BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC144728.5 - phase: 0
(497 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC93239 similar to GP|16612271|gb|AAL27503.1 At2g47010/F14M4.16 ... 338 2e-93
TC85265 similar to GP|10177300|dbj|BAB10561. topoisomerase-like ... 30 2.1
TC90492 similar to PIR|C86308|C86308 hypothetical protein AAD500... 30 2.1
BG585334 similar to GP|20161490|db beta-1 3 glucanase-like prote... 29 3.6
TC91688 29 4.8
TC85745 homologue to GP|17297987|dbj|BAB78491. 26S proteasome re... 28 6.2
BE324707 similar to PIR|F96765|F967 unknown protein F25P22.24 [i... 28 8.1
TC83262 similar to PIR|F96765|F96765 unknown protein F25P22.24 [... 28 8.1
TC84419 similar to SP|Q42920|PME_MEDSA Pectinesterase precursor ... 28 8.1
>TC93239 similar to GP|16612271|gb|AAL27503.1 At2g47010/F14M4.16
{Arabidopsis thaliana}, partial (20%)
Length = 659
Score = 338 bits (868), Expect = 2e-93
Identities = 169/181 (93%), Positives = 169/181 (93%), Gaps = 2/181 (1%)
Frame = +1
Query: 1 MEKQLQKFFVLSNVMLLCASLVSTTQSQTNVSAVGDPGMQRDGLRVAFEAWNFCNEVGQE 60
MEKQLQKFFVLSNVMLLCASLVSTTQSQTNVSAVGDPGMQRDGLRVAFEAWNFCNEVGQE
Sbjct: 52 MEKQLQKFFVLSNVMLLCASLVSTTQSQTNVSAVGDPGMQRDGLRVAFEAWNFCNEVGQE 231
Query: 61 APYMGSPRAAQCFDLSQGSLIHKVTEKDNKLGVGESLPGLRPEDINNIDLYAVQKELYLG 120
APYMGSPRAAQCFDLSQGSLIHKVTEKDNKLGVGESLPGLRPEDINNIDLYAVQKELYLG
Sbjct: 232 APYMGSPRAAQCFDLSQGSLIHKVTEKDNKLGVGESLPGLRPEDINNIDLYAVQKELYLG 411
Query: 121 SLCEVKDTPRPWQFWMIMLKNGNYDSRSGLCPQDGKRVPPFKPGRFPC--FGTGCMNQPI 178
SLCEVKDTPRPWQFWMIMLKNGNYDSRSGLCPQDGKRVP F R C FGTGCM
Sbjct: 412 SLCEVKDTPRPWQFWMIMLKNGNYDSRSGLCPQDGKRVPAF*T-RKVCLVFGTGCMKPTY 588
Query: 179 L 179
L
Sbjct: 589 L 591
Score = 54.7 bits (130), Expect = 8e-08
Identities = 25/36 (69%), Positives = 26/36 (71%), Gaps = 1/36 (2%)
Frame = +3
Query: 167 PCFGTGCMNQPILC-HQWTKLEGDVMRGGFNGTYDL 201
PCF + C HQWTKLEGDVMRGGFNGTYDL
Sbjct: 552 PCFWNRMHETNLSCVHQWTKLEGDVMRGGFNGTYDL 659
Score = 28.5 bits (62), Expect = 6.2
Identities = 15/27 (55%), Positives = 15/27 (55%), Gaps = 1/27 (3%)
Frame = +2
Query: 160 PFKPGRFPCF-GTGCMNQPILCHQWTK 185
PFKPGRF F NQPILC K
Sbjct: 530 PFKPGRFALFLEQDA*NQPILCASMDK 610
>TC85265 similar to GP|10177300|dbj|BAB10561. topoisomerase-like protein
{Arabidopsis thaliana}, partial (56%)
Length = 1803
Score = 30.0 bits (66), Expect = 2.1
Identities = 16/64 (25%), Positives = 28/64 (43%), Gaps = 2/64 (3%)
Frame = -1
Query: 349 FHITPDNKKIFRNDTANFPYSAYHYYC--APGNAQHLEQPVSTCDPYSNPQAQEIVQLLP 406
FH+ + K++F PY+ +HY+C Q + P S P+ + L
Sbjct: 897 FHLHDEQKRVFE------PYAQFHYHCILVVVCCQFRPSSMGQVHPQSQPELKTEQAHLK 736
Query: 407 HPIW 410
HP++
Sbjct: 735 HPVY 724
>TC90492 similar to PIR|C86308|C86308 hypothetical protein AAD50012.1
[imported] - Arabidopsis thaliana, partial (19%)
Length = 1338
Score = 30.0 bits (66), Expect = 2.1
Identities = 22/90 (24%), Positives = 37/90 (40%), Gaps = 6/90 (6%)
Frame = +2
Query: 23 STTQSQTNVSAVGDPGMQRDGLRVAFEAWNFCNEVGQEAPYMGSPRAAQCFDLSQGSLIH 82
ST + N +A RDG + + EA G+ + + G + H
Sbjct: 830 STNYGEENFNAETVTAQARDGFSLGISGGSVGMCASHEAEIHGADVSVHRTNSVVGDMEH 1009
Query: 83 KVTEKDNKLGVGESLPG------LRPEDIN 106
+V + +N+ GES+P + P+DIN
Sbjct: 1010RVEDAENQGQTGESVPDPGLMDEIIPDDIN 1099
>BG585334 similar to GP|20161490|db beta-1 3 glucanase-like protein {Oryza
sativa (japonica cultivar-group)}, partial (39%)
Length = 835
Score = 29.3 bits (64), Expect = 3.6
Identities = 21/68 (30%), Positives = 29/68 (41%), Gaps = 1/68 (1%)
Frame = +1
Query: 330 INPETPAWCSPTGLGNCPPFHITPDNKKIF-RNDTANFPYSAYHYYCAPGNAQHLEQPVS 388
INP +P + G PP +PD N +N P S Y P + P +
Sbjct: 196 INPTSPQPPDTSTGGQNPP---SPDTDTTSPTNPYSNPPTSTSPYSNPPASTNPYSNPPA 366
Query: 389 TCDPYSNP 396
+ +PYSNP
Sbjct: 367 STNPYSNP 390
>TC91688
Length = 552
Score = 28.9 bits (63), Expect = 4.8
Identities = 16/54 (29%), Positives = 24/54 (43%)
Frame = -3
Query: 296 SQFYLLDMGSCWKNNGAPCDGDVLTDVTRYSEMIINPETPAWCSPTGLGNCPPF 349
S F +L + + GA + V + +IINPE P C+P C P+
Sbjct: 517 SSFTILGINK*KEYRGARMKNQIFQQVAAHPYVIINPEVPFNCAPY-WSRCEPY 359
>TC85745 homologue to GP|17297987|dbj|BAB78491. 26S proteasome regulatory
particle triple-A ATPase subunit2b {Oryza sativa
(japonica cultivar-group), partial (98%)
Length = 1696
Score = 28.5 bits (62), Expect = 6.2
Identities = 20/64 (31%), Positives = 34/64 (52%), Gaps = 4/64 (6%)
Frame = -1
Query: 255 TVSGFSG-GYHYDTRGMLKAPLESPN--FKVRLTLDVKKGGGSKSQFY-LLDMGSCWKNN 310
T+S F+ H+DT G+ E+ + F +R + GG + S F+ L + SC+++
Sbjct: 301 TLSSFNSLSLHFDT-GVTVGNREAASGPFCLRCLRPTRAGGAAGSNFFSFLSLPSCFRSP 125
Query: 311 GAPC 314
G PC
Sbjct: 124 GWPC 113
>BE324707 similar to PIR|F96765|F967 unknown protein F25P22.24 [imported] -
Arabidopsis thaliana, partial (37%)
Length = 530
Score = 28.1 bits (61), Expect = 8.1
Identities = 11/22 (50%), Positives = 14/22 (63%)
Frame = -1
Query: 421 DGWVGDARTWELDVGGLASRLY 442
D W + RTWEL+VG + R Y
Sbjct: 248 DIWFSEGRTWELNVGTCSIRRY 183
>TC83262 similar to PIR|F96765|F96765 unknown protein F25P22.24 [imported] -
Arabidopsis thaliana, complete
Length = 716
Score = 28.1 bits (61), Expect = 8.1
Identities = 11/22 (50%), Positives = 14/22 (63%)
Frame = -3
Query: 421 DGWVGDARTWELDVGGLASRLY 442
D W + RTWEL+VG + R Y
Sbjct: 252 DIWFSEGRTWELNVGTCSIRRY 187
>TC84419 similar to SP|Q42920|PME_MEDSA Pectinesterase precursor (EC
3.1.1.11) (Pectin methylesterase) (PE) (P65). [Alfalfa],
partial (53%)
Length = 732
Score = 28.1 bits (61), Expect = 8.1
Identities = 13/44 (29%), Positives = 20/44 (44%), Gaps = 2/44 (4%)
Frame = -1
Query: 94 GESLPGLRPEDINNID--LYAVQKELYLGSLCEVKDTPRPWQFW 135
G P +R I ++ L+ + K LG + +P WQFW
Sbjct: 534 GTKRPSMRESTITTLEKFLHGLPK*AILGCMVRTSGSPVKWQFW 403
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.318 0.138 0.446
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 18,620,591
Number of Sequences: 36976
Number of extensions: 302667
Number of successful extensions: 1259
Number of sequences better than 10.0: 18
Number of HSP's better than 10.0 without gapping: 1251
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1259
length of query: 497
length of database: 9,014,727
effective HSP length: 100
effective length of query: 397
effective length of database: 5,317,127
effective search space: 2110899419
effective search space used: 2110899419
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 60 (27.7 bits)
Medicago: description of AC144728.5