BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC144727.4 - phase: 0
(762 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC77557 similar to PIR|JC7519|JC7519 subtilisin-like serine prot... 548 e-156
TC86329 similar to PIR|T07171|T07171 subtilisin-like proteinase ... 258 e-138
TC85583 similar to PIR|T51335|T51335 subtilisin-like proteinase ... 460 e-130
TC87377 weakly similar to PIR|T07171|T07171 subtilisin-like prot... 176 1e-87
TC83642 similar to PIR|S52769|S52769 subtilisin-like proteinase ... 188 8e-84
BG457381 weakly similar to GP|22773236|gb Putatvie subtilisin-li... 282 3e-76
TC89220 similar to PIR|T05768|T05768 subtilisin-like proteinase ... 261 5e-70
TC85585 similar to GP|22773236|gb|AAN06842.1 Putatvie subtilisin... 248 8e-66
BQ139185 weakly similar to PIR|S52769|S527 subtilisin-like prote... 243 2e-64
TC85584 weakly similar to PIR|A84473|A84473 probable serine prot... 242 3e-64
TC82946 weakly similar to GP|20160863|dbj|BAB89802. putative sub... 234 9e-62
TC81705 weakly similar to GP|6721520|dbj|BAA89562.1 EST AU029428... 231 8e-61
TC85586 similar to GP|20521377|dbj|BAB91889. putative subtilisin... 225 4e-59
TC78285 similar to PIR|A84473|A84473 probable serine proteinase ... 224 1e-58
TC91562 similar to PIR|T07172|T07172 subtilisin-like proteinase ... 171 8e-57
TC89137 similar to GP|4200338|emb|CAA76726.1 P69C protein {Lycop... 210 1e-54
TC88072 similar to PIR|T06580|T06580 subtilisin-like proteinase ... 206 3e-53
TC83163 weakly similar to GP|6721520|dbj|BAA89562.1 EST AU029428... 203 2e-52
TC81650 similar to PIR|T07171|T07171 subtilisin-like proteinase ... 197 1e-50
TC89543 similar to PIR|T06580|T06580 subtilisin-like proteinase ... 171 7e-49
>TC77557 similar to PIR|JC7519|JC7519 subtilisin-like serine proteinase (EC
3.4.21.-) - Arabidopsis thaliana, partial (88%)
Length = 2682
Score = 548 bits (1411), Expect = e-156
Identities = 333/773 (43%), Positives = 460/773 (59%), Gaps = 28/773 (3%)
Frame = +3
Query: 12 SYITSLHVIF------TLALSDNYIIHMNLSDMPKSFSNQHSWYESTLAQVTTTNNNLNN 65
S+ T++ V+F + +L YI+HM S+MP+SF + WYES+L V
Sbjct: 261 SFPTAILVLFMGLCDASSSLKSTYIVHMAKSEMPESFEHHTLWYESSLQSV--------- 413
Query: 66 STSSKIFYTYTNVMNGFSANLSPEEHESLKTFSGFISSIPDLPLKLDTTHSPQFLGLNPY 125
S S+++ YTY N ++GFS L+PEE L++ +G ++ +P++ +L TT +PQFLGL+
Sbjct: 414 SDSAEMMYTYENAIHGFSTRLTPEEARLLESQTGILAVLPEVKYELHTTRTPQFLGLDKS 593
Query: 126 RGAWPTSDFGKDIIVGVIDTGVWPESESFRDDGMTKIPSKWKGQLCQFENSNIQSINLSL 185
+P S G +++VGV+DTGVWPES+SF D G IP+ WKG C+ +N + N
Sbjct: 594 ADMFPESSSGNEVVVGVLDTGVWPESKSFNDAGFGPIPTTWKGA-CE-SGTNFTAAN--- 758
Query: 186 CNKKLIGARFFNKGFLAKHSNIS-TTILNSTRDTNGHGTHTSTTAAGSKVDGASFFGYAN 244
CNKKLIGARFF+KG A I TT S RD +GHGTHTS+TAAGS V AS FGYA+
Sbjct: 759 CNKKLIGARFFSKGVEAMLGPIDETTESKSPRDDDGHGTHTSSTAAGSVVPDASLFGYAS 938
Query: 245 GTARGIASSSRVAIYKTAWGKDGDALSSDIIAAIDAAISDGVDILSISLGSDDLLLYKDP 304
GTARG+A+ +RVA+YK W G SSDI+AAID AISD V++LS+SLG ++D
Sbjct: 939 GTARGMATRARVAVYKVCW--KGGCFSSDILAAIDKAISDNVNVLSLSLGGGMSDYFRDS 1112
Query: 305 VAIATFAAMEKGIFVSTSAGNNGPSFKSIHNGIPWVITVAAGTLDREFLGTVTLGNGVSL 364
VAI F+AMEKGI VS SAGN GPS S+ N PW+ TV AGTLDR+F +V+LGNG++
Sbjct: 1113 VAIGAFSAMEKGILVSCSAGNAGPSAYSLSNVAPWITTVGAGTLDRDFPASVSLGNGLNY 1292
Query: 365 TGLSFYLGN-FSANNFPIVFMGMCDNVKELN----------TVKRKIVVCE-GNNETLHE 412
+G+S Y GN + P+++ G N N V KIV+C+ G N +
Sbjct: 1293 SGVSLYRGNALPESPLPLIYAGNATNATNGNLCMTGTLSPELVAGKIVLCDRGMNARV-- 1466
Query: 413 QMFNVYKAKVVGGVFISNIL----DINDVDNSFPSIIINPVNGEIVKAYIKSHNSNASSI 468
Q V KA G+ +SN ++ + P+ + G +K Y+ S A
Sbjct: 1467 QKGAVVKAAGGLGMVLSNTAANGEELVADTHLLPATAVGEREGNAIKKYL---FSEAKPT 1637
Query: 469 ANMSFKKTAFGVKSTPSVDFYSSRGPSNSCPYVLKPDITAPGTSILAAWPTNV-PVSNFG 527
+ F+ T GV+ +P V +SSRGP++ P +LKPD+ APG +ILA W V P
Sbjct: 1638 VKIVFQGTKVGVEPSPVVAAFSSRGPNSITPQILKPDLIAPGVNILAGWSKAVGPTGLAV 1817
Query: 528 TEVFNNFNLIDGTSMSCPHVAGVAALLKGAHNGWSPSSIRSAIMTTSDILDNTKEHIKDI 587
E +FN+I GTSMSCPHV+G+AAL+K AH WSP+++RSA+MTT+ I ++D
Sbjct: 1818 DERRVDFNIISGTSMSCPHVSGLAALIKSAHPDWSPAAVRSALMTTAYIAYKNGNKLQDS 1997
Query: 588 GNGNRAATPFALGAGHINPNRALDPGLVYDIGVQDYINLLCALNFTQKNISAITRSSFN- 646
G +++TPF G+GH++P AL+PGLVYD+ DY+ LCALN+T I+++ R F
Sbjct: 1998 ATG-KSSTPFDHGSGHVDPVAALNPGLVYDLTADDYLGFLCALNYTATQITSLARRKFQC 2174
Query: 647 DCSK--PSLDLNYPSFIAFSNARNSSRTTNEFHRTVTNVGEKKTTYFASIT-PIKGFRVT 703
D K DLNYPSF + + + R TNVG TY AS+T K ++T
Sbjct: 2175 DAGKKYSVSDLNYPSFAVVFDTMGGANVV-KHTRIFTNVG-PAGTYKASVTSDSKNVKIT 2348
Query: 704 VIPNKLVFKKKNEKISYKLKIEGPRMTQKNKVAFGYLSWRDGKHVVRSPIVVT 756
V P +L F K NEK S+ + T + FG L W +GK+VV SPI ++
Sbjct: 2349 VEPEELSF-KANEKKSFTVTFTSSGSTPQKLNGFGRLEWTNGKNVVGSPISIS 2504
>TC86329 similar to PIR|T07171|T07171 subtilisin-like proteinase (EC
3.4.21.-) 1 - tomato, partial (91%)
Length = 2671
Score = 258 bits (659), Expect(2) = e-138
Identities = 150/323 (46%), Positives = 193/323 (59%), Gaps = 4/323 (1%)
Frame = +2
Query: 22 TLALSDNYIIHMNLSDMPKSFSNQHSWYESTLAQVTTTNNNLNNSTSSKIFYTYTNVMNG 81
T L YIIHM+ +MP SF + WY+S+L V+ T ++ YTY +V +G
Sbjct: 161 TQHLKRTYIIHMDKFNMPASFDDHLQWYDSSLKSVSDT---------AETMYTYKHVAHG 313
Query: 82 FSANLSPEEHESLKTFSGFISSIPDLPLKLDTTHSPQFLGLNPYRGAWPTSDFGKDIIVG 141
FS L+ +E + L G +S IPD+ +L TT +P+FLGL P+S ++IVG
Sbjct: 314 FSTRLTTQEADLLTKQPGILSVIPDVRYELHTTRTPEFLGLEKTITLLPSSGKQSEVIVG 493
Query: 142 VIDTGVWPESESFRDDGMTKIPSKWKGQLCQFENSNIQSINLSLCNKKLIGARFFNKGFL 201
VIDTGVWPE +SF D G+ +P WKG E ++ N S CNKKL+GARFF KG+
Sbjct: 494 VIDTGVWPELKSFDDTGLGPVPKSWKG-----ECETGKTFNSSNCNKKLVGARFFAKGYE 658
Query: 202 AKHSNI-STTILNSTRDTNGHGTHTSTTAAGSKVDGASFFGYANGTARGIASSSRVAIYK 260
A I T S RD +GHG+HTSTTAAGS V GAS FG+A+GTA+G+A+ +RVA YK
Sbjct: 659 AAFGPIDENTESKSPRDDDGHGSHTSTTAAGSAVAGASLFGFASGTAKGMATQARVAAYK 838
Query: 261 TAWGKDGDALSSDIIAAIDAAISDGVDILSIS---LGSDDLLLYKDPVAIATFAAMEKGI 317
W G +SDI AAID AI + LG YKD VA+ TFAA+E GI
Sbjct: 839 VCW--LGGCFTSDIAAAIDKAIDTQARTAAYKVSRLGGGLTDYYKDTVAMGTFAAIEHGI 1012
Query: 318 FVSTSAGNNGPSFKSIHNGIPWV 340
VS+SAGN GPS S+ N PW+
Sbjct: 1013LVSSSAGNGGPSKASLANVAPWI 1081
Score = 254 bits (649), Expect(2) = e-138
Identities = 172/437 (39%), Positives = 238/437 (54%), Gaps = 25/437 (5%)
Frame = +3
Query: 342 TVAAGTLDREFLGTVTLGNGVSLTGLSFYLGNFSANN-FPIVFMG-----MCDNVKELNT 395
TV A T+DR+F +TLGNG G+S Y G N+ P+V+ DN+ ++
Sbjct: 1086 TVGARTIDRDFPAYITLGNGNRYNGVSLYNGKLPPNSPLPLVYAANVSQDSSDNLCSTDS 1265
Query: 396 -----VKRKIVVCEGNNETLHEQMFNVYKAKVVGGVFISNILDIND--VDNSFPSIIINP 448
V KIV+C+ E+ V +A +G + ++N D + V +SF ++
Sbjct: 1266 LIPSKVSGKIVICDRGGNPRAEKSLVVKRAGGIG-MILANNQDYGEELVADSF--LLPAA 1436
Query: 449 VNGEIVKAYIKSHNSNASS-IANMSFKKTAFGVKSTPSVDFYSSRGPSNSCPYVLKPDIT 507
GE IK + S+A + A ++F T FGV+ +P V +SSRGP+ P +LKPD+
Sbjct: 1437 ALGEKASNEIKKYASSAPNPTAKIAFGGTRFGVQPSPVVAAFSSRGPNILTPKILKPDLI 1616
Query: 508 APGTSILAAWPTNVPVSNFGTEVFN-NFNLIDGTSMSCPHVAGVAALLKGAHNGWSPSSI 566
APG +ILA W V + + + +FN+I GTSMSCPHV+G+AALLKGAH WSP++I
Sbjct: 1617 APGVNILAGWSGKVGPTGLSVDTRHVSFNIISGTSMSCPHVSGLAALLKGAHPEWSPAAI 1796
Query: 567 RSAIMTTSDILDNTKEHIKDIGNGNRAATPFALGAGHINPNRALDPGLVYDIGVQDYINL 626
RSA+MTTS + IKD+ G ATP G+GH++P ALDPGLVYD DY+N
Sbjct: 1797 RSALMTTSYGTYKNGQTIKDVATG-IPATPLDYGSGHVDPVAALDPGLVYDATTDDYLNF 1973
Query: 627 LCALNFTQKNISAITRSSFNDCSKPSL----DLNYPSF-IAFSNARNSSRTTN----EFH 677
LCALN+ I + R F C K DLNYPSF + F A + N ++
Sbjct: 1974 LCALNYNSFQIKLVARREFT-CDKRIKYRVEDLNYPSFSVPFDTASGRGSSHNPSIVQYK 2150
Query: 678 RTVTNVGEKKTTYFASITPIKGF-RVTVIPNKLVFKKKNEKISYKLKIEGPRMTQKNKVA 736
R +TNVG +TY S++ ++ V P L FK+ NEK SY + M +
Sbjct: 2151 RILTNVG-APSTYKVSVSSQSPLDKIVVEPQTLSFKELNEKKSYTVTFTSHSM-PSGTTS 2324
Query: 737 FGYLSWRDGKHVVRSPI 753
F +L W DGKH V SPI
Sbjct: 2325 FAHLEWSDGKHKVTSPI 2375
>TC85583 similar to PIR|T51335|T51335 subtilisin-like proteinase AIR3
auxin-induced [imported] - Arabidopsis thaliana
(fragment), partial (40%)
Length = 2691
Score = 460 bits (1184), Expect = e-130
Identities = 291/738 (39%), Positives = 407/738 (54%), Gaps = 53/738 (7%)
Frame = +2
Query: 71 IFYTYTNVMNGFSANLSPEEHESLKTFSGFISSIPDLPLKLDTTHSPQFLGL--NPYRGA 128
I Y+Y +NGF+A L EE + +S P KL TT S +FLGL N A
Sbjct: 251 IIYSYNKHINGFAALLEDEEAADIAKKRNVVSVFLSKPHKLHTTRSWEFLGLRRNAKNTA 430
Query: 129 WPTSDFGKDIIVGVIDTGVWPESESFRDDGMTKIPSKWKG-QLCQFENSNIQSINLSLCN 187
W FG++ I+ IDTGVWPES+SF D G +PSKW+G + C+ S + CN
Sbjct: 431 WQKGKFGENTIIANIDTGVWPESKSFNDKGYGPVPSKWRGGKACEI--SKFSKYKKNPCN 604
Query: 188 KKLIGARFFNKGFLAKHSNISTTILNSTRDTNGHGTHTSTTAAGSKVDGASFFGYANGTA 247
+KLIGARFF+ + A + + + + RD GHGTHT +TA G+ V AS F NGT
Sbjct: 605 RKLIGARFFSNAYEAYNDKLPSW-QRTARDFLGHGTHTLSTAGGNFVPDASVFAIGNGTV 781
Query: 248 RGIASSSRVAIYKTAWGKDG--DALSSDIIAAIDAAISDGVDILSISLGSDDLL----LY 301
+G + +RVA YK W D +D++AAID AISDGVDI+S+SL L+ ++
Sbjct: 782 KGGSPRARVATYKVCWSLLDLEDCFGADVLAAIDQAISDGVDIISLSLAGHSLVYPEDIF 961
Query: 302 KDPVAIATFAAMEKGIFVSTSAGNNGPSFKSIHNGIPWVITVAAGTLDREFLGTVTLGNG 361
D V+I F A+ + I + SAGN GP+ S+ N PWV T+AA TLDR+F T+T+GN
Sbjct: 962 TDEVSIGAFHALSRNILLVASAGNEGPTGGSVVNVAPWVFTIAASTLDRDFSSTITIGNQ 1141
Query: 362 VSLTGLSFYLGNFSANNFPIVFM--GMCDNVK------------ELNTVKRKIVVC--EG 405
++ G S ++ FP++ G N + + VK KIV C EG
Sbjct: 1142-TIRGASLFVNLPPNQAFPLIVSTDGKLANATNHDAQFCKPGTLDPSKVKGKIVECIREG 1318
Query: 406 NNETLHEQMFNVYKAKVVG--GVFISN-------------ILDINDVDNSFPSIIINPVN 450
N +++ E +A G G+ +SN L +V + P +
Sbjct: 1319NIKSVAEGQ----EALSAGAKGMLLSNQPKQGKTTLAEPHTLSCVEVPHHAPKPPKPKKS 1486
Query: 451 GEIVKAY-------IKSHNSNASSIANMSFK--KTAFGVKSTPSVDFYSSRGPSNSCPYV 501
E +A I S +S + + F KT +G K P + +SSRGP+ P +
Sbjct: 1487AEQERAGSHAPAFDITSMDSKLKAGTTIKFSGAKTLYGRKPAPVMASFSSRGPNKIQPSI 1666
Query: 502 LKPDITAPGTSILAAWPTNVPVSNFGTEVFNNF--NLIDGTSMSCPHVAGVAALLKGAHN 559
LKPD+TAPG +ILAA+ SN T+ NNF N++ GTSMSCPHVAG+A L+K H
Sbjct: 1667LKPDVTAPGVNILAAYSLYASASNLKTDNRNNFPFNVLQGTSMSCPHVAGIAGLIKTLHP 1846
Query: 560 GWSPSSIRSAIMTTSDILDNTKEHIKDIGNGNRAATPFALGAGHINPNRALDPGLVYDIG 619
WSP++I+SAIMTT+ LDNT I+D N+ A PF G+GH+ P+ A+DPGLVYD+G
Sbjct: 1847NWSPAAIKSAIMTTATTLDNTNRPIQDAFE-NKLAIPFDYGSGHVQPDLAIDPGLVYDLG 2023
Query: 620 VQDYINLLCALNFTQKNISAITRSSFNDC--SKPSLDLNYPSFIAFSNARNSSRTTNEFH 677
++DY+N LCA + Q+ ISA+ + C S D NYPS + N+ T
Sbjct: 2024IKDYLNFLCAYGYNQQLISALNFNGTFICSGSHSITDFNYPSITLPNLKLNAVNVT---- 2191
Query: 678 RTVTNVGEKKTTYFASITPIKGFRVTVIPNKLVFKKKNEKISYKLKIEGPRMTQKNKVAF 737
RTVTNVG T +++ + G+++ V+PN L FKK EK ++++ ++ +T + K F
Sbjct: 2192RTVTNVGPPGT--YSAKAQLLGYKIVVLPNSLTFKKTGEKKTFQVIVQATNVTPRGKYQF 2365
Query: 738 GYLSWRDGKHVVRSPIVV 755
G L W DGKH+VRSPI V
Sbjct: 2366GNLQWTDGKHIVRSPITV 2419
>TC87377 weakly similar to PIR|T07171|T07171 subtilisin-like proteinase (EC
3.4.21.-) 1 - tomato, partial (54%)
Length = 2071
Score = 176 bits (445), Expect(2) = 1e-87
Identities = 102/233 (43%), Positives = 133/233 (56%), Gaps = 12/233 (5%)
Frame = +1
Query: 533 NFNLIDGTSMSCPHVAGVAALLKGAHNGWSPSSIRSAIMTTSDILDNTKEHIKDIGNGNR 592
NFN+I GTSMSCPH +G+AA++KGA+ WSP++IRSA+MTT+ + I D+ G +
Sbjct: 880 NFNIISGTSMSCPHASGLAAIVKGAYPEWSPAAIRSALMTTAYTSYKNGQTIVDVATG-K 1056
Query: 593 AATPFALGAGHINPNRALDPGLVYDIGVQDYINLLCALNFTQKNISAITRSSFNDCSKPS 652
ATPF G+GH++P ALDPGLVYDI V DY+ CALN+T I R F ++
Sbjct: 1057 PATPFDFGSGHVDPVSALDPGLVYDINVDDYLGFFCALNYTSYQIKLAARREFTCDARKK 1236
Query: 653 L---DLNYPSF-IAFSNARNSSRTTN-----EFHRTVTNVGEKKT---TYFASITPIKGF 700
D NYPSF +A A +N E++R +TNVG T T S
Sbjct: 1237 YRVEDFNYPSFAVALETASGIGGGSNKPIIVEYNRVLTNVGAPGTYNATVVLSSVDSSSV 1416
Query: 701 RVTVIPNKLVFKKKNEKISYKLKIEGPRMTQKNKVAFGYLSWRDGKHVVRSPI 753
+V V P + FK+ EK YK++ M K +FGYL W DGKH V SPI
Sbjct: 1417 KVVVEPETISFKEVYEKKGYKVRFICGSMPSGTK-SFGYLEWNDGKHKVGSPI 1572
Score = 166 bits (421), Expect(2) = 1e-87
Identities = 106/282 (37%), Positives = 156/282 (54%), Gaps = 17/282 (6%)
Frame = +2
Query: 251 ASSSRVAIYKTAWGKDGDALSSDIIAAIDAAISDGVDILSISLGSDDLLLYKDPVAIATF 310
A+ +RVA YK W +SDI A +D AI DGV+ILS+S+G + Y+D +AI F
Sbjct: 2 ATEARVAAYKVCWLSG--CFTSDIAAGMDKAIEDGVNILSMSIGGSIMDYYRDIIAIGAF 175
Query: 311 AAMEKGIFVSTSAGNNGPSFKSIHNGIPWVITVAAGTLDREFLGTVTLGNGVSLTGLSFY 370
AM GI VS+SAGN GPS +S+ N PW+ TV AGT+DR+F +TLGNG + TG S Y
Sbjct: 176 TAMSHGILVSSSAGNGGPSAESLSNVAPWITTVGAGTIDRDFPSYITLGNGKTYTGASLY 355
Query: 371 LGNFSANN-FPIVFMGMCDNVKELNT-------------VKRKIVVCEGNNETLHEQMFN 416
G S+++ P+V+ G NV E + V KIV+CE + E+
Sbjct: 356 NGKPSSDSLLPVVYAG---NVSESSVGYLCIPDSLTSSKVLGKIVICERGGNSRVEKGLV 526
Query: 417 VYKAKVVGGVFISNIL---DINDVDNSFPSIIINPVNGEIVKAYIKSHNSNASSIANMSF 473
V A VG + ++N ++ + P+ + + ++K Y+ + + A + F
Sbjct: 527 VKNAGGVGMILVNNEAYGEELIADSHLLPAAALGQKSSTVLKDYV---FTTKNPRAKLVF 697
Query: 474 KKTAFGVKSTPSVDFYSSRGPSNSCPYVLKPDITAPGTSILA 515
T V+ +P V +SSRGP++ P +LKPD+ APG +ILA
Sbjct: 698 GGTHLQVQPSPVVAAFSSRGPNSLTPKILKPDLIAPGVNILA 823
>TC83642 similar to PIR|S52769|S52769 subtilisin-like proteinase ag12 (EC
3.4.21.-) - alder, partial (30%)
Length = 1177
Score = 188 bits (478), Expect(2) = 8e-84
Identities = 92/164 (56%), Positives = 122/164 (74%)
Frame = +3
Query: 532 NNFNLIDGTSMSCPHVAGVAALLKGAHNGWSPSSIRSAIMTTSDILDNTKEHIKDIGNGN 591
+++N + GTSMSCPHV+GVAALLK AH WS ++IRSA++TT++ LDNT+ I+D G +
Sbjct: 618 SDYNFMSGTSMSCPHVSGVAALLKAAHPQWSAAAIRSALITTANPLDNTQNPIRDNGYPS 797
Query: 592 RAATPFALGAGHINPNRALDPGLVYDIGVQDYINLLCALNFTQKNISAITRSSFNDCSKP 651
+ A+P A+GAG I+PNRA++PGL+YD QDY+NLLC L FT+ I ITRS+ DC P
Sbjct: 798 QHASPLAIGAGEIDPNRAMNPGLIYDATPQDYVNLLCGLKFTKNQILTITRSNSYDCENP 977
Query: 652 SLDLNYPSFIAFSNARNSSRTTNEFHRTVTNVGEKKTTYFASIT 695
SLDLNYPSFIAF + + S ++F R VTNVG+ TY A +T
Sbjct: 978 SLDLNYPSFIAFYSNKTRS-MVHKFKRIVTNVGDGAATYRAKVT 1106
Score = 141 bits (355), Expect(2) = 8e-84
Identities = 79/205 (38%), Positives = 127/205 (61%), Gaps = 10/205 (4%)
Frame = +2
Query: 335 NGIPWVITVAAGTLDREFLGTVTLGNGVSLTGLSFYLGNFSANNFPIVF---MGMCDNVK 391
NGIPW++T AAGT+DR F GT+ LGNG S+ G + + N N +V+ + C+++
Sbjct: 2 NGIPWLLTAAAGTIDRTF-GTLVLGNGQSIIGWTLFPANAIVENVLLVYNNTLSSCNSLN 178
Query: 392 ELNTVKRKIVV-CEG-----NNETLHEQMFNVYKAKVVGGVFISNILDINDVDNSF-PSI 444
L+ + +K+++ C+ N ++ Q+ V +A ++G VF+S+ + D+ + PSI
Sbjct: 179 LLSQLNKKVIILCDDSLSNRNKTSVFNQINVVTEANLLGAVFVSDSPQLIDLGRIYTPSI 358
Query: 445 IINPVNGEIVKAYIKSHNSNASSIANMSFKKTAFGVKSTPSVDFYSSRGPSNSCPYVLKP 504
+I P + + V Y KS+N+ SSI F++T G K P+ +YSSRGPS+S P++LKP
Sbjct: 359 VIKPKDAQSVINYAKSNNNPTSSI---KFQQTFVGTKPAPAAAYYSSRGPSHSYPWILKP 529
Query: 505 DITAPGTSILAAWPTNVPVSNFGTE 529
DI APG+ +LAA+ N P + GT+
Sbjct: 530 DIMAPGSRVLAAYIPNKPTARIGTK 604
>BG457381 weakly similar to GP|22773236|gb Putatvie subtilisin-like serine
protease {Oryza sativa (japonica cultivar-group)},
partial (15%)
Length = 660
Score = 282 bits (722), Expect = 3e-76
Identities = 143/196 (72%), Positives = 165/196 (83%), Gaps = 1/196 (0%)
Frame = +2
Query: 421 KVVGGVFISNILDINDVDNSFPSIIINPVNGEIVKAYIKSHNSNASSIANMSFKKTAFGV 480
K + V ISN+ DI NSF +I++NP+NGEIVKAYIKS SN++SIA+MSF KT FG
Sbjct: 62 KFLEXVLISNVSDIFYSQNSFATILVNPINGEIVKAYIKS--SNSTSIASMSFMKTVFGT 235
Query: 481 KSTPSVDFYSSRGPSNSCPYVLKPDITAPGTSILAAWPTNVPVSNFGT-EVFNNFNLIDG 539
K PSVD YSSRGPS SCP+VLKPDITAPGTSILAAWPTNVPV F + ++F+ FNLI G
Sbjct: 236 KPAPSVDSYSSRGPSYSCPFVLKPDITAPGTSILAAWPTNVPVMEFESHKIFSKFNLISG 415
Query: 540 TSMSCPHVAGVAALLKGAHNGWSPSSIRSAIMTTSDILDNTKEHIKDIGNGNRAATPFAL 599
TSM+CPHVAGVAALLKG H+ WSP++IRSAIMTTSDI DN+KE IKDIG ++AATPFAL
Sbjct: 416 TSMACPHVAGVAALLKGVHSDWSPAAIRSAIMTTSDIFDNSKEQIKDIGT-DKAATPFAL 592
Query: 600 GAGHINPNRALDPGLV 615
GAGH+NPNRALDPGLV
Sbjct: 593 GAGHVNPNRALDPGLV 640
>TC89220 similar to PIR|T05768|T05768 subtilisin-like proteinase (EC
3.4.21.-) - Arabidopsis thaliana, partial (39%)
Length = 1149
Score = 261 bits (668), Expect = 5e-70
Identities = 143/361 (39%), Positives = 207/361 (56%), Gaps = 12/361 (3%)
Frame = +3
Query: 4 NICLWLWFSYITSLHVIFTL--ALSDNYIIHMNLSDMPKSFSNQHSWYESTLAQVTTTNN 61
N ++ F + SL+ + + L +I +N P F + WY + + T
Sbjct: 57 NSIVFFLFFFSLSLNQVLSSDEELPKTFIFRVNSYSKPSIFPTHYHWYTAEFTEQTN--- 227
Query: 62 NLNNSTSSKIFYTYTNVMNGFSANLSPEEHESLKTFSGFISSIPDLPLKLDTTHSPQFLG 121
I +TY +GFSA L+ ++ S+ ++ D +L TT SPQFLG
Sbjct: 228 ---------ILHTYDTAFHGFSAVLTRQQVASISNHPSILAVFEDRRRQLHTTRSPQFLG 380
Query: 122 LNPYRGAWPTSDFGKDIIVGVIDTGVWPESESFRDDGMTKIPSKWKGQLCQFENSNIQSI 181
L RG W SD+G D+IVGV DTG+WPE SF D + IP +WKG +C+ + +
Sbjct: 381 LRNQRGLWSESDYGSDVIVGVFDTGIWPERRSFSDMNLGPIPRRWKG-VCE----SGEKF 545
Query: 182 NLSLCNKKLIGARFFNKGFLAKHSNI-------STTILNSTRDTNGHGTHTSTTAAGSKV 234
+ CN+KLIGAR+F+KG + T S RD +GHGTHT++TAAG
Sbjct: 546 SPRNCNRKLIGARYFSKGHEVGAGSAGPLNPINETVEFRSPRDADGHGTHTASTAAGRYA 725
Query: 235 DGASFFGYANGTARGIASSSRVAIYKTAWGKDGDALSSDIIAAIDAAISDGVDILSISLG 294
A+ GYA+G A+G+A +R+A+YK W K+ SDI+AA DAA++DGVD++SIS+G
Sbjct: 726 FQANMSGYASGIAKGVAPKARLAVYKVCW-KNSGCFDSDILAAFDAAVNDGVDVISISIG 902
Query: 295 SDDLL---LYKDPVAIATFAAMEKGIFVSTSAGNNGPSFKSIHNGIPWVITVAAGTLDRE 351
D + Y DP+AI ++ A+ +G+FVS+SAGN+GPS S+ N PW+ TV AGT+DR+
Sbjct: 903 GGDGIASPYYLDPIAIGSYGAVSRGVFVSSSAGNDGPSGMSVTNLAPWLTTVGAGTIDRD 1082
Query: 352 F 352
F
Sbjct: 1083F 1085
>TC85585 similar to GP|22773236|gb|AAN06842.1 Putatvie subtilisin-like
serine protease {Oryza sativa (japonica
cultivar-group)}, partial (21%)
Length = 1353
Score = 248 bits (632), Expect = 8e-66
Identities = 141/333 (42%), Positives = 196/333 (58%), Gaps = 4/333 (1%)
Frame = +2
Query: 434 INDVDNSFPSIIINPVNGEIVKAYIKSHNSNASSIANMSFKKTAFGVKSTPSVDFYSSRG 493
++ ++ P I P EI IK++ + S AN G K P + +SSRG
Sbjct: 8 VSTINYHDPRSITTPKGSEITPEDIKTNATIRMSPAN-----ALNGRKPAPVMASFSSRG 172
Query: 494 PSNSCPYVLKPDITAPGTSILAAWPTNVPVSNFGTEVFNNF--NLIDGTSMSCPHVAGVA 551
P+ PY+LKPD+TAPG +ILAA+ VSN T+ F N+ GTSMSCPHV G A
Sbjct: 173 PNKVQPYILKPDVTAPGVNILAAYSLLASVSNLVTDNRRGFPFNIQQGTSMSCPHVVGTA 352
Query: 552 ALLKGAHNGWSPSSIRSAIMTTSDILDNTKEHIKDIGNGNRAATPFALGAGHINPNRALD 611
L+K H WSP++I+SAIMTT+ DNT E I+D N A FA G+GHI PN A+D
Sbjct: 353 GLIKTLHPNWSPAAIKSAIMTTATTRDNTNEPIEDAFE-NTTANAFAYGSGHIQPNSAID 529
Query: 612 PGLVYDIGVQDYINLLCALNFTQKNISAITRSSFNDC--SKPSLDLNYPSFIAFSNARNS 669
PGLVYD+G++DY+N LCA + QK IS++ + C ++ DLNYPS + N+
Sbjct: 530 PGLVYDLGIKDYLNFLCAAGYNQKLISSLIFNMTFTCYGTQSINDLNYPSITLPNLGLNA 709
Query: 670 SRTTNEFHRTVTNVGEKKTTYFASITPIKGFRVTVIPNKLVFKKKNEKISYKLKIEGPRM 729
T RTVTNVG + T + + + G+++ V+P+ L FKK EK ++K+ ++ +
Sbjct: 710 VTVT----RTVTNVGPRST--YTAKAQLPGYKIVVVPSSLKFKKIGEKKTFKVTVQATSV 871
Query: 730 TQKNKVAFGYLSWRDGKHVVRSPIVVTNINFNL 762
T + K FG L W +GKH+VRSPI + N NL
Sbjct: 872 TPQGKYEFGELQWSNGKHIVRSPITLRRENTNL 970
>BQ139185 weakly similar to PIR|S52769|S527 subtilisin-like proteinase ag12
(EC 3.4.21.-) - alder, partial (11%)
Length = 667
Score = 243 bits (621), Expect = 2e-64
Identities = 131/224 (58%), Positives = 164/224 (72%), Gaps = 7/224 (3%)
Frame = +2
Query: 1 MASNICLWLWFSYITSLHVIFTLALSD----NYIIHMNLSDMPKSFSNQHSWYESTLAQ- 55
MAS+I +L S+ LH +F+ L D NYIIHMNLS MPK F +Q SWY +TL+
Sbjct: 14 MASHIHHYLLLSFNIILH-LFSSTLCDQNFNNYIIHMNLSAMPKPFLSQQSWYLATLSSL 190
Query: 56 --VTTTNNNLNNSTSSKIFYTYTNVMNGFSANLSPEEHESLKTFSGFISSIPDLPLKLDT 113
+T+ N+ L+ S K+ YTYTNVMNGFSA+LSP E E+LKT G+ISSI DLP+K DT
Sbjct: 191 LDITSNNDQLSYIFSPKLTYTYTNVMNGFSASLSPLELEALKTTPGYISSIRDLPIKPDT 370
Query: 114 THSPQFLGLNPYRGAWPTSDFGKDIIVGVIDTGVWPESESFRDDGMTKIPSKWKGQLCQF 173
THSP F+GLNP G WPT+ +GK+II+G+ID+G+WPESESF+DD M IPS+WKG+ C+
Sbjct: 371 THSPHFIGLNPVFGTWPTTQYGKNIIIGLIDSGIWPESESFKDDEMPNIPSRWKGK-CE- 544
Query: 174 ENSNIQSINLSLCNKKLIGARFFNKGFLAKHSNISTTILNSTRD 217
N + SLCNKKLIGARFFNKG LA + NI+ T +NSTRD
Sbjct: 545 ---NGTQFDSSLCNKKLIGARFFNKGLLANNPNITIT-MNSTRD 664
>TC85584 weakly similar to PIR|A84473|A84473 probable serine proteinase
[imported] - Arabidopsis thaliana, partial (19%)
Length = 1241
Score = 242 bits (618), Expect = 3e-64
Identities = 135/289 (46%), Positives = 181/289 (61%), Gaps = 4/289 (1%)
Frame = +1
Query: 471 MSFKKTAFGVKSTPSVDFYSSRGPSNSCPYVLKPDITAPGTSILAAWPTNVPVSNFGTEV 530
MS KT K P + YSSRGP+ P +LKPD+TAPG +ILAA+ SN T+
Sbjct: 211 MSPAKTLNRRKPAPVMASYSSRGPNKVQPSILKPDVTAPGVNILAAYSLFASASNLITDT 390
Query: 531 FNNF--NLIDGTSMSCPHVAGVAALLKGAHNGWSPSSIRSAIMTTSDILDNTKEHIKDIG 588
F N++ GTSMSCPHVAG A L+K H WSP++I+SAIMTT+ DNT + I D
Sbjct: 391 RRGFPFNVMQGTSMSCPHVAGTAGLIKTLHPNWSPAAIKSAIMTTATTRDNTNKPISDAF 570
Query: 589 NGNRAATPFALGAGHINPNRALDPGLVYDIGVQDYINLLCALNFTQKNISAITRSSFNDC 648
+ A PFA G+GHI PN A+DPGLVYD+G++DY+N LCA + Q+ ISA+ + C
Sbjct: 571 D-KTLANPFAYGSGHIRPNSAMDPGLVYDLGIKDYLNFLCASGYNQQLISALNFNMTFTC 747
Query: 649 SKPSL--DLNYPSFIAFSNARNSSRTTNEFHRTVTNVGEKKTTYFASITPIKGFRVTVIP 706
S S DLNYPS + NS T RTVTNVG +TYFA + + G+++ V+P
Sbjct: 748 SGTSSIDDLNYPSITLPNLGLNSVTVT----RTVTNVG-PPSTYFAKV-QLAGYKIAVVP 909
Query: 707 NKLVFKKKNEKISYKLKIEGPRMTQKNKVAFGYLSWRDGKHVVRSPIVV 755
+ L FKK EK ++++ ++ +T + K FG L W +GKH+VRSP+ V
Sbjct: 910 SSLNFKKIGEKKTFQVIVQATSVTPRRKYQFGELRWTNGKHIVRSPVTV 1056
>TC82946 weakly similar to GP|20160863|dbj|BAB89802. putative
subtilisin-like protease {Oryza sativa (japonica
cultivar-group)}, partial (21%)
Length = 921
Score = 234 bits (597), Expect = 9e-62
Identities = 120/186 (64%), Positives = 147/186 (78%), Gaps = 7/186 (3%)
Frame = +3
Query: 11 FSYITSLHVIF-TLALSDNYIIHMNLSDMPKSFSNQHSWYESTLA------QVTTTNNNL 63
FS IT LH++F T+A SDNYIIHM+ + MPK F+ H+WY STL+ Q+TT++NNL
Sbjct: 9 FSCITFLHLLFITIAQSDNYIIHMDKAAMPKVFTTHHTWYLSTLSSALENPQLTTSSNNL 188
Query: 64 NNSTSSKIFYTYTNVMNGFSANLSPEEHESLKTFSGFISSIPDLPLKLDTTHSPQFLGLN 123
N+ SSK+ YTYT+VMNGFSANLSP+EHESLKT G+ISSI D +KLDTTHSPQFLGLN
Sbjct: 189 NSLFSSKLIYTYTHVMNGFSANLSPKEHESLKTSPGYISSIKDSHMKLDTTHSPQFLGLN 368
Query: 124 PYRGAWPTSDFGKDIIVGVIDTGVWPESESFRDDGMTKIPSKWKGQLCQFENSNIQSINL 183
P +GAW S+FG D+IVG+IDTG+WPESESF+D+ M++IPSKW GQ ENS + N
Sbjct: 369 PNKGAWHDSNFGNDVIVGLIDTGIWPESESFKDNLMSEIPSKWNGQC---ENSIL--FNS 533
Query: 184 SLCNKK 189
SLCNKK
Sbjct: 534 SLCNKK 551
Score = 169 bits (427), Expect = 5e-42
Identities = 92/136 (67%), Positives = 109/136 (79%)
Frame = +1
Query: 180 SINLSLCNKKLIGARFFNKGFLAKHSNISTTILNSTRDTNGHGTHTSTTAAGSKVDGASF 239
SI+L + LIGA+FFNKG +K+ NI T LNSTRD++GHGTHTS+TAAG++VD ASF
Sbjct: 526 SIHL-FATRNLIGAKFFNKGLQSKYPNI-TLGLNSTRDSHGHGTHTSSTAAGNRVDDASF 699
Query: 240 FGYANGTARGIASSSRVAIYKTAWGKDGDALSSDIIAAIDAAISDGVDILSISLGSDDLL 299
FGYA GTA GIAS+SRVA+YK W D LSSD+IAAIDAAISDGVD+LS+S G +D+
Sbjct: 700 FGYAPGTASGIASNSRVAMYKAIW--DVGVLSSDVIAAIDAAISDGVDVLSLSFGINDVP 873
Query: 300 LYKDPVAIATFAAMEK 315
LY PVAIATFA MEK
Sbjct: 874 LYDXPVAIATFAXMEK 921
>TC81705 weakly similar to GP|6721520|dbj|BAA89562.1 EST AU029428(E30359)
corresponds to a region of the predicted gene.~Similar
to antifreeze-like, partial (33%)
Length = 1443
Score = 231 bits (589), Expect = 8e-61
Identities = 134/315 (42%), Positives = 183/315 (57%), Gaps = 9/315 (2%)
Frame = +1
Query: 70 KIFYTYTNVMNGFSANLSPEEHESLKTFSGFISSIPDLPLKLDTTHSPQFL----GLNPY 125
K+ + Y + +GFSA L+ E +L G +S PD L+L TT S FL G+ P
Sbjct: 196 KLIHHYNHAFSGFSAMLTQSEASALSGHDGVVSVFPDPILELHTTRSWDFLDSDLGMKPS 375
Query: 126 RGAWPTSDFGKDIIVGVIDTGVWPESESFRDDGMTKIPSKWKGQLCQFENSNIQSINLSL 185
DII+ +IDTG+WPES SF D+G+ KIPS WKG +C E + + N
Sbjct: 376 TNVLTHQHSSNDIIIALIDTGIWPESPSFTDEGIGKIPSVWKG-ICM-EGHDFKKSN--- 540
Query: 186 CNKKLIGARFFNKG--FLAKHSNISTTILNSTRDTNGHGTHTSTTAAGSKVDGASFFGYA 243
CN+KLIGAR++N F + ++I S RDT GHGTHT++TAAG V+ A+++G A
Sbjct: 541 CNRKLIGARYYNTQDTFGSNKTHIGGA-KGSPRDTVGHGTHTASTAAGVNVNNANYYGLA 717
Query: 244 NGTARGIASSSRVAIYKTAWGKDGDALSSDIIAAIDAAISDGVDILSISLGSDDLL---L 300
GTARG + S+R+A YKT + S I+ A+D AI DGVDI+SIS+G L+
Sbjct: 718 KGTARGGSPSTRIAAYKTC--SEEGCSGSTILKAMDDAIKDGVDIISISIGLSSLMQSDY 891
Query: 301 YKDPVAIATFAAMEKGIFVSTSAGNNGPSFKSIHNGIPWVITVAAGTLDREFLGTVTLGN 360
DP+AI F A ++G+ SAGN+GP ++ N PW+ TVAA +DR F T+ LGN
Sbjct: 892 LNDPIAIGAFHAEQRGVTAVCSAGNDGPDPNTVVNTAPWIFTVAASNIDRNFQSTIVLGN 1071
Query: 361 GVSLTGLSFYLGNFS 375
G S G N +
Sbjct: 1072GKSFQGAGINFSNLT 1116
>TC85586 similar to GP|20521377|dbj|BAB91889. putative subtilisin-like
protease {Oryza sativa (japonica cultivar-group)},
partial (17%)
Length = 1460
Score = 225 bits (574), Expect = 4e-59
Identities = 145/377 (38%), Positives = 208/377 (54%), Gaps = 17/377 (4%)
Frame = +1
Query: 396 VKRKIVVC--EGNNETLHEQ----------MFNVYKAKVVGGVFISNILDINDVDNSFPS 443
VK KIV C EG +++ E MF + KV G +S ++ V + +
Sbjct: 76 VKGKIVACAREGKIKSVAEGQEALSAGAKGMFLENQPKVSGNTLLSEPHVLSTVGGNGQA 255
Query: 444 IIINPVNGEIVKAYIKSHNSNASSIANMSFKKTAFGVKSTPSVDFYSSRGPSNSCPYVLK 503
I P + + + + S T G K P + +SSRGP+ PY+LK
Sbjct: 256 AITAPPRLGVTA----TDTIESGTKIRFSQAITLIGRKPAPVMASFSSRGPNQVQPYILK 423
Query: 504 PDITAPGTSILAAWPTNVPVSNFGTEVFNNF--NLIDGTSMSCPHVAGVAALLKGAHNGW 561
PD+TAPG +ILAA+ SN T+ F N++ GTSMSCPHVAG A L+K H W
Sbjct: 424 PDVTAPGVNILAAYSLFASASNLLTDNRRGFPFNVMQGTSMSCPHVAGTAGLIKTLHPNW 603
Query: 562 SPSSIRSAIMTTSDILDNTKEHIKDIGNGNRAATPFALGAG-HINPNRALDPGLVYDIGV 620
SP++I+SAIMTT+ DNT + I D + A PFA G+G + +P A+DPGLVYD+G+
Sbjct: 604 SPAAIKSAIMTTATTRDNTNKPISDAFD-KTLADPFAYGSGTYSHPTSAIDPGLVYDLGI 780
Query: 621 QDYINLLCALNFTQKNISAITRSSFNDCSKPSL--DLNYPSFIAFSNARNSSRTTNEFHR 678
+DY+N LCA + ++ ISA+ + CS DLNYPS + N+ T R
Sbjct: 781 KDYLNFLCASGYNKQLISALNFNMTFTCSGTHSIDDLNYPSITLPNLGLNAITVT----R 948
Query: 679 TVTNVGEKKTTYFASITPIKGFRVTVIPNKLVFKKKNEKISYKLKIEGPRMTQKNKVAFG 738
TVTNVG +TYFA + + G+++ V+P+ L FKK EK ++++ ++ + K FG
Sbjct: 949 TVTNVG-PPSTYFAKV-QLPGYKIAVVPSSLNFKKIGEKKTFQVIVQATSEIPRRKYQFG 1122
Query: 739 YLSWRDGKHVVRSPIVV 755
L W +GKH+VRSP+ V
Sbjct: 1123ELRWTNGKHIVRSPVTV 1173
>TC78285 similar to PIR|A84473|A84473 probable serine proteinase [imported]
- Arabidopsis thaliana, partial (41%)
Length = 1353
Score = 224 bits (570), Expect = 1e-58
Identities = 126/319 (39%), Positives = 188/319 (58%), Gaps = 7/319 (2%)
Frame = +1
Query: 442 PSIIINPVNGEIVKAYIKSHNSNASSIANMSFKKTAFGVKSTPSVDFYSSRGPSNSCPYV 501
P++ + + G+ ++ Y+ S + N +++ +SF T V+ +P V +SSRGP+ +
Sbjct: 109 PAVAVGRIIGDQIRKYVSS-DLNPTTV--LSFGGTVLNVRPSPVVAAFSSRGPNMITKEI 279
Query: 502 LKPDITAPGTSILAAWPTNVPVSNFGTEVFNN-FNLIDGTSMSCPHVAGVAALLKGAHNG 560
LKPD+ PG +ILA W V S + FN++ GTSMSCPH++G+AALLK AH
Sbjct: 280 LKPDVIGPGVNILAGWSEAVGPSGLAEDTRKTKFNIMSGTSMSCPHISGLAALLKAAHPT 459
Query: 561 WSPSSIRSAIMTTSDILDNTKEHIKDIGNGNRAATPFALGAGHINPNRALDPGLVYDIGV 620
WSPS+I+SA+MTT+ DN+K ++D +G+ +TP A GAGH+NP +AL PGLVYD
Sbjct: 460 WSPSAIKSALMTTAYNHDNSKSPLRDAADGS-FSTPLAHGAGHVNPQKALSPGLVYDAST 636
Query: 621 QDYINLLCALNFTQKNISAITRSSFNDCSKPSLD---LNYPSF-IAFSNARNSSRTTNEF 676
+DYI LC+LN+ + I I + +C+K + LNYPSF + F SS+ +
Sbjct: 637 KDYITFLCSLNYNSEQIQLIVKRPSVNCTKKFANPGQLNYPSFSVVF-----SSKRVVRY 801
Query: 677 HRTVTNVGEKKTTYFASITPIKGFRVTVIPNKLVFKKKNEKISYKLKIEGPRMTQKNKV- 735
R VTNVGE + Y + +TV P++LVF+K E+ Y + + +KV
Sbjct: 802 TRIVTNVGEAGSVYNVVVDVPSSVGITVKPSRLVFEKVGERKRYTVTFVSKKGADASKVR 981
Query: 736 -AFGYLSWRDGKHVVRSPI 753
FG + W + +H VRSPI
Sbjct: 982 SGFGSILWSNAQHQVRSPI 1038
>TC91562 similar to PIR|T07172|T07172 subtilisin-like proteinase (EC
3.4.21.-) 2 - tomato, partial (36%)
Length = 1062
Score = 171 bits (433), Expect(2) = 8e-57
Identities = 87/199 (43%), Positives = 128/199 (63%), Gaps = 8/199 (4%)
Frame = +2
Query: 469 ANMSFKKTAFGVKSTPSVDFYSSRGPSNSCPYVLKPDITAPGTSILAAW-----PTNVPV 523
A ++F T G++ +P V +SSRGPS +LKPDI APG +ILAAW P+++P+
Sbjct: 8 ATLAFHNTRLGIRPSPIVAAFSSRGPSLLTLEILKPDIVAPGVNILAAWSGLTGPSSLPI 187
Query: 524 SNFGTEVFNNFNLIDGTSMSCPHVAGVAALLKGAHNGWSPSSIRSAIMTTSDILDNTKEH 583
+ + FN++ GTSMSCPHV+G+AA++K H WSP++I+SAIMTT+ + DNT +
Sbjct: 188 DHRRVK----FNILSGTSMSCPHVSGIAAMIKAKHPEWSPAAIKSAIMTTAYVHDNTIKP 355
Query: 584 IKDIGNGNRAATPFALGAGHINPNRALDPGLVYDIGVQDYINLLCALNFTQKNISAITRS 643
++D + +TP+ GAGHINP +ALDPGL+YDI QDY LC + + +++
Sbjct: 356 LRDASSA-EFSTPYDHGAGHINPRKALDPGLLYDIEPQDYFEFLCTKKLSPSELVVFSKN 532
Query: 644 SFNDCS---KPSLDLNYPS 659
S +C + DLNYP+
Sbjct: 533 SNRNCKHTLASASDLNYPA 589
Score = 68.6 bits (166), Expect(2) = 8e-57
Identities = 40/91 (43%), Positives = 55/91 (59%), Gaps = 4/91 (4%)
Frame = +1
Query: 670 SRTTNEF----HRTVTNVGEKKTTYFASITPIKGFRVTVIPNKLVFKKKNEKISYKLKIE 725
S TN+F HRTVTNVG + Y TP KG V V P+ L F +K +K+SYK+ +
Sbjct: 604 SSKTNQFCFNIHRTVTNVGPAVSKYHVIGTPFKGAVVKVEPDTLNFTRKYQKLSYKISFK 783
Query: 726 GPRMTQKNKVAFGYLSWRDGKHVVRSPIVVT 756
+++++ FG L W+D H VRSPIV+T
Sbjct: 784 --VTSRQSEPEFGGLVWKDRLHKVRSPIVIT 870
>TC89137 similar to GP|4200338|emb|CAA76726.1 P69C protein {Lycopersicon
esculentum}, partial (37%)
Length = 1230
Score = 210 bits (535), Expect = 1e-54
Identities = 144/398 (36%), Positives = 209/398 (52%), Gaps = 12/398 (3%)
Frame = +3
Query: 370 YLGNFSANNFPIVFMGMCDNVKELNTVKRKIVVCEGNNETLHEQMFNVYKAKVVGGVFI- 428
Y G+ + ++ I F G K VK KIVVCE ++ K GG +
Sbjct: 6 YAGSINTSDDSIAFCGPIAMKKV--DVKGKIVVCEQGGFV--GRVAKGQAVKDAGGAAMI 173
Query: 429 ---SNILDINDVDNS--FPSIIINPVNGEIVKAYIKSHNSNASSIANMSFKKTAFGVKST 483
S D N + + P++ ++ G ++ YI NS ++ +A + FK T G +
Sbjct: 174 LLNSEGEDFNPIADVHVLPAVHVSYSAGLNIQDYI---NSTSTPMATILFKGTVIGNPNA 344
Query: 484 PSVDFYSSRGPSNSCPYVLKPDITAPGTSILAAWPTNVPVSNFGTEVFNNFNLIDGTSMS 543
P V +SSRGPS + P +LKPDI PG +ILA WP ++ S ++FN+I GTSMS
Sbjct: 345 PQVASFSSRGPSKASPGILKPDILGPGLNILAGWPISLDNST------SSFNIIAGTSMS 506
Query: 544 CPHVAGVAALLKGAHNGWSPSSIRSAIMTTSDILDNTKEHIKDIGNGNRAATPFALGAGH 603
CPH++G+AALLK +H WSP++I+SAIMTT++ ++ + I D A FA GAGH
Sbjct: 507 CPHLSGIAALLKNSHPDWSPAAIKSAIMTTANHVNLHGKPILD--QRLLPADVFATGAGH 680
Query: 604 INPNRALDPGLVYDIGVQDYINLLCALNFTQKNISAITRSSFNDCSK----PSLDLNYPS 659
+NP++A DPGLVYDI DY+ LC LN+T + I + CS P LNYPS
Sbjct: 681 VNPSKANDPGLVYDIETNDYVPYLCGLNYTDIQVGIILQQKVK-CSDVKSIPQAQLNYPS 857
Query: 660 FIAFSNARNSSRTTNEFHRTVTNVGEKKTTYFASITPIKGFRVTVIPNKLVFKKKNEKIS 719
+ T+ + RT+TNVG TTY I R++V P+++ F + +K++
Sbjct: 858 I-----SIRLGNTSQFYSRTLTNVGPVNTTYNVVIDVPVAVRMSVRPSQITFTEVKQKVT 1022
Query: 720 YKLKI--EGPRMTQKNKVAFGYLSWRDGKHVVRSPIVV 755
Y + E N +A G + W K+ V PI V
Sbjct: 1023YWVDFIPEDKENRGDNFIAQGSIKWISAKYSVSIPIAV 1136
>TC88072 similar to PIR|T06580|T06580 subtilisin-like proteinase (EC
3.4.21.-) p69f - tomato, partial (27%)
Length = 1097
Score = 206 bits (524), Expect = 3e-53
Identities = 125/319 (39%), Positives = 176/319 (54%), Gaps = 5/319 (1%)
Frame = +2
Query: 442 PSIIINPVNGEIVKAYIKSHNSNASSIANMSFKKTAFGVKSTPSVDFYSSRGPSNSCPYV 501
P++ ++ G +K+YIKS + A + FK T G PSV ++SSRGPS P +
Sbjct: 23 PAVEVSYAAGLTIKSYIKS---TYNPTATLIFKGTIIGDSLAPSVVYFSSRGPSQESPGI 193
Query: 502 LKPDITAPGTSILAAWPTNVPVSNFGTEVFNNFNLIDGTSMSCPHVAGVAALLKGAHNGW 561
LKPDI PG +ILAAW +V F+++ GTSMSCPH++G+AAL+K +H W
Sbjct: 194 LKPDIIGPGVNILAAWAVSVD------NKIPAFDIVSGTSMSCPHLSGIAALIKSSHPDW 355
Query: 562 SPSSIRSAIMTTSDILDNTKEHIKDIGNGNRAATPFALGAGHINPNRALDPGLVYDIGVQ 621
SP++I+SAIMTT++ L+ I D A FA GAGH+NP +A DPGLVYDI +
Sbjct: 356 SPAAIKSAIMTTANTLNLGGIPILD--QRLLPADIFATGAGHVNPFKANDPGLVYDIEPE 529
Query: 622 DYINLLCALNFTQKNISAITR---SSFNDCSKPSLDLNYPSFIAFSNARNSSRTTNEFHR 678
DY+ LC L ++ K I I + N S P LNYPSF + + T R
Sbjct: 530 DYVPYLCGLGYSDKEIEVIVQWKVKCSNVKSIPEAQLNYPSFSILLGSDSQYYT-----R 694
Query: 679 TVTNVGEKKTTYFASITPIKGFRVTVIPNKLVFKKKNEKISYKLKIEGPRMTQKNKVAFG 738
T+TNVG +TY + ++V P+++ F + NEK+S+ ++ + FG
Sbjct: 695 TLTNVGFANSTYKVELEVPLALGMSVNPSEITFTEVNEKVSFSVEFIPQIKENRRNQTFG 874
Query: 739 Y--LSWRDGKHVVRSPIVV 755
L+W KH VR PI V
Sbjct: 875 QGSLTWVSDKHAVRVPISV 931
>TC83163 weakly similar to GP|6721520|dbj|BAA89562.1 EST AU029428(E30359)
corresponds to a region of the predicted gene.~Similar
to antifreeze-like, partial (29%)
Length = 990
Score = 203 bits (517), Expect = 2e-52
Identities = 121/280 (43%), Positives = 162/280 (57%), Gaps = 10/280 (3%)
Frame = +2
Query: 71 IFYTYTNVMNGFSANLSPEEHESLKTFSGFISSIPDLPLKLDTTHSPQFL----GLNPYR 126
+ + Y++ NGFSA L+ E +L G +S D L+L TT S FL G+ P+
Sbjct: 209 LIHHYSHAFNGFSAMLTQSEASALAGNDGVVSVFEDPFLELHTTRSWDFLESDLGMRPH- 385
Query: 127 GAWPTSDFGKDIIVGVIDTGVWPESESFRDDGMTKIPSKWKGQLCQ---FENSNIQSINL 183
G DII+GVIDTG+WPES SF+D+G+ KIPS+WKG + F+ SN
Sbjct: 386 GILKHQHSSNDIIIGVIDTGIWPESPSFKDEGIGKIPSRWKGVCMEAHDFKKSN------ 547
Query: 184 SLCNKKLIGARFFNKGFLAKHSNISTTILNSTRDTNGHGTHTSTTAAGSKVDGASFFGYA 243
CN+KLIGAR++NK S RD NGHGTHT++TAAG V+ AS++G A
Sbjct: 548 --CNRKLIGARYYNK----------KDPKGSPRDFNGHGTHTASTAAGVIVNNASYYGLA 691
Query: 244 NGTARGIASSSRVAIYKTAWGKDGDALSSDIIAAIDAAISDGVDILSISLGSDDLLL--- 300
GTARG + S+R+A YK G+ ++ AID AI DGVDI+SIS+G L
Sbjct: 692 KGTARGGSPSARIAAYKACSGE--GCSGGTLLKAIDDAIKDGVDIISISIGFSSEFLSEY 865
Query: 301 YKDPVAIATFAAMEKGIFVSTSAGNNGPSFKSIHNGIPWV 340
DP+AI F A ++G+ V SAGN GP ++ N PW+
Sbjct: 866 LSDPIAIGAFHAEQRGVMVVCSAGNEGPDHYTVVNTTPWI 985
>TC81650 similar to PIR|T07171|T07171 subtilisin-like proteinase (EC
3.4.21.-) 1 - tomato, partial (19%)
Length = 972
Score = 197 bits (501), Expect = 1e-50
Identities = 110/263 (41%), Positives = 158/263 (59%), Gaps = 13/263 (4%)
Frame = +2
Query: 504 PDITAPGTSILAAW-----PTNVPVSNFGTEVFNNFNLIDGTSMSCPHVAGVAALLKGAH 558
PD+ APG +ILA W PT++ + E FN+I GTSMSCPHV+G+AALL+ A+
Sbjct: 2 PDVIAPGVNILAGWTGKVGPTDLEIDPRRVE----FNIISGTSMSCPHVSGIAALLRKAY 169
Query: 559 NGWSPSSIRSAIMTTSDILDNTKEHIKDIGNGNRAATPFALGAGHINPNRALDPGLVYDI 618
WSP++I+SA+MTT+ +DN+ IKD+G G + + PF GAGH++PN+AL+PGLVYD+
Sbjct: 170 PEWSPAAIKSALMTTAYNVDNSGGKIKDLGTG-KESNPFVHGAGHVDPNKALNPGLVYDL 346
Query: 619 GVQDYINLLCALNFTQKNISAITR--SSFNDCSKPSL-----DLNYPSFIAFSNARNSSR 671
+ DY+ LC++ + K I TR +S+N C DLNYPSF A N
Sbjct: 347 NINDYLAFLCSIGYDAKEIQIFTREPTSYNVCENERKFTSPGDLNYPSFSVVFGANNG-- 520
Query: 672 TTNEFHRTVTNVGEK-KTTYFASITPIKGFRVTVIPNKLVFKKKNEKISYKLKIEGPRMT 730
++ R +TNVG+ Y + G V+V P+KLVF +N+ ++++ R+
Sbjct: 521 -LVKYKRVLTNVGDSVDAVYTVKVNAPFGVDVSVSPSKLVFSSENKTQAFEVTF--TRIG 691
Query: 731 QKNKVAFGYLSWRDGKHVVRSPI 753
+FG L W DG H+VRSPI
Sbjct: 692 YGGSQSFGSLEWSDGSHIVRSPI 760
>TC89543 similar to PIR|T06580|T06580 subtilisin-like proteinase (EC
3.4.21.-) p69f - tomato, partial (14%)
Length = 1042
Score = 171 bits (434), Expect(2) = 7e-49
Identities = 103/259 (39%), Positives = 142/259 (54%), Gaps = 2/259 (0%)
Frame = +1
Query: 29 YIIHMNLSDMPKSFSNQ--HSWYESTLAQVTTTNNNLNNSTSSKIFYTYTNVMNGFSANL 86
YI+H+ S+ SF +Q HSWY S L Q N ++ ++Y +V +GF+ L
Sbjct: 145 YIVHVKKSENVASFQSQDLHSWYHSFLPQ--------NFPHKDRMVFSYRHVASGFAVKL 300
Query: 87 SPEEHESLKTFSGFISSIPDLPLKLDTTHSPQFLGLNPYRGAWPTSDFGKDIIVGVIDTG 146
+PEE +SL+ G + + P+ L L TTHSP FLGL +G W + GK +I+GVID+G
Sbjct: 301 TPEEAKSLQEKDGILLARPERTLSLHTTHSPTFLGLKHGQGLWNDDNLGKGVIIGVIDSG 480
Query: 147 VWPESESFRDDGMTKIPSKWKGQLCQFENSNIQSINLSLCNKKLIGARFFNKGFLAKHSN 206
++P SF D+GM P+KWKG C+F I CN KLIGAR S
Sbjct: 481 IYPYHPSFNDEGMPPPPAKWKGH-CEFNGRKI-------CNNKLIGAR----------SL 606
Query: 207 ISTTILNSTRDTNGHGTHTSTTAAGSKVDGASFFGYANGTARGIASSSRVAIYKTAWGKD 266
+ +TI + HGTHT+ AAG + AS FG A G G+A ++ +AIYK K
Sbjct: 607 VKSTIQEPPFENIFHGTHTAAEAAGRFIKDASVFGNAKGVXAGMAPNAHLAIYKVCNDKI 786
Query: 267 GDALSSDIIAAIDAAISDG 285
+ S I+AA+D AI DG
Sbjct: 787 -ECPESAILAAMDIAIEDG 840
Score = 41.6 bits (96), Expect(2) = 7e-49
Identities = 16/50 (32%), Positives = 28/50 (56%)
Frame = +3
Query: 302 KDPVAIATFAAMEKGIFVSTSAGNNGPSFKSIHNGIPWVITVAAGTLDRE 351
K + + ++ FV SA N+GP + ++ N PW++TV A +DR+
Sbjct: 891 KTQLQLVLLLLLKMVFFVXCSAANSGPEYSTLSNEAPWILTVGASXIDRK 1040
Score = 38.9 bits (89), Expect = 0.007
Identities = 17/33 (51%), Positives = 24/33 (72%)
Frame = +2
Query: 286 VDILSISLGSDDLLLYKDPVAIATFAAMEKGIF 318
V++LS+SLG L ++DP+AI FAA + GIF
Sbjct: 842 VNVLSLSLGLXSLPFFEDPIAIGAFAATQNGIF 940
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.317 0.133 0.394
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 23,102,444
Number of Sequences: 36976
Number of extensions: 325844
Number of successful extensions: 2269
Number of sequences better than 10.0: 105
Number of HSP's better than 10.0 without gapping: 2071
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2101
length of query: 762
length of database: 9,014,727
effective HSP length: 104
effective length of query: 658
effective length of database: 5,169,223
effective search space: 3401348734
effective search space used: 3401348734
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 62 (28.5 bits)
Medicago: description of AC144727.4