BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC144765.14 - phase: 0
(113 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC85559 similar to PIR|T07012|T07012 acetyl-CoA carboxylase (EC ... 30 0.18
AW696089 homologue to GP|1865721|emb| mitochondrial single-subun... 28 0.69
TC85259 homologue to PIR|T09260|T09260 aquaporin-like transmembr... 28 0.69
TC85265 similar to GP|10177300|dbj|BAB10561. topoisomerase-like ... 28 0.69
TC80990 similar to PIR|A86224|A86224 hypothetical protein [impor... 27 0.90
TC88314 similar to GP|8843774|dbj|BAA97322.1 gene_id:MYN8.6~simi... 27 1.5
TC77956 similar to GP|16323053|gb|AAL15261.1 AT3g01640/F4P13_18 ... 27 1.5
AW299043 weakly similar to PIR|T00864|T00 cytochrome P450 homolo... 26 2.0
TC85835 similar to PIR|T12180|T12180 probable transcription fact... 26 2.6
TC80084 similar to GP|15450619|gb|AAK96581.1 AT3g14110/MAG2_6 {A... 26 2.6
TC77330 similar to GP|13161397|dbj|BAB33033. CPRD2 {Vigna unguic... 26 2.6
TC89975 similar to PIR|G96530|G96530 probable acyl CoA synthetas... 26 2.6
TC77329 similar to GP|13161397|dbj|BAB33033. CPRD2 {Vigna unguic... 25 4.5
TC80571 similar to GP|22136852|gb|AAM91770.1 unknown protein {Ar... 25 4.5
TC88344 similar to GP|22093702|dbj|BAC06996. contains ESTs AU091... 25 4.5
BQ139850 25 5.8
BI267745 homologue to GP|15982743|gb AT5g17920/MPI7_60 {Arabidop... 25 5.8
BM814761 similar to GP|16604571|gb unknown protein {Arabidopsis ... 25 5.8
BQ135381 25 5.8
TC81852 25 5.8
>TC85559 similar to PIR|T07012|T07012 acetyl-CoA carboxylase (EC 6.4.1.2) -
potato chloroplast, partial (66%)
Length = 5011
Score = 29.6 bits (65), Expect = 0.18
Identities = 12/33 (36%), Positives = 20/33 (60%)
Frame = +2
Query: 7 ERAGDSYWGKKSKAASLRATAIRLHDLNLEDAN 39
+R GD +W + SK L + + +HD++LE N
Sbjct: 1796 KR*GDGFWSRYSKVRILPSQVMDIHDISLEYKN 1894
>AW696089 homologue to GP|1865721|emb| mitochondrial single-subunit
DNA-dependent RNA polymerase {Chenopodium album},
partial (2%)
Length = 615
Score = 27.7 bits (60), Expect = 0.69
Identities = 25/85 (29%), Positives = 37/85 (43%), Gaps = 9/85 (10%)
Frame = +3
Query: 14 WGKKSKAASLR--------ATAIRL-HDLNLEDANAILREAAEIFEGLGMVDSAAQCFTD 64
W K +K AS R +T++R H N + ILR F +G+ S
Sbjct: 138 WSKLAKRASSRRFILPSQSSTSLRFSHQHNFPEK--ILRHPFSRFSQVGIFTS------- 290
Query: 65 LGDYERAGINFNFGIHVYSFMSFSI 89
+Y+ NF+F H +SF S S+
Sbjct: 291 --EYKLGRSNFSFSEHPFSFSSISL 359
>TC85259 homologue to PIR|T09260|T09260 aquaporin-like transmembrane channel
protein - alfalfa, complete
Length = 1502
Score = 27.7 bits (60), Expect = 0.69
Identities = 13/32 (40%), Positives = 18/32 (55%)
Frame = +2
Query: 35 LEDANAILREAAEIFEGLGMVDSAAQCFTDLG 66
+EDA + + E +E G+V A QC DLG
Sbjct: 1406 VEDAKDKITKLLEEYESGGVVGEACQCIRDLG 1501
>TC85265 similar to GP|10177300|dbj|BAB10561. topoisomerase-like protein
{Arabidopsis thaliana}, partial (56%)
Length = 1803
Score = 27.7 bits (60), Expect = 0.69
Identities = 13/32 (40%), Positives = 18/32 (55%)
Frame = +3
Query: 35 LEDANAILREAAEIFEGLGMVDSAAQCFTDLG 66
+EDA + + E +E G+V A QC DLG
Sbjct: 936 VEDAKDKITKLLEEYESGGVVGEACQCIRDLG 1031
>TC80990 similar to PIR|A86224|A86224 hypothetical protein [imported] -
Arabidopsis thaliana, partial (71%)
Length = 1343
Score = 27.3 bits (59), Expect = 0.90
Identities = 15/42 (35%), Positives = 23/42 (54%)
Frame = +1
Query: 4 MCFERAGDSYWGKKSKAASLRATAIRLHDLNLEDANAILREA 45
M F++ G+++W + L +LH L + DA ILREA
Sbjct: 502 MEFQKKGETFWNDSYRL*LLMDGL*QLHLLGIPDAY*ILREA 627
>TC88314 similar to GP|8843774|dbj|BAA97322.1 gene_id:MYN8.6~similar to
unknown protein~sp|P29618 {Arabidopsis thaliana}, partial
(71%)
Length = 2143
Score = 26.6 bits (57), Expect = 1.5
Identities = 10/27 (37%), Positives = 13/27 (48%)
Frame = -3
Query: 80 HVYSFMSFSIKKHYDILEHKYQVYPTW 106
H + F +K H D L H+Y TW
Sbjct: 2006 HFEQYQCFDLKDHRDSLSHQYHPT*TW 1926
>TC77956 similar to GP|16323053|gb|AAL15261.1 AT3g01640/F4P13_18 {Arabidopsis
thaliana}, partial (90%)
Length = 1412
Score = 26.6 bits (57), Expect = 1.5
Identities = 10/22 (45%), Positives = 16/22 (72%)
Frame = -3
Query: 77 FGIHVYSFMSFSIKKHYDILEH 98
+ HV+SF++ SIKKH +E+
Sbjct: 1296 YSTHVFSFLNSSIKKHESHIEN 1231
>AW299043 weakly similar to PIR|T00864|T00 cytochrome P450 homolog F17K2.4 -
Arabidopsis thaliana, partial (34%)
Length = 594
Score = 26.2 bits (56), Expect = 2.0
Identities = 10/30 (33%), Positives = 15/30 (49%)
Frame = -2
Query: 77 FGIHVYSFMSFSIKKHYDILEHKYQVYPTW 106
F IHV F HYD+++ Y++ W
Sbjct: 170 FSIHVQYFSHLLCTTHYDLVQEVYKLDEFW 81
>TC85835 similar to PIR|T12180|T12180 probable transcription factor - fava
bean, partial (79%)
Length = 1737
Score = 25.8 bits (55), Expect = 2.6
Identities = 15/51 (29%), Positives = 24/51 (46%)
Frame = +3
Query: 61 CFTDLGDYERAGINFNFGIHVYSFMSFSIKKHYDILEHKYQVYPTWSYIVF 111
CF LG Y +V+SF+ F + +L +Q+YP+ Y+ F
Sbjct: 1551 CFDILGGYMVVDKT-----NVFSFVLFYRLNEFSVLL*VFQIYPSLMYVGF 1688
>TC80084 similar to GP|15450619|gb|AAK96581.1 AT3g14110/MAG2_6 {Arabidopsis
thaliana}, partial (63%)
Length = 1123
Score = 25.8 bits (55), Expect = 2.6
Identities = 9/14 (64%), Positives = 12/14 (85%)
Frame = +2
Query: 59 AQCFTDLGDYERAG 72
A C+T+LGD E+AG
Sbjct: 902 ADCYTELGDLEKAG 943
>TC77330 similar to GP|13161397|dbj|BAB33033. CPRD2 {Vigna unguiculata},
partial (29%)
Length = 1295
Score = 25.8 bits (55), Expect = 2.6
Identities = 11/27 (40%), Positives = 13/27 (47%)
Frame = -2
Query: 73 INFNFGIHVYSFMSFSIKKHYDILEHK 99
I F H+Y M F I K Y + HK
Sbjct: 523 IEFCIRFHIYVEMEFQILKSYPLCHHK 443
>TC89975 similar to PIR|G96530|G96530 probable acyl CoA synthetase
[imported] - Arabidopsis thaliana, partial (46%)
Length = 1251
Score = 25.8 bits (55), Expect = 2.6
Identities = 9/18 (50%), Positives = 13/18 (72%)
Frame = +1
Query: 50 EGLGMVDSAAQCFTDLGD 67
+G G+ +S A CFT +GD
Sbjct: 337 QGYGLTESCAGCFTAIGD 390
>TC77329 similar to GP|13161397|dbj|BAB33033. CPRD2 {Vigna unguiculata},
partial (13%)
Length = 489
Score = 25.0 bits (53), Expect = 4.5
Identities = 10/30 (33%), Positives = 14/30 (46%)
Frame = -1
Query: 75 FNFGIHVYSFMSFSIKKHYDILEHKYQVYP 104
F+ HV M F K+Y + HK + P
Sbjct: 330 FDINFHVCVEMEFQTLKYYPLCHHKENISP 241
>TC80571 similar to GP|22136852|gb|AAM91770.1 unknown protein {Arabidopsis
thaliana}, partial (35%)
Length = 1502
Score = 25.0 bits (53), Expect = 4.5
Identities = 11/36 (30%), Positives = 20/36 (55%)
Frame = -2
Query: 76 NFGIHVYSFMSFSIKKHYDILEHKYQVYPTWSYIVF 111
N + Y + SI ++ + H+Y +Y WS+I+F
Sbjct: 1231 NTSVLPY*WSFASILRYNCTVGHQYYLYCHWSFIIF 1124
>TC88344 similar to GP|22093702|dbj|BAC06996. contains ESTs AU091644(C1340)
D22794(C1340)~unknown protein {Oryza sativa (japonica
cultivar-group)}, partial (44%)
Length = 565
Score = 25.0 bits (53), Expect = 4.5
Identities = 17/65 (26%), Positives = 27/65 (41%)
Frame = +1
Query: 28 IRLHDLNLEDANAILREAAEIFEGLGMVDSAAQCFTDLGDYERAGINFNFGIHVYSFMSF 87
++LH L L++ + I E L + D CF + ++ F +H F S
Sbjct: 220 LQLHFL*LDEGSIICHME*ERRNDLTISDGGGGCFATQTEQNLLMMDLRFSLH--PFFSL 393
Query: 88 SIKKH 92
IK H
Sbjct: 394 LIKSH 408
>BQ139850
Length = 356
Score = 24.6 bits (52), Expect = 5.8
Identities = 8/14 (57%), Positives = 13/14 (92%)
Frame = +1
Query: 77 FGIHVYSFMSFSIK 90
FG+ VYS+++FS+K
Sbjct: 175 FGVDVYSYLTFSLK 216
>BI267745 homologue to GP|15982743|gb AT5g17920/MPI7_60 {Arabidopsis
thaliana}, partial (24%)
Length = 657
Score = 24.6 bits (52), Expect = 5.8
Identities = 10/25 (40%), Positives = 15/25 (60%)
Frame = +2
Query: 11 DSYWGKKSKAASLRATAIRLHDLNL 35
+S+W KKS A L+ + + LNL
Sbjct: 149 ESFWDKKSSAEDLKKVSAQQKKLNL 223
>BM814761 similar to GP|16604571|gb unknown protein {Arabidopsis thaliana},
partial (53%)
Length = 674
Score = 24.6 bits (52), Expect = 5.8
Identities = 9/14 (64%), Positives = 9/14 (64%)
Frame = +3
Query: 5 CFERAGDSYWGKKS 18
CF GDSYWG S
Sbjct: 54 CFMGYGDSYWGASS 95
>BQ135381
Length = 1279
Score = 24.6 bits (52), Expect = 5.8
Identities = 9/32 (28%), Positives = 15/32 (46%)
Frame = +2
Query: 3 TMCFERAGDSYWGKKSKAASLRATAIRLHDLN 34
T+C ++W + RAT R+H +N
Sbjct: 998 TVCTTHTSRAWWWGCMRGREARATVTRIHSIN 1093
>TC81852
Length = 527
Score = 24.6 bits (52), Expect = 5.8
Identities = 9/24 (37%), Positives = 13/24 (53%)
Frame = +3
Query: 90 KKHYDILEHKYQVYPTWSYIVFIC 113
K H + +++V P W IV IC
Sbjct: 57 KSHEEAKRFRFKVIPNWDDIVAIC 128
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.325 0.138 0.428
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 3,649,355
Number of Sequences: 36976
Number of extensions: 39664
Number of successful extensions: 285
Number of sequences better than 10.0: 45
Number of HSP's better than 10.0 without gapping: 284
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 285
length of query: 113
length of database: 9,014,727
effective HSP length: 89
effective length of query: 24
effective length of database: 5,723,863
effective search space: 137372712
effective search space used: 137372712
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)
S2: 50 (23.9 bits)
Medicago: description of AC144765.14