BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC144765.13 + phase: 0
(146 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BE941067 weakly similar to GP|6648198|gb| unknown protein {Arabi... 221 6e-59
CB892916 similar to GP|6648198|gb|A unknown protein {Arabidopsis... 127 2e-30
BG451797 similar to GP|18028626|gb| maturase {Zippelia begoniifo... 64 2e-11
BE941307 homologue to GP|21280323|dbj AGO1 homologous protein {O... 27 2.9
TC85231 similar to GP|13508844|emb|CAC35167. arbutin synthase {R... 27 3.8
AW682962 similar to GP|8777328|dbj| ABC transporter-like protein... 27 3.8
TC80272 similar to GP|15620810|dbj|BAB67768. RPS6-like protein {... 26 6.5
TC80043 similar to GP|9759561|dbj|BAB11163.1 MtN21 nodulin prote... 26 6.5
TC85916 similar to GP|9758968|dbj|BAB09411.1 26S proteasome p55 ... 26 6.5
TC79069 weakly similar to GP|9758000|dbj|BAB08422.1 MtN3 protein... 26 6.5
TC88447 similar to GP|21593015|gb|AAM64964.1 unknown {Arabidopsi... 25 8.5
TC87129 weakly similar to PIR|F84503|F84503 hypothetical protein... 25 8.5
>BE941067 weakly similar to GP|6648198|gb| unknown protein {Arabidopsis
thaliana}, partial (59%)
Length = 601
Score = 221 bits (564), Expect = 6e-59
Identities = 112/117 (95%), Positives = 113/117 (95%)
Frame = +2
Query: 16 VLKALLEDPILADVPKNPTLEDVETLIGLELGSAMRITVLKLDTTSFDVILMNTATLKDL 75
VLKA LEDPILADVPKNPTLEDV LIGL LGSAMRITVLKLDTTSFDVILMNTATLKDL
Sbjct: 2 VLKA*LEDPILADVPKNPTLEDVGALIGL*LGSAMRITVLKLDTTSFDVILMNTATLKDL 181
Query: 76 KLAIKKKVNYMEQSSMGHRHISWRSVWANYCLSFDNNKLLNDDDVLQNLGVRNNSQV 132
KLAIKKKVNYMEQSSMGHRHISWRSVWANYCLSFDNNKLLNDDDVLQNLGVRNNSQ+
Sbjct: 182 KLAIKKKVNYMEQSSMGHRHISWRSVWANYCLSFDNNKLLNDDDVLQNLGVRNNSQI 352
>CB892916 similar to GP|6648198|gb|A unknown protein {Arabidopsis thaliana},
partial (25%)
Length = 388
Score = 127 bits (319), Expect = 2e-30
Identities = 59/61 (96%), Positives = 60/61 (97%)
Frame = +2
Query: 72 LKDLKLAIKKKVNYMEQSSMGHRHISWRSVWANYCLSFDNNKLLNDDDVLQNLGVRNNSQ 131
LKDLKLAIKKKVNYMEQSSMGHRHISWRSVWANYCLSFDNNKL NDDDVLQNLGVRNNSQ
Sbjct: 2 LKDLKLAIKKKVNYMEQSSMGHRHISWRSVWANYCLSFDNNKLFNDDDVLQNLGVRNNSQ 181
Query: 132 V 132
+
Sbjct: 182 I 184
>BG451797 similar to GP|18028626|gb| maturase {Zippelia begoniifolia},
partial (2%)
Length = 383
Score = 63.9 bits (154), Expect = 2e-11
Identities = 31/34 (91%), Positives = 31/34 (91%)
Frame = +3
Query: 1 MGEGAEYKSGLKKAKVLKALLEDPILADVPKNPT 34
MGEGAEYKSGLKKAKVLKALLEDPIL VP NPT
Sbjct: 282 MGEGAEYKSGLKKAKVLKALLEDPILXXVPXNPT 383
>BE941307 homologue to GP|21280323|dbj AGO1 homologous protein {Oryza sativa
(japonica cultivar-group)}, partial (6%)
Length = 619
Score = 26.9 bits (58), Expect = 2.9
Identities = 10/21 (47%), Positives = 14/21 (66%)
Frame = +3
Query: 121 LQNLGVRNNSQVSSTYLCHCW 141
L+ LG+RN S + TY C+ W
Sbjct: 516 LELLGIRNKSHMYKTYSCY*W 578
>TC85231 similar to GP|13508844|emb|CAC35167. arbutin synthase {Rauvolfia
serpentina}, partial (34%)
Length = 1602
Score = 26.6 bits (57), Expect = 3.8
Identities = 17/59 (28%), Positives = 29/59 (48%)
Frame = -3
Query: 13 KAKVLKALLEDPILADVPKNPTLEDVETLIGLELGSAMRITVLKLDTTSFDVILMNTAT 71
KA+ A LE+P PTL + + +L S I + L T+S +I ++++T
Sbjct: 1432 KAREESAFLEEPCSLSTVAAPTLNSLIFFLISKLSSPFMILFIALATSSCFIIPVSSST 1256
>AW682962 similar to GP|8777328|dbj| ABC transporter-like protein
{Arabidopsis thaliana}, partial (7%)
Length = 631
Score = 26.6 bits (57), Expect = 3.8
Identities = 16/55 (29%), Positives = 25/55 (45%), Gaps = 4/55 (7%)
Frame = +2
Query: 52 ITVLKLDTTSFDVILMNTATLK----DLKLAIKKKVNYMEQSSMGHRHISWRSVW 102
IT +K T + DV + L DLK AI ++ + S+ ++ W S W
Sbjct: 245 ITNVKRSTHNMDVCKRKSCVLSYILTDLKYAILLACHWCDDCSLNSCNLIWTSTW 409
>TC80272 similar to GP|15620810|dbj|BAB67768. RPS6-like protein {Arabidopsis
thaliana}, partial (35%)
Length = 1026
Score = 25.8 bits (55), Expect = 6.5
Identities = 12/53 (22%), Positives = 26/53 (48%), Gaps = 1/53 (1%)
Frame = +1
Query: 55 LKLDTTSFDVILMNTATLKDLKLAIKKKVNYMEQSSMGHRHISWRSVWA-NYC 106
+K +S ++ + L+ + + M++SSM ++W+ +W NYC
Sbjct: 229 MKPPASSLNLFFLKRRQLRRMVNFFRSLKMMMKESSMNP*SLTWKLIWTLNYC 387
>TC80043 similar to GP|9759561|dbj|BAB11163.1 MtN21 nodulin protein-like
{Arabidopsis thaliana}, partial (28%)
Length = 1421
Score = 25.8 bits (55), Expect = 6.5
Identities = 14/32 (43%), Positives = 17/32 (52%), Gaps = 5/32 (15%)
Frame = +1
Query: 89 SSMGHRH-----ISWRSVWANYCLSFDNNKLL 115
S+ GH H ISWR W+N FD N+ L
Sbjct: 331 SNYGHFHPCRKIISWRCYWSN----FDRNRTL 414
>TC85916 similar to GP|9758968|dbj|BAB09411.1 26S proteasome p55
protein-like {Arabidopsis thaliana}, partial (95%)
Length = 1807
Score = 25.8 bits (55), Expect = 6.5
Identities = 31/135 (22%), Positives = 57/135 (41%), Gaps = 9/135 (6%)
Frame = +2
Query: 11 LKKAKVLKALLEDPILADVPKN--PTLEDVETLIGLELGSAMRITVLKLDTTSFDVILMN 68
L+ + KA+ E P + + P + P L + + L S M+ ++L
Sbjct: 746 LEICRCYKAIYEIPSIKENPADFIPILRKICWCLVLAPHSPMQSSLLN------------ 889
Query: 69 TATLKDLKLAIKKKVNYMEQSSMGHRHISWRSVWANYCLSFDN----NKLLND---DDVL 121
+TL+D L+ + + + I W ++W Y FDN K L++ +D+
Sbjct: 890 -STLEDRNLSEIPNFQLLLKQLVTMEVIQWTALWETYKNEFDNEMVSGKFLSEKAAEDLK 1066
Query: 122 QNLGVRNNSQVSSTY 136
Q + + +N V S Y
Sbjct: 1067QRI-IEHNILVVSKY 1108
>TC79069 weakly similar to GP|9758000|dbj|BAB08422.1 MtN3 protein-like
{Arabidopsis thaliana}, partial (73%)
Length = 1038
Score = 25.8 bits (55), Expect = 6.5
Identities = 20/63 (31%), Positives = 35/63 (54%)
Frame = +3
Query: 18 KALLEDPILADVPKNPTLEDVETLIGLELGSAMRITVLKLDTTSFDVILMNTATLKDLKL 77
K ++E+P D+PK +LE+++ LE G + + K TTS ++ N ++DL+L
Sbjct: 684 KVVIEEPNKVDLPKLVSLENLD----LEKGGLEKGNLEKNVTTS*VMMCNN---VQDLRL 842
Query: 78 AIK 80
K
Sbjct: 843 VAK 851
>TC88447 similar to GP|21593015|gb|AAM64964.1 unknown {Arabidopsis
thaliana}, partial (47%)
Length = 1079
Score = 25.4 bits (54), Expect = 8.5
Identities = 14/35 (40%), Positives = 17/35 (48%)
Frame = -1
Query: 86 MEQSSMGHRHISWRSVWANYCLSFDNNKLLNDDDV 120
+ SS H H S A Y LS D+N L DD+
Sbjct: 470 LSSSSSYHGHDLCLSSRAPYLLSHDHNPFLPSDDI 366
>TC87129 weakly similar to PIR|F84503|F84503 hypothetical protein At2g12550
[imported] - Arabidopsis thaliana, partial (63%)
Length = 1894
Score = 25.4 bits (54), Expect = 8.5
Identities = 11/26 (42%), Positives = 18/26 (68%), Gaps = 3/26 (11%)
Frame = +1
Query: 113 KLLNDDD---VLQNLGVRNNSQVSST 135
++L DDD L LG++NNS++ +T
Sbjct: 187 RILKDDDKTLTLTQLGIKNNSKILAT 264
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.317 0.133 0.390
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 4,579,015
Number of Sequences: 36976
Number of extensions: 58008
Number of successful extensions: 449
Number of sequences better than 10.0: 24
Number of HSP's better than 10.0 without gapping: 447
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 449
length of query: 146
length of database: 9,014,727
effective HSP length: 87
effective length of query: 59
effective length of database: 5,797,815
effective search space: 342071085
effective search space used: 342071085
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 54 (25.4 bits)
Medicago: description of AC144765.13