BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC144728.11 + phase: 0 /pseudo
(438 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BG587113 weakly similar to PIR|A84888|A8 hypothetical protein At... 46 2e-05
TC83226 weakly similar to PIR|G86419|G86419 probable reverse tra... 42 6e-04
TC93101 40 0.002
BQ149324 35 0.003
TC82948 35 0.057
BE124667 33 0.17
BG585866 33 0.17
BG456581 33 0.28
AW980456 32 0.63
TC91765 weakly similar to GP|19881779|gb|AAM01180.1 Putative ret... 31 1.1
BF650593 30 1.8
TC85631 homologue to SP|Q9BBN9|NU1C_LOTJA NADH-plastoquinone oxi... 29 3.1
BG587173 weakly similar to GP|4512630|gb| Contains reverse trans... 29 4.1
TC93881 similar to GP|9081779|dbj|BAA99518.1 hypothetical protei... 28 5.3
TC79517 similar to PIR|T08874|T08874 calcium-dependent protein k... 28 5.3
TC91586 similar to GP|22655178|gb|AAM98179.1 Ca2+-dependent lipi... 28 7.0
BG457088 similar to GP|23504661|emb hypothetical protein conser... 28 7.0
TC82878 similar to PIR|T45677|T45677 ATP-dependent RNA helicase-... 28 9.1
TC78658 similar to PIR|A96683|A96683 hypothetical protein F12P19... 28 9.1
TC81629 similar to GP|15081654|gb|AAK82482.1 AT4g26750/F10M23_90... 28 9.1
>BG587113 weakly similar to PIR|A84888|A8 hypothetical protein At2g45230
[imported] - Arabidopsis thaliana, partial (10%)
Length = 767
Score = 46.2 bits (108), Expect = 2e-05
Identities = 15/50 (30%), Positives = 31/50 (62%)
Frame = -3
Query: 316 CVGGCLPTRVLLQDKGVSCPTNCASCSHEYEDLNHLMFECPFSIQVWMSA 365
C+ LPT ++ + +S +C+ C E E +NH++F+CP++ +W ++
Sbjct: 240 CISNSLPTAANMRSRHISKDGSCSRCGMESETVNHILFQCPYARLIWATS 91
>TC83226 weakly similar to PIR|G86419|G86419 probable reverse transcriptase
100033-105622 [imported] - Arabidopsis thaliana, partial
(2%)
Length = 885
Score = 41.6 bits (96), Expect = 6e-04
Identities = 16/47 (34%), Positives = 24/47 (51%)
Frame = +3
Query: 321 LPTRVLLQDKGVSCPTNCASCSHEYEDLNHLMFECPFSIQVWMSAGM 367
LP R L+ +G+ C C C + E + HL CP S +VW + +
Sbjct: 192 LPVRSSLRKRGIQCYPLCPRCHSKTETITHLFMSCPLSKRVWFGSNL 332
>TC93101
Length = 675
Score = 39.7 bits (91), Expect = 0.002
Identities = 16/45 (35%), Positives = 22/45 (48%)
Frame = -1
Query: 321 LPTRVLLQDKGVSCPTNCASCSHEYEDLNHLMFECPFSIQVWMSA 365
LP R L +GV+CP C C E NH+ C + +VW +
Sbjct: 663 LPVRXELNKRGVNCPPLCPRCYFNLETTNHIFMSCERTQRVWFGS 529
>BQ149324
Length = 374
Score = 35.0 bits (79), Expect(2) = 0.003
Identities = 15/27 (55%), Positives = 19/27 (69%)
Frame = +3
Query: 321 LPTRVLLQDKGVSCPTNCASCSHEYED 347
LPTRV L+DK V+CP +C C+ ED
Sbjct: 3 LPTRVRLKDKRVTCPMDCTLCTVGSED 83
Score = 23.1 bits (48), Expect(2) = 0.003
Identities = 7/13 (53%), Positives = 10/13 (76%)
Frame = +1
Query: 350 HLMFECPFSIQVW 362
HL+F+C S+ VW
Sbjct: 91 HLIFQCSSSLNVW 129
>TC82948
Length = 705
Score = 35.0 bits (79), Expect = 0.057
Identities = 17/57 (29%), Positives = 26/57 (44%)
Frame = +3
Query: 338 CASCSHEYEDLNHLMFECPFSIQVWMSAGMWYMCKMRP*IRTLLSTQFSIYSKTRLW 394
C+ C +E +HL FEC S+Q+W W+ L++ F I + LW
Sbjct: 444 CSLCFKSFESTHHLFFECSVSVQIW----DWF--------SNLINLNFHINNTDDLW 578
>BE124667
Length = 540
Score = 33.5 bits (75), Expect = 0.17
Identities = 17/60 (28%), Positives = 27/60 (44%)
Frame = -1
Query: 335 PTNCASCSHEYEDLNHLMFECPFSIQVWMSAGMWYMCKMRP*IRTLLSTQFSIYSKTRLW 394
P+ C+SC E HL FEC F+ ++W W + +LL+T + +W
Sbjct: 393 PSMCSSCQASSETTFHLFFECNFATKMW----SW--------LASLLNTPLQFSTSADMW 250
>BG585866
Length = 828
Score = 33.5 bits (75), Expect = 0.17
Identities = 20/86 (23%), Positives = 33/86 (38%), Gaps = 3/86 (3%)
Frame = +3
Query: 284 SWLMFPTSIVRVIGETFG---ALRSPRKLNT*FGVCVGGCLPTRVLLQDKGVSCPTNCAS 340
SW++ T V ++ L+ P K + +PT LL + + C+
Sbjct: 405 SWILSQTETVNYNNSSWSWIWRLKIPEKYKFFLWLACHNAVPTLSLLNHRNMVNSAICSR 584
Query: 341 CSHEYEDLNHLMFECPFSIQVWMSAG 366
C E H + +C FS +W G
Sbjct: 585 CGEHEESFFHCVRDCRFSKIIWHKIG 662
>BG456581
Length = 683
Score = 32.7 bits (73), Expect = 0.28
Identities = 13/42 (30%), Positives = 21/42 (49%)
Frame = +2
Query: 321 LPTRVLLQDKGVSCPTNCASCSHEYEDLNHLMFECPFSIQVW 362
LPT L +G + + C+ C + E +HL C F + +W
Sbjct: 143 LPTDDNLSSRGCALVSMCSFCLEQVETSDHLFLRCKFVVTLW 268
>AW980456
Length = 779
Score = 31.6 bits (70), Expect = 0.63
Identities = 21/56 (37%), Positives = 29/56 (51%)
Frame = -3
Query: 263 FGKWRGTIFIRYVALTVFVLKSWLMFPTSIVRVIGETFGALRSPRKLNT*FGVCVG 318
FG+ + I L VFV ++ + T V VIG FG+L+ + T* G CVG
Sbjct: 198 FGRMKSMGNIM*RVLIVFVSRNSSILLTFTVPVIGPEFGSLKYLLRFKT*SGGCVG 31
>TC91765 weakly similar to GP|19881779|gb|AAM01180.1 Putative retroelement
{Oryza sativa (japonica cultivar-group)}, partial (1%)
Length = 625
Score = 30.8 bits (68), Expect = 1.1
Identities = 14/21 (66%), Positives = 16/21 (75%)
Frame = +1
Query: 12 RP*SVVIEQPLLLILRIVCGK 32
RP*S IEQ L L+LR+ CGK
Sbjct: 505 RP*SAEIEQQLFLLLRVECGK 567
>BF650593
Length = 486
Score = 30.0 bits (66), Expect = 1.8
Identities = 15/42 (35%), Positives = 20/42 (46%)
Frame = +3
Query: 321 LPTRVLLQDKGVSCPTNCASCSHEYEDLNHLMFECPFSIQVW 362
L R +L + C +C E ++H FECPFS VW
Sbjct: 372 LSKRGVLDQNSIXCVXDCG----REESVSHFFFECPFS-XVW 482
>TC85631 homologue to SP|Q9BBN9|NU1C_LOTJA NADH-plastoquinone oxidoreductase
chain 1 chloroplast (EC 1.6.5.3). {Lotus japonicus},
complete
Length = 3706
Score = 29.3 bits (64), Expect = 3.1
Identities = 18/48 (37%), Positives = 23/48 (47%), Gaps = 4/48 (8%)
Frame = +1
Query: 303 LRSPRKLN--T*FGVCV--GGCLPTRVLLQDKGVSCPTNCASCSHEYE 346
+R R LN FG+C+ G C+ CPTNC S + EYE
Sbjct: 3418 IRKKRLLNYSIDFGICIFCGNCIEY----------CPTNCLSMTEEYE 3531
>BG587173 weakly similar to GP|4512630|gb| Contains reverse transcriptase
domain (rvt) PF|00078. {Arabidopsis thaliana}, partial
(5%)
Length = 292
Score = 28.9 bits (63), Expect = 4.1
Identities = 14/38 (36%), Positives = 15/38 (38%)
Frame = +1
Query: 321 LPTRVLLQDKGVSCPTNCASCSHEYEDLNHLMFECPFS 358
LPTR S C C H E +HL C FS
Sbjct: 163 LPTRCRFAAWDTSIVKTCLLCGHYDESRDHLFLRCSFS 276
>TC93881 similar to GP|9081779|dbj|BAA99518.1 hypothetical protein {Oryza
sativa (japonica cultivar-group)}, partial (27%)
Length = 826
Score = 28.5 bits (62), Expect = 5.3
Identities = 18/57 (31%), Positives = 30/57 (52%)
Frame = -2
Query: 132 FLLILNHLLLAFLRLDTF*IIPISLRLSVTIRVTCGVAFCVPDLLCVVGLVGVLVLV 188
F +IL LLL F+ +F + S S++ ++C V F V + V L+G ++V
Sbjct: 510 FGIILPALLLPFVFFFSFLPLTASTAASISASISCAVGFSVLEYFTVPILLGSSMIV 340
>TC79517 similar to PIR|T08874|T08874 calcium-dependent protein kinase (EC
2.7.1.-) gamma - soybean, partial (80%)
Length = 1930
Score = 28.5 bits (62), Expect = 5.3
Identities = 17/54 (31%), Positives = 24/54 (43%), Gaps = 6/54 (11%)
Frame = +2
Query: 296 IGETFGALRSPRKLNT*FGVCVGGCLPTRVLLQDK------GVSCPTNCASCSH 343
IGE GA ++ +C GG L R++ + K C TNC CS+
Sbjct: 815 IGEFKGAYEDKNSVHVVMELCAGGELFDRIIAKGKYSERAAASICKTNC*CCSY 976
>TC91586 similar to GP|22655178|gb|AAM98179.1 Ca2+-dependent lipid-binding
protein putative {Arabidopsis thaliana}, partial (40%)
Length = 676
Score = 28.1 bits (61), Expect = 7.0
Identities = 15/40 (37%), Positives = 22/40 (54%)
Frame = -3
Query: 303 LRSPRKLNT*FGVCVGGCLPTRVLLQDKGVSCPTNCASCS 342
L++P +N F C G PTRVL+ + P++C S S
Sbjct: 395 LKTP--VNPTFLTCTGSARPTRVLISSIILGLPSHCTSNS 282
>BG457088 similar to GP|23504661|emb hypothetical protein conserved
{Plasmodium falciparum 3D7}, partial (2%)
Length = 673
Score = 28.1 bits (61), Expect = 7.0
Identities = 13/26 (50%), Positives = 17/26 (65%)
Frame = +1
Query: 337 NCASCSHEYEDLNHLMFECPFSIQVW 362
NCASC H Y++ L+F P+ I VW
Sbjct: 337 NCASCYH-YKNNVFLVFSPPYFILVW 411
>TC82878 similar to PIR|T45677|T45677 ATP-dependent RNA helicase-like
protein - Arabidopsis thaliana, partial (33%)
Length = 1185
Score = 27.7 bits (60), Expect = 9.1
Identities = 19/46 (41%), Positives = 22/46 (47%), Gaps = 8/46 (17%)
Frame = +3
Query: 233 CLLLGKCLATTLLKPF*IHPCMN---RFRMIVLF-----GKWRGTI 270
CL LG CLA +LKP +H R M VL G WRG +
Sbjct: 816 CLQLGSCLARYMLKPVSLHIKQE*RLRLFMGVLLLDNS*GFWRGVL 953
>TC78658 similar to PIR|A96683|A96683 hypothetical protein F12P19.5
[imported] - Arabidopsis thaliana, partial (29%)
Length = 813
Score = 27.7 bits (60), Expect = 9.1
Identities = 14/37 (37%), Positives = 24/37 (64%)
Frame = +3
Query: 200 LTVSLLMVI*REASIFVILKLTVY*LIMVRFGMCLLL 236
L + L M + +E I V+LKL ++ ++++RF LLL
Sbjct: 201 LKIGLKM*LFQEVKILVVLKLKMFFIVILRF*KLLLL 311
>TC81629 similar to GP|15081654|gb|AAK82482.1 AT4g26750/F10M23_90
{Arabidopsis thaliana}, partial (19%)
Length = 824
Score = 27.7 bits (60), Expect = 9.1
Identities = 17/74 (22%), Positives = 39/74 (51%)
Frame = -2
Query: 214 IFVILKLTVY*LIMVRFGMCLLLGKCLATTLLKPF*IHPCMNRFRMIVLFGKWRGTIFIR 273
+ V++ L + + R+ + ++LG+ +L+ + + R+R I+L G+WR + +
Sbjct: 229 LLVVVGLGIRTV*SCRWRLMVILGRWRGLIILRRWRRMVILGRWRRIIL-GRWRRMVILG 53
Query: 274 YVALTVFVLKSWLM 287
VF+ + W+M
Sbjct: 52 RWRRMVFLGRWWVM 11
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.359 0.163 0.562
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 15,560,895
Number of Sequences: 36976
Number of extensions: 259279
Number of successful extensions: 2730
Number of sequences better than 10.0: 40
Number of HSP's better than 10.0 without gapping: 1517
Number of HSP's successfully gapped in prelim test: 111
Number of HSP's that attempted gapping in prelim test: 1184
Number of HSP's gapped (non-prelim): 1678
length of query: 438
length of database: 9,014,727
effective HSP length: 99
effective length of query: 339
effective length of database: 5,354,103
effective search space: 1815040917
effective search space used: 1815040917
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 14 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 37 (21.8 bits)
S2: 60 (27.7 bits)
Medicago: description of AC144728.11