BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC144765.10 - phase: 0
(552 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC86737 weakly similar to GP|6683624|dbj|BAA89272.1 Pol {Alterna... 301 6e-82
BG644693 weakly similar to GP|18767374|g Putative 22 kDa kafirin... 224 5e-59
BG586326 similar to PIR|G84493|G8 probable retroelement pol poly... 148 5e-36
BG587145 similar to PIR|H86337|H8 protein F5M15.26 [imported] - ... 93 3e-31
BG644740 similar to PIR|A84460|A84 probable retroelement pol pol... 88 1e-26
CA860311 weakly similar to GP|7289872|gb|A CG17427 gene product ... 41 0.001
AJ497987 weakly similar to GP|9927273|dbj Similar to Arabidopsis... 35 0.098
CB893783 weakly similar to GP|22830935|dbj hypothetical protein~... 33 0.37
BG646119 weakly similar to PIR|T46196|T46 cytochrome P450-like p... 32 0.63
TC78233 similar to GP|15809838|gb|AAL06847.1 AT5g51120/MWD22_6 {... 30 1.8
BF643894 29 5.4
TC89026 similar to GP|8809658|dbj|BAA97209.1 gb|AAD55296.1~gene_... 28 7.0
>TC86737 weakly similar to GP|6683624|dbj|BAA89272.1 Pol {Alternaria
alternata}, partial (21%)
Length = 1540
Score = 301 bits (770), Expect = 6e-82
Identities = 168/390 (43%), Positives = 240/390 (61%), Gaps = 14/390 (3%)
Frame = +1
Query: 117 VVNEFHEVF-PDEIPDVPPEREV-EFSIDLVPGAK-----LVSMAPYHMSASELAELKKQ 169
V+ EF ++F P++ VP R + + +I L+P L Y MS EL LKK
Sbjct: 343 VLEEFPDLFNPEKAYQVPASRGLLDHAIPLIPDKDGNDPPLPWGPLYGMSRQELLVLKKT 522
Query: 170 LEDLLDKKFVRPSVSPWGAPVLLVKKKDGSMRLCIDYRQLNKVTIKNRYPLPRIDDLMDQ 229
LEDLLDK F++ S S GAPVL V+K G +R C+DYR LN +T K+RYPLP I + + +
Sbjct: 523 LEDLLDKGFIKASGSAAGAPVLFVRKPGGGIRFCVDYRALNAITKKDRYPLPLISETLRR 702
Query: 230 LVGAKVFSKIDLRSGYHQIKVKDEDMQKTAFRTRYGHYEYKVMPFGVTNAPGVFMEYMNR 289
+ GA+ F+K+D+ + +H++++KDED +KTAFRTRYG +E+ V PFG+T AP F Y+N+
Sbjct: 703 VAGARWFTKLDVVAAFHKMRIKDEDQEKTAFRTRYGLFEWIVCPFGLTGAPATFQRYINK 882
Query: 290 IFHAFLDKFVVVFIDDILIYSK-IEEEHAKHLKIVLQILKERKLYAKLSKCEFWLSEVSF 348
H FLD FV +IDD+LIY+ +++H ++ VL+ L + L KCEF ++ V +
Sbjct: 883 TLHEFLDDFVTAYIDDVLIYTTGSKKDHEAQVRRVLRRLADAGLSLDPKKCEFSVTTVKY 1062
Query: 349 LGHV-TSGNGIAVDPSKVDAVSQWETPKSVTEIRSFLGLAGYYRRFIEGFSKLALPLTQL 407
+G + T+G G++ DP K+ A+ W P SV RSFLG YY+ FI G+S++ PLT+L
Sbjct: 1063VGFILTAGKGVSCDPLKLAAIRDWLPPGSVKGARSFLGFCNYYKDFIPGYSEITEPLTRL 1242
Query: 408 TYKGKSFVWDAQCESSFNELKQRLTTAPILILPKPEEPFVVYCDASKFGLGGVLMQD--- 464
T K F W A+ E++F +LK+ P+L + PE V D S F LGGVL Q+
Sbjct: 1243TRKDFPFRWGAEQEAAFTKLKRLFAEEPVLRMFDPEAVTTVETDCSGFALGGVLTQEDGT 1422
Query: 465 --GKVVAYASRQLRVHEKNSPTHDLELAAV 492
VA+ S++L E N P HD EL AV
Sbjct: 1423GAAHPVAFHSQRLSPAEYNYPIHDKELLAV 1512
>BG644693 weakly similar to GP|18767374|g Putative 22 kDa kafirin cluster;
Ty3-Gypsy type {Oryza sativa}, partial (15%)
Length = 716
Score = 224 bits (572), Expect = 5e-59
Identities = 122/243 (50%), Positives = 158/243 (64%), Gaps = 5/243 (2%)
Frame = +2
Query: 127 DEIPDVPPEREVEFSIDLVPGAKLVSMAPYHMSASELAELKKQLEDLLDKKFVRPSVSPW 186
D + VPPE +++F IDL+P + + Y ++ +L LK QL+DLL+K F++PS+ P
Sbjct: 2 DHLL*VPPEWKIDFGIDLLPNMNPI*IPSYRINPLKLKVLKLQLKDLLEKGFIQPSIYP* 181
Query: 187 GAPVLLVKKKDGSMRLCIDYRQLNKVTIKNRYPLPRIDDLMDQLVGAKVFSKIDLRSGYH 246
G VL +KKKDG +R+ IDY QLN V IK +YPLP ID+L D L G+K F KIDLR G H
Sbjct: 182 GVVVLFLKKKDGFLRMSIDYPQLNNVNIKIKYPLPLIDELFDNLQGSKWFFKIDLRLG*H 361
Query: 247 QIKVKDEDMQKTAFRTRYGHYEYKVMPFGVTNAPGVFMEYMNRIFHAFLDKFVVVFIDDI 306
Q +V ED+ KTAFR RYGHYE VM FG TN P FME MNR+F +LD V+VF +DI
Sbjct: 362 QHRVIGEDVPKTAFRIRYGHYEILVMSFG*TNPPMAFMELMNRVFQDYLDSLVIVFSNDI 541
Query: 307 LIYSKIEEEHAKHLKIVLQILKERKLYAKLSKCEF----WLSEVSFLG-HVTSGNGIAVD 361
LIYSK E EH HL++ L++LK+ + C+ L EV F HV SG G+ VD
Sbjct: 542 LIYSKNENEHENHLRLALKVLKD------IGLCQISYV*ILVEVGFFSLHVISGEGLKVD 703
Query: 362 PSK 364
+
Sbjct: 704 SKR 712
>BG586326 similar to PIR|G84493|G8 probable retroelement pol polyprotein
[imported] - Arabidopsis thaliana, partial (13%)
Length = 736
Score = 148 bits (374), Expect = 5e-36
Identities = 73/110 (66%), Positives = 88/110 (79%)
Frame = +2
Query: 432 TTAPILILPKPEEPFVVYCDASKFGLGGVLMQDGKVVAYASRQLRVHEKNSPTHDLELAA 491
T+APIL+LP+ +VVY DAS GLG VL Q KV+AYASRQLR HE N PTHDLE+AA
Sbjct: 8 TSAPILVLPELIT-YVVYTDASITGLGCVLTQHEKVIAYASRQLRKHEGNYPTHDLEMAA 184
Query: 492 VVFVLKIWRHYLYGSRFEVFSDHKSLKYLFDQKELNMRQRRWLELLKDYD 541
VVF LKIWR YLYG++ ++ +DHKSLKY+F Q ELN+RQRRW+E + DYD
Sbjct: 185 VVFALKIWRSYLYGAKVQIHTDHKSLKYIFTQPELNLRQRRWMEFVADYD 334
>BG587145 similar to PIR|H86337|H8 protein F5M15.26 [imported] - Arabidopsis
thaliana, partial (13%)
Length = 763
Score = 93.2 bits (230), Expect(2) = 3e-31
Identities = 48/133 (36%), Positives = 76/133 (57%)
Frame = +2
Query: 253 EDMQKTAFRTRYGHYEYKVMPFGVTNAPGVFMEYMNRIFHAFLDKFVVVFIDDILIYSKI 312
+D++KTAF T G Y YKVMPFG+ NA + +NR+F L + V+IDD+L+ S
Sbjct: 11 DDLEKTAFITDRGTYCYKVMPFGLKNAGSTYQRLVNRMFADKLGNTMEVYIDDMLVKSLR 190
Query: 313 EEEHAKHLKIVLQILKERKLYAKLSKCEFWLSEVSFLGHVTSGNGIAVDPSKVDAVSQWE 372
+H HLK + L E + +KC F ++ FLG++ + GI V+P ++ A+
Sbjct: 191 ATDHLNHLKE*FKTLDEYIMKLNPAKCTFGVTSGEFLGYIVTQQGIEVNPKQITAILDLP 370
Query: 373 TPKSVTEIRSFLG 385
+PK+ E++ G
Sbjct: 371 SPKNSREVQRLTG 409
Score = 60.5 bits (145), Expect(2) = 3e-31
Identities = 35/106 (33%), Positives = 54/106 (50%), Gaps = 4/106 (3%)
Frame = +3
Query: 392 RFIEGFSKLALPLTQLTYKGKSFVWDAQCESSFNELKQRLTTAPILILPKPEEPFVVYCD 451
RFI + LP +L K FVWD +CE +F +LKQ LTT P+L P+ + +Y
Sbjct: 429 RFISRSTDKCLPFYKLLCGNKRFVWDEKCEEAFEQLKQYLTTPPVLSKPEAGDTLSLYIA 608
Query: 452 ASKFGLGGVLMQDG----KVVAYASRQLRVHEKNSPTHDLELAAVV 493
S + VL+++ K + Y S+++ E PT + AV+
Sbjct: 609 ISSTAVSSVLIREDRGEQKPIFYTSKRMTDPETRYPTLEKMAFAVI 746
>BG644740 similar to PIR|A84460|A84 probable retroelement pol polyprotein
[imported] - Arabidopsis thaliana, partial (4%)
Length = 754
Score = 88.2 bits (217), Expect(2) = 1e-26
Identities = 42/69 (60%), Positives = 52/69 (74%)
Frame = -1
Query: 176 KKFVRPSVSPWGAPVLLVKKKDGSMRLCIDYRQLNKVTIKNRYPLPRIDDLMDQLVGAKV 235
K+F +PS+SP GA +L V+KKDG R+CIDYRQ NKVT KN+YPLPRID+L D++
Sbjct: 274 KRFQQPSISP*GAALLFVRKKDGYFRMCIDYRQFNKVTTKNKYPLPRIDNLFDKIQEDCY 95
Query: 236 FSKIDLRSG 244
F IDLR G
Sbjct: 94 F*NIDLRLG 68
Score = 50.1 bits (118), Expect(2) = 1e-26
Identities = 28/62 (45%), Positives = 38/62 (61%)
Frame = -2
Query: 117 VVNEFHEVFPDEIPDVPPEREVEFSIDLVPGAKLVSMAPYHMSASELAELKKQLEDLLDK 176
V+ F VFPD P +P ERE+ F IDL+ +L+S P M +EL +LK L+D L+K
Sbjct: 450 VLKGFS*VFPDNFPVIPLEREIFFCIDLLLDTQLISNPP*LMDRTELKKLKI*LKDSLEK 271
Query: 177 KF 178
F
Sbjct: 270 GF 265
>CA860311 weakly similar to GP|7289872|gb|A CG17427 gene product {Drosophila
melanogaster}, partial (20%)
Length = 192
Score = 40.8 bits (94), Expect = 0.001
Identities = 22/64 (34%), Positives = 36/64 (55%), Gaps = 5/64 (7%)
Frame = +1
Query: 452 ASKFGLGGVLMQDGKV-----VAYASRQLRVHEKNSPTHDLELAAVVFVLKIWRHYLYGS 506
A +G+G L Q + +AYASR L E+N + E A ++ ++ +RHYL+G
Sbjct: 1 APDWGIGAGLSQKDEENHEHPIAYASRLLTAAERNYTVVERECLAAIWAIRNFRHYLHGP 180
Query: 507 RFEV 510
+FE+
Sbjct: 181 KFEL 192
>AJ497987 weakly similar to GP|9927273|dbj Similar to Arabidopsis thaliana
chromosome II BAC F26H6; putative retroelement pol
polyprotein, partial (1%)
Length = 636
Score = 34.7 bits (78), Expect = 0.098
Identities = 17/51 (33%), Positives = 26/51 (50%)
Frame = -2
Query: 491 AVVFVLKIWRHYLYGSRFEVFSDHKSLKYLFDQKELNMRQRRWLELLKDYD 541
A+ + K RHY+ + S +KY+F++ L R RW LL +YD
Sbjct: 623 ALAWAAKRLRHYMINHTTWLVSKMDPIKYIFEKPALTGRIARWQMLLSEYD 471
>CB893783 weakly similar to GP|22830935|dbj hypothetical protein~similar to
gag-pol polyprotein {Oryza sativa (japonica
cultivar-group)}, partial (8%)
Length = 853
Score = 32.7 bits (73), Expect = 0.37
Identities = 13/29 (44%), Positives = 21/29 (71%), Gaps = 1/29 (3%)
Frame = +2
Query: 120 EFHEVFPDEIPD-VPPEREVEFSIDLVPG 147
+F +V P EIP +PP R+++ +ID +PG
Sbjct: 764 DFTDVVPSEIPSGLPPMRDIQHAIDFIPG 850
>BG646119 weakly similar to PIR|T46196|T46 cytochrome P450-like protein -
Arabidopsis thaliana, partial (37%)
Length = 712
Score = 32.0 bits (71), Expect = 0.63
Identities = 18/55 (32%), Positives = 29/55 (52%)
Frame = -1
Query: 99 SLMASLSLENQVVIDRLPVVNEFHEVFPDEIPDVPPEREVEFSIDLVPGAKLVSM 153
S+ A+ SL + VV LP++ F FP + P+R + S+ +P KLV +
Sbjct: 469 SVFATKSLRDLVVNSWLPILRCFDHNFPSTLNMPQPKRSLRISVKGLPLGKLVKL 305
>TC78233 similar to GP|15809838|gb|AAL06847.1 AT5g51120/MWD22_6 {Arabidopsis
thaliana}, partial (66%)
Length = 1183
Score = 30.4 bits (67), Expect = 1.8
Identities = 17/61 (27%), Positives = 34/61 (54%)
Frame = +3
Query: 113 DRLPVVNEFHEVFPDEIPDVPPEREVEFSIDLVPGAKLVSMAPYHMSASELAELKKQLED 172
DR+ + E HEV+ +IPD + + + + G + +A H + EL ++KK+L++
Sbjct: 93 DRIAMEQEEHEVYGADIPDEEVDMDADIDAEHQEGDE--ELASNH-TTKELEDMKKRLKE 263
Query: 173 L 173
+
Sbjct: 264 I 266
>BF643894
Length = 591
Score = 28.9 bits (63), Expect = 5.4
Identities = 16/56 (28%), Positives = 27/56 (47%), Gaps = 3/56 (5%)
Frame = -1
Query: 18 SLVCLRCPLSMF---GRDFEVDLVCLPLLGMDVIFGMNWLEYNRVCINCFNKTVHF 70
+L C+ LS F ++ +CL L +D I G N + +C+N N + +F
Sbjct: 534 NLPCMNADLSCF*SWASSVQISYICLQLHKLDPIDGKNTKA*SLICLNSGN*STYF 367
>TC89026 similar to GP|8809658|dbj|BAA97209.1
gb|AAD55296.1~gene_id:MRG21.3~strong similarity to
unknown protein {Arabidopsis thaliana}, partial (18%)
Length = 800
Score = 28.5 bits (62), Expect = 7.0
Identities = 22/62 (35%), Positives = 26/62 (41%), Gaps = 5/62 (8%)
Frame = +1
Query: 406 QLTYKGKSFVWDAQCESSFNELKQRLTTAPILILPKPEE-----PFVVYCDASKFGLGGV 460
QL+ K VW NEL+ T PI PKPE+ P V S+F G
Sbjct: 337 QLSVKDGKRVW--------NELQSAKTQTPIQTAPKPEKRPGSCPRSVSVTGSEFAANGS 492
Query: 461 LM 462
LM
Sbjct: 493 LM 498
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.323 0.140 0.418
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 17,024,849
Number of Sequences: 36976
Number of extensions: 232729
Number of successful extensions: 1142
Number of sequences better than 10.0: 24
Number of HSP's better than 10.0 without gapping: 1131
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1141
length of query: 552
length of database: 9,014,727
effective HSP length: 101
effective length of query: 451
effective length of database: 5,280,151
effective search space: 2381348101
effective search space used: 2381348101
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.5 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (22.0 bits)
S2: 61 (28.1 bits)
Medicago: description of AC144765.10