Medicago
BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= AC144724.5 - phase: 0 /pseudo
         (534 letters)

Database: MTGI 
           36,976 sequences; 27,044,181 total letters

Searching..................................................done


                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

BF649369                                                               55  7e-08
TC78966 weakly similar to GP|16649051|gb|AAL24377.1 Unknown prot...    30  1.8
BQ144022 homologue to GP|22831315|dbj hypothetical protein~predi...    30  2.3
TC82447 similar to GP|14589311|emb|CAC43238. calcium binding pro...    29  5.2
TC91527 weakly similar to PIR|T45786|T45786 receptor-protein kin...    28  8.8

>BF649369 
          Length = 631

 Score = 55.1 bits (131), Expect = 7e-08
 Identities = 39/96 (40%), Positives = 54/96 (55%)
 Frame = +1

Query: 7   WKVQPYTGLIS*WKQRTICPGKSSRKG*LRAMEGGDWKILSKNWQH*NRVEMLKNLWRPL 66
           WKV    G   *WKQRTI PG++ R+ *       DW+I SKN+QH  ++E LK+L +  
Sbjct: 244 WKVLRSIGSTY*WKQRTIYPGRN*RRR*SLVTTVEDWRIHSKNYQHFVKLEALKSLSKLS 423

Query: 67  NFCPRR*DVFLKTST*GTL*VD*SHRFVGGFVL*IL 102
           +   RR + + +++   T *VD*       FV *IL
Sbjct: 424 SCYRRRLEGYRRSNIWVTS*VD*KLTSEDEFVR*IL 531


>TC78966 weakly similar to GP|16649051|gb|AAL24377.1 Unknown protein
           {Arabidopsis thaliana}, partial (33%)
          Length = 1022

 Score = 30.4 bits (67), Expect = 1.8
 Identities = 20/46 (43%), Positives = 25/46 (53%), Gaps = 4/46 (8%)
 Frame = +1

Query: 393 CNSCMKEKW-*RCWGKVASRRNTV--ISTHFWGTNK-VGWEWIGGG 434
           CN C KE++ * C   V + R     IS +    NK VGW W+GGG
Sbjct: 496 CNVCDKEEYD*CCCNCVKATRECT*NISINEKAYNKEVGWSWLGGG 633


>BQ144022 homologue to GP|22831315|dbj hypothetical protein~predicted by
           GlimmerM etc. {Oryza sativa (japonica cultivar-group)},
           partial (16%)
          Length = 1286

 Score = 30.0 bits (66), Expect = 2.3
 Identities = 20/60 (33%), Positives = 27/60 (44%)
 Frame = -3

Query: 368 VWTWC*ACRG*IPWVRY*WIGRLSLCNSCMKEKW*RCWGKVASRRNTVISTHFWGTNKVG 427
           VW  C  C   +  VR  W+G L LC    +EK+   W K    +  V+S   WG   +G
Sbjct: 180 VWVGCPRCFL-LRAVRLSWVGLLVLCFLFSEEKFCGVWPK----KRWVVSVWGWGAGALG 16


>TC82447 similar to GP|14589311|emb|CAC43238. calcium binding protein
           {Sesbania rostrata}, partial (79%)
          Length = 660

 Score = 28.9 bits (63), Expect = 5.2
 Identities = 10/23 (43%), Positives = 15/23 (64%)
 Frame = -3

Query: 399 EKW*RCWGKVASRRNTVISTHFW 421
           E+W*RCW +V    N  +S H++
Sbjct: 166 EEW*RCWSRVGFNWNGFVSCHWF 98


>TC91527 weakly similar to PIR|T45786|T45786 receptor-protein kinase-like
           protein - Arabidopsis thaliana, partial (17%)
          Length = 1205

 Score = 28.1 bits (61), Expect = 8.8
 Identities = 13/45 (28%), Positives = 30/45 (65%), Gaps = 1/45 (2%)
 Frame = +2

Query: 177 LYVITSFYWKKKERTRQ-DLRSGKGSATSTTTRWRKEGPRAYASS 220
           L ++  F +++K +T + + ++ K S +STT++W   GP ++A++
Sbjct: 629 LSIVVFFLYRRKRQTEEKNSKTTKDSKSSTTSKW---GPLSFATT 754


  Database: MTGI
    Posted date:  Oct 22, 2004  3:39 PM
  Number of letters in database: 27,044,181
  Number of sequences in database:  36,976
  
Lambda     K      H
   0.357    0.157    0.630 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 19,995,007
Number of Sequences: 36976
Number of extensions: 306510
Number of successful extensions: 3139
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 1650
Number of HSP's successfully gapped in prelim test: 106
Number of HSP's that attempted gapping in prelim test: 1447
Number of HSP's gapped (non-prelim): 1837
length of query: 534
length of database: 9,014,727
effective HSP length: 101
effective length of query: 433
effective length of database: 5,280,151
effective search space: 2286305383
effective search space used: 2286305383
frameshift window, decay const: 50,  0.1
T: 13
A: 40
X1: 14 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 37 (21.7 bits)
S2: 61 (28.1 bits)


Medicago: description of AC144724.5