BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC144564.12 + phase: 0
(357 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC85719 similar to PIR|T49940|T49940 hypothetical protein F17I14... 642 0.0
TC85717 similar to SP|Q01899|HS7M_PHAVU Heat shock 70 kDa protei... 87 1e-17
BQ141697 similar to GP|18159237|gb| conserved within P. aerophil... 32 0.38
BQ152864 31 0.64
TC85228 weakly similar to GP|19911209|dbj|BAB86931. glucosyltran... 30 1.9
TC85231 similar to GP|13508844|emb|CAC35167. arbutin synthase {R... 30 1.9
AW288078 30 1.9
TC87817 homologue to GP|20161224|dbj|BAB90151. katanin p60 subun... 30 1.9
TC77915 similar to PIR|D96815|D96815 probable gamma-glutamyl hyd... 29 2.4
CA920824 29 3.2
TC80732 similar to GP|21553690|gb|AAM62783.1 unknown {Arabidopsi... 28 4.2
TC86826 similar to PIR|F96568|F96568 probable lipase 20450-2164... 28 5.4
TC89942 similar to GP|20259233|gb|AAM14332.1 putative ankyrin pr... 28 7.1
TC91597 similar to GP|21323350|dbj|BAB97978. Hypothetical protei... 28 7.1
TC77916 similar to GP|17979073|gb|AAL49804.1 putative gamma glut... 28 7.1
TC80340 similar to PIR|T47946|T47946 prolyl aminopeptidase-like ... 28 7.1
AJ388777 homologue to SP|P27993|LGB2 Leghemoglobin 2. [Barrel me... 28 7.1
TC76877 leghemoglobin 28 7.1
BQ151241 GP|19917424|gb conserved hypothetical protein {Methanos... 27 9.3
>TC85719 similar to PIR|T49940|T49940 hypothetical protein F17I14.230 -
Arabidopsis thaliana, partial (62%)
Length = 1970
Score = 642 bits (1657), Expect = 0.0
Identities = 333/362 (91%), Positives = 339/362 (92%), Gaps = 5/362 (1%)
Frame = +2
Query: 1 MKKNIQFSFPPCVEIGYRSLRSDAALEAIAKASEDKVPNIVLYNYPSFSGAFSSLFAHLF 60
MKKNIQFSFPPCVEIGYRSLRSDAALEAIAKASEDKVPNIVLYNYPSFSGAFSSLFAHLF
Sbjct: 347 MKKNIQFSFPPCVEIGYRSLRSDAALEAIAKASEDKVPNIVLYNYPSFSGAFSSLFAHLF 526
Query: 61 HTRHNLPSLSLPFSSVPSLAFSCYNI---GLKICALKAFRHAICLTFFL--QTNSLIIGF 115
HTRHNLPSLSLPFSSVPSLAF ++ L+ C L F F L Q+N IIGF
Sbjct: 527 HTRHNLPSLSLPFSSVPSLAFRVEDLCIESLQTCYLLDFLPPNEFIFKLSHQSNCKIIGF 706
Query: 116 DHRKSVLSQIPSTNECPENIMINLNHEKSSSRAVYEYFTDKHEDIKTSNGVVPSLVDSKD 175
DHRKSVLSQIPSTNECPENIMINLNHEKSSSRAVYEYFTDKHEDIKTSNGVVPSLVDSKD
Sbjct: 707 DHRKSVLSQIPSTNECPENIMINLNHEKSSSRAVYEYFTDKHEDIKTSNGVVPSLVDSKD 886
Query: 176 KGRVELILKYIEDADLRHWSLPDIKPFNIGLSEWRSRFSCISNPYMFKQLLELSVEELIA 235
KGRVELILKYIEDADLRHWSLPDIKPFNIGLSEWRSRFSCISNPYMFKQLLELSVEELIA
Sbjct: 887 KGRVELILKYIEDADLRHWSLPDIKPFNIGLSEWRSRFSCISNPYMFKQLLELSVEELIA 1066
Query: 236 KGNSSLLARRNAASKLLDKVFRVRLGRGFYGECLGVRADGNSNLSDEIGMLLSVKSASIG 295
KGNSSLLAR+NAASKLLDKVFRVRLGRGFYGECLGVRADGNSNLSDEIGMLLSVKSA+IG
Sbjct: 1067KGNSSLLARQNAASKLLDKVFRVRLGRGFYGECLGVRADGNSNLSDEIGMLLSVKSAAIG 1246
Query: 296 LRPIGAVIFMQRNNLKMCLRSSDNATDTSEVAKAYGGGGSASSSSFIIRMDEYNQWLSAN 355
LRPIGAVIFMQRNNLKMCLRSSDNATDTSEVAKAYGGGGSASSSSFIIRMDEYNQWLSAN
Sbjct: 1247LRPIGAVIFMQRNNLKMCLRSSDNATDTSEVAKAYGGGGSASSSSFIIRMDEYNQWLSAN 1426
Query: 356 SL 357
SL
Sbjct: 1427SL 1432
>TC85717 similar to SP|Q01899|HS7M_PHAVU Heat shock 70 kDa protein
mitochondrial precursor. [Kidney bean French bean]
{Phaseolus vulgaris}, partial (5%)
Length = 677
Score = 87.0 bits (214), Expect = 1e-17
Identities = 42/44 (95%), Positives = 43/44 (97%)
Frame = +1
Query: 1 MKKNIQFSFPPCVEIGYRSLRSDAALEAIAKASEDKVPNIVLYN 44
MKKNIQFSFPPCVEIGYRSLRSDAALEAIAKASEDKV N+VLYN
Sbjct: 544 MKKNIQFSFPPCVEIGYRSLRSDAALEAIAKASEDKVSNVVLYN 675
>BQ141697 similar to GP|18159237|gb| conserved within P. aerophilum
{Pyrobaculum aerophilum}, partial (4%)
Length = 1368
Score = 32.0 bits (71), Expect = 0.38
Identities = 14/35 (40%), Positives = 20/35 (57%)
Frame = +2
Query: 34 EDKVPNIVLYNYPSFSGAFSSLFAHLFHTRHNLPS 68
E + PN +LY S + SSLF H + T + +PS
Sbjct: 173 EQETPNPILYRITSLISSLSSLFIHPYRTPYFIPS 277
>BQ152864
Length = 533
Score = 31.2 bits (69), Expect = 0.64
Identities = 19/71 (26%), Positives = 30/71 (41%)
Frame = +2
Query: 45 YPSFSGAFSSLFAHLFHTRHNLPSLSLPFSSVPSLAFSCYNIGLKICALKAFRHAICLTF 104
Y S G F+++ LFH H F SVP L + + + L +C + + +
Sbjct: 311 YLSTQGRFNNIIMVLFHLNHVKS*FKCFFLSVPLLFYIVFTLNLILCTFRQYLMLLWNHV 490
Query: 105 FLQTNSLIIGF 115
L N L + F
Sbjct: 491 ELNRNFLGVAF 523
>TC85228 weakly similar to GP|19911209|dbj|BAB86931. glucosyltransferase-13
{Vigna angularis}, partial (31%)
Length = 3139
Score = 29.6 bits (65), Expect = 1.9
Identities = 14/43 (32%), Positives = 25/43 (57%), Gaps = 3/43 (6%)
Frame = -1
Query: 298 PIGAVIFMQRNN---LKMCLRSSDNATDTSEVAKAYGGGGSAS 337
P+G +I + NN + +CLR +N +S + ++G GG+ S
Sbjct: 2080 PVGPIIRNESNNEANMSVCLRWLENQPPSSVIFVSFGSGGTLS 1952
>TC85231 similar to GP|13508844|emb|CAC35167. arbutin synthase {Rauvolfia
serpentina}, partial (34%)
Length = 1602
Score = 29.6 bits (65), Expect = 1.9
Identities = 15/43 (34%), Positives = 25/43 (57%), Gaps = 3/43 (6%)
Frame = +3
Query: 298 PIGAVIFMQRNN---LKMCLRSSDNATDTSEVAKAYGGGGSAS 337
P+G +I + NN + MCLR +N +S + ++G GG+ S
Sbjct: 756 PVGPMIRNESNNEANMSMCLRWLENQQPSSVLFVSFGSGGTLS 884
>AW288078
Length = 494
Score = 29.6 bits (65), Expect = 1.9
Identities = 27/108 (25%), Positives = 44/108 (40%)
Frame = +2
Query: 97 RHAICLTFFLQTNSLIIGFDHRKSVLSQIPSTNECPENIMINLNHEKSSSRAVYEYFTDK 156
RH + + N ++ +H L +IP E P I K + YFTD
Sbjct: 14 RHLNRINSIIIENGTLLSLEHL--TLEKIPQLKEVPIGI-------KHMHKLKDIYFTDM 166
Query: 157 HEDIKTSNGVVPSLVDSKDKGRVELILKYIEDADLRHWSLPDIKPFNI 204
S +D DKG+ I+K++ +RHW P++ ++I
Sbjct: 167 PAQFVES-------IDP-DKGQNYSIIKHVPLVFIRHWYGPNLYDYDI 286
>TC87817 homologue to GP|20161224|dbj|BAB90151. katanin p60 subunit A 1-like
{Oryza sativa (japonica cultivar-group)}, partial (25%)
Length = 768
Score = 29.6 bits (65), Expect = 1.9
Identities = 17/55 (30%), Positives = 31/55 (55%)
Frame = +2
Query: 138 NLNHEKSSSRAVYEYFTDKHEDIKTSNGVVPSLVDSKDKGRVELILKYIEDADLR 192
N + + +S RA+Y+ F + ++ +NG P+ VD K K + +L E A++R
Sbjct: 284 NSHGKVTSDRAIYDQFQSQGQNPTHTNGFGPNGVDEKPK---KSLLPPFESAEMR 439
>TC77915 similar to PIR|D96815|D96815 probable gamma-glutamyl hydrolase
[imported] - Arabidopsis thaliana, partial (84%)
Length = 1361
Score = 29.3 bits (64), Expect = 2.4
Identities = 16/49 (32%), Positives = 25/49 (50%), Gaps = 5/49 (10%)
Frame = -2
Query: 3 KNIQFS-----FPPCVEIGYRSLRSDAALEAIAKASEDKVPNIVLYNYP 46
KN+Q++ PPCVE +R +L+AI S D N++ + P
Sbjct: 892 KNLQYTESFEWHPPCVEYFLVPIRRHFSLDAIGSQSRDSYVNVLWKHKP 746
>CA920824
Length = 744
Score = 28.9 bits (63), Expect = 3.2
Identities = 15/37 (40%), Positives = 21/37 (56%)
Frame = +2
Query: 53 SSLFAHLFHTRHNLPSLSLPFSSVPSLAFSCYNIGLK 89
SS++ LFH L SL +PFS+ +F C G+K
Sbjct: 362 SSIYPVLFHVLILLDSLPVPFSTSNGTSFLCTMKGMK 472
>TC80732 similar to GP|21553690|gb|AAM62783.1 unknown {Arabidopsis
thaliana}, partial (16%)
Length = 760
Score = 28.5 bits (62), Expect = 4.2
Identities = 16/50 (32%), Positives = 25/50 (50%), Gaps = 1/50 (2%)
Frame = +3
Query: 93 LKAFRHAICLTFFLQTNSLIIGFDHRKSVLSQIPSTNECP-ENIMINLNH 141
L R A+ L T L + ++ Q P+T++CP +N+ NLNH
Sbjct: 9 LLLLRSALALPHISSTRLLCLRV*SDRNFFLQNPNTSKCPNQNLKTNLNH 158
>TC86826 similar to PIR|F96568|F96568 probable lipase 20450-21648
[imported] - Arabidopsis thaliana, partial (93%)
Length = 1214
Score = 28.1 bits (61), Expect = 5.4
Identities = 15/51 (29%), Positives = 25/51 (48%)
Frame = +2
Query: 144 SSSRAVYEYFTDKHEDIKTSNGVVPSLVDSKDKGRVELILKYIEDADLRHW 194
SSS+ +YE K + +K G+ SL+ + L+L+ D+R W
Sbjct: 827 SSSKLLYEKAESKDKTLKLYEGMYHSLIQGEPDESANLVLR-----DMREW 964
>TC89942 similar to GP|20259233|gb|AAM14332.1 putative ankyrin protein
{Arabidopsis thaliana}, partial (24%)
Length = 825
Score = 27.7 bits (60), Expect = 7.1
Identities = 17/61 (27%), Positives = 27/61 (43%)
Frame = +1
Query: 296 LRPIGAVIFMQRNNLKMCLRSSDNATDTSEVAKAYGGGGSASSSSFIIRMDEYNQWLSAN 355
LRP G VIF + + ++ A VA A G S S ++ + + WL++
Sbjct: 268 LRPTGFVIFRDKQPMIDFVKKYLTALHWEAVATADSGSDSVQDSDEVVFIIQKKLWLTSG 447
Query: 356 S 356
S
Sbjct: 448 S 450
>TC91597 similar to GP|21323350|dbj|BAB97978. Hypothetical protein
{Corynebacterium glutamicum ATCC 13032}, partial (5%)
Length = 718
Score = 27.7 bits (60), Expect = 7.1
Identities = 20/55 (36%), Positives = 26/55 (46%)
Frame = -2
Query: 42 LYNYPSFSGAFSSLFAHLFHTRHNLPSLSLPFSSVPSLAFSCYNIGLKICALKAF 96
L + SFS F S FA + S + FS + FSC++IGL C L F
Sbjct: 549 LMGFSSFSADFFSDFA--------IGSSTGFFSCFSTGFFSCFSIGLSSCFLAGF 409
>TC77916 similar to GP|17979073|gb|AAL49804.1 putative gamma glutamyl
hydrolase {Arabidopsis thaliana}, partial (82%)
Length = 1695
Score = 27.7 bits (60), Expect = 7.1
Identities = 13/39 (33%), Positives = 20/39 (50%)
Frame = -2
Query: 10 PPCVEIGYRSLRSDAALEAIAKASEDKVPNIVLYNYPSF 48
PPCVE +R +L+AI S D N++ + P +
Sbjct: 1106 PPCVEYLVCPIRKHFSLDAIGSQSPDSYVNVLWKHKPCY 990
>TC80340 similar to PIR|T47946|T47946 prolyl aminopeptidase-like protein -
Arabidopsis thaliana, partial (41%)
Length = 907
Score = 27.7 bits (60), Expect = 7.1
Identities = 20/52 (38%), Positives = 24/52 (45%), Gaps = 3/52 (5%)
Frame = -2
Query: 117 HRKSVLSQIPSTNECPENIMINLNHEKSSSRAVYEYF---TDKHEDIKTSNG 165
H K L Q+P PE +I N SSR VYE +HE +TS G
Sbjct: 489 HNKRRLQQVPCCCSKPECHIIGAN---PSSRTVYELHL*TVSRHESHQTSMG 343
>AJ388777 homologue to SP|P27993|LGB2 Leghemoglobin 2. [Barrel medic]
{Medicago truncatula}, complete
Length = 549
Score = 27.7 bits (60), Expect = 7.1
Identities = 19/65 (29%), Positives = 30/65 (45%)
Frame = -2
Query: 47 SFSGAFSSLFAHLFHTRHNLPSLSLPFSSVPSLAFSCYNIGLKICALKAFRHAICLTFFL 106
++S S L + + H N S++L F ++ + C LK HA+C FL
Sbjct: 260 NYSPCCSELNS*IAHHSKNFFSMTLEFRTILYSS----------CVLKKREHALCCRGFL 111
Query: 107 QTNSL 111
Q NS+
Sbjct: 110 QYNSV 96
>TC76877 leghemoglobin
Length = 637
Score = 27.7 bits (60), Expect = 7.1
Identities = 19/65 (29%), Positives = 30/65 (45%)
Frame = -1
Query: 47 SFSGAFSSLFAHLFHTRHNLPSLSLPFSSVPSLAFSCYNIGLKICALKAFRHAICLTFFL 106
++S S L + + H N S++L F ++ + C LK HA+C FL
Sbjct: 286 NYSPCCSELNS*IAHHSKNFFSMTLEFRTILYSS----------CVLKKREHALCCRGFL 137
Query: 107 QTNSL 111
Q NS+
Sbjct: 136 QYNSV 122
>BQ151241 GP|19917424|gb conserved hypothetical protein {Methanosarcina
acetivorans str. C2A}, partial (3%)
Length = 560
Score = 27.3 bits (59), Expect = 9.3
Identities = 13/36 (36%), Positives = 20/36 (55%)
Frame = +1
Query: 45 YPSFSGAFSSLFAHLFHTRHNLPSLSLPFSSVPSLA 80
+PS+S +S F HL + + ++LP S P LA
Sbjct: 1 FPSYSPCITSPFLHLAYYLYRPAPIALPHSCSPCLA 108
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.320 0.135 0.393
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 11,743,986
Number of Sequences: 36976
Number of extensions: 175852
Number of successful extensions: 979
Number of sequences better than 10.0: 38
Number of HSP's better than 10.0 without gapping: 969
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 976
length of query: 357
length of database: 9,014,727
effective HSP length: 97
effective length of query: 260
effective length of database: 5,428,055
effective search space: 1411294300
effective search space used: 1411294300
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 59 (27.3 bits)
Medicago: description of AC144564.12