BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC144618.9 + phase: 0
(313 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC77296 similar to GP|16323145|gb|AAL15307.1 AT5g11000/T30N20_27... 143 1e-34
CB892694 similar to GP|20804936|dbj hypothetical protein~similar... 137 4e-33
BG448659 weakly similar to GP|8777417|dbj| gene_id:MIF21.16~pir|... 90 1e-18
CB891510 similar to GP|19699077|gb AT5g28150/T24G3_80 {Arabidops... 81 5e-16
BQ147264 similar to GP|21805715|gb| hypothetical protein {Arabid... 62 3e-10
AW256652 53 2e-07
BF519170 similar to GP|21805715|gb| hypothetical protein {Arabid... 51 5e-07
BQ148897 similar to GP|10177387|dbj gene_id:MNL12.7~unknown prot... 31 0.54
TC79429 similar to PIR|T03803|T03803 tumor-related protein clon... 30 0.92
TC85937 similar to SP|P52711|CP23_HORVU Serine carboxypeptidase ... 29 2.7
BG457238 weakly similar to GP|19699077|gb| AT5g28150/T24G3_80 {A... 28 3.5
TC85916 similar to GP|9758968|dbj|BAB09411.1 26S proteasome p55 ... 28 4.6
AW683905 weakly similar to GP|9757854|dbj| contains similarity t... 28 6.0
AL367045 27 7.8
>TC77296 similar to GP|16323145|gb|AAL15307.1 AT5g11000/T30N20_270
{Arabidopsis thaliana}, partial (31%)
Length = 1293
Score = 143 bits (360), Expect = 1e-34
Identities = 85/263 (32%), Positives = 144/263 (54%), Gaps = 13/263 (4%)
Frame = +2
Query: 31 PTKTAQSTVTCLYQTSIGGQWRNISVLWCKNLINHTLNLKVD-----------SARGEQF 79
P T + T LYQ+++G +S+ W ++++ +L++++ S+ F
Sbjct: 218 PHSTNPNLTTYLYQSNVG----LVSLTWSRSILGRSLHVQLHHHPFDSPPTNPSSSSSSF 385
Query: 80 HQNCKIEVKPWCFWNRKGYRSFDVDGNQIDVYWDLRSAKFAGNCPEPCSDYYVMLVSDEE 139
H + +KP+ FW + G + + ++W+L AKF G+ PEP S +Y +V D E
Sbjct: 386 H----LHIKPFIFWKKHGTKKLS---SNTHLFWNLSKAKF-GSGPEPDSGFYFAVVVDNE 541
Query: 140 VVLLLGDYKKKAYKRMKMRQALVEATLLV-KRENVFAKKSFSTKAKFDEKRKESDIVVES 198
+ LL+GD K AY + K ++ LV KRE+V+A K ++T+A+F K +E ++
Sbjct: 542 MTLLVGDSNKDAYAKSKSKEHKNNFQYLVMKREHVYANKIYNTRARFGGKMRE----IQI 709
Query: 199 STAGNKDPEMWISIDGIVLIHIKNLQWKFRGNQTVMVNKQPVHVFWDVYDWLFSVSGS-G 257
G + S+DG ++ IK L+WKFRGN+ V ++ PV + WDVY+WLF S G
Sbjct: 710 DCGGRDHSRLCFSVDGQKVLQIKRLKWKFRGNERVEIDGVPVQISWDVYNWLFVKDNSDG 889
Query: 258 PGLFIFKPGPTEVESDKEEKGLE 280
+F+FK E + D++++G E
Sbjct: 890 HAIFMFK--FEEEDEDEQQRGKE 952
>CB892694 similar to GP|20804936|dbj hypothetical protein~similar to
Arabidopsis thaliana chromosome 4 At3g43920At4g12690,
partial (20%)
Length = 843
Score = 137 bits (346), Expect = 4e-33
Identities = 85/224 (37%), Positives = 120/224 (52%), Gaps = 40/224 (17%)
Frame = +1
Query: 130 YYVMLVSDEEVVLLLGDYKKKAYKRMKMRQALVEATLLVKRENVFAKKSFSTKAKFDEKR 189
YYV +V D+EVV LLGD KK+AY+R R +L++ L+ K+E++F K+ FSTKAKF EK
Sbjct: 4 YYVAVVCDQEVVSLLGDLKKEAYRRTGCRPSLIDPILVSKKEHIFGKRKFSTKAKFHEKG 183
Query: 190 KESDIVV----ESSTAGNK----------------DPEMWISIDGIVLIHIKNLQWKFRG 229
+ +I + + S +GN PEM I DG +++H+K+LQWKFRG
Sbjct: 184 RCHEISIKCKNKGSNSGNNVVGDGDSVMIGGGVQVQPEMEIRFDGHLVVHVKHLQWKFRG 363
Query: 230 NQTVMVNKQPVHVFWDVYDWLFSVSGSGPGLFIFKP--------------------GPTE 269
N+ + +NK V V+WDV+DWLFS G LFIFKP +
Sbjct: 364 NELIHLNKMRVEVYWDVHDWLFS-PGLKHALFIFKPVFTCHSMSPFSLFSPSLSCSSSST 540
Query: 270 VESDKEEKGLEGESDDGSATSGYYSTKSYTPFESCLVLCAYKLE 313
E + +S +G + S S+ S E CL L A+K+E
Sbjct: 541 TPLSNETRSSTCDSVEGRSVSDSLSSSS----EYCLFLYAWKVE 660
>BG448659 weakly similar to GP|8777417|dbj|
gene_id:MIF21.16~pir||T06627~similar to unknown protein
{Arabidopsis thaliana}, partial (9%)
Length = 348
Score = 90.1 bits (222), Expect = 1e-18
Identities = 37/89 (41%), Positives = 61/89 (67%)
Frame = +1
Query: 33 KTAQSTVTCLYQTSIGGQWRNISVLWCKNLINHTLNLKVDSARGEQFHQNCKIEVKPWCF 92
++ ++ +Y+T + G R I++ WCKNL+ H L++ V++ E + +CK+E+KPW F
Sbjct: 73 RSGKTVYMSVYRTKLAGHCRLITITWCKNLMLHGLSVTVENPEKETQY-SCKVELKPWYF 249
Query: 93 WNRKGYRSFDVDGNQIDVYWDLRSAKFAG 121
W ++G + F VDG +DV+WDL+SAKF G
Sbjct: 250 WRKQGSKRFTVDGKAVDVFWDLKSAKFNG 336
>CB891510 similar to GP|19699077|gb AT5g28150/T24G3_80 {Arabidopsis
thaliana}, partial (40%)
Length = 462
Score = 81.3 bits (199), Expect = 5e-16
Identities = 41/95 (43%), Positives = 60/95 (63%)
Frame = +2
Query: 32 TKTAQSTVTCLYQTSIGGQWRNISVLWCKNLINHTLNLKVDSARGEQFHQNCKIEVKPWC 91
+KTAQ+ V C+YQ SI G+ I++ W K+L+ ++ +D A + CK+++KPW
Sbjct: 182 SKTAQNLVICVYQCSIRGRSCLITITWSKSLMGQGFSVGIDDASNQCL---CKVDIKPWV 352
Query: 92 FWNRKGYRSFDVDGNQIDVYWDLRSAKFAGNCPEP 126
F RKG +S + +IDVYWDL SA+F G PEP
Sbjct: 353 FSKRKGCKSLEAYSCKIDVYWDLSSARF-GVGPEP 454
>BQ147264 similar to GP|21805715|gb| hypothetical protein {Arabidopsis
thaliana}, partial (15%)
Length = 646
Score = 62.0 bits (149), Expect = 3e-10
Identities = 40/122 (32%), Positives = 62/122 (50%), Gaps = 3/122 (2%)
Frame = +1
Query: 54 ISVLWCKNLINHTLNLKV---DSARGEQFHQNCKIEVKPWCFWNRKGYRSFDVDGNQIDV 110
I+V WCKN N L++ D + F N F +KG + D + ++ V
Sbjct: 280 ITVTWCKNHSNQGLSISFGDEDPSTVPSFRLNTNSRF----FRKKKGSKMLDFEDFKVTV 447
Query: 111 YWDLRSAKFAGNCPEPCSDYYVMLVSDEEVVLLLGDYKKKAYKRMKMRQALVEATLLVKR 170
WDL +AK+ PEP +YV++V D E+ L+LGD + K+MK + + LL +R
Sbjct: 448 LWDLSNAKYETG-PEPVDAFYVVIVVDSEIGLILGD-SETVTKKMKDSVPMAKIXLLSRR 621
Query: 171 EN 172
E+
Sbjct: 622 EH 627
>AW256652
Length = 611
Score = 52.8 bits (125), Expect = 2e-07
Identities = 23/59 (38%), Positives = 35/59 (58%)
Frame = +1
Query: 32 TKTAQSTVTCLYQTSIGGQWRNISVLWCKNLINHTLNLKVDSARGEQFHQNCKIEVKPW 90
T++ QS +Y+T + R I++ WCKNL+ H L++ V+ GE CK E+KPW
Sbjct: 436 TRSGQSVYMSVYRTKVADHCRLITITWCKNLLLHGLSISVEGPEGEA-QYTCKGELKPW 609
>BF519170 similar to GP|21805715|gb| hypothetical protein {Arabidopsis
thaliana}, partial (14%)
Length = 560
Score = 51.2 bits (121), Expect = 5e-07
Identities = 22/64 (34%), Positives = 40/64 (62%), Gaps = 2/64 (3%)
Frame = -1
Query: 203 NKDPEMWISIDGIVLIHIKNLQWKFRGNQTVMVNKQPVHVFWDVYDWLFSVSG--SGPGL 260
+K + + +D ++ +K L+W FRGNQT+ V+ V + WD++DW+F+ S S +
Sbjct: 551 SKSHMLCLFMDKKIVFKVKRLKWNFRGNQTIFVDGLVVDMMWDLHDWIFNPSNIDSVSAV 372
Query: 261 FIFK 264
F+F+
Sbjct: 371 FMFR 360
Score = 27.7 bits (60), Expect = 6.0
Identities = 10/22 (45%), Positives = 15/22 (67%)
Frame = -1
Query: 93 WNRKGYRSFDVDGNQIDVYWDL 114
WN +G ++ VDG +D+ WDL
Sbjct: 485 WNFRGNQTIFVDGLVVDMMWDL 420
>BQ148897 similar to GP|10177387|dbj gene_id:MNL12.7~unknown protein
{Arabidopsis thaliana}, partial (48%)
Length = 620
Score = 31.2 bits (69), Expect = 0.54
Identities = 11/19 (57%), Positives = 15/19 (78%)
Frame = +3
Query: 51 WRNISVLWCKNLINHTLNL 69
WRN ++L CK L+NH L+L
Sbjct: 528 WRNYAILICKQLVNHMLSL 584
>TC79429 similar to PIR|T03803|T03803 tumor-related protein clone NF34 -
common tobacco, partial (42%)
Length = 807
Score = 30.4 bits (67), Expect = 0.92
Identities = 14/34 (41%), Positives = 17/34 (49%)
Frame = -3
Query: 230 NQTVMVNKQPVHVFWDVYDWLFSVSGSGPGLFIF 263
N+T N +P V W +DW V S P LF F
Sbjct: 223 NKTTTNNTRPTTVTWGCWDWSDVVVYSSPNLFAF 122
>TC85937 similar to SP|P52711|CP23_HORVU Serine carboxypeptidase II-3
precursor (EC 3.4.16.6) (CP-MII.3). [Barley] {Hordeum
vulgare}, partial (44%)
Length = 1950
Score = 28.9 bits (63), Expect = 2.7
Identities = 32/116 (27%), Positives = 47/116 (39%), Gaps = 16/116 (13%)
Frame = +3
Query: 33 KTAQSTVTCLYQTSIGGQW----RNISVLWCKNLINHTLNLKVDSARGEQFHQNCKIEVK 88
K +T+ L SIG W N+ ++ NL H LN E++ K V
Sbjct: 753 KLYNNTIINLKGISIGNAWIDDATNLKGIY-DNLWTHALNSDQTHELIEKYCDFTKENVS 929
Query: 89 PWCFWNRKGYRSFDVDGNQIDVY------------WDLRSAKFAGNCPEPCSDYYV 132
C N ++F V+ +ID+Y + S + N +PCSDYYV
Sbjct: 930 AIC--NNATDKAF-VETGKIDIYNIHAPLCHDSSLKNGSSTGYVSNDFDPCSDYYV 1088
>BG457238 weakly similar to GP|19699077|gb| AT5g28150/T24G3_80 {Arabidopsis
thaliana}, partial (22%)
Length = 656
Score = 28.5 bits (62), Expect = 3.5
Identities = 12/40 (30%), Positives = 22/40 (55%)
Frame = +1
Query: 33 KTAQSTVTCLYQTSIGGQWRNISVLWCKNLINHTLNLKVD 72
+ Q+ TC+YQ + + I+ W KNL+ L++ +D
Sbjct: 532 RXPQNVGTCIYQXKLRXRSCLITXSWTKNLMGQGLSVGID 651
>TC85916 similar to GP|9758968|dbj|BAB09411.1 26S proteasome p55 protein-like
{Arabidopsis thaliana}, partial (95%)
Length = 1807
Score = 28.1 bits (61), Expect = 4.6
Identities = 14/50 (28%), Positives = 26/50 (52%)
Frame = +3
Query: 17 LASLMSQRITEEPLPTKTAQSTVTCLYQTSIGGQWRNISVLWCKNLINHT 66
L L QR+T++ + + Q + +Y T WR+ +LW + +I +T
Sbjct: 1194 LPRLWLQRLTDQWE*SVSKQQRIAMMYLTHGQQTWRSCLILWRRAVIKYT 1343
>AW683905 weakly similar to GP|9757854|dbj| contains similarity to apoptosis
antagonizing transcription factor~gene_id:MFB13.10,
partial (12%)
Length = 492
Score = 27.7 bits (60), Expect = 6.0
Identities = 13/26 (50%), Positives = 18/26 (69%), Gaps = 1/26 (3%)
Frame = +3
Query: 268 TEVESDKEEKGL-EGESDDGSATSGY 292
+E+E +KEE G EGE DDG +G+
Sbjct: 186 SELEEEKEEDGEDEGEEDDGQEENGW 263
>AL367045
Length = 427
Score = 27.3 bits (59), Expect = 7.8
Identities = 13/41 (31%), Positives = 20/41 (48%)
Frame = +1
Query: 223 LQWKFRGNQTVMVNKQPVHVFWDVYDWLFSVSGSGPGLFIF 263
+ W R + VM +K+P ++W + SGSG L F
Sbjct: 172 VDWYKRWSYIVMTSKEPTELWWTAVSLVGRQSGSGLYLISF 294
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.317 0.133 0.407
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 10,725,120
Number of Sequences: 36976
Number of extensions: 161326
Number of successful extensions: 838
Number of sequences better than 10.0: 28
Number of HSP's better than 10.0 without gapping: 825
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 829
length of query: 313
length of database: 9,014,727
effective HSP length: 96
effective length of query: 217
effective length of database: 5,465,031
effective search space: 1185911727
effective search space used: 1185911727
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 58 (26.9 bits)
Medicago: description of AC144618.9