BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC144618.2 + phase: 0
(106 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BF520646 similar to GP|21741986|emb OSJNBa0060P14.7 {Oryza sativ... 141 3e-35
AL389791 65 5e-12
TC77768 weakly similar to GP|22002137|gb|AAM88621.1 hypothetical... 45 3e-06
TC77767 weakly similar to GP|22002137|gb|AAM88621.1 hypothetical... 42 5e-05
AW126356 similar to GP|22758342|gb| hypothetical protein {Oryza ... 40 1e-04
TC81137 similar to GP|13543783|gb|AAH06040.1 Unknown (protein fo... 39 3e-04
TC76522 homologue to SP|O82709|CALX_PEA Calnexin homolog precurs... 32 0.038
TC91295 similar to PIR|T46192|T46192 acetylglutamate kinase-like... 28 0.55
BQ141029 similar to GP|8132441|gb| extensin {Pisum sativum}, par... 26 2.1
TC77769 similar to PIR|T06397|T06397 isoprenylated protein FP6 -... 26 2.7
CA921270 similar to GP|18481964|gb Putative receptor-like protei... 25 4.7
TC88328 similar to GP|3287270|emb|CAA70725.1 involved in starch ... 25 4.7
TC91688 25 4.7
TC87077 similar to PIR|A86407|A86407 unknown protein [imported] ... 25 6.1
BI311908 similar to GP|10177105|d gene_id:MXC20.14~pir||T13959~s... 25 6.1
TC77658 similar to PIR|T46895|T46895 acyl-CoA dehydrogenase (EC ... 24 8.0
TC90272 similar to PIR|T45686|T45686 receptor-like protein kinas... 24 8.0
>BF520646 similar to GP|21741986|emb OSJNBa0060P14.7 {Oryza sativa}, partial
(26%)
Length = 590
Score = 141 bits (356), Expect = 3e-35
Identities = 77/111 (69%), Positives = 88/111 (78%), Gaps = 5/111 (4%)
Frame = +3
Query: 1 MNNQKLRNKAMAIAVGVSGVEGTTIKGDNKDQIEVTGEEIDSVKLASLLRKKFGYADLVS 60
MNNQKLR+KAM IA GVSGVEGT I+G+NKDQIEVTGE+IDSV+L SLLRKKF +A+LVS
Sbjct: 72 MNNQKLRSKAMTIAAGVSGVEGTAIQGENKDQIEVTGEQIDSVRLTSLLRKKFCHAELVS 251
Query: 61 IEAVGKTEEKKDKEKAEAIVAWPYVYSSVPHYPVYEIKN-----EPSCSIM 106
+ VGKTEEK K EAIVAW Y P+YPVYEI+N +PSCSIM
Sbjct: 252 VGPVGKTEEK----KVEAIVAWNY---GAPNYPVYEIRNSYQYEDPSCSIM 383
>AL389791
Length = 276
Score = 64.7 bits (156), Expect = 5e-12
Identities = 30/66 (45%), Positives = 47/66 (70%)
Frame = +3
Query: 1 MNNQKLRNKAMAIAVGVSGVEGTTIKGDNKDQIEVTGEEIDSVKLASLLRKKFGYADLVS 60
M+ +K RNKA+ A V GV +++GD+KD+I VTG+ +D+V LA++L+KKF ++S
Sbjct: 78 MDCEKCRNKALKTAAEVKGVTSVSLEGDDKDKISVTGDNVDTVCLANMLKKKFNCVTILS 257
Query: 61 IEAVGK 66
+E V K
Sbjct: 258 VEEVKK 275
>TC77768 weakly similar to GP|22002137|gb|AAM88621.1 hypothetical protein
{Oryza sativa (japonica cultivar-group)}, partial (36%)
Length = 757
Score = 45.4 bits (106), Expect = 3e-06
Identities = 33/110 (30%), Positives = 57/110 (51%), Gaps = 12/110 (10%)
Frame = +3
Query: 1 MNNQKLRNKAMAIAVGVSGVEGTTIKGDNKDQIEVTGEEIDSVKLASLLRKKFGYADLVS 60
+N +++ KAM G+SGVE +I D KD+ +ID V++ + LR K YA+++S
Sbjct: 180 INEDRIKQKAMKAVSGLSGVESVSI--DMKDKKMTLIGDIDPVRVVAKLR-KICYAEILS 350
Query: 61 I---EAVGKTEEKKDKE---------KAEAIVAWPYVYSSVPHYPVYEIK 98
+ + K E KKD + K E ++ P+++ P Y + +K
Sbjct: 351 VGPAKEEKKEEPKKDDKKKEDDKKDTKKENVIIDPFIFYGAPAYYNHRMK 500
>TC77767 weakly similar to GP|22002137|gb|AAM88621.1 hypothetical protein
{Oryza sativa (japonica cultivar-group)}, partial (36%)
Length = 631
Score = 41.6 bits (96), Expect = 5e-05
Identities = 28/80 (35%), Positives = 46/80 (57%), Gaps = 3/80 (3%)
Frame = +2
Query: 1 MNNQKLRNKAMAIAVGVSGVEGTTIKGDNKDQIEVTGEEIDSVKLASLLRKKFGYADLVS 60
+N K++ KAM G+SGVE +I D KD+ +ID +++ + LR K +A+++S
Sbjct: 95 INEDKIKQKAMKAVSGLSGVESVSI--DMKDKKMTLIGDIDPIRVVAKLR-KLCHAEILS 265
Query: 61 I---EAVGKTEEKKDKEKAE 77
+ + K E KKD +K E
Sbjct: 266 VGPAKEEKKEEPKKDDKKKE 325
>AW126356 similar to GP|22758342|gb| hypothetical protein {Oryza sativa
(japonica cultivar-group)}, partial (17%)
Length = 468
Score = 40.4 bits (93), Expect = 1e-04
Identities = 26/75 (34%), Positives = 49/75 (64%)
Frame = +2
Query: 3 NQKLRNKAMAIAVGVSGVEGTTIKGDNKDQIEVTGEEIDSVKLASLLRKKFGYADLVSIE 62
+ +++ KAM + G+SGVEG ++ +K +I + G ++D V++ S LR K +A+++ I
Sbjct: 68 DDRIKQKAMKMVSGLSGVEGVSMNMKDK-KITLIG-DMDPVRVVSKLR-KLCHAEIIMI- 235
Query: 63 AVGKTEEKKDKEKAE 77
G +E+K++EK E
Sbjct: 236 --GPAKEEKEEEKKE 274
>TC81137 similar to GP|13543783|gb|AAH06040.1 Unknown (protein for MGC:7642)
{Mus musculus}, partial (28%)
Length = 586
Score = 38.9 bits (89), Expect = 3e-04
Identities = 31/104 (29%), Positives = 53/104 (50%), Gaps = 9/104 (8%)
Frame = +3
Query: 1 MNNQKLRNKAMAIAVGVSGVEGTTIKGDNKDQIEVTGEEIDSVKLASLLRKKFGYADLVS 60
++ K++ KAM G+SGVE + D K + + G + D V + + LR K +A+++S
Sbjct: 129 IHEDKIKQKAMKAVSGLSGVESVEVDKDKK--MTLVG-DTDPVLIVAKLR-KLCHAEILS 296
Query: 61 I---EAVGKTEEKKDKEKAE------AIVAWPYVYSSVPHYPVY 95
+ + K E KKD +K E ++ P++Y S P Y
Sbjct: 297 VGPGKEEKKEEPKKDDKKKEDDKKDPPMIINPFMYYSTPTSTYY 428
>TC76522 homologue to SP|O82709|CALX_PEA Calnexin homolog precursor. [Garden
pea] {Pisum sativum}, partial (38%)
Length = 932
Score = 32.0 bits (71), Expect = 0.038
Identities = 19/59 (32%), Positives = 32/59 (54%)
Frame = +3
Query: 17 VSGVEGTTIKGDNKDQIEVTGEEIDSVKLASLLRKKFGYADLVSIEAVGKTEEKKDKEK 75
+S V +K DN+ +I V GEE K+ + + KK + +A G+ E+ K+KE+
Sbjct: 687 LSHVYTAILKPDNELRILVDGEEKKPAKVEAKVEKKTNTERTETSQAGGENEDNKEKEE 863
>TC91295 similar to PIR|T46192|T46192 acetylglutamate kinase-like protein -
Arabidopsis thaliana, partial (47%)
Length = 720
Score = 28.1 bits (61), Expect = 0.55
Identities = 21/48 (43%), Positives = 24/48 (49%), Gaps = 5/48 (10%)
Frame = +1
Query: 6 LRNKAMAIAVGVSGVEG---TTIKGDNKDQIEVTGE--EIDSVKLASL 48
L NKA A AVG+SGV+G T N + GE ID L SL
Sbjct: 538 LINKADATAVGLSGVDGKLLTARPSPNSSNLGFVGEVARIDPAXLRSL 681
>BQ141029 similar to GP|8132441|gb| extensin {Pisum sativum}, partial (54%)
Length = 613
Score = 26.2 bits (56), Expect = 2.1
Identities = 11/19 (57%), Positives = 13/19 (67%)
Frame = +2
Query: 83 PYVYSSVPHYPVYEIKNEP 101
PY YSS P PVY+ K+ P
Sbjct: 182 PYHYSSPPPPPVYKYKSPP 238
>TC77769 similar to PIR|T06397|T06397 isoprenylated protein FP6 - soybean
(fragment), partial (41%)
Length = 1132
Score = 25.8 bits (55), Expect = 2.7
Identities = 11/25 (44%), Positives = 16/25 (64%)
Frame = +2
Query: 1 MNNQKLRNKAMAIAVGVSGVEGTTI 25
+N K++ KAM G+SG+E T I
Sbjct: 227 INEDKIKQKAMKAVSGLSGIELTKI 301
>CA921270 similar to GP|18481964|gb Putative receptor-like protein kinase
{Oryza sativa}, partial (18%)
Length = 842
Score = 25.0 bits (53), Expect = 4.7
Identities = 7/13 (53%), Positives = 10/13 (76%)
Frame = +3
Query: 82 WPYVYSSVPHYPV 94
WPY +S+PH P+
Sbjct: 783 WPYKCNSIPHVPI 821
>TC88328 similar to GP|3287270|emb|CAA70725.1 involved in starch metabalism
{Solanum tuberosum}, partial (29%)
Length = 1719
Score = 25.0 bits (53), Expect = 4.7
Identities = 12/37 (32%), Positives = 21/37 (56%)
Frame = +2
Query: 26 KGDNKDQIEVTGEEIDSVKLASLLRKKFGYADLVSIE 62
+G+N D +E+DS+ SL++K+F +S E
Sbjct: 338 EGENIDDKSTDLKEVDSIPSLSLVKKQFSGRYAISSE 448
>TC91688
Length = 552
Score = 25.0 bits (53), Expect = 4.7
Identities = 18/70 (25%), Positives = 32/70 (45%), Gaps = 4/70 (5%)
Frame = +1
Query: 7 RNKAMAIAVGVSGVEGTTIKGDNKDQIEVTGEEIDSVKLASL----LRKKFGYADLVSIE 62
+N AMAIA +SG + G + + V + ++ L LRKK+GY +
Sbjct: 181 KNLAMAIACSLSGAVLLAV-GAHISYVNVEPQRARTLARDKLVLDTLRKKYGYIPPSEVR 357
Query: 63 AVGKTEEKKD 72
+ + + K+
Sbjct: 358 RMARNDSNKE 387
>TC87077 similar to PIR|A86407|A86407 unknown protein [imported] -
Arabidopsis thaliana, partial (81%)
Length = 1255
Score = 24.6 bits (52), Expect = 6.1
Identities = 11/32 (34%), Positives = 18/32 (55%)
Frame = +3
Query: 17 VSGVEGTTIKGDNKDQIEVTGEEIDSVKLASL 48
+ G+ TT++ K +E GEE D V + +L
Sbjct: 741 IMGLTNTTVEQFCKSAVEPMGEESDHVHITAL 836
>BI311908 similar to GP|10177105|d gene_id:MXC20.14~pir||T13959~similar to
unknown protein {Arabidopsis thaliana}, partial (14%)
Length = 724
Score = 24.6 bits (52), Expect = 6.1
Identities = 12/46 (26%), Positives = 25/46 (54%)
Frame = +2
Query: 15 VGVSGVEGTTIKGDNKDQIEVTGEEIDSVKLASLLRKKFGYADLVS 60
VG+ +E T + N+D ++ TG+ + ++ ++ F + LVS
Sbjct: 32 VGMKPMENTNPEHPNEDMLDDTGDFSEDEQINAVSEVDFNVSTLVS 169
>TC77658 similar to PIR|T46895|T46895 acyl-CoA dehydrogenase (EC 1.3.99.3)
[validated] - Arabidopsis thaliana, partial (94%)
Length = 2886
Score = 24.3 bits (51), Expect = 8.0
Identities = 12/32 (37%), Positives = 18/32 (55%)
Frame = +1
Query: 15 VGVSGVEGTTIKGDNKDQIEVTGEEIDSVKLA 46
+G G+ G TIKG + VTG I + ++A
Sbjct: 412 LGELGIAGGTIKGYGCPGLSVTGSAIATAEVA 507
>TC90272 similar to PIR|T45686|T45686 receptor-like protein kinase homolog -
Arabidopsis thaliana, partial (58%)
Length = 941
Score = 24.3 bits (51), Expect = 8.0
Identities = 9/22 (40%), Positives = 14/22 (62%)
Frame = +3
Query: 36 TGEEIDSVKLASLLRKKFGYAD 57
TG EID +++ ++ FGY D
Sbjct: 375 TGPEIDQTHVSTAVKGSFGYLD 440
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.310 0.129 0.353
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 2,577,047
Number of Sequences: 36976
Number of extensions: 24547
Number of successful extensions: 118
Number of sequences better than 10.0: 34
Number of HSP's better than 10.0 without gapping: 115
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 116
length of query: 106
length of database: 9,014,727
effective HSP length: 82
effective length of query: 24
effective length of database: 5,982,695
effective search space: 143584680
effective search space used: 143584680
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.7 bits)
S2: 50 (23.9 bits)
Medicago: description of AC144618.2