 (48%), Positives = 24/39 (60%)
Frame = +1
Query: 95 FEALDRKEREHLLNERCHFEFNWTFLNSLNFIFFVCDSH 133
F L RKER+ NER + F T+L +NF FF+ DSH
Sbjct: 124 FLFLYRKERK---NERSKYFFFITYLLFINFSFFLLDSH 231
>TC85290 similar to GP|7767653|gb|AAF69150.1| F27F5.2 {Arabidopsis
thaliana}, partial (32%)
Length = 1558
Score = 32.0 bits (71), Expect = 0.091
Identities = 16/66 (24%), Positives = 32/66 (48%)
Frame = +3
Query: 44 KNRAEEERKEKRAAQKAAIEGFKQLLDEASEDIDDKTDSHTFRKKWGNDPRFEALDRKER 103
+ R E+E+KEK ++ + DE+ D D TD H +R++ + E R+
Sbjct: 795 EKRKEKEKKEKEREREKEKSKERHKKDESDSDNQDMTDGHGYREEKKKEKDKERKHRRRH 974
Query: 104 EHLLNE 109
+ +++
Sbjct: 975 QSSMDD 992
>AW689037 similar to GP|10177316|d kinesin-like protein {Arabidopsis
thaliana}, partial (13%)
Length = 662
Score = 31.2 bits (69), Expect = 0.16
Identities = 15/53 (28%), Positives = 26/53 (48%), Gaps = 4/53 (7%)
Frame = +2
Query: 88 KWGNDP----RFEALDRKEREHLLNERCHFEFNWTFLNSLNFIFFVCDSHNHH 136
KWGN+P R + D++ L R H +W + + + F C+ H+H+
Sbjct: 188 KWGNNPGWCDRA*SQDKRRNVVLSLSRFHVTCHWEYKHEQSIKSFTCNIHHHN 346
>TC83887 weakly similar to GP|13310891|gb|AAK13153.2 hypothetical protein
{Oryza sativa (japonica cultivar-group)}, partial (8%)
Length = 778
Score = 30.4 bits (67), Expect = 0.27
Identities = 16/85 (18%), Positives = 35/85 (40%)
Frame = +1
Query: 5 RGVAPFSKWEKELPKIVFDPRFKAIPSYSARRSLFEHYVKNRAEEERKEKRAAQKAAIEG 64
+ + SKWE + F+++ S + +FE YV EE ++ +R ++ +
Sbjct: 124 KDITESSKWEDFKQLLEDSQEFRSVGDVSLSKQMFEVYVAQLKEEAKENERKRKEERAKK 303
Query: 65 FKQLLDEASEDIDDKTDSHTFRKKW 89
K + + + R++W
Sbjct: 304 EKDREERERRKSKQRREKEGGRERW 378
>TC90928 homologue to GP|21593435|gb|AAM65402.1 putative plant adhesion
molecule {Arabidopsis thaliana}, partial (66%)
Length = 1058
Score = 29.3 bits (64), Expect = 0.59
Identities = 11/24 (45%), Positives = 16/24 (65%)
Frame = +3
Query: 122 SLNFIFFVCDSHNHHPYHWITYLD 145
+LN FF+CD H Y +I+Y+D
Sbjct: 870 NLNLRFFICDHMPHSLYSYISYVD 941
>BG452832 weakly similar to GP|20976571|gb HYPOTHETICAL 90.9 KDA PROTEIN
{Dictyostelium discoideum}, partial (2%)
Length = 669
Score = 28.5 bits (62), Expect = 1.0
Identities = 15/41 (36%), Positives = 23/41 (55%), Gaps = 5/41 (12%)
Frame = -3
Query: 105 HLLNE--RCHFEFNWTFLNSLNFIFFV---CDSHNHHPYHW 140
HL N +C F+FN+T+ N L+ + +SH+H HW
Sbjct: 130 HLDNSPYKCFFKFNYTYTNHLSRNSYKP**YNSHSHKMRHW 8
>TC79492 similar to GP|17064970|gb|AAL32639.1 Unknown protein {Arabidopsis
thaliana}, partial (21%)
Length = 1734
Score = 28.1 bits (61), Expect = 1.3
Identities = 10/26 (38%), Positives = 15/26 (57%)
Frame = +3
Query: 116 NWTFLNSLNFIFFVCDSHNHHPYHWI 141
N TF+ SL + F C NH +H++
Sbjct: 1551 NMTFIYSLRLLCFTCCGWNHRQFHFL 1628
>BQ750979 similar to GP|13883467|gb PE_PGRS family protein {Mycobacterium
tuberculosis CDC1551}, partial (1%)
Length = 728
Score = 27.7 bits (60), Expect = 1.7
Identities = 14/37 (37%), Positives = 21/37 (55%)
Frame = +1
Query: 48 EEERKEKRAAQKAAIEGFKQLLDEASEDIDDKTDSHT 84
E + K + QKAA G ++ +++D DDKTD T
Sbjct: 589 EHDVKPQPKKQKAATTGAQRQAAASADDADDKTDVKT 699
>BI263137 weakly similar to PIR|T49263|T492 hypothetical protein F12M12.190 -
Arabidopsis thaliana, partial (9%)
Length = 692
Score = 27.3 bits (59), Expect = 2.2
Identities = 14/44 (31%), Positives = 21/44 (46%), Gaps = 7/44 (15%)
Frame = -2
Query: 104 EHLLNERCHFE------FNWTF-LNSLNFIFFVCDSHNHHPYHW 140
+++L + HF+ F W +SL C +H H PYHW
Sbjct: 472 KYVLGYQDHFQMDLLMYFQWLSPFSSLALYQHACLNHYHSPYHW 341
>TC77585 similar to GP|17473863|gb|AAL38353.1 putative heat-shock protein
{Arabidopsis thaliana}, partial (92%)
Length = 3027
Score = 27.3 bits (59), Expect = 2.2
Identities = 12/27 (44%), Positives = 18/27 (66%)
Frame = +3
Query: 91 NDPRFEALDRKEREHLLNERCHFEFNW 117
NDP+F+ +D E++ +LNE C NW
Sbjct: 2400 NDPKFDHIDITEKQKVLNE-CVEAENW 2477
>TC92462 similar to PIR|F85436|F85436 hypothetical protein AT4g36970
[imported] - Arabidopsis thaliana, partial (23%)
Length = 1139
Score = 26.9 bits (58), Expect = 2.9
Identities = 18/55 (32%), Positives = 23/55 (41%), Gaps = 8/55 (14%)
Frame = -2
Query: 93 PRFEALDRKEREHLLNERCHFEFNWTFLNSLNF--------IFFVCDSHNHHPYH 139
P E L+ K H+ N+ WT +N L+ I F HNH PYH
Sbjct: 706 PHLERLEMKG--HIANQ-----VQWTLMNPLHHSG*AQN*NIAFHDQCHNHKPYH 563
>TC80058 similar to GP|15451066|gb|AAK96804.1 Unknown protein {Arabidopsis
thaliana}, partial (11%)
Length = 499
Score = 26.9 bits (58), Expect = 2.9
Identities = 10/23 (43%), Positives = 14/23 (60%)
Frame = -1
Query: 121 NSLNFIFFVCDSHNHHPYHWITY 143
N L+F D H+HH +H IT+
Sbjct: 73 NDLHFYLDHSDHHHHHHFHHITF 5
>BG580583 weakly similar to GP|9757901|dbj serine protease-like protein
{Arabidopsis thaliana}, partial (13%)
Length = 592
Score = 26.9 bits (58), Expect = 2.9
Identities = 11/29 (37%), Positives = 16/29 (54%)
Frame = -2
Query: 105 HLLNERCHFEFNWTFLNSLNFIFFVCDSH 133
HLL RC ++ F+ S + + VC SH
Sbjct: 327 HLLWYRCQYQIWLRFVRSFHLLLIVCVSH 241
>AW127077 similar to PIR|T46060|T460 hypothetical protein T18N14.20 -
Arabidopsis thaliana, partial (10%)
Length = 394
Score = 26.6 bits (57), Expect = 3.8
Identities = 22/58 (37%), Positives = 34/58 (57%), Gaps = 1/58 (1%)
Frame = +2
Query: 48 EEERKEKRAAQKAAIEGFKQLLDEASE-DIDDKTDSHTFRKKWGNDPRFEALDRKERE 104
EEERK++ K +E ++L DE E + D+ SHT ++K D R EA ++K +E
Sbjct: 59 EEERKQREEVAK-LVEERRRLRDETIEAEKDNSKLSHTSKEK---DRRKEA-EKKRQE 217
>TC85996 weakly similar to GP|16648871|gb|AAL24287.1 Unknown protein
{Arabidopsis thaliana}, partial (53%)
Length = 1446
Score = 26.6 bits (57), Expect = 3.8
Identities = 8/23 (34%), Positives = 13/23 (55%)
Frame = -3
Query: 121 NSLNFIFFVCDSHNHHPYHWITY 143
N ++IF++ H HP H T+
Sbjct: 1411 NKTSYIFYIVQHHREHPTHTYTF 1343
>BE248698 weakly similar to GP|12836661|db H6 homeo box 3~data source:MGD
source key:MGI:107160 evidence:ISS~putative {Mus
musculus}, partial (3%)
Length = 416
Score = 26.6 bits (57), Expect = 3.8
Identities = 11/26 (42%), Positives = 18/26 (68%)
Frame = +2
Query: 48 EEERKEKRAAQKAAIEGFKQLLDEAS 73
EEE++E + ++ +EG K+ LDE S
Sbjct: 242 EEEQEEWKETEEKVVEGLKEELDEFS 319
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.322 0.137 0.437
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 5,746,760
Number of Sequences: 36976
Number of extensions: 85640
Number of successful extensions: 727
Number of sequences better than 10.0: 47
Number of HSP's better than 10.0 without gapping: 720
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 727
length of query: 146
length of database: 9,014,727
effective HSP length: 87
effective length of query: 59
effective length of database: 5,797,815
effective search space: 342071085
effective search space used: 342071085
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 54 (25.4 bits)
Medicago: description of AC144765.19