BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC144644.6 + phase: 0
(235 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC81167 similar to SP|Q9SFC6|GDIR_ARATH Rho GDP-dissociation inh... 263 4e-71
TC79004 similar to GP|7228160|emb|CAB77025.1 putative Rho GDP di... 194 2e-50
TC93892 similar to SP|Q9SFC6|GDIR_ARATH Rho GDP-dissociation inh... 115 1e-26
AL385054 weakly similar to GP|14278159|pdb Chain A Crystal Stru... 87 4e-18
BG450538 similar to GP|7228160|emb| putative Rho GDP dissociatio... 70 7e-13
TC79702 MtN14 33 0.12
TC80288 weakly similar to PIR|T06029|T06029 hypothetical protein... 32 0.16
TC82370 similar to GP|15028377|gb|AAK76665.1 putative heat shock... 31 0.47
AW690594 similar to GP|23498163|emb hypothetical protein {Plasmo... 30 1.0
TC78700 similar to GP|21554835|gb|AAM63703.1 unknown {Arabidopsi... 29 1.8
TC79394 weakly similar to PIR|T49019|T49019 probable RNA binding... 29 1.8
TC77441 similar to GP|21592359|gb|AAM64310.1 unknown {Arabidopsi... 29 1.8
BG587062 similar to GP|19881001|gb chemiosmotic efflux system B ... 28 2.3
TC90913 similar to GP|19347877|gb|AAL85996.1 unknown protein {Ar... 28 3.0
TC77337 weakly similar to GP|4019275|gb|AAC95573.1| orf 48 {Atel... 28 4.0
TC91087 similar to SP|P18709|VTA2_XENLA Vitellogenin A2 precurso... 28 4.0
TC85840 similar to SP|P35016|ENPL_CATRO Endoplasmin homolog prec... 28 4.0
AA660309 similar to PIR|H84545|H84 probable ubiquitin-conjugatin... 28 4.0
TC79532 weakly similar to GP|8164030|gb|AAF73960.1| SGS3 {Arabid... 28 4.0
BG455051 similar to GP|9294321|dbj DEAD-box ATP-dependent RNA he... 27 5.2
>TC81167 similar to SP|Q9SFC6|GDIR_ARATH Rho GDP-dissociation inhibitor 1
(Rho GDI-1). [Mouse-ear cress] {Arabidopsis thaliana},
partial (77%)
Length = 1343
Score = 263 bits (672), Expect = 4e-71
Identities = 120/196 (61%), Positives = 160/196 (81%)
Frame = +3
Query: 36 YDEAEEIVPDEDEEEEPKLELDLGPQFSLKEQLEKDKDDESLRKWKEQLLGNVDVSAVGE 95
Y + E+E+++ +++LGPQ +LKEQLEKDKDDESLR+WKEQLLG+VD++AVGE
Sbjct: 375 YMSESSVAATEEEDDDEDRKIELGPQCTLKEQLEKDKDDESLRRWKEQLLGSVDINAVGE 554
Query: 96 KIEPEVKIVSLTIICQGRPDLILPIPFTADSKKSMFTLKEGSQYRLKFSFTVSNNIVSGL 155
+EPEVKI+SL I RPD++LPIP + K FTLKEGS+YRL F+F V++NIVSGL
Sbjct: 555 TLEPEVKILSLAIKAADRPDIVLPIPEDGNPKGLWFTLKEGSKYRLMFTFQVNHNIVSGL 734
Query: 156 RYTNVVWKTGVRVENTKKMLGTYSPQQEPYTYELEEEITPSGLFARGTYSARTKFVDDDR 215
+YTN VWKTG++V+++K+M+GT+SPQ E YT+E+ EE TPSG+FARG YSAR+KFVDDD
Sbjct: 735 KYTNTVWKTGIKVDSSKEMIGTFSPQTETYTHEMPEETTPSGMFARGAYSARSKFVDDDN 914
Query: 216 KCYLDASYRFEIQKNW 231
KCYL+ +Y F+I+K+W
Sbjct: 915 KCYLEINYTFDIRKDW 962
>TC79004 similar to GP|7228160|emb|CAB77025.1 putative Rho GDP dissociation
inhibitor {Nicotiana tabacum}, partial (76%)
Length = 1015
Score = 194 bits (494), Expect = 2e-50
Identities = 93/193 (48%), Positives = 144/193 (74%), Gaps = 1/193 (0%)
Frame = +1
Query: 43 VPDEDEEEEPKLELDLGPQFSLKEQLEKDKDDESLRKWKEQLLGNVDVSAVGEKIEPEVK 102
V EDEE+E ++ + P SLKEQ+EKDK+DESLR+WKE+LLG+++ S + +++EPEVK
Sbjct: 208 VASEDEEDEGQVFVP-SPLLSLKEQIEKDKEDESLRRWKEKLLGSLE-SDLDDQLEPEVK 381
Query: 103 IVSLTIICQGRPDLILPIPFTA-DSKKSMFTLKEGSQYRLKFSFTVSNNIVSGLRYTNVV 161
S+ I+ + +++ P+P + + +FTL+EGS+Y+LK F+V +N+VSGL Y+N V
Sbjct: 382 FHSIGILSEDFGEIVTPLPVEEHQNSRMLFTLREGSRYQLKLQFSVMHNLVSGLTYSNTV 561
Query: 162 WKTGVRVENTKKMLGTYSPQQEPYTYELEEEITPSGLFARGTYSARTKFVDDDRKCYLDA 221
WK G++V+ +K MLGT++PQ+ PY Y L+E+ TP+G ARG YSA+ KF DDD++C+++
Sbjct: 562 WKGGLQVDQSKGMLGTFAPQKGPYVYALKEDTTPAGALARGVYSAKLKFEDDDKRCHMEL 741
Query: 222 SYRFEIQKNWPTP 234
Y FEI+K+ +P
Sbjct: 742 KYLFEIKKSG*SP 780
>TC93892 similar to SP|Q9SFC6|GDIR_ARATH Rho GDP-dissociation inhibitor 1
(Rho GDI-1). [Mouse-ear cress] {Arabidopsis thaliana},
partial (28%)
Length = 441
Score = 115 bits (288), Expect = 1e-26
Identities = 50/69 (72%), Positives = 63/69 (90%)
Frame = +2
Query: 163 KTGVRVENTKKMLGTYSPQQEPYTYELEEEITPSGLFARGTYSARTKFVDDDRKCYLDAS 222
KTG++V++TK+M+GT+SPQ EPYT+E+ EE TPSGLFARGTYSARTKFVDDD+K YLD S
Sbjct: 2 KTGIKVDSTKEMIGTFSPQAEPYTHEMPEETTPSGLFARGTYSARTKFVDDDKKSYLDIS 181
Query: 223 YRFEIQKNW 231
Y F+I+K+W
Sbjct: 182 YTFDIRKDW 208
>AL385054 weakly similar to GP|14278159|pdb Chain A Crystal Structure Of
Truncated Human Rhogdi Quadruple Mutant, partial (45%)
Length = 337
Score = 87.4 bits (215), Expect = 4e-18
Identities = 37/81 (45%), Positives = 58/81 (70%)
Frame = +3
Query: 151 IVSGLRYTNVVWKTGVRVENTKKMLGTYSPQQEPYTYELEEEITPSGLFARGTYSARTKF 210
+VSGL+Y VV + G+RV+ T++M+G+Y P EPY + E PSG+ ARG Y A++KF
Sbjct: 3 VVSGLKYLQVVKRKGIRVDKTEEMIGSYGPNIEPYEKKFMVEEAPSGMLARGHYDAKSKF 182
Query: 211 VDDDRKCYLDASYRFEIQKNW 231
+DDD +++ S+ F+I+K+W
Sbjct: 183 IDDDNVTHIEWSWSFDIKKDW 245
>BG450538 similar to GP|7228160|emb| putative Rho GDP dissociation inhibitor
{Nicotiana tabacum}, partial (32%)
Length = 599
Score = 70.1 bits (170), Expect = 7e-13
Identities = 31/74 (41%), Positives = 53/74 (70%), Gaps = 1/74 (1%)
Frame = +2
Query: 95 EKIEPEVKIVSLTIICQGRPDLILPIPFTA-DSKKSMFTLKEGSQYRLKFSFTVSNNIVS 153
+++EPEVK S+ I+ + +++ P+P + + +FTL+EGS+Y+LK F+V +N+VS
Sbjct: 35 DQLEPEVKFHSIGILSEDFGEIVTPLPVEEHQNSRMLFTLREGSRYQLKLQFSVMHNLVS 214
Query: 154 GLRYTNVVWKTGVR 167
GL Y+N VWK G++
Sbjct: 215 GLTYSNTVWKGGLQ 256
>TC79702 MtN14
Length = 973
Score = 32.7 bits (73), Expect = 0.12
Identities = 30/104 (28%), Positives = 54/104 (51%), Gaps = 1/104 (0%)
Frame = +2
Query: 40 EEIVPDEDEEEEPKLELDLGPQFSLKEQLEKDKDD-ESLRKWKEQLLGNVDVSAVGEKIE 98
E +VP +D EEE K E Q KE +E+ K++ SL + + G+ V AV EK
Sbjct: 254 EVVVPVKDTEEETKKE----EQTETKEPVEETKENGNSLNVEETKENGDSVVEAVQEKPA 421
Query: 99 PEVKIVSLTIICQGRPDLILPIPFTADSKKSMFTLKEGSQYRLK 142
E + V++ + + ++ P T D+ K+ T +E ++ +++
Sbjct: 422 EESETVNVV-----KDENVVAEPETKDNVKTEETSEEKNEEKVE 538
>TC80288 weakly similar to PIR|T06029|T06029 hypothetical protein T28I19.100
- Arabidopsis thaliana, partial (14%)
Length = 1460
Score = 32.3 bits (72), Expect = 0.16
Identities = 19/74 (25%), Positives = 36/74 (47%)
Frame = +3
Query: 10 TTKDVSFNAHLEEEHKNKGTNKVGVNYDEAEEIVPDEDEEEEPKLELDLGPQFSLKEQLE 69
T ++ N H E +++ NK G E E +E E+E +E+D K + +
Sbjct: 501 TEEESEDNVHERREEQDEEENKHGAEVQEENESKSEEVEDEGGDVEIDENDH--EKSEAD 674
Query: 70 KDKDDESLRKWKEQ 83
D++DE + + K++
Sbjct: 675 NDREDEVVDEEKDK 716
>TC82370 similar to GP|15028377|gb|AAK76665.1 putative heat shock factor
protein hsf8 {Arabidopsis thaliana}, partial (7%)
Length = 645
Score = 30.8 bits (68), Expect = 0.47
Identities = 24/71 (33%), Positives = 34/71 (47%), Gaps = 4/71 (5%)
Frame = +2
Query: 45 DEDEEEEPKLELDLGPQFSLKEQ----LEKDKDDESLRKWKEQLLGNVDVSAVGEKIEPE 100
D DE+ + L + P FSLK Q EK D LRK + NV+ K EP
Sbjct: 404 DGDEDVDMSSRLRMKPNFSLKMQSGQVKEKFSDMTLLRKPGSLIGKNVESE*KTRKEEPS 583
Query: 101 VKIVSLTIICQ 111
++ +LT++ Q
Sbjct: 584 YEVSNLTLLEQ 616
>AW690594 similar to GP|23498163|emb hypothetical protein {Plasmodium
falciparum 3D7}, partial (10%)
Length = 633
Score = 29.6 bits (65), Expect = 1.0
Identities = 19/69 (27%), Positives = 36/69 (51%)
Frame = +3
Query: 7 AVSTTKDVSFNAHLEEEHKNKGTNKVGVNYDEAEEIVPDEDEEEEPKLELDLGPQFSLKE 66
A S+ K H EEE + + + + +E E+ +E+EEEE + E D +E
Sbjct: 15 AESSWKRRKVEEHNEEEDEGEVEEEEDEHDEEEEDEHDEEEEEEEEEEEEDEHDDEEEEE 194
Query: 67 QLEKDKDDE 75
+ E+++++E
Sbjct: 195EEEEEEEEE 221
Score = 26.6 bits (57), Expect = 8.9
Identities = 17/54 (31%), Positives = 29/54 (53%)
Frame = +3
Query: 22 EEHKNKGTNKVGVNYDEAEEIVPDEDEEEEPKLELDLGPQFSLKEQLEKDKDDE 75
EE +++ + +DE EE +E+EEEE D + +E+ E++ DDE
Sbjct: 81 EEEEDEHDEEEEDEHDEEEE---EEEEEEEEDEHDDEEEEEEEEEEEEEEDDDE 233
>TC78700 similar to GP|21554835|gb|AAM63703.1 unknown {Arabidopsis
thaliana}, partial (5%)
Length = 687
Score = 28.9 bits (63), Expect = 1.8
Identities = 16/51 (31%), Positives = 29/51 (56%)
Frame = +2
Query: 48 EEEEPKLELDLGPQFSLKEQLEKDKDDESLRKWKEQLLGNVDVSAVGEKIE 98
EEEE + E++L + ++ +K K DE ++W+E+ G V+ E +E
Sbjct: 443 EEEEKEKEVNLDGEGVGDKKKKKRKRDEVEKEWEEKKYGIVEGGDKDESVE 595
>TC79394 weakly similar to PIR|T49019|T49019 probable RNA binding protein -
Arabidopsis thaliana, partial (74%)
Length = 1909
Score = 28.9 bits (63), Expect = 1.8
Identities = 19/69 (27%), Positives = 38/69 (54%), Gaps = 3/69 (4%)
Frame = +2
Query: 20 LEEEHKNKGTNKVGVNYDEAE---EIVPDEDEEEEPKLELDLGPQFSLKEQLEKDKDDES 76
L+ ++ + +++ V Y+E E E+ +E+EEEE ++E +E D++DE+
Sbjct: 242 LDGDNDQEESSEEEVEYEEVEVEEEVEEEEEEEEEEEVE---------EESKPLDEEDEA 394
Query: 77 LRKWKEQLL 85
+K +LL
Sbjct: 395 DKKKHAELL 421
>TC77441 similar to GP|21592359|gb|AAM64310.1 unknown {Arabidopsis
thaliana}, partial (98%)
Length = 1046
Score = 28.9 bits (63), Expect = 1.8
Identities = 15/50 (30%), Positives = 29/50 (58%)
Frame = +3
Query: 34 VNYDEAEEIVPDEDEEEEPKLELDLGPQFSLKEQLEKDKDDESLRKWKEQ 83
V+ D+++ V +DE ++ E D + EQ++K++ E LRK ++Q
Sbjct: 411 VDADDSDVEVNSDDESDDDDDEDDTEALLAELEQIKKERAQEKLRKEQQQ 560
>BG587062 similar to GP|19881001|gb chemiosmotic efflux system B protein A
{Legionella pneumophila}, partial (1%)
Length = 780
Score = 28.5 bits (62), Expect = 2.3
Identities = 21/70 (30%), Positives = 38/70 (54%), Gaps = 14/70 (20%)
Frame = +3
Query: 22 EEHKNKGTNKVGVNYDEAEEIVPDEDEEEEPKLELDLG------PQFSLKE--------Q 67
++++NK NK +N +E + + + + EEPK L+LG P L++ +
Sbjct: 294 KKNRNKNKNKSMINAEEPKPAL-ELGKNEEPKPALELGKNEEPKPVLELEKNEEPKPALE 470
Query: 68 LEKDKDDESL 77
LEK+K+ ES+
Sbjct: 471 LEKNKEVESI 500
>TC90913 similar to GP|19347877|gb|AAL85996.1 unknown protein {Arabidopsis
thaliana}, partial (32%)
Length = 1098
Score = 28.1 bits (61), Expect = 3.0
Identities = 23/74 (31%), Positives = 38/74 (51%), Gaps = 11/74 (14%)
Frame = +3
Query: 30 NKVGVNYDE-AEEIVPDEDEEEEPKLELDLGPQFSLKEQ----------LEKDKDDESLR 78
N +G+ D+ EE V E EEE+ EL +F +Q ++D+ ++ R
Sbjct: 366 NWLGLREDDMVEEDVEFEVEEEQ---ELSENEKFEAWKQRAEAIIDIREAQEDRKNQDHR 536
Query: 79 KWKEQLLGNVDVSA 92
KW++ LL + DVS+
Sbjct: 537 KWEDWLLEDEDVSS 578
>TC77337 weakly similar to GP|4019275|gb|AAC95573.1| orf 48 {Ateline
herpesvirus 3}, partial (8%)
Length = 986
Score = 27.7 bits (60), Expect = 4.0
Identities = 18/63 (28%), Positives = 37/63 (58%), Gaps = 1/63 (1%)
Frame = +3
Query: 22 EEHKNKGTNKVGVNYDEAEEIVPDEDEEEEPKLE-LDLGPQFSLKEQLEKDKDDESLRKW 80
+E+ + ++ G + DE ++ D+D+EEE E + G E+ E+D+D+E+L+
Sbjct: 582 DENGEEEDDEDGDDQDEDDDEDEDDDDEEEGGEEDEEEGVDEEDNEEEEEDEDEEALQPP 761
Query: 81 KEQ 83
K++
Sbjct: 762 KKR 770
>TC91087 similar to SP|P18709|VTA2_XENLA Vitellogenin A2 precursor (VTG A2),
partial (0%)
Length = 652
Score = 27.7 bits (60), Expect = 4.0
Identities = 26/110 (23%), Positives = 45/110 (40%), Gaps = 17/110 (15%)
Frame = -3
Query: 15 SFNAHLEEEHKNKGTNKVGVNYDEAEEIVPDEDEEE-----------EPKLELDLGPQFS 63
SF E+E G N+DE E+V D D+E+ +L G QF
Sbjct: 371 SFQKLPEDEEVEMPDFNEGDNHDELGEVVADSDDEDTVSEDEGSTILSTRLLQRYGSQFL 192
Query: 64 LKEQLEKDK-DDESLRKWKEQ-----LLGNVDVSAVGEKIEPEVKIVSLT 107
++++ K E+L ++E + S + K +P + + S+T
Sbjct: 191 RNDEVKDAKRKSEALLCFQESASSHATFSKANSSRIWNKAKPRISLSSVT 42
>TC85840 similar to SP|P35016|ENPL_CATRO Endoplasmin homolog precursor
(GRP94 homolog). [Rosy periwinkle Madagascar
periwinkle], partial (92%)
Length = 2791
Score = 27.7 bits (60), Expect = 4.0
Identities = 19/50 (38%), Positives = 24/50 (48%)
Frame = +1
Query: 32 VGVNYDEAEEIVPDEDEEEEPKLELDLGPQFSLKEQLEKDKDDESLRKWK 81
V V DE +E +ED E PK E S E +KD+D+E K K
Sbjct: 916 VEVPADEDDESSEEEDTTESPKEE-------SEDEDADKDEDEEKKPKTK 1044
>AA660309 similar to PIR|H84545|H84 probable ubiquitin-conjugating enzyme
[imported] - Arabidopsis thaliana, partial (4%)
Length = 600
Score = 27.7 bits (60), Expect = 4.0
Identities = 19/75 (25%), Positives = 33/75 (43%)
Frame = +1
Query: 2 SAAVGAVSTTKDVSFNAHLEEEHKNKGTNKVGVNYDEAEEIVPDEDEEEEPKLELDLGPQ 61
+A VS K + + ++ KNK T VG+ E+ D D + + + D G
Sbjct: 166 NAEPDGVSCKKSNTMQIYRQDVVKNKNTGMVGI----VTEVNGDSDSDSDSDSDTDSGVT 333
Query: 62 FSLKEQLEKDKDDES 76
E+ + ++ DES
Sbjct: 334 DDEGEEGDVEEGDES 378
>TC79532 weakly similar to GP|8164030|gb|AAF73960.1| SGS3 {Arabidopsis
thaliana}, partial (47%)
Length = 1558
Score = 27.7 bits (60), Expect = 4.0
Identities = 22/86 (25%), Positives = 39/86 (44%), Gaps = 5/86 (5%)
Frame = +1
Query: 37 DEAEEIVPDEDEEEEPKLELDLGPQFSLKEQLEKDKDDESLR-----KWKEQLLGNVDVS 91
++ EE DE+EEE+ +L+ + ++ + D +S KW ++ N+D
Sbjct: 649 NDGEEDDDDEEEEEDDCDDLEDTDDDLMSDEYDSDASQKSHETRKKSKWFKKFFQNLDNL 828
Query: 92 AVGEKIEPEVKIVSLTIICQGRPDLI 117
+ + EPE + CQG P I
Sbjct: 829 TIDQINEPERQWHCPA--CQGGPGAI 900
>BG455051 similar to GP|9294321|dbj DEAD-box ATP-dependent RNA helicase
{Arabidopsis thaliana}, partial (30%)
Length = 672
Score = 27.3 bits (59), Expect = 5.2
Identities = 16/60 (26%), Positives = 28/60 (46%)
Frame = +2
Query: 20 LEEEHKNKGTNKVGVNYDEAEEIVPDEDEEEEPKLELDLGPQFSLKEQLEKDKDDESLRK 79
+ + H N+ ++K E E++V EDE EE K++ + G E + E+ K
Sbjct: 20 VNDTHSNEESSKKKKKNKEEEDVVEVEDELEEKKVKNNKGSGIMSSESFSSLELSENTSK 199
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.311 0.132 0.374
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 6,375,112
Number of Sequences: 36976
Number of extensions: 73376
Number of successful extensions: 377
Number of sequences better than 10.0: 48
Number of HSP's better than 10.0 without gapping: 351
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 363
length of query: 235
length of database: 9,014,727
effective HSP length: 93
effective length of query: 142
effective length of database: 5,575,959
effective search space: 791786178
effective search space used: 791786178
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.8 bits)
S2: 57 (26.6 bits)
Medicago: description of AC144644.6