Medicago
BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= AC144727.5 + phase: 0 
         (256 letters)

Database: MTGI 
           36,976 sequences; 27,044,181 total letters

Searching..................................................done


                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

BG587170 similar to PIR|F86470|F8 probable retroelement polyprot...    80  1e-15
TC93066 weakly similar to GP|19920130|gb|AAM08562.1 Putative ret...    64  4e-11
AJ499215 weakly similar to GP|6642775|gb| gag-pol polyprotein {V...    50  1e-06
AW773859                                                               48  4e-06
BQ154516                                                               36  0.017
AW736233 GP|21105451|gb small nuclear ribonucleoprotein D1 {Dani...    30  0.70
TC89711 weakly similar to PIR|T10725|T10725 protein kinase Xa21 ...    30  1.2
TC84334 weakly similar to PIR|T49308|T49308 hypothetical protein...    29  2.0
CB893545 similar to GP|8778537|gb| F22G5.5 {Arabidopsis thaliana...    27  5.9
TC82987 similar to GP|15991740|gb|AAL13047.1 WRKY transcription ...    27  7.7
BQ150771 GP|4028979|emb glutamate permease {synthetic construct}...    27  7.7
TC92435 similar to GP|18377843|gb|AAL67108.1 AT5g42350/MDH9_4 {A...    27  7.7

>BG587170 similar to PIR|F86470|F8 probable retroelement polyprotein
           [imported] - Arabidopsis thaliana, partial (13%)
          Length = 718

 Score = 79.7 bits (195), Expect = 1e-15
 Identities = 49/154 (31%), Positives = 77/154 (49%), Gaps = 4/154 (2%)
 Frame = -3

Query: 103 SGKVIAKGPKVGRLFPLQF---ISNH-LSLPCNNVLNSYEDWHRKLGHPNSTVLSHLFKT 158
           S ++I +G   G L+ L+    +SN+  S   ++ LN    WH +LGHP+   L+ +   
Sbjct: 704 SSQLIGEGVTKGDLYMLEKLDPVSNYKCSFTSSSSLNKDALWHARLGHPHGRALNLMLPG 525

Query: 159 GLLGNKQVVCTASISCLVCKLAKSKTLPFPSGAHRASNCFEMIHSDVWGMSPIASHARYK 218
            +  NK        +C  C L K     FP  +    NCF++I++D+W  +P  S   +K
Sbjct: 524 VVFENK--------NCEACILGKHCKNVFPRTSTVYENCFDLIYTDLW-TAPSLSRDNHK 372

Query: 219 YFVTFIDDYSRFTWIYFLRSKSEVFSIVITISGY 252
           YFVTFID+ S++TW+  + SK  V         Y
Sbjct: 371 YFVTFIDEKSKYTWLTLIPSKDRVIDAFKNFQAY 270


>TC93066 weakly similar to GP|19920130|gb|AAM08562.1 Putative retroelement
           {Oryza sativa} [Oryza sativa (japonica cultivar-group)],
           partial (10%)
          Length = 823

 Score = 64.3 bits (155), Expect = 4e-11
 Identities = 26/61 (42%), Positives = 37/61 (60%)
 Frame = +1

Query: 183 KTLPFPSGAHRASNCFEMIHSDVWGMSPIASHARYKYFVTFIDDYSRFTWIYFLRSKSEV 242
           K + F +  HR     + IHSD+WG S + S+   +Y +T IDD+ R  W+YFLR K+E 
Sbjct: 52  KKVSFSTATHRTKGILDYIHSDLWGPSKVTSYGGRRYMMTIIDDFPRKVWVYFLRYKNET 231

Query: 243 F 243
           F
Sbjct: 232 F 234


>AJ499215 weakly similar to GP|6642775|gb| gag-pol polyprotein {Vitis
           vinifera}, partial (18%)
          Length = 567

 Score = 49.7 bits (117), Expect = 1e-06
 Identities = 37/154 (24%), Positives = 76/154 (49%), Gaps = 7/154 (4%)
 Frame = +3

Query: 11  SRPWFLDSGASNHMTGSSEYLHNLASYHGNQQIQIADGNNLSITDVGDI----NSDFR-- 64
           S+ W +DSG ++HMT   +    L +     ++++ +G ++ +  +G +    +S ++  
Sbjct: 99  SKYWLIDSGCTHHMTHDRDLFKEL-NKSTISKVRMLNGAHIEVEGIGTVLVKSHSGYKQI 275

Query: 65  -NVLVSPGLASNLLSVGQLVDNNCNVNFSRAGCVVQEQVSGKVIAKGPKVGRLFPLQFIS 123
            NVL +P L  +LLSV QL+     V F    CV+++Q + K + +     R F L   +
Sbjct: 276 SNVLYAPKLNQSLLSVPQLLTKGYKVLFEHEKCVIKDQ-NNKEVLRAQMKARKFVLDLKN 452

Query: 124 NHLSLPCNNVLNSYEDWHRKLGHPNSTVLSHLFK 157
            +++    +     ++++ K  H ++ VL    K
Sbjct: 453 GYIT----DEDEKKKEYNFKEDHNDACVLKKFIK 542


>AW773859 
          Length = 538

 Score = 47.8 bits (112), Expect = 4e-06
 Identities = 22/68 (32%), Positives = 34/68 (49%)
 Frame = -3

Query: 140 WHRKLGHPNSTVLSHLFKTGLLGNKQVVCTASISCLVCKLAKSKTLPFPSGAHRASNCFE 199
           WH +LGH ++  L  L          +    +  C +C  ++ K LPF    +RAS C+E
Sbjct: 227 WHFRLGHLSNRKLLSLHSNFPF----ITIDQNSVCDICHYSRHKKLPFQLSTNRASKCYE 60

Query: 200 MIHSDVWG 207
           + H D+WG
Sbjct: 59  LFHFDIWG 36


>BQ154516 
          Length = 506

 Score = 35.8 bits (81), Expect = 0.017
 Identities = 16/42 (38%), Positives = 25/42 (59%)
 Frame = +3

Query: 7   SSNVSRPWFLDSGASNHMTGSSEYLHNLASYHGNQQIQIADG 48
           SS    PW+ DSGAS+H+T +   L     Y+G +Q+ + +G
Sbjct: 105 SSYSEAPWYPDSGASHHLTFNPHNLAYRTPYNGQEQVLMDNG 230


>AW736233 GP|21105451|gb small nuclear ribonucleoprotein D1 {Danio rerio},
           partial (14%)
          Length = 510

 Score = 30.4 bits (67), Expect = 0.70
 Identities = 14/33 (42%), Positives = 19/33 (57%)
 Frame = +1

Query: 7   SSNVSRPWFLDSGASNHMTGSSEYLHNLASYHG 39
           SS    PW+ DSGAS+H+T +   L     Y+G
Sbjct: 412 SSYSEAPWYPDSGASHHLTFNPHNLAYRTPYNG 510


>TC89711 weakly similar to PIR|T10725|T10725 protein kinase Xa21 (EC
           2.7.1.-) A1  receptor type - long-staminate rice,
           partial (7%)
          Length = 1391

 Score = 29.6 bits (65), Expect = 1.2
 Identities = 11/22 (50%), Positives = 13/22 (59%)
 Frame = -2

Query: 186 PFPSGAHRASNCFEMIHSDVWG 207
           PF S    A    E+IH+DVWG
Sbjct: 904 PFKSSTSHAQEVLELIHTDVWG 839


>TC84334 weakly similar to PIR|T49308|T49308 hypothetical protein T16L24.220
           - Arabidopsis thaliana, partial (14%)
          Length = 804

 Score = 28.9 bits (63), Expect = 2.0
 Identities = 17/60 (28%), Positives = 30/60 (49%)
 Frame = +1

Query: 169 TASISCLVCKLAKSKTLPFPSGAHRASNCFEMIHSDVWGMSPIASHARYKYFVTFIDDYS 228
           TA+      K + SK+LPF S  +R+          V   + IAS+A + Y  +++++ S
Sbjct: 514 TAAHDHFTLKESGSKSLPFSSHQYRSKAKMRRRRKKVEDTTDIASYAAHHYLFSYLENRS 693


>CB893545 similar to GP|8778537|gb| F22G5.5 {Arabidopsis thaliana}, partial
           (27%)
          Length = 824

 Score = 27.3 bits (59), Expect = 5.9
 Identities = 14/48 (29%), Positives = 25/48 (51%)
 Frame = -2

Query: 135 NSYEDWHRKLGHPNSTVLSHLFKTGLLGNKQVVCTASISCLVCKLAKS 182
           N   +WH+K     + V  H F+T L    + +C+A ++C  C + +S
Sbjct: 562 NDLFNWHQK----TNLVSPHAFETRLRRG*EEICSAEVTC--CNVLRS 437


>TC82987 similar to GP|15991740|gb|AAL13047.1 WRKY transcription factor 71
           {Arabidopsis thaliana}, partial (33%)
          Length = 975

 Score = 26.9 bits (58), Expect = 7.7
 Identities = 15/52 (28%), Positives = 23/52 (43%), Gaps = 11/52 (21%)
 Frame = +1

Query: 21  SNHMTGSSEYLHNLASYH-----------GNQQIQIADGNNLSITDVGDINS 61
           S+H T  S+YLH    Y+            ++ I   D NN   +  GD++S
Sbjct: 301 SSHSTSFSDYLHGSMDYNTLSKAFDLSCSSSEVISSIDDNNTKKSSAGDLSS 456


>BQ150771 GP|4028979|emb glutamate permease {synthetic construct}, partial
           (3%)
          Length = 1234

 Score = 26.9 bits (58), Expect = 7.7
 Identities = 11/32 (34%), Positives = 18/32 (55%)
 Frame = -3

Query: 157 KTGLLGNKQVVCTASISCLVCKLAKSKTLPFP 188
           ++GLL + +++C   +SCL        TLP P
Sbjct: 476 RSGLLASGRLLCLCFVSCLCIHFFSLHTLPAP 381


>TC92435 similar to GP|18377843|gb|AAL67108.1 AT5g42350/MDH9_4 {Arabidopsis
           thaliana}, partial (19%)
          Length = 611

 Score = 26.9 bits (58), Expect = 7.7
 Identities = 14/35 (40%), Positives = 23/35 (65%), Gaps = 1/35 (2%)
 Frame = -1

Query: 122 ISNHLSLPCNNV-LNSYEDWHRKLGHPNSTVLSHL 155
           + +H  L CN+V L +YED HR+L    S++L ++
Sbjct: 269 LPSH*RLICNDVSLKAYEDPHRRLIPHQSSILENV 165


  Database: MTGI
    Posted date:  Oct 22, 2004  3:39 PM
  Number of letters in database: 27,044,181
  Number of sequences in database:  36,976
  
Lambda     K      H
   0.320    0.135    0.413 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 9,101,316
Number of Sequences: 36976
Number of extensions: 138310
Number of successful extensions: 741
Number of sequences better than 10.0: 24
Number of HSP's better than 10.0 without gapping: 732
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 738
length of query: 256
length of database: 9,014,727
effective HSP length: 94
effective length of query: 162
effective length of database: 5,538,983
effective search space: 897315246
effective search space used: 897315246
frameshift window, decay const: 50,  0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 57 (26.6 bits)


Medicago: description of AC144727.5