BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC144731.7 + phase: 0
(228 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC90799 similar to GP|7672991|gb|AAF66690.1| MADS-box transcript... 82 2e-16
TC78724 similar to GP|4322475|gb|AAD16052.1| putative MADS box t... 79 1e-15
TC78994 similar to SP|O64645|SOC1_ARATH SUPPRESSOR OF CONSTANS O... 78 3e-15
BE124909 similar to GP|4322475|gb| putative MADS box transcripti... 78 3e-15
TC86876 similar to GP|16973296|emb|CAC80857. C-type MADS box pro... 75 2e-14
TC87621 similar to SP|Q9FVC1|SVP_ARATH SHORT VEGETATIVE PHASE pr... 75 2e-14
TC81292 homologue to PIR|T09700|T09700 MADS-box protein - alfalf... 74 5e-14
TC82227 similar to GP|1483232|emb|CAA67969.1 MADS5 protein {Betu... 71 3e-13
TC84496 similar to GP|1483232|emb|CAA67969.1 MADS5 protein {Betu... 71 4e-13
AL387855 similar to GP|1483230|emb MADS4 protein {Betula pendula... 70 5e-13
TC93057 homologue to GP|23194453|gb|AAN15183.1 MADS box protein ... 70 7e-13
TC90653 similar to GP|10835358|gb|AAC13695.2 PTD protein {Populu... 70 7e-13
TC82622 similar to GP|21739238|emb|CAD40993. OSJNBa0072F16.17 {O... 69 1e-12
TC84438 homologue to GP|1483232|emb|CAA67969.1 MADS5 protein {Be... 68 3e-12
AW688164 similar to GP|3114588|gb|A MADS box protein {Eucalyptus... 49 2e-08
BI272814 PIR|S71757|S71 MADS box protein DEFH200 - garden snapdr... 49 1e-06
BE999756 weakly similar to GP|9758603|dbj| gene_id:MDF20.13~unkn... 40 0.001
TC91615 weakly similar to GP|21593574|gb|AAM65541.1 unknown {Ara... 32 0.26
TC89605 31 0.34
TC92960 weakly similar to GP|10177030|dbj|BAB10268. contains sim... 29 1.7
>TC90799 similar to GP|7672991|gb|AAF66690.1| MADS-box transcription factor
{Canavalia lineata}, partial (76%)
Length = 653
Score = 81.6 bits (200), Expect = 2e-16
Identities = 52/158 (32%), Positives = 82/158 (50%), Gaps = 3/158 (1%)
Frame = +2
Query: 10 RQKIEMKKISNESNLQVTFSKHHNGLFKKASELCTLCGADVALVVFSPSGKVFSFGHPNL 69
RQKI++KKI N + QVTFSK G+FKKA EL LC A+V LV+FS +GK++ + N+
Sbjct: 77 RQKIKIKKIDNATARQVTFSKRRRGIFKKAEELSILCDAEVGLVIFSTTGKLYEYASSNM 256
Query: 70 DTVIDRFLSLIPTQNDGTMQFIEAHRNANVRELNAQL-TQINNTLDAE--KKIGDELSNL 126
+I R+ ++ ++ +L+ L Q+ + AE K++ D L
Sbjct: 257 KDIITRY----------------GQQSHHITKLDKPLQVQVEKNMPAELNKEVADRTQQL 388
Query: 127 HKETEAKFWWACVVDGMNRDQLEIFKKALEELKKLVIQ 164
F +G+N + L+ +K+LE K VI+
Sbjct: 389 RGMKSEDF------EGLNLEGLQQLEKSLESXLKRVIE 484
>TC78724 similar to GP|4322475|gb|AAD16052.1| putative MADS box
transcription factor ETL {Eucalyptus globulus subsp.
globulus}, partial (71%)
Length = 1089
Score = 79.0 bits (193), Expect = 1e-15
Identities = 43/116 (37%), Positives = 65/116 (55%)
Frame = +1
Query: 10 RQKIEMKKISNESNLQVTFSKHHNGLFKKASELCTLCGADVALVVFSPSGKVFSFGHPNL 69
R K +MK+I NESN QVTFSK NGL KKA EL LC A+VAL+VFS +GK++ F ++
Sbjct: 232 RGKTQMKRIENESNRQVTFSKRRNGLLKKAFELSVLCDAEVALIVFSTTGKLYEFSSSSI 411
Query: 70 DTVIDRFLSLIPTQNDGTMQFIEAHRNANVRELNAQLTQINNTLDAEKKIGDELSN 125
++R+ + T E ++ +++ + L K +G+EL +
Sbjct: 412 SKTVERYQGKVKELVLSTKGIQENTQHLKECDIDTTKKLEHLELSKRKLLGEELGS 579
>TC78994 similar to SP|O64645|SOC1_ARATH SUPPRESSOR OF CONSTANS
OVEREXPRESSION 1 protein (Agamous-like MADS box protein
AGL20)., partial (83%)
Length = 946
Score = 77.8 bits (190), Expect = 3e-15
Identities = 63/224 (28%), Positives = 106/224 (47%), Gaps = 12/224 (5%)
Frame = +3
Query: 5 RKGRG--RQKIEMKKISNESNLQVTFSKHHNGLFKKASELCTLCGADVALVVFSPSGKVF 62
RKG+ R K +MK+I N ++ QVTFSK NGL KKA EL LC A+VAL+VFSP G+++
Sbjct: 39 RKGKKMVRGKTQMKRIENATSRQVTFSKRRNGLLKKAFELSVLCDAEVALIVFSPRGRLY 218
Query: 63 SFGHPNLDTVIDRFLSLIPTQNDGTMQFIEAHRNANVRELNAQLTQINNTLDAEKKI--- 119
F ++ I+R+ S N T + E + +I+ +++K+
Sbjct: 219 EFASSSILETIERYRSHTRINNTPTTSESVENTQQLKEEAENMMKKIDLLETSKRKLLGE 398
Query: 120 ------GDELSNLHKETEAKFWWACVVDGMNRDQLEIFKKALEELK-KLVIQHAATRTLP 172
DEL + ++ E ++ + + ++F++ +++LK K A L
Sbjct: 399 GLGSCSIDELQKIEQQLEKS------INKIRVKKTKVFREQIDQLKEKEKALVAENVRLS 560
Query: 173 FFVGNTSSSNIYLHHQPNPQQAEMFPPQIFQNAMLQLQTHFFDG 216
GN S+ + N +AE + Q + ++T F G
Sbjct: 561 EKYGNYSTQESTKDQRENIAEAEPYADQ--SSPSSDVETELFIG 686
>BE124909 similar to GP|4322475|gb| putative MADS box transcription factor
ETL {Eucalyptus globulus subsp. globulus}, partial (60%)
Length = 608
Score = 77.8 bits (190), Expect = 3e-15
Identities = 36/67 (53%), Positives = 48/67 (70%)
Frame = +1
Query: 10 RQKIEMKKISNESNLQVTFSKHHNGLFKKASELCTLCGADVALVVFSPSGKVFSFGHPNL 69
R K +MK+I NESN QVTFSK NGL KKA EL LC A+VAL+VFS +GK++ F ++
Sbjct: 154 RGKTQMKRIENESNRQVTFSKRRNGLLKKAFELSVLCDAEVALIVFSTTGKLYEFSSSSI 333
Query: 70 DTVIDRF 76
++R+
Sbjct: 334 SKTVERY 354
>TC86876 similar to GP|16973296|emb|CAC80857. C-type MADS box protein {Malus
x domestica}, partial (85%)
Length = 2050
Score = 75.5 bits (184), Expect = 2e-14
Identities = 37/74 (50%), Positives = 51/74 (68%)
Frame = +2
Query: 3 SVRKGRGRQKIEMKKISNESNLQVTFSKHHNGLFKKASELCTLCGADVALVVFSPSGKVF 62
S +K GR KIE+K+I N +N QVTF K NGL KKA EL LC A+VALVVFS G+++
Sbjct: 74 SSQKKMGRGKIEIKRIENTTNRQVTFCKRRNGLLKKAYELSVLCDAEVALVVFSTRGRLY 253
Query: 63 SFGHPNLDTVIDRF 76
+ + ++ I+R+
Sbjct: 254 EYANNSVRATIERY 295
>TC87621 similar to SP|Q9FVC1|SVP_ARATH SHORT VEGETATIVE PHASE protein.
[Mouse-ear cress] {Arabidopsis thaliana}, partial (65%)
Length = 1322
Score = 75.1 bits (183), Expect = 2e-14
Identities = 34/71 (47%), Positives = 50/71 (69%)
Frame = +2
Query: 5 RKGRGRQKIEMKKISNESNLQVTFSKHHNGLFKKASELCTLCGADVALVVFSPSGKVFSF 64
R+ R+KI++KKI N + QVTFSK GL KKA EL LC ADVAL++FS +GK+F +
Sbjct: 428 RRDMAREKIQIKKIENSTARQVTFSKRRRGLIKKAEELSVLCDADVALIIFSSTGKLFEY 607
Query: 65 GHPNLDTVIDR 75
+ ++ +++R
Sbjct: 608 SNLSMREILER 640
>TC81292 homologue to PIR|T09700|T09700 MADS-box protein - alfalfa
(fragment), partial (91%)
Length = 800
Score = 73.9 bits (180), Expect = 5e-14
Identities = 43/119 (36%), Positives = 69/119 (57%), Gaps = 1/119 (0%)
Frame = +2
Query: 9 GRQKIEMKKISNESNLQVTFSKHHNGLFKKASELCTLCGADVALVVFSPSGKVFSFGHPN 68
GR KI++++I N ++ QVTFSK NGL KKA EL LC A+V +++FS + K++ F +
Sbjct: 74 GRGKIQIRRIDNSTSRQVTFSKRRNGLLKKAKELAILCDAEVGVMIFSSTAKLYDFASTS 253
Query: 69 LDTVIDRFLSLIPTQNDGTMQFIEAHRNANVRELNAQLTQINNTLDAEKKI-GDELSNL 126
+ +VIDR+ N + + RE Q++N ++ ++I G+ELS L
Sbjct: 254 MRSVIDRYNKTKEEHNQ--LGSSTSEIKFWQREAAMLRQQLHNLQESHRQIMGEELSGL 424
>TC82227 similar to GP|1483232|emb|CAA67969.1 MADS5 protein {Betula
pendula}, partial (68%)
Length = 681
Score = 71.2 bits (173), Expect = 3e-13
Identities = 31/69 (44%), Positives = 50/69 (71%), Gaps = 1/69 (1%)
Frame = +1
Query: 9 GRQKIEMKKISNESNLQVTFSKHHNGLFKKASELCTLCGADVALVVFSPSGKVFSF-GHP 67
GR ++++K+I N+ N QVTFSK +GL KKA E+ LC A+VAL++FS GK+F + P
Sbjct: 133 GRGRVQLKRIENKINRQVTFSKRRSGLLKKAQEISVLCDAEVALIIFSTKGKLFEYSSDP 312
Query: 68 NLDTVIDRF 76
++ +++R+
Sbjct: 313 CMEKILERY 339
>TC84496 similar to GP|1483232|emb|CAA67969.1 MADS5 protein {Betula
pendula}, partial (74%)
Length = 681
Score = 70.9 bits (172), Expect = 4e-13
Identities = 32/69 (46%), Positives = 50/69 (72%), Gaps = 1/69 (1%)
Frame = +2
Query: 9 GRQKIEMKKISNESNLQVTFSKHHNGLFKKASELCTLCGADVALVVFSPSGKVFSF-GHP 67
GR ++++KKI N+ N QVTFSK +GL KKA E+ LC A+VAL+VFS GK++ + P
Sbjct: 29 GRGRVQLKKIENKINRQVTFSKRRSGLLKKAHEISVLCDAEVALIVFSNKGKLYEYSSDP 208
Query: 68 NLDTVIDRF 76
++ +++R+
Sbjct: 209CMEKILERY 235
>AL387855 similar to GP|1483230|emb MADS4 protein {Betula pendula}, partial
(51%)
Length = 493
Score = 70.5 bits (171), Expect = 5e-13
Identities = 37/119 (31%), Positives = 69/119 (57%), Gaps = 1/119 (0%)
Frame = +2
Query: 9 GRQKIEMKKISNESNLQVTFSKHHNGLFKKASELCTLCGADVALVVFSPSGKVFSFGH-P 67
GR ++++K+I N+++ QVTF K GL KKA+E+ LC A VAL++FS GK+F + P
Sbjct: 56 GRGRVQLKRIENKTSQQVTFFKRRTGLLKKANEISVLCDAQVALIMFSTKGKLFEYSSAP 235
Query: 68 NLDTVIDRFLSLIPTQNDGTMQFIEAHRNANVRELNAQLTQINNTLDAEKKIGDELSNL 126
+++ +++R+ T+ G + + + +L A++ + L +G++L L
Sbjct: 236 SMEDILERYERQNHTELTGATNETQGNWSFEYMKLTAKVQVLERNL--RNFVGNDLDPL 406
>TC93057 homologue to GP|23194453|gb|AAN15183.1 MADS box protein GHMADS-2
{Gossypium hirsutum}, partial (95%)
Length = 858
Score = 70.1 bits (170), Expect = 7e-13
Identities = 45/124 (36%), Positives = 66/124 (52%), Gaps = 6/124 (4%)
Frame = +3
Query: 9 GRQKIEMKKISNESNLQVTFSKHHNGLFKKASELCTLCGADVALVVFSPSGKVFSFGHPN 68
G +++ K I N +N QVTF K NGL KKA EL LC A+VAL+VFS G+++ + + N
Sbjct: 33 GGERLR*KGIENTTNRQVTFCKRRNGLLKKAYELSVLCDAEVALIVFSSRGRLYEYSNNN 212
Query: 69 LDTVIDRFL------SLIPTQNDGTMQFIEAHRNANVRELNAQLTQINNTLDAEKKIGDE 122
+ + IDR+ S T + Q+ + +A +R+ L N L +GD
Sbjct: 213 IRSTIDRYKKACSDHSSTTTTTEINAQYYQ-QESAKLRQQIQMLQNSNRHL-----MGDA 374
Query: 123 LSNL 126
LS L
Sbjct: 375 LSTL 386
>TC90653 similar to GP|10835358|gb|AAC13695.2 PTD protein {Populus
balsamifera subsp. trichocarpa}, partial (59%)
Length = 845
Score = 70.1 bits (170), Expect = 7e-13
Identities = 44/113 (38%), Positives = 65/113 (56%), Gaps = 2/113 (1%)
Frame = +2
Query: 3 SVRKGRGRQKIEMKKISNESNLQVTFSKHHNGLFKKASELCTLCGADVALVVFSPSGKVF 62
S++ GRG KIE+K I N +N QVT+SK NG+FKKA EL LC A V+L++FS + K+
Sbjct: 35 SLKMGRG--KIEIKLIENPTNRQVTYSKRRNGIFKKAHELSVLCDAKVSLIMFSKNNKMH 208
Query: 63 SFGHPNLDT--VIDRFLSLIPTQNDGTMQFIEAHRNANVRELNAQLTQINNTL 113
+ P L T +ID++ + G + +H + L +L IN+ L
Sbjct: 209 EYITPGLSTKKIIDQYQKTL-----GDIDLWRSHYEKMLENLK-KLKDINHKL 349
>TC82622 similar to GP|21739238|emb|CAD40993. OSJNBa0072F16.17 {Oryza
sativa}, partial (78%)
Length = 847
Score = 69.3 bits (168), Expect = 1e-12
Identities = 32/68 (47%), Positives = 48/68 (70%)
Frame = +3
Query: 9 GRQKIEMKKISNESNLQVTFSKHHNGLFKKASELCTLCGADVALVVFSPSGKVFSFGHPN 68
GR KI +++I N ++ QVTFSK GL KKA EL LC A V LV+FS +GK++ + + +
Sbjct: 141 GRGKIVIRRIDNCTSRQVTFSKRRKGLIKKAKELAILCDAQVGLVIFSSTGKLYEYANTS 320
Query: 69 LDTVIDRF 76
+ +VI+R+
Sbjct: 321 MKSVIERY 344
>TC84438 homologue to GP|1483232|emb|CAA67969.1 MADS5 protein {Betula
pendula}, partial (24%)
Length = 702
Score = 67.8 bits (164), Expect = 3e-12
Identities = 29/56 (51%), Positives = 42/56 (74%)
Frame = +1
Query: 9 GRQKIEMKKISNESNLQVTFSKHHNGLFKKASELCTLCGADVALVVFSPSGKVFSF 64
GR ++++K+I N+ N QVTFSK +GL KKA E+ LC A+VAL++FS GK+F +
Sbjct: 226 GRGRVQLKRIENKINRQVTFSKRRSGLLKKAQEISVLCDAEVALIIFSTKGKLFEY 393
>AW688164 similar to GP|3114588|gb|A MADS box protein {Eucalyptus grandis},
partial (31%)
Length = 549
Score = 49.3 bits (116), Expect(2) = 2e-08
Identities = 22/40 (55%), Positives = 29/40 (72%)
Frame = +1
Query: 9 GRQKIEMKKISNESNLQVTFSKHHNGLFKKASELCTLCGA 48
GR ++ +++I N+ N QVTFSK +GL KKA ELC LC A
Sbjct: 10 GRGRVVLERIENKINRQVTFSKRRSGLLKKAFELCVLCDA 129
Score = 25.8 bits (55), Expect(2) = 2e-08
Identities = 10/29 (34%), Positives = 21/29 (71%), Gaps = 1/29 (3%)
Frame = +2
Query: 49 DVALVVFSPSGKVFSFGH-PNLDTVIDRF 76
+VAL++FS GK+F + +++ +I+R+
Sbjct: 131 EVALIIFSSRGKLFQYSSTTDINKIIERY 217
>BI272814 PIR|S71757|S71 MADS box protein DEFH200 - garden snapdragon,
partial (16%)
Length = 320
Score = 49.3 bits (116), Expect = 1e-06
Identities = 22/38 (57%), Positives = 28/38 (72%)
Frame = +1
Query: 9 GRQKIEMKKISNESNLQVTFSKHHNGLFKKASELCTLC 46
GR ++E+K+I N+ N QVTF+K NGL KKA EL LC
Sbjct: 202 GRGRVELKRIENKINRQVTFAKRRNGLLKKAYELSVLC 315
>BE999756 weakly similar to GP|9758603|dbj| gene_id:MDF20.13~unknown protein
{Arabidopsis thaliana}, partial (15%)
Length = 534
Score = 39.7 bits (91), Expect = 0.001
Identities = 26/98 (26%), Positives = 48/98 (48%), Gaps = 3/98 (3%)
Frame = +1
Query: 12 KIEMKKISNESNLQVTFSKHHNGLFKKASELCTLCGADVALVVFSPSGKVFSFGHPNLD- 70
K+++ I+N + + T+ K L KK +E+ TLCG +VF P P+++
Sbjct: 25 KVKLAFITNHTARRATYKKRVQSLMKKLNEITTLCGVKACGIVFKPDDLEPQI-WPSIEG 201
Query: 71 --TVIDRFLSLIPTQNDGTMQFIEAHRNANVRELNAQL 106
+V+ RF+ D M E++ +++LN +L
Sbjct: 202 VHSVLVRFMQTPNFYRDRKMFDHESYLKERIQKLNEKL 315
>TC91615 weakly similar to GP|21593574|gb|AAM65541.1 unknown {Arabidopsis
thaliana}, partial (50%)
Length = 834
Score = 31.6 bits (70), Expect = 0.26
Identities = 29/87 (33%), Positives = 44/87 (50%), Gaps = 1/87 (1%)
Frame = +2
Query: 44 TLCGADVALVVF-SPSGKVFSFGHPNLDTVIDRFLSLIPTQNDGTMQFIEAHRNANVREL 102
T C VA+ +F P+ +F N T+ FLSL+PTQN ++A+R VR L
Sbjct: 116 TCCPFQVAIAIFLKPTHTIFL----NKKTLTRFFLSLLPTQN------MDANRRV-VRTL 262
Query: 103 NAQLTQINNTLDAEKKIGDELSNLHKE 129
++L+ ++ E I EL L K+
Sbjct: 263 VSKLSSVSEKTRIESLI--ELRQLSKQ 337
>TC89605
Length = 1041
Score = 31.2 bits (69), Expect = 0.34
Identities = 46/201 (22%), Positives = 78/201 (37%), Gaps = 7/201 (3%)
Frame = +1
Query: 2 SSVRKGRGRQKIEMKKISNESNLQVTFSKHHNGLFKKASELCTLCGADVALVVFSPSGKV 61
S + RG I + E + +++ +KK S L ++ DV LV FS +
Sbjct: 415 SDINLERGEDDIVQNTVLIEISKLCADARNIAFYYKKKSGLISM--KDVGLVDFSIPSE- 585
Query: 62 FSFGHPNLDTVIDRFLSLIPTQNDGTMQFIEAHRNANVRELNAQLTQINN-------TLD 114
L+ I LSL PT + QF AN++EL +L + + T
Sbjct: 586 ------GLNICIKLLLSL-PTDKESNTQFDVLEAEANIKELKLRLHETRHDFLYTFLTPL 744
Query: 115 AEKKIGDELSNLHKETEAKFWWACVVDGMNRDQLEIFKKALEELKKLVIQHAATRTLPFF 174
EK++ +L +++ R LE K+ L +L++ V +
Sbjct: 745 VEKRLKRQLEE-------------IMNNYIRKSLEYLKENLAKLRQKVDE---------- 855
Query: 175 VGNTSSSNIYLHHQPNPQQAE 195
+ S N + HQ + Q+ E
Sbjct: 856 MQTDSQENPLISHQKHEQRKE 918
>TC92960 weakly similar to GP|10177030|dbj|BAB10268. contains similarity to
protein kinase~gene_id:MQJ2.14 {Arabidopsis thaliana},
partial (21%)
Length = 841
Score = 28.9 bits (63), Expect = 1.7
Identities = 11/35 (31%), Positives = 18/35 (51%)
Frame = +1
Query: 181 SNIYLHHQPNPQQAEMFPPQIFQNAMLQLQTHFFD 215
+N H+QP P + PP I ++ + +HF D
Sbjct: 100 NNHRFHNQPPPPPSSSSPPHILPKSLTHIHSHFQD 204
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.320 0.133 0.393
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 7,184,407
Number of Sequences: 36976
Number of extensions: 100564
Number of successful extensions: 661
Number of sequences better than 10.0: 47
Number of HSP's better than 10.0 without gapping: 658
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 661
length of query: 228
length of database: 9,014,727
effective HSP length: 93
effective length of query: 135
effective length of database: 5,575,959
effective search space: 752754465
effective search space used: 752754465
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 56 (26.2 bits)
Medicago: description of AC144731.7