Medicago
BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= AC144725.4 + phase: 0 
         (126 letters)

Database: MTGI 
           36,976 sequences; 27,044,181 total letters

Searching..................................................done


                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

TC84217 similar to GP|13507101|gb|AAK28442.1 WRKY DNA-binding pr...    80  2e-16
TC86292 similar to GP|17064164|gb|AAL35289.1 WRKY transcription ...    73  3e-14
TC80136 similar to PIR|T50861|T50861 DNA-binding protein 4 [impo...    70  3e-13
TC86532 similar to GP|17064164|gb|AAL35289.1 WRKY transcription ...    68  8e-13
TC89911 similar to GP|13507101|gb|AAK28442.1 WRKY DNA-binding pr...    53  2e-12
BG646592 similar to GP|13507101|gb| WRKY DNA-binding protein 53 ...    52  3e-12
TC78190 weakly similar to PIR|T49041|T49041 DNA-binding protein-...    66  3e-12
TC77078 similar to GP|21537078|gb|AAM61419.1 putaive DNA-binding...    63  3e-11
TC79332 similar to PIR|JC6203|JC6203 SP8 binding protein homolog...    62  6e-11
BQ137757 similar to GP|17064164|gb| WRKY transcription factor 41...    45  3e-10
TC86084 similar to GP|21537078|gb|AAM61419.1 putaive DNA-binding...    58  8e-10
TC81471 similar to PIR|A84913|A84913 probable WRKY-type DNA bind...    57  2e-09
TC88395 similar to SP|Q9LP56|WR65_ARATH Probable WRKY transcript...    57  2e-09
TC80165 similar to PIR|A84913|A84913 probable WRKY-type DNA bind...    57  2e-09
TC89453 similar to GP|9187622|emb|CAB97004.1 WRKY DNA binding pr...    57  2e-09
TC82987 similar to GP|15991740|gb|AAL13047.1 WRKY transcription ...    56  3e-09
TC87470 similar to SP|Q9LP56|WR65_ARATH Probable WRKY transcript...    56  4e-09
TC83563 similar to PIR|T52092|T52092 DNA-binding protein WRKY2 [...    55  5e-09
TC80208 similar to GP|17064158|gb|AAL35286.1 WRKY transcription ...    54  1e-08
TC84716 homologue to GP|17064160|gb|AAL35287.1 WRKY transcriptio...    54  1e-08

>TC84217 similar to GP|13507101|gb|AAK28442.1 WRKY DNA-binding protein 53
           {Arabidopsis thaliana}, partial (21%)
          Length = 706

 Score = 80.5 bits (197), Expect = 2e-16
 Identities = 38/95 (40%), Positives = 54/95 (56%), Gaps = 2/95 (2%)
 Frame = +1

Query: 2   PPEDGFTWTKYGMKEIPGFIYPRSYYRCNHVKLYACRAKKKVQQLGDHPNIFEVRYNGGH 61
           P +DG++W KYG K+I G ++PR YYRC H  +  C A K+VQ+  + P IFE+ Y G H
Sbjct: 427 PLDDGYSWRKYGQKDILGAMHPRGYYRCTHRNVLGCLATKQVQRSDEDPTIFEITYRGNH 606

Query: 62  TCRMSLTI--PSLFVPARQPLDISIDGIQSTMPTS 94
           TC ++  +  P+L      P       I +T P S
Sbjct: 607 TCTIASNVVGPTL----NNPFQTQETNIVNTNPQS 699


>TC86292 similar to GP|17064164|gb|AAL35289.1 WRKY transcription factor 41
           {Arabidopsis thaliana}, partial (21%)
          Length = 1529

 Score = 72.8 bits (177), Expect = 3e-14
 Identities = 31/62 (50%), Positives = 39/62 (62%)
 Frame = +3

Query: 2   PPEDGFTWTKYGMKEIPGFIYPRSYYRCNHVKLYACRAKKKVQQLGDHPNIFEVRYNGGH 61
           P EDG+ W KYG K+I G  +PRSYYRC       C A K+VQ+  + P IF++ Y G H
Sbjct: 609 PNEDGYNWRKYGQKDILGTKHPRSYYRCTFRNTQDCWATKQVQRSDEDPTIFDITYRGKH 788

Query: 62  TC 63
           TC
Sbjct: 789 TC 794


>TC80136 similar to PIR|T50861|T50861 DNA-binding protein 4 [imported] -
           common tobacco (fragment), partial (33%)
          Length = 1276

 Score = 69.7 bits (169), Expect = 3e-13
 Identities = 32/73 (43%), Positives = 44/73 (59%)
 Frame = +2

Query: 2   PPEDGFTWTKYGMKEIPGFIYPRSYYRCNHVKLYACRAKKKVQQLGDHPNIFEVRYNGGH 61
           P EDG  W KYG K+I    +PR+YYRC H     C+A K+VQ++ + P +    Y+G H
Sbjct: 425 PEEDGHQWRKYGQKKILHTDFPRNYYRCTHKNDQGCKAIKQVQRIQEDPPLHRTTYSGHH 604

Query: 62  TCRMSLTIPSLFV 74
           TCR+ L  P + V
Sbjct: 605 TCRI-LQNPEIIV 640


>TC86532 similar to GP|17064164|gb|AAL35289.1 WRKY transcription factor 41
           {Arabidopsis thaliana}, partial (25%)
          Length = 1620

 Score = 68.2 bits (165), Expect = 8e-13
 Identities = 30/63 (47%), Positives = 37/63 (58%)
 Frame = +1

Query: 4   EDGFTWTKYGMKEIPGFIYPRSYYRCNHVKLYACRAKKKVQQLGDHPNIFEVRYNGGHTC 63
           EDG +W KYG K+I G  +PR YYRC H     C A K+VQ+  +   I E+ Y G HTC
Sbjct: 637 EDGHSWRKYGQKDILGAKFPRGYYRCTHRHAQGCLATKQVQKSDEDEMICEITYKGRHTC 816

Query: 64  RMS 66
             S
Sbjct: 817 TQS 825


>TC89911 similar to GP|13507101|gb|AAK28442.1 WRKY DNA-binding protein 53
           {Arabidopsis thaliana}, partial (21%)
          Length = 1302

 Score = 52.8 bits (125), Expect(2) = 2e-12
 Identities = 27/73 (36%), Positives = 37/73 (49%), Gaps = 2/73 (2%)
 Frame = +3

Query: 24  RSYYRCNHVKLYACRAKKKVQQLGDHPNIFEVRYNGGHTCRMSLTI--PSLFVPARQPLD 81
           R YYRC H  +  C A K+VQ+  + P IFE+ Y G HTC ++  +  P+L      P  
Sbjct: 540 RGYYRCTHRNVLGCLATKQVQRSDEDPTIFEITYRGNHTCTIASNVVGPTL----NNPFQ 707

Query: 82  ISIDGIQSTMPTS 94
                I +T P S
Sbjct: 708 TQETNIVNTNPQS 746



 Score = 34.3 bits (77), Expect(2) = 2e-12
 Identities = 12/23 (52%), Positives = 18/23 (78%)
 Frame = +1

Query: 2   PPEDGFTWTKYGMKEIPGFIYPR 24
           P +DG++W KYG K+I G ++PR
Sbjct: 397 PLDDGYSWRKYGQKDILGAMHPR 465


>BG646592 similar to GP|13507101|gb| WRKY DNA-binding protein 53 {Arabidopsis
           thaliana}, partial (19%)
          Length = 638

 Score = 52.0 bits (123), Expect(2) = 3e-12
 Identities = 20/46 (43%), Positives = 28/46 (60%)
 Frame = +1

Query: 24  RSYYRCNHVKLYACRAKKKVQQLGDHPNIFEVRYNGGHTCRMSLTI 69
           R YYRC H  +  C A K+VQ+  + P IFE+ Y G HTC ++  +
Sbjct: 421 RGYYRCTHRNVLGCLATKQVQRSDEDPTIFEITYRGNHTCTIASNV 558



 Score = 34.3 bits (77), Expect(2) = 3e-12
 Identities = 12/23 (52%), Positives = 18/23 (78%)
 Frame = +2

Query: 2   PPEDGFTWTKYGMKEIPGFIYPR 24
           P +DG++W KYG K+I G ++PR
Sbjct: 278 PLDDGYSWRKYGQKDILGAMHPR 346


>TC78190 weakly similar to PIR|T49041|T49041 DNA-binding protein-like -
           Arabidopsis thaliana, partial (27%)
          Length = 1254

 Score = 66.2 bits (160), Expect = 3e-12
 Identities = 36/116 (31%), Positives = 61/116 (52%)
 Frame = +1

Query: 5   DGFTWTKYGMKEIPGFIYPRSYYRCNHVKLYACRAKKKVQQLGDHPNIFEVRYNGGHTCR 64
           D  +W KYG KEI    +PRSY+RC       C+A K+VQ + ++P ++++ Y G HTC+
Sbjct: 532 DTHSWRKYGQKEILNSNFPRSYFRCTRKHDQGCKATKQVQLIEENPEMYQITYIGFHTCK 711

Query: 65  MSLTIPSLFVPARQPLDISIDGIQSTMPTSSTLYSRRISSGMPGIFLLPINNERSV 120
            +L  P       Q +  S D    ++  +S  +S+ + +         I++ERS+
Sbjct: 712 STLHTP-------QMVSFSEDTNWDSILVNSNPHSKEVLTNYDQQDSHVISSERSI 858


>TC77078 similar to GP|21537078|gb|AAM61419.1 putaive DNA-binding protein
           {Arabidopsis thaliana}, partial (51%)
          Length = 1346

 Score = 62.8 bits (151), Expect = 3e-11
 Identities = 32/78 (41%), Positives = 43/78 (55%)
 Frame = +2

Query: 3   PEDGFTWTKYGMKEIPGFIYPRSYYRCNHVKLYACRAKKKVQQLGDHPNIFEVRYNGGHT 62
           P D F+W KYG K I G  YPR YY+C+  K   C A+K V++  D PN+  V Y G H 
Sbjct: 842 PSDEFSWRKYGQKPIKGSPYPRGYYKCSSFK--GCPARKHVERAQDDPNMLVVTYEGEHR 1015

Query: 63  CRMSLTIPSLFVPARQPL 80
              ++   + F+   QPL
Sbjct: 1016HAQTVVTGAGFI--SQPL 1063


>TC79332 similar to PIR|JC6203|JC6203 SP8 binding protein homolog -
           cucumber, partial (49%)
          Length = 1486

 Score = 62.0 bits (149), Expect = 6e-11
 Identities = 28/60 (46%), Positives = 38/60 (62%)
 Frame = +2

Query: 2   PPEDGFTWTKYGMKEIPGFIYPRSYYRCNHVKLYACRAKKKVQQLGDHPNIFEVRYNGGH 61
           P +DG+ W KYG K++ G  YPRSYY+C H+    C  KKKV++  D  +I E+ Y G H
Sbjct: 719 PADDGYNWRKYGQKQVKGSEYPRSYYKCTHLN---CPVKKKVERAPD-GHITEIIYKGQH 886



 Score = 26.9 bits (58), Expect = 2.1
 Identities = 9/20 (45%), Positives = 13/20 (65%)
 Frame = +3

Query: 4    EDGFTWTKYGMKEIPGFIYP 23
            +DG+ W KYG K + G  +P
Sbjct: 1272 DDGYRWRKYGQKVVKGNPHP 1331


>BQ137757 similar to GP|17064164|gb| WRKY transcription factor 41
           {Arabidopsis thaliana}, partial (21%)
          Length = 721

 Score = 45.4 bits (106), Expect(2) = 3e-10
 Identities = 19/40 (47%), Positives = 24/40 (59%)
 Frame = +1

Query: 24  RSYYRCNHVKLYACRAKKKVQQLGDHPNIFEVRYNGGHTC 63
           RSYYRC       C A K+VQ+  + P IF++ Y G HTC
Sbjct: 232 RSYYRCTFRNTQDCWATKQVQRSDEDPTIFDITYRGKHTC 351



 Score = 33.9 bits (76), Expect(2) = 3e-10
 Identities = 13/23 (56%), Positives = 16/23 (69%)
 Frame = +3

Query: 2  PPEDGFTWTKYGMKEIPGFIYPR 24
          P EDG+ W KYG K+I G  +PR
Sbjct: 51 PNEDGYNWRKYGQKDILGTKHPR 119


>TC86084 similar to GP|21537078|gb|AAM61419.1 putaive DNA-binding protein
           {Arabidopsis thaliana}, partial (56%)
          Length = 1372

 Score = 58.2 bits (139), Expect = 8e-10
 Identities = 26/59 (44%), Positives = 35/59 (59%)
 Frame = +3

Query: 3   PEDGFTWTKYGMKEIPGFIYPRSYYRCNHVKLYACRAKKKVQQLGDHPNIFEVRYNGGH 61
           P D ++W KYG K I G  YPR YY+C+ V+   C A+K V++  D P +  V Y G H
Sbjct: 867 PPDEYSWRKYGQKPIKGSPYPRGYYKCSTVR--GCPARKHVERATDDPTMLIVTYEGEH 1037


>TC81471 similar to PIR|A84913|A84913 probable WRKY-type DNA binding protein
           [imported] - Arabidopsis thaliana, partial (43%)
          Length = 1274

 Score = 57.0 bits (136), Expect = 2e-09
 Identities = 32/89 (35%), Positives = 42/89 (46%), Gaps = 2/89 (2%)
 Frame = +2

Query: 4   EDGFTWTKYGMKEIPGFIYPRSYYRCNHVKLYACRAKKKVQQLGDHPNIFEVRYNGGHTC 63
           EDG+ W KYG K +    +PRSYYRC      +C  KK+V++    P+I    Y G HT 
Sbjct: 557 EDGYRWRKYGQKAVKNSPFPRSYYRCTTA---SCNVKKRVERSYTDPSIVVTTYEGQHTH 727

Query: 64  RMSLTIPSLF--VPARQPLDISIDGIQST 90
                  S F  V   QP  +   G  +T
Sbjct: 728 PSPTMSRSAFAGVQIPQPAGVVSGGFSTT 814


>TC88395 similar to SP|Q9LP56|WR65_ARATH Probable WRKY transcription factor
           65 (WRKY DNA-binding protein 65). [Mouse-ear cress],
           partial (43%)
          Length = 1086

 Score = 57.0 bits (136), Expect = 2e-09
 Identities = 25/60 (41%), Positives = 35/60 (57%)
 Frame = +2

Query: 2   PPEDGFTWTKYGMKEIPGFIYPRSYYRCNHVKLYACRAKKKVQQLGDHPNIFEVRYNGGH 61
           PP D + W KYG K I G  YPR+YYRC+  K   C A+K+V++    P +  + Y+  H
Sbjct: 245 PPSDSWAWRKYGQKPIKGSPYPRAYYRCSSCK--GCPARKQVERSRVDPTMLIITYSSDH 418


>TC80165 similar to PIR|A84913|A84913 probable WRKY-type DNA binding protein
           [imported] - Arabidopsis thaliana, partial (32%)
          Length = 1013

 Score = 56.6 bits (135), Expect = 2e-09
 Identities = 26/61 (42%), Positives = 33/61 (53%)
 Frame = +1

Query: 4   EDGFTWTKYGMKEIPGFIYPRSYYRCNHVKLYACRAKKKVQQLGDHPNIFEVRYNGGHTC 63
           EDG+ W KYG K +    +PRSYYRC  V   +C  KK V++    P I    Y G HT 
Sbjct: 634 EDGYRWRKYGQKAVKNSPFPRSYYRCTSV---SCNVKKHVERSLSDPTIVVTTYEGKHTS 804

Query: 64  R 64
           +
Sbjct: 805 K 807


>TC89453 similar to GP|9187622|emb|CAB97004.1 WRKY DNA binding protein
           {Solanum tuberosum}, partial (56%)
          Length = 701

 Score = 56.6 bits (135), Expect = 2e-09
 Identities = 25/59 (42%), Positives = 32/59 (53%)
 Frame = +1

Query: 4   EDGFTWTKYGMKEIPGFIYPRSYYRCNHVKLYACRAKKKVQQLGDHPNIFEVRYNGGHT 62
           +DG+ W KYG K +    +PRSYYRC H     C  KK+VQ+L     +    Y G HT
Sbjct: 463 DDGYRWRKYGQKAVKNNKFPRSYYRCTH---QGCNVKKQVQRLTKDEGVVVTTYEGVHT 630


>TC82987 similar to GP|15991740|gb|AAL13047.1 WRKY transcription factor 71
           {Arabidopsis thaliana}, partial (33%)
          Length = 975

 Score = 56.2 bits (134), Expect = 3e-09
 Identities = 27/66 (40%), Positives = 34/66 (50%)
 Frame = +1

Query: 4   EDGFTWTKYGMKEIPGFIYPRSYYRCNHVKLYACRAKKKVQQLGDHPNIFEVRYNGGHTC 63
           EDG+ W KYG K +    YPRSYYRC   K   C  KK+V++    P+I    Y G H  
Sbjct: 679 EDGYRWRKYGQKAVKNSPYPRSYYRCTSQK---CIVKKRVERSYQDPSIVMTTYEGQHNH 849

Query: 64  RMSLTI 69
               T+
Sbjct: 850 HCPATL 867


>TC87470 similar to SP|Q9LP56|WR65_ARATH Probable WRKY transcription factor
           65 (WRKY DNA-binding protein 65). [Mouse-ear cress],
           partial (31%)
          Length = 1057

 Score = 55.8 bits (133), Expect = 4e-09
 Identities = 26/60 (43%), Positives = 33/60 (54%)
 Frame = +3

Query: 2   PPEDGFTWTKYGMKEIPGFIYPRSYYRCNHVKLYACRAKKKVQQLGDHPNIFEVRYNGGH 61
           PP D + W KYG K I G  YPR YYRC+  K   C A+K+V++    P +  V Y   H
Sbjct: 306 PPSDSWAWRKYGQKPIKGSPYPRGYYRCSSSK--NCPARKQVERNHMDPTVLLVTYTSDH 479


>TC83563 similar to PIR|T52092|T52092 DNA-binding protein WRKY2 [imported] -
           common tobacco, partial (21%)
          Length = 838

 Score = 55.5 bits (132), Expect = 5e-09
 Identities = 27/61 (44%), Positives = 36/61 (58%), Gaps = 1/61 (1%)
 Frame = +1

Query: 2   PPEDGFTWTKYGMKEIPGFIYPRSYYRCNHVKLYACRAKKKVQQ-LGDHPNIFEVRYNGG 60
           P +DG+ W KYG K++ G  +PRSYY+C H     C  KKKV++ L  H  I  + Y G 
Sbjct: 640 PADDGYNWRKYGQKQVKGSEFPRSYYKCTHPN---CPVKKKVERSLAGH--ITAIIYKGE 804

Query: 61  H 61
           H
Sbjct: 805 H 807


>TC80208 similar to GP|17064158|gb|AAL35286.1 WRKY transcription factor 28
           {Arabidopsis thaliana}, partial (31%)
          Length = 1294

 Score = 54.3 bits (129), Expect = 1e-08
 Identities = 25/58 (43%), Positives = 31/58 (53%)
 Frame = +1

Query: 4   EDGFTWTKYGMKEIPGFIYPRSYYRCNHVKLYACRAKKKVQQLGDHPNIFEVRYNGGH 61
           EDG+ W KYG K +    YPRSYYRC   K   C  KK+V++  + P      Y G H
Sbjct: 607 EDGYRWRKYGQKAVKNSPYPRSYYRCTTQK---CTVKKRVERSYEDPTTVITTYEGQH 771


>TC84716 homologue to GP|17064160|gb|AAL35287.1 WRKY transcription factor 29
           {Arabidopsis thaliana}, partial (21%)
          Length = 524

 Score = 54.3 bits (129), Expect = 1e-08
 Identities = 26/57 (45%), Positives = 33/57 (57%)
 Frame = +2

Query: 5   DGFTWTKYGMKEIPGFIYPRSYYRCNHVKLYACRAKKKVQQLGDHPNIFEVRYNGGH 61
           D + W KYG K I G  YPRSYYRC+  K   C AKK+V++    P ++ V Y   H
Sbjct: 356 DAWAWRKYGQKPIKGSPYPRSYYRCSSSK--GCLAKKQVEKNHLDPKVYLVTYTAEH 520


  Database: MTGI
    Posted date:  Oct 22, 2004  3:39 PM
  Number of letters in database: 27,044,181
  Number of sequences in database:  36,976
  
Lambda     K      H
   0.322    0.139    0.429 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 4,504,004
Number of Sequences: 36976
Number of extensions: 64128
Number of successful extensions: 312
Number of sequences better than 10.0: 87
Number of HSP's better than 10.0 without gapping: 303
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 306
length of query: 126
length of database: 9,014,727
effective HSP length: 85
effective length of query: 41
effective length of database: 5,871,767
effective search space: 240742447
effective search space used: 240742447
frameshift window, decay const: 50,  0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 52 (24.6 bits)


Medicago: description of AC144725.4