
BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC144725.4 + phase: 0
(126 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC84217 similar to GP|13507101|gb|AAK28442.1 WRKY DNA-binding pr... 80 2e-16
TC86292 similar to GP|17064164|gb|AAL35289.1 WRKY transcription ... 73 3e-14
TC80136 similar to PIR|T50861|T50861 DNA-binding protein 4 [impo... 70 3e-13
TC86532 similar to GP|17064164|gb|AAL35289.1 WRKY transcription ... 68 8e-13
TC89911 similar to GP|13507101|gb|AAK28442.1 WRKY DNA-binding pr... 53 2e-12
BG646592 similar to GP|13507101|gb| WRKY DNA-binding protein 53 ... 52 3e-12
TC78190 weakly similar to PIR|T49041|T49041 DNA-binding protein-... 66 3e-12
TC77078 similar to GP|21537078|gb|AAM61419.1 putaive DNA-binding... 63 3e-11
TC79332 similar to PIR|JC6203|JC6203 SP8 binding protein homolog... 62 6e-11
BQ137757 similar to GP|17064164|gb| WRKY transcription factor 41... 45 3e-10
TC86084 similar to GP|21537078|gb|AAM61419.1 putaive DNA-binding... 58 8e-10
TC81471 similar to PIR|A84913|A84913 probable WRKY-type DNA bind... 57 2e-09
TC88395 similar to SP|Q9LP56|WR65_ARATH Probable WRKY transcript... 57 2e-09
TC80165 similar to PIR|A84913|A84913 probable WRKY-type DNA bind... 57 2e-09
TC89453 similar to GP|9187622|emb|CAB97004.1 WRKY DNA binding pr... 57 2e-09
TC82987 similar to GP|15991740|gb|AAL13047.1 WRKY transcription ... 56 3e-09
TC87470 similar to SP|Q9LP56|WR65_ARATH Probable WRKY transcript... 56 4e-09
TC83563 similar to PIR|T52092|T52092 DNA-binding protein WRKY2 [... 55 5e-09
TC80208 similar to GP|17064158|gb|AAL35286.1 WRKY transcription ... 54 1e-08
TC84716 homologue to GP|17064160|gb|AAL35287.1 WRKY transcriptio... 54 1e-08
>TC84217 similar to GP|13507101|gb|AAK28442.1 WRKY DNA-binding protein 53
{Arabidopsis thaliana}, partial (21%)
Length = 706
Score = 80.5 bits (197), Expect = 2e-16
Identities = 38/95 (40%), Positives = 54/95 (56%), Gaps = 2/95 (2%)
Frame = +1
Query: 2 PPEDGFTWTKYGMKEIPGFIYPRSYYRCNHVKLYACRAKKKVQQLGDHPNIFEVRYNGGH 61
P +DG++W KYG K+I G ++PR YYRC H + C A K+VQ+ + P IFE+ Y G H
Sbjct: 427 PLDDGYSWRKYGQKDILGAMHPRGYYRCTHRNVLGCLATKQVQRSDEDPTIFEITYRGNH 606
Query: 62 TCRMSLTI--PSLFVPARQPLDISIDGIQSTMPTS 94
TC ++ + P+L P I +T P S
Sbjct: 607 TCTIASNVVGPTL----NNPFQTQETNIVNTNPQS 699
>TC86292 similar to GP|17064164|gb|AAL35289.1 WRKY transcription factor 41
{Arabidopsis thaliana}, partial (21%)
Length = 1529
Score = 72.8 bits (177), Expect = 3e-14
Identities = 31/62 (50%), Positives = 39/62 (62%)
Frame = +3
Query: 2 PPEDGFTWTKYGMKEIPGFIYPRSYYRCNHVKLYACRAKKKVQQLGDHPNIFEVRYNGGH 61
P EDG+ W KYG K+I G +PRSYYRC C A K+VQ+ + P IF++ Y G H
Sbjct: 609 PNEDGYNWRKYGQKDILGTKHPRSYYRCTFRNTQDCWATKQVQRSDEDPTIFDITYRGKH 788
Query: 62 TC 63
TC
Sbjct: 789 TC 794
>TC80136 similar to PIR|T50861|T50861 DNA-binding protein 4 [imported] -
common tobacco (fragment), partial (33%)
Length = 1276
Score = 69.7 bits (169), Expect = 3e-13
Identities = 32/73 (43%), Positives = 44/73 (59%)
Frame = +2
Query: 2 PPEDGFTWTKYGMKEIPGFIYPRSYYRCNHVKLYACRAKKKVQQLGDHPNIFEVRYNGGH 61
P EDG W KYG K+I +PR+YYRC H C+A K+VQ++ + P + Y+G H
Sbjct: 425 PEEDGHQWRKYGQKKILHTDFPRNYYRCTHKNDQGCKAIKQVQRIQEDPPLHRTTYSGHH 604
Query: 62 TCRMSLTIPSLFV 74
TCR+ L P + V
Sbjct: 605 TCRI-LQNPEIIV 640
>TC86532 similar to GP|17064164|gb|AAL35289.1 WRKY transcription factor 41
{Arabidopsis thaliana}, partial (25%)
Length = 1620
Score = 68.2 bits (165), Expect = 8e-13
Identities = 30/63 (47%), Positives = 37/63 (58%)
Frame = +1
Query: 4 EDGFTWTKYGMKEIPGFIYPRSYYRCNHVKLYACRAKKKVQQLGDHPNIFEVRYNGGHTC 63
EDG +W KYG K+I G +PR YYRC H C A K+VQ+ + I E+ Y G HTC
Sbjct: 637 EDGHSWRKYGQKDILGAKFPRGYYRCTHRHAQGCLATKQVQKSDEDEMICEITYKGRHTC 816
Query: 64 RMS 66
S
Sbjct: 817 TQS 825
>TC89911 similar to GP|13507101|gb|AAK28442.1 WRKY DNA-binding protein 53
{Arabidopsis thaliana}, partial (21%)
Length = 1302
Score = 52.8 bits (125), Expect(2) = 2e-12
Identities = 27/73 (36%), Positives = 37/73 (49%), Gaps = 2/73 (2%)
Frame = +3
Query: 24 RSYYRCNHVKLYACRAKKKVQQLGDHPNIFEVRYNGGHTCRMSLTI--PSLFVPARQPLD 81
R YYRC H + C A K+VQ+ + P IFE+ Y G HTC ++ + P+L P
Sbjct: 540 RGYYRCTHRNVLGCLATKQVQRSDEDPTIFEITYRGNHTCTIASNVVGPTL----NNPFQ 707
Query: 82 ISIDGIQSTMPTS 94
I +T P S
Sbjct: 708 TQETNIVNTNPQS 746
Score = 34.3 bits (77), Expect(2) = 2e-12
Identities = 12/23 (52%), Positives = 18/23 (78%)
Frame = +1
Query: 2 PPEDGFTWTKYGMKEIPGFIYPR 24
P +DG++W KYG K+I G ++PR
Sbjct: 397 PLDDGYSWRKYGQKDILGAMHPR 465
>BG646592 similar to GP|13507101|gb| WRKY DNA-binding protein 53 {Arabidopsis
thaliana}, partial (19%)
Length = 638
Score = 52.0 bits (123), Expect(2) = 3e-12
Identities = 20/46 (43%), Positives = 28/46 (60%)
Frame = +1
Query: 24 RSYYRCNHVKLYACRAKKKVQQLGDHPNIFEVRYNGGHTCRMSLTI 69
R YYRC H + C A K+VQ+ + P IFE+ Y G HTC ++ +
Sbjct: 421 RGYYRCTHRNVLGCLATKQVQRSDEDPTIFEITYRGNHTCTIASNV 558
Score = 34.3 bits (77), Expect(2) = 3e-12
Identities = 12/23 (52%), Positives = 18/23 (78%)
Frame = +2
Query: 2 PPEDGFTWTKYGMKEIPGFIYPR 24
P +DG++W KYG K+I G ++PR
Sbjct: 278 PLDDGYSWRKYGQKDILGAMHPR 346
>TC78190 weakly similar to PIR|T49041|T49041 DNA-binding protein-like -
Arabidopsis thaliana, partial (27%)
Length = 1254
Score = 66.2 bits (160), Expect = 3e-12
Identities = 36/116 (31%), Positives = 61/116 (52%)
Frame = +1
Query: 5 DGFTWTKYGMKEIPGFIYPRSYYRCNHVKLYACRAKKKVQQLGDHPNIFEVRYNGGHTCR 64
D +W KYG KEI +PRSY+RC C+A K+VQ + ++P ++++ Y G HTC+
Sbjct: 532 DTHSWRKYGQKEILNSNFPRSYFRCTRKHDQGCKATKQVQLIEENPEMYQITYIGFHTCK 711
Query: 65 MSLTIPSLFVPARQPLDISIDGIQSTMPTSSTLYSRRISSGMPGIFLLPINNERSV 120
+L P Q + S D ++ +S +S+ + + I++ERS+
Sbjct: 712 STLHTP-------QMVSFSEDTNWDSILVNSNPHSKEVLTNYDQQDSHVISSERSI 858
>TC77078 similar to GP|21537078|gb|AAM61419.1 putaive DNA-binding protein
{Arabidopsis thaliana}, partial (51%)
Length = 1346
Score = 62.8 bits (151), Expect = 3e-11
Identities = 32/78 (41%), Positives = 43/78 (55%)
Frame = +2
Query: 3 PEDGFTWTKYGMKEIPGFIYPRSYYRCNHVKLYACRAKKKVQQLGDHPNIFEVRYNGGHT 62
P D F+W KYG K I G YPR YY+C+ K C A+K V++ D PN+ V Y G H
Sbjct: 842 PSDEFSWRKYGQKPIKGSPYPRGYYKCSSFK--GCPARKHVERAQDDPNMLVVTYEGEHR 1015
Query: 63 CRMSLTIPSLFVPARQPL 80
++ + F+ QPL
Sbjct: 1016HAQTVVTGAGFI--SQPL 1063
>TC79332 similar to PIR|JC6203|JC6203 SP8 binding protein homolog -
cucumber, partial (49%)
Length = 1486
Score = 62.0 bits (149), Expect = 6e-11
Identities = 28/60 (46%), Positives = 38/60 (62%)
Frame = +2
Query: 2 PPEDGFTWTKYGMKEIPGFIYPRSYYRCNHVKLYACRAKKKVQQLGDHPNIFEVRYNGGH 61
P +DG+ W KYG K++ G YPRSYY+C H+ C KKKV++ D +I E+ Y G H
Sbjct: 719 PADDGYNWRKYGQKQVKGSEYPRSYYKCTHLN---CPVKKKVERAPD-GHITEIIYKGQH 886
Score = 26.9 bits (58), Expect = 2.1
Identities = 9/20 (45%), Positives = 13/20 (65%)
Frame = +3
Query: 4 EDGFTWTKYGMKEIPGFIYP 23
+DG+ W KYG K + G +P
Sbjct: 1272 DDGYRWRKYGQKVVKGNPHP 1331
>BQ137757 similar to GP|17064164|gb| WRKY transcription factor 41
{Arabidopsis thaliana}, partial (21%)
Length = 721
Score = 45.4 bits (106), Expect(2) = 3e-10
Identities = 19/40 (47%), Positives = 24/40 (59%)
Frame = +1
Query: 24 RSYYRCNHVKLYACRAKKKVQQLGDHPNIFEVRYNGGHTC 63
RSYYRC C A K+VQ+ + P IF++ Y G HTC
Sbjct: 232 RSYYRCTFRNTQDCWATKQVQRSDEDPTIFDITYRGKHTC 351
Score = 33.9 bits (76), Expect(2) = 3e-10
Identities = 13/23 (56%), Positives = 16/23 (69%)
Frame = +3
Query: 2 PPEDGFTWTKYGMKEIPGFIYPR 24
P EDG+ W KYG K+I G +PR
Sbjct: 51 PNEDGYNWRKYGQKDILGTKHPR 119
>TC86084 similar to GP|21537078|gb|AAM61419.1 putaive DNA-binding protein
{Arabidopsis thaliana}, partial (56%)
Length = 1372
Score = 58.2 bits (139), Expect = 8e-10
Identities = 26/59 (44%), Positives = 35/59 (59%)
Frame = +3
Query: 3 PEDGFTWTKYGMKEIPGFIYPRSYYRCNHVKLYACRAKKKVQQLGDHPNIFEVRYNGGH 61
P D ++W KYG K I G YPR YY+C+ V+ C A+K V++ D P + V Y G H
Sbjct: 867 PPDEYSWRKYGQKPIKGSPYPRGYYKCSTVR--GCPARKHVERATDDPTMLIVTYEGEH 1037
>TC81471 similar to PIR|A84913|A84913 probable WRKY-type DNA binding protein
[imported] - Arabidopsis thaliana, partial (43%)
Length = 1274
Score = 57.0 bits (136), Expect = 2e-09
Identities = 32/89 (35%), Positives = 42/89 (46%), Gaps = 2/89 (2%)
Frame = +2
Query: 4 EDGFTWTKYGMKEIPGFIYPRSYYRCNHVKLYACRAKKKVQQLGDHPNIFEVRYNGGHTC 63
EDG+ W KYG K + +PRSYYRC +C KK+V++ P+I Y G HT
Sbjct: 557 EDGYRWRKYGQKAVKNSPFPRSYYRCTTA---SCNVKKRVERSYTDPSIVVTTYEGQHTH 727
Query: 64 RMSLTIPSLF--VPARQPLDISIDGIQST 90
S F V QP + G +T
Sbjct: 728 PSPTMSRSAFAGVQIPQPAGVVSGGFSTT 814
>TC88395 similar to SP|Q9LP56|WR65_ARATH Probable WRKY transcription factor
65 (WRKY DNA-binding protein 65). [Mouse-ear cress],
partial (43%)
Length = 1086
Score = 57.0 bits (136), Expect = 2e-09
Identities = 25/60 (41%), Positives = 35/60 (57%)
Frame = +2
Query: 2 PPEDGFTWTKYGMKEIPGFIYPRSYYRCNHVKLYACRAKKKVQQLGDHPNIFEVRYNGGH 61
PP D + W KYG K I G YPR+YYRC+ K C A+K+V++ P + + Y+ H
Sbjct: 245 PPSDSWAWRKYGQKPIKGSPYPRAYYRCSSCK--GCPARKQVERSRVDPTMLIITYSSDH 418
>TC80165 similar to PIR|A84913|A84913 probable WRKY-type DNA binding protein
[imported] - Arabidopsis thaliana, partial (32%)
Length = 1013
Score = 56.6 bits (135), Expect = 2e-09
Identities = 26/61 (42%), Positives = 33/61 (53%)
Frame = +1
Query: 4 EDGFTWTKYGMKEIPGFIYPRSYYRCNHVKLYACRAKKKVQQLGDHPNIFEVRYNGGHTC 63
EDG+ W KYG K + +PRSYYRC V +C KK V++ P I Y G HT
Sbjct: 634 EDGYRWRKYGQKAVKNSPFPRSYYRCTSV---SCNVKKHVERSLSDPTIVVTTYEGKHTS 804
Query: 64 R 64
+
Sbjct: 805 K 807
>TC89453 similar to GP|9187622|emb|CAB97004.1 WRKY DNA binding protein
{Solanum tuberosum}, partial (56%)
Length = 701
Score = 56.6 bits (135), Expect = 2e-09
Identities = 25/59 (42%), Positives = 32/59 (53%)
Frame = +1
Query: 4 EDGFTWTKYGMKEIPGFIYPRSYYRCNHVKLYACRAKKKVQQLGDHPNIFEVRYNGGHT 62
+DG+ W KYG K + +PRSYYRC H C KK+VQ+L + Y G HT
Sbjct: 463 DDGYRWRKYGQKAVKNNKFPRSYYRCTH---QGCNVKKQVQRLTKDEGVVVTTYEGVHT 630
>TC82987 similar to GP|15991740|gb|AAL13047.1 WRKY transcription factor 71
{Arabidopsis thaliana}, partial (33%)
Length = 975
Score = 56.2 bits (134), Expect = 3e-09
Identities = 27/66 (40%), Positives = 34/66 (50%)
Frame = +1
Query: 4 EDGFTWTKYGMKEIPGFIYPRSYYRCNHVKLYACRAKKKVQQLGDHPNIFEVRYNGGHTC 63
EDG+ W KYG K + YPRSYYRC K C KK+V++ P+I Y G H
Sbjct: 679 EDGYRWRKYGQKAVKNSPYPRSYYRCTSQK---CIVKKRVERSYQDPSIVMTTYEGQHNH 849
Query: 64 RMSLTI 69
T+
Sbjct: 850 HCPATL 867
>TC87470 similar to SP|Q9LP56|WR65_ARATH Probable WRKY transcription factor
65 (WRKY DNA-binding protein 65). [Mouse-ear cress],
partial (31%)
Length = 1057
Score = 55.8 bits (133), Expect = 4e-09
Identities = 26/60 (43%), Positives = 33/60 (54%)
Frame = +3
Query: 2 PPEDGFTWTKYGMKEIPGFIYPRSYYRCNHVKLYACRAKKKVQQLGDHPNIFEVRYNGGH 61
PP D + W KYG K I G YPR YYRC+ K C A+K+V++ P + V Y H
Sbjct: 306 PPSDSWAWRKYGQKPIKGSPYPRGYYRCSSSK--NCPARKQVERNHMDPTVLLVTYTSDH 479
>TC83563 similar to PIR|T52092|T52092 DNA-binding protein WRKY2 [imported] -
common tobacco, partial (21%)
Length = 838
Score = 55.5 bits (132), Expect = 5e-09
Identities = 27/61 (44%), Positives = 36/61 (58%), Gaps = 1/61 (1%)
Frame = +1
Query: 2 PPEDGFTWTKYGMKEIPGFIYPRSYYRCNHVKLYACRAKKKVQQ-LGDHPNIFEVRYNGG 60
P +DG+ W KYG K++ G +PRSYY+C H C KKKV++ L H I + Y G
Sbjct: 640 PADDGYNWRKYGQKQVKGSEFPRSYYKCTHPN---CPVKKKVERSLAGH--ITAIIYKGE 804
Query: 61 H 61
H
Sbjct: 805 H 807
>TC80208 similar to GP|17064158|gb|AAL35286.1 WRKY transcription factor 28
{Arabidopsis thaliana}, partial (31%)
Length = 1294
Score = 54.3 bits (129), Expect = 1e-08
Identities = 25/58 (43%), Positives = 31/58 (53%)
Frame = +1
Query: 4 EDGFTWTKYGMKEIPGFIYPRSYYRCNHVKLYACRAKKKVQQLGDHPNIFEVRYNGGH 61
EDG+ W KYG K + YPRSYYRC K C KK+V++ + P Y G H
Sbjct: 607 EDGYRWRKYGQKAVKNSPYPRSYYRCTTQK---CTVKKRVERSYEDPTTVITTYEGQH 771
>TC84716 homologue to GP|17064160|gb|AAL35287.1 WRKY transcription factor 29
{Arabidopsis thaliana}, partial (21%)
Length = 524
Score = 54.3 bits (129), Expect = 1e-08
Identities = 26/57 (45%), Positives = 33/57 (57%)
Frame = +2
Query: 5 DGFTWTKYGMKEIPGFIYPRSYYRCNHVKLYACRAKKKVQQLGDHPNIFEVRYNGGH 61
D + W KYG K I G YPRSYYRC+ K C AKK+V++ P ++ V Y H
Sbjct: 356 DAWAWRKYGQKPIKGSPYPRSYYRCSSSK--GCLAKKQVEKNHLDPKVYLVTYTAEH 520
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.322 0.139 0.429
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 4,504,004
Number of Sequences: 36976
Number of extensions: 64128
Number of successful extensions: 312
Number of sequences better than 10.0: 87
Number of HSP's better than 10.0 without gapping: 303
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 306
length of query: 126
length of database: 9,014,727
effective HSP length: 85
effective length of query: 41
effective length of database: 5,871,767
effective search space: 240742447
effective search space used: 240742447
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 52 (24.6 bits)
Medicago: description of AC144725.4