Medicago
BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= AC144727.12 - phase: 0 
         (160 letters)

Database: MTGI 
           36,976 sequences; 27,044,181 total letters

Searching..................................................done


                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

TC90270 similar to PIR|B86418|B86418 hypothetical protein AAG126...   312  3e-86
TC87163 similar to PIR|T07661|T07661 maturation protein PM3 - so...   113  4e-26
BG451854 similar to SP|P82538|TL26 Thylakoid lumenal 25.6 kDa pr...    29  0.72
TC79304 similar to GP|9759186|dbj|BAB09801.1 emb|CAB66399.1~gene...    27  4.6
TC85300 homologue to PIR|S33775|S33775 chlorophyll a/b-binding p...    26  6.1
TC85783 homologue to GP|15131688|emb|CAC48323. 2-Cys peroxiredox...    26  7.9
TC82079 weakly similar to GP|11994399|dbj|BAB02358. contains sim...    26  7.9

>TC90270 similar to PIR|B86418|B86418 hypothetical protein AAG12626.1
           [imported] - Arabidopsis thaliana, partial (97%)
          Length = 789

 Score =  312 bits (800), Expect = 3e-86
 Identities = 158/160 (98%), Positives = 158/160 (98%)
 Frame = +1

Query: 1   MANEQMKPIATLLLALNFCMYVIVISIGGWAMNRAIDHGFEIGPGFDLPAHFSPIYFPMG 60
           MANEQMKPIATLLLALNFCMYVIVISIGGWAMNRAIDHGFEI P FDLPAHFSPIYFPMG
Sbjct: 76  MANEQMKPIATLLLALNFCMYVIVISIGGWAMNRAIDHGFEISPRFDLPAHFSPIYFPMG 255

Query: 61  NAATGFFVTFALIAGVVGIGSLISGLNHIRSWTSESLPSAASVAAIAWALTVLAMGFGCK 120
           NAATGFFVTFALIAGVVGIGSLISGLNHIRSWTSESLPSAASVAAIAWALTVLAMGFGCK
Sbjct: 256 NAATGFFVTFALIAGVVGIGSLISGLNHIRSWTSESLPSAASVAAIAWALTVLAMGFGCK 435

Query: 121 EIQLNIRNSRLKTMEAFLIILTATQLFYIAAIHGAAAIRR 160
           EIQLNIRNSRLKTMEAFLIILTATQLFYIAAIHGAAAIRR
Sbjct: 436 EIQLNIRNSRLKTMEAFLIILTATQLFYIAAIHGAAAIRR 555


>TC87163 similar to PIR|T07661|T07661 maturation protein PM3 - soybean,
           partial (93%)
          Length = 865

 Score =  113 bits (282), Expect = 4e-26
 Identities = 65/142 (45%), Positives = 83/142 (57%), Gaps = 1/142 (0%)
 Frame = +1

Query: 13  LLALNFCMYVIVISIGGWAMNRAIDHGFEIGPGFDLPAHFSPIYFPMGNAATGFFVTFAL 72
           LL LN  MY IV+    W +NR I+ G    P F             GN AT FF+TF++
Sbjct: 154 LLFLNLVMYFIVLGFASWCLNRFIN-GQTYHPSFG------------GNGATMFFLTFSI 294

Query: 73  IAGVVGIGSLISGLNHIRSWTSESLPSAASVAAIAWALTVLAMGFGCKEIQL-NIRNSRL 131
           +A V+GI S   G NH+R W S+SL SA + + IAWA+T LAMG  CK+I +   R  RL
Sbjct: 295 LAAVLGIVSKFMGANHLRFWRSDSLASAGATSVIAWAVTALAMGLACKQIHIGGHRGWRL 474

Query: 132 KTMEAFLIILTATQLFYIAAIH 153
           K +EAF+IILT TQL  +   H
Sbjct: 475 KMVEAFIIILTFTQLLCLLLTH 540


>BG451854 similar to SP|P82538|TL26 Thylakoid lumenal 25.6 kDa protein
           chloroplast precursor. [Mouse-ear cress] {Arabidopsis
           thaliana}, partial (49%)
          Length = 636

 Score = 29.3 bits (64), Expect = 0.72
 Identities = 16/60 (26%), Positives = 25/60 (41%)
 Frame = -3

Query: 82  LISGLNHIRSWTSESLPSAASVAAIAWALTVLAMGFGCKEIQLNIRNSRLKTMEAFLIIL 141
           L+S    I  W +     A +  AIAW L V      C  + +    S L+ +   L++L
Sbjct: 316 LLSAAKEIEDWLNRKTNGAVAAMAIAWRLPVEEDESSCSALTMKEMPSLLRKVLLLLLLL 137


>TC79304 similar to GP|9759186|dbj|BAB09801.1
           emb|CAB66399.1~gene_id:K19E1.19~strong similarity to
           unknown protein {Arabidopsis thaliana}, partial (8%)
          Length = 790

 Score = 26.6 bits (57), Expect = 4.6
 Identities = 14/53 (26%), Positives = 23/53 (42%)
 Frame = -2

Query: 70  FALIAGVVGIGSLISGLNHIRSWTSESLPSAASVAAIAWALTVLAMGFGCKEI 122
           ++LIA    + S    L  I+ W S S+      +   W+ T+  +  GC  I
Sbjct: 321 YSLIADSSSLSSETDSLLMIKMWRSRSILIGRQCSTPRWSRTMRLVNLGCWSI 163


>TC85300 homologue to PIR|S33775|S33775 chlorophyll a/b-binding protein -
           garden pea, partial (96%)
          Length = 1163

 Score = 26.2 bits (56), Expect = 6.1
 Identities = 13/29 (44%), Positives = 16/29 (54%)
 Frame = -2

Query: 80  GSLISGLNHIRSWTSESLPSAASVAAIAW 108
           GSL S L H  S +    PSA S+A + W
Sbjct: 538 GSLKSTLTHFSSTSGVMHPSAPSIAHLPW 452


>TC85783 homologue to GP|15131688|emb|CAC48323. 2-Cys peroxiredoxin {Pisum
           sativum}, partial (91%)
          Length = 1136

 Score = 25.8 bits (55), Expect = 7.9
 Identities = 18/59 (30%), Positives = 27/59 (45%), Gaps = 3/59 (5%)
 Frame = -2

Query: 42  IGPGFDLPAHFSPIYFPMGNAATGFFVTFALIAGVVGIGSL---ISGLNHIRSWTSESL 97
           +G GF     FSP + P G  ++GF  T+     V+ + S    I  L  +  W + SL
Sbjct: 814 LGSGF---MDFSPGFHPAGQTSSGFSCTYCKAWRVLFVSSTLLPIPRLLMVECWITPSL 647


>TC82079 weakly similar to GP|11994399|dbj|BAB02358. contains similarity to
           receptor protein kinase~gene_id:MIL23.20 {Arabidopsis
           thaliana}, partial (23%)
          Length = 1057

 Score = 25.8 bits (55), Expect = 7.9
 Identities = 18/47 (38%), Positives = 23/47 (48%)
 Frame = +2

Query: 33  NRAIDHGFEIGPGFDLPAHFSPIYFPMGNAATGFFVTFALIAGVVGI 79
           N +I  G    PG D   HF P+Y   G  A G  V  A+ AG+ G+
Sbjct: 623 NFSIGSGIVFIPGRDQNGHFFPLYSRTG-IAKGSAVGIAM-AGIFGL 757


  Database: MTGI
    Posted date:  Oct 22, 2004  3:39 PM
  Number of letters in database: 27,044,181
  Number of sequences in database:  36,976
  
Lambda     K      H
   0.328    0.140    0.426 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 4,773,286
Number of Sequences: 36976
Number of extensions: 61434
Number of successful extensions: 392
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 387
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 391
length of query: 160
length of database: 9,014,727
effective HSP length: 88
effective length of query: 72
effective length of database: 5,760,839
effective search space: 414780408
effective search space used: 414780408
frameshift window, decay const: 50,  0.1
T: 13
A: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.8 bits)
S2: 54 (25.4 bits)


Medicago: description of AC144727.12